Publications by authors named "Ying Zhao"

1,923 Publications

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Critical view of safety in laparoscopic cholecystectomy: A prospective investigation from both cognitive and executive aspects.

Front Surg 2022 1;9:946917. Epub 2022 Aug 1.

Department of Pancreatic Surgery, West China Hospital, Sichuan University, Chengdu, China.

Background: The achievement rate of the critical view of safety during laparoscopic cholecystectomy is much lower than expected. This original study aims to investigate and analyze factors associated with a low critical view of safety achievement.

Materials And Methods: We prospectively collected laparoscopic cholecystectomy videos performed from September 2, 2021, to September 19, 2021, in Sichuan Province, China. The artificial intelligence system, SurgSmart, analyzed videos under the necessary corrections undergone by expert surgeons. Also, we distributed questionnaires to surgeons and analyzed them along with surgical videos simultaneously.

Results: We collected 169 laparoscopic cholecystectomy surgical videos undergone by 124 surgeons, among which 105 participants gave valid answers to the questionnaire. Excluding those who conducted the bail-out process directly, the overall critical view of safety achievement rates for non-inflammatory and inflammatory groups were 18.18% (18/99) and 9.84% (6/61), respectively. Although 80.95% (85/105) of the surgeons understood the basic concept of the critical view of safety, only 4.76% (5/105) of the respondents commanded all three criteria in an error-free way. Multivariate logistic regression results showed that an unconventional surgical workflow (OR:12.372,  < 0.001), a misunderstanding of the 2nd (OR: 8.917,  < 0.05) and 3rd (OR:8.206,  < 0.05) criterion of the critical view of safety, and the don't mistake "fundus-first technique" as one criterion of the critical view of safety (OR:0.123,  < 0.01) were associated with lower and higher achievements of the critical view of safety, respectively.

Conclusions: The execution and cognition of the critical view of safety are deficient, especially the latter one. Thus, increasing the critical view of safety surgical awareness may effectively improve its achievement rate.
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http://dx.doi.org/10.3389/fsurg.2022.946917DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377448PMC
August 2022

Characterization of DON sources linked with water quality for inland water bodies in the Songnen Plain of Northeast China.

Authors:
Ying Zhao

Environ Sci Pollut Res Int 2022 Aug 17. Epub 2022 Aug 17.

Key Research Institute of Yellow River Civilization and Sustainable Development, Henan University, Kaifeng, 475001, China.

Dissolved organic nitrogen (DON) in inland water bodies plays an important role in the global nitrogen and carbon cycles. A total of 14 fresh water and 5 brackish water bodies (electrical conductivity (EC) threshold value = 1200 μS cm) were selected to assess the dynamics of DON for inland water bodies in the semiarid Songnen Plain of Northeast China. The spatial characteristics of DON concentrations, chromophoric dissolved organic matter (CDOM) absorption, CDOM fluorescent components (two humic-like C1 and C3, one tryptophan-like C2) and their correlations with water quality for these 19 water bodies were firstly evaluated using excitation-emission matrix (EEM) fluorescence spectroscopy coupled with parallel factor (PARAFAC) with the data collected in May 2021. Results showed that there were significant differences in DON concentrations, CDOM absorption a(254) and CDOM fluorescent components C1-C3 between fresh water and brackish water bodies (t test, p < 0.01), respectively. DON concentrations were moderately correlated with EC (R = 0.766, p < 0.01), trophic state index (TSI) (R = 0.757, p < 0.01) and chemical oxygen demand (COD) (R = 0.740, p < 0.01) for all 19 water bodies, respectively, suggesting that DON can be used as the potential predictors for TSI and COD. Further, there was a significant positive linear relationship between the CDOM fluorescent component C1 and TN (R = 0.654, p < 0.01), DON (R = 0.746, p < 0.01) or COD (R = 0.893, p < 0.01), respectively, indicating that the nitrogen and carbon in CDOM were originated from similar terrestrial humic-like substances. These results demonstrated that CDOM humic-like fluorescent component C1 can be used for the best optical predictors for nutrients providing a possible way to directly monitor DON and associated with water quality for inland water bodies in the semiarid regions of Northeast China, which has potential implication for inland water bodies with similar characteristics.
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http://dx.doi.org/10.1007/s11356-022-22452-8DOI Listing
August 2022

A report of Pectobacterium carotovorum subsp. actinidiae causing kiwifruit bacterial canker in GuangXi, China.

Plant Dis 2022 Aug 15. Epub 2022 Aug 15.

Nanjing Agricultural University, Department of Plant Pathology, No.1, Weigang, Nanjing, China, 210095;

A bacterial pathogen strain was isolated from susceptible tissue of Hongyang variety kiwifruit in Zhongfeng Town, Ziyuan County, Guilin City, Guangxi, China. Due to the relatively single variety of kiwifruit in Guangxi, the control technology of fruit farmers is backward, and the climate is humid, which is suitable for the growth of pathogenic bacteria, resulting in frequent occurrence of diseases. In this study, the pathogen strain was identified on the basis of morphological, physiological and biochemical tests, 16S rRNA , PCR detection with specific primers, and Biolog analysis. The results showed that a tobacco allergic reaction could be induced by inoculation with the pathogenic bacteria, Additionally, brown necrotic plaques appeared on kiwifruit leaves, necrotic phloem lesions appeared, and wounds on kiwifruit branches turned brown. The characteristics identified by morphological, physiological, biochemical and Biolog identification were similar to those caused by Pectobacterium sp. Through 16S rRNA sequence analysis and PCR identification with specific primers, bands with a size corresponding to target bands, indicating that the pathogen was Pectobacterium carotovorum subsp. actinidiae . This is the first report of kiwifruit canker disease caused by Pectobacterium carotovorum subsp. actinidiae in Guangxi, China. In addition, through this study, a preliminary understanding of the pathogen has been obtained, which will lay the foundation for the prevention and control of the disease in the future.
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http://dx.doi.org/10.1094/PDIS-01-22-0157-REDOI Listing
August 2022

Cardiac manifestations in a Chinese cohort of fetuses from mothers with anti-Ro and anti-La antibodies.

Front Pediatr 2022 28;10:904138. Epub 2022 Jul 28.

Echocardiography Medical Center, Maternal-Fetal Medicine Center in Fetal Heart Disease, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.

Objectives: To analyze the clinical characteristics, echocardiographic features, and prognosis of fetuses based on three groups of cardiac manifestations associated with maternal anti-Ro and anti-La antibodies in China. This study included three groups: the isolated-arrhythmia, isolated-endocardial fibroelastosis (EFE), and mixed groups.

