Publications by authors named "Yu Qian"

1,058 Publications

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MicroRNA-497 induced by Clonorchis sinensis enhances the TGF-β/Smad signaling pathway to promote hepatic fibrosis by targeting Smad7.

Parasit Vectors 2021 Sep 14;14(1):472. Epub 2021 Sep 14.

Jiangsu Key Laboratory of Immunity and Metabolism, Department of Pathogenic Biology and Immunology, Xuzhou Key Laboratory of Infection and Immunity, Xuzhou Medical University, Jiangsu, 221004, Xuzhou, People's Republic of China.

Background: Various stimuli, including Clonorchis sinensis infection, can cause liver fibrosis. Liver fibrosis is characterized by the activation of hepatic stellate cells (HSCs) with massive production of extracellular matrix (ECM). Our previous study showed that the TGF-β-induced Smad signaling pathway played a critical role in the activation of HSCs during liver fibrosis induced by worm infection; however, the mechanisms that modulate the TGF-β/Smad signaling pathway are still poorly understood. Accumulating evidence demonstrates that miRNAs act as an important regulator of activation of HSCs during liver fibrosis.

Methods: The target of miR-497 was determined by bioinformatics analysis combined with a dual-luciferase activity assay. LX-2 cells were transfected with miR-497 inhibitor and then stimulated with TGF-β or excretory/secretory products of C. sinensis (CsESPs), and activation of LX-2 was assessed using qPCR or western blot. In vivo, the mice treated with CCl were intravenously injected with a single dose of adeno-associated virus serotype 8 (AAV8) that overexpressed anti-miR-497 sequences or their scramble control for 6 weeks. Liver fibrosis and damage were assessed by hematoxylin and eosin (H&E) staining, Masson staining, and qPCR; the activation of the TGF-β/Smad signaling pathway was detected by qPCR or western blot.

Results: In the present study, the expression of miR-497 was increased in HSCs activated by TGF-β or ESPs of C. sinensis. We identified that Smad7 was the target of miR-497 using combined bioinformatics analysis with luciferase activity assays. Transfection of anti-miR-497 into HSCs upregulated the expression of Smad7, leading to a decrease in the level of p-Smad2/3 and subsequent suppression of the activation of HSCs induced by TGF-β or CsESPs. Furthermore, miR-497 inhibitor delivered by highly-hepatotropic (rAAV8) inhibited TGF-β/smads signaling pathway by targeting at Smad7 to ameliorate CCL4-induced liver fibrosis.

Conclusions: The present study demonstrates that miR-497 promotes liver fibrogenesis by targeting Smad7 to promote TGF-β/Smad signaling pathway transduction both in vivo and in vitro, which provides a promising therapeutic strategy using anti-miR-497 against liver fibrosis.
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http://dx.doi.org/10.1186/s13071-021-04972-3DOI Listing
September 2021

Comparison and optimization of various moving patient-based real-time quality control procedures for serum sodium.

J Clin Lab Anal 2021 Sep 14:e23985. Epub 2021 Sep 14.

Department of Laboratory Medicine, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.

Background: Patient-based real-time quality control (PBRTQC) is a valuable tool for monitoring the performance of testing processes. We aimed to compare and optimize various PBRTQC procedures for serum sodium.

Methods: In a computer simulation, artificial errors were added to 680,000 real patients' results. The characteristics of error detection of various algorithms-moving average, moving median, moving SD and moving proportion of normal results including different control limits (CLs)-were assessed on their ability to detect critical errors early.

Results: The moving average and moving median were sensitive to system error, and the moving SD tended to detect random error. P (moving proportion of normal results, CLs based on mean and SD of proportion of normal results) demonstrated excellent performance for both system error and random error. The increase of block sizes (N) leads to the delay of error detection and the decrease of false rejection, except for QC procedures with minimum and maximum as CLs. CLs calculation with "0.1% false alarm rate" had more effective performance than that set false alarm to zero (minimum and maximum as CLs). The impact of truncation on QC performance depended on truncation limits, algorithms and the types of error. The significant improvement in QC performance due to truncation was only found in moving SD.

Conclusion: "P ,N = 50, without truncation" and "moving SD, N = 25, set 0.1% false alarm as CLs and set 1% outliers exclusion as truncation limits" were recommended as the optimized procedures for serum sodium to monitor system error and random error, respectively.
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http://dx.doi.org/10.1002/jcla.23985DOI Listing
September 2021

Dual-Functional Surfaces Based on an Antifouling Polymer and a Natural Antibiofilm Molecule: Prevention of Biofilm Formation without Using Biocides.

ACS Appl Mater Interfaces 2021 Sep 14. Epub 2021 Sep 14.

State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, P. R. China.

Pathogenic biofilms formed on the surfaces of implantable medical devices and materials pose an urgent global healthcare problem. Although conventional antibacterial surfaces based on bacteria-repelling or bacteria-killing strategies can delay biofilm formation to some extent, they usually fail in long-term applications, and it remains challenging to eradicate recalcitrant biofilms once they are established and mature. From the viewpoint of microbiology, a promising strategy may be to target the middle stage of biofilm formation including the main biological processes involved in biofilm development. In this work, a dual-functional antibiofilm surface is developed based on copolymer brushes of 2-hydroxyethyl methacrylate (HEMA) and 3-(acrylamido)phenylboronic acid (APBA), with quercetin (Qe, a natural antibiofilm molecule) incorporated via acid-responsive boronate ester bonds. Due to the antifouling properties of the hydrophilic poly(HEMA) component, the resulting surface is able to suppress bacterial adhesion and aggregation in the early stages of contact. A few bacteria are eventually able to break through the protection of the anti-adhesion layer leading to bacterial colonization. In response to the resulting decrease in the pH of the microenvironment, the surface could then release Qe to interfere with the microbiological processes related to biofilm formation. Compared to bactericidal and anti-adhesive surfaces, this dual-functional surface showed significantly improved antibiofilm performance to prevent biofilm formation involving both Gram-negative and Gram-positive for up to 3 days. In addition, both the copolymer and Qe are negligibly cytotoxic, thereby avoiding possible harmful effects on adjacent normal cells and the risk of bacterial resistance. This dual-functional design approach addresses the different stages of biofilm formation, and (in accordance with the growth process of the biofilm) allows sequential activation of the functions without compromising the viability of adjacent normal cells. A simple and reliable solution may thus be provided to the problems associated with biofilms on surfaces in various biomedical applications.
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http://dx.doi.org/10.1021/acsami.1c10747DOI Listing
September 2021

A stable tetrazagallole and its radical anion dimer.

