Publications by authors named "Xinyue Xu"

35 Publications

Mesenchymal Stromal Cells Overexpressing Farnesoid X Receptor Exert Cardioprotective Effects Against Acute Ischemic Heart Injury by Binding Endogenous Bile Acids.

Adv Sci (Weinh) 2022 Jul 3:e2200431. Epub 2022 Jul 3.

Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, China.

Bile acid metabolites have been increasingly recognized as pleiotropic signaling molecules that regulate cardiovascular functions, but their role in mesenchymal stromal cells (MSC)-based therapy has never been investigated. It is found that overexpression of farnesoid X receptor (FXR), a main receptor for bile acids, improves the retention and cardioprotection of adipose tissue-derived MSC (ADSC) administered by intramyocardial injection in mice with myocardial infarction (MI), which shows enhanced antiapoptotic, proangiogenic, and antifibrotic effects. RNA sequencing, LC-MS/MS, and loss-of-function studies reveal that FXR overexpression promotes ADSC paracrine angiogenesis via Angptl4. FXR overexpression improves ADSC survival in vivo but fails in vitro. By performing bile acid-targeted metabolomics using ischemic heart tissue, 19 bile acids are identified. Among them, cholic acid and deoxycholic acid significantly increase Angptl4 secretion from ADSC overexpressing FXR and further improve their proangiogenic capability. Moreover, ADSC overexpressing FXR shows significantly lower apoptosis by upregulating Nqo-1 expression only in the presence of FXR ligands. Retinoid X receptor α is identified as a coactivator of FXR. It is first demonstrated that there is a bile acid pool in the myocardial microenvironment. Targeting the bile acid-FXR axis may be a novel strategy for improving the curative effect of MSC-based therapy for MI.
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http://dx.doi.org/10.1002/advs.202200431DOI Listing
July 2022

Mechanochemical Divergent Syntheses of Oxindoles and α-Arylacylamides via Controllable Construction of C-C and C-N Bonds by Copper and Piezoelectric Materials.

Angew Chem Int Ed Engl 2022 Jun 28:e202206420. Epub 2022 Jun 28.

Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, 325001, China.

Herein, we present the divergent syntheses of α-arylacylamides and oxindoles via mechanoredox chemistry by using easily accessible α-bromo N-sulfonyl amides as starting materials. Our system consists of a catalytic amount of Cu precatalyst and piezoelectric materials. The highly polarized BaTiO or PbTiO under mechanical agitation can act as an electron donor to realize the recycling of Cu and Cu . Control experiments and density functional theory calculations have been performed to support the proposed mechanistic rationale for the cascade reactions.
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http://dx.doi.org/10.1002/anie.202206420DOI Listing
June 2022

Liddle syndrome misdiagnosed as primary aldosteronism is caused by inaccurate aldosterone-rennin detection while a novel mutation is discovered.

Blood Press 2022 12;31(1):139-145

Department of Endocrinology, Shanghai Public Health Clinical Center, Shanghai, China.

Purpose: Through describing the confusing misdiagnosis process of Liddle syndrome, we try to reveal the importance of accurate aldosterone-renin detection and a genetic test for Liddle syndrome.

Methods: We found a family of hypertension and hypokalaemia with the proband of a 21-year-old female who had been misdiagnosed as primary aldosteronism (PA). She presented with high aldosterone and low renin levels. Aldosterone is not suppressed in the saline infusion test and captopril challenge test. However, treatment with a standard dose of spironolactone has no blood pressure improvement effect. A heterozygous variant of was found with whole exome sequencing and Liddle syndrome is indicated. Treatment with amiloride was effective. We rechecked aldosterone-renin levels with two different aldosterone and renin test kits. Clinical features and the mutant gene SCNN1G of each family member were determined by the Sanger method.

Results: The two kits had nearly opposite results. Among those Liddle syndrome patients confirmed by a genetic test, for Test kit A all ARR were screened positive while for test kit B negative. It seems Test kit B is consistent with the diagnosis while test kit A misleads the diagnosis. A novel mutation, c.1729 C > T, was found in this family, which introduce a premature stop codon in the γ subunit in the epithelial Na channel (ENaC) and resulted in a deletion of 72 amino acids at the carboxyl end.

Conclusion: inaccurate ARR detection might misdiagnose Liddle syndrome. A Gene test is an important method for the diagnosis of Liddle syndrome. A novel missense mutation, c.1729 C > T, is found in a Chinese family.
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http://dx.doi.org/10.1080/08037051.2022.2088471DOI Listing
December 2022

Improved Conventional and New Approaches in the Diagnosis of Tuberculosis.

Front Microbiol 2022 31;13:924410. Epub 2022 May 31.

West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China.

Tuberculosis (TB) is a life-threatening infectious disease caused by . Timely diagnosis and effective treatment are essential in the control of TB. Conventional smear microscopy still has low sensitivity and is unable to reveal the drug resistance of this bacterium. The traditional culture-based diagnosis is time-consuming, since usually the results are available after 3-4 weeks. Molecular biology methods fail to differentiate live from dead , while diagnostic immunology methods fail to distinguish active from latent TB. In view of these limitations of the existing detection techniques, in addition to the continuous emergence of multidrug-resistant and extensively drug-resistant TB, in recent years there has been an increase in the demand for simple, rapid, accurate and economical point-of-care approaches. This review describes the development, evaluation, and implementation of conventional diagnostic methods for TB and the rapid new approaches for the detection of .
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http://dx.doi.org/10.3389/fmicb.2022.924410DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9195135PMC
May 2022

Does cooperation stimulate firms' eco-innovation? Firm-level evidence from China.

Environ Sci Pollut Res Int 2022 Jun 11. Epub 2022 Jun 11.

School of Government, Central University of Finance and Economics, Beijing, 100081, China.

