Publications by authors named "Ying Huang"

2,639 Publications

  • Page 1 of 1

Environmental exposure to perfluoroalkyl substances in early pregnancy, maternal glucose homeostasis and the risk of gestational diabetes: A prospective cohort study.

Environ Int 2021 May 10;156:106621. Epub 2021 May 10.

Ministry of Education -Shanghai Key Laboratory of Children's Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai Jiao Tong University School of Public Health, Shanghai, China. Electronic address:

Background: Humans are widely exposed to environmental perfluoroalkyl substances (PFAS), which may affect glucose homeostasis. However, research linking PFAS exposure to glucose homeostasis during pregnancy is limited and the results were inconsistent. We aimed to investigate the association between PFAS exposure and glucose homeostasis in pregnancy in a large prospective cohort.

Methods: A total of 2747 pregnant women who participated in the Shanghai Birth Cohort, had blood samples in early pregnancy and completed a 75 g oral glucose tolerance test (OGTT) at 24-28 gestational weeks were included. 10 PFAS were determined by high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS-MS) in the plasma samples in early pregnancy. Logistic regression was used to explore the associations between PFAS concentrations and gestational diabetes mellitus (GDM), while multiple linear regression was used to model the associations between PFAS and OGTT fasting, 1-h and 2-h glucose levels. Potential confounders were adjusted. Bayesian kernel machine regression (BKMR) and a quantile-based g-computation approach (qgcomp) were employed to explore the joint and independent effects of PFAS on glucose homeostasis.

Results: The incidence of GDM was 11.8%. One log-unit increment in plasma concentrations in early pregnancy was associated with an increased risk of GDM for perfluorobutane sulfonate (PFBS) (adjusted odd ratio (aOR) = 1.23, 95% confidence interval (95% CI): 1.05, 1.44) and perfluoroheptanoic acid (PFHpA) (aOR = 1.25, 95% CI: 1.07, 1.46). Perfluorooctane sulfonic acid (PFOS), perfluorononanoic acid (PFNA), perfluorodecanoic acid (PFDA), perfluorohexanesulfonate (PFHxS) and PFHpA were positively correlated with 1-h and 2-h glucose levels. Results of the mixed exposure model showed that the joint effects of PFAS were significantly associated with abnormal glucose homeostasis; In the BKMR model, PFAS mixture exposure was positively associated with the GDM incidence, 1-h and 2-h glucose levels and negatively correlated with FBG level. A similar trend could be observed in qgcomp and the positive correlation between PFAS and 2-h glucose level was significant (β = 0.12, 95% CI: 0.04, 0.20). PFOS, PFNA and PFHpA may be the main contributors after controlling for other PFAS congeners. PFOS was significantly correlated with GDM incidence and 2-h glucose level, and PFHpA was significantly associated with FBG and 2-h glucose levels. The above associations were more prominent among women with a normal prepregnant BMI.

Conclusions: Environmental exposure to PFAS may affect glucose homeostasis in pregnancy and increase the risk of GDM, especially in normal weight women.
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http://dx.doi.org/10.1016/j.envint.2021.106621DOI Listing
May 2021

In Situ, Atomic-Resolution Observation of Lithiation and Sodiation of WS Nanoflakes: Implications for Lithium-Ion and Sodium-Ion Batteries.

Small 2021 May 13:e2100637. Epub 2021 May 13.

Department of Materials Science and Engineering, Northwestern University, Evanston, IL, 60208, USA.

WS nanoflakes have great potential as electrode materials of lithium-ion batteries (LIBs) and sodium-ion batteries (SIBs) because of their unique 2D structure, which facilitates the reversible intercalation and extraction of alkali metal ions. However, a fundamental understanding of the electrochemical lithiation/sodiation dynamics of WS nanoflakes especially at the nanoscale level, remains elusive. Here, by combining battery electrochemical measurements, density functional theory calculations, and in situ transmission electron microscopy, the electrochemical-reaction kinetics and mechanism for both lithiation and sodiation of WS nanoflakes are investigated at the atomic scale. It is found that compared to LIBs, SIBs exhibit a higher reversible sodium (Na) storage capacity and superior cyclability. For sodiation, the volume change due to ion intercalation is smaller than that in lithiation. Also, sodiated WS maintains its layered structure after the intercalation process, and the reduced metal nanoparticles after conversion in sodiation are well-dispersed and aligned forming a pattern similar to the layered structure. Overall, this work shows a direct interconnection between the reaction dynamics of lithiated/sodiated WS nanoflakes and their electrochemical performance, which sheds light on the rational optimization and development of advanced WS -based electrodes.
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http://dx.doi.org/10.1002/smll.202100637DOI Listing
May 2021

Individualized Fraction Regimen of SBRT Patients With Non-Small Cell Lung Cancer Based on Uncomplicated and Cancer-Free Control Probability.

Technol Cancer Res Treat 2021 Jan-Dec;20:15330338211011967

Department of Radiation Oncology, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China.

Introduction: Stereotactic body radiotherapy (SBRT) currently adopts non-discriminative prescription regimen. This study attempts to investigate an individualized fraction regimen (IFR) method for SBRT patients with non-small cell lung cancer (NSCLC) based on Uncomplicated and Cancer-free Control Probability (UCFCP).

Methods: Twenty patients with NSCLC were retrospectively prescribed with 40 regimens, ranging from 8Gy×5f to 12Gy×5f in step of 0.1 Gy. Taking into consideration of the age and the BMI index of each patient as well, the tumor control probability (TCP), the normal tissue complication probability (NTCP) of the total lung, chest wall and rib, and the secondary cancer probability (SCP) of the total lung were calculated for each plan of the patients. For the 40 regimens, the UCFCP was calculated and the maximum value of UCFCP was the IFR of the specified patient. Besides, IFR of UCP approach which only took account of the TCP and NTCP was also derived and to be compared with the IFR based on the UCFCP method.

Results: For all the patients, the UCFCP value showed a bell-shaped trend with the change of prescription dose. Among the 20 patients, the IFRs of 16 patients were different from the original fixed regimen. Of the 16 patients, the IFR of 5 patients exhibited slight changes between UCP and UCFCP methods.

Conclusion: The method based on the maximum value of UCFCP function may be helpful to provide IFR for specific SBRT patients with NSCLC, differentiating the patient specific characteristics such as anatomical structures and locations.
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http://dx.doi.org/10.1177/15330338211011967DOI Listing
May 2021

A combined radical and non-radical oxidation processes for efficient degradation of Acid Orange 7 in the homogeneous Cu(II)/PMS system: important role of chloride.

Environ Sci Pollut Res Int 2021 May 12. Epub 2021 May 12.

School of Civil and Environmental Engineering, Ningbo University, Ningbo, 315211, People's Republic of China.

