Publications by authors named "Ying Wang"

9,097 Publications

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plays an oncogene-like role in glioma through promoting proliferation and inhibiting apoptosis.

Ann Transl Med 2021 Apr;9(7):542

Department of Pathology, School of Basic Medical Science, Central South University, Changsha, China.

Background: Uridine diphosphate--acetylglucosamine pyrophosphorylase-1-like-1 () is involved in protein glycosylation and promotes proliferation in some tumors. By analyzing the publicly available Gene Expression Omnibus (GEO) database, we found that displayed a significant change between paired glioma and normal brain tissues. The purpose of this study was to investigate the expression and functional role of in glioma.

Methods: To determine the expression level of in glioma, immunohistochemistry (IHC) staining was performed in tissue microarrays of 160 gliomas and 24 normal brain tissues. The correlation between expression and the outcomes of glioma patients was analyzed. Human glioblastoma cell lines, U251 and U87, were employed in this study. Endogenous expression in U251 and U87 cells was detected by quantitative real-time polymerase chain reaction (qRT-PCR). A lentiviral short hairpin RNA (shRNA) vector (shUAP1L1) was constructed and used to infect U251 and U87 cells to knock down the expression of . We performed 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, colony formation assay, flow cytometry, human apoptosis antibody array, and in vivo subcutaneous xenograft model to investigate the biological functions of .

Results: We revealed that expression was obviously upregulated in the glioma tissues. The increased expression level was clinically associated with higher tumor grades and poorer patient prognoses. Moreover, we demonstrated that knockdown suppressed proliferation and increased apoptosis of glioma cells in vitro. In the xenograft mouse model, we further verified that knockdown could attenuate the growth of glioma cells in vivo.

Conclusions: These results indicated that may play an oncogene-like role in glioma, especially in high grade glioma, and thus may be of clinical importance as a future therapeutic target.
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http://dx.doi.org/10.21037/atm-20-2809DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105798PMC
April 2021

Cell-specific transcriptional control of mitochondrial metabolism by TIF1γ drives erythropoiesis.

Science 2021 May;372(6543):716-721

Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 01238, USA.

Transcription and metabolism both influence cell function, but dedicated transcriptional control of metabolic pathways that regulate cell fate has rarely been defined. We discovered, using a chemical suppressor screen, that inhibition of the pyrimidine biosynthesis enzyme dihydroorotate dehydrogenase (DHODH) rescues erythroid differentiation in bloodless zebrafish mutant embryos defective for transcriptional intermediary factor 1 gamma (). This rescue depends on the functional link of DHODH to mitochondrial respiration. The transcription elongation factor TIF1γ directly controls coenzyme Q (CoQ) synthesis gene expression. Upon loss, CoQ levels are reduced, and a high succinate/α-ketoglutarate ratio leads to increased histone methylation. A CoQ analog rescues 's bloodless phenotype. These results demonstrate that mitochondrial metabolism is a key output of a lineage transcription factor that drives cell fate decisions in the early blood lineage.
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http://dx.doi.org/10.1126/science.aaz2740DOI Listing
May 2021

Higher mortality in lung cancer patients with COVID-19? A systematic review and meta-analysis.

Lung Cancer 2021 May 5. Epub 2021 May 5.

Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing University Cancer Hospital, Chongqing, 400030, China. Electronic address:

Objective: Given that the coronavirus disease 2019 (COVID-19) mainly spreads through the respiratory system and is associated with severe pulmonary complications, lung cancer patients may have worse outcomes than those with other tumors. There is no confirmed evidence about the mortality comparison between COVID-19 patients with lung cancer and other tumors. We performed a systematic review and pooled analysis to provide precise estimates of the mortality rate of COVID-19 patients with lung cancer and other tumors.

Materials And Methods: Our study systemically included and reviewed 13 studies on the characteristics of COVID-19 patients with lung cancer published up to November 1, 2020. The primary endpoint was all-cause mortality. We also compared the all-cause mortality rates in China and other regions as a secondary endpoint. The mortality rate was assessed with a fixed-effects model, which was used to derive the pooled mortality and 95 % confidence interval (CI).

Results: Thirteen studies from different countries, involving 1,229 patients with both COVID-19 and cancer, were selected for the pooled analysis. A total of 343 deaths were recorded in this population: 86 for lung cancers and 257 for other tumors. The mortality rate varies from 18 % to 60 % for patients with lung cancer and COVID-19 and 10%-41% for other tumor patients with COVID-19. The overall meta-analysis did not show a significant mortality difference for the lung cancer and other tumor subgroups (OR = 1.47, 95 %CI = 0.98-2.20, p = 0.06, I = 23 %). Nevertheless, in regions other than China, the pooled mortality of lung cancer patients with COVID-19 was 42 %, which was significantly higher than that of other tumors (24 %) (OR = 2.73, 95 % CI = 1.54-4.86, p = 0.0006, I = 16 %).

Conclusion: Appropriate and aggressive preventive measures should be implemented to reduce the risk of COVID-19 in patients with cancer and optimally manage those who contract the infection.
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http://dx.doi.org/10.1016/j.lungcan.2021.05.002DOI Listing
May 2021

Emerging progress on diagnosis and treatment of female genital tuberculosis.

J Int Med Res 2021 May;49(5):3000605211014999

Department of Respiratory Medicine, Jingzhou Chest Hospital, Jingzhou, Hubei, China.

