Publications by authors named "Zheng Xiang"

405 Publications

Characteristics of size-segregated aerosols emitted from an aerobic moving bed biofilm reactor at a full-scale wastewater treatment plant.

J Hazard Mater 2021 08 20;416:125833. Epub 2021 Apr 20.

School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266033, PR China. Electronic address:

Aerosol emissions from wastewater treatment plants (WWTPs) have been associated with health reverberation but studies about characteristics of size-segregated aerosol particulate matter (PM) are scarce. In this study, the measurement of particulate number size distribution in the range of < 10 µm, and the collection of PM, PM and PM, were conducted from an aerobic moving bed biofilm reactor (MBBR) at a full-scale WWTP. MBBR aerosols showed a unimodal number size distribution, with the majority of particles (>94%) in the ultrafine size range (<100 nm). For toxic metal(loid)s or potential pathogens, significant differences were found within MBBR aerosols (PM, PM, and PM), and also between MBBR aerosols and wastewater. Both wastewater and ambient air had important source contributions for MBBR aerosols. The compositions of toxic metal(loid)s in PM, and the populations of potential bacterial or fungal pathogens in PM and PM, were dominated by that from wastewater. Compared to PM and PM, PM had the highest aerosolization potential for the toxic metal(loid)s of As, Cd, Co, Cr, Li, Mn, Ni, U, and Zn, and the genera of Acinetobacter, Pseudomonas and Fusarium. Due to the size-segregated specialty, targeted measures should be employed to reduce the health risks. CAPSULE: The compositions of toxic metal(loid)s in PM, and the populations of potential pathogens in PM and PM, were dominated by that from wastewater.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125833DOI Listing
August 2021

Epstein-Barr virus EBNA2 phase separation regulates cancer-associated alternative RNA splicing patterns.

Clin Transl Med 2021 Aug;11(8):e504

Hunan Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, China.

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http://dx.doi.org/10.1002/ctm2.504DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351520PMC
August 2021

3D bioprinting of conductive hydrogel for enhanced myogenic differentiation.

Regen Biomater 2021 Oct 14;8(5):rbab035. Epub 2021 Aug 14.

Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen 361021, P. R. China.

Recently, hydrogels have gained enormous interest in three-dimensional (3D) bioprinting toward developing functional substitutes for tissue remolding. However, it is highly challenging to transmit electrical signals to cells due to the limited electrical conductivity of the bioprinted hydrogels. Herein, we demonstrate the 3D bioprinting-assisted fabrication of a conductive hydrogel scaffold based on poly-3,4-ethylene dioxythiophene (PEDOT) nanoparticles (NPs) deposited in gelatin methacryloyl (GelMA) for enhanced myogenic differentiation of mouse myoblasts (C2C12 cells). Initially, PEDOT NPs are dispersed in the hydrogel uniformly to enhance the conductive property of the hydrogel scaffold. Notably, the incorporated PEDOT NPs showed minimal influence on the printing ability of GelMA. Then, C2C12 cells are successfully encapsulated within GelMA/PEDOT conductive hydrogels using 3D extrusion bioprinting. Furthermore, the proliferation, migration and differentiation efficacies of C2C12 cells in the highly conductive GelMA/PEDOT composite scaffolds are demonstrated using various investigations of live/dead staining, F-actin staining, desmin and myogenin immunofluorescence staining. Finally, the effects of electrical signals on the stimulation of the scaffolds are investigated toward the myogenic differentiation of C2C12 cells and the formation of myotubes . Collectively, our findings demonstrate that the fabrication of the conductive hydrogels provides a feasible approach for the encapsulation of cells and the regeneration of the muscle tissue.
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http://dx.doi.org/10.1093/rb/rbab035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363764PMC
October 2021

Engineered T Cell Therapy for Gynecologic Malignancies: Challenges and Opportunities.

Front Immunol 2021 27;12:725330. Epub 2021 Jul 27.

Department of Immunology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.

Gynecologic malignancies, mainly including ovarian cancer, cervical cancer and endometrial cancer, are leading causes of death among women worldwide with high incidence and mortality rate. Recently, adoptive T cell therapy (ACT) using engineered T cells redirected by genes which encode for tumor-specific T cell receptors (TCRs) or chimeric antigen receptors (CARs) has demonstrated a delightful potency in B cell lymphoma treatment. Researches impelling ACT to be applied in treating solid tumors like gynecologic tumors are ongoing. This review summarizes the preclinical research and clinical application of engineered T cells therapy for gynecologic cancer in order to arouse new thoughts for remedies of this disease.
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http://dx.doi.org/10.3389/fimmu.2021.725330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353443PMC
July 2021

Ubiquitin-Like Protein UBD Promotes Cell Proliferation in Colorectal Cancer by Facilitating p53 Degradation.

Front Oncol 2021 14;11:691347. Epub 2021 Jul 14.

Department of Gastrointestinal Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Purpose: Ubiquitin D (UBD) is a member of the ubiquitin-like modifier (UBL) family and is highly expressed in a variety of cancers including colorectal cancer (CRC). However, the mechanisms of its regulatory roles in CRC are largely elusive. In this study, we revealed the effect of UBD on the proliferation of CRC.

Methods: The expression of UBD in clinical tissue samples of CRC and seven CRC cell lines was detected using qRT-PCR, immunohistochemistry (IHC) and Western blotting. CCK-8, colony formation, EdU and flow cytometry assays were used to detect the functional changes of CRC cells transfected with UBD stable expression plasmids . A xenograft model was constructed to assess the effect of UBD on the growth of CRC cells . The connection between UBD and p53 was analyzed using Western blotting, immunoprecipitation, proteasome inhibition assay and Cycloheximide (CHX) chase assay.