Trace copper ion (Cu(II)) in water and wastewater can trigger peroxymonosulfate (PMS) activation to oxidize organic compounds, but it only works under alkaline conditions. In this work, we found that the presence of chloride could significantly accelerate the oxidation of Acid Orange 7 (AO7) by the Cu(II)/PMS process at a wide pH range (4.0-9.0). The observed pseudo-first-order rate constant k for AO7 oxidation was linearly correlated with the increased Cl concentration (0-300 mM). An increase in mineralization rate was observed in the presence of Cl, while the overall mineralization was quite low. Decomposition of PMS facilitated when Cl concentration or pH value increased. Based on the scavenger experiments and electron paramagnetic resonance (EPR) measurement, the mechanism of Cu(II)-catalyzed PMS oxidation process in the presence of Cl was proposed as both the radical and non-radical pathway, and O was the reactive oxygen species in the Cu(II)/PMS system. Finally, a possible degradation pathway of AO7 was elucidated. The feasibility of in situ utilizing high salinity and trace cupric species to accelerate the degradation of organic pollutants by the Cu(II)/PMS process in water and wastewater was demonstrated. However, the identification of undesired chlorinated by-products reminds us of cautiousness in assessing the application of Cu(II)/PMS system under chloride-rich environment. The findings of this work provide a simple and efficient approach to apply PMS in the remediation of refractory organic contaminants in the presence of trace cupric species under a high salinity environment with a wide range of pH.
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http://dx.doi.org/10.1007/s11356-021-14262-1DOI Listing
May 2021

The Dual Function of KDM5C in Both Gene Transcriptional Activation and Repression Promotes Breast Cancer Cell Growth and Tumorigenesis.

Adv Sci (Weinh) 2021 05 18;8(9):2004635. Epub 2021 Feb 18.

State Key Laboratory of Cellular Stress Biology Fujian Provincial Key Laboratory of Innovative Drug Target Research School of Pharmaceutical Sciences Xiamen University Xiang'an South Road Xiamen Fujian 361102 China.

Emerging evidence suggested that epigenetic regulators can exhibit both activator and repressor activities in gene transcriptional regulation and disease development, such as cancer. However, how these dual activities are regulated and coordinated in specific cellular contexts remains elusive. Here, it is reported that KDM5C, a repressive histone demethylase, unexpectedly activates estrogen receptor alpha (ER)-target genes, and meanwhile suppresses type I interferons (IFNs) and IFN-stimulated genes (ISGs) to promote ER-positive breast cancer cell growth and tumorigenesis. KDM5C-interacting protein, ZMYND8, is found to be involved in both processes. Mechanistically, KDM5C binds to active enhancers and recruits the P-TEFb complex to activate ER-target genes, while inhibits TBK1 phosphorylation in the cytosol to repress type I IFNs and ISGs. Pharmacological inhibition of both ER and KDM5C is effective in inhibiting cell growth and tumorigenesis. Taken together, it is revealed that the dual activator and repressor nature of an epigenetic regulator together contributes to cancer development.
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http://dx.doi.org/10.1002/advs.202004635DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097366PMC
May 2021

Retro-orbital injection of FITC-dextran combined with isolectin B4 in assessing the retinal neovascularization defect.

BMC Ophthalmol 2021 May 11;21(1):208. Epub 2021 May 11.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Jinsui Road 7, Tianhe District, 510000, Guangzhou, China.

Background: A reliable and effective method is required to deliver agent that can aid the in vivo imaging of retinal vessels. The aim of the present study was to evaluate retro-orbital (RO) injection of fluorescein-labeled isothiocyanate dextran (FITC-dextran) as a method of demonstrating retinal neovascularization (NV) and avascular areas in oxygen-induced retinopathy (OIR) mice.

Methods: Different concentrations of FITC-dextran were used to compare the efficacy of this agent in perfusing the retinal vessels. Hematoxylin-eosin (HE) staining was used to evaluate the safety of RO injection. The vitreous blood vessels and extent of NV were assessed in P17 OIR mice using FITC-dextran and compared with the corresponding measurements obtained following isolectin B4 staining or the combination of both methods.

Results: The fluorescence of small vessels and neovascular tufts could be observed clearly following RO injection of 0.05 ml of 25 mg/ml or 50 mg/ml FITC-dextran. No visible damage to tissues adjacent to the injection site was discovered. Vitreous blood flow was gradually reduced from P0 to P5 and eventually disappeared in P17 OIR mice, as demonstrated by FITC-dextran perfusion. The retinal NV areas assessed by isolectin B4 were larger than those assessed by FITC-dextran, but the retinal avascular areas were smaller. The combination of both methods could conduce to distinguish non-functional blood vessels.

Conclusions: RO injection of FITC-dextran combined with isolectin B4 is an effective, optimal method for assessing the NV area and avascular area.
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http://dx.doi.org/10.1186/s12886-021-01969-5DOI Listing
May 2021

sp. nov., isolated from the respiratory tract of , and sp. nov., isolated from the lung tissue of .

Int J Syst Evol Microbiol 2021 May;71(5)

Shanghai Institute for Emerging and Re-emerging Infectious Diseases, Shanghai Public Health Clinical Center, Shanghai 201508, PR China.

Four Gram-stain-positive, non-motile and asporous bacilli (strains ZJ-599, ZJ-621, MC1420 and MC1482), isolated from animal tissue and environmental samples collected on the Qinghai-Tibet Plateau, PR China, were taxonomically characterized. Based on the results of 16S rRNA gene sequence analyses, the closest relatives of strains ZJ-599 and ZJ-621 were LMM-1653 (97.5 %), M408/89/1 (96.5 %) and OJ8 (96.3 %), whereas strains MC1420 and MC1482 were closest to CCUG 58655 (98.9 %), DSM 20632 (98.4 %) and DSM 44291 (97.9 %). The results of gene sequence similarity analysis indicated that M408/89/1 and CCUG 58655 were closest to strains ZJ-599/ZJ-621 (83.5 %) and MC1420/MC1482 (91.8 %), respectively. The two novel type strains shared a similarity of 95.2 % in 16S rRNA and 81.3 % in gene sequences. The TAP-PCR DNA fingerprint and MALDI-TOF MS spectrum patterns clearly differentiated the novel isolates within and between each pair of strains. Strain ZJ-599 had 21.9-22.4 % digital DNA-DNA hybridization (dDDH) scores with LMM-1653, M408/89/1 and OJ8, and 72.3-72.9 % of average nucleotide identity (ANI) with them. Similarly, strain MC1420 had 22.9-23.7 % dDDH values with CCUG 58655, DSM 20632 and DSM 44291, and 80.4-81.3 % ANI scores with them. Strain ZJ-599 had a 23.1 % dDDH value and 70.5 % ANI score with strain MC1420, both below the corresponding thresholds for species delineation. Strains ZJ-599 and MC1420 both contain mycolic acids and have MK-8(H) and MK-9(H) as the predominant respiratory quinones, -diaminopimelic acid as the diagnostic diamino acid, and C9 as the main fatty acid. C8 and C8 were predominant in strain ZJ-599 in contrast to C7 being predominant in strain MC1420. The main polar lipids in strain ZJ-599 were diphosphatidylglycerol, phosphatidylinositol and one unidentified glycolipid, while strain MC1420 had diphosphatidylglycerol, phosphatidylglycerol and one unidentified lipid as the major components. Since the two pairs of novel strains (ZJ-599/ZJ-621, MC1420/MC1482) distinctly differ from each other and from their nearest relatives, two novel species of the genus are proposed, namely (type strain ZJ-599=GDMCC 1.1779=JCM 34341) and (type strain MC1420=GDMCC 1.1783=JCM 34340), respectively.
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http://dx.doi.org/10.1099/ijsem.0.004803DOI Listing
May 2021

Increased blood alpha-carotene, all-trans-Beta-carotene and lycopene levels are associated with beneficial changes in heart rate variability: a CVD-stratified analysis in an adult population-based study.