Female genital tuberculosis (FGTB) is an infection caused by and usually occurs secondary to pulmonary tuberculosis (TB) through the blood circulation, lymph circulation, or direct spreading from abdominal TB. FGTB is an uncommon type of TB that can destroy genital organs, and lead to menstrual disorders and infertility. The diagnosis of FGTB is often made by detection of acid-fast bacilli under microscopy, culture with endometrial biopsy, or histopathological examination of epithelioid granuloma on a biopsy. A multidrug anti-TB regimen is the major management of FGTB, including rifampicin, isoniazid, pyrazinamide, and ethambutol, while surgery is proposed in more deteriorated cases. However, the conception rate in infertile women with FGTB is still low, even after multidrug anti-TB therapy. Additionally, the risk of complications, such as ectopic pregnancy or miscarriage, remains high. In this review, we summarize the characteristics of FGTB, present current epidemiological data, and focus on its early diagnosis and effective management.
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http://dx.doi.org/10.1177/03000605211014999DOI Listing
May 2021

Remote Regioselective Radical C-H Functionalization of Unactivated C-H Bonds in Amides: The Synthesis of -Difluoroalkenes.

Org Lett 2021 May 13. Epub 2021 May 13.

College of Chemistry and Materials Science, Sichuan Normal University, Chengdu 610068, China.

The site-selective functionalization of unactivated aliphatic amines is an attractive and challenging synthetic approach. We herein report a general strategy for the remote site-selective functionalization of unactivated C(sp)-H bonds in amides by photogenerated amidyl radicals to form -difluoroalkenes with trifluoromethyl-substituted alkenes. The site selectivity is controlled by a 1,5-hydrogen atom transfer (HAT) process of the amide. This photocatalyzed transformation shows both chemo- and site-selectivity, facilitating the formation of a secondary, tertiary, or quaternary carbon center.
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http://dx.doi.org/10.1021/acs.orglett.1c01385DOI Listing
May 2021

A near-infrared light-excitable immunomodulating nano-photosensitizer for effective photoimmunotherapy.

Biomater Sci 2021 May 13. Epub 2021 May 13.

Key Laboratory of Functional Polymer Materials of Ministry of Education, College of Chemistry, Nankai University; State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, China.

Photodynamic therapy has great potential for tumor ablation and the activation of antitumor immune responses. However, its overall therapeutic efficiency is often limited by the immunosuppressive tumor microenvironment. We developed a near-infrared light-excitable immunomodulating nano-photosensitizer (NeINP) that can improve reactive oxygen species production and regulate the immunosuppressive TME to improve photoimmunotherapy. The NeINP is composed of a photosensitive core and a pH-responsive polymer shell, which allows for NeINP loading and delivery of small-molecular immunomodulators to tumor sites for regulation of the immunosuppressive TME and effective photoimmunotherapy. Through the co-delivery of celecoxib and the NIR-triggered photodynamic core to tumors, the NeINP was shown to regulate the immunosuppressive TME and enhance antitumor immunity, leading to the elimination of residual tumor and reduction of metastasis and recurrence. The NeINP can be optimized to co-deliver other immunomodulators, and thus has potential as a universal platform for efficient, precise photoimmunotherapy.
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http://dx.doi.org/10.1039/d1bm00569cDOI Listing
May 2021

Corrigendum: MR-guided 125I seed implantation treatment for maxillofacial malignant tumor.

J Appl Clin Med Phys 2021 May 12. Epub 2021 May 12.

Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

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http://dx.doi.org/10.1002/acm2.13272DOI Listing
May 2021

Application of blood microsampling in cynomolgus monkey and demonstration of equivalent monoclonal antibody PK parameters compared to conventional sampling.

Pharm Res 2021 May 12. Epub 2021 May 12.

Sana Biotechnology, 100 Technology Square, Cambridge, Massachusetts, 02139, USA.

Purpose: The purpose of this study was to evaluate the suitability of whole blood microsampling procedures in non-human primate (NHP) to support toxicokinetic assessments of biotherapeutics in non-human primates.

Method: A one-month single dose intravenous pharmacokinetic (PK) study was performed in male cynomolgus monkeys with a human IgG1 control monoclonal antibody (mAb) as a surrogate monoclonal antibody biotherapeutic. In this study, both serum samples (conventional sample collection) and microsampling samples were collected. Microsampling samples were collected from two sites on cynomolgus monkey, with each site using two different devices for the whole blood collection. The drug concentrations from all sample types were determined using a quantitative ligand binding assay (LBA). The PK parameters obtained from microsampling samples and serum samples were examined using a standard PK analysis method. The comparability of key PK parameters from both sample types were analyzed statistically.

Results: Similar profiles of drug concentrations versus timepoints from all sampling procedures were observed. The correlations of PK concentration data obtained from serum and microsampling samples were ≥ 0.97 using Brand Alman Plot analysis. The key PK parameters obtained from microsampling samples were comparable to those obtained from serum samples (the % differences of mean PK parameters obtained from both sample types were within ±25%).

Conclusion: This study confirmed that PK parameters obtained from samples using microsampling were comparable to that of serum samples in cynomolgus monkeys. Therefore, the microsampling procedure described can be used as a substitute for conventional sampling procedure to support PK/TK studies of biotherapeutics in non-clinical product developments.
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http://dx.doi.org/10.1007/s11095-021-03044-6DOI Listing
May 2021

Interfering microRNA-410 attenuates atherosclerosis via the HDAC1/KLF5/IKBα/NF-κB axis.

Mol Ther Nucleic Acids 2021 Jun 13;24:646-657. Epub 2021 Mar 13.

Department of Ear-Nose-Throat, The Second Hospital of Jilin University, Changchun 130041, Jilin Province, PR China.