Chem Commun (Camb) 2021 Sep 14;57(73):9268-9271. Epub 2021 Sep 14.

College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China.

The reaction of the carbazole ligand supported Ga(I) compound LGa(THF) (3) and 1-azido-4-(-butyl)benzene (ArN) afforded the first stable tetrazagallole LGaNAr (4) bearing a three-coordinate Ga atom. Reduction of 4 with elemental potassium resulted in the radical dimer {[K(18-c-6)][4]˙}, featuring a strong antiferromagnetic interaction between the spin centers.
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http://dx.doi.org/10.1039/d1cc03448kDOI Listing
September 2021

Assessment of PD-L1 Expression on Circulating Tumor Cells for Predicting Clinical Outcomes in Cancer Patients Receiving PD-1/PD-L1 Blockade Therapies.

Oncologist 2021 Sep 13. Epub 2021 Sep 13.

Department of Gastrointestinal Oncology, The Fifth Medical Center, Chinese PLA General Hospital, Beijing, China.

Background: PD-1/PD-L1 blockade immunotherapies have changed the landscape of cancer therapy. However, the main limitation of these therapies is the lack of definitively predictive biomarkers to predict treatment response. Whether PD-L1 expression on circulating tumor cells (CTCs) is associated with the clinical outcomes of immunotherapy remains to be extensively investigated.

Methods: 155 patients with different advanced cancers were enrolled in this study and treated with anti-PD-1/PD-L1 monoclonal antibodies (mAbs). Using the [email protected] method, CTCs were isolated and enumerated. The PD-L1 expression levels were analyzed by an immunofluorescence assay for semiquantitative assessment with four categories (negative, low, medium and high).

Results: Prior to immunotherapy, 81.93% (127/155) of patients had PD-L1-positive CTCs, and 71.61% (111/155) had at least one PD-L1-high CTC. The group with PD-L1-positive CTCs had a higher disease control rate (DCR) (71.56%, 91/127), with a DCR of only 39.29% (11/28) for the remaining individuals (P=0.001). The objective response rate (ORR) and DCR in PD-L1-high patients were higher than those in the other patients (32.44% vs 13.64%, P=0.018 and 75.68% vs 40.91%, P<0.0001, respectively). The reduction in the counts and ratios of PD-L1-positive CTCs and PD-L1-high CTCs reflected a beneficial response to PD-1/PD-L1 inhibitors. Furthermore, patients with PD-L1-high CTCs had significantly longer progression-free survival (PFS) (4.9 vs 2.2 months, P<0.0001) and overall survival (OS) (16.1 vs 9.0 months, P=0.0235) than those without PD-L1-high CTCs.Conclusions The PD-L1 level on CTCs may serve as a clinically actionable biomarker for immunotherapy, and its dynamic changes could predict the therapeutic response.

Implications For Practice: This study was designed to investigate the role of PD-L1 expression on circulating tumor cells in predicting and monitoring response to PD-1/PD-L1 blockade immunotherapies in patients with advanced cancer. The results of the study showed that PD-L1-high expression CTCs were both a predictive biomarker and a prognostic factor in patients with advanced cancer treated with anti-PD-1/PD-L1 mAbs. These observations suggest that PD-L1 level on CTCs is a potential clinical biomarker for immunotherapy.
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http://dx.doi.org/10.1002/onco.13981DOI Listing
September 2021

The boosting of microwave roasting technology on the desulfurization of phosphate rock.

Environ Sci Pollut Res Int 2021 Sep 10. Epub 2021 Sep 10.

School of Chemistry and Environment, Yunnan Minzu University, Kunming, Yunnan, 650500, People's Republic of China.

A green and-easy to operate method, the microwave technology, was developed to promote the desulfurization process of phosphate rock, systematically investigates the strengthening effect of microwave, and uses XRD, BET, SEM, XRF, ICP, and EDS to characterize the reactants. The results show that the main reason for the desulfurization efficiency is improved by microwave heating under microwave conditions, different thermal stress phosphate rock materials lead to the destruction of each microstructure, and a specific surface area increased 40.25% phosphate rock. In addition, after microwave irradiation, the pore size of the phosphate rock at 2-5 nm is significantly increased, and the number of mesopores is significantly increased, thereby increasing the desulfurization efficiency of the phosphate rock. By investigating the effects of temperature, oxygen content, flow rate, and solid-liquid ratio on desulfurization efficiency, the paper concludes that the optimal conditions for desulfurization of phosphate rock after microwave irradiation are C(SO) is 2500 mg·m, temperature is 40 °C, φ(O) is 5%, solid-liquid ratio is 3.5 g:200 ml, and flue gas flow is 500 ml·min.
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http://dx.doi.org/10.1007/s11356-021-15731-3DOI Listing
September 2021

Sulperazon-induced acute reactive thrombocytopenia during treatment of systemic lupus erythematosus: a case report.

Eur J Hosp Pharm 2021 09 8. Epub 2021 Sep 8.

Department of Laboratory Medicine, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China

The purpose of this study is to report a patient who developed acute reactive thrombocytopenia while undergoing treatment with sulperazon for systemic lupus erythematosus (SLE). Sulperazon is a broad-spectrum antibiotic that can act against a wide range of microorganisms, but rarely causes severe thrombocytopenic events. We describe a 62-year-old man with new-onset acute reactive thrombocytopenia who experienced a precipitous fall in the platelet count from 168×10/L to 1×10/L within 29 hours after exposure to sulperazon. Sulperazon was immediately discontinued followed by administration of intravenous immunoglobulin for six consecutive days. The platelet count eventually recovered and petechiae at the injection sites improved. No complications secondary to acute reactive thrombocytopenia were observed except petechiae.
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http://dx.doi.org/10.1136/ejhpharm-2021-002999DOI Listing
September 2021

A simple quinoline-thiophene Schiff base turn-off chemosensor for Hg detection: spectroscopy, sensing properties and applications.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Aug 28;264:120338. Epub 2021 Aug 28.