Firms' collaborative activities have created increasing opportunities for eco-innovation in modern society. Based on unbalanced panel data from the Chinese National Innovation Survey between 2011 and 2015, this paper explored the influences of different modes of cooperation, i.e., vertical cooperation, horizontal cooperation, and mixed cooperation, on the eco-innovation of Chinese manufacturing firms. The results indicated that three types of cooperation all had positive and statistically significant effects on the firms' eco-innovation, and mixed cooperation had promoted eco-innovation more dramatically. The extent of such impacts may vary depending on the heterogeneity of the characteristic of enterprises. We also verified that knowledge spillovers from cooperative partners have played a mediating role between cooperation and eco-innovation. Our results suggest the potential benefits of diversified collaborative activities and appropriate intellectual property protection for firms' eco-innovation in China.
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http://dx.doi.org/10.1007/s11356-022-21296-6DOI Listing
June 2022

Emulsifying properties of different soy hull pectin polysaccharides and application in mayonnaise.

Food Sci Biotechnol 2022 Jun 22;31(6):699-710. Epub 2022 Apr 22.

Technology Department, College of Food Science and Technology, Bohai University, 19 Keji Road, Jinzhou, 121013 Liaoning People's Republic of China.

Soy hull pectic polysaccharide (SHPP) is a kind of biological macromolecule prepared from soy hull, which has certain thickening and gel properties. In present study, SHPP was extracted with citric acid and sodium citrate from soybean hulls under the assistance of microwave, respectively. SHPPs were then compared with commercial pectin polysaccharide to test their emulsification ability. The emulsion prepared from SHPP extracted with sodium citrate has the best emulsifying effect, small particle size and uniform distribution. The rheological properties and particle size distribution of mayonnaise did not change significantly after the addition of different SHPPs and commercial pectin polysaccharides. However, microscopic observations revealed that the droplets were more uniformly distributed in the mayonnaise after the addition of SHPP extracted with sodium citrate under the assistance of microwave and commercial pectin APC141. SHPP extracted with sodium citrate may play an important role in maintaining emulsion stability in the future.

Supplementary Information: The online version of this article (10.1007/s10068-022-01083-2) contains supplementary material, which is available to authorized users.
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http://dx.doi.org/10.1007/s10068-022-01083-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133284PMC
June 2022

Effects of different combinations of probiotics on rheology, microstructure, and moisture distribution of soy materials-based yogurt.

J Food Sci 2022 Jul 31;87(7):2820-2830. Epub 2022 May 31.

College of Food Science and Technology, Bohai University, Jinzhou, China.

The effects of different probiotics on the texture, rheological properties, microstructure, and water distribution of yogurt fermented with soy powder, soy isolate protein powder, soy umbilical powder, and soy whey as the main raw materials were studied. The soy materials-based yogurt fermented by Danisco mixed probiotic reached the end of fermentation after 4 h, which significantly shortened the fermentation time compared with other probiotic combinations. The fermentation with Danisco mixed probiotic and Kefir mixed probiotic respectively resulted in good texture and a denser and more homogeneous microstructure, which was consistent with the sensory evaluation results. Both fermentations had a high water holding capacity of 90.92% and 78.30%, respectively, in agreement with the results of moisture distribution tests. However, the elastic and viscous behaviors were weaker at certain shear frequency. This study achieved a high value-added utilization of soy whey and the development of a new soy materials-based yogurt that met the consumption needs of people with lactose intolerance and high cholesterol. PRACTICAL APPLICATION: In this study, high value-added utilization of soy whey was realized, which solved the problems of resource waste and environmental pollution. Meanwhile, the research and development of soy materials-based yogurt provided another nutritional and healthy consumption demand for lactose intolerant people.
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http://dx.doi.org/10.1111/1750-3841.16204DOI Listing
July 2022

Functional analysis of nuclear receptor HR96 gene in Bombyx mori exposed to phoxim.

Arch Insect Biochem Physiol 2022 Apr 25:e21910. Epub 2022 Apr 25.

School of Life Science, Anhui Agricultural University, Hefei, China.

The nuclear receptor (NRs) gene family functions as ligand-dependent transcription factors in a variety of animals, which participates in a variety of biological processes, such as cell differentiation, metabolic regulation, reproduction, development, insect metamorphosis. In this study, a nuclear receptor HR96 gene in silkworm Bombyx mori (BmHR96) was identified, and the responses of BmHR96 gene to 20-hydroxyecdysone (20E), three insecticides, and two disinfectants were analyzed and its function in phoxim exposure was explored.  Quantitative real-time polymerase chain reaction indicated that the expression of BmHR96 mRNA was the highest in ovary of 5th instar Day 3 silkworm larvae and in silk gland of the wandering stage. The expression patterns of BmHR96 gene in ovary, head, testis, and midgut of different stages were different. After injecting 20E into B. mori, the expression of BmHR96 mRNA had no significant difference compared with control. Three insecticides and two disinfectants were used to treat B. mori, respectively, and it was found that they had different influence patterns on the expression level of BmHR96. siRNA of BmHR96 was injected into silkworm larvae and the expression of BmHR96 was decreased significantly after injecting 72 h. After silencing of BmHR96, B. mori was fed with phoxim-treated leaves. The results showed that the mortality of B. mori after silencing of BmHR96 was significantly higher than the control. Our results indicated that HR96 plays an important role in regulating the stress response of phoxim.
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http://dx.doi.org/10.1002/arch.21910DOI Listing
April 2022

Elevated AST/ALT ratio is associated with all-cause mortality and cancer incident.

J Clin Lab Anal 2022 May 22;36(5):e24356. Epub 2022 Mar 22.

Department of Non-Communicable Chronic Disease Control and Prevention, Taizhou Center for Disease Control and Prevention, Taizhou, Zhejiang Province, China.