MicroRNA (miR)-410 plays a potential role in the pathogenesis of atherosclerosis. The current study mainly focuses on the underlying mechanism of miR-410/histone deacetylase 1 (HDAC1)/KLF5/nuclear factor κB (NF-κB) inhibitor α (IKBα)/NF-κB axis in atherosclerosis. miR-410 expression was determined using quantitative real-time PCR in both mouse models of atherosclerosis and human umbilical endothelial cells (HUVECs) treated with oxidized low-density lipoprotein (ox-LDL). The study subsequently predicted regulators associated with miR-410 through bioinformatics, and their binding relation was further verified through dual luciferase reporter gene and RNA immunoprecipitation (RIP) assays, and how HDAC1 regulated KLF5 was tested through coimmunoprecipitation (coIP). In HUVECs, miR-410 and HDAC1 mRNA expression; HDAC1, KLF5, IKBα, p65, p-p65, VCAM-1, ICAM-1, and MCP-1 protein expression; and inflammatory cytokine expressions were detected using quantitative real-time PCR, western blot, and ELISA. The present study further tested cell functions by Cell Counting Kit-8 (CCK-8), flow cytometry, and the colony-formation assay. It was revealed that miR-410 could target HDAC1, whereas HDAC1 could target transcription factor KLF5, increasing IKBα expression, thus suppressing NF-κB in atherosclerosis. Furthermore, silencing miR-410 or overexpressing HDAC1 increased cell viability and suppressed apoptosis and an inflammatory reaction in HUVECs in atherosclerosis. Blocking miR-410 promotes HDAC1 expression and increases IKBα levels through KLF5 to suppress NF-κB, thus preventing development of atherosclerosis.
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http://dx.doi.org/10.1016/j.omtn.2021.03.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076652PMC
June 2021

Syncytia formation during SARS-CoV-2 lung infection: a disastrous unity to eliminate lymphocytes.

Cell Death Differ 2021 May 12. Epub 2021 May 12.

CAS Key Laboratory of Tissue Microenvironment and Tumor, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.

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http://dx.doi.org/10.1038/s41418-021-00795-yDOI Listing
May 2021

Acute psychological impact of coronavirus disease 2019 outbreak among psychiatric professionals in China: a multicentre, cross-sectional, web-based study.

BMJ Open 2021 05 11;11(5):e047828. Epub 2021 May 11.

Department of Psychiatry, Renmin Hospital of Wuhan University, Wuhan, China

Objectives: To assess the magnitude of mental health outcomes and associated factors among psychiatric professionals in mental health services during COVID-19 in China.

Design, Setting And Participants: This cross-sectional, survey-based, region-stratified study collected demographic data and mental health measurements from psychiatric professionals in 34 hospitals between 29 January and 7 February 2020, in China. Hospitals equipped with fever clinics or deployed on wards for patients with COVID-19 were eligible.

Primary Outcome And Measures: The severity of symptoms of depression, anxiety, insomnia and distress were assessed by the Chinese versions of 9-item Patient Health Questionnaire, 7-item Generalised Anxiety Disorder, 7-item Insomnia Severity Index and 22-item Impact of Event Scale-Revised, respectively. Multivariable logistic regression and structural equation modelling was performed to identify factors associated with mental health outcomes.

Results: A total of 610 psychiatric professionals were included. 29.8% were employed in Wuhan, and 22.5% were frontline workers. A considerable proportion of participants reported symptoms of depression (461 (75.6%)), anxiety (282 (46.2%)), insomnia (336 (55.1%)) and mental stress (481 (78.9%)). Psychiatric symptoms were associated with worrying about infection (eg, OR 2.36 (95% CI 1.27 to 4.39) for anxiety), risks of exposure to COVID-19 (eg, having inadequate personal protection equipment, OR 2.43 (1.32 to 4.47) for depression) and self-perceived physical health (eg, OR 3.22 (2.24 to 4.64) for mental stress). Information sources of COVID-19 were also found to be both positively (eg, information from relatives, OR 2.16 (1.46 to 3.21) for mental stress) and negatively (eg, information from TV, OR 0.52 (0.35 to 0.77) for mental stress) associated with mental stress. There is preliminary evidence that mental health might benefit from greater availability of mental healthcare services. The structural equation model analysis indicated that worrying about infection may be the primary mediator via which risk of exposure to COVID-19 pandemic affects the mental health of psychiatric professionals.

Conclusions: The current findings demonstrate several pathways via which the COVID-19 pandemic may have negatively affected the mental health of psychiatric professionals in China.
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http://dx.doi.org/10.1136/bmjopen-2020-047828DOI Listing
May 2021

Maternal Exposure to Pesticides and Risk of Autism Spectrum Disorders in Offspring: A Meta-analysis.

J Autism Dev Disord 2021 May 12. Epub 2021 May 12.

Mental Health Center, Renmin Hospital of Wuhan University, Jiefang Road 238#, Wuchang District, Wuhan, 430060, People's Republic of China.

This meta-analysis was conducted to estimate the overall association between maternal exposure to pesticides and risk of ASD in offspring. PubMed, EMBASE, Web of Science, and the PsycINFO were searched until December 30, 2020 to include eligible studies. Eight studies with 50,426 participants, 5810 of whom had ASD, were involved in the study. Overall, the summary OR (95% confidence interval) of ASDs in offspring for maternal exposure to pesticide estimated by residential proximity measures and self-report was 1.88 (1.10-3.20). However, maternal exposure to pesticide measured by biomarkers was not associated with an increased risk of ASDs (pooled OR 1.13; 95% CI 0.83-1.54). Further well-designed studies are needed to confirm our findings.
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http://dx.doi.org/10.1007/s10803-021-05063-zDOI Listing
May 2021

A randomized controlled study on an integrated approach to prevent and treat re-adhesion after transcervical resection of moderate-to-severe intrauterine adhesions.

