Publications by authors named "Xinyu Wang"

677 Publications

Experimental Research on NO Viscosity and Absorption for (1-Ethyl-3-methylimidazolium Trifluoroacetate + Triethanolamine) Binary Mixtures.

Molecules 2021 Nov 18;26(22). Epub 2021 Nov 18.

Pollution Prevention Biotechnology Laboratory of Hebei Province, College of Environment Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China.

The viscosity (9.34-405.92 mPa·s) and absorption capacity (0.4394-1.0562 g·g) of (1-ethyl-3-methylidazolium trifluoroacetate + triethanolamine) binary blends atmospheric pressure in the temperature range of 303.15-343.15 K and at different mole fractions of [EMIM] [TFA] have been carried out. The molar fraction of [EMIM] [TFA] dependence of the viscosity and absorption capacity was demonstrated. The addition of a small amount of [EMIM] [TFA] into TEA led to rapidly decreased rates of binary blends' viscosity and absorption capacity. However, the viscosity and absorption of binary blends did not decrease significantly when [EMIM] [TFA] was increased to a specific value. Compared with the molar fraction of the solution, the temperature had no obvious effect on viscosity and absorption capacity. By modeling and optimizing the ratio of viscosity and absorption capacity of ([EMIM] [TFA] + TEA), it is proven that when the mole fraction of [EMIM] [TFA] is 0.58, ([EMIM] [TFA] + TEA) has the best viscosity and absorption capacity at the same time. In addition, at 303.15 K, ([EMIM] [TFA] + TEA) was absorbed and desorbed six times, the absorption slightly decreased, and the desorption increased.
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http://dx.doi.org/10.3390/molecules26226953DOI Listing
November 2021

CF101 alleviates OA progression and inhibits the inflammatory process via the AMP/ATP/AMPK/mTOR axis.

Bone 2021 Nov 23:116264. Epub 2021 Nov 23.

Northeast Agricultural University, Heilongjiang, Haerbin 150030, China; Laboratory of Heilongjiang Animal Disease Pathogenesis and Comparative Medicine, Heilongjiang, Haerbin 150030, China. Electronic address:

CF101 (IB-MECA) is an adenosine A3 receptor agonist that has anti-inflammatory and pain-relieving properties. Adenosine A3 receptor activation can delay the process of Osteoarthritis(OA) and prevent the occurrence of OA. However, the mechanism of CF101 on OA is still unknown. This study aimed to investigate the effect of CF101 on rats induced by anterior cruciate ligament-transection (ACLT) and rat chondrocytes induced by IL-1ß. ACLT-induced OA rats were administered CF101, and autophagy levels were measured to determine whether CD101 had an autophagy-mediated protective effect on articular cartilage. Furthermore, the mechanism by which CF101 protected articular cartilage in IL-1ß-induced chondrocytes mimicking OA was investigated. In rats treated with ACLT, CF101 was able to delay the progression of OA, as well as reduce inflammation and type II collagen degradation factors. In addition, in vitro experiments revealed that CF101 reduced type II collagen degradation factors in OA chondrocytes. In rats treated with ACLT and OA chondrocytes, CF101 enhanced autophagy and increased the ratio of AMP/ATP and AMPK protein levels while decreasing mTOR expression. Treatment of OA chondrocytes with 3-MA prior to treatment with CF101 resulted in inhibition of autophagy factor levels, as well as increased levels of inflammatory factors and type II collagen degradation compared to the CF101 group. These findings demonstrated that CF101 could protect articular cartilage against OA by enhancing the ratio of ATP/AMP and altering the AMPK/mTOR pathway to enhance autophagy and reduce inflammation. In addition, inhibition of autophagy resulted in a reduced CF101 effect.
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http://dx.doi.org/10.1016/j.bone.2021.116264DOI Listing
November 2021

Microfluidic Raman Sensing Using a Single Ring Negative Curvature Hollow Core Fiber.

Biosensors (Basel) 2021 Oct 30;11(11). Epub 2021 Oct 30.

Institute for Photonics and Advanced Sensing (IPAS), School of Physical Sciences, The University of Adelaide, Adelaide, SA 5005, Australia.

A compact microfluidic Raman detection system based on a single-ring negative-curvature hollow-core fiber is presented. The system can be used for in-line qualitative and quantitative analysis of biochemicals. Both efficient light coupling and continuous liquid injection into the hollow-core fiber were achieved by creating a small gap between a solid-core fiber and the hollow-core fiber, which were fixed within a low-cost ceramic ferrule. A coupling efficiency of over 50% from free-space excitation laser to the hollow core fiber was obtained through a 350 μm-long solid-core fiber. For proof-of-concept demonstration of bioprocessing monitoring, a series of ethanol and glucose aqueous solutions at different concentrations were used. The limit of detection achieved for the ethanol solutions with our system was ~0.04 vol.% (0.32 g/L). Such an all-fiber microfluidic device is robust, provides Raman measurements with high repeatability and reusability, and is particularly suitable for the in-line monitoring of bioprocesses.
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http://dx.doi.org/10.3390/bios11110430DOI Listing
October 2021

Construction of a Ferroptosis-Related Long Non-coding RNA Prognostic Signature and Competing Endogenous RNA Network in Lung Adenocarcinoma.

Front Cell Dev Biol 2021 8;9:751490. Epub 2021 Nov 8.

Department of Thoracic Surgery, Changhai Hospital, Navy Military Medical University, Shanghai, China.