Methods: We prospectively evaluated 36 fetuses with cardiac manifestations due to maternal anti-Ro and anti-La antibodies from our center between 2016 and 2020 in China. Clinical and echocardiographic data were collected.

Results: There were 13 patients (36%) in the isolated-arrhythmia group, eight (22%) in the isolated-EFE group, and 15 (42%) in the mixed group. All patients in the isolated-EFE group presented with mild EFE. Severe EFE was identified in four patients (27%) in the mixed group. Atrioventricular block (AVB) was more common in the isolated-arrhythmia group (13, 100%) than in the mixed group (6, 40%; = 0.001). Moderate-severe mitral regurgitation ( = 0.006), dilated cardiomyopathy (DCM, = 0.017), and low cardiovascular profile scores ( = 0.013) were more common in the mixed group than in the other two groups. Twenty-one mothers decided to terminate the pregnancy and 15 fetuses were born with regular perinatal treatment. They all survived at 1 year of age. One patient in the isolated-arrhythmia group and two in the mixed group required a pacemaker due to third-degree AVB or atrioventricular junctional rhythm. Five patients in the isolated-EFE group and five in the mixed group had no DCM or heart failure and the location of mild EFE was significantly reduced.

Conclusion: Fetal cardiac manifestations due to maternal anti-Ro and anti-La antibodies can be divided into three groups, i.e., the isolated-arrhythmia, isolated-EFE, and mixed groups. AVB usually occurs in the isolated-arrhythmia group. Severe EFE, moderate-severe mitral regurgitation, and DCM mainly appear in the mixed group. Location of mild EFE significantly reduces after birth and the outcome of fetuses with mild EFE depends on the presence of arrhythmia and its subtypes.
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http://dx.doi.org/10.3389/fped.2022.904138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371606PMC
July 2022

A vegan diet improves insulin resistance in individuals with obesity: a systematic review and meta-analysis.

Diabetol Metab Syndr 2022 Aug 13;14(1):114. Epub 2022 Aug 13.

Department of Endocrinology, The Second Hospital of Jilin University, No. 218, Ziqiang Street, Nanguan District, Changchun, China.

Background: A vegan diet has benefits on weight reduction and on the parameters of glucose and lipid metabolism. This meta-analysis aimed to investigate the efficacy of plant-based diets on insulin resistance and blood lipids in patients with obesity.

Methods: PubMed, Embase, and the Cochrane Library were searched for available papers published up to March 2021. The primary outcome was insulin resistance which was assessed by Homeostasis Model Assessment Insulin Resistance (HOMA-IR), other metabolic parameters measures including the pre/post-diet changes in triglycerides, HDL-cholesterol, total cholesterol, LDL-cholesterol. All analyses were performed using the random-effects model.

Results: Six studies (seven datasets) were included. Compared with baseline, the plant-based diet improved the HOMA-IR (SMD = 1.64, 95%CI 0.95, 2.33; I = 91.8%, P < 0.001), total cholesterol (SMD = 2.51, 95% CI 0.88, 4.13; I = 98.0%, P < 0.001), HDL-cholesterol (SMD = 1.55, 95% CI 0.66, 2.44; I = 92.0%, P < 0.001), and LDL-cholesterol (SMD = 2.50, 95% CI 1.30, 3.70; I = 94.4%, P < 0.001), but not the triglycerides (SMD = - 0.62, 95% CI - 1.92, 0.68; I = 97.8%, P < 0.001). The sensitivity analyses showed that the results were robust.

Conclusions: In obese individuals with insulin resistance, a vegan diet improves insulin resistance and dyslipidemia, except for triglycerides.
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http://dx.doi.org/10.1186/s13098-022-00879-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9375406PMC
August 2022

Deciphering the molecular mechanism underlying the effects of epimedium on osteoporosis through system bioinformatic approach.

Medicine (Baltimore) 2022 Aug;101(32):e29844

Integrated Traditional Chinese and Western Medicine Hospital of Shenzhen, Bao'an District, Shenzhen, Guangdong Province, China.

Epimedium has gained widespread clinical application in Traditional Chinese Medicine, with the functions of promoting bone reproduction, regulating cell cycle and inhibiting osteoclastic activity. However, its precise cellular pharmacological therapeutic mechanism on osteoporosis (OP) remains elusive. This study aims to elucidate the molecular mechanism of epimedium in the treatment of OP based on system bioinformatic approach. Predicted targets of epimedium were collected from TCMSP, BATMAN-TCM and ETCM databases. Differentially expressed mRNAs of OP patients were obtained from Gene Expression Omnibus database by performing Limma package of R software. Epimedium-OP common targets were obtained by Venn diagram package for further analysis. The protein-protein interaction network was constructed using Cytoscape software. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were carried out by using clusterProfiler package. Molecular docking analysis was conducted by AutoDock 4.2 software to validate the binding affinity between epimedium and top 3 proteins based on the result of protein-protein interaction. A total of 241 unique identified epimedium targets were screened from databases, of which 62 overlapped with the targets of OP and were considered potential therapeutic targets. The results of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that these targets were positive regulation of cell cycle, cellular response to oxidative stress and positive regulation of cell cycle process as well as cellular senescence, FoxO, PI3K-Akt, and NF-kappa B signaling pathways. Molecular docking showed that epimedium have a good binding activity with key targets. Our study demonstrated the multitarget and multi-pathway characteristics of epimedium on OP, which elucidates the potential mechanisms of epimedium against OP and provides theoretical basis for further drug development.
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http://dx.doi.org/10.1097/MD.0000000000029844DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371495PMC
August 2022

Molecular insights into the catalytic oxidation of methanol-to-olefins wastewater with phosphoric acid modified sludge biochar.

Chemosphere 2022 Aug 6;307(Pt 2):135938. Epub 2022 Aug 6.

Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China. Electronic address:

With the development of methanol-to-olefin (MTO) process, the effective disposal of wastewater was one key factor for the long-period and benign development of this technology. Herein, a sludge-based biochar catalyst (GSC-P) was synthesized and used in photo-Fenton reaction for the degradation of MTO wastewater from the outlet of a biological aerated filter. More iron was distributed on the surface of GSC-P catalyst, facilitating the photo-Fenton oxidation of MTO wastewater, with chemical oxygen demand (COD) removal rate of 75.4% and total organic carbon (TOC) removal rate of 62.5%. The 2223 unique molecular formulas assigned by a Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) in the original MTO wastewater showed that CHO compounds shared the lowest peak numbers (20.2%) but the highest peak abundance (51.6%) among the four groups. Besides, lipids, unsaturated hydrocarbons, lignins and proteins were the main structural types. After photo-Fenton treatment of 60 min, there were 56.7%-74.0% of compounds removed by the analysis of van Krevelen diagram, indicating that the MTO wastewater was degraded efficiently. Three possible evolution processes of dissolved organic matters during the photo-Fenton reaction were disclosed at the molecular-level.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135938DOI Listing
August 2022

Mechanism of Enhanced Oral Absorption of a Nano-Drug Delivery System Loaded with Trimethyl Chitosan Derivatives.