Results: UBD was overexpressed in CRC tumor tissues compared with nontumor tissues, and its overexpression was positively associated with the tumor size and TNM stage of CRC patients. Functionally, UBD significantly accelerated CRC cell viability and proliferation and promoted tumorigenesis . Mechanistically, UBD interacted with p53 in CRC cells, downregulated the expression of p53 by regulating its degradation, shortened the p53 half-life, thereby further affecting the decrease in p21 and the increase in Cyclin D1, Cyclin E, CDK2, CDK4 and CDK6. Moreover, experiments showed that UBD-induced tumor growth in nude mice was dependent on a decrease in p53.

Conclusions: Our study proved that UBD mediates the degradation of p53, thereby facilitating the growth of CRC cells and ultimately promoting the progression of CRC. Therefore, UBD may be a potential therapeutic target and a promising prognostic biomarker for CRC.
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http://dx.doi.org/10.3389/fonc.2021.691347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327751PMC
July 2021

A Sanger sequencing protocol for SARS-CoV-2 S-gene.

Influenza Other Respir Viruses 2021 Aug 3. Epub 2021 Aug 3.

Worldwide Influenza Centre (WIC: a WHO Collaborating Centre for Reference and Research on Influenza), The Francis Crick Institute, London, UK.

We describe a Sanger sequencing protocol for SARS-CoV-2 S-gene the Spike (S)-glycoprotein product of which, composed of receptor-binding (S1) and membrane fusion (S2) segments, is the target of vaccines used to combat COVID-19. The protocol can be used in laboratories with basic Sanger sequencing capabilities and allows rapid "at source" screening for SARS-CoV-2 variants, notably those of concern. The protocol has been applied for surveillance, with clinical specimens collected in either nucleic acid preservation lysis-mix or virus transport medium, and research involving cultured viruses, and can yield data of public health importance in a timely manner.
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http://dx.doi.org/10.1111/irv.12892DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8447197PMC
August 2021

Effects of acute hypoxia and reoxygenation on oxygen sensors, respiratory metabolism, oxidative stress, and apoptosis in hybrid yellow catfish "Huangyou-1".

Fish Physiol Biochem 2021 Jul 27. Epub 2021 Jul 27.

College of Marine Science and Engineering, Jiangsu Province Engineering Research Center for Aquatic Animals Breeding and Green Efficient Aquacultural Technology, Nanjing Normal University, Nanjing, 210023, Jiangsu, China.

The regulation mechanism of the hybrid yellow catfish "Huangyou-1" was assessed under conditions of hypoxia and reoxygenation by examination of oxygen sensors and by monitoring respiratory metabolism, oxidative stress, and apoptosis. The expressions of genes related to oxygen sensors (HIF-1α, HIF-2α, VHL, HIF-1β, PHD2, and FIH-1) were upregulated in the brain and liver during hypoxia, and recovered compared with control upon reoxygenation. The expressions of genes related to glycolysis (HK1, PGK1, PGAM2, PFK, and LDH) were increased during hypoxia and then recovered compared with control upon reoxygenation. The mRNA levels of CS did not change during hypoxia in the brain and liver, but increased during reoxygenation. The mRNA levels of SDH decreased significantly only in the liver during hypoxia, but later increased compared with control upon reoxygenation in both tissues. Under hypoxic conditions, the expressions of genes related to oxidative stress (SOD1, SOD2, GSH-Px, and CAT) and the activity of antioxidant enzymes (SOD, CAT, and GSH-Px) and MDA were upregulated compared with control. The expressions of genes related to apoptosis (Apaf-1, Bax, Caspase 3, Caspase 9, and p53) were higher than those in control during hypoxic exposure, while the expressions of Bcl-2 and Cyt C were decreased. The findings of the transcriptional analyses will provide insights into the molecular mechanisms of hybrid yellow catfish "Huangyou-1" under conditions of hypoxia and reoxygenation. Overall, these findings showed that oxygen sensors of "Huangyou-1" are potentially useful biomarkers of environmental hypoxic exposure. Together with genes related to respiratory metabolism, oxidative stress and apoptosis occupy a quite high position in enhancing hypoxia tolerance. Our findings provided new insights into the molecular regulatory mechanism of hypoxia in "Huangyou-1."
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http://dx.doi.org/10.1007/s10695-021-00989-8DOI Listing
July 2021

Expression of ACKR4 demarcates the "peri-marginal sinus," a specialized vascular compartment of the splenic red pulp.

Cell Rep 2021 Jul;36(2):109346

Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany; Cluster of Excellence RESIST (EXC 2155), Hannover Medical School, 30625 Hannover, Germany. Electronic address:

The spleen comprises defined microanatomical compartments that uniquely contribute to its diverse host defense functions. Here, we identify a vascular compartment within the red pulp of the spleen delineated by expression of the atypical chemokine receptor 4 (ACKR4) in endothelial cells. ACKR4-positive vessels form a three-dimensional sinusoidal network that connects via shunts to the marginal sinus and tightly surrounds the outer perimeter of the marginal zone. Endothelial cells lining this vascular compartment express ACKR4 as part of a distinct gene expression profile. We show that T cells enter the spleen largely through this peri-marginal sinus and initially localize extravascularly around these vessels. In the absence of ACKR4, homing of T cells into the spleen and subsequent migration into T cell areas is impaired, and organization of the marginal zone is severely affected. Our data delineate the splenic peri-marginal sinus as a compartment that supports spleen homing of T cells.
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http://dx.doi.org/10.1016/j.celrep.2021.109346DOI Listing
July 2021

Polymorphism of Antifolate Drug Resistance in From Local Residents and Migrant Workers Returned From the China-Myanmar Border.

Front Cell Infect Microbiol 2021 24;11:683423. Epub 2021 Jun 24.

Department of Pathogen Biology and Immunology, Kunming Medical University, Kunming, China.