Ferroptosis-related genes play an important role in the progression of lung adenocarcinoma (LUAD). However, the potential function of ferroptosis-related lncRNAs in LUAD has not been fully elucidated. Thus, to explore the potential role of ferroptosis-related lncRNAs in LUAD, the transcriptome RNA-seq data and corresponding clinical data of LUAD were downloaded from the TCGA dataset. Pearson correlation was used to mine ferroptosis-related lncRNAs. Differential expression and univariate Cox analysis were performed to screen prognosis related lncRNAs. A ferroptosis-related lncRNA prognostic signature (FLPS), which included six ferroptosis-related lncRNAs, was constructed by the least absolute shrinkage and selection operator (LASSO) Cox regression. Patients were divided into a high risk-score group and low risk-score group by the median risk score. Receiver operating characteristic (ROC) curves, principal component analysis (PCA), and univariate and multivariate Cox regression were performed to confirm the validity of FLPS. Enrichment analysis showed that the biological processes, pathways and markers associated with malignant tumors were more common in high-risk subgroups. There were significant differences in immune microenvironment and immune cells between high- and low-risk groups. Then, a nomogram was constructed. We further investigated the relationship between six ferroptosis-related lncRNAs and tumor microenvironment and tumor stemness. A competing endogenous RNA (ceRNA) network was established based on the six ferroptosis-related lncRNAs. Finally, we detected the expression levels of ferroptosis-related lncRNAs in clinical samples through quantitative real-time polymerase chain reaction assay (qRT-PCR). In conclusion, we identified the prognostic ferroptosis-related lncRNAs in LUAD and constructed a prognostic signature which provided a new strategy for the evaluation and prediction of prognosis in LUAD.
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http://dx.doi.org/10.3389/fcell.2021.751490DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606539PMC
November 2021

Naringenin: A Promising Therapeutic Agent against Organ Fibrosis.

Oxid Med Cell Longev 2021 11;2021:1210675. Epub 2021 Nov 11.

Department of Cardiology, The Affiliated Hospital of Southwest Medical University, Key Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease, Institute of Cardiovascular Research, Southwest Medical University, Luzhou, Sichuan 646000, China.

Fibrosis is the final common pathology of most chronic diseases as seen in the heart, liver, lung, kidney, and skin and contributes to nearly half of death in the developed countries. Fibrosis, or scarring, is mainly characterized by the transdifferentiation of fibroblasts into myofibroblasts and the excessive accumulation of extracellular matrix (ECM) secreted by myofibroblasts. Despite immense efforts made in the field of organ fibrosis over the past decades and considerable understanding of the occurrence and development of fibrosis gained, there is still lack of an effective treatment for fibrotic diseases. Therefore, identifying a new therapeutic strategy against organ fibrosis is an unmet clinical need. Naringenin, a flavonoid that occurs naturally in citrus fruits, has been found to confer a wide range of pharmacological effects including antioxidant, anti-inflammatory, and anticancer benefits and thus potentially exerting preventive and curative effects on numerous diseases. In addition, emerging evidence has revealed that naringenin can prevent the pathogenesis of fibrosis in vivo and in vitro via the regulation of various pathways that involved signaling molecules such as transforming growth factor-1/small mother against decapentaplegic protein 3 (TGF-1/Smad3), mitogen-activated protein kinase (MAPK), phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt), sirtuin1 (SIRT1), nuclear factor-kappa B (NF-B), or reactive oxygen species (ROS). Targeting these profibrotic pathways by naringenin could potentially become a novel therapeutic approach for the management of fibrotic disorders. In this review, we present a comprehensive summary of the antifibrotic roles of naringenin in vivo and in vitro and their underlying mechanisms of action. As a food derived compound, naringenin may serve as a promising drug candidate for the treatment of fibrotic disorders.
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http://dx.doi.org/10.1155/2021/1210675DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601819PMC
November 2021

Clinical characteristics of single human papillomavirus 53 infection: a retrospective study of 419 cases.

BMC Infect Dis 2021 Nov 15;21(1):1158. Epub 2021 Nov 15.

Women's Hospital, Zhejiang University School of Medicine, Hangzhou, 310006, People's Republic of China.

Background: Human papillomavirus (HPV) infection is the main cause of cervical cancer. Characteristics of HPV infections, including the HPV genotype and duration of infection, determine a patient's risk of high-grade lesions. Risk quantification of cervical lesions caused by different HPV genotypes is an important component of evaluation of cervical lesion. Data and evidence are necessary to gain a deeper understanding of the pathogenicity of different HPV genotypes. The present study investigated the clinical characteristics of patients infected with single human papillomavirus (HPV) 53.

Methods: This retrospective study analyzed the clinical data of patients who underwent cervical colposcopy guided biopsy between October 2015 and January 2021. The clinical outcomes and the follow-up results of the patients with single HPV53 infection were described.

Results: 82.3% of the initial histological results of all 419 patients with single HPV53 infection showed negative (Neg). The number of patients with cervical intraepithelial neoplasia (CIN)1, CIN2, CIN3, vaginal intraepithelial neoplasia (VaIN)1, CIN1 + VaIN1, CIN1 + VaIN2, and CIN2 + VaIN2 was 45, 10, 2, 9, 6, 1, and 1, respectively. Cancer was not detected in any patient. When the cytology was negative for intraepithelial lesion or malignancy (NILM), atypical squamous cells of undetermined significance (ASC-US) or low-grade squamous intraepithelial lesion (LSIL), we observed a significant difference in the distribution of histological results (P < 0.05). 95 patients underwent follow-up with cytology according to the exclusion criteria. No progression of high-grade lesions was observed during the follow-up period of 3-34 months.

Conclusions: The lesion caused by HPV53 infection progressed slowly. The pathogenicity of a single HPV53 infection was low.
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http://dx.doi.org/10.1186/s12879-021-06853-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8591956PMC
November 2021

The Value of MicroRNA-375 Detection for Triaging Primary Human Papillomavirus Positive Women: A Cross-Sectional Study in a General Population.

Front Oncol 2021 28;11:771053. Epub 2021 Oct 28.

Department of Gynecologic Oncology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Purpose: This study aims to validate the value of microRNA (miRNA) detection for triaging human papillomavirus (HPV)-positive women in the general population.

Patients And Methods: miR-375 detection in cervical exfoliated cells has been demonstrated to have the superior value to cytology in triaging primary HPV-positive women in the hospital population. In this study, residual samples of cervical exfoliated cells from 10,951 women in a general population were used to detect miRNA. The performance efficiency of miRNA detection in identifying high-grade cervical intraepithelial neoplasia (CIN) was evaluated. Pearson chi-square test and McNemar pairing test were used to compare miRNA detection and cytology.