Nutr J 2021 May 11;20(1):43. Epub 2021 May 11.

Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang University, No. 1 Minde Road, Donghu District, Nanchang, 330006, Jiangxi, China.

Background: Although the associations of antioxidant micronutrients, such as carotenoids and vitamins, with cardiovascular diseases (CVDs) have been studied extensively, blood concentrations of antioxidant micronutrients and heart rate variability (HRV), which has been proven to be an indicator of cardiac autonomic control, has not been reported. We aimed to explore whether blood concentrations of antioxidant micronutrients, including carotenoids and vitamins, are associated with elevated heart rate variability (HRV (beneficial change) in a cross-sectional analysis.

Methods: Data were obtained from the Midlife in the United States (MIDUS) study that includes a general adult population. A total of 1074 (aged 34-84) individuals were included. Multivariable analyses were performed to investigate the association between main blood carotenoids (total lutein, zeaxanthin, beta-cryptoxanthin, 13-cis-beta-carotene, alpha-carotene, all-trans-beta-carotene and total lycopene) and vitamins A (retinol) and E (gamma-tocopherol and alpha-tocopherol) and HRV after adjustments were made for lifestyle factors and age-related confounders.

Results: Pearson correlation analyses showed that the increased levels of carotenoids and vitamins were positively correlated with higher HRV (all P < 0.05). After adjustments were made for age, gender, race, body mass index(BMI), ever-smoker, number of drinking years and exercise, blood alpha-carotene, all-trans-beta-carotene and total lycopene levels were independently associated with higher HRV in the linear regression model (all P < 0.05). Sensitivity analysis by adding "ever chronic respiratory diseases" as a covariate suggested that blood concentrations of these three carotenoids were still associated with higher low-frequency (LF)-HRV and high-frequency (HF)-HRV (all P < 0.05). Furthermore, stratified analyses suggested that the associations were affected by adding "heart disease" and "hypertension" as covariates.

Conclusions: We provide the first evidence that elevated blood concentrations of alpha-carotene, trans-beta-carotene and lycopene are associated with beneficial changes in HRV in the general population. Daily intake of fruit and vegetables may be beneficial to increase blood carotenoid status and further prevent autonomic dysfunction.
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http://dx.doi.org/10.1186/s12937-021-00700-wDOI Listing
May 2021

Horizontal gene transfer of copper-containing membrane-bound monooxygenase (CuMMO) and di-iron soluble monooxygenase (SDIMO) in ethane- and propane-oxidizing .

Appl Environ Microbiol 2021 May 7. Epub 2021 May 7.

Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, School of Life Sciences, Fudan University, Shanghai, China

The families of copper-containing membrane-bound monooxygenases (CuMMOs) and soluble di-iron monooxygenases (SDIMOs) are not only involved in methane oxidation but also in short-chain alkane oxidation. Herein, we describe sp. ZPP, a bacterium able to grow with ethane or propane as the sole carbon and energy source and report on horizontal gene transfer (HGT) of actinobacterial hydrocarbon monooxygenases (HMO) of the CuMMO family and sMMO (soluble methane monooxygenase)-like SDIMO in the genus The key function of HMO in strain ZPP for propane oxidation was verified by allylthiourea inhibition. The HMO genes (designated ) and those encoding sMMO-like SDIMO (designated ) are located on a linear mega-plasmid (pRZP1) of strain ZPP. Comparative genomic analysis of similar plasmids indicated mobility of these plasmids within the genus The plasmid pRZP1 in strain ZPP could be conjugatively transferred to a recipient in a mating experiment and showed similar ethane and propane consuming activities. Finally, our findings demonstrate that horizontal transfer of plasmid-based CuMMO and SDIMO genes confers the ability to use ethane and propane on the recipient.CuMMOs and SDIMOs initiate the aerobic oxidation of alkanes in bacteria. Here, the supposition that horizontally transferred plasmid-based CuMMO and SDIMO genes confer on the recipient the similar ability to use ethane and propane was proposed and confirmed in This study is a living example of HGT of CuMMOs and SDIMOs and outlines the plasmid-borne properties responsible for gaseous alkane-degradation. Our results indicate that plasmids can support rapid evolution of enzyme-mediated biogeochemical processes.
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http://dx.doi.org/10.1128/AEM.00227-21DOI Listing
May 2021

, a dwarf new species of Amaryllidaceae from Hunan, China.

PhytoKeys 2021 21;177:1-9. Epub 2021 Apr 21.

College of Life Sciences, Anhui Normal University, Wuhu, Anhui 241000, China.

S.Y. Zhang, a new species from Hunan Province (central South China), is described and illustrated. This new species is a fertile diploid plant and its karyotype is 2n = 22. It is most similar to L. × haywardii in morphology, but the latter is a hybrid species and distributed in East China and the plant is much larger. Amongst the original species, is similar to , but differs from it in its flowers being rose-red (vs. red) and stamens and tepals are nearly the same length (vs. stamens significantly longer than tepals).
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http://dx.doi.org/10.3897/phytokeys.177.62741DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081704PMC
April 2021

Two-photon induced polymerization in a porous polymer film to create multi-layer structures.

Chem Commun (Camb) 2021 May;57(37):4516-4519

State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, P. R. China.

Two-photon induced polymerization for three-dimensional (3D) printing has attracted increasing attention. Here, we report the two-photon induced polymerization of triethylene glycol divinyl ether (TEGDVE) in a porous polymer film using 4,4',4''-nitrilotribenzoic acid (NTB) as the photosensitizer and diphenyliodonium hexafluorophosphate (HIP) as the initiator, enabling the printing of multi-layer structures in the porous support.
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http://dx.doi.org/10.1039/d1cc01383aDOI Listing
May 2021

Relationship between ABO blood groups and gestational hypertensive disorders: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 May;100(18):e25573

People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, Xinjiang Uygur Autonomous Region Province, China.