Clinics (Sao Paulo) 2021 5;76:e1987. Epub 2021 May 5.

Department of Obstetrics and Gynecology, Nanping People's Hospital to Fujian University of Traditional Chinese Medicine, Nanping 353000, Fujian, China.

Objectives: This study aims to compare the clinical efficacy of an integrated approach to prevent and treat the recurrence of moderate-to-severe intrauterine adhesions (IUA) after hysteroscopic transcervical resection of adhesion (TCRA).

Methods: The study included a total of 70 patients with moderate-to-severe IUAs who underwent TCRA. Patients were randomly divided into two groups: treatment group (n=35) and control group n=35). In the treatment group, patients underwent balloon uterine stent placement and artificial cycle as well as received intrauterine perfusion of Danshen injection and oral Chinese medicine. In the control group, patients underwent balloon uterine stent placement and artificial cycle as well as received hyaluronic acid sodium and intrauterine device (IUD). Follow-up was performed after treatment of uterine cavity, menstruation and pregnancy.

Results: After 3 months of treatment, we observed a significantly lower rate of intrauterine re-adhesion (45.71% versus 77.14%, p=0.044) and significantly higher clinical efficiency (82.86% versus 77.14%, p=0.025) in the treatment group than those in the control group. After 6 months of treatment, we observed a significantly higher clinical efficiency in the treatment group than that in the control group (88.57% versus 68.57%, p=0.039). During the follow-up period, the pregnancy rate was 45.71% and 37.14% in the treatment group and control group, respectively, although the difference was not statistically significant (p=0.628).

Conclusions: After surgical management of IUA, the integrated treatment combining a uterus stent placement and artificial cycle with Danshen injection and oral Chinese medicine can improve the condition of menstruation, and prevent and treat recurrence of IUA.
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http://dx.doi.org/10.6061/clinics/2021/e1987DOI Listing
May 2021

MAT2: Manifold alignment of single-cell transcriptomes with cell triplets.

Bioinformatics 2021 May 10. Epub 2021 May 10.

Institute of Science and Technology for Brain-Inspired Intelligence, Fudan University, Shanghai 200433, China.

Motivation: Aligning single-cell transcriptomes is important for the joint analysis of multiple single-cell RNA sequencing datasets, which in turn is vital to establishing a holistic cellular landscape of certain biological processes. Although numbers of approaches have been proposed for this problem, most of which only consider mutual neighbors when aligning the cells without taking into account known cell type annotations.

Results: In this work, we present MAT2 that aligns cells in the manifold space with a deep neural network employing contrastive learning strategy. Compared with other manifold-based approaches, MAT2 has two-fold advantages. Firstly, with cell triplets defined based on known cell type annotations, the consensus manifold yielded by the alignment procedure is more robust especially for datasets with limited common cell types. Secondly, the batch-effect-free gene expression reconstructed by MAT2 can better help annotate cell types. Benchmarking results on real scRNA-seq datasets demonstrate that MAT2 outperforms existing popular methods. Moreover, with MAT2, the hematopoietic stem cells are found to differentiate at different paces between human and mouse.

Availability And Implementation: MAT2 is publicly available at https://github.com/Zhang-Jinglong/MAT2.

Supplementary Information: Supplementary data are available at Bioinformatics online.
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http://dx.doi.org/10.1093/bioinformatics/btab250DOI Listing
May 2021

MAT2: Manifold alignment of single-cell transcriptomes with cell triplets.

Bioinformatics 2021 May 11. Epub 2021 May 11.

Institute of Science and Technology for Brain-Inspired Intelligence, Fudan University, Shanghai 200433, China.

Motivation: Aligning single-cell transcriptomes is important for the joint analysis of multiple single-cell RNA sequencing datasets, which in turn is vital to establishing a holistic cellular landscape of certain biological processes. Although numbers of approaches have been proposed for this problem, most of which only consider mutual neighbors when aligning the cells without taking into account known cell type annotations.

Results: In this work, we present MAT2 that aligns cells in the manifold space with a deep neural network employing contrastive learning strategy. Compared with other manifold-based approaches, MAT2 has two-fold advantages. Firstly, with cell triplets defined based on known cell type annotations, the consensus manifold yielded by the alignment procedure is more robust especially for datasets with limited common cell types. Secondly, the batch-effect-free gene expression reconstructed by MAT2 can better help annotate cell types. Benchmarking results on real scRNA-seq datasets demonstrate that MAT2 outperforms existing popular methods. Moreover, with MAT2, the hematopoietic stem cells are found to differentiate at different paces between human and mouse.

Availability And Implementation: MAT2 is publicly available at https://github.com/Zhang-Jinglong/MAT2.

Supplementary Information: Supplementary data are available at Bioinformatics online.
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http://dx.doi.org/10.1093/bioinformatics/btab260DOI Listing
May 2021

Lenalidomide downregulates ACE2 protein abundance to alleviate infection by SARS-CoV-2 spike protein conditioned pseudoviruses.

Signal Transduct Target Ther 2021 05 10;6(1):182. Epub 2021 May 10.

Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

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http://dx.doi.org/10.1038/s41392-021-00608-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107201PMC
May 2021

Structural and functional characteristics of plant PHD domain-containing proteins.