Results: In valid 9,972 women aged 25-65, miR-375 expression showed a downward trend along with an increase in cervical lesion severity. The expression level of miR-375 ≤1.0 × 10 was identified as positive. In the HPV-positive and 12 HPV genotypes other than 16/18 (HR12)-positive women, miR-375 detection showed equivalent sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) to that of cytology (≥ASC-US) and higher or similar sensitivity and NPV but lower specificity and PPV than that of cytology (≥ASC-H) in identifying CIN3+ and CIN2+. In HPV 16-positive women, miR-375 positivity had higher sensitivity and NPV but lower specificity and PPV than that of cytology (≥ASC-H and HSIL) in identifying CIN3+ and CIN2+. The immediate CIN3+ risk of miR-375 positivity was 19.8% (61/308) in HPV-positive, 10.8% (22/204) in HR12-positive, and 43.5% (37/85) in HPV16-positive women, respectively.

Conclusion: The detection of miR-375 in cervical exfoliated cells may be an optional method for triaging primary HPV-positive women in population-based cervical cancer screening.
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http://dx.doi.org/10.3389/fonc.2021.771053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581639PMC
October 2021

Re-exploring α-Cyano-4-Hydroxycinnamic Acid as a Reactive Matrix for Selective Detection of Glutathione via MALDI-MS.

J Am Soc Mass Spectrom 2021 Nov 11. Epub 2021 Nov 11.

State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China.

Herein, we re-explored α-cyano-4-hydroxycinnamic acid (CHCA) as a reactive matrix for selective and sensitive analysis of glutathione (GSH) by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). CHCA efficiently reacted with GSH, and the resulting CHCA-GSH conjugate was readily detected by MALDI-MS without interferences. The detection limit of the CHCA-GSH conjugate decreased to 200 pmol μL, which was 2 orders of magnitude lower than that of pure GSH.Forapplication, CHCA was successfully applied for the detection of GSH, present in HepG2 cell lysates. The results demonstrated detection advantages of simple, high-throughput, and selective and screening of GSH in biological samples by MALDI-MS.
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http://dx.doi.org/10.1021/jasms.1c00261DOI Listing
November 2021

De-escalation antibiotic therapy alleviates organ injury through modulation of NETs formation during sepsis.

Cell Death Discov 2021 Nov 10;7(1):345. Epub 2021 Nov 10.

Division of Trauma and Surgical Intensive Care Unit, Affiliated Jinling Hospital, Medical School of Nanjing University, Nanjing, 210002, Jiangsu Province, PR China.

Empiric broad-spectrum antimicrobials therapy is suggested to be started immediately for sepsis patients. Empiric antimicrobial therapy should be narrowed once pathogen identification and sensitivities are established. However, the detailed mechanisms of de-escalation strategy are still unclear. Here we hypothesized neutrophil extracellular traps (NETs) played an essential role and de-escalation strategy might alleviate organs injury through regulation of NETs formation in sepsis. We evaluated the effect of imipenem and ceftriaxone on NETs formation in vitro and examined the role of reactive oxygen species (ROS). Next, we designed de-escalation and escalation strategy in cecum ligation and puncture (CLP) models. Organ injury, inflammatory cytokines, NETs levels were compared and evaluated. In CLP models, de-escalation therapy resulted in an increased serum MPO-DNA level during the early stage and decreased MPO-DNA level during late stage, which exerted the reverse effects in escalation therapy. Inflammatory response and organ injury exacerbated when eliminated NETs with DNAse I during the early stage of sepsis (p < 0.01). Histopathological analysis showed decreased injury in lung, liver, and intestine in de-escalation therapy compared with escalation therapy (p < 0.01). De-escalation therapy results in the highest 6-day survival rate compared with the control group (p < 0.01), however, no significant difference was found between de-escalation and escalation group (p = 0.051). The in vitro study showed that the imipenem could promote, while the ceftriaxone could inhibit the formation of NETs in PMA-activated PMNs through a ROS-dependent manner. We firstly demonstrate that de-escalation, not escalation, therapy reduces organ injury, decreases inflammatory response by promoting NETs formation in the early stage, and inhibiting NETs formation in the late stage of sepsis.
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http://dx.doi.org/10.1038/s41420-021-00745-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8580974PMC
November 2021

The fabrication of graphitic carbon nitride hollow nanocages with semi-metal 1T' phase molybdenum disulfide as co-catalysts for excellent photocatalytic nitrogen fixation.

J Colloid Interface Sci 2021 Oct 28;608(Pt 2):1229-1237. Epub 2021 Oct 28.

School of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao 266590, China. Electronic address:

Improving the efficiency of photogenerated carrier separation is essential for photocatalytic N fixation. Herein, the 2D semi-metal 1T'-MoS was uniformly distributed in g-CN nanocages (CNNCs) by a hydrothermal method, and the 1T'-MoS/CNNC composite was obtained. 1T'-MoS as a co-catalyst can promote the transfer of electrons, improve the separation efficiency of photogenerated carriers, and also increase the number of effective active sites. In addition, the unique nanocage morphology of CNNCs is conducive to the scattering and reflection of incident light and improves the light absorption capacity. Therefore, the optimized 1T'-MoS/CNNC composite (5 wt%) shows a significantly improved photocatalytic N fixation rate (9.8 mmol L h g) and good stability, which is significantly higher than pure CNNCs (2.9 mmol L h g), Pt/CNNC (8.2 mmol L h g) and Pt/g-CN nanosheet (CNNS, 6.3 mmol L h g). This work guides guidance for the design of green and efficient N fixation photocatalysts.
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http://dx.doi.org/10.1016/j.jcis.2021.10.153DOI Listing
October 2021

Development and validation of a non-invasive model for diagnosing HBV-related liver cirrhosis.

Clin Chim Acta 2021 Nov 5;523:525-531. Epub 2021 Nov 5.

Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Fudan University, Shanghai 200032, China. Electronic address:

Background: Liver cirrhosis is closely related to the abnormal liver function and occurrence of liver cancer. Accurate non-invasive assessment of liver cirrhosis is of great significance for preventing disease progression and treatment decision-making. We aim to develop and validate a non-invasive diagnostic model for liver cirrhosis in patients with chronic hepatitis B (CHB).

Methods: From July 2015 to April 2017, seven-hundred fifty-four patients with primary HBV-related liver cancer who underwent hepatectomy were reprospectively recruited. All patients were examined with 2D-SWE and serologic testing preoperatively, which were utilized for measurement of liver stiffness and serum fibrosis models. The stage of liver fibrosis was evaluated using a resected liver specimen. Least absolute shrinkage and selection operator (Lasso) regression was used for feature selection and binary logistic regression analysis was chosen to build a diagnostic model, which was presented as a nomogram and evaluated for calibration, discrimination and clinical usefulness. The performance of noninvasive model was then prospectively validated in an independent cohort (361 patients) by the ROC curve analysis.

Results: The diagnostic model, which consists of 5 selected clinical characteristics (PIII-NP, IV-C, Hyaluronan, Platelet and Liver stiffness), showed the strongest correlation with liver fibrosis stage (ρ = 0.702, P < 0.05). Compared with APRI, FIB-4, King's Score, and Forns Index, the model presented the optimal discrimination and the best predictive performance with the highest AUC in the training cohort (0.866, 95%CI 0.840-0.892, P < 0.05) and validation cohorts (0.852, 95%CI 0.813-0.890, P < 0.05). Decision curve analysis demonstrated that nomogram based on the model was extremely useful for diagnosing cirrhosis in patients with chronic hepatitis B.

Conclusion: This study proposes a non-invasive diagnostic model that incorporates the clinical predictors which can be conveniently used in the individualized diagnosis of HBV-related liver cirrhosis.
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http://dx.doi.org/10.1016/j.cca.2021.11.004DOI Listing
November 2021

The clinical practice experience in psychiatric clinic of nursing students and career intention in China: A qualitative study.

J Prof Nurs 2021 Sep-Oct;37(5):916-922. Epub 2021 Jul 17.

School of Health Sciences, Wuhan University, Wuhan, Hubei, China. Electronic address:

Background: Nursing students face a lot of complicated situations and problems when they participate in psychiatric clinical practice for the first time. However, the nursing students' internship experience and career intention in psychiatry have not been well described in China.

Purpose: This study aims to explore the experience and career intention of Chinese nursing undergraduates during their first psychiatric internship.

Method: This is a qualitative design using phenomenological approach. Data were collected through a case-study, semi-structured, two face-to-face in-depth interviews (N = 13) with third-year nursing students from a university in China on their first clinical internship in a psychiatric department. We followed the consolidated criteria for reporting qualitative research (COREQ) checklist.

Results: Four themes were identified: negative stereotype perceptions about mental illness, professional maturation, personal development, attitude towards psychiatric patients and career choice.

Conclusions: The experience of nursing students in psychiatric clinical practice was constantly changing. This study indicated that nursing students went through a continuous development process of psychiatric care professional maturation and personal development. However, psychiatric nursing was not an ideal career choice of nursing students. This study provides guidance for educators to optimize the internship experience and take comprehensive measures to improve the professional attractiveness and professional identity of psychiatric nursing.
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http://dx.doi.org/10.1016/j.profnurs.2021.07.013DOI Listing
November 2021

Structural and PK-guided identification of indole-based non-acidic autotaxin (ATX) inhibitors exhibiting high in vivo anti-fibrosis efficacy in rodent model.

Eur J Med Chem 2021 Oct 30;227:113951. Epub 2021 Oct 30.

Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang, 110016, PR China. Electronic address:

In recent decades, pharmacological targeting of the autotaxin (ATX)/lysophosphatidic acid (LPA) axis accounted for excellent disease management benefits. Herein, to extend the scope of structure-activity relationships (SARs), fifteen indole-based carbamate derivatives (1-15) were prepared to evaluate the ATX inhibitory potency. Among them, compound 4 bearing morpholine moiety was identified as the optimal ATX inhibitor (0.41 nM), superior to the positive control GLPG1690 (2.90 nM). To resolve the intractable issue of poor pharmacokinetic (PK) property, urea moiety was introduced as a surrogate of carbamate which furnished compounds 16-30. The dedicated modification identified the diethanolamine entity 30 with satisfactory water solubility and PK profiles with a minimum sacrifice of ATX inhibition (2.17 nM). The most promising candidate 30 was evaluated for anti-fibrosis effect in a bleomycin challenged mice lung fibrosis model. Upon treatment with 30, the in vivo ATX activity in both lung homogenate and broncheoalveolar fluid (BALF) sample was significantly down-regulated. Furthermore, the gene expression of pro-fibrotic cytokines transforming growth factor-β (TGF-β), interleukin- 6 (IL-6) and tumor necrosis factor-α (TNF-α) in lung tissue was reduced to normal level. Collectively, the promising biological effects may advocate potential application of 30 in fibrosis relevant diseases.
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http://dx.doi.org/10.1016/j.ejmech.2021.113951DOI Listing
October 2021

Predicting the effective thermal conductivity of unfrozen soils with various water contents based on artificial neural network.

Nanotechnology 2021 Nov 19;33(6). Epub 2021 Nov 19.

Institute of Thermal Science and Technology, Shandong University, Jinan 250061, People's Republic of China.