Background: The distribution of ABO blood group is related to the incidence of various diseases. Gestational hypertensive disorders (GHD) is one of the most important risk factors during pregnancy, which has certain heredity. It is reported that ABO blood type is associated with the risk of GHD. However, the results are still controversial. In this study, we conducted a systematic review and meta-analysis to clarify the relationship between ABO blood group and GHD.

Methods: All eligible studies come from Embase, Cochrane Library, Pubmed, Chinese databases SinoMed, Chinese National Knowledge Infrastructure, Chinese Scientific Journals Database, and Wanfang Data. The retrieval time is from the establishment of the database to March 2021. The language will be limited to Chinese and English. The 2 reviewers will be responsible for the selection of the study, the extraction of data, and the evaluation of the quality of the research. Pooled odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the corresponding associations. Sensitivity analysis, publication bias assessment, and heterogeneity test were performed using STATA 16.0.

Results: The results of this meta-analysis will be published in peer-reviewed journals.

Conclusion: This study will provide evidence to support the relationship between ABO blood group and the risk of GHD.

Ethics And Dissemination: The private information from individuals will not be published. This systematic review also will not impair endangering participants' rights. Ethical approval is not required. The results may be published in a peer-reviewed journal or disseminated in relevant conferences.

Osf Registration Number: DOI 10.17605/OSF.IO/3X9YZ.
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http://dx.doi.org/10.1097/MD.0000000000025573DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104180PMC
May 2021

The 100 top-cited articles in diabetic kidney disease: a bibliometric analysis.

Ren Fail 2021 Dec;43(1):781-795

Department of Nephrology, The Second Xiangya Hospital, Central South University, Changsha, China.

Background: Tremendous scientific researches have been conducted in the field of diabetic kidney disease (DKD), while few bibliometric analyses have been performed. We aim to identify 100 top-cited published articles about DKD and analyze their main characteristics quantitatively.

Methods: Web of Science was searched with the term 'diabetic kidney disease' OR 'diabetic nephropathy' to identify the top 100 most cited articles. For articles meeting the predefined criteria, the following data were extracted and analyzed: citation ranking, publication year, publication journal, journal impact factor, country and institution, authors, study type, and keywords.

Results: The highest number of citations was 4753 times. The median average citations per year was 21.8 (IQR, 16.6-33.0). Most articles focused on the pathogenesis and treatment. These articles were published in 25 different journals and the published the greatest number (20%). Forty-three articles (43%) originated from the United States. The University of Groningen was the leading institute, contributing five top-cited articles. The most frequent first author was de Zeeuw ( = 4), followed by Parving ( = 3). There was no correlation between the average citations and the number of authors, the number of institutes, or the number of funds, respectively. Experimental animal study was the research type most frequently conducted ( = 30), followed by observational study ( = 24). Keyword analysis revealed transforming growth factor-β, oxidative stress, proteinuria, and renin-angiotensin-aldosterone system interruption are classic research topics. Sodium-glucose cotransporter 2 inhibitors, glucagon-like peptide 1 receptor agonists, and anti-inflammatory agents are the emerging trends of DKD.

Conclusions: This bibliometric analysis helps in identifying the milestones, inadequacies, classic hotspots, and emerging trends of DKD. Pathogenesis and treatment are core themes in DKD research, while high-quality articles on the prediction and biomarker are insufficient. New analyzing metrics are needed to assess the actual impact of these top-cited articles on clinical practice.
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http://dx.doi.org/10.1080/0886022X.2021.1919528DOI Listing
December 2021

Establishment of an anti-inflammation-based bioassay for the quality control of the 13-component TCM formula (Lianhua Qingwen).

Pharm Biol 2021 Dec;59(1):537-545

Department of Hematology, The Fifth Medical Center of Chinese, PLA General Hospital, Beijing, China.

Context: Owing to the complexity of chemical ingredients in traditional Chinese medicine (TCM), it is difficult to maintain quality and efficacy by relying only on chemical markers.

Objective: Lianhua Qingwen capsule (LHQW) was selected as an example to discuss the feasibility of a bioassay for quality control.

Materials And Methods: Network pharmacology was used to screen potential targets in LHQW with respect to its anti-inflammatory effects. An cell model was used to validate the prediction. An anti-inflammatory bioassay was established for the quality evaluation of LHQW in 40 batches of marketed products and three batches of destructed samples.

Results: The tumor necrosis factor/interleukin-6 (TNF/IL-6) pathway via macrophage was selected as the potential target of LHQW. The IC value of LHQW on RAW 264.7 was 799.8 μg/mL. LHQW had significant inhibitory effects on the expression of IL-6 in a dose-dependent manner ( < 0.05). The anti-inflammatory biopotency of LHQW was calculated based on the inhibitory bioactivity on IL-6. The biopotency of 40 marketed samples ranged from 404 U/μg to 2171 U/μg, with a coefficient of variation (CV) of 37.91%. By contrast, the contents of forsythin indicated lower CV (28.05%) than the value of biopotency. Moreover, the biopotencies of destructed samples declined approximate 50%, while the contents of forsythin did not change. This newly established bioassay revealed a better ability to discriminate the quality variations of LHQW as compared to the routine chemical determination.

Conclusions: A well-established bioassay may have promising ability to reveal the variance in quality of TCM.
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http://dx.doi.org/10.1080/13880209.2021.1917627DOI Listing
December 2021

One-pot synthesis of AuPd@FeO nanoagent with the activable Fe species for enhanced Chemodynamic-photothermal synergetic therapy.

Biomaterials 2021 Apr 27;274:120821. Epub 2021 Apr 27.

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China; School of Applied Chemical Engineering, University of Science and Technology of China, Road Baohe District, Hefei, Anhui, 230026, PR China. Electronic address:

Facile fabrication of Fe-based nanotheranostic agents with the enhanced Chemodynamic therapy (CDT) effect and multiple functions is important for oncotherapy. In this report, noble-metal@FeO core-shell nanoparticles (Au@FeO NPs, AuRu@FeO NPs, AuPt@FeO NPs and AuPd@FeO NPs) are one-pot constructed by a simply redox self-assembly strategy. As a typical example, AuPd@FeO NPs are applied for oncotherapy. Compared to their crystalline counterparts (e.g., AuPd@c-FeO nanocrystals (NCs)), AuPd@FeO NPs with the metastable FeO shell can be activated by a small amount of NaBH to obviously enhance the production of ·OH in subsequent Fenton reaction (these activated products are termed as r-AuPd@FeO NPs). In addition, a favorable photothermal effect (63.5% photothermal conversion efficiency) of r-AuPd@FeO NPs can further promote the ·OH generation. Moreover, r-AuPd@FeO NPs also show a pH-responsive T-weighted MRI contrast property, CT imaging capacity and the function of regulating tumor microenvironment. This work presents an attractive route to prepare versatile nanotheranostic agents.
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http://dx.doi.org/10.1016/j.biomaterials.2021.120821DOI Listing
April 2021

Study on the Bioassay of Anti-Inflammatory Effects of Fuke Qianjin Capsule Based on COX-2 Inhibiting Activity.

