Publications by authors named "Xin Jin"

1,589 Publications

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To explore the potential molecular mechanism of complex rose - bergamot essential oil on anti-anxiety with facial spots based on network pharmacology.

J Cosmet Dermatol 2022 Aug 10. Epub 2022 Aug 10.

College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo, China.

Objective: To explore the potential mechanism of complex rose-bergamot essential oil against anxiety accompanied with facial spots through network pharmacology.

Methods: The components of rose and bergamot essential oils were determined by GC-MS and obtained from literature, and their action targets were predicted by PubChem, SwissADME and SwissTargetPrediction databases. Using "Anxiety disorder" and "Facial pigmentation" as key words, the relevant targets of anxiety disorder and facial spots were mined in the databases of DrugBank, TTD, GeneCards and OMIM. The intersection targets of complex rose-bergamot essential oil and anxiety accompanied with facial spots diseases were obtained by making Venn diagram respectively. Protein-protein interaction (PPI), Gene Ontology (GO) and Kyoto Encyclopedia of genes and genomes (KEGG) pathway enrichment analysis of intersection targets were carried out through STRING platform, Metascape and KEGG Mapper databases. The "complex essential oil- component- target- pathway" network was constructed by Cytoscape software.The key active compounds and core targets were docked by AutoDock software.

Results: A total of 34 active components of complex rose-bergamot essential oil and 405 corresponding targets were obtained. The intersection targets of complex essential oil with anxiety disorder and facial spot were 88 and 34 respectively, and the related KEGG pathways were 137 and 123 items respectively. Neroli acetate, Neroli alcohol and Linalool in complex rose- bergamot essential oil could play a therapeutic role in anxiety disorder by regulating key targets such as SLC6A4, GRIN2B and DRD2 to participate in the neuroactive ligand receptor interaction and serotonergic synapse and other pathways, they also could play a role in the treatment of facial spots by regulating key targets such as EGFR, VEGFA and PIK3CA in the PI3K-AKT, JAK-STAT and other signal pathways. The molecular docking between the key compound and the core target showed that the binding energy was less than 0kcal / mol, indicating that the compound and the target could bind stably.

Conclusion: Complex rose-bergamot essential oil can play a role in the treatment of anxiety disorder with facial spots through the synergistic mechanism of multi-component, multi-target and multi-pathway.
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http://dx.doi.org/10.1111/jocd.15296DOI Listing
August 2022

Selective transplantation method of leafy vegetable seedlings based on ResNet 18 network.

Front Plant Sci 2022 22;13:893357. Epub 2022 Jul 22.

College of Agricultural Equipment Engineering, Henan University of Science and Technology, Luoyang, China.

To solve the problem of low survival rate caused by unscreened transplanting of seedlings. This study proposed a selective transplanting method of leafy vegetable seedlings based on the ResNet 18 network. Lettuce seedlings were selected as the research object, and a total of 3,388 images were obtained in the dataset. The images were randomly divided into the training set, validation set, and test set in the ratio of 6:2:2. The ResNet 18 network was used to perform transfer learning after tuning, identifying, and classifying leafy vegetable seedlings, and then establishing a model to screen leafy vegetable seedlings. The results showed that the optimal detection accuracy of the presence and health of seedlings in the training data set was above 100%, and the model loss remained at around 0.005. Nine hundred seedlings were selected for the validation test, and the screening accuracy rate was 97.44%, the precision rate of healthy seedlings was 97.56%, the recall rate was 97.34%, the precision rate of unhealthy seedlings was 92%, and the recall rate was 92.62%, which was better than the screening model based on the physical characteristics of seedlings. If they were identified as unhealthy seedlings, the manipulator would remove them during the transplanting process and perform the seedling replenishment operation to increase the survival rate of the transplanted seedlings. Moreover, the seedling image is extracted by background removal technology, so the model processing time for a single image is only 0.0129 s. This research will provide technical support for the selective transplantation of leafy vegetable seedlings.
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http://dx.doi.org/10.3389/fpls.2022.893357DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355090PMC
July 2022

Prediction of protein secondary structure based on an improved channel attention and multiscale convolution module.

Front Bioeng Biotechnol 2022 22;10:901018. Epub 2022 Jul 22.

Engineering Research Center of Cyberspace, Yunnan University, Kunming, Yunnan, China.