Int J Nanomedicine 2022 29;17:3313-3324. Epub 2022 Jul 29.

College of Pharmacy, Guilin Medical University, Guilin, 541199, People's Republic of China.

Introduction: In the previous study, nanoparticles coated with trimethyl chitosan (TMC) derivatives (PPTT-NPs) could promote the oral bioavailability of panax notoginseng saponins (PNS). Herein, we chose PPTT-NPs as a model drug to study the property and mechanism of intestinal absorption in vitro and in vivo.

Methods: The stability of PPTT-NPs was evaluated using simulated gastric fluid and simulated intestinal fluid. The uptake and transport of PPTT-NPs were investigated in Caco-2 and Caco-2&HT29 co-culture cells. The biosafety, intestinal permeability, adhesion, and absorption mechanism of PPTT-NPs were investigated using SD rats in vivo. The live imaging and biodistribution of PPTT-NPs were observed by IVIS. Furthermore, the effects on CYP3A4 of PPTT-NPs were investigated using testosterone as the probe substrate.

Results: The results of the stability assay showed that PPTT-NPs had a strong tolerance to the pH and digestive enzymes in the gastrointestinal environment. In vitro cell experiments showed that the uptake of drugs exhibited a time-dependent. When the ratio of TMC-VB and TMC-Cys was 1:3, the uptake capacity of PPTT-NPs was the highest. PPTT-NPs could enhance the paracellular transport of drugs by reversibly opening a tight junction. Animal experiments demonstrated that PPTT-NPs have good biological safety. PPTT-NPs had good adhesion and permeability to small intestinal mucosa. Meanwhile, PPTT-NPs needed energy and various protein to participate in the uptake of drugs. The live imaging of NPs illustrated that PPTT-NPs could prolong the residence time in the intestine. Moreover, TMC-VB and TMC-Cys could reduce the metabolism of drugs by inhibiting CYP3A4 to a certain extent.

Conclusion: The results show that TMC-VB and TMC-Cys are effective in the transport of PPTT-NPs. PPTT-NPs can increase the intestinal adhesion of drugs and exert high permeation by intestinal enterocytes which demonstrate significant and efficient potential for oral delivery of the BCS III drugs.
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http://dx.doi.org/10.2147/IJN.S358832DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9346306PMC
August 2022

Interferon-alpha responsible EPN3 regulates hepatitis B virus replication.

Front Med (Lausanne) 2022 22;9:944489. Epub 2022 Jul 22.

Department of Pathogen Biology, Shenyang Medical College, Shenyang, China.

Hepatitis B virus (HBV) infection remains a major health problem worldwide, and the current antiviral therapy, including nucleoside analogs, cannot achieve life-long cure, and clarification of antiviral host immunity is necessary for eradication. Here, we found that a clathrin-binding membrane protein epsin3 (EPN3) negatively regulates the expression of HBV RNA. EPN3 expression was induced by transfection of an HBV replicon plasmid, and reduced HBV-RNA level in hepatic cell lines and murine livers hydrodynamically injected with the HBV replicon plasmid. Viral RNA reduction by EPN3 was dependent on transcription, and independent from epsilon structure of viral RNA. Viral RNA reduction by overexpression of p53 or IFN-α treatment, was attenuated by knockdown of EPN3, suggesting its role downstream of IFN-α and p53. Taken together, this study demonstrates the anti-HBV role of EPN3. The mechanism how it decreases HBV transcription is discussed.
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http://dx.doi.org/10.3389/fmed.2022.944489DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354525PMC
July 2022

A fluorescence biosensor based on double-stranded DNA and a cationic conjugated polymer coupled with exonuclease III for acrylamide detection.

Int J Biol Macromol 2022 Aug 4;219:346-352. Epub 2022 Aug 4.

School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan 453003, PR China; Xinxiang Key Laboratory of Clinical Psychopharmacology, Xinxiang Medical University, Xinxiang 453003, PR China.

As a toxic substance on human health produced in food thermal treatment, simple analytical approaches are highly desired for the detection of acrylamide (ACR) in foods. With the aid of exonuclease III (Exo III), a simple fluorescence sensor was proposed based on carboxyfluorescein-labeled double-stranded DNA (FAM-dsDNA) and a cationic conjugated polymer (PFP). Fluorescence resonance energy transfer (FRET) efficiency between FAM and PFP was changed with and without ACR. When ACR was present, ACR and single-stranded DNA (P1, ssDNA) formed an adduct, allowing free FAM-labeled complementarity strand DNA (P2, FAM-csDNA) to appear in the solution and avoiding the digestion of P2 by Exo III. After the addition of PFP, the interaction of PFP and FAM induced strong FRET. Under optimized conditions, ACR was detected with a limit of detection (LOD) of 0.16 μM. According to this biosensor, a LOD of 1.3 μM in water extract samples was observed with a good recovery rate (95-110 %).
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.251DOI Listing
August 2022

Oxidative stress-mediated mitochondrial fission promotes hepatic stellate cell activation via stimulating oxidative phosphorylation.

Cell Death Dis 2022 Aug 6;13(8):689. Epub 2022 Aug 6.

Regeneration Medicine Research Center, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, P. R. China.