Evid Based Complement Alternat Med 2021 8;2021:6620124. Epub 2021 Apr 8.

Department of Poisoning Treatment, Fifth Medical Center of Chinese PLA General Hospital, Beijing 100071, China.

Fuke Qianjin Capsule (FKQJ) is a common TCM compound formula in the treatment of gynecological inflammation-related diseases. This study intends to explore and establish a bioassay method to further improve its quality control. The bioassay method for the determination of anti-inflammatory biopotency was established based on its inhibitory activity on recombinant human cyclooxygenase-2 (COX-2), an active target of FKQJ in the treatment of female pelvic inflammatory disease. We firstly established chemical fingerprint of 20 batches of FKQJ by ultra-high-performance liquid chromatography to identify the components and analyze the chemical similarities. The similarity within different batches of FKQJ was relatively high. The values of similarity of the 19 batches were between 0.973 and 0.995, while one batch's similarity value was 0.813. Celecoxib, a selective inhibitor of COX-2, was chosen as the positive control drug in COX-2 activity assay to establish an anti-inflammatory biopotency detection method based on parallel line test of qualitative response. The methodological investigation showed that the method possessed good repeatability and precision. Secondly, the anti-inflammatory biopotency of 20 batches of FKQJ for inhibiting COX-2 was determined. The results showed that the biopotency of different batches of FKQJ ranged from 676 U/g to 1310 U/g, with average value of 918 U/g and RSD of 16.7%. Based on multiple linear regression analysis, we found that three contents were highly correlated with the anti-inflammatory biopotency, while chlorogenic acid was validated of the strongest anti-inflammatory activity in vitro. Compared with chemical detection, bioassay can better reflect the quality fluctuation of different batches of products and correlate the known pharmacodynamic targets. The supplement of the bioassay method based on chemical evaluation is helpful to improve the quality control ability of Chinese patent medicine and ensure its clinical efficacy is stable and controllable.
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http://dx.doi.org/10.1155/2021/6620124DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049786PMC
April 2021

Transcriptome Analysis Reveals Possible Immunomodulatory Activity Mechanism of sp. Exopolysaccharides on RAW264.7 Macrophages.

Mar Drugs 2021 Apr 14;19(4). Epub 2021 Apr 14.

Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine and Biotechnology, GuangXi University for Nationalities, Nanning 530006, China.

In this study, the exopolysaccharides of sp. (CEP) were isolated to obtain the purified fraction CEP4. Characterization results showed that CEP4 was a sulfated heteropolysaccharide. The main monosaccharide components of CEP4 are glucosamine hydrochloride (40.8%) and glucuronic acid (21.0%). The impact of CEP4 on the immune activity of RAW264.7 macrophage cytokines was detected, and the results showed that CEP4 induced the production of nitric oxide (NO), TNF-α, and IL-6 in a dose-dependent pattern within a range of 6 μg/mL. A total of 4824 differentially expressed genes (DEGs) were obtained from the results of RNA-seq. Gene enrichment analysis showed that immune-related genes such as , , and were significantly upregulated, while the genes and were significantly downregulated. KEGG pathway enrichment analysis showed that DEGs were significantly enriched in immune-related biological processes, including toll-like receptor (TLR) signaling pathway, cytosolic DNA-sensing pathway, and C-type lectin receptor signaling pathway. Protein-protein interaction (PPI) network analysis showed that , , , , , , , , , and were the hub genes with an essential role in the immune activity of CEP4. The preliminary results of the present study revealed the potential mechanism of CEP4 in the immune regulation of RAW264.7 macrophages, suggesting that CEP4 is a promising immunoregulatory agent.
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http://dx.doi.org/10.3390/md19040217DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070752PMC
April 2021

Efficacy and safety of renal denervation for hypertension in patients with chronic kidney disease: a meta-analysis.

Int J Hyperthermia 2021 ;38(1):732-742

Interdisciplinary Center of Sleep Medicine, Charite-University Berlin, Berlin, Germany.

Background: Renal denervation (RDN) is a new treatment for hypertension in patients with chronic kidney disease (CKD), but its efficacy is still debated. This meta-analysis aimed to evaluate the efficacy and safety of RDN for hypertension in patients with CKD.

Methods: PubMed, Web of Science, EMBASE, and Ovid databases were searched for relevant studies published. We performed both fixed- and random-effects meta-analyses of the changes in blood pressure, estimated glomerular filtration rate (eGFR), and urinary albumin-to-creatinine ratio (UACR) after RDN.

Results: The meta-analysis included 238 patients from 11 single-center, non-randomized, uncontrolled studies. Office blood pressure and 24-hour ambulatory blood pressure (24 h-ABP) showed a significant reduction 1 month after RDN ( < 0.05). This decrease of 24 h-ABP persisted for 24 months after RDN showed difference systolic blood pressure ( < 0.001) and diastolic blood pressure ( = 0.001). The 24 h-ABP exhibited a similar trend in the subgroup analysis. eGFR measurements obtained at each time point of analysis after RDN were not significantly different from those obtained before ( > 0.05). UACR levels were significantly reduced at 3 months and 6 months after RDN ( < 0.001). After RDN, the heart rate showed no significant changes ( > 0.05), and few major complications were encountered.

Conclusions: The meta-analysis showed that RDN may be effective and safe for treating CKD patients with hypertension. Well-designed randomized controlled trials of RDN are urgently needed to confirm the safety and reproducibility of RDN and to assess its impact on clinical outcomes.
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http://dx.doi.org/10.1080/02656736.2021.1916100DOI Listing
January 2021

Degradation and chlorination mechanism of fumaric acid based on SO: an experimental and theoretical study.

Environ Sci Pollut Res Int 2021 Apr 28. Epub 2021 Apr 28.

School of Civil and Environmental Engineering, Ningbo University, Ningbo, 315211, People's Republic of China.

It is well known that chloride ions could affect the oxidation kinetics and mechanism of contaminant based on SO in the wastewater. Here, the degradation of an organic acid, fumaric acid (FA), was investigated in the presence of chloride (0-300 mM) by the Fe(II)/peroxymonosulfate (Fe(II)/PMS) system. A negative impact of chloride was observed on the rates of FA degradation. The degree of inhibitory effect was higher in Fe(II)/PMS addition order. Some chlorinated byproducts were identified during the FA oxidation process in the presence of Cl by the ultraperformance liquid chromatography and quadrupole-time of flight mass spectrometer (UPLC-QTOF-MS). With the increasing content of Cl, an accumulation of adsorbable organic halogen (AOX), an increase in acute toxicity, and an inhibition of mineralization were observed. According to the results of kinetic modeling, the production and transformation of oxidative species were dependent on Cl dosage and reaction time. SO was supposed to be the main radical for FA degradation with Cl concentration below 5 mM, whereas Cl was primarily responsible for the depletion of FA at [Cl] > 5 mM. A possible degradation pathway of FA was discussed. This study reveals the potential environmental risk of organic acid and is necessary to explore useful strategies for ameliorating the treatment of chloride-rich wastewater.
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http://dx.doi.org/10.1007/s11356-021-12756-6DOI Listing
April 2021

Role of Chronic Inflammatory Ratios in Predicting Recurrence of Resected Patients with Stage I-III Mucinous Colorectal Adenocarcinoma.