School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address:

A new Schiff base probe (QT) consisting of 8-aminoquinoline (Q) and thiophene-2-carboxaldehyde (T) moieties has been synthesized. QT undergoes chelation-enhanced fluorescence quenching when exposed to Hg due to coordination by the sulfur and nitrogen atoms of QT thus forming a facile "turn-off" sensor. The formation of the chelation complex was confirmed by UV-visible absorption and emission spectral measurements, H NMR titration and density functional theory calculations. These studies revealed that the probe exhibits high selectivity and sensitivity towards Hg in the presence of other common metal ions. A low detection limit of 23.4 nM was determined and a Job plot confirmed a 2:1 stoichiometry between QT and Hg. The potential utility of QT as a sensor for Hg ions in human HeLa cells was determined by confocal fluorescence microscopy, and its suitability for use in the field with environmental samples was tested with Whatman filter paper strips.
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http://dx.doi.org/10.1016/j.saa.2021.120338DOI Listing
August 2021

Disorganized resting-state functional connectivity between the dorsal attention network and intrinsic networks in Parkinson's disease with freezing of gait.

Eur J Neurosci 2021 Sep 3. Epub 2021 Sep 3.

Department of Neurology, the First Affiliated Hospital, Chongqing Medical University, Chongqing, China.

Freezing of gait (FOG) is a common and complex manifestation of Parkinson's disease (PD) and is associated with impairment of attention. The purpose of this study was to evaluate the functional network connectivity (FNc) changes between the dorsal attention network (DAN) and the other seven intrinsic networks relevant to attention, visual-spatial, executive and motor functions in PD with or without FOG. Forty-three idiopathic PD patients (21 with FOG [FOG+] versus 22 without FOG [FOG-]) and 18 healthy controls (HC) were recruited in this study. The data-driven independent component analysis (ICA) method was used to extract and analyze the above-mentioned resting-state networks (RSNs). Compared with FOG-, FOG+ displayed decreased positive connectivity between the DAN and medial visual network (mVN) and sensory-motor network (SMN) and increased negative connectivity between the DAN and default mode network (DMN). The within-network connectivity in the SMN and visual networks were decreased, whereas the connectivity within DMN was increased significantly in FOG+. Correlation analysis showed that the clock drawing test (CDT) scores were positively correlated with the functional connectivity of mVN (r = 0.573, p = 0.008) and lateral visual network (lVN) (r = 0.510, p = 0.022), the Timed Up and Go Test (TUG) duration were negatively correlated with the connectivity of SMN (r = -0.629, p = 0.003), and the Frontal Assessment Battery (FAB) scores were negatively correlated with the connectivity of DMN in FOG+. Functional connectivity was changed in multiple intra-networks in patients with FOG. Inordinate inter-network connectivity between the DAN and other intrinsic networks may partly contribute to the mechanism of freezing.
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http://dx.doi.org/10.1111/ejn.15439DOI Listing
September 2021

Critical loads of headwater streams in China using SSWC model modified by comprehensive F-factor.

Sci Total Environ 2021 Aug 24;802:149780. Epub 2021 Aug 24.

State Key Laboratory of Environmental Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, PR China; State Environmental Protection Key Laboratory of Sources and Control of Air Pollution Complex, Beijing 100084, PR China. Electronic address:

In order to evaluate the potential risk of surface water acidification in regions with historically-elevated acid deposition and to measure the recovery of such ecosystems after policy changes, critical loads and their exceedances were estimated for 349 headwater streams across China using a modified SSWC model. Such a model considered the acid-neutralizing capacity derived from high base cation deposition and the robust retention of sulfate and nitrate. Results indicated that China's streams had higher critical loads (averaged at 4.7 keq·ha·yr) and were less sensitive to acid deposition as compared to Europe and North America. The proportion of surveyed streams with acid deposition exceeded critical load decreased from 40.4% in 2005 to 29.5% in 2018, indicating a significant decrease in risk of surface water acidification, and thus a benefit from the emission abatement in recent years. Nonetheless, a relatively high risk of acidification still existed in southeast China with lower critical loads and most critical load exceedances. More efforts should be put into implementing emission control policies in the future.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149780DOI Listing
August 2021

, Driving the Microglial Polarization, Has a Sensitivity Profile After Subarachnoid Hemorrhage.

Front Cell Dev Biol 2021 11;9:693342. Epub 2021 Aug 11.

Department of Neurosurgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

Increasing evidence suggests that triggering receptor expressed on myeloid cells 2 () is implicated in the pathophysiology of neuroinflammation. The aim here was to investigate the neuroprotective role of and its regulatory mechanism after subarachnoid hemorrhage (SAH). siRNA was administered to measure the detrimental role of in mediating microglial polarization and after experimental SAH. The relationship between Toll-like receptor 4 () signaling and was further explored. The soluble from the cerebrospinal fluid (CSF) of patients with SAH was detected. The results showed that mainly located in the microglia and presented a markedly delayed elevation after SAH. knockdown triggered increased pro-inflammatory productions, aggravated microglial activities, and further exacerbated neurological dysfunction after SAH. Significantly, knockout increased the expression of , accompanied by ameliorated neuroinflammation and improved neurological function. Corresponding to different clinical Hunt-Hess grades, obviously enhanced accumulation of soluble was detected in the CSF of patients with SAH. played a pivotal role in mediating microglial polarization after SAH, and the neuroprotective effect of might be potentially suppressed by the hyperactive in the early phase of SAH. Pharmacological targeting of may be a promising strategy for SAH therapy.
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http://dx.doi.org/10.3389/fcell.2021.693342DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386127PMC
August 2021

Impact of Surgical Approach on Surgical Resection Quality in Mid- and Low Rectal Cancer, A Bayesian Network Meta-Analysis.