Previous studies have demonstrated dysregulated mitochondrial dynamics in fibrotic livers and hepatocytes. Little is currently known about how mitochondrial dynamics are involved, nor is it clear how mitochondrial dynamics participate in hepatic stellate cell (HSC) activation. In the present study, we investigated the role of mitochondrial dynamics in HSC activation and the underlying mechanisms. We verified that mitochondrial fission was enhanced in human and mouse fibrotic livers and active HSCs. Moreover, increased mitochondrial fission driven by fis1 overexpression could promote HSC activation. Inhibiting mitochondrial fission using mitochondrial fission inhibitor-1 (Mdivi-1) could inhibit activation and induce apoptosis of active HSCs, indicating that increased mitochondrial fission is essential for HSC activation. Mdivi-1 treatment also induced apoptosis in active HSCs in vivo and thus ameliorated CCl-induced liver fibrosis. We also found that oxidative phosphorylation (OxPhos) was increased in active HSCs, and OxPhos inhibitors inhibited activation and induced apoptosis in active HSCs. Moreover, increasing mitochondrial fission upregulated OxPhos, while inhibiting mitochondrial fission downregulated OxPhos, suggesting that mitochondrial fission stimulates OxPhos during HSC activation. Next, we found that inhibition of oxidative stress using mitoquinone mesylate (mitoQ) and Tempol inhibited mitochondrial fission and OxPhos and induced apoptosis in active HSCs, suggesting that oxidative stress contributes to excessive mitochondrial fission during HSC activation. In conclusion, our study revealed that oxidative stress contributes to enhanced mitochondrial fission, which triggers OxPhos during HSC activation. Importantly, inhibiting mitochondrial fission has huge prospects for alleviating liver fibrosis by eliminating active HSCs.
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http://dx.doi.org/10.1038/s41419-022-05088-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357036PMC
August 2022

VvTOR interacts with VvSnRK1.1 and regulates sugar metabolism in grape.

Planta 2022 Aug 6;256(3):56. Epub 2022 Aug 6.

College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, People's Republic of China.

Main Conclusion: VvTOR interacts with VvSnRK1.1 and regulates sugar accumulation and sugar-related genes expression in grape. Target of rapamycin (TOR) and sucrose-non-fermenting-related protein kinase 1.1 (SnRK1.1) both are critical proteins in plant sugar metabolism. Glucose-TOR signaling dictates transcriptional reprogramming of gene sets involved in central and secondary metabolism, cell cycle, transcription, signaling, transport and folding. SnRK1.1 is involved in sucrose-induced hypocotyl elongation. However, the relationship of TOR and SnRK1.1 in regulating sugar metabolism is unclear. In the study, we utilized grape (Vitis vinifera) calli to explore the relationship between TOR and SnRK1.1 in the sugar metabolism. We found that VvTOR interacted with VvSnRK1.1. By subcellular localization, VvTOR was found in the nucleus and cell membrane. Transgenic grape calli achieved by Agrobacterium-mediated transformation contained less glucose compared to WT calli. The fructose contents were markedly increased in the overexpressing VvTOR (OE-VvTOR), OE-VvTOR + RNAi-VvSnRK1.1 and RNAi-VvTOR + OE-VvSnRK1.1 transgenic calli. Sucrose contents were significantly increased in the OE-VvTOR transgenic calli and reduced in the OE-VvTOR + RNAi-VvSnRK1.1 transgenic calli, which implied that the pathway of VvTOR improving sucrose content might need the expression of VvSnRK1.1. VvTOR interacted with VvSnRK1.1 and regulated sugar metabolism in grape. These results suggest that there is a crosstalk between TOR and SnRK1.1 in plant sugar metabolism.
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http://dx.doi.org/10.1007/s00425-022-03969-1DOI Listing
August 2022

Precise portrayal of microscopic processes of wastewater biofilm formation: Taking SiO as the model carrier.

Sci Total Environ 2022 Aug 2;849:157776. Epub 2022 Aug 2.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, Jiangsu, PR China. Electronic address:

Precise characterization of the microscopic processes of wastewater biofilm formation is essential for regulating biofilm behavior. Nevertheless, it remains a great challenge. This study investigated biofilm formation on SiO carriers under gradually increasing shear force combining the extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory in a Couette-Taylor reactor, and precisely revealed the micro-interface interaction and species colonization during biofilm formation. The results indicated that bacterial reversible adhesion distance on SiO carrier surface was 3.06 ± 0.48 nm. Meanwhile, the secondary minimum of total XDLVO interaction energy could be used as a novel indicator to distinguish biofilm formation stages. The revealed biofilm formation stages were also confirmed by the electrochemical analysis. Additionally, the pioneer species that colonized at first were Comamonadaceae, Azospira, Flavobacterium and Azonexus, while keystone species such as Hydrogenophaga, AKYH767, Aquimonas and Ignavibacterium determined the stability of microbial community. In conclusion, this study provided a methodological example to study wastewater biofilm micro-interface behavior through the integration of an experimental platform as well as multiple monitoring and analysis methods, which opened up new perspectives for biofilm research and provided useful guidance for the regulation of biofilm-related treatment processes and new technology development.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157776DOI Listing
August 2022

Effect of Straw and Wood Ash on Soil Carbon Sequestration and Bacterial Community in a Calcareous Soil.

Front Microbiol 2022 18;13:926506. Epub 2022 Jul 18.

Key Laboratory of Coastal Eco Hydrological Process and Environmental Safety, School of Resources and Environmental Engineering, Ludong University, Yantai, China.

Soil fertility can be improved by effectively utilizing agricultural waste. Straw can supply energy and wood ash adds nutrients to improve soil quality. However, few kinds of research have investigated the effect of wood ash and straw on soil carbon sequestration and the soil bacterial population, particularly in calcareous soils. The main goal of this current study was to quantify the impact of a combination of wood ash and straw on the indicators described above using stable δC isotope analyses by applying wheat straw to calcareous soil under a long-term C crop rotation. The incubation experiment included four treatments as follows: (i) no amendment (Control); (ii) amendment with wood ash (W); (iii) amendment with straw (S); and (iv) a combined amendment of straw and wood ash (SW). Our results showed that sequestration of soil inorganic carbon (SIC) in the SW and W treatments was higher (an average of 7.78%) than that in the S and Control treatments. The sequestered soil organic carbon (SOC) in the SW treatment was 1.25-fold greater than that in the S treatment, while there was no evident effect on the SOC content compared with straw alone. The microbial biomass carbon increased under SW by 143.33%, S by 102.23%, and W by 13.89% relative to control. The dissolved organic carbon increased under SW by 112.0%, S by 66.61%, and W by 37.33% relative to the control. The pH and electrical conductivity were higher in the SW and W treatments than in the S treatment and the control. The SW was conducive to maintaining soil enzymatic activities and bacterial diversity. Bacteroidetes and Actinobacteriota were dominant in SW, while the Acidobacteria phyla were dominant in the S treatment. The diversity of bacteria in the soil and community composition of the bacteria were predominantly assessed by the levels of water-soluble K, pH, and electrical conductivity. The incorporation of straw and wood ash is probably more effective at improving SIC and SOC sequestration and ameliorates the soil microhabitat.
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http://dx.doi.org/10.3389/fmicb.2022.926506DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339994PMC
July 2022

VCAM-1-binding peptide targeted cationic liposomes containing NLRP3 siRNA to modulate LDL transcytosis as a novel therapy for experimental atherosclerosis.