Background: The aspartate transaminase (AST)-to-alanine aminotransferase (ALT) ratio, which is used to measure liver injury, has been found to be associated with some chronic diseases and mortality. However, its relevance to cancer incidence resulting from population-based prospective studies has rarely been reported. In this study, we investigated the correlation of the AST/ALT ratio as a possible predictor of mortality and cancer incidence.

Methods: A total of 9,946 participants fulfilled the inclusion criteria for a basic public health service project of the Health Checkup Program conducted by the BaiYun Community Health Service Center, Taizhou. Deceased participants and cancer incident cases were from The Taizhou Chronic Disease Information Management System. Odds ratios (ORs) and interval of quartile range (IQR) computed by logistic regression analysis and cumulative incidence rate were calculated by the Kaplan-Meier survival method and compared with log-rank test statistics.

Results: Serum ALT and AST levels were both increased in patients with chronic diseases, but the ratio of AST/ALT was generally decreased. The cancer incident cases (488 new cases) had a greater baseline ratio (median =1.23, IQR: 0.96-1.54) than noncancer cases (median =1.15, IQR: 0.91-1.44). Compared to the first quartile of the AST/ALT ratio, the population in the top quartile had a higher cumulative cancer incidence rate (7.54% vs. 4.44%) during follow-up period. Furthermore, an elevated AST/ALT ratio increased the risk of all-cause mortality.

Conclusions: The ratio of AST/ALT is a potential biomarker to assess healthy conditions and long-term mortality. Especially for cancer, the AST/ALT ratio not only increases at baseline but also predicts the future development of cancer. The clinical value and potential mechanism deserve further research.
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http://dx.doi.org/10.1002/jcla.24356DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102513PMC
May 2022

Clinical Potential of Hypoxia Inducible Factors Prolyl Hydroxylase Inhibitors in Treating Nonanemic Diseases.

Front Pharmacol 2022 24;13:837249. Epub 2022 Feb 24.

Department of Nephrology, Children's Hospital of Nanjing Medical University, Nanjing, China.

Hypoxia inducible factors (HIFs) and their regulatory hydroxylases the prolyl hydroxylase domain enzymes (PHDs) are the key mediators of the cellular response to hypoxia. HIFs are normally hydroxylated by PHDs and degraded, while under hypoxia, PHDs are suppressed, allowing HIF-α to accumulate and transactivate multiple target genes, including erythropoiesis, and genes participate in angiogenesis, iron metabolism, glycolysis, glucose transport, cell proliferation, survival, and so on. Aiming at stimulating HIFs, a group of small molecules antagonizing HIF-PHDs have been developed. Of these HIF-PHDs inhibitors (HIF-PHIs), roxadustat (FG-4592), daprodustat (GSK-1278863), vadadustat (AKB-6548), molidustat (BAY 85-3934) and enarodustat (JTZ-951) are approved for clinical usage or have progressed into clinical trials for chronic kidney disease (CKD) anemia treatment, based on their activation effect on erythropoiesis and iron metabolism. Since HIFs are involved in many physiological and pathological conditions, efforts have been made to extend the potential usage of HIF-PHIs beyond anemia. This paper reviewed the progress of preclinical and clinical research on clinically available HIF-PHIs in pathological conditions other than CKD anemia.
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http://dx.doi.org/10.3389/fphar.2022.837249DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8908211PMC
February 2022

Enhancing patient-centred communication across barriers: The case of intersubjectivity management in medical interpreting.

Patient Educ Couns 2022 07 19;105(7):2012-2018. Epub 2021 Nov 19.

Faculty of Education, The University of Hong Kong, Hong Kong, China. Electronic address:

Objective: This study introduces the concept of intersubjectivity management in medical interpreting and identifies relevant interactional strategies employed by the interpreter, also explores their effectiveness in facilitating positive clinician-patient communication.

Methods: We used conversation analysis (CA) to analyse 27 video recordings of interpreter-mediated dental visits, participants involve English-speaking dentists, Cantonese as the first language (L1) patients and bilingual dental surgery assistants (DSA) who also play the role of ad hoc interpreters.

Results: The DSA-as-interpreter manages intersubjectivity for the dentist and patient through interactional strategies, such as reformulating action types, redesigning contents and information capacity, summarising and concentrating turns, constantly monitoring the situation and eliciting spoken or unspoken expressions that are medically relevant from both sides to validate them. The strategies effectively enabled and enhanced the mutual understanding and interpersonal alignment between the dentist and patient. More importantly, the DSA constantly orients to patient-centred communication.

Conclusion: Although not professionally trained for interpreting, the DSA-as-interpreters demonstrated discursive strategies. The strategies evidently facilitated positive dentist-patient communication and relationships.

Practice Implications: The conceptualisation and significant strategies demonstrated by the DSA-as-interpreters could potentially inform the solution of enhancing multilingual health communication in clinical staff training.
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http://dx.doi.org/10.1016/j.pec.2021.11.006DOI Listing
July 2022

Designing and fabricating a CdS QDs/BiMoO monolayer S-scheme heterojunction for highly efficient photocatalytic CH degradation under visible light.

J Hazard Mater 2022 Feb 6;424(Pt D):127685. Epub 2021 Nov 6.

Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, School of Materials Science & Engineering, Hubei University, Wuhan 430062, China. Electronic address:

Achieving efficient photocatalytic degradation of atmospheric volatile organic compounds (VOCs) under sun-light is still a significant challenge for environmental protection. The S-scheme heterojunction with its unique charge migration route, high charge separation rate and strong redox ability, has great potential. However, how to regulate interfacial charge transfer of the S-scheme heterojunction is of significant importance. Here, density functional theory (DFT) calculations were first conducted and predicted that an S-scheme heterojunction could be formed in the CdS quantum dots/BiMoO monolayer system. Subsequently, this novel heterojunction is constructed by in-situ hydrothermal synthesis of CdS quantum dots on monolayer BiMoO. Under visible-light, this novel S-scheme system gives a high-efficiency photocatalytic degradation rate (6.04 × 10 min) towards CH, which is 30.3 times higher than that of pure CdS (1.99 × 10 min) and 41.7 times higher than pure BiMoO (1.45 × 10 min). Strong evidence for the S-scheme charge transfer path is provided by in-situ XPS, PL, TRPL and EPR.
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http://dx.doi.org/10.1016/j.jhazmat.2021.127685DOI Listing
February 2022

Anatase and Rutile TiO Nanoparticles Lead Effective Bone Damage in Young Rat Model via the IGF-1 Signaling Pathway.

Int J Nanomedicine 2021 27;16:7233-7247. Epub 2021 Oct 27.

Department of Pediatrics, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, 330006, People's Republic of China.

Purpose: To evaluate the effects of anatase and rutile TiO nanoparticles (NPs) on the growth and development of bones in young rats and explore their possible mechanisms.

Methods: Three-week-old male rats were orally administered anatase TiO NPs and rutile TiO NPs for 28 days. The indicators of rat growth and development, liver function, bone metabolism, and insulin-like growth factor-1 (IGF-1) levels were evaluated. Micro-computed tomography (micro-CT) and immunohistochemistry were used to evaluate the tibia.

Results: No significant differences were observed among growth and development indicators in young rats. Significant differences were found in IGF-1 levels, phosphorus levels, and liver function. Micro-CT revealed osteoporosis in the bones. The micro-CT data supported the same result. Bone immunohistochemistry results showed that the expression of osteoprotegerin (OPG) was decreased and the expression of receptor activator of nuclear factor-κB ligand (RANKL) and cathepsin K (CTSK) was increased.

Conclusion: This study demonstrated that TiO NPs can damage bones via the IGF-1/OPG/RANKL/CTSK pathway in young rats. Furthermore, rutile TiO NPs damaged the bones more seriously than anatase TiO NPs.
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http://dx.doi.org/10.2147/IJN.S333632DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8558833PMC
November 2021

Lithography-free synthesis of periodic, vertically-aligned, multi-walled carbon nanotube arrays.

Nanotechnology 2021 Nov 18;33(6). Epub 2021 Nov 18.

International Academy of Optoelectronics at Zhaoqing, South China Normal University, Zhaoqing, 526238 Guangdong, People's Republic of China.

Until now, the growth of periodic vertically aligned multi-walled carbon nanotube (VA-MWCNT) arrays was dependent on at least one lithography step during fabrication. Here, we demonstrate a lithography-free fabrication method to grow hexagonal arrays of self-standing VA-MWCNTs with tunable pitch and MWCNT size. The MWCNTs are synthesized by plasma enhanced chemical vapor deposition (PECVD) from Ni catalyst particles. Template guided dewetting of a thin Ni film on a hexagonally close-packed silica particle monolayer provides periodically distributed Ni catalyst particles as seeds for the growth of the periodic MWCNT arrays. The diameter of the silica particles directly controls the pitch of the periodic VA-MWCNT arrays from 600 nm to as small as 160 nm. The diameter and length of the individual MWCNTs can also be readily adjusted and are a function of the Ni particle size and PECVD time. This unique method of lithography-free growth of periodic VA-MWCNT arrays can be utilized for the fabrication of large-scale biomimetic materials.
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http://dx.doi.org/10.1088/1361-6528/ac345aDOI Listing
November 2021

VizLinter: A Linter and Fixer Framework for Data Visualization.

IEEE Trans Vis Comput Graph 2022 Jan 28;28(1):206-216. Epub 2021 Dec 28.

Despite the rising popularity of automated visualization tools, existing systems tend to provide direct results which do not always fit the input data or meet visualization requirements. Therefore, additional specification adjustments are still required in real-world use cases. However, manual adjustments are difficult since most users do not necessarily possess adequate skills or visualization knowledge. Even experienced users might create imperfect visualizations that involve chart construction errors. We present a framework, VizLinter, to help users detect flaws and rectify already-built but defective visualizations. The framework consists of two components, (1) a visualization linter, which applies well-recognized principles to inspect the legitimacy of rendered visualizations, and (2) a visualization fixer, which automatically corrects the detected violations according to the linter. We implement the framework into an online editor prototype based on Vega-Lite specifications. To further evaluate the system, we conduct an in-lab user study. The results prove its effectiveness and efficiency in identifying and fixing errors for data visualizations.
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http://dx.doi.org/10.1109/TVCG.2021.3114804DOI Listing
January 2022

Luteolin alleviates inorganic mercury-induced kidney injury via activation of the AMPK/mTOR autophagy pathway.

J Inorg Biochem 2021 11 19;224:111583. Epub 2021 Aug 19.