Cancer Manag Res 2021 20;13:3455-3464. Epub 2021 Apr 20.

School of Public Health; Jiangxi Provincial Key Laboratory of Preventive Medicine, Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.

Background: Cancer-related inflammation is the main cause of the progression of mucinous colorectal adenocarcinoma (MCA). Circulating fibrinogen-to-pre-albumin ratio (FPR) is associated with the clinical outcome in colorectal cancer (CRC). However, the prognostic role of FPR and which is the best inflammatory prognostic biomarker within MCA remain unknown.

Methods: We enrolled 157 patients with stage I-III MCA in this study. Kaplan-Meier curve, Cox regression, and time-dependent receiver operation characteristic curve analysis were performed to assess the prognostic value and efficacy of the neutrophil-to-albumin ratio (NAR), neutrophil-to-pre-albumin ratio (NPAR), albumin-to-alkaline phosphatase ratio (AAPR), albumin-to-globulin ratio (AGR), albumin-to-fibrinogen ratio (AFR), and FPR in these patients.

Results: We found that NAR, NPAR, and FPR were significantly associated with unsatisfactory recurrence-free survival (RFS) in patients with stage I-III MCA, and the predicted efficacy of FPR was superior to that of the other two inflammatory biomarkers. Moreover, patients with a high combined TNM-CA199-FPR score had worse outcomes, with a high predicted efficacy of up to 0.779 (0.703-0.856). Using FPR, the patient was monitored for the recurrence up to two months earlier than that achieved using the common imaging techniques (4 vs 6 median months) in stage I-III MCA patients undergoing radical resection.

Conclusion: FPR is the preferred inflammatory biomarker and commonly used for predicting and monitoring recurrence in stage I-III MCA patients. The combined TNM-CA199-FPR score is an economical, simple, effective, and independent prognostic factor for localized disease.
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http://dx.doi.org/10.2147/CMAR.S303758DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068493PMC
April 2021

Prognostic significance of carcinoembryonic antigen combined with carbohydrate antigen 19-9 following neoadjuvant chemoradiotherapy in patients with locally advanced rectal cancer.

Colorectal Dis 2021 Apr 26. Epub 2021 Apr 26.

Department of Colorectal Surgery, Fujian Medical University Union Hospital, Fuzhou, China.

Aim: The clinical significance of carcinoembryonic antigen (CEA) combined with carbohydrate antigen 19-9 (CA19-9) in patients with rectal cancer is not well established. The aim of this study was to determine the prognostic value of these combined tumour markers in patients with locally advanced rectal cancer (LARC) treated with neoadjuvant chemoradiotherapy (nCRT).

Methods: A total of 687 consecutive patients with LARC who underwent nCRT and radical surgery were analyzed. Tumour characteristics, recurrence-free survival (RFS) and overall survival (OS) were compared according to the number of elevated tumour markers measured before and after nCRT. In addition, the prognostic significance of perioperative changes in the combined tumour markers was further evaluated.

Result: The RFS and OS rates decreased in a stepwise manner in association with the number of elevated pre- and post-nCRT tumour markers (all P<0.05). Multivariate analysis showed that only the number of elevated post-nCRT tumour markers was an independent prognostic factor (both P<0.05). For 311 patients with elevated pre-nCRT tumour markers, normalization of the tumour markers after nCRT was an independent prognostic protective factor (both P<0.05), and patients with both markers elevated post-nCRT had a 2.5-fold and 3.7-fold increased risk of recurrence and death, respectively (P<0.05). Furthermore, normalization of post-nCRT tumour markers after surgery was also closely related to improved prognosis.

Conclusion: This combination of post-nCRT tumour markers can accurately predict the long-term survival of patients with LARC treated with nCRT and curative resection, and the normalization of the combined tumour markers after either nCRT or surgery was associated with better survival.
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http://dx.doi.org/10.1111/codi.15694DOI Listing
April 2021

Long-term changes in the gut microbiota after triple therapy, sequential therapy, bismuth quadruple therapy and concomitant therapy for Helicobacter pylori eradication in Chinese children.

Helicobacter 2021 Apr 25:e12809. Epub 2021 Apr 25.

Department of Gastroenterology, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, China.

Background: We previously reported that the administration of 14-day standard triple therapy (TT), sequential therapy (ST), bismuth-based quadruple therapy (BT), and concomitant therapy (CT) as the first-line therapy for Helicobacter pylori infection in Chinese children achieved eradication rates of 74.1%, 69.5%, 89.8%, and 84.6%, respectively. In this follow-up study, we further evaluated the short- and long-term effects of the four regimens on the gut microbiota in these children.

Methods: We prospectively recruited treatment-naïve children with H. pylori infection. Fecal samples were collected at week 0, 2, 6, and 52, and alterations in the gut microbiota were analyzed by 16S rRNA gene sequencing.

Results: Sixty-three patients participated in this study (16 with TT, 15 with ST, 16 with BT and 16 with CT). At week 2, the alpha diversity (Shannon and Chao 1 index) was significantly reduced in the TT (p = 0.008, p < 0.001), ST (p < 0.001, p < 0.001), BT (p < 0.001, p < 0.001) and CT groups (p < 0.001, p < 0.001). Some changes persisted in the ST, BT, and CT groups at week 6, and all were restored (expect p = 0.02 with Chao 1 index in the CT group) at week 52. The beta diversity was significantly changed in the BT (p = 0.001) and CT groups (p = 0.001) 2 weeks post-eradication and restored 1 year after therapy. Immediately after therapy, the relative abundance of Proteobacteria was strikingly increased in the ST (p = 0.005), BT (p < 0.001) and CT groups (p < 0.001), and the genus-level analysis showed that the abundances of 23.1%, 43.3%, 78.6%, and 78% of the bacterial genera in the TT, ST, BT, and CT groups were significantly changed. All these changes returned to almost the pre-eradication level 1 year post-eradication.

Conclusion: Eradication of H. pylori infection can lead to transient dysbiosis of gut microbiota, and these changes almost recovered 1 year post-eradication, which indicates the long-term safety of H. pylori therapy.
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http://dx.doi.org/10.1111/hel.12809DOI Listing
April 2021

Alternating current enhanced bioremediation of petroleum hydrocarbon-contaminated soils.

Environ Sci Pollut Res Int 2021 Apr 24. Epub 2021 Apr 24.

College of Chemical and Biological Engineering, Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Zhejiang University, Hangzhou, 310027, China.