Metabolism 2022 Jul 30;135:155274. Epub 2022 Jul 30.

Department of Endocrinology, Institute of Geriatric Medicine, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430077, China; Department of Pharmacology, the Key Laboratory of Drug Target Researches and Pharmacodynamics Evaluation of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China. Electronic address:

Background: Activation of NLRP3 inflammasome accelerates the formation of atherosclerotic plaques. Here, we evaluated the effects of inflammation on the expression of the NLRP3 inflammasome in endothelial cells (ECs).

Methods: The effect of TNF-α on transcytosis of LDL was measured. VCAM-1 binding peptide targeting cationic liposomes (PCLs) were prepared as siRNA vectors. Methylated NLRP3 siRNA was encapsulated into the PCLs to knock down NLRP3 in vitro and in vivo. In rats with partial carotid ligation, TNF-α-induced LDL retention in the carotid artery endothelium was observed. In ApoE mice, NLRP3 siRNA-PCLs were injected intravenously to observe their effect on the formation of atherosclerosis.

Results: Our results showed that TNF-α upregulated NLRP3 in ECs, promoting the assembly of the NLRP3 inflammasome and processing of pro-IL-1β into IL-1β. Moreover, TNF-α accelerated LDL transcytosis in ECs. Knockdown of NLRP3 prevented TNF-α-induced NLPR3 inflammasome/IL-1β signaling and LDL transcytosis. Using optimized cationic liposomes to encapsulate methylated NLRP3 siRNA, resulting in targeting of VCAM-1-expressing ECs, to knockdown NLRP3, TNF-α-induced NLRP3 inflammasome activation and LDL transcytosis were prevented. Using the partial carotid ligation as an atherosclerosis rat model, we found that local administration of NLRP3 siRNA-PCLs efficiently knocked down NLPR3 expression in the carotid endothelium and dramatically attenuated the deposition of atherogenic LDL in carotid ECs in TNF-α-challenged rats. Furthermore, NLRP3 siRNA-PCLs were injected intravenously in ApoE mice, resulting in reduced plaque formation.

Conclusion: These findings established a novel strategy for targeting the NLRP3 inflammasome using NLRP3 siRNA-PCLs to interrupt LDL transcytosis, representing a potential novel therapy for atherosclerosis.
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http://dx.doi.org/10.1016/j.metabol.2022.155274DOI Listing
July 2022

Preliminary Application of Magnetization Transfer Imaging in the Study of Normal Uterus and Uterine Lesions.

Front Oncol 2022 14;12:853815. Epub 2022 Jul 14.

Department of MRI, The First People's Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, China.

Purpose: The aim of this study is to evaluate the utility of magnetization transfer (MT) imaging in the study of normal uterus and common uterine lesions.

Methods: This prospective study enrolled 160 consecutive patients with suspected uterine lesions. MT ratio (MTR) map was obtained by pelvic MT imaging on a 3.0T MRI scanner. Patients confirmed by pathology were divided into microscopic lesion group and lesion group, according to whether the maximum diameter of the lesion was less than 5 mm. After evaluating and eliminating patients with poor image quality by a three-point Likert scale, MTR values of lesions and normal endometrium, myometrium, and cervix were independently measured on the MTR map by two radiologists. Inter-reader agreement was evaluated. MTR values were compared among different uterine lesions and normal uterine structures using the Mann-Whitney U test with Bonferroni correction. Receiver operating characteristic curve was performed. The correlations between age and MTR values were explored by Pearson correlation analyses.

Results: A total of 96 patients with 121 uterine lesions in the lesion group and 41 patients in the microscopic lesion group were measured. The MTR values among normal endometrium, myometrium, and cervix were statistical significant differences ( < 0.05). There were significant differences between endometrial cancer and normal endometrium and between cervical cancer and normal cervix (both ≤ 0.001). Area under the curve (AUC) for diagnosing endometrial and cervical cancer were 0.73 and 0.86. Myometrial lesions had significantly higher MTR values than endometrial lesions and cervical cancer (both < 0.001), and the AUC for differentiating myometrial lesions from them were 0.89 and 0.94. MTR values of endometrial cancer were significantly higher than those of cervical cancer ( = 0.02). There was a critical correlation between age and MTR values in endometrial cancer ( = 0.81, = 0.04).

Conclusions: MTR values showed significant differences among normal uterine structures. It was valuable for diagnosing and differentiating uterine cancer. MTR values could differentiate myometrial lesions from endometrial or cervical lesions.
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http://dx.doi.org/10.3389/fonc.2022.853815DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331739PMC
July 2022

Lumbar venous fenestration malformation causing renal artery stenosis.

Rev Esp Cardiol (Engl Ed) 2022 Jul 27. Epub 2022 Jul 27.

Department of Radiology, the Second Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, China. Electronic address:

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http://dx.doi.org/10.1016/j.rec.2022.07.006DOI Listing
July 2022

Two large novel alpha-globin gene cluster deletions causing alpha(0)-thalassemia in two Chinese families.

Gene 2022 Oct 26;840:146767. Epub 2022 Jul 26.

Prenatal Diagnostic Center, Affiliated Dongguan Maternal and Child Health Care Hospital, Southern Medical University, Dongguan, China. Electronic address:

Introduction: Monosomy of terminal 16p13.3 is a relatively common subtelomeric abnormality, most affected individuals presented α-thalassemia, some also have mental retardation, developmental abnormalities and/or speech delay and facial dysmorphism, which is termed ATR-16 syndrome. Here, we reported two novel 16p13.3 deletions involving the α-globin gene cluster and multispecies conserved sequences (MCSs), causing only a phenotype of α-thalassemia.

Methods: Samples were collected from members of the two families and were subjected to haematological and comprehensive genetic analysis.

Results: The novel 108 Kb deletion in family A extends from the non-protein coding RNA gene (WASIR2) to the NPRL3 gene, removing MCS-R1 to R3. This deletion should arise de novo because it wasn't detected in both parents. The novel 336 Kb deletion in family B should extend from telomere to ∼ chr16:336000, removing the entire α-globin gene cluster. Carriers of these two deletions presented with microcytosis and hypochromic red cells, in accordance with a phenotype of α-thalassemia carrier.

Conclusion: Our study increases the mutation spectrum of α-thalassemia. MCSs deletion should be considered in clinical practice of thalassemia screening and diagnosis.
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http://dx.doi.org/10.1016/j.gene.2022.146767DOI Listing
October 2022

Association between apolipoprotein B/A1 and the risk of metabolic dysfunction associated fatty liver disease according to different lipid profiles in a Chinese population: A cross-sectional study.