The effective thermal conductivity of soils is a crucial parameter for many applications such as geothermal engineering, environmental science, and agriculture and engineering. However, it is pretty challenging to accurately determine it due to soils' complex structure and components. In the present study, the influences of different parameters, including silt content (), sand content (), clay content (), quartz content (), porosity, and water content on the effective thermal conductivity of soils, were firstly analyzed by the Pearson correlation coefficient. Then different artificial neural network (ANN) models were developed based on the 465 groups of thermal conductivity of unfrozen soils collected from the literature to predict the effective thermal conductivity of soils. Results reveal that the parameters of,,, andhave a relatively slight influence on the effective thermal conductivity of soils compared to the water content and porosity. Although the ANN model with six parameters has the highest accuracy, the ANN model with two input parameters (porosity and water content) could predict the effective thermal conductivity well with acceptable accuracy and= 0.940. Finally, a correlation of the effective thermal conductivity for different soils was proposed based on the large number of results predicted by the two input parameters ANN-based model. This correlation has proved to have a higher accuracy without assumptions and uncertain parameters when compared to several commonly used existing models.
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http://dx.doi.org/10.1088/1361-6528/ac3688DOI Listing
November 2021

Neutrophil extracellular trap formation index predicts occurrences of deep surgical site infection after laparotomy.

Ann Transl Med 2021 Sep;9(17):1373

Division of Trauma and Surgical Intensive Care Unit, Research Institute of General Surgery, Affiliated Jinling Hospital, Medical School of Nanjing University, Nanjing, China.

Background: Deep surgical site infections (DSSIs) are serious complications after laparotomy. Neutrophil extracellular traps (NETs) play a vital role in the development of DSSI. Here, we focused on a new approach to predicting the occurrence of DSSI through the detection of the NET formation index (NFI), and compared its prediction ability with other clinical infection indicators.

Methods: Patients who received laparotomy were prospectively enrolled in this study. General information, APACHE II score, SOFA score, and serum infection indicators were recorded. The postoperative abdominal drainage fluid was collected within 3 days after the operation for quantification of the NFI.

Results: A total of 92 consecutive patients were included, with 22 patients were diagnosed with DSSI. The NFI in the DSSI group was 32.70%±19.33% while the corresponding index was 10.70%±8.25% in the non-DSSI group (P<0.01). The mean APACHE II and SOFA score had significant differences between the two groups. The NFI was positively correlated with the APACHE II score (P<0.01, r=0.269) and SOFA score (P=0.013, r=0.258). Patients with a high NFI (NFI >13.86%) had a higher risk of developing DSSI. According to the receiver operating characteristic (ROC) curve, the area under the ROC curve (AUC) of the NFI, C-reactive protein (CRP) and procalcitonin (PCT) were 0.912, 0.748 and 0.731, respectively.

Conclusions: In this cohort of surgical patients, the quantification of the NFI had a considerable predictive value for early identification of DSSI. The NFI in drainage fluid turned out to be a more sensitive and specific predictor of DSSI than serum infection indicators including CRP and PCT.
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http://dx.doi.org/10.21037/atm-21-1078DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8506538PMC
September 2021

Gut Microbiota-Derived Metabolites in Colorectal Cancer: The Bad and the Challenges.

Front Oncol 2021 18;11:739648. Epub 2021 Oct 18.

Department of Gastroenterology and Hepatology, General Hospital, Tianjin Medical University, Tianjin Institute of Digestive Diseases, Tianjin Key Laboratory of Digestive Diseases, Tianjin, China.

Accumulating evidence from studies in humans and animal models has elucidated that gut microbiota, acting as a complex ecosystem, contributes critically to colorectal cancer (CRC). The potential mechanisms often reported emphasize the vital role of carcinogenic activities of specific pathogens, but in fact, a series of metabolites produced from exogenous dietary substrates or endogenous host compounds occupy a decisive position similarly. Detrimental gut microbiota-derived metabolites such as trimethylamine-N-oxide, secondary bile acids, hydrogen sulfide and N-nitroso compounds could reconstruct the ecological composition and metabolic activity of intestinal microorganisms and formulate a microenvironment that opens susceptibility to carcinogenic stimuli. They are implicated in the occurrence, progression and metastasis of CRC through different mechanisms, including inducing inflammation and DNA damage, activating tumorigenic signaling pathways and regulating tumor immunity. In this review, we mainly summarized the intimate relationship between detrimental gut microbiota-derived metabolites and CRC, and updated the current knowledge about detrimental metabolites in CRC pathogenesis. Then, multiple interventions targeting these metabolites for CRC management were critically reviewed, including diet modulation, probiotics/prebiotics, fecal microbiota transplantation, as well as more precise measures such as engineered bacteria, phage therapy and chemopreventive drugs. A better understanding of the interplay between detrimental microbial metabolites and CRC would hold great promise against CRC.
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http://dx.doi.org/10.3389/fonc.2021.739648DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8558397PMC
October 2021

Aqueous-Natural Deep Eutectic Solvent-Enhanced 5-Hydroxymethylfurfural Production from Glucose, Starch, and Food Wastes.

ChemSusChem 2021 Nov 3. Epub 2021 Nov 3.

College of Energy, Xiamen University, Xiamen, 361102, P. R. China.

5-Hydroxymethylfurfural (HMF) has been regarded as an essential building block for synthesizing chemicals and biofuels, but the direct conversion of biomass to HMF is still a critical challenge. In this study, a cheap and green aqueous-natural deep eutectic solvent (A-NADES) was used to efficiently produce HMF from various carbohydrates, with a low amount of SnCl as the catalyst. High HMF yields of 64.3, 64.0, 61.3, and 54.5 % were obtained from glucose, starch, rice waste, and bread waste at 130 °C in the A-NADES/MIBK (methyl isobutyl ketone) biphasic system, respectively. Mechanistic study results revealed that the water in A-NADES was the key factor in facilitating the conversion of Sn atom existent forms and promoted the HMF production. The choline chloride in NADES stabilized the HMF product with the cooperation of extraction solvent MIBK and inhibited the side reactions of HMF. This study investigated the multiple interaction functions of A-NADES to feedstocks and proposed a practical application of novel solvents to facilitate biomass and food waste conversion with a green method.
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http://dx.doi.org/10.1002/cssc.202101889DOI Listing
November 2021

PPARγ/LXRα axis mediated phenotypic plasticity of lung fibroblasts in silica-induced experimental silicosis.

Environ Pollut 2022 Jan 28;292(Pt A):118272. Epub 2021 Oct 28.