Drug-resistant  malaria impedes efforts to control, eliminate, and ultimately eradicate malaria in Southeast Asia. resistance to antifolate drugs derives from point mutations in specific parasite genes, including the dihydropteroate synthase (), dihydrofolate reductase (), and GTP cyclohydrolase I () genes. This study aims to investigate the prevalence and spread of drug resistance markers in populating the China-Myanmar border. Blood samples were collected from symptomatic patients with acute infection. Samples with single-clone infections were sequenced for and genesand genotyped for 6 flanking microsatellite markers. Copy number variation in the gene was also examined. Polymorphisms were observed in six different codons of the  gene (382, 383, 512, 549, 553, and 571) and six different codons of the  gene (13, 57, 58, 61, 99, 117) in two study sites. The quadruple mutant haplotypes 57I/L/58R/61M/117T of gene were the most common (comprising 76% of cases in Myitsone and 43.7% of case in Laiza). The double mutant haplotype 383G/553G of  gene was also prevalent at each site (40.8% and 31%). Microsatellites flanking the gene differentiated clinical samples from wild type and quadruple mutant genotypes ( = 0.259-0.3036), as would be expected for a locus undergoing positive selection. The lack of copy number variation of suggests that SP-resistant may harbor alternative mechanisms to secure sufficient folate.
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http://dx.doi.org/10.3389/fcimb.2021.683423DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8265503PMC
July 2021

Clinical value of INSL3 in the diagnosis and development of diabetic nephropathy.

J Clin Lab Anal 2021 Sep 7;35(9):e23898. Epub 2021 Jul 7.

Department of Health Management Centre, The First Affiliated Hospital of Soochow University, Suzhou, China.

Background: Insulin-like factor 3 (INSL3) was stated to be an essential regulator in many diseases. This present study aimed to explore the underlying mechanisms of INSL3 in diabetic nephropathy (DN).

Methods: The serum samples were obtained from 121 DN patients, 67 T2DM patients, and 44 healthy controls. Twenty SD rats were used to establish the DN model in vivo. Quantitative PCR (qPCR) and Western blot were completed to analyze the INSL3 expression in cells, serum samples, and kidney of the rats. The structure of kidney was analyzed by HE staining. The diagnostic values of INSL3 in DN were determined by receiver operating characteristic (ROC) assay. Then, Spearman's correlation analysis was executed to verify the association between INSL3 and glomerular filtration rate (eGFR). Finally, the proliferation and apoptosis status of transfected cells were analyzed by MTT, flow cytometry, and Hoechst33258 staining assay.

Results: We found that INSL3 expression was up-regulated in DN patients and SV40-MES-13 cells. Furthermore, the correlation analysis elucidated that INSL3 expression was negatively correlated with DN diagnosis golden criterion eGFR. INSL3 knockdown promoted the proliferation rate and inhibited the apoptosis rate of SV40-MES-13 cells after high-glucose treatment. Finally, the INSL3 expression and fast blood glucose were up-regulated in DN rats.

Conclusions: Collectively, this study demonstrated the clinical significance of INSL3 in diagnosing and developing DN.
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http://dx.doi.org/10.1002/jcla.23898DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418484PMC
September 2021

Microscopic transduodenal excision of an ampullary adenoma: A case report and review of the literature.

World J Clin Cases 2021 Jun;9(18):4844-4851

Department of General Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, Zhejiang Province, China.

Background: Transduodenal local excision is an alternative treatment approach for benign ampullary tumors. However, this procedure has technical difficulties, especially during reconstruction of the pancreaticobiliary ducts. An operating microscope has been widely used by surgeons for delicate surgery due to its major advantages of magnification, illumination, and stereoscopic view. The application of an operating microscope in transduodenal excision of ampullary tumors has not been reported.

Case Summary: A 55-year-old woman was admitted for investigation of recurrent upper abdominal pain. Physical examination and laboratory tests found no abnormalities. Imaging identified a large mass in the descending part of the duodenum. Esophagogastroduodenoscopy revealed a 3.5-cm-sized villous growth over the major duodenal papilla. Pathology of the endoscopic biopsy indicated a villous adenoma with low-grade dysplasia. Microscopic transduodenal excision of the ampullary tumor was performed. The final pathological diagnosis was villous-tubular adenoma with low-grade dysplasia. The patient was discharged on postoperative day 12 after an uneventful recovery. Endoscopic retrograde cholangiopancreatography was performed 3 mo postoperatively and showed no bile duct or pancreatic duct strictures and no tumor recurrence. The patient is continuing follow-up at our clinic and remains well.

Conclusion: Operating microscope-assisted transduodenal local excision is a feasible and effective option for benign ampullary tumors.
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http://dx.doi.org/10.12998/wjcc.v9.i18.4844DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8223850PMC
June 2021

Laparoscopic spleen-preserving distal pancreatectomy for epidermoid cyst in an intrapancreatic accessory spleen: A case report.

Medicine (Baltimore) 2021 Jul;100(26):e26379

Department of General Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009.

Rationale: Pancreatic tail cystic lesions are increasingly encountered in clinical practice, however, it is difficult to make a correct diagnosis preoperatively because there are many types of pancreatic neoplastic and non-neoplastic cysts. Epidermoid cyst in an intrapancreatic accessory spleen (ECIPAS) is a rare non-neoplastic cyst locating in the pancreatic tail, and it is commonly misdiagnosed as another cystic neoplasm.

Patient Concerns: A 51-year-old man was admitted for investigation of abdominal pain. The physical examination and laboratory tests found no abnormalities, except for an elevation of carbohydrate antigen (CA)19-9. Imaging revealed a cystic lesion within the pancreatic tail, and the solid component surrounding the cyst was enhanced similarly to those of the splenic tissue.

Diagnosis: ECIPAS was diagnosed based on the pathology after surgery. The mass was composed of a cyst and brown solid spleen-like tissue. The microscopic analysis demonstrated that the solid component was accessory splenic tissue, and the cyst wall was lined with a thin stratified squamous epithelium.

Interventions: Laparoscopic spleen-preserving distal pancreatectomy was performed.

Outcomes: The patient was discharged on day 5 postoperatively after an uneventful recovery. CA19-9 returned to normal after surgery. During a 2-years follow-up, there was no evidence of tumor recurrence.