Front Oncol 2021 11;11:699200. Epub 2021 Aug 11.

Department of Colorectal Surgery, Union Hospital, Fujian Medical University, Fuzhou, China.

Aim: To evaluate the evidence concerning the quality of surgical resection in laparoscopic (LapTME), robotic (RobTME) and transanal (TaTME) total mesorectal excision for mid-/low rectal cancer.

Methods: A systematic literature search of the PubMed, EMBASE and Cochrane Central Register of Controlled Trials databases was performed. A Bayesian network meta-analysis was utilized to compare surgical resection involved in these 3 surgical techniques by using ADDIS software. Rates of positive circumferential resection margins (CRMs) were the primary endpoint.

Results: A total of 34 articles, 2 randomized clinical trials (RCTs) and 32 non-RCTs, were included in this meta-analysis. Pooled data showed CRM positivity in 114 of 1763 LapTME procedures (6.5%), 54 of 1051 RobTME procedures (5.1%) and 60 of 1276 TaTME procedures (4.7%). There was no statistically significant difference among these 3 surgical approaches in terms of CRM involvement rates and all other surgical resection quality outcomes. The incomplete mesorectal excision rates were 9.6% (69/720) in the LapTME group, 1.9% (11/584) in the RobTME group and 5.6% (45/797) in the TaTME group. Pooled network analysis observed a higher but not statistically significant risk of incomplete mesorectum when comparing both LapTME with RobTME (OR = 1.99; 95% CI = 0.48-11.17) and LapTME with TaTME (OR = 1.90; 95% CI = 0.99-5.25). By comparison, RobTME was most likely to be ranked the best or second best in terms of CRM involvement, complete mesorectal excision, rate of distal resection margin (DRM) involvement and length of DRMs. In addition, RobTME achieved a greater mean tumor distance to the CRM than TaTME. It is worth noting that TaTME was most likely to be ranked the worst in terms of CRM involvement for intersphincteric resection of low rectal cancer.

Conclusion: Overall, RobTME was most likely to be ranked the best in terms of the quality of surgical resection for the treatment of mid-/low rectal cancer. TaTME should be performed with caution in the treatment of low rectal cancer.
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http://dx.doi.org/10.3389/fonc.2021.699200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385749PMC
August 2021

Strategy of Design toward First-In-Class Imaging Agents for Simultaneously Differentiating Glioma Boundary and Grades.

ACS Sens 2021 Aug 27. Epub 2021 Aug 27.

College of Pharmaceutical Sciences, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China.

The extent of resection and tumor grade are two predominant prognostic factors for glioma. Fluorescent imaging is promising to facilitate accurate resection and simultaneous tumor grading. However, no probe fulfilling this task has been reported. Herein, we proposed a strategy of design toward first-in-class fluorescent probes for simultaneously differentiating glioma boundary and grades. By bioinformatics analysis in combination with experimental validation, platelet-derived growth factor receptor β (PDGFRβ) was revealed as a promising biomarker for glioma imaging and grading. Then, fluorogenic probe was designed, guided by the structure-activity relationship study. Finally, the probe was demonstrated to stain glioma cells and tissues in the mice orthotopic glioma model with high selectivity over normal brain cells or tissues. Meanwhile, experiments using patient-derived samples indicated that the fluorescence was significantly positively correlated with the tumor grades. This result highlighted the feasibility of the three-step probe design strategy and suggested as a promising probe for simultaneously differentiating glioma boundary and grades, showing prospects of clinical translation.
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http://dx.doi.org/10.1021/acssensors.1c01168DOI Listing
August 2021

Higher Handgrip Strength Is Linked to Better Cognitive Performance in Chinese Adults with Hypertension.

Brain Sci 2021 Jul 25;11(8). Epub 2021 Jul 25.

Institute of KEEP Collaborative Innovation, Shenzhen University, Shenzhen 518060, China.

Objective: There is growing evidence that in adults, higher levels of handgrip strength (HGS) are linked to better cognitive performance. However, the relationship between HGS and cognitive performance has not been sufficiently investigated in special cohorts, such as individuals with hypertension who have an intrinsically higher risk of cognitive decline. Thus, the purpose of this study was to examine the relationship between HGS and cognitive performance in adults with hypertension using data from the Global Ageing and Adult Health Survey (SAGE).

Methods: A total of 4486 Chinese adults with hypertension from the SAGE were included in this study. Absolute handgrip strength (aHGS in kilograms) was measured using a handheld electronic dynamometer, and cognitive performance was assessed in the domains of short-term memory, delayed memory, and language ability. Multiple linear regression models were fitted to examine the association between relative handgrip strength (rHGS; aHGS divided by body mass index) and measures of cognitive performance.

Results: Overall, higher levels of rHGS were associated with higher scores in short-term memory (β = 0.20) and language (β = 0.63) compared with the lowest tertiles of rHGS. In male participants, higher HGS was associated with higher scores in short-term memory (β = 0.31), language (β = 0.64), and delayed memory (β = 0.22). There were no associations between rHGS and cognitive performance measures in females.

Conclusion: We observed that a higher level of rHGS was associated with better cognitive performance among hypertensive male individuals. Further studies are needed to investigate the neurobiological mechanisms, including sex-specific differences driving the relationship between measures of HGS and cognitive performance in individuals with hypertension.
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http://dx.doi.org/10.3390/brainsci11080985DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8391417PMC
July 2021

Reply to "Empiric Versus Targeted Transarterial Embolization for Upper Gastrointestinal Bleeding: No Need for Randomized Controlled Trial".

AJR Am J Roentgenol 2021 Aug 25:1-2. Epub 2021 Aug 25.

University of Chicago Medical Center, Chicago, IL.

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http://dx.doi.org/10.2214/AJR.21.26111DOI Listing
August 2021

A study of the efficacy and tolerability of capecitabine and lobaplatin in advanced HER-2 negative breast cancer patients.

Ann Transl Med 2021 Jul;9(14):1151

Department of Chemotherapy, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, China.

Background: This study sought to examine the efficacy and adverse reactions of capecitabine and lobaplatin in the treatment of metastatic human epidermal growth factor receptor 2 (HER-2) negative breast cancer (BC).