Authors:
Ying Zhao

Clin Chim Acta 2022 Jul 26;534:138-145. Epub 2022 Jul 26.

Department of Clinical Laboratory, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, China. Electronic address:

Background And Aim: Metabolic dysfunction associated fatty liver disease (MAFLD) is the most common liver disease and dyslipidemia is commonly considered a prominent risk factor for MAFLD. This study was to investigate the association between the apolipoprotein B/A1 (apo B/A1) ratio and the risk of MAFLD based on new diagnostic criteria.

Methods: We conducted a cross-sectional study on 3341 participants. Restricted cubic spline (RCS) analyses, logistic regression, Synergistic effects analyses and stratified analyses were used to evaluate the association between the apo B/A1 ratio and the risk of MAFLD.

Results: The apo B/A1 ratio was nonlinearly related to the increased risk of MAFLD and the OR and 95% CI for the apo B/A1 95th percentile was 1.700 (1.004-2.879) compared with the 50th percentile. Each 1 SD increase in apo B/A1 ratio would increase the 1.313-fold risk of the risk of MAFLD in all participants and 1.46-fold risk in normolipidemic participants. Synergistic effects indicated elevated Apo B/A1 ratio and dyslipidemia collectively contributed to an increased risk of MAFLD [OR (95 %CI): 2.496(1.869-3.334)].

Conclusions: The apo B/A 1 ratio was a risk factor of the presence of MAFLD. Dyslipidemia and elevated the Apo B/A1 ratio can synergistically contributed to the risk of MAFLD.
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http://dx.doi.org/10.1016/j.cca.2022.07.014DOI Listing
July 2022

Label-free purification and characterization of optogenetically engineered cells using optically-induced dielectrophoresis.

Lab Chip 2022 Jul 29. Epub 2022 Jul 29.

State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China.

Optogenetically engineered cell population obtained by heterogeneous gene expression plays a vital role in life science, medicine, and biohybrid robotics, and purification and characterization are essential to enhance its application performance. However, the existing cell purification methods suffer from complex sample preparation or inevitable damage and pollution. The efficient and nondestructive label-free purification and characterization of the optogenetically engineered cells, HEK293-ChR2 cells, is provided here using an optically-induced dielectrophoresis (ODEP)-based approach. The distinctive crossover frequencies of the engineered cells and the unmodified cells enable effective separation due to the opposite DEP forces on them. The ODEP-based approach can greatly improve the purity of the separated cell population and especially, the ratio of the engineered cells in the separated cell population can be enhanced by 275% at a low transfection rate. The size and the membrane capacitance of the separated cell population decreases and increases, respectively, as the ratio of the engineered cells grows in the cell population, indicating that successful expression of ChR2 in a single HEK293 cell makes its size and membrane capacitance smaller and larger, respectively. The results of biohybrid imaging with the optogenetically engineered cells demonstrated that cell purification can improve the imaging quality. This work proves that the separation and purification of engineered cells are of great significance for their application in practice.
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http://dx.doi.org/10.1039/d2lc00512cDOI Listing
July 2022

Feedforward Control of Piezoelectric Ceramic Actuators Based on PEA-RNN.

Sensors (Basel) 2022 Jul 19;22(14). Epub 2022 Jul 19.

Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.

Multilayer perceptron (MLP) has been demonstrated to implement feedforward control of the piezoelectric actuator (PEA). To further improve the control accuracy of the neural network, reduce the training time, and explore the possibility of online model updating, a novel recurrent neural network named PEA-RNN is established in this paper. PEA-RNN is a three-input, one-output neural network, including one gated recurrent unit (GRU) layer, seven linear layers, and one residual connection in the linear layers. The experimental results show that the displacement linearity error of piezoelectric ceramics reaches 8.96 μm in the open-loop condition. After using PEA-RNN compensation, the maximum displacement error of piezoelectric ceramics is reduced to 0.465 μm at the operating frequency of 10 Hz, which proves that PEA-RNN can accurately compensate piezoelectric ceramics' dynamic hysteresis nonlinearity. At the same time, the training epochs of PEA-RNN are only 5% of the MLP, and fewer training epochs provide the possibility to realize online updates of the model in the future.
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http://dx.doi.org/10.3390/s22145387DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315871PMC
July 2022

Effects of High Ambient Temperature on Small Intestinal Morphology and Colonic Microbiota in Weaned Piglets.

Animals (Basel) 2022 Jul 7;12(14). Epub 2022 Jul 7.

Key Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China.

A total of 16 crossbred (Duroc × Landrace × Yorkshire) barrows, with an average initial body weight of 8.61 ± 0.24 kg (28 days of age), were randomly allotted into the control group (CON group) and high ambient temperature group (HT group) with 8 replicates per group, 1 pig per replicate. The ambient temperature of the CON group was controlled at 26 ± 1 °C, and the HT group was controlled at 35 ± 1 °C. The study lasted for 21 days. Our results showed that high ambient temperature significantly decreased the average daily feed intake (ADFI) and average daily gain (ADG) of piglets ( < 0.05), and the feed-to-gain ratio was significantly increased ( < 0.05). The liver index, spleen index, and thymus index of piglets in the HT group were significantly decreased ( < 0.05). The villous height (VH) of the duodenum, jejunum, and ileum of piglets in the HT group was significantly decreased ( < 0.05), whereas the crypt depth (CD) of the duodenum was significantly increased ( < 0.05), and the VH-to-CD ratio of the duodenum and ileum was significantly decreased ( < 0.05). The piglets in the HT group showed a higher ( < 0.05) observed-species index, PD whole tree index, and Shannon index, indicating that there was a significant difference in species richness and diversity between the two groups. At the genus level, the piglets in the HT group showed a greater ( < 0.05) percent of , , and . HT reduced glycan biosynthesis and metabolism, transport and catabolism, lipid metabolism, amino acids metabolism, secondary metabolites biosynthesis, aging, endocrine system, signaling molecules, and interaction of colon microbiota ( < 0.05), and increased signal transduction, cell motility, transcription, and genetic information processing ( < 0.05).
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http://dx.doi.org/10.3390/ani12141743DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312015PMC
July 2022

Ultrasound-guided transversus thoracic muscle plane-pectoral nerve block for postoperative analgesia after modified radical mastectomy: a comparison with the thoracic paravertebral nerve block.

Perioper Med (Lond) 2022 Jul 27;11(1):39. Epub 2022 Jul 27.

Department of Anesthesiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, No.100, Haining Road, Hong Kou District, Shanghai, 200080, China.