College of Veterinary Medicine, Northeast Agricultural University, 600 Changjiang Road, Harbin 150030, China; Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, 600 Changjiang Road, Harbin 150030, China. Electronic address:

Inorganic mercury is a ubiquitous toxic pollutant in the environment. Exposure to inorganic mercury can cause various poisonous effects, including kidney injury. However, no safe and effective treatment for kidney injury caused by inorganic mercury has been found and used. Luteolin (Lut) possesses various beneficial bioactivities. Here, our research aims to investigate the protective effect of Lut on renal injury induced by mercury chloride (HgCl) and identify the underlying autophagy regulation mechanism. Twenty-eight 6-8 weeks old Wistar rats were randomly assigned to four groups: control, HgCl, HgCl + Lut, and Lut. We performed the determination of oxidative stress and renal function indicators, histopathological analysis, the terminal deoxynucleotidyl transferase-mediated deoxyuracil nucleoside triphosphate nick-end labeling assay to detect apoptosis, western blot detection of autophagy-related protein levels, and atomic absorption method to detect mercury content. Our results showed that Lut ameliorated oxidative stress, apoptosis and restored the autophagy and renal function caused by HgCl in rats. Concretely, the level of nuclear factor E2-related factor, renal adenosine monophosphate-activated protein kinase (AMPK) expression, and autophagy regulation-related proteins levels were down-regulated, and the mammalian target of rapamycin (mTOR) expression was up-regulated by HgCl treatment. However, Lut treatment reversed the above changes. Notably, Lut reduced the accumulation of HgCl in the kidneys and promoted the excretion of HgCl through urine. Collectively, our results demonstrate that Lut can attenuate inorganic mercury-induced renal injury via activating the AMPK/mTOR autophagy pathway. Therefore, Lut may be a potential biological medicine to protect against renal damage induced by HgCl.
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http://dx.doi.org/10.1016/j.jinorgbio.2021.111583DOI Listing
November 2021

Structure/function relationships of bean polysaccharides: A review.

Crit Rev Food Sci Nutr 2021 Jul 13:1-15. Epub 2021 Jul 13.

College of Food Science and Technology, Bohai University, Jinzhou, Liaoning, China.

Beans are a rich source of high quality protein and oil, and have attracted increasing interest from both nutrition researchers and health-conscious consumers. This review aims to provide a foundation for the future research and development of bean polysaccharides, by summarizing the sources, structure, and functions of bioactive bean polysaccharides. Structure/function relationships are described, for biological activities, such as immunological, antioxidant and anti-diabetes. This will provide useful guidance for further optimization of polysaccharide structure and the development of bean polysaccharides as a novel functional material.
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http://dx.doi.org/10.1080/10408398.2021.1946480DOI Listing
July 2021

Effect of SGLT2 Inhibitors on Type 2 Diabetes Mellitus With Non-Alcoholic Fatty Liver Disease: A Meta-Analysis of Randomized Controlled Trials.

Front Endocrinol (Lausanne) 2021 17;12:635556. Epub 2021 Jun 17.

Department of Endocrinology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, China.

Objective: Clinical trials showed that sodium-glucose cotransporter 2 (SGLT2) inhibitors can improve non-alcoholic fatty liver disease (NAFLD). In this work, a meta-analysis of randomized controlled trials was conducted to evaluate the effect of SGLT2 inhibitors on type 2 diabetes mellitus (T2DM) with NAFLD.

Methods: PubMed, Embase, Web of Science, and Cochrane Libraries were used for the systematic literature review to determine eligible studies. A randomized effect model was adapted to perform a meta-analysis on these eligible studies to estimate the combined effect sizes. Differences were expressed as the weighted average difference (WMD) of the continuous results and the 95% confidence interval (CI).

Results: Ten randomized controlled trials with 573 participants were included. SGLT2 inhibitors significantly reduced the levels of alanine transaminase (WMD -5.36 [95% CI: -8.86, -1.85], p = 0.003) and Aspartate Transaminase (WMD -2.56 [95% CI: -3.83, -1.29], p <0.0001). In terms of body composition, liver proton density fat fraction (WMD -2.20 [95% CI: -3.67, -0.74], p = 0.003), visceral fat mass area (WMD -20.71 [95% CI: -28.19, -13.23], p <0.00001), subcutaneous fat areas (WMD -14.68 [95% CI: -26.96, -2.40], p = 0.02) were also significantly reduced.

Conclusion: SGLT2 inhibitors can remarkably reduce hepatic enzymes, hepatic fat and improve body composition. Thus, they may become a new treatment option for NAFLD.

Systematic Review Registration: PROSPERO, identifier CRD42020215570.
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http://dx.doi.org/10.3389/fendo.2021.635556DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247927PMC
January 2022

Suppression of CD13 Enhances the Cytotoxic Effect of Chemotherapeutic Drugs in Hepatocellular Carcinoma Cells.

Front Pharmacol 2021 11;12:660377. Epub 2021 May 11.

School of Pharmacy, Weifang Medical University, Weifang, China.

Multidrug resistance (MDR) of hepatocellular carcinoma (HCC) is a serious problem that directly hinders the effect of chemotherapeutics. In this study, we mainly explore the molecular mechanism of ROS-induced CD13 expression using hepatocarcinoma cells as the research object. We show that the drug of fluorouracil (5FU), epirubicin (EPI) and gemcitabine (GEM) can induce ROS generation, activate Ets2 and promote CD13 expression. Meanwhile, CD13 can activate NRF1 and up-regulate ROS scavenging genes transcription, such as SOD1, GPX1, GPX2 and GPX3, leading to down-regulation of intracellular ROS level and reducing the sensitivity of cells to chemotherapy agent. We also detected the anti-tumor effect of the combination therapy, CD13 inhibitor ubenimex and a variety of conventional anti-cancer drugs, such as 5FU, EPI, GEM, pemetrexed (Pem) and paclitaxel (PTX) were employed in combination. Ubenimex enhances the sensitivity of different chemotherapeutic agents and cooperates with chemotherapeutic agents to suppress tumor growth and . In general, overexpression of CD13 can lead to chemotherapy resistance, and CD13 inhibitor can reverse this effect. Combination of chemotherapy agent and ubenimex will become a potential treatment strategy for liver cancer resistance.
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http://dx.doi.org/10.3389/fphar.2021.660377DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144446PMC
May 2021

Novel quantitative structure-activity relationship model to predict activities of natural products against COVID-19.

Chem Biol Drug Des 2021 04 17;97(4):978-983. Epub 2021 Jan 17.