Methods: This retrospective study examined 45 patients diagnosed with advanced HER-2 negative BC. Patients were enrolled in this study from November 2015 to June 2019. The patients received capecitabine and lobaplatin combination therapy. The therapeutic efficacy and side effects were evaluated after at least 2 cycles of treatment.

Results: Therapeutic efficacy and adverse reactions were evaluated in 38 patients, comprising 12 cases of partial response (PR), 19 cases of stable disease (SD), and 7 cases of progressive disease (PD). Among these, 3 patients required treatment delays or dose reductions for subsequent cycles, and 2 patients discontinued treatment. The overall response rate (ORR) was 31.58% and the disease control rate (DCR) was 81.58%. The ORR and DCR for hormone receptor positive, HER-2 negative (HR+/HER-2-) and triple negative breast cancer (TNBC) patients were 31.82% and 31.25%, and 86.36% and 75%, respectively. The median progression free survival (PFS) was 8 months, 6 months, and 6 months in patients receiving the therapeutics as a first-line, second-line, or third-line and beyond treatment, respectively. The main side effects were myelosuppression, including granulocytopenia, thrombocytopenia, and anemia. Among patients with grade 1 side effects or above, 28 patients (73.68%) had myelosuppression, and 13 patients (34.21%) had gastrointestinal reactions. Further, we investigated the association between side effects and clinical outcomes, and found that PFS was increased in patients with myelosuppression and gastrointestinal reactions.

Conclusions: Capecitabine and lobaplatin combination therapy was effective and well tolerated among patients with advanced HER-2 negative BC.
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http://dx.doi.org/10.21037/atm-21-2702DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350665PMC
July 2021

Insomnia and other sleep-related problems during the remission period of the COVID-19 pandemic: A large-scale survey among college students in China.

Psychiatry Res 2021 10 3;304:114153. Epub 2021 Aug 3.

Center for Public Health Initiatives, University of Pennsylvania, Philadelphia, PA, USA.

This study aimed to evaluate the sleep-related problems and predictors of probable clinical insomnia among college students during the COVID-19 remission period in China. 146,102 college students from 22 colleges/universities in Guangdong province participated in this study from 1th to 15th June, 2020. Self-administered questionnaires were used to assess demographic characteristics. Sleep-related problems, depression and anxiety symptoms were measured by Youth Self-Rating Insomnia Scale, Patient Health Questionnaire-9 and Generalized Anxiety Disorder Scale-7, respectively. The prevalence of difficulty in initiating sleep, difficulty in maintaining sleep, early morning awakening, sleep insufficiency, unrefreshing sleep and daytime functioning impairment were 7.2%, 3.4%, 3.5%, 9.6%, 14.6%, and 7.6%, respectively. 16.9% students had varying degrees of insomnia and 6.3% were considered as displaying probable clinical insomnia. Moreover, being urban residents, having a history of physical or mental illness, and probable clinical depression or anxiety were significant risk factors of probable clinical insomnia, while college senior degree and 7-8 hours' sleep duration per day was the protective factor for probable clinical insomnia. Unrefreshing sleep was the most prominent sleep problem among college students during COVID-19 remission in China. Good sleep hygiene practices are strongly suggested to develop in the time of prolonged home isolation.
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http://dx.doi.org/10.1016/j.psychres.2021.114153DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8424256PMC
October 2021

Deep Symmetric Adaptation Network for Cross-modality Medical Image Segmentation.

IEEE Trans Med Imaging 2021 Aug 16;PP. Epub 2021 Aug 16.

Unsupervised domain adaptation (UDA) methods have shown their promising performance in the cross-modality medical image segmentation tasks. These typical methods usually utilize a translation network to transform images from the source domain to target domain or train the pixel-level classifier merely using translated source images and original target images. However, when there exists a large domain shift between source and target domains, we argue that this asymmetric structure, to some extent, could not fully eliminate the domain gap. In this paper, we present a novel deep symmetric architecture of UDA for medical image segmentation, which consists of a segmentation sub-network, and two symmetric source and target domain translation sub-networks. To be specific, based on two translation sub-networks, we introduce a bidirectional alignment scheme via a shared encoder and two private decoders to simultaneously align features 1) from source to target domain and 2) from target to source domain, which is able to effectively mitigate the discrepancy between domains. Furthermore, for the segmentation sub-network, we train a pixel-level classifier using not only original target images and translated source images, but also original source images and translated target images, which could sufficiently leverage the semantic information from the images with different styles. Extensive experiments demonstrate that our method has remarkable advantages compared to the state-of-the-art methods in three segmentation tasks, i.e., cross-modality cardiac, BraTS, and abdominal multi-organ segmentation.
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http://dx.doi.org/10.1109/TMI.2021.3105046DOI Listing
August 2021

Pneumocystis jiroveci pneumonia with cytomegalovirus infection diagnosed by metagenomic next-generation sequencing in a patient with nephrotic syndrome: A case report.

Medicine (Baltimore) 2021 Aug;100(31):e26842

Respiratory Department, Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University, Hangzhou, China.

Introduction: Opportunistic infection with multiple pathogens currently has become less uncommon since the application of immunosuppressant or corticosteroid in non- Human immunodeficiency virus patients. However, the clinical diagnosis of the co-infection remains difficult since the uncertainty and deficiency of the microbiologic testing methods.

Patient Concerns: A 66-year-old male patient was admitted to our hospital with chest stuffiness, shortness of breath and elevated body temperature.

Diagnosis: He was diagnosed with the co-infection of Pneumocystis jiroveci and cytomegalovirus by metagenomic next-generation sequencing of bronchoalveolar lavage fluid after bronchoscopy.

Interventions: The patient was empirically treated with broad-spectrum antibiotics, trimethoprim/ sulfamethoxazole and ganciclovir in the beginning of the admission.

Outcomes: The condition of this patient was not improved even with the intervention at the early stage of the disease. His family requested discharge after 24 inpatient days.