Lessons: Although rare ECIPAS should be considered in the differential diagnosis of pancreatic tail cystic lesions, and the typical imaging features might facilitate the preoperative diagnosis. Laparoscopic distal pancreatectomy is a safe and effective approach for treating ECIPAS.
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http://dx.doi.org/10.1097/MD.0000000000026379DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257839PMC
July 2021

Concurrent Modified Constant Modulus Algorithm and Decision Directed Scheme With Barzilai-Borwein Method.

Front Neurorobot 2021 10;15:699221. Epub 2021 Jun 10.

Guangdong Shenglu Telecommunication Tech. Co., Ltd, Foshan, China.

At present, in robot technology, remote control of robot is realized by wireless communication technology, and data anti-interference in wireless channel becomes a very important part. Any wireless communication system has an inherent multi-path propagation problem, which leads to the expansion of generated symbols on a time scale, resulting in symbol overlap and Inter-symbol Interference (ISI). ISI in the signal must be removed and the signal restores to its original state at the time of transmission or becomes as close to it as possible. Blind equalization is a popular equalization method for recovering transmitted symbols of superimposed noise without any pilot signal. In this work, we propose a concurrent modified constant modulus algorithm (MCMA) and the decision-directed scheme (DDS) with the Barzilai-Borwein (BB) method for the purpose of blind equalization of wireless communications systems (WCS). The BB method, which is two-step gradient method, has been widely employed to solve multidimensional unconstrained optimization problems. Considering the similarity of equalization process and optimization process, the proposed algorithm combines existing blind equalization algorithm and Barzilai-Borwein method, and concurrently operates a MCMA equalizer and a DD equalizer. After that, it modifies the DD equalizer's step size (SS) by the BB method. Theoretical investigation was involved and it demonstrated rapid convergence and improved equalization performance of the proposed algorithm compared with the original one. Additionally, the simulation results were consistent with the proposed technique.
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http://dx.doi.org/10.3389/fnbot.2021.699221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222806PMC
June 2021

The Protective Role of the Long Pentraxin PTX3 in Spontaneously Hypertensive Rats with Heart Failure.

Cardiovasc Toxicol 2021 Oct 25;21(10):808-819. Epub 2021 Jun 25.

Department of Gastroenterology, Renmin Hospital, Hubei University of Medicine, Shiyan, 442000, Hubei, China.

Pentraxin 3 (PTX3) is synthesized locally and released into the circulation, reflecting local inflammation in the cardiovascular system. Therefore, we conducted a study to explore the effect of PTX3 in spontaneously hypertensive heart failure (SHHF) rats. Sprague Dawley (SD) and SHHF rats were treated with recombinant PTX3 protein, and the blood pressure (BP) and echocardiographic parameters were collected. Radioimmunoassay, enzyme immunoassay and enzyme-linked immunosorbent assay (ELISA) were applied to detect plasma levels of atrial/B-type natriuretic peptide (ANP/BNP) and PTX3. The pathological changes in the myocardial tissues were observed by hematoxylin and eosin (HE) and Masson stainings. The mRNA and protein expressions were detected by quantitative real-time reverse-transcription polymerase chain reaction (qPCR) and western blotting. Cardiomyocyte apoptosis was evaluated by TUNEL staining and DNA fragmentation test. Increased plasma concentrations of PTX3 were found in SHHF rats compared with SD rats, which was further enhanced by recombinant PTX3 protein. After injection with recombinant PTX3 protein, the heart function was improved in SHHF rats with the decreased systolic and diastolic BP, and the reduced plasma levels of ANP and BNP. Moreover, PTX3 improved the myocardial damage and interstitial fibrosis in SHHF rats with reduced cardiomyocyte apoptosis and decreased mRNA expressions of pro-inflammatory factors in myocardial tissues. PTX3 could decrease the BP and plasma levels of ANP and BNP in SHHF rats, as well as improve the inflammation, cardiomyocyte apoptosis, and pathological changes of myocardial tissues, suggesting it may be a useful intervention in the treatment of SHHF.
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http://dx.doi.org/10.1007/s12012-021-09671-0DOI Listing
October 2021

Increasing proportions of relapsing parasite species among imported malaria in China's Guangxi Province from Western and Central Africa.

Travel Med Infect Dis 2021 Sep-Oct;43:102130. Epub 2021 Jun 21.

Department of Pathogen Biology and Immunology, Kunming Medical University, Kunming, Yunnan Province, 650500, China. Electronic address:

Background: Travel-related malaria in non-endemic areas returning from endemic areas presents important challenges to diagnosis and treatment. Imported malaria to newly malaria-free countries poses further threats of malaria re-introduction and potential resurgence. For those traveling to places with high Plasmodium falciparum prevalence, prophylaxis against this parasite is recommended, whereas causal prophylaxis against relapsing malaria is often overlooked.

Methods: We analyzed a cluster of imported malaria among febrile patients in Shanglin County, Guangxi Province, China, who had recent travel histories to Western and Central Africa. Malaria was diagnosed by microscopy and subsequently confirmed by species- and subspecies-specific PCR. Plasmodium vivax was genotyped using a barcode consisting of 42 single nucleotide polymorphisms.

Results: Investigations of 344 PCR-confirmed malaria cases revealed that in addition to Plasmodium falciparum being the major parasite species, the relapsing parasites Plasmodium ovale and P. vivax accounted for ~40% of these imported cases. Of the 114 P. ovale infections, 65.8% and 34.2% were P. ovale curtisi and P. ovale wallikeri, respectively, with the two subspecies having a ~2:1 ratio in both Western and Central Africa. Phylogenetic analysis of 14 P. vivax isolates using a genetic barcode demonstrated that 11 formed a distinct clade from P. vivax populations from Eastern Africa.