Department of Chemistry, Lanzhou University, Lanzhou, China.

Currently, COVID-19 is spreading in a large scale while no efficient vaccine has been produced. A high-effective drug for COVID-19 is very necessary now. We established a satisfied quantitative structure-activity relationship model by gene expression programming to predict the IC50 value of natural compounds. A total of 27 natural products were optimized by heuristic method in CODESSA program to build a liner model. Based on it, only two descriptors were selected and utilized to build a nonlinear model in gene expression programming. The square of correlation coefficient and s2 of heuristic method were 0.80 and 0.10, respectively. In gene expression programming, the square of correlation coefficient and mean square error for training set were 0.91 and 0.04. The square of correlation coefficient and mean square error for test set are 0.86 and 0.1. This nonlinear model has stronger predictive ability to develop the targeted drugs of COVID-19.
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http://dx.doi.org/10.1111/cbdd.13822DOI Listing
April 2021

Oral Exposure to ZnO Nanoparticles Disrupt the Structure of Bone in Young Rats via the OPG/RANK/RANKL/IGF-1 Pathway.

Int J Nanomedicine 2020 3;15:9657-9668. Epub 2020 Dec 3.

Department of Pediatrics, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, People's Republic of China.

Purpose: To evaluate the effects of ZnO NPs on bone growth in rats and explore the possible mechanisms of action.

Materials And Methods: Three-week-old male rats received ultrapure water or 68, 203, and 610 mg/kg zinc oxide nanoparticles (ZnO NPs) for 28 days, orally.

Results: The high-dosage groups caused significant differences in weight growth rate, body length, and tibia length (P<0.05), all decreasing with increased ZnO NP dosage. There were no significant differences in body mass index (BMI) (P>0.05). The zinc concentration in liver and bone tissue increased significantly with increased ZnO NP dosage (P<0.05). Clearly increased aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were observed in the 610 mg/kg ZnO NP group (P>0.05), whereas alkaline phosphatase (ALP) increased in the 610 mg/kg ZnO NP group (P<0.05). Significant differences in insulin-like growth factor type 1 (IGF-1) levels and a decrease in calcium (Ca) levels were observed in 203 and 610 mg/kg ZnO NP groups (P<0.05). Phosphorus (P) levels increased and the Ca/P ratio decreased in the 610 mg/kg ZnO NP group (P<0.05). Micro-computed tomography (micro-CT) of the tibia demonstrated signs of osteoporosis, such as decreased bone density, little trabecular bone structure and reduced cortical bone thickness. Micro-CT data further demonstrated significantly decreased bone mineral density (BMD), trabecular number (Tb.N), and relative bone volume (BV/TV) with increasing dosage of ZnO NPs. Osteoprotegerin (OPG) expression and the ratio of OPG to receptor activator of nuclear factor-κB ligand (RANKL) were statistically lower in the 610 mg/kg ZnO NP group (P<0.05), whereas RANKL expression did not change significantly (P>0.05).

Conclusion: We infer that ZnO NPs affect bone growth in young rats directly or indirectly by altering IGF-1 levels. Overall, the results indicate that ZnO NPs promote osteoclast activity and increase bone loss through the OPG/RANK/RANKL/IGF-1 pathway.
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http://dx.doi.org/10.2147/IJN.S275553DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7721121PMC
December 2020

LBBAP in patients with normal intrinsic QRS duration: Electrical and mechanical characteristics.

Pacing Clin Electrophysiol 2021 01 12;44(1):82-92. Epub 2021 Jan 12.

Department of Cardiology, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing, China.

Background: Left bundle branch area pacing (LBBAP) is an innovative pacing technology, which needs further study.

Methods: Seventy LBBAP patients with intrinsic QRS duration (QRSd) less than 120 ms were consecutively enrolled in our center. According to whether the left bundle branch potential (LBBp) was recorded or not, the patients were divided into the potential positive group (LBBAP+) and the potential negative group (LBBAP-). Electrocardiographic and echocardiographic parameters were used to evaluate electrical and mechanical characteristics. Lead parameters and complications were followed-up.

Results: There were 52 patients in LBBAP+ and 18 patients in LBBAP-. The QRSd and the left ventricular activation time (LVAT) were wider after LBBAP. QRSd showed no significant difference between LBBAP+ and LBBAP-. LVAT was significantly shorter in LBBAP+ than in LBBAP-. Frontal QRS axis shifted leftward and the V1 morphologies changed after LBBAP. QRS axis and V1 morphologies showed no significant differences between two groups. Paced R-wave transition moved forward compared with intrinsic R-wave transition in both groups. Peak systolic strain of left ventricle (LVPSS) increased, and peak systolic dispersion of left ventricle (LVPSD) did not change significantly after LBBAP. Systolic and diastolic function as well as mechanical synchronism had no significant differences between two groups. LBBAP had great pacing parameters.

Conclusion: LBBAP changes electrical and mechanical characteristics and has good safety in patients with normal intrinsic QRSd. LBBAP+ and LBBAP- show no significant differences in mechanical synchronization and interventricular electrical synchronization. The LBBAP+ shows better left ventricular electrical synchronicity.
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http://dx.doi.org/10.1111/pace.14114DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7898286PMC
January 2021

Calliope: Automatic Visual Data Story Generation from a Spreadsheet.

IEEE Trans Vis Comput Graph 2021 Feb 28;27(2):453-463. Epub 2021 Jan 28.