Yi Chuan 2021 Apr;43(4):323-339

Institute of Plant Science, College of Life Science, Fudan University, Shanghai 200433, China.

Plant homeodomain (PHD) is a class of transcription factor in the Zinc finger domain family. The most important function of which is to recognize various histone modifications, including histone methylation and acetylation, etc. They can also bind to DNA. Proteins with PHD domains, some of which possess histone modification enzyme activity, or can interact with histone modification enzymes, and some are associated with DNA methylation, with E3 ubiquitin ligase activity, or even can be chromatin remodeling factors. As transcriptional regulators, they play an important role in plant growth and development. In this review, we summarize the structural features and substrate binding specificity of PHD domains (including H3K4me3/0, H3K9me3, H3R2, H3K14ac) and DNA, the conservation of plant PHD domain in evolution, the molecular mechanism of known PHD domain-containing proteins in plants, providing a reference for further understanding of the involvement of these proteins during plant growth and development.
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http://dx.doi.org/10.16288/j.yczz.20-412DOI Listing
April 2021

Ultra-high mechanical property and multi-layer porous structure of amidoximation ethylene-acrylic acid copolymer balls for efficient and selective uranium adsorption from radioactive wastewater.

Chemosphere 2021 Apr 30;280:130722. Epub 2021 Apr 30.

Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Materials Science and Chemical Engineering, Harbin Engineering University, Harbin, 150001, China; Institute of Advanced Marine Materials, Harbin Engineering University, 150001, China. Electronic address:

Adsorption uranium [U(VI)] from U-containing radioactive wastewater (URW) is a critical strategy for solving the resource shortage and environmental pollution in pace with the sustainable development of nuclear energy. However, the URW universally exhibits acidity and contains co-existing metal ions with high concentration. Herein, the amidoximation ethylene-acrylic acid copolymer balls (EAA-AO) with aciduric and super-high mechanical property were successfully synthesized through grafting diaminomaleonitrile and further treatment of amidoximation. Significantly, the mechanical properties of EAA-AO were not affected by the grafting process and maintained super-high mechanical properties. Furthermore, the -NH and unreacted -CN groups in diaminomaleonitrile adjusted the pK to make the optimal pH be 4. In addition, the microstructure of EAA-AO was transformed from the original dense to multi-layer porous structure, which promoted the mass transfer process and the contact between uranyl ions (UO) and internal adsorption active sites. The adsorption capacity of EAA-AO was about 1.78 times that of EAA at pH = 4, and the adsorption capacity for U(VI) was about 8.17 times that of Ba with the second highest adsorption capacity. Therefore, the EAA-AO exhibited ultra-high adsorption performance (q = 3.196 mg g) in the artificial radioactive wastewater, laying a good foundation for subsequent large-scale industrial adsorption of U(VI) in nuclear industrial wastewater.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130722DOI Listing
April 2021

Genome-wide characterization of bZIP transcription factors and their expression patterns in response to drought and salinity stress in Jatropha curcas.

Int J Biol Macromol 2021 May 7. Epub 2021 May 7.

Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China. Electronic address:

The basic leucine zipper (bZIP) family is one of the largest families of transcription factors (TFs) in plants and is responsible for various functions, including regulating development and responses to abiotic/biotic stresses. However, the roles of bZIPs in the regulation of responses to drought stress and salinity stress remain poorly understood in Jatropha curcas L., a biodiesel crop. In the present study, 50 JcbZIP genes were identified and classified into nine groups. Cis-element analysis indicated that JcbZIP genes are associated with abiotic stress. Gene expression patterns and quantitative real-time PCR (qRT-PCR) showed that four JcbZIP genes (JcbZIPs 34, 36, 49 and 50) are key resistance-related genes under both drought and salinity stress conditions. On the basis of the results of cis-element and phylogenetic analyses, JcbZIP49 is most likely involved in responses to drought and salinity stress; moreover, JcbZIP34 and JcbZIP36 might also play important roles in seed development and response to abiotic stress. These findings advance our understanding of the comprehensive characteristics of JcbZIP genes and provide new insights for functional validation in the further.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.05.027DOI Listing
May 2021

Copper-Catalyzed Chemoselective Silylative Cyclization of 2,2'-Diethynylbiaryl Derivatives.

Org Lett 2021 May 10. Epub 2021 May 10.

Hefei National Laboratory for Physical Sciences at the Microscale, and Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, P. R. China.

In this protocol, copper-catalyzed diverse silylative carbocyclization reactions of 2,2'-diethynylbiaryl derivatives with silaboronate were reported. Three new and novel types of domino reactions for the copper-catalyzed transformation of silaboronate were discovered. The corresponding cyclobuta[]phenanthrene, bis((silyl)methyl)phenanthrene, and silyl-substituted exocyclic diene products were chemoselectively formed with high efficiency.
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http://dx.doi.org/10.1021/acs.orglett.1c00968DOI Listing
May 2021

Comprehensive analysis of carbon emissions, economic growth, and employment from the perspective of industrial restructuring: a case study of China.

Environ Sci Pollut Res Int 2021 May 10. Epub 2021 May 10.

Key Laboratory of Ocean Energy Utilization and Energy Conservation of Ministry of Education, School of Energy and Power Engineering, Dalian University of Technology, No.2 Linggong Road, High-Tech Pack, Dalian, 116024, Liaoning, China.