Background: Modified radical mastectomy (MRM) is the most effective and common type of invasive surgery for breast cancer. However, it causes moderate to severe acute pain and even lasts for a long postoperative period. Transversus thoracic muscle plane-pectoral nerve block (TTP-PECS) is a novel and promising interfacial plane block which can provide analgesia for MRM while thoracic paravertebral nerve block (TPVB) is also widely used for this purpose. This study compared the postoperative analgesia between the ultrasound-guided TTP-PECS and TPVB in patients undergoing MRM.

Methods: In this randomized controlled trial, eighty female breast cancer patients undergoing unilateral MRM with sentinel lymph node dissection (SLND) and axillary dissection (ALND) were enrolled. Patients were randomized to receive either ultrasound-guided TTP-PECS (TTP-PECS group, n = 40) or TPVB (TPVB group, n = 40) with 0.5% ropivacaine 30 ml. Evaluated variables included 24 h postoperative total PCA fentanyl consumption, including PCA background consumption and PCA press consumption (per bolus dosage multiply by the effective pressing times), and intraoperative fentanyl consumption, as well as postoperative flurbiprofen axetil requirement, duration of analgesia, blocking area, pain intensity at rest and during activity, ability to reduce the inflammatory response, and the quality of recovery 40 (QoR-40) score of patients.

Results: Compared with the TPVB, the main blocking area was T-T in the TTP-PECS group, which was more suitable for the MRM. TTP-PECS has a longer analgesia duration than TPVB; 24 h postoperative total PCA fentanyl consumption, especially the PCA press consumption, and the postoperative flurbiprofen axetil requirement were decreased in the TTP-PECS group than those in the TPVB group. Furthermore, the VAS scores at rest and during activity and inflammatory response were lower in the TTP-PECS group compared with the TPVB group at 12 h postoperatively. Finally, the total QoR-40 score, especially for the scores of pain; emotional state; and patient support were better in the TTP-PECS group.

Conclusion: Compared with the TPVB, TTP-PECS can provide better postoperative analgesia in patients undergoing MRM, simultaneously reduce the inflammatory response, and prompt early recovery. These results suggest that TTP-PECS is an attractive alternative to TPVB for postoperative analgesia of modified radical mastectomy.
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http://dx.doi.org/10.1186/s13741-022-00270-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9327269PMC
July 2022

Effect of different surgical positions on intraocular pressure: a cross-sectional study.

BMC Ophthalmol 2022 Jul 26;22(1):318. Epub 2022 Jul 26.

Department of Anesthesiology, China-Japan Friendship Hospital, Beijing, 100029, People's Republic of China.

Background: Intraoperative intraocular pressure (IOP) elevation is a risk factor for postoperative blindness. Surgical position is associated with intraoperative IOP elevation. In China, there are few studies on the effect of various surgical positions on intraoperative IOP. This study was conducted to explore IOP change and its related factors in four common surgical positions in China.

Methods: This was a cross-sectional observational study. A total of 325 surgical patients who had non-ocular surgery from January 2019 to December 2019 in the hospital, were enrolled in this study. During their surgeries for general anesthesia, these participants were placed in lithotomy position/lateral position/prone position/supine position according to their surgery requirement. IOP was measured by icareTA03 handheld portable tonometer at 9 different time points from admission to exiting the operation room. And general information, postural position, and surgery information were collected through a uniform questionnaire. Multivariate analysis was performed to explore the related factors of IOP change.

Results: IOP of both eyes on lithotomy position, lateral position, and supine position showed statistical differences by ANOVA test at each time point (p < 0.05). IOP of both eyes in the prone position before exit from the operating room was significantly higher than IOP 10-min after anesthesia (p < 0.01). IOP under different postural angles showed statistical differences (F value = 4.85, P < 0.05), and the larger the head-down angle, the higher the IOP. IOP on the compressed side in the lateral position was higher than that on the non-compressed side (p < 0.01). In the multivariate linear regression analysis adjusted by other factors, postural position and baseline IOP were associated with IOP difference between before and after surgery (p < 0.01).

Conclusion: IOP in the four surgical positions showed different change patterns with the surgical process and position change. Nurses should assist the surgeon to reduce the head-down angle without interfering with the surgical operation and strengthen the inspection of IOP on patients with long-time surgery, to avoid intraoperative rapid IOP changes.
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http://dx.doi.org/10.1186/s12886-022-02547-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317174PMC
July 2022

Extracellular vesicles engineered to bind albumin demonstrate extended circulation time and lymph node accumulation in mouse models.

J Extracell Vesicles 2022 Jul;11(7):e12248

Biomolecular Medicine, Clinical Research Center, Department of Laboratory Medicine Karolinska Institutet, Stockholm, Sweden.

Extracellular vesicles (EVs) have shown promise as potential therapeutics for the treatment of various diseases. However, their rapid clearance after administration could be a limitation in certain therapeutic settings. To solve this, an engineering strategy is employed to decorate albumin onto the surface of the EVs through surface display of albumin binding domains (ABDs). ABDs were either included in the extracellular loops of select EV-enriched tetraspanins (CD63, CD9 and CD81) or directly fused to the extracellular terminal of single transmembrane EV-sorting domains, such as Lamp2B. These engineered EVs exert robust binding capacity to human serum albumins (HSA) in vitro and mouse serum albumins (MSA) after injection in mice. By binding to MSA, circulating time of EVs dramatically increases after different routes of injection in different strains of mice. Moreover, these engineered EVs show considerable lymph node (LN) and solid tumour accumulation, which can be utilized when using EVs for immunomodulation, cancer- and/or immunotherapy. The increased circulation time of EVs may also be important when combined with tissue-specific targeting ligands and could provide significant benefit for their therapeutic use in a variety of disease indications.
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http://dx.doi.org/10.1002/jev2.12248DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9314316PMC
July 2022

Inhibitor formation and detoxification during lignocellulose biorefinery: A review.

Bioresour Technol 2022 Jul 22;361:127666. Epub 2022 Jul 22.

Department of Mechanical Engineering, City University of Hong Kong, Kowloon Tong, Hong Kong; Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-li 32003, Taiwan. Electronic address:

For lignocellulose biorefinery, pretreatment is needed to maximize the cellulose accessibility, frequently generating excess inhibitory substances to decline the efficiency of the subsequent fermentation processes. This mini-review updates the current research efforts to detoxify the adverse impacts of generated inhibitors on the performance of biomass biorefinery. The lignocellulose pretreatment processes are first reviewed. The generation of inhibitors, furans, furfural, phenols, formic acid, and acetic acid, from the lignocellulose, with their action mechanisms, are listed. Then the detoxification processes are reviewed, from which the biological detoxification processes are noted as promising and worth further study. The challenges and prospects for applying biological detoxification in lignocellulose biorefinery are outlined. Integrated studies considering the entire biorefinery should be performed on a case-by-case basis.
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http://dx.doi.org/10.1016/j.biortech.2022.127666DOI Listing
July 2022

The comparative effects of sacubitril/valsartan versus enalapril on pulmonary hypertension due to heart failure with a reduced ejection fraction.