Visual data stories shown in the form of narrative visualizations such as a poster or a data video, are frequently used in data-oriented storytelling to facilitate the understanding and memorization of the story content. Although useful, technique barriers, such as data analysis, visualization, and scripting, make the generation of a visual data story difficult. Existing authoring tools rely on users' skills and experiences, which are usually inefficient and still difficult. In this paper, we introduce a novel visual data story generating system, Calliope, which creates visual data stories from an input spreadsheet through an automatic process and facilities the easy revision of the generated story based on an online story editor. Particularly, Calliope incorporates a new logic-oriented Monte Carlo tree search algorithm that explores the data space given by the input spreadsheet to progressively generate story pieces (i.e., data facts) and organize them in a logical order. The importance of data facts is measured based on information theory, and each data fact is visualized in a chart and captioned by an automatically generated description. We evaluate the proposed technique through three example stories, two controlled experiments, and a series of interviews with 10 domain experts. Our evaluation shows that Calliope is beneficial to efficient visual data story generation.
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http://dx.doi.org/10.1109/TVCG.2020.3030403DOI Listing
February 2021

Functional analysis of Dicer-2 gene in Bombyx mori resistance to BmNPV virus.

Arch Insect Biochem Physiol 2020 Sep 5;105(1):e21724. Epub 2020 Jul 5.

School of Life Science, Anhui Agricultural University, Hefei, China.

Bombyx mori nucleopolyhedrovirus (BmNPV) is a primary pathogen in silkworm, and the molecular mechanism of B. mori defense to BmNPV infection is still unclear. RNA interference (RNAi) is well-known as an intracellular conserved mechanism that is critical in gene regulation and cell defense. The antiviral RNAi pathway processes viral double-stranded RNA (dsRNA) into viral small interfering RNAs that guide the recognition and cleavage of complementary viral target RNAs. In this study, a Dicer-2 (Dcr2) gene was identified in B. mori and its antiviral function was explored. Dcr2 messenger RNA (mRNA) expression was the highest in hemocytes and expressed in all stages of silkworm growth. After infection with BmNPV, the expression of Dcr2 mRNA was significantly increased after infection in midgut and hemocytes. The expression of Dcr2 was significantly upregulated by injecting dsRNA (dsBmSPH-1) into silkworm after 48 hr. Knocking down the expression level of Dcr2 using specific dsRNA in silkworm, which modestly enhanced the production of viral genomic DNA. Our results suggested that the Dcr2 gene in B. mori plays an important role in against BmNPV invasion.
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http://dx.doi.org/10.1002/arch.21724DOI Listing
September 2020

Vimentin plays an important role in the promotion of breast cancer cell migration and invasion by leucine aminopeptidase 3.

Cytotechnology 2020 Oct 22;72(5):639-647. Epub 2020 Jun 22.

School of Pharmacy, Weifang Medical University, Weifang, China.

Breast cancer is a common type of cancer in females. Our previous studies indicated that leucine aminopeptidase 3 (LAP3) promotes migration and invasion of breast cancer cells. Vimentin is a mesenchymal marker, and its upregulation represents the promotion of epithelial-mesenchymal transition. In this study, we found that LAP3 and vimentin were highly expressed in breast cancer tissues, and the overexpression of LAP3 in breast cancer cells promoted the expression of vimentin. Western blot analysis indicated that the overexpression of LAP3 upregulated the phosphorylation of Erk1/2. MEK inhibitor PD98059 downregulated the expression of vimentin, matrix metalloproteinase-2/9 (MMP-2/9), and fascin through the inhibition of Erk1/2 activity. We hypothesized that LAP3 promoted tumor migration and invasion by upregulating vimentin. The knockdown of vimentin resulted in the inhibited migration and invasion of MDA-MB-231 and MDA-MB-468 cells. The expression of MMP-2/9 and fascin could also be downregulated. In conclusion, vimentin might play an important role in the promotion of breast cancer metastasis by LAP3.
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http://dx.doi.org/10.1007/s10616-020-00402-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7548013PMC
October 2020

CD13 Induces Autophagy to Promote Hepatocellular Carcinoma Cell Chemoresistance Through the P38/Hsp27/CREB/ATG7 Pathway.

J Pharmacol Exp Ther 2020 09 22;374(3):512-520. Epub 2020 Jun 22.

School of Pharmacy, Weifang Medical University, Weifang, Shandong, China (Y.Z., H.W., X.Xi., Y.M., S.J., X.Xu., X.Z., S.W., W.J., C.F., L.Z., F.Y., X.W.) and Department of pharmacy, Southwestern Lu Hospital, Liaocheng, Shandong, China (H.W.)

The chemoresistance of hepatocellular carcinoma (HCC) is a serious problem that directly hinders the effect of chemotherapeutic agents. We previously reported that Aminopeptidase N (CD13) inhibition can enhance the cytotoxic efficacy of chemotherapy agents. In the present study, we use liver cancer cells to explore the molecular mechanism accounting for the relationship between CD13 and chemoresistance. We demonstrate that CD13 overexpression activates the P38/heat shock protein 27/cAMP response element-binding protein (CREB) signaling pathway to limit the efficacy of cytotoxic agents. Moreover, blockade of P38 or CREB sensitizes HCC cells to 5-fluorouracil. Then we reveal that CREB binds to the autophagy related 7 () promoter to induce autophagy and promote HCC cell chemoresistance. CD13 inhibition also downregulates the expression of ATG7, autophagy, and tumor cell growth in vivo. Overall, the combination a CD13 inhibitor and chemotherapeutic agents may be a potential strategy for overcoming drug resistance in HCC. SIGNIFICANCE STATEMENT: Our study demonstrates that Aminopeptidase N (CD13) promotes hepatocellular carcinoma (HCC) cell chemoresistance via the P38/heat shock protein 27/cAMP response element-binding protein (CREB) pathway. CREB regulates autophagy related 7 transcription and expression to induce autophagy. Our results collectively suggest that CD13 may serve as a potential target for overcoming HCC resistance.
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http://dx.doi.org/10.1124/jpet.120.265637DOI Listing
September 2020

Oral exposure of titanium oxide nanoparticles induce ileum physical barrier dysfunction via Th1/Th2 imbalance.