Industrial restructuring is a significant measure for low-carbon transition. In principle, carbon emissions can be effectively reduced by limiting the output of high-emission sectors; however, the socio-economic effects of the sectors should also be considered. Moreover, owing to the limitations of the method or data, the interactions between households and production sectors have been neglected in the study of industrial restructuring, resulting in an incomplete and potentially biased understanding of the role of households. To fill this gap, we applied a semi-closed input-output model to identify key sectors by economic and emission linkages and measure the employment impacts (direct, indirect, and induced) of reduced carbon emissions. The empirical results for China in 2010-2018 showed that relatively small changes in key emission sectors would significantly affect the economic growth, and reduced carbon emissions reduction would generally lead to high job losses. Promoting labor-intensive sectors, particularly the service sector, is conducive to achieving a "multi-win" situation for economic development, carbon emission reductions, and stable employment. Furthermore, our results highlight the significance of households: expanding consumption and increasing household income can bring multiple benefits, such as economic growth, job creation, and low carbon emissions. These findings can provide useful information for identifying the optimized path of restructuring and helping achieve the sustainable development of the environment, economy, and society.
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http://dx.doi.org/10.1007/s11356-021-14040-zDOI Listing
May 2021

Sclerosing polycystic adenosis of the submandibular gland: Two case reports.

World J Clin Cases 2021 Apr;9(12):2930-2936

Department of Stomatology, Tongji Hospital of Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430000, Hubei Province, China.

Background: Sclerosing polycystic adenosis (SPA) is a rare disease of salivary glands, similar to fibrocystic disease of the breast. It occurs over a wide age range and exhibits a slight female preference. Most SPA cases have occurred in the parotid gland. The exact nature of SPA is unclear, but its tumor nature has recently been proposed. Although SPA has a good prognosis after adequate surgery, atypical lesions might occur, ranging from mild dysplasia to carcinoma in some cases. To the best of our knowledge, only five cases of SPA in the submandibular gland have been reported to date. Here, we present two new cases of SPA involving the submandibular gland.

Case Summary: A 50-year-old woman and a 52-year-old woman were referred to Tongji Hospital in Wuhan, China, with complaints of moderate pain, recurrent swelling, and a mass in the submandibular area. After admission, the two cases of the submandibular mass were examined physically. The boundary of the submandibular tumor was clear, and the range of motion was good. After preoperative examinations, surgery was performed on a selective basis. Postoperative histopathological examination revealed a well-defined mass with acinar structures, ducts, or cystic dilated glands of various sizes scattered in a large number of proliferative sclerosing stroma. There were flat and cuboidal cells, and eosinophils in the duct epithelium. There was also a eosinophilic substance in the lumen of dilated cysts. No atypical epithelial hyperplasia, invasive growth, or carcinoma was found. Based on the above findings, the mass was diagnosed as SPA. Both patients have remained asymptomatic and no recurrence or distant metastasis had occurred by the 7-mo and 5-year follow-up, respectively.

Conclusion: SPA is a rare disease of the salivary gland. Even though it has a good prognosis after adequate surgery, atypical lesions may occur from mild dysplasia to carcinoma . However, no recurrence, distant metastasis, or mortality has been reported for submandibular gland SPA. Clinicians and pathologists should be familiar with the characteristics of SPA in the submandibular gland to avoid misdiagnosis and overtreatment.
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http://dx.doi.org/10.12998/wjcc.v9.i12.2930DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058660PMC
April 2021

The Psychological Status of General Population in Hubei Province During the COVID-19 Outbreak: A Cross-Sectional Survey Study.

Front Public Health 2021 22;9:622762. Epub 2021 Apr 22.

Medical Imaging Center, First Affiliated Hospital of Jinan University, Guangzhou, China.

The current outbreak of the novel coronavirus disease 2019 (COVID-19), originating from Wuhan (Hubei, China), has rapidly spread across China and several other countries. During the outbreak of COVID-19, mental health of the general population in Hubei province may be affected. This study aimed to assess the psychological status and associated risk factors of the general population in Hubei province during the COVID-19 outbreak. A cross-sectional online survey was used to evaluate the symptoms of posttraumatic stress disorder (PTSD), depression, and anxiety, which were assessed by the Chinese version of the Impact of Event Scale-Revised, the Patient Health Questionnaire 9, and the seven-item Generalized Anxiety Disorder Scale, respectively. Coping style was assessed by the Simplified Coping Style Questionnaire. Multivariate logistic regression analysis was carried out to detect factors associated with mental health outcomes. Among 9,225 participants, 44.5% rated symptoms of PTSD, and 17.9 and 12.7% suffered from moderate and severe symptoms of depression and anxiety, respectively. Individuals who were geographically located in Wuhan and familiar with someone who has COVID-19 had more severe symptoms of PTSD, depression, and anxiety, as well as a higher score in passive coping style ( < 0.05). Multivariate logistic regression analysis showed that people who were geographically located in Wuhan [odds ratio (OR) = 1.25, 95% confidence interval (CI) = 1.14-1.36, < 0.001] were associated with severe symptoms of PTSD. Besides, individuals who were familiar with someone who had COVID-19 (OR = 2.33, 95% CI = 2.07-2.63, < 0.001; OR = 1.90, 95% CI = 1.66-2.17, < 0.001; OR = 2.06, 95% CI = 1.78-2.39, < 0.001) and had a higher score in passive coping style (OR = 1.16, 95% CI = 1.14-1.17, < 0.001; OR = 1.17, 95% CI = 1.15-1.19, < 0.001; OR = 1.17, 95% CI = 1.15-1.19, < 0.001) were associated with severe symptoms of PTSD, depression, and anxiety. Moreover, a higher score in active coping style (OR = 0.96, 95% CI = 0.95-0.97, < 0.001; OR = 0.94, 95% CI = 0.93-0.94, < 0.001; OR = 0.95, 95% CI = 0.94-0.96, < 0.001) was associated with a lower risk of symptoms of PTSD, depression, and anxiety. During the midphase of COVID-19 outbreak, quite a few people have mental health problems; nearly half of the respondents rated symptoms of PTSD, and approximately one-fifth reported moderate to severe symptoms of anxiety and depression. Our findings may lead to better comprehend the psychological status of the general public and alleviate the public mental health crisis during the COVID-19 outbreak.
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http://dx.doi.org/10.3389/fpubh.2021.622762DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100308PMC
May 2021

Differential proteomic analysis of tibial subchondral bone from male and female guinea pigs with spontaneous osteoarthritis.