Lessons: This case highlights the application of metagenomic next-generation sequencing in the clinical diagnosis of pulmonary co-infection. Suitable prophylaxis, necessary clinical awareness and accurate diagnosis are indispensable for immunocompromised patients with pulmonary infection.
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http://dx.doi.org/10.1097/MD.0000000000026842DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8341323PMC
August 2021

A CPD photolyase gene PnPHR1 from Antarctic moss Pohlia nutans is involved in the resistance to UV-B radiation and salinity stress.

Plant Physiol Biochem 2021 Aug 6;167:235-244. Epub 2021 Aug 6.

National Glycoengineering Research Center and School of Life Science, Shandong University, Qingdao, 266237, China. Electronic address:

In Antarctic continent, the organisms are exposed to high ultraviolet (UV) radiation because of damaged stratospheric ozone. UV causes DNA lesions due to the accumulation of photoproducts. Photolyase can repair UV-damaged DNA in a light-dependent process by electron transfer mechanism. Here, we isolated a CPD photolyase gene PnPHR1 from Antarctic moss Pohlia nutans, which encodes a protein of theoretical molecular weight of 69.1 KDa. The expression level of PnPHR1 was increased by UV-B irradiation. Enzyme activity assay in vitro showed that PnPHR1 exhibited photoreactivation activity, which can repair CPD photoproducts in a light-dependent manner. The complementation assay of repair-deficient E. coli strain SY2 demonstrated that PnPHR1 gene enhanced the survival rate of SY2 strain after UV-B radiation. Additionally, overexpression of PnPHR1 enhanced the Arabidopsis resistance to UV-B radiation and salinity stress, which also conferred plant tolerance to oxidative stress by decreasing ROS production and increasing ROS clearance. Our work shows that PnPHR1 encodes an active CPD photolyase, which may participate in the adaptation of P. nutans to polar environments.
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http://dx.doi.org/10.1016/j.plaphy.2021.08.005DOI Listing
August 2021

New Structural Economic Analysis of Anti-COVID-19 Pandemic Model of BEST Region.

Int J Environ Res Public Health 2021 07 23;18(15). Epub 2021 Jul 23.

School of Finance & Economics, Jiangsu University, Zhenjiang 212013, China.

The successful anti-COVID-19 pandemic model of BEST region (Beijing-Seoul-Tokyo) includes China, Japan and South Korea, which benefit from its well-functioning organizational ecosystem and specific anti-COVID-19 pandemic strategies. Under the premise of an efficient market, the capable organizations of China, Japan and South Korea will play the dynamic function of coordination and organic connection. They will also help improve the governance efficiency of facilitating state in different stages of fighting against the pandemic. This article follows the analytical logic of the new structural economics, taking the factor endowment and its structure as the starting point for the analysis, through the comparative advantage operation mode determined by the market, and based on the collaborative anti-COVID-19 pandemic perspective of the government, the market and various social organizations, to build a framework for the facilitating state-efficient market-capable organization. The key to the success of the anti-COVID-19 pandemic method in China, Japan and South Korea is organically coordinated between government, market and organizations. Based on the effective promotion of micro-organizations, governments organize resource integration and implement macro-control of the market. A dynamic balance between economic governance and pandemic prevention and control has been achieved by optimizing the endowment structure of resources, improving infrastructure and reducing system costs.
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http://dx.doi.org/10.3390/ijerph18157822DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8345508PMC
July 2021

Episodic Memory Encoding and Retrieval in Face-Name Paired Paradigm: An NIRS Study.

Brain Sci 2021 Jul 19;11(7). Epub 2021 Jul 19.

Exercise Psychophysiology Laboratory, Institute of KEEP Collaborative Innovation, School of Psychology, Shenzhen University, Shenzhen 518060, China.

Background: Episodic memory (EM) is particularly sensitive to pathological conditions and aging. In a neurocognitive context, the paired-associate learning (PAL) paradigm, which requires participants to learn and recall associations between stimuli, has been used to measure EM. The present study aimed to explore whether functional near-infrared spectroscopy (NIRS) can be employed to determine cortical activity underlying encoding and retrieval. Moreover, we examined whether and how different aspects of task (i.e., novelty, difficulty) affects those cortical activities.

Methods: Twenty-two male college students (age: M = 20.55, SD = 1.62) underwent a face-name PAL paradigm under 40-channel NIRS covering fronto-parietal and middle occipital regions.

Results: A decreased activity during encoding in a broad network encompassing the bilateral frontal cortex (Brodmann areas 9, 11, 45, and 46) was observed during the encoding, while an increased activity in the left orbitofrontal cortex (Brodmann area 11) was observed during the retrieval. Increased HbO concentration in the superior parietal cortices and decreased HbO concentration in the inferior parietal cortices were observed during encoding while dominant activation of left PFC was found during retrieval only. Higher task difficulty was associated with greater neural activity in the bilateral prefrontal cortex and higher task novelty was associated with greater activation in occipital regions.

Conclusion: Combining the PAL paradigm with NIRS provided the means to differentiate neural activity characterising encoding and retrieval. Therefore, the NIRS may have the potential to complete EM assessments in clinical settings.
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http://dx.doi.org/10.3390/brainsci11070951DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8305286PMC
July 2021

Regression-Adjusted Real-Time Quality Control.

Clin Chem 2021 Aug 6. Epub 2021 Aug 6.

Department of Laboratory Medicine, Zhongshan Hospital, Fudan University.

Background: Patient-based real-time quality control (PBRTQC) has gained increasing attention in the field of clinical laboratory management in recent years. Despite the many upsides that PBRTQC brings to the laboratory management system, it has been questioned for its performance and practical applicability for some analytes. This study introduces an extended method, regression-adjusted real-time quality control (RARTQC), to improve the performance of real-time quality control protocols.

Methods: In contrast to the PBRTQC, RARTQC has an additional regression adjustment step before using a common statistical process control algorithm, such as the moving average, to decide whether an analytical error exists. We used all patient test results of 4 analytes in 2019 from Zhongshan Hospital, Fudan University, to compare the performance of the 2 frameworks. Three types of analytical error were added in the study to compare the performance of PBRTQC and RARTQC protocols: constant, random, and proportional errors. The false alarm rate and error detection charts were used to assess the protocols.