School of Public Health, Zhengzhou University, Henan, China. Electronic address:

Silicosis is a disease mainly caused by pulmonary interstitial fibrosis caused by long-term inhalation of dust with excessively high content of free SiO. Transdifferentiation of lung fibroblasts into myofibroblasts is an important cellular basis for silicosis, but the key transcription factors (TFs) involved in this process are still unclear. In order to explore the biological regulation of transcription factor PPARγ/LXRα in silica-induced pulmonary fibrosis, this study explored the molecular mechanism of PPARγ/LXRα involved in regulating transcription factors related to SiO-induced lung injury at the cellular level and in animal models. ChIP-qPCR detected that PPARγ directly regulated the transcriptional activity of the LXRα gene promoter, while the PPARγ agonist RSG increased the expression of LXRα. In addition, we demonstrated in the cell model that upregulation of LXRα can inhibit silica-mediated fibroblast transdifferentiation, accompanied by an increase in the expression of SREBF1, PLTP and ABCA1. The results of LXRα silencing experiment matched those of overexpression experiment. These studies explored the role of LXRα in plasticity and phenotypic transformation between lung fibroblasts and myofibroblasts. Therefore, inhibiting or reversing the transdifferentiation of lung fibroblasts to myofibroblasts by intervening PPARγ/LXRα may provide a new therapeutic target for the treatment of silicosis.
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http://dx.doi.org/10.1016/j.envpol.2021.118272DOI Listing
January 2022

Protective effects of low-magnitude high-frequency vibration on high glucose-induced osteoblast dysfunction and bone loss in diabetic rats.

J Orthop Surg Res 2021 Oct 30;16(1):650. Epub 2021 Oct 30.

Department of Orthopaedic Trauma, The First Hospital of Jilin University, Changchun, Jilin, China.

Objective: Low-magnitude high-frequency vibration (LMHFV) has been reported to be capable of promoting osteoblast proliferation and differentiation. Reduced osteoblast activity and impaired bone formation were related to diabetic bone loss. We investigated the potential protective effects of LMHFV on high-glucose (HG)-induced osteoblasts in this study. In addition, the assessment of LMHFV treatment for bone loss attributed to diabetes was also performed in vivo.

Method: MC3T3-E1 cells induced by HG only or treated with LMHFV were treated in vitro. The experiments performed in this study included the detection of cell proliferation, migration and differentiation, as well as protein expression. Diabetic bone loss induced by streptozotocin (STZ) in rats was established. Combined with bone morphometric, microstructure, biomechanical properties and matrix composition tests, the potential of LMHFV in treating diabetes bone loss was explored.

Results: After the application of LMHFV, the inhibiting effects of HG on the proliferation, migration and differentiation of osteoblasts were alleviated. The GSK3β/β-catenin pathway was involved in the protective effect of LMHFV. Impaired microstructure and biomechanical properties attributed to diabetes were ameliorated by LMHFV treatment. The improvement of femur biomechanical properties might be associated with the alteration of the matrix composition by the LMHFV.

Conclusion: LMHFV exhibited a protective effect on osteoblasts against HG by regulating the proliferation, migration and differentiation of osteoblasts. The function of promoting bone formation and reinforcing bone strength made it possible for LMHFV to alleviate diabetic bone loss.
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http://dx.doi.org/10.1186/s13018-021-02803-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8557505PMC
October 2021

Near-Equilibrium Growth of Chemically Stable Covalent Organic Framework/Graphene Oxide Hybrid Materials for the Hydrogen Evolution Reaction.

Angew Chem Int Ed Engl 2021 Oct 26. Epub 2021 Oct 26.

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.

Facile synthesis and post-processing of covalent organic frameworks (COFs) under mild synthetic conditions are highly sought after and important for widespread utilizations in catalysis and energy storage. Here we report the synthesis of the chemically stable aza-fused COFs BPT-COF and PT-COF by a liquid-phase method. The process involves the spontaneous polycondensation of vicinal diamines and vicinal diketones, and is driven by the near-equilibrium growth of COF domains at a very low monomer concentration. The method permits in situ assembly of COFs and COF-GO hybrid materials and leads to the formation of a uniform conducting film on arbitrary substrates on vacuum filtration. When used as electrocatalysts, the as-prepared membranes show a fast hydrogen evolution reaction (HER) with a low overpotential (45 mV at 10 mA cm ) and a small Tafel slope (53 mV dec ), which are the best among metal-free catalysts. Our results may open a new route towards the preparation of highly π-conjugated COFs for multifunctional applications.
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http://dx.doi.org/10.1002/anie.202113067DOI Listing
October 2021

Strontium-Loaded Nanotubes of Ti-24Nb-4Zr-8Sn Alloys for Biomedical Implantation.

J Biomed Nanotechnol 2021 Sep;17(9):1812-1823

Central Laboratory, Peking University School and Hospital of Stomatology, Beijing, 100081, China.

Ti-24Nb-4Zr-8Sn (Ti2448) alloys, with a relatively low elastic modulus and unique mechanical properties, are desirable materials for oral implantation. In the current study, a multifaceted strontium-incorporating nanotube coating was fabricated on a Ti2448 alloy (Ti2-NTSr) through anodization and hydrothermal procedures. the Ti2-NTSr specimens demonstrated better osteogenic properties and more favorable osteoimmunomodulatory abilities. Moreover, macrophages on Ti2-NTSr specimens could improve the recruitment and osteogenic differentiation of osteoblasts. dense clots with highly branched, thin fibrins and small pores existed on the Ti2-NTSr implant in the early stage after surgery. Analysis of the deposition of Ca and P elements, hard tissue slices and the bone-implant contact rate (BIC%) of the Ti2-NTSr implants also showed superior osseointegration. Taken together, these results demonstrate that the Ti2-NTSr coating may maximize the clinical outcomes of Ti2448 alloys for implantation applications.
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http://dx.doi.org/10.1166/jbn.2021.3160DOI Listing
September 2021

Enhanced Photocatalytic Antibacterial Properties of TiO Nanospheres with Rutile/Anatase Heterophase Junctions and the Archival Paper Protection Application.