Pulm Circ 2022 Jul 1;12(3):e12034. Epub 2022 Jul 1.

Department of Cardiology Handan Central Hospital Handan Hebei China.

The purpose of this study was to investigate the effects of sacubitril/valsartan on right ventricular (RV) function in patients with pulmonary hypertension (PH) due to heart failure with reduced ejection fraction (HFrEF). We prospectively enrolled patients with HFrEF-induced PH admitted to the Department of Cardiology between August 2018 and December 2019. Patients were randomized to receive oral treatment with sacubitril/valsartan or enalapril. Epidemiological data were recorded before treatment. Echocardiography was performed at admission and 6 months of follow-up, and all parameters were compared. Major adverse cardiac events (MACEs) were compared between baseline and 6 months follow-up. There were no significant differences in the baseline characteristics between the two groups. After 6 months of treatment, both treatment groups improved the following parameters from baseline (mean ± SD): left atrium, left ventricle, the left ventricular ejection function (LVEF), RV systolic function (the tricuspid annular plane systolic excursion [TAPSE], the systolic pulmonary artery pressure [sPAP], and TAPSE/sPAP). After 6 months, sacubitril/valsartan improved significantly the following parameters compared with enalapril (all  < 0.05): LVEF (47.07 ± 6.93% vs. 43.47 ± 7.95%); TAPSE (15.33 ± 1.31 vs. 14.78 ± 1.36 mm); sPAP (36.76 ± 14.32 vs. 42.26 ± 12.07 mmHg); and TAPSE/sPAP ratio (0.50 ± 0.23 vs. 0.39 ± 0.14), respectively. There was no difference in readmissions due to recurrent heart failure. Sacubitril/valsartan seems to provide more beneficial effects among patients with HFrEF-induced PH to improve RV function, along with a decrease in pulmonary pressure.
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http://dx.doi.org/10.1002/pul2.12034DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297686PMC
July 2022

Single-Cell Transcriptomics of Immune Cells Reveal Diversity and Exhaustion Signatures in Non-Small-Cell Lung Cancer.

Front Immunol 2022 6;13:854724. Epub 2022 Jul 6.

Laboratory of Omics Technology and Bioinformatics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.

Understanding immune cell phenotypes in the tumor microenvironment (TME) is essential for explaining and predicting progression of non-small cell lung cancer (NSCLC) and its response to immunotherapy. Here we describe the single-cell transcriptomics of CD45 immune cells from tumors, normal tissues and blood of NSCLC patients. We identified three clusters of immune cells exerting immunosuppressive effects: CD8 T cells with exhausted phenotype, tumor-associated macrophages (TAMs) with a pro-inflammatory M2 phenotype, and regulatory B cells (B regs) with tumor-promoting characteristics. We identified genes that may be mediating T cell phenotypes, including the transcription factors ONECUT2 and ETV4 in exhausted CD8 T cells, TIGIT and CTL4 high expression in regulatory T cells. Our results highlight the heterogeneity of CD45 immune cells in the TME and provide testable hypotheses about the cell types and genes that define the TME.
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http://dx.doi.org/10.3389/fimmu.2022.854724DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9299430PMC
July 2022

Ameliorates SD-Induced Stress Responses and Gut Dysbiosis by Increasing the Absorption of Gut-Derived GABA in Rhesus Monkeys.

Front Immunol 2022 7;13:915393. Epub 2022 Jul 7.

Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Sleep deprivation (SD) has become a health problem in the modern society. Although probiotics supplementation has been proven to improve SD-induced gut dysbiosis, the potential neuroendocrine mechanisms remain elusive. In this study, thirty rhesus monkeys (RMs) were recruited. Paradoxical sleep, bright light, and noise were used to build an RM SD model. We examined the plasma γ-aminobutyric acid (GABA), stress hormones, and inflammatory cytokines using ELISAs. 16S ribosomal DNA sequencing and untargeted metabolomics sequencing were employed to detect gut microbial community and metabolites, respectively. The results of our study showed that RMs subjected to SD had elevated plasma stress hormones (such as cortisol and norepinephrine) and proinflammatory cytokines (such as TNF-α, IL-6, and IL-8), and a decreased anti-inflammatory cytokine IL-10 level. Additionally, SD could give rise to a significant change in gut microbiota and metabolites. The differential gut microbiota and metabolites caused by SD were enriched in the signaling pathways related to GABA metabolism. Pearson correlation analysis revealed that there is a significant correlation between plasma GABA and SD-induced stress responses and gut dysbiosis. The supplementation of GABA-producing probiotics could significantly increase the relative abundance of and plasma GABA levels, and reverse SD-induced stress responses and gut dysbiosis. Therefore, we speculated that SD-induced stress response and gut dysbiosis might be an outcome of reduced gut-derived GABA absorption. The supplementation of GABA-producing might be beneficial for the treatment of SD-induced intestinal dysfunction.
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http://dx.doi.org/10.3389/fimmu.2022.915393DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302489PMC
July 2022

Acetylation of p62 regulates base excision repair through interaction with APE1.

Cell Rep 2022 Jul;40(3):111116

Beijing Key Laboratory of Protein Posttranslational Modifications and Cell Function, Department of Biochemistry and Biophysics, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China. Electronic address:

p62, a well-known adaptor of autophagy, plays multiple functions in response to various stresses. Here, we report a function for p62 in base excision repair that is distinct from its known functions. Loss of p62 impairs base excision repair capacity and increases the sensitivity of cancer cells to alkylating and oxidizing agents. In response to alkylative and oxidative damage, p62 is accumulated in the nucleus,acetylated by hMOF,and deacetylated by SIRT7, and acetylated p62 is recruited to chromatin. The chromatin-enriched p62 directly interacts with APE1, a key enzyme of the BER pathway, and promotes its endonuclease activity, which facilitates BER and cell survival. Collectively, our findings demonstrate that p62 is a regulator of BER and provide further rationale for targeting p62 as a cancer therapeutic strategy.
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http://dx.doi.org/10.1016/j.celrep.2022.111116DOI Listing
July 2022
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