Environ Toxicol 2020 Sep 25;35(9):982-990. Epub 2020 Apr 25.

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China.

In this work, we aimed to evaluate the adverse effects and the mechanism of intestinal barrier caused by titanium dioxide nanoparticles (TiO NPs). Here, the effects of two different dosages (300 and 1200 mg/kg) of TiO NPs on female mice (n = 5) were investigated. After 28-day oral exposure, the results of Ti content were significantly increased in the ileum in comparison with the control. The histopathological structure index of the ileum was significantly changed after TiO NPs exposure; villi height and crypt depth were decreased and increased, respectively. Meanwhile, TiO NPs treatment also significantly altered the transcription levels of genes. First, the GATA-3 and STAT-4 were upregulation and downregulation, respectively. Second, gene expressions of the Zonula Occludens-1, claudin (CLDN)-12, occludin, and myosin light chain kinase were significantly upregulated, while the CLDN-3 was decreased. Finally, the caspase-3, caspase-9, and caspase-12 were upregulated. The results of TUNEL staining indicated apoptosis in the ileum. In general, TiO NPs treatment significantly changed the intestine physical barrier in a dose-dependent manner. The toxicity of TiO NPs could be through the imbalance in the Th1/Th2.
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http://dx.doi.org/10.1002/tox.22934DOI Listing
September 2020

Effects of urbanization on productivity of terrestrial ecological systems based on linear fitting: a case study in Jiangsu, eastern China.

Sci Rep 2019 11 20;9(1):17140. Epub 2019 Nov 20.

School of Geography, Geomatics, and Planning, Jiangsu Normal University, Xuzhou, Jiangsu, 221116, China.

The terrestrial ecosystem productivity and foundation of regional ecosystem services have been significantly influenced by recent urbanization processes. This study assesses the changes in terrestrial ecosystem productivity in Jiangsu from the years of 2000 to 2015 in response to the urbanization. A linear model that incorporates the traditional equalization method is proposed to improve the estimation accuracy of net primary productivity (NPP) loss. Results revealed that the land area of urban construction expanded rapidly during the research period to encompass an area of 8672.8 km. The rate of expansion was highest during 2005-2010. Additionally, the expansion rate of urban construction land was considerably higher in southern Jiangsu compared to the northern areas. The NPP exhibited a rising tendency from the year of 2000 to 2015, and varied from 33.30 to 40.23 Tg C/y. It was higher in the central parts, which include the cities of Yancheng and Nantong. The increase in urban construction land has resulted in a significant reduction in the terrestrial ecosystem productivity, i.e. a cumulative NPP loss of 2.55-2.88 Tg C during the research period. The NPP losses due to the conversion from cropland to constrction land were the highest, followed by the wetland. The work in this paper indicates that the rate of future productivity losses in terrestrial ecosystem in northern Jiangsu would be faster than the southern areas.
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http://dx.doi.org/10.1038/s41598-019-53789-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6868215PMC
November 2019

Determination of the Total Phenolic Content in Wine Samples Using Potentiometric Method Based on Permanganate Ion as an Indicator.

Molecules 2019 Sep 9;24(18). Epub 2019 Sep 9.

School of Pharmacy, Binzhou Medical University, Yantai 264003, China.

A rapid and accurate determination method for total phenolic content is of great importance for controlling the quality of wine samples. A promising potentiometric detection approach, based on permanganate ion fluxes across ion-selective electrode membranes, is fabricated for measuring the total phenolic content of wine. The results show that the presence of phenols, such as gallic acid, leads to a potential increase for the potentiometric sensor. Additionally, the present sensor exhibits a linear potential response with the concentration range from 0.05 to 3.0 g/L with a detection limit of 6.6 mg/L calculated using gallic acid. These sensors also exhibit a fast response time, an acceptable reproducibility and long-term stability. These results indicate that the proposed potentiometric sensor can be a promising and reliable tool for the rapid determination of total phenolic content in wine samples.
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http://dx.doi.org/10.3390/molecules24183279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6766823PMC
September 2019

Detection of Phenylketonuria Markers Using a ZIF-67 Encapsulated PtPd Alloy Nanoparticle ([email protected])-Based Disposable Electrochemical Microsensor.

ACS Appl Mater Interfaces 2019 Jun 28;11(23):20734-20742. Epub 2019 May 28.

Materials Genome Institute , Shanghai University , Shanghai 200444 , China.

Phenylketonuria (PKU) is a common disease in congenital disorder of amino acid metabolism, which can lead to intellectual disability, seizures, behavioral problems, and mental disorders. We report herein a facile method to screen for PKU by the measurements of its metabolites (markers). In this work, a disposable electrochemical microsensor modified with a ZIF (zeolitic imidazolate framework)-based nanocomposite is constructed, in which ZIF-67 crystals are encapsulated with PtPd alloy nanoparticles (NPs) forming the nanocomposite ([email protected]). According to electrochemical measurements, the [email protected] microsensor shows good responses and selectivity to phenylpyruvic acid and phenylacetic acid, while almost no response toward other amino acid analogues is observed. Here, a new sensing mechanism based on the acylation reaction between the imidazole linker in ZIF-67 and carboxyl in PKU markers has been proposed and verified through the Fourier-transform infrared spectroscopy study. Moreover, the encapsulated PtPd NPs elevate the electron transfer capability of the [email protected] microsensor and further improve the electrochemical sensing performance. Finally, we demonstrate that the developed [email protected] microsensor has the possibility to sensing of PKU markers with high response and good specificity and may be extended to exploit the point-of-care rapid PKU screening.
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http://dx.doi.org/10.1021/acsami.9b05431DOI Listing
June 2019
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