Conclusion: This study provides support for active P. vivax transmission in areas with the predominant Duffy-negative blood group. With relapsing malaria making a substantial proportion of the imported malaria, causal prophylaxis should be advocated to travelers with a travel destination to Western and Central Africa.
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http://dx.doi.org/10.1016/j.tmaid.2021.102130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429216PMC
June 2021

Targeted delivery of regulatory macrophages to lymph nodes interferes with T cell priming by preventing the formation of stable immune synapses.

Cell Rep 2021 Jun;35(12):109273

Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany; Cluster of Excellence RESIST (EXC 2155), Hannover Medical School, 30625 Hannover, Germany; German Centre for Infection Research (DZIF), Partner Site Hannover-Braunschweig, 30625 Hannover, Germany. Electronic address:

Immunosuppressive myeloid cells are frequently induced in tumors and attenuate anti-tumor effector functions. In this study, we differentiate immunosuppressive regulatory macrophages (Mregs) from hematopoietic progenitors and test their potential to suppress adaptive immune responses in lymph nodes. Targeted delivery of Mregs to lymph nodes is facilitated by retroviral overexpression of the chemokine receptor CCR7 and intra-lymphatic cell application. Delivery of Mregs completely abolishes the priming of cognate CD8 cells and strongly reduces delayed-type hypersensitivity reactions. Mreg-mediated T cell suppression requires cell-cell contact-regulated nitric oxide production. Two-photon microscopy reveals that nitric oxide produced by Mregs reduces the interaction duration between dendritic cells and T cells. Exposure of activated T cells to nitric oxide strongly reduces their binding to ICAM-1, indicating that nitrosylation of proteins involved in cell adhesion affects synapse formation. Thus, this study identifies a mechanism of myeloid cell-mediated immune suppression and provides an approach for its therapeutic use.
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http://dx.doi.org/10.1016/j.celrep.2021.109273DOI Listing
June 2021

Hybrid MgCl/AlCl/Mg(TFSI) Electrolytes in DME Enabling High-Rate Rechargeable Mg Batteries.

ACS Appl Mater Interfaces 2021 Jul 22;13(26):30712-30721. Epub 2021 Jun 22.

Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Physical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China.

Rechargeable magnesium batteries (RMBs) are considered as one of the most promising next-generation secondary batteries due to their low cost, safety, dendrite-free nature, as well as high volumetric energy density. However, the lack of suitable cathode material and electrolyte is the greatest challenge facing practical RMBs. Herein, a hybrid electrolyte MgCl/AlCl/Mg(TFSI) (MACT) in dimethyl ether (DME) is developed and exhibits excellent electrochemical performance. The high ionic conductivity (6.82 mS cm) and unique solvation structure of [Mg(μ-Cl)(DME)] promote the fast Mg kinetics and favorable thermodynamics in hybrid Mg salts and DME electrolyte, accelerating mass transport and the charge transfer process. Therefore, the great rate capability can be realized both in symmetric Mg/Mg cell and in CuS/Mg full cell.
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http://dx.doi.org/10.1021/acsami.1c07567DOI Listing
July 2021

The clinical value of prenatal assessment of cervical length and placental thickness in pregnant women with placenta previa.

Am J Transl Res 2021 15;13(5):5308-5314. Epub 2021 May 15.

Department of Obstetrics and Gynecology, Fuzhou Maternal and Child Health Hospital (The Second People's Hospital of Fuzhou) Fuzhou, Jiangxi Province, China.

Objective: This study was designed to investigate the clinical value of prenatal assessment of cervical length (CL) and placental thickness (PT) in pregnancy outcome and prognosis of pregnant women with placenta previa.

Methods: Eighty pregnant women with placenta previa treated in our hospital were enrolled for prenatal assessment of CL and PT, and were grouped as CL ≤ 30 mm (n=32) and CL > 30 mm (n=48) groups and PT ≥ 10 mm (n=34) and PT < 10 mm (n=46) groups, respectively. The pregnancy and perinatal outcomes were compared in different groups. ROC curve of CL and PT on preterm delivery was drawn, and the diagnostic value of CL and PT in diagnosing preterm delivery was calculated.

Results: The pregnancy and perinatal outcomes of CL ≤ 30 mm group were significantly inferior to those of CL > 30 mm group ( < 0.05). The pregnancy and perinatal outcomes of PT ≥ 10 mm group were also significantly inferior to those of PT < 10 mm group ( < 0.05). PT and CL had good predictive values for preterm delivery ( < 0.05), with high diagnostic sensitivity, specificity and accuracy.

Conclusion: Prenatal assessment of CL and PT has practical clinical significance for pregnant women with placenta previa, which helps in assessing pregnancy and perinatal outcomes and is worthy of clinical application.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205795PMC
May 2021

Regulates Fatty Acid Metabolism to Ameliorate Liver Lipid Accumulation in NAFLD Mice.

Evid Based Complement Alternat Med 2021 19;2021:6689727. Epub 2021 Mar 19.

Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, No. 18, Chaowang Road, Xiacheng District, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China.

(DOF) is a traditional Chinese edible and officinal plant. Ultrafine DOF powder (DOFP) can regulate lipids and histopathology in the liver, but the underlying mechanisms of hepatic fatty acid (FA) metabolism, which is generally correlated with the development of nonalcoholic fatty liver disease (NAFLD), remain unclear. The purpose of the present study was to investigate whether DOFP treatment alters hepatic FA metabolism in NAFLD mice by using multidimensional mass spectrometry-based shotgun lipidomics (MDMS-SL) and analyse the underlying mechanisms. A 3-week DOFP treatment prevented lipid deposition and improved hepatic histopathology in NAFLD mice after withdrawal from the high-sucrose, high-fat (HSHF) diet, and it decreased triglyceride and FA content in the liver. Furthermore, the C16 : 0/C14 : 0 and C18 : 1/18 : 0 ratios in FAs were significantly decreased in the DOFP treatment group, and the C20 : 4/C20 : 3 and C22 : 4/C22 : 3 ratios were increased, and saturated FA was inhibited. Additionally, DOFP treatment significantly increased the content of two FA -oxidation-related proteins (carnitine palmitoyltransferase 1- and acyl-coenzyme A oxidase 1). It also decreased the content of a FA synthesis-related protein (fatty acid synthase), a FA desaturation-related protein (stearoyl-coenzyme A desaturase-1), and a FA uptake-related protein (fatty acid transport protein 2). Moreover, DOFP treatment improved dysregulated levels of major phospholipids in the livers of model mice. The results of this study confirm that DOFP treatment in NAFLD mice has liver recovery effects by regulating FA metabolism.
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http://dx.doi.org/10.1155/2021/6689727DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189787PMC
March 2021

Potential biomarkers for inherited thrombocytopenia 2 identified by plasma proteomics.