In this work, bioremediation was applied with sinusoidal alternating current (AC) electric fields to remove petroleum hydrocarbon (TPH) for soil remediation. Applying AC electric field with bioremediation (AC+BIO) could efficiently remove 31.6% of the TPH in 21 days, much faster than that in the BIO only system (13.7%) and AC only system (5.5%). When the operation time extended to 119 days, the AC+BIO system could remove 73.3% of the TPH. Applying AC electric field (20-200 V/m) could maintain the soil pH at neutral, superior to the direct current electric field. The maximum difference between soil temperature and the room temperature was 1.9 °C in the AC (50 V/m) +BIO system. The effects of AC voltage gradient (20-200 V/m) on the microorganisms and TPH degradation efficiency by AC+BIO were investigated, and the optimized AC voltage gradient was assessed as 50 V/m for lab-scale experiments. The microbial community structures in the BIO and AC+BIO systems were compared. Although Pseudomonas was the dominant species, Firmicutes became more abundant in the AC+BIO system than the BIO system, indicating their adaptive capacity to the stress of the AC electric field. Real petroleum-contaminated soil was used as a reaction matrix to evaluate the performance of AC+BIO in the field. The initial current density was about 0.2 mA/cm, voltage gradient was about 20 V/m, and the average TPH degradation rate was 8.1 μg/g per day. This study provided insights and fundamental supports for the applications of AC+BIO to treat petroleum-polluted soils.
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http://dx.doi.org/10.1007/s11356-021-13942-2DOI Listing
April 2021

IRS4 promotes the progression of non-small cell lung cancer and confers resistance to EGFR-TKI through the activation of PI3K/Akt and Ras-MAPK pathways.

Exp Cell Res 2021 Apr 21;403(2):112615. Epub 2021 Apr 21.

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China. Electronic address:

IRS4 is a member of the insulin receptor substrate (IRS) protein family. It acts as a cytoplasmic adaptor protein, integrating and transmitting signals from receptor protein tyrosine kinases to the intracellular environment. IRS4 can induce mammary tumorigenesis and is usually overexpressed in non-small cell lung cancer (NSCLC). However, little is known about the role of IRS4 in the development and progression of lung cancer. In this study, we show that IRS4 knockout suppresses the proliferation, colony formation, migration, and invasion of A549 lung cancer cells, as well as tumor growth in a nude mouse xenograft model. In contrast, stable expression of IRS4 showed the opposite effects. As expected, IRS4 was found to activate the PI3K/Akt and Ras-MAPK pathways, and we also showed that IRS4 depletion significantly enhanced the sensitivity of EGFR tyrosine kinase inhibitor (EGFR-TKI)-resistant cells to gefitinib. Taken together, these results show that IRS4 promotes NSCLC progression and may represent a potential therapeutic target for EGFR-TKI-resistant NSCLC.
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http://dx.doi.org/10.1016/j.yexcr.2021.112615DOI Listing
April 2021

Plasma MicroRNA Biomarkers in Limited Volume Samples for Detection of Early Stage Pancreatic Cancer.

Cancer Prev Res (Phila) 2021 Apr 23. Epub 2021 Apr 23.

Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center

Early detection of pancreatic ductal adenocarcinoma (PDAC) is key to improving patient outcomes; however, PDAC is usually diagnosed late. Therefore, blood-based minimally invasive biomarker assays for limited volume clinical samples are urgently needed. A novel microRNA profiling platform (Abcam Fireplex-Oncology Panel) was used to investigate the feasibility of developing early detection miRNA biomarkers with 20ul plasma from a training set (58 stage II PDAC cases and 30 controls) and two validation sets (34 stage II PDAC cases and 25 controls; 44 stage II PDAC cases and 18 controls). miR-34a-5p (AUC = 0.77, 95% CI 0.66 to 0.87), miR-130a-3p (AUC = 0.74, 95% CI 0.63 to 0.84,), and miR-222-3p (AUC = 0.70, 95% CI 0.58 to 0.81,) were identified as significantly differentially abundant in plasma from stage II PDAC vs. controls. Although none of the miRNAs individually outperformed the currently used serological biomarker for PDAC, CA19-9, combining the miRNAs with CA 19-9 improved AUCs from 0.89 (95% CI 0.81 to 0.95) for CA 19-9 alone to 0.92 (95% CI 0.86 to 0.97), 0.94 (95% CI 0.89 to 0.98), and 0.92 (95% CI 0.87 to 0.97), respectively. Gene Set Enrichment Analyses of transcripts correlated with high and low expression of the three miRNAs in the TCGA PDAC sample set. These miRNA biomarkers, assayed in limited volume plasma together with CA19-9, discriminate stage II PDAC from controls with good sensitivity and specificity. Unbiased profiling of larger cohorts should help develop an informative early detection biomarker assay for diagnostic settings.
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http://dx.doi.org/10.1158/1940-6207.CAPR-20-0303DOI Listing
April 2021

Conversation Skills in Chinese-Speaking Preschoolers with Autism: The Contributing Role of Parents' Verbal Responsiveness.

J Autism Dev Disord 2021 Apr 23. Epub 2021 Apr 23.

Department of Educational Psychology, The Chinese University of Hong Kong, Hong Kong, China.

Children with autism spectrum disorder (ASD) have conversation deficits, yet the growth of conversation abilities is understudied, especially in Chinese-speaking populations. Little is known about whether their parents' verbal responsiveness and redirectives are related to their conversation skills. Children with ASD (N = 37; M = 5;5) and their parents contributed their language samples. These children interacted with their parents at four time points over nine months. The number of conversational turns and the proportion of child-initiated conversation (but not the proportion of children's appropriate responses) grew over nine months. After controlling for time, autism severity, and language skills, parents' verbal responsiveness positively predicted children's appropriate responses. Parents' redirectives negatively predicted the proportion of children's appropriate responses and the number of conversational turns.
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http://dx.doi.org/10.1007/s10803-021-05017-5DOI Listing
April 2021

Effects of imperatorin on apoptosis and synaptic plasticity in vascular dementia rats.

Sci Rep 2021 Apr 21;11(1):8590. Epub 2021 Apr 21.

School of Basic Medicine, Gannan Medical University, Ganzhou, 341000, Jiangxi, China.