Results: The study showed that RARTQC outperformed PBRTQC. RARTQC, compared with the PBRTQC, improved the trimmed average number of patients affected before detection (tANPed) at total allowable error by about 50% for both constant and proportional errors.

Conclusions: The regression step in the RARTQC framework removes autocorrelation in the test results, allows researchers to add additional variables, and improves data transformation. RARTQC is a powerful framework for real-time quality control research.
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http://dx.doi.org/10.1093/clinchem/hvab115DOI Listing
August 2021

Crystal structure of the MyRF ICA domain with its upstream β-helical stalk reveals the molecular mechanisms underlying its trimerization and self-cleavage.

Int J Biol Sci 2021 13;17(11):2931-2943. Epub 2021 Jul 13.

State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fujian College, University of Chinese Academy of Sciences, 155 Yangqiao Road West, Fuzhou, 350002, China.

Myelin gene regulatory factor (MyRF), a novel membrane transcription factor expressed on the endoplasmic reticulum membrane, functions as a trimer. The trimerization of MyRF is associated with a fragment between the DNA binding domain and transmembrane domain that shares homology with the triple-β-helix and intramolecular chaperone autocleavage (ICA) domain of phage tailspike proteins. The molecular details of these domains in eukaryotes have not been elucidated. Here, we present the crystal structure of the MyRF ICA domain with its upstream β-helical stalk, determined at 2.4Å resolution. The structure showed that its upstream β-helical stalk is different from the triple β-helix reported before. This is the first structure of the mammalian protein with a triple β-helix. Structure analysis demonstrated that the triple α-helical coiled-coil formed at the MyRF ICA domain C-terminal was the main driving force for the trimerization. Additionally, our findings showed that MyRF was cleaved via a highly conserved serine-lysine catalytic dyad mechanism and that cleavage would be activated only if the ICA domains were organized as trimers. In contrast to the viral ICA domain, almost no interaction was found between the MyRF ICA domain and its upstream neighboring β-helix of the stalk; thus, activation of self-cleavage may not be triggered by the upstream region of the ICA domain, contrary to the observations made in phages. These findings provided an important insight into the molecular mechanisms of MyRF trimerization and self-cleavage.
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http://dx.doi.org/10.7150/ijbs.57673DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326128PMC
July 2021

The Effectiveness of Lapatinib in HER2-Positive Metastatic Breast Cancer Patients Pretreated With Multiline Anti-HER2 Treatment: A Retrospective Study in China.

Technol Cancer Res Treat 2021 Jan-Dec;20:15330338211037812

Department of Breast Cancer Center, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, People's Republic of China.

Background: The aim of this study was to evaluate the effectiveness of lapatinib in human epidermal growth factor receptor 2 (HER2)-positive metastatic breast cancer.

Methods: We retrospectively reviewed the medical records of patients who received lapatinib for salvage treatment at any line setting from January 1, 2007 to August 31, 2019 at Shandong Cancer Hospital and Institute.

Results: A total of 115 (89.1%) patients were included in the study. In the overall cohort, the median disease-free survival (DFS) was 19.0 months; the median progression-free survival (PFS), 6.3 months; and median overall survival (OS), 88.0 months, with 32.2% of patients alive at 5 years. In the second line setting, the median PFS among trastuzumab, lapatinib, and trastuzumab plus lapatinib were 4.2 months, 5.2 months, and 7.3 months, respectively ( = 0.004). No significant differences between the median PFSs and OSs of the different line salvage treatments with lapatinib was observed (all 0.05). For brain metastasis patients, the median PFSs in first line, second line, and more than 3 lines were 7.2 months, 4.5 months, and 6.3 months, respectively.

Conclusions: Our findings suggest that patients would benefit more from trastuzumab plus lapatinib than from lapatinib or trastuzumab alone for second line treatment in the advanced stages of the disease. Lapatinib could be used as an alternative selection for HER2-positive metastasic breast cancer patients when there is disease progression after trastuzumab or pyrotinib treatment, which is used as part of China's national health insurance.
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http://dx.doi.org/10.1177/15330338211037812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351035PMC
August 2021

Upregulated NLGN1 predicts poor survival in colorectal cancer.

BMC Cancer 2021 Aug 2;21(1):884. Epub 2021 Aug 2.

Department of Pathology, Union Hospital, Fujian Medical University, 29 Xin-Quan Road, Fuzhou, Fujian, 350001, People's Republic of China.

Background: Neuroligin1 (NLGN1) is a main component of excitatory glutamatergic synapses complex and is important for synapse assembly and function. The clinical value of NLGN1 in colorectal cancer (CRC) is not clear.

Methods: We obtained the expression data of 1143 CRC patients from 3 independent Gene Expression Omnibus (GEO) datasets (GSE32323, GSE24551, GSE39582) and The Cancer Genome Atlas (TCGA) to make the comparison of the NLGN1 expression level between CRC tissues and matched noncancerous tissues, and to evaluate its value in predicting survival of CRC patients. At the protein level, these results were further confirmed by immunohistochemical staining of 52 CRC samples in our own centre. Finally, the function of NLGN1 was explored by gene set enrichment analysis (GSEA).

Results: Increased mRNA and protein levels of NLGN1 expression were associated with worse overall survival or recurrence-free survival in CRC patients from 2 GEO datasets, the TCGA database, and our cohort. In addition, multivariate regression analysis showed that NLGN1 was an independent poor prognostic factor of survival in patients with CRC in TCGA database (OR = 2.524, P = 0.010). Functional analysis revealed that NLGN1 was correlated with function involving the Hedgehog signaling pathway, mismatch repair process, and some material metabolism processes.

Conclusions: This study is the first to implicate and verify NLGN1 as a new poor prognostic marker for CRC.
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http://dx.doi.org/10.1186/s12885-021-08621-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327451PMC
August 2021

Hypermethylation of Cyclin D2 Predicts Poor Prognosis of Hepatitis B Virus-Associated Hepatocellular Carcinoma after Hepatectomy.

Tohoku J Exp Med 2021 07;254(3):233-243

Department of Hepatology, Qilu Hospital, Cheeloo College of Medicine, Shandong University.