Platelets 2021 Jun 8:1-8. Epub 2021 Jun 8.

Laboratory of Radiation Biology, Department of Blood Transfusion, Laboratory Medicine Center, The Second Affiliated Hospital, Army Medical University, Chongqing, China.

Inherited thrombocytopenia 2 (THC2) is difficult to diagnose due to the lack of specific clinical characteristics and diagnostic methods. To identify potential plasma protein biomarkers for THC2, we collected the plasma samples from a THC2 family (9 THC2 and 15 non-THC2 members), enriched the medium and low abundant proteins using Proteominer and analyzed the protein profiles using the liquid chromatography-mass spectrometry in data independent acquisition mode. Initially, we detected 784 proteins in the plasma samples of this family and identified 27 up-regulated and 36 down-regulated in the THC2 group compared to the non-THC2 group (|log ratio| >1 and -value <0.05). To improve the predictive power, top eight dysregulated proteins (B7Z2B4, LTF, HP, ERN1, IGHV1-8, A0A0X9V9C4, VH6DJ, and D3JV41) were selected by an area under the curve-based random forest process to construct a clinical model. Multivariate analysis with random forest and support vector machine showed that the prediction model provided high discrimination ability for THC2 diagnosis (AUC: 1.000 and 0.967, respectively). The potential plasma protein biomarkers will be tested in more THC2 patients and other thrombocytopenia patients to further validate their specificity and sensitivity.
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http://dx.doi.org/10.1080/09537104.2021.1937594DOI Listing
June 2021

A novel flavonoid with antioxidant activity from (Thunb.) Juss.

Nat Prod Res 2021 Jun 4:1-7. Epub 2021 Jun 4.

School of Pharmaceutical Science, Liaoning University, Shenyang, Liaoning, P.R. China.

(Thunb.) Juss (), a perennial herb, is widely used as a medicinal plant in Chinese folk. This study aims to isolate and identify the chemical constituents from and evaluate their antioxidant activity. Normal silica column chromatography, ODS silica column chromatography, Sephadex LH-20 column chromatography and semi-preparative HPLC methods were used to obtain a new compound named 3-n-pentadecyl-4'-methoxyluteolin (1) and two known compounds including luteolin-7-O-β-D-glucuronide methyl ester (2) and apigenin-7-O-β-D-glucuronide methyl ester (3). The antioxidant activity of these compounds was determined by DPPH and ABTS methods and the IC50 values were calculated. The IC50 values of ABTS scavenging activity of 1, 2 and 3 were 12.99 ± 0.09 μM, 7.13 ± 0.07 μM and 5.15 ± 0.08 μM, respectively, and the IC50 values of DPPH scavenging activity of 1, 2 and 3 were 51.86 ± 0.41 μM, 23.95 ± 0.71 μM and 25.06 ± 0.65 μM, respectively. All the compounds exhibited good antioxidant activities in vitro.
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http://dx.doi.org/10.1080/14786419.2021.1935931DOI Listing
June 2021

Effects of Macroporous Resin Extract of Dendrobium officinale Leaves in Rats with Hyperuricemia Induced by Fructose and Potassium Oxonate.

Comb Chem High Throughput Screen 2021 May 28. Epub 2021 May 28.

Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, China.

Aim And Objective: Fructose, as a ubiquitous monosaccharide, can promote ATP consumption and elevate circulating uric acid (UA) levels. Our previous studies confirmed that the macroporous resin extract of Dendrobium officinale leaves (DoMRE) could reduce the UA level of rats with hyperuricemia induced by a high-purine diet. This study aimed to investigate whether DoMRE had a UA-lowering effect on rats with hyperuricemia caused by fructose combined with potassium oxonate, so as to further clarify the UA-lowering effect of DoMRE, and to explore the UA-lowering effect of DoMRE on both UA production and excretion.

Materials And Methods: Rats with hyperuricemia induced by fructose and potassium oxonate were administered with DoMRE and vehicle control, respectively, to compare the effects of the drugs. At the end of the experiment, the serum uric acid (SUA) and creatinine (Cr) levels were measured using an automatic biochemical analyzer, the activities of xanthine oxidase (XOD) were measured using an assay kit, and the protein expression of urate transporter 1 (URAT1), glucose transporter 9 (GLUT9), and ATP-binding cassette superfamily G member 2 (ABCG2) were assessed using immunohistochemical and western blot analyses. Hematoxylin and eosin staining was used to assess the histological changes in the kidney, liver, and intestine.

Results: Rats with hyperuricemia were induced by fructose and potassiumFructose and potassium induced hyperuricemia in rats. Meanwhile, the activities of XOD were markedly augmented, the expression of URAT1 and GLUT9 was promoted, and the expression of ABCG2 was reduced, which were conducive to the elevation of UA. However, exposure to DoMRE reversed these fructose- and potassium oxonate-induced negative alternations in rats. The activities of XOD were recovered to the normal level, reducing UA formation; the expression of URAT1, ABCG2, and GLUT9 returned to the normal level, resulting in an increase in renal urate excretion.