Nanomaterials (Basel) 2021 Sep 30;11(10). Epub 2021 Sep 30.

School of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao 266590, China.

TiO has been generally studied for photocatalytic sterilization, but its antibacterial activities are limited. Herein, TiO nanospheres with rutile/anatase heterophase junctions are prepared by a wet chemical/annealing method. The large BET surface area and pore size are beneficial for the absorption of bacteria. The rutile/anatase heterojunctions narrow the bandgap, which enhances light absorption. The rutile/anatase heterojunctions also efficiently promote the photogenerated carriers' separation, finally producing a high yield of radical oxygen species, such as •O and •OH, to sterilize bacteria. As a consequence, the obtained TiO nanospheres with rutile/anatase heterojunctions present an improved antibacterial performance against (98%) within 3 h of simulated solar light irradiation, exceeding that of TiO nanospheres without annealing (amorphous) and TiO nanospheres annealing at 350 and 550 °C (pure anatase). Furthermore, we design a photocatalytic antibacterial spray to protect the file paper. Our study reveals that the TiO nanospheres with rutile/anatase heterojunctions are a potential candidate for maintaining the durability of paper in the process of archival protection.
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http://dx.doi.org/10.3390/nano11102585DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539383PMC
September 2021

Activation of O by zero-valent zinc assisted with Cu(II) for organics removal: Performance and mechanism.

J Hazard Mater 2021 Oct 14;424(Pt B):127506. Epub 2021 Oct 14.

College of Architecture & Environment, Sichuan University, Chengdu 610065, China; State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610065, China. Electronic address:

This study proposes a method to activate O by accelerating the corrosion process for zero-valent zinc (ZVZ) with the assistance of Cu(II), promoting the consecutive production of reactive oxygen species. The mechanisms for reactive oxygen species generation are clarified with metronidazole (MTZ) as the targeted pollutant. The outcome suggests the association of Cu(Ⅱ) and ZVZ presents an apparent cooperative activity, an enhancement of 85% in MTZ removal is attained for the ZVZ/Cu(Ⅱ) system after 10 min compared to that for ZVZ. Analysis of the mechanisms involved indicates that this improvement is due to the addition of Cu(Ⅱ), which can accelerate the corrosion of ZVZ. In addition, quenching experiments and electron paramagnetic resonance (EPR) technology show that superoxide radicals (·O) result in rapid MTZ degradation. The primary component that is liable for O activation and a certain amount of HO generation is verified to be ZVZ. Moreover, Cu(I) is detected in the ZVZ/Cu(Ⅱ) system, which arises from a direct reduction pathway driven by ZVZ and an indirect reduction pathway driven by active hydrogen atoms.
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http://dx.doi.org/10.1016/j.jhazmat.2021.127506DOI Listing
October 2021

Research progress, models and simulation of electrospinning technology: a review.

J Mater Sci 2021 Oct 13:1-47. Epub 2021 Oct 13.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, 430070 People's Republic of China.

In recent years, nanomaterials have aroused extensive research interest in the world's material science community. Electrospinning has the advantages of wide range of available raw materials, simple process, small fiber diameter and high porosity. Electrospinning as a nanomaterial preparation technology with obvious advantages has been studied, such as its influencing parameters, physical models and computer simulation. In this review, the influencing parameters, simulation and models of electrospinning technology are summarized. In addition, the progresses in applications of the technology in biomedicine, energy and catalysis are reported. This technology has many applications in many fields, such as electrospun polymers in various aspects of biomedical engineering. The latest achievements in recent years are summarized, and the existing problems and development trends are analyzed and discussed.
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http://dx.doi.org/10.1007/s10853-021-06575-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8513391PMC
October 2021

How Much Are People Willing to Pay for Clean Air? Analyzing Housing Prices in Response to the Smog Free Tower in Xi'an.

Int J Environ Res Public Health 2021 09 28;18(19). Epub 2021 Sep 28.

School of Economics and Management, China University of Mining and Technology, Xuzhou 221116, China.

The Smog Free Tower (SFT) in the city of Xi'an, China, is the world's first outdoor architecture that uses solar energy and filtration technology to purify polluted air. It provides a unique opportunity to explore residents' willingness to pay for air quality and their related behaviors. Drawing on data collected after the establishment of the SFT, this paper reveals the characteristics of changes in people's willingness to pay for clean air. We found that, prior to the release of an assessment report on the SFT, housing prices had an inverted U-shaped relationship with the distance to the SFT, which indicated people tended to purchase houses a certain distance away from the SFT. The threshold value of distance was inversely related to the greening ratio of the residential area. However, after the publication of the experimental report on the SFT, housing prices decreased as the distance to the SFT increased, indicating the closer the house was to the SFT, the more likely people were to buy it. These changes confirmed that people are willing to pay for clean air. The convenience of transportation had a significant moderating effect on the willingness to pay for clean air, however. In other words, people may buy houses with lower air quality if they have better transportation accessibility. The findings of this paper may have practical implications for environmental governance, urban planning, residential satisfaction, and real estate market regulation.
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http://dx.doi.org/10.3390/ijerph181910210DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8508453PMC
September 2021

A cation exchange strategy to construct Rod-shell CdS/CuS nanostructures for broad spectrum photocatalytic hydrogen production.

J Colloid Interface Sci 2021 Oct 1;608(Pt 1):158-163. Epub 2021 Oct 1.