Prognosis of patients with hepatocellular carcinoma remains poor because of progression of hepatocellular carcinoma and high recurrence rates. Cyclin D2 (CCND2) plays a vital role in regulating the cell cycle; indeed, aberrant methylation of CCND2 is involved in the development of hepatocellular carcinoma. Therefore, we aimed to investigate levels of CCND2 methylation in patients with hepatitis B virus (HBV)-associated hepatocellular carcinoma and to evaluate its prognostic significance after hepatectomy. In total, 257 subjects were enrolled (166 hepatocellular carcinoma patients undergoing surgical resection, 61 chronic hepatitis B (CHB) patients, and 30 healthy controls). CCND2 methylation in peripheral blood mononuclear cells was measured quantitatively using MethyLight. We found that CCND2 methylation levels in patients with HBV-associated hepatocellular carcinoma were significantly higher than in CHB patients (P < 0.001) or healthy controls (P < 0.001). Within the hepatocellular carcinoma group, CCND2 methylation levels were higher in patients with portal vein invasion, early tumor recurrence, TNM III/IV stage, and tumor size ≥ 5 cm (P < 0.05). Furthermore, higher levels of CCND2 methylation were associated with worse overall survival and disease-free survival (P = 0.005 and P < 0.001, respectively). Multivariate analysis identified CCND2 methylation as an independent prognostic factor for early tumor recurrence (P = 0.021), overall survival (P = 0.022), and disease-free survival (P < 0.001) in hepatocellular carcinoma patients after resection. In conclusion, hypermethylation of CCND2 may have clinical utility for predicting a high risk of poor prognosis and early tumor recurrence in patients with HBV-associated hepatocellular carcinoma after hepatectomy.
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http://dx.doi.org/10.1620/tjem.254.233DOI Listing
July 2021

The feasibility and advantages of immediate removal of urinary catheter after lobectomy: A prospective randomized trial.

Nurs Open 2021 Jul 30. Epub 2021 Jul 30.

Department of Thoracic Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang, China.

Aim: This study aims to evaluate the feasibility and advantages of immediate urinary catheter removal compared with prolonged indwelling catheterization in lung cancer lobectomy.

Design: This study was designed as a prospective, single-centre, randomized and open-label clinical study.

Methods: People with lung cancer undergoing lobectomy/pneumonectomy were recruited and randomly allocated to two groups. One group had their urinary catheter removed immediately while the other group had it removed 48 hr after surgery.

Results: No significant difference in the incidence of postoperative urinary retention (POUR) was observed between the two groups. However, the incidence of postoperative catheter-associated urinary tract infection (CAUTI) in the immediate removal group (6.7%) was lower than the control group (17.2%) (p = .030). Furthermore, the incidence of catheter-associated emergence agitation (CAEA) in the control group (25.3%) was higher than the immediate removal group (8.9%) (p = .007). The average length of hospital stay of the immediate removal group [6.51(4-11) days] was shorter than the control group [7.20(5-12) days] (p = .002). Immediate removal of urinary catheter appeared to have fewer complications and shorter hospital stay than delayed removal.
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http://dx.doi.org/10.1002/nop2.1006DOI Listing
July 2021

Self-adaptive metasurface platform based on computer vision.

Opt Lett 2021 Aug;46(15):3520-3523

Programmable metasurfaces allow real-time electromagnetic (EM) manipulation in a digital manner, showing great potential to construct advanced multifunctional EM devices. However, the current programmable metasurfaces typically need human participation to achieve various EM functions. In this Letter, we propose, design, and construct a self-adaptive metasurface platform that can achieve beam control automatically based on image recognition. Such a platform is composed of a metasurface with 36×36 active units, a single-board computer, and two independent cameras that can detect the position of the objects. The single-board computer, Raspberry Pi, is used to calculate the information of the objects and generate the coding sequences to control the digital metasurface based on a low complexity binocular localization algorithm. Such a smart metasurface platform can generate different beams according to the location of the receiver and can be used as intelligent antennas in future communications and radars.
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http://dx.doi.org/10.1364/OL.427527DOI Listing
August 2021

Genetically determined selenium concentrations and risk for autoimmune diseases.

Nutrition 2021 Jun 24;91-92:111391. Epub 2021 Jun 24.

Department of Epidemiology, Zhejiang Chinese Medical University School of Public Health, Hangzhou, China. Electronic address:

Objective: Observational epidemiologic studies have reported a relationship between selenium status and risk for autoimmune diseases. However, the associations are susceptible to confounding or reverse causality. Thus, the aim of this study was to investigate the potential causal associations of selenium concentrations with the risk for common autoimmune diseases using a two-sample Mendelian randomization (MR) design.

Methods: A meta-analysis of genome-wide association studies (GWASs) of selenium among 9639 individuals of European ancestry was used to identify genetic instruments. Summary statistics of systemic lupus erythematosus, rheumatoid arthritis, and inflammatory bowel disease were obtained from publicly available GWASs, respectively. We conducted MR study using the inverse-variance weighted method, supplemented with weighted median and likelihood-based methods as sensitivity analysis. Cochran Q test and MR-Egger regression were used to detect heterogeneity and potential directional pleiotropy. MR-Pleiotropy RESidual Sum and Outlier test was used to identify outlier single-nucleotide polymorphisms.

Results: Genetically predicted high selenium level was associated with a decreased risk for SLE (odds ratio, 0.85; 95% confidence interval, 0.77-0.93; P = 0.001) per natural log-transformed selenium concentrations, with similar results in sensitivity analyses. No evidence of heterogeneity, pleiotropy, or outlier single-nucleotide polymorphisms were detected (all P > 0.05). However, genetically determined selenium concentrations may be not associated with risk for rheumatoid arthritis or inflammatory bowel disease in the primary analysis and subsequent sensitivity analyses.

Conclusions: The present study suggested a protective role of selenium on the risk for systemic lupus erythematosus. Further studies are warranted to elucidate the underlying mechanisms.
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http://dx.doi.org/10.1016/j.nut.2021.111391DOI Listing
June 2021
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