Conclusion: DoMRE reduces UA levels in rats with hyperuricemia induced by fructose combined with potassium oxonate by inhibiting XOD activity and regulating the expression of ABCG2, URAT1, and GLUT9. DoMRE is a potential therapeutic agent for treating hyperuricemia through inhibiting UA formation and promoting UA excretion.
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http://dx.doi.org/10.2174/1386207324666210528114345DOI Listing
May 2021

Pharmacological targeting of TNS3 with histone deacetylase inhibitor as a therapeutic strategy in esophageal squamous cell carcinoma.

Aging (Albany NY) 2021 05 28;13(11):15336-15352. Epub 2021 May 28.

Department of Gastroenterology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.

Histone acetylation which regulates about 2-10% of genes has been demonstrated to be involved in tumorigenesis of esophageal squamous cell carcinoma (ESCC). In this study, we investigated the treatment response of ESCC to selective histone deacetylase inhibitor (HDACi) LMK-235 and potential biomarker predicting the treatment sensitivity. We identified tensin-3 (TNS3) which was highly over-expressed in ESCC as one of the down-regulated genes in response to LMK-235 treatment. TNS3 was found positively correlated with the tumor malignancy and poor prognosis in the patients. Silencing significantly inhibited ESCC cell proliferation both and , sensitizing the treatment response to LMK-235. Our findings provide an insight into understanding the oncogenic role of TNS3 in ESCC and its clinical application for HDAC targeted therapy of ESCC.
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http://dx.doi.org/10.18632/aging.203091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8221360PMC
May 2021

Loop-mediated isothermal amplification (LAMP) assays targeting 18S ribosomal RNA genes for identifying P. vivax and P. ovale species and mitochondrial DNA for detecting the genus Plasmodium.

Parasit Vectors 2021 May 24;14(1):278. Epub 2021 May 24.

Department of Pathogen Biology and Immunology, Kunming Medical University, Kunming, 650500, Yunnan, People's Republic of China.

Background: Loop-mediated isothermal amplification (LAMP) has been widely used to diagnose various infectious diseases. Malaria is a globally distributed infectious disease attributed to parasites in the genus Plasmodium. It is known that persons infected with Plasmodium vivax and P. ovale are prone to clinical relapse of symptomatic blood-stage infections. LAMP has not previously been specifically evaluated for its diagnostic performance in detecting P. ovale in an epidemiological study, and no commercial LAMP or rapid diagnostic test (RDT) kits are available for specifically diagnosing infections with P. ovale.

Methods: An assay was designed to target a portion of mitochondrial DNA (mtDNA) among Plasmodium spp., the five human Plasmodium species and two other assays were designed to target the nuclear 18S ribosomal DNA gene (18S rDNA) of either P. vivax or P. ovale for differentiating the two species. The sensitivity of the assays was compared to that of nested PCR using defined concentrations of plasmids containing the target sequences and using limiting dilutions prepared from clinical isolates derived from Chinese workers who had become infected in Africa or near the Chinese border with Myanmar.

Results: The results showed that 10 copies of the mitochondrial target or 10 and 10 copies of 18S rDNA could be detected from Plasmodium spp., P. vivax and P. ovale, respectively. In 279 clinical samples, the malaria Pan mtDNA LAMP test performed well when compared with a nested PCR assay (95% confidence interval [CI] sensitivity 98.48-100%; specificity 90.75-100%). When diagnosing clinical cases of infection with P. vivax, the 18S rDNA assay demonstrated an even great sensitivity (95.85-100%) and specificity (98.1-100%). The same was true for clinical infections with P. ovale (sensitivity 90.76-99.96%; specificity 98.34-100%). Using plasmid-positive controls, the limits of detection of Malaria Pan, 18S rDNA P. vivax and 18S rDNA P. ovale LAMP were 100-, 100- and tenfold lower than those of PCR, respectively.

Conclusion: The novel LAMP assays can greatly aid the rapid, reliable and highly sensitive diagnosis of infections of Plasmodium spp. transmitted among people, including P. vivax and P. ovale, cases of which are most prone to clinical relapse.
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http://dx.doi.org/10.1186/s13071-021-04764-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147439PMC
May 2021

Beneficial effects of Dendrobium officinale on metabolic hypertensive rats by triggering the enteric-origin SCFA-GPCR43/41 pathway.

Food Funct 2021 Jun 18;12(12):5524-5538. Epub 2021 May 18.

Zhejiang University of Technology, Hangzhou, Zhejiang 310014, PR China.

Given the increasing global trend toward unhealthy lifestyles and dietary decisions, such as "over-consumption of alcohol, and high sugar and fat diets" (ACHSFDs), it is not surprising that metabolic hypertension (MH) is now the most common type of hypertension. There is an urgent, global need for effective measures for the prevention and treatment of MH. Improper diet leads to decreased short-chain fatty acid (SCFA) production in the gut, leading to decreased gastrointestinal function, metabolism, and blood pressure as a result of signaling through G-protein-coupled receptors (GPCRs), ultimately causing MH. Previous studies have suggested that Dendrobium officinale (DO) may improve gastrointestinal function, lower blood pressure, and regulate metabolic abnormalities, but it is not clear whether it acts on MH by increasing SCFA and, if so, how. In this research, it was observed that Dendrobium officinale ultrafine powder (DOFP) could lower blood pressure and improve lipid abnormalities in ACHSFD-induced MH model rats. Moreover, DOFP was found to improve the intestinal flora and increased the SCFA level in feces and serum, as well as increased the expressions of GPCR43/41 and eNOS and the nitric oxide (NO) level. An experiment on isolated aorta rings revealed that DOFP improved the vascular endothelial relaxation function in MH rats, and this effect could be blocked by the eNOS inhibitor l-NAME. These experimental results suggest that DOFP improved the intestinal flora and increased the production, transportation, and utilization of SCFA, activated the intestinal-vascular axis SCFA-GPCR43/41 pathway, improved vascular endothelial function, and finally lowered blood pressure in MH model rats. This research provides a new focus for the mechanism of the effect of DOFP against MH by triggering the enteric-origin SCFA-GPCR43/41 pathway.
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http://dx.doi.org/10.1039/d0fo02890hDOI Listing
June 2021

Ultralow-noise broadband source for interferometric fiber optic gyroscopesemploying a semiconductor optical amplifier.