School of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao 266590, China. Electronic address:

Herein, CuS as the outer shell is grown on CdS nanorods (NRs) to construct rod-shell nanostructures (CdS/CuS) by a rapid, scalable and facile cation exchange reaction. The CdS NRs are firstly synthesized by a hydrothermal route, in which thiourea as the precursor of sulfur and ethylenediamine (EDA) as the solvent. And then, the outer shells of CdS NRs are successfully exchanged by CuS via a cation exchange reaction. The obtained CdS/CuS rod-shell NRs exhibit much enhanced activity of hydrogen production (640.95 μmol h g) in comparison with pure CdS NRs (74.1 μmol h g) and pure CuS NRs (0 μmol h g). The enhanced photocatalytic activity of CdS/CuS rod-shell NRs owns to the following points: i) the photogenerated electrons generated by CdS quickly migrate to CuS without any barrier due to rod-shell structure by the in-situ cation exchange reaction, a decreased carrier recombination is achieved; ii) CuS as outer shells broaden the light absorption range of CdS/CuS rod-shell NRs into visible or even NIR light, which can produce more electrons and holes. This work inspires people to further study the rod-shell structured photocatalyst through the cation exchange strategy to further solar energy conversion.
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http://dx.doi.org/10.1016/j.jcis.2021.09.190DOI Listing
October 2021

Multiplexed Analysis of Photochemical Oxidants Using a Nanoparticle-Based Optoelectronic Nose.

Anal Chem 2021 10 6;93(41):13990-13997. Epub 2021 Oct 6.

Ecological Business Department-Automobile Materials Research Division, Automotive Data of China Co., Ltd., No. 68 East Xianfeng Road, Tianjin 300300, P. R. China.

Photochemical pollutants pose a substantial threat to human health in both outdoor and indoor environments. Herein, we prepare a class of gold nanoparticle-based colorimetric sensor arrays on optimized hydrophobic substrates using a simple pin-printing method for accurate identification and quantification of various gas-phase oxidants, as these microdetectors are low cost, sensitive, and easy to fabricate. For an array of AuNP sensors modified with various thiol-terminated ligands, a unique and distinguishable change in color (i.e., red, green, and blue response patterns) was obtained for each specific pollutant for molecular fingerprinting. Remarkable discrimination among 15 gases at a fairly low vapor concentration (i.e., 500 ppb) was illustrated using standard chemometric methods. Using digital imaging, the AuNP colorimetric sensor array offers ultrasensitive dosimetric identification of gas-phase oxidants relevant to outdoor and indoor air pollution, with limits of detection generally at sub-ppb levels for 2 h measurement. As a practical application, the sensor array is able to predict the overall air quality in indoor office environments over 24 h. Such sensor array based on chemically induced sintering of nanoparticles has significant implications for the development of nanosensors used in continuous monitoring of potential airborne pollutants at low concentrations from a large number of locations in a cost-effective manner.
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http://dx.doi.org/10.1021/acs.analchem.1c03457DOI Listing
October 2021

Demonstration of an external cavity semiconductor mode-locked laser.

Opt Lett 2021 Oct;46(19):4855-4858

Electrically pumped semiconductor mode-locked lasers (SMLs) are promising in a wide range of applications due to compact size, high energy efficiency, and low cost. However, the long gain interaction length increases the spontaneous emission noise. In this Letter, an external cavity structure is adopted to improve the SML noise performance, as well as the flexibility to adjust the repetition rate. Two external cavity SMLs with repetition rates of 255 MHz and 10 GHz are demonstrated. For the 10 GHz SML, the signal-noise-ratio and radio frequency linewidth of the fundamental frequency reach 81.1 dB and 40 Hz, respectively. The high performance makes the laser a promising light source for microwave and communication applications.
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http://dx.doi.org/10.1364/OL.428794DOI Listing
October 2021

Quantitative radio-thin-layer chromatography and positron emission tomography studies for measuring streptavidin transduced chimeric antigen receptor T cells.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Oct 23;1182:122944. Epub 2021 Sep 23.

Molecular Imaging Center, NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063, China.

The proliferation of chimeric antigen receptor (CAR) T cells is closely related to their efficacy, but it is still a great challenge to monitor and quantify CAR T cells in vivo. Based on the high affinity (Kd ≈ 10 M) of streptavidin (SA) and biotin, radiolabeled biotin may be used to quantify SA-transduced CAR T cells (SA-CAR T cells). Radio-thin-layer chromatography (radio-TLC) and positron emission tomography (PET) are highly sensitive for trace analysis. Our aim was to develop radio-TLC and PET methods to quantify SA-CAR T cells in vitro and in vivo. First, we developed [Ga]-DOTA-biotin. Commercially available SA was used as a standard, and quantitative standard curves were established in vitro and in vivo by radio-TLC and PET. Furthermore, the feasibility of the method was verified in Raji model mice. The linear range of radio-TLC was 0.02 ∼ 0.15 pmol/μL with R = 0.9993 in vitro. The linear range of PET was 0.02 ∼ 0.76 pmol/μL with R = 0.9986 in vivo. SA in CAR T cells can also be accurately quantified in a Raji leukemia model according to PET imaging. The radio-TLC/PET method established in this study is promising for using in the dynamic monitoring and analysis of SA-CAR T cells during therapy.
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http://dx.doi.org/10.1016/j.jchromb.2021.122944DOI Listing
October 2021

A novel mitochondrial-targeting fluorescent probe based on 1,4-dihydropyridine to visualize and monitor the viscosity of live cells and mice .

Anal Methods 2021 09 30;13(37):4238-4245. Epub 2021 Sep 30.

College of Chemistry, Jilin University, Changchun 130021, Jilin, China.

Cell viscosity is related to some diseases, such as diabetes, atherosclerosis, and Alzheimer's disease. These diseases can cause abnormal viscosity of the cell mitochondrial matrix. 1,4-Dihydropyridine (DHP) is an important organic compound with biological activity and is widely used in drug research. However, there are few studies on its optical properties, especially in the design of viscous fluorescent probes. In this study, a fluorescent probe for viscosity detection using 1,4-dihydropyridine as the fluorophore and indole iodide salt as the recognition group was designed and synthesized. The probe has the advantages of a deep-red emission, low cytotoxicity, good biocompatibility and excellent anti-interference ability. In addition, the probe also has the ability to target mitochondria and has been successfully applied to the detection of the viscosity response of HeLa cells and living mice, and has good clinical application potential.
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http://dx.doi.org/10.1039/d1ay01206aDOI Listing
September 2021
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