In view of the complicated pathophysiological process of vascular dementia (VD), drugs for the clinical treatment of VD mainly target related risk factors, while drugs with excellent efficacy in cognitive function are still relatively lacking. Imperatorin (IMP), an active constituent extracted from angelica dahuricae and notopterygium Notopterygii, which has anti-inflammatory, vasodilator, anticoagulant, block calcium channel, anticonvulsant, and anti oxygen free radical injury properties. Therefore,the present study examined its effects on VD rats and the underlying molecular mechanisms, in order to provide promising therapeutic methods. VD was established by modified ligation of perpetual two-vessel occlusion (2VO). After 2VO surgery, IMP (2.5, 5, and 10 mg/kg) was administered by intraperitoneal injection for 12 consecutive weeks to evaluate therapeutic effects. Cognitive function was verified by the Morris water maze. The neuronal morphological changes were examined via Hematoxylin-Eosin staining. Real-Time PCR and Western blot were used for detecting pro- and antiapoptotic biomarkers, and the hippocampus synaptic damage was examined by Transmission electron microscope. We revealed that 2VO-induced cognitive impairment, hippocampus CA1 neuron damage, apoptosis and synaptic damage. IMP-treatment significantly improved 2VO-induced cognitive deficits and hippocampus neuron damage. Molecular analysis revealed that IMP inhibited apoptosis through the down regulation of Bax, Caspase-3 and upregulation of Bcl-2. Meanwhile, IMP-treatment markedly improved synaptic ultrastructure morphology, increased the SAZ length, PSD thickness and up-regulated PSD-95 expression. Collectively, our findings demonstrated that IMP was effective in the treatment of 2VO-induced VD via inhibiting apoptosis of hippocampus neurons and reducing the synaptic plasticity destroy.
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http://dx.doi.org/10.1038/s41598-021-88206-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060272PMC
April 2021

MRI-based radiomics as response predictor to radiochemotherapy for metastatic cervical lymph node in nasopharyngeal carcinoma.

Br J Radiol 2021 Apr 21:20201212. Epub 2021 Apr 21.

Department of Radiology, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

Objective: To establish and substantiate MRI-based radiomic models to predict the treatment response of metastatic cervical lymph node to radiochemotherapy in patients with nasopharyngeal carcinoma (NPC).

Methods: A total of 145 consecutive patients with NPC were enrolled including 102 in primary cohort and 43 in validation cohort. Metastatic lymph nodes were diagnosed according to radiologic criteria and treatment response was evaluated according to the Response Evaluation Criteria in Solid Tumors. A total of 2704 radiomic features were extracted from contrast-enhanced -weighted imaging (CE-T1WI) and -weighted imaging (T2WI) for each patient, and were selected to construct radiomic signatures for CE-T1WI, T2WI, and combined CE-T1WI and T2WI, respectively. The area under curve (AUC) of receiver operating characteristic, sensitivity, specificity, and accuracy were used to estimate the performance of these radiomic models in predicting treatment response of metastatic lymph node.

Results: No significant difference of AUC was found among radiomic signatures of CE-T1WI, T2WI, and combined CE-T1WI and T2WI in the primary and validation cohorts (all > 0.05). For combined CE-T1WI and T2WI dataset, 12 features were selected to develop the radiomic signature. The AUC, sensitivity, specificity, and accuracy were 0.927 (0.878-0.975), 0.911 (0.804-0.970), 0.826 (0.686-0.922), and 0.872 (0.792-0.930) in primary cohort, and were 0.772 (0.624-0.920), 0.792 (0.578-0.929), 0.790 (0.544-0.939), and 0.791 (0.640-0.900) in validation cohort.

Conclusion: MRI-based radiomic models were developed to predict the treatment response of metastatic cervical lymph nodes to radiochemotherapy in patients with NPC, which might facilitate individualized therapy for metastatic lymph nodes before treatment.

Advances In Knowledge: Predicting the response in patients with NPC before treatment may allow more individualizing therapeutic strategy and avoid unnecessary side-effects and costs. Radiomic features extracted from metastatic cervical lymph nodes showed promising application for predicting the treatment response in NPC.
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http://dx.doi.org/10.1259/bjr.20201212DOI Listing
April 2021

sp. nov. and sp. nov., isolated from human faeces.

Int J Syst Evol Microbiol 2021 Apr;71(4)

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, PR China.

Four unknown strains, characterized as Gram-stain-negative, strictly anaerobic, non-motile and rod-shaped, were isolated from fresh faeces of healthy humans in PR China. Pairwise sequence comparisons of the 16S rRNA genes showed that these isolates were separated into two clusters. Cluster I (strains HF-5141 and HF-106) was most closely related to XB1A (98.0-98.3 % similarity) and ATCC 8483 (97.3-97.5 %), whereas cluster II (strains HF-5287 and HF-5300) exhibited a similarity range of 96.8-97.0 % to JCM 13345, 96.7-96.9 % to MAJ27 and 96.4-96.5 % to XB1A. The DNA G+C contents of type strains HF-5141 and HF-5287 were 41.5 and 42.6 mol%, respectively. These strains had anteiso-C as the major cellular fatty acid, MK-9 and MK-11 as the predominant respiratory quinones, and phosphatidylethanolamine, aminophospholipids and phospholipids as major polar lipids, which is typical for members of the genus . However, the average nucleotide identity and digital DNA-DNA hybridization values, accompanied by different phenotypic and biochemical characteristics, distinguished them from their corresponding closest relatives as well as from other recognized members of the genus . Therefore, strains HF-5141 and HF-5287 represent two novel species in the genus , for which the names sp. nov. and sp. nov. are proposed, with HF-5141 (=CGMCC 1.16787=GDMCC 1.1591=JCM 33480) and HF-5287 (=CGMCC 1.16724=GDMCC 1.1590=JCM 33481) as type strains.
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http://dx.doi.org/10.1099/ijsem.0.004772DOI Listing
April 2021

Measurement of Contact Angles at Carbon Fiber-Water-Air Triple-Phase Boundaries Inside Gas Diffusion Layers Using X-ray Computed Tomography.

ACS Appl Mater Interfaces 2021 May 21;13(17):20002-20013. Epub 2021 Apr 21.

Department of Chemical and Biomolecular Engineering, University of California, Irvine, Irvine, California 92697, United States.

Gas diffusion layers (GDLs) are porous carbonaceous layers that are widely used in energy conversion and storage devices. Simulation of water transport through GDLs, in a polymer electrolyte fuel cell (PEFC), for example, typically uses goniometer-measured external contact angles. Until now, there is no well-developed method to obtain contact angles inside the GDLs. AlRatrout et al. developed an open-source code to compute local contact angles at triple-phase contact points from segmented micro-X-ray computed tomography (X-ray CT) images of porous rocks. We apply it, for the first time, to micro-X-ray CT images of water-filled commercial GDLs and compute local contact angles at internal GDL fiber-water-air triple-phase contact points. We obtain a state of mixed wettability (hydrophilic and hydrophobic) inside all GDL samples, with a broad range of contact angles, instead of one hydrophobic contact angle found from goniometer experiments. Lattice Boltzmann water transport simulations performed with these distributed contact angles produce results that are in better agreement with experimental data. We also obtain high-resolution X-ray photoelectron spectroscopy (XPS) data of the GDL samples and find that the concentration of oxide species correlates strongly with the measured hydrophilicity. The method introduced here can help rationally design GDLs and directly quantify their internal surface wettability that is needed for accurate predictions of their functionality in energy technology devices.
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http://dx.doi.org/10.1021/acsami.1c00849DOI Listing
May 2021