Exp Ther Med 2021 Jun 15;21(6):633. Epub 2021 Apr 15.

Department of Spine Surgery, Beijing Jishuitan Hospital, Beijing 100035, P.R. China.

A proteomic study on the tibial subchondral bone in guinea pigs with spontaneous osteoarthritis was performed to investigate the molecular alterations that occur in early osteoarthritis. A total of 132 healthy Hartley guinea pigs (aged 1 month; 66 female and 66 male) were randomly divided into 11 groups of six. Changes in articular cartilage and tibial subchondral bone were assessed using macroscopic examinations and micro-computed tomography. iTRAQ-integrated liquid chromatography-tandem mass spectrometry was used to identify differentially altered proteins in the tibial subchondral bone between 1- and 3-month-old guinea pigs, which were then validated using western blotting. A gradual progression of cartilage degeneration was observed in the knee joints of the subject animals from 5-11 months. With aging, the tibial subchondral trabecular bone acquired more plate-like and less anisotropic properties, with increased bone mineral density, bone volume, trabecular thickness and numbers. The proteomic study identified 138 and 113 proteins significantly differentially expressed between 3- and 1-month old guinea pigs in both the male and female animals, respectively. Western blotting confirmed the increased expression of osteoblast-associated protein S100 calcium-binding protein A8 (S100A8) and the deregulated expression of osteoclast-associated proteins coronin 1A (CORO1A) and T-cell immune regulator 1 (TCIRG1) in the 3-month old guinea pigs in comparison to the 1-month old guinea pigs. Spontaneous cartilage degeneration in the knee joints of male Hartley guinea pigs tended to be more serious compared with the females during the development of osteoarthritis. Together, the results suggest that osteoblast-associated protein S100A8 and osteoclast-associated proteins CORO1A and TCIRG1 are potentially key regulators of early osteoarthritic development in tibial subchondral bone.
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http://dx.doi.org/10.3892/etm.2021.10065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097192PMC
June 2021

[Research Progress of the Effects of Vitamin D Supplementation on Glycemic Control and Adverse Maternal-neonatal Outcomes in Gestational Diabetes Mellitus Patients].

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2021 Apr;43(2):253-258

School of Public Health,Hangzhou Normal University Division of Health Sciences,Hangzhou 311121,China.

Gestational diabetes mellitus(GDM)can cause blood glucose disorders in pregnant women and result in adverse maternal-neonatal outcomes.Vitamin D(VD)can improve glucose tolerance and insulin sensitivity,and thus theoretically,VD supplementation during pregnancy could improve glycemic control as well as maternal-neonatal outcomes in GDM patients.Although studies have shown that VD deficiency is associated with poor maternal-neonatal outcomes in GDM patients,no solid conclusion has been drawn with regard to the effects of VD supplementation on these patients.Therefore,here we summarized the research progress of the effects of VD supplementation on glycemic control and adverse maternal-neonatal outcomes in GDM patients,in an effort to guide the clinical VD supplementation during pregnancy.
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http://dx.doi.org/10.3881/j.issn.1000-503X.13177DOI Listing
April 2021

Clinical characteristics of sinonasal inverted papilloma associated with recurrence and malignant transformation.

Authors:
Xindi He Ying Wang

Auris Nasus Larynx 2021 May 5. Epub 2021 May 5.

Department of Rhinology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address:

Objective: Sinonasal inverted papilloma is a benign tumor but has a potential for recurrence and malignant transformation. The aim of this article is to analyze the clinical characteristics of sinonasal inverted papilloma associated with recurrence and malignant transformation.

Material And Methods: A retrospective study was performed in all patients with sinonasal inverted papilloma diagnosed between in our hospital during May 2013 and May 2018.

Results: A total of 151 patients were enrolled in this study. The average age of these patients was 52.24 years, with a male-to-female ratio of 2.775:1, and the most frequent clinical symptom was nasal obstruction. The recurrence rate was 39.07% (59/151), the mean time of recurrence was 35.8 months and most recurrences occur within the first three years after surgery. There was no significant difference in recurrence rate between all four stages and between after endoscopic surgery and a combined endoscopic and external approach. The malignant transformation rate was 5.96% (9/151) and the mean time of malignant transformation was 9.06 months.

Conclusions: Because of its high recurrence rate and the potential of malignant transformation, so it is important to determine the primary site of the tumor and to make a complete removal and a follow-up of at least five years after surgery is recommended.
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http://dx.doi.org/10.1016/j.anl.2021.04.008DOI Listing
May 2021

Synergy coordination of cellulose-based dialdehyde and carboxyl with Fe recoverable conductive self-healing hydrogel for sensor.

Mater Sci Eng C Mater Biol Appl 2021 Jun 1;125:112094. Epub 2021 Apr 1.