Appl Opt 2021 Apr;60(11):3103-3107

A novel scheme of an ultralow relative intensity noise (RIN) broadband source module employing a double pumped backward (DPB) Er-doped superfluorescence fiber source (EDSFS) and a semiconductor optical amplifier for interferometric fiber optic gyroscopes (IFOGs) is proposed. With optimized parameters, the optimal twin-peak output profile of the source is obtained. The effective optical spectrum width of the source is 38.6 nm, and the output power is about 12.5 mW. Compared with the DPB EDSFS with a similar spectrum, the ultralow RIN broadband source proposed demonstrates a lower RIN of about 8.4 dB. A high-precision IFOG utilizing the ultralow RIN broadband source is set up, and the performance of the IFOG is experimentally studied. An angle random walk coefficient of 6.93×10/ is demonstrated, which is reduced by about 31.5% compared with the same IFOG system utilizing conventional DPB EDSFS with a similar spectrum profile. The ultralow RIN broadband source module proposed is quite feasible for high-precision IFOGs used in strategic-grade navigation systems and satellites.
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http://dx.doi.org/10.1364/AO.419969DOI Listing
April 2021

A zinc finger protein gene signature enables bladder cancer treatment stratification.

Aging (Albany NY) 2021 05 7;13(9):13023-13038. Epub 2021 May 7.

Beijing Chaoyang Hospital Affiliated Capital Medical University, Beijing 100020, China.

Bladder cancer (BC) is a commonly occurring malignant tumor affecting the urinary tract. Zinc finger proteins (ZNFs) constitute the largest transcription factor family in the human genome and are therefore attractive biomarker candidates for BC prognosis. In this study, we profiled the expression of ZNFs in The Cancer Genome Atlas (TCGA) BC cohort and developed a novel prognostic signature based on 7 ZNF-coding genes. After external validation of the model in the GSE48276 dataset, we integrated the 7-ZNF-gene signature with patient clinicopathological data to construct a nomogram that forecasted 1-, 2-, and 3-year OS with good predictive accuracy. We then accessed The Genomics of Drug Sensitivity in Cancer database to predict the therapeutic drug responses of signature-defined high- and low-risk BC patients in the TCGA cohort. Greater sensitivity to chemotherapy was revealed in the low-risk group. Finally, we conducted gene set enrichment analysis of the signature genes and established, by applying the ESTIMATE algorithm, distinct correlations between the two risk groups and the presence of stromal and immune cell types in the tumor microenvironment. By allowing effective risk stratification of BC patients, our novel ZNF gene signature may enable tailoring more intensive treatment for high-risk patients.
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http://dx.doi.org/10.18632/aging.202984DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8148496PMC
May 2021

GC-MS/MS method for determination and pharmacokinetics of sclareol in rat plasma after intravenous administration.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Apr 20;1173:122703. Epub 2021 Apr 20.

School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, People's Republic of China. Electronic address:

Sclareol, mainly isolated from Salvia officinalis, has a variety of pharmacological effects. In this work, a sensitive and rapid gas chromatography-tandem mass spectrometry (GC-MS/MS) method was first developed and validated for the determination and pharmacokinetics of sclareol in rat plasma. The chromatographic seperation of biosamples was performed with an HP-5MS column. Ethyl acetate was selected as the solvent to extract analytes from rat plasma. The multiple reaction monitoring transitions for sclareol and dehydrocostuslactone (Internal Standard, IS) were m/z 177 → 121 and m/z 230 → 173, respectively. The intra- and inter- precision, accuracy, matrix effect, recovery and stability meet the method requirements for biological sample analysis. The lowest limit of quantification (LLOQ) of the developed method for sclareol determination was 20 ng/mL. After intravenous administration (5.0 mg/kg) of sclareol to the rats, its drug clearance (CLz) and elimination half-life (t) was 2.7 ± 1.3 L/h/kg and 6.0 ± 4.6 h, respectively. The apparent volume of distribution (V) was 21.4 ± 12.9 L/kg, which indicated that sclareol was mainly distributed in extracellular fluid. Our results provided useful information for the further pharmacological investigation and preclinical studies of sclareol.
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http://dx.doi.org/10.1016/j.jchromb.2021.122703DOI Listing
April 2021

Gut bacterial communities across 12 Ensifera (Orthoptera) at different feeding habits and its prediction for the insect with contrasting feeding habits.

PLoS One 2021 26;16(4):e0250675. Epub 2021 Apr 26.

College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding, China.

Insect microbial symbioses play a critical role in insect lifecycle, and insect gut microbiome could be influenced by many factors. Studies have shown that host diet and taxonomy have a strong influence on insect gut microbial community. In this study, we performed sequencing of V3-V4 region of 16S rRNA gene to compare the composition and diversity of 12 Ensifera from 6 provinces of China. Moreover, the influences of feeding habits and taxonomic status of insects on their gut bacterial community were evaluated, which might provide reference for further application research. The results showed that Proteobacteria (45.66%), Firmicutes (34.25%) and Cyanobacteria (7.7%) were the predominant bacterial phyla in Ensifera. Moreover, the gut bacterial community composition of samples with different feeding habits was significantly different, which was irrespective of their taxa. The highest diversity of gut bacteria was found in the omnivorous Ensifera. Furthermore, common and unique bacteria with biomarkers were found based on the dietary characteristics of the samples. However, the bacterial community structure of the Ensifera samples was significantly different from that of Caelifera. Therefore, we concluded that feeding habits and taxonomic status jointly affect the gut bacterial community composition of the samples from Orthoptera. However, the influence of feeding habit dominates when taxonomy category below the suborder level. In addition, the dominant, common and unique bacterial community structure could be used to predict the contrastic feeding habits of insects belonging to Ensifera.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0250675PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075264PMC
April 2021
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