State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China. Electronic address:

A novel dual ionic network cellulose-based composite conductive self-healing hydrogel was fabricated with high elongation, rapid recoverability, high conductive sensitivity, self-healing ability and good biocompatibility. The hydrogel was constructed by the synergistic complexations of new-fashioned bidentate aldehyde groups on dialdehyde cellulose nanofibers (DACNFs) and carboxyl groups of acrylic acid (AA) with Fe. The elongation (~1300%) of the hydrogel containing 1 wt% DACNFs was approximate 13-fold of the pure PAA hydrogel and can recover to original state within 2 min after 80% compression. The self-healing efficiency increased with the addition of DACNFs in the dual ionic network cellulose-based composite conductive self-healing hydrogel. The hydrogel configured for a wearable test and showed high stretching sensitivity with a gauge factor of 13.82 at strain within 1.6%. The gauge factor (GF) decrease with the incremental strain within 20%. GF were 0.696 between 20% and 300% strain, 0.837 within 300% and 500%. Meanwhile, the current had a good linear relationship with the bending angles of hydrogels and pressure on hydrogels, which may provide a great potential in monitor both minor variations and large movements.
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http://dx.doi.org/10.1016/j.msec.2021.112094DOI Listing
June 2021

The different interactions of two anticancer drugs with bovine serum albumin based on multi-spectrum method combined with molecular dynamics simulations.

Spectrochim Acta A Mol Biomol Spectrosc 2021 May 6;259:119809. Epub 2021 May 6.

College of Chemistry and Chemical Engineering, Qiqihar University, No. 42, Wenhua Street, Qiqihar, PR China.

Paclitaxel is the best natural anticancer drug and artemisinin also has anticancer effect. In this study, the interactions between BSA and these two drugs were determined in PBS (pH 7.40) by multi-spectroscopic method and molecular dynamics (MD) simulations. The results showed that paclitaxel and artemisinin could statically quench the BSA fluorescence when the complexes were formed and the stoichiometric ratio of BSA-drugs was 1:1. Particularly, the BSA-paclitaxel complex was more stable than BSA-artemisinin complex. During the binding, the surroundings around Trp residue site was largely affected than Tyr site, especially Trp 214 to a more hydrophobic environment. In addition, the binding processes were mainly spontaneous through electrostatic force interaction. In summary, we concluded that the free drug of paclitaxel in blood was low and duration time of artemisinin was shorter.
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http://dx.doi.org/10.1016/j.saa.2021.119809DOI Listing
May 2021

Polyethylene mesh knitted fabrics mulching the soil to mitigate China's haze: A potential source of PBDEs.

Chemosphere 2021 Apr 30;280:130689. Epub 2021 Apr 30.

Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, 300134, China.

The fate of polybrominated diphenyl ethers (PBDEs) from polyethylene mesh knitted fabrics (PMKFs) to mulched soil and nearby plants was studied. PBDEs in the soil sample collected from Tianjin University of Commerce in April 2019 increased significantly after 6 months of PMKF mulching owing to PMKFs as the main input source. The compositional profiles/congener patterns of the PBDEs in the soil and PMKFs became similar after 6 months. High correlations were found between ΣPBDEs in the soil and PMKFs in October 2019, with no significant correlation in April. Plants could take up, accumulate and biotransform PBDEs in contaminated soil. The uptake of BDE-209 by plants was the highest compared with other lesser brominated PBDE congeners, due to its higher log Kow value and molecular weight or size. BDE-47 taken up in the plant was biotransformed via hydroxylation. These results prove that the government's PMKF solution to haze is causing environmental problems in bare soil, i.e., PBDE pollution in both soil and nearby plants. The present study provides important pieces of evidence for government and policymakers, and it is recommended that one environmental problem is not solved by creating another.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130689DOI Listing
April 2021

Molecular mechanism of apelin-13 regulation of colonic motility in rats.

Eur J Pharmacol 2021 May 4:174149. Epub 2021 May 4.

Department of Gastroenterology, Renmin Hospital of Wuhan University, 430060, Wuhan, Hubei Province, China. Electronic address:

Apelin is a novel neuropeptide identified as the endogenous ligand for the apelin receptor. Apelin and its receptor are widely distributed in the gastrointestinal tract. Studies have reported that apelin-13 is involved in modulating gastrointestinal motility; however, the evidence is insufficient and the relevant mechanism is still not fully clear. Consequently, our study designed to explore the effect induced by exogenous apelin-13, to analyze the mechanism of action in isolated rat colons and colonic smooth muscle cells. The spontaneous contractions of colonic smooth muscle strips from rat were measured in an organ bath system. L-type calcium currents and large conductance Ca-activated K (BK) currents in rat colonic smooth muscle cells were investigated using the electrophysiological patch-clamp technique. Apelin-13 decreased the spontaneous contractile activity of colonic smooth muscle strips in a dose-dependent manner, and the inhibitory effect was not abolished by tetrodotoxin. The electrophysiological recordings revealed that apelin-13 reduced the crest currents of L-type calcium in a concentration-dependent manner in colonic smooth muscle cells at the test potential of 0 mV. Moreover, apelin-13 moved the current-voltage (I-V) curves of L-type calcium channels upward, but did not change their contour. Furthermore, the characteristics of L-type calcium channels with steady-state activation and steady-state inactivation were not significantly changed. Similarly, application of apelin-13 also significantly decreased BK currents in a concentration dependent manner. In conclusion, apelin-13 inhibited the spontaneous contractile activity of isolated rat colons via the suppression of L-type calcium channels and BK channels in colonic smooth muscle cells.
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http://dx.doi.org/10.1016/j.ejphar.2021.174149DOI Listing
May 2021