Prediction of the protein secondary structure is a key issue in protein science. Protein secondary structure prediction (PSSP) aims to construct a function that can map the amino acid sequence into the secondary structure so that the protein secondary structure can be obtained according to the amino acid sequence. Driven by deep learning, the prediction accuracy of the protein secondary structure has been greatly improved in recent years. To explore a new technique of PSSP, this study introduces the concept of an adversarial game into the prediction of the secondary structure, and a conditional generative adversarial network (GAN)-based prediction model is proposed. We introduce a new multiscale convolution module and an improved channel attention (ICA) module into the generator to generate the secondary structure, and then a discriminator is designed to conflict with the generator to learn the complicated features of proteins. Then, we propose a PSSP method based on the proposed multiscale convolution module and ICA module. The experimental results indicate that the conditional GAN-based protein secondary structure prediction (CGAN-PSSP) model is workable and worthy of further study because of the strong feature-learning ability of adversarial learning.
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http://dx.doi.org/10.3389/fbioe.2022.901018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355137PMC
July 2022

Evaluation of water quality at national scale from 2011 to 2021: Advances and challenges.

Sci Total Environ 2022 Aug 4:157803. Epub 2022 Aug 4.

State Key Laboratory on Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P. O. Box 2871, Beijing 100085, PR China.

More environmental policies and larger investments in protecting the aquatic environment in China have been made in the last decade than previously. It is important to assess how this will affect river water quality. Here, changes in water quality in China between 2011 and 2021 are assessed. Water bodies meeting class III or better defined in the Chinese Environmental Quality Standards for Surface Water (GB3838-2002) were labeled WQI, water bodies meeting class V or better but below class III were labeled WQII, and water bodies below class V were labeled WQIII. The percentage of WQI water bodies increased from 66.1 % in 2011 to 81.0 % in 2021, and the percentages of WQII and WQIII water bodies decreased between 2011 and 2021. The percentage of WQI water bodies increased more quickly and the percentage WQIII water bodies decreased more quickly after 2017 than between 2011 and 2016. The percentages of WQI water bodies in the Northwest River Basin (RB), Pearl RB, Southeast RB, Southwest RB, and Yangtze RB were >80 %, and were higher than the percentages of WQI water bodies in the other five RBs. The percentages of WQI and WQII water bodies increased but the percentage of WQIII water bodies decreased in the Hai RB. The percentage of WQI water bodies increased but the percentages of WQII and WQIII water bodies decreased in the Huai RB, Liao RB, Yangtze RB, and Yellow RB. The river monitoring capacity increased and pollution sources, particularly point sources, became more controlled, and this improved river water quality. River management in China has passed the first stage of controlling pollution sources after 10 years of centralized management. The next stage should be focused on strengthening control of non-point sources of pollution and rehabilitating ecological systems to improve river health.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157803DOI Listing
August 2022

A 5-year retrospective analysis of the risk factors, treatment and prognosis of patients with fungal keratitis in Heilongjiang.

Am J Ophthalmol 2022 Aug 3. Epub 2022 Aug 3.

Eye Hospital, The First Affiliated Hospital of Harbin Medical University, Key Laboratory of Basic and Clinical Research of Heilongjiang Province, Harbin, Heilongjiang Province, People's Republic of China. Electronic address:

Purpose: To report the epidemiological features, laboratory findings, and treatment outcomes of patients with fungal keratitis (FK) during a busy farming period (May, June, September, October and November) in Heilongjiang Province, China.

Design: Retrospective, observational case series.

Methods: In total, 251 patients diagnosed with FK at the Eye Hospital, First Affiliated Hospital of Harbin Medical University (HMU), from 2017 to 2021 were identified. Medical records were retrospectively analyzed, and demographic features, risk factors, monthly distributions, clinical characteristics, laboratory findings, treatment strategies and prognostic data were collected.

Results: The number of FK cases in the busy farming period accounted for 74.1% (186/251) of the total. The mean patient age was 58.2±9.5 years. Males (66.7%) were more likely to develop FK than females (33.3%), and plant-related trauma was the main cause in 80.1% (149/186) of the cases. The most common causative fungal species was Fusarium (34.9%). In vivo confocal microscopy (IVCM) had the highest positivity rate for FK diagnosis (94.6%). The depth of hypopyon, depth of hyphae or spores and infiltrate width (IW) were significantly positively correlated with delayed presentation.

Conclusions: Patients with a longer time of presentation to the hospital were more likely to undergo surgery due to topical medical therapy failure. The most important measures for FK prevention are the use of eye protection during outdoor work and visiting the hospital for treatment as soon as possible after eye injury.
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http://dx.doi.org/10.1016/j.ajo.2022.07.023DOI Listing
August 2022

EGF promotes PKM2 O-GlcNAcylation by stimulating O-GlcNAc transferase phosphorylation at Y976 and their subsequent association.

J Biol Chem 2022 Aug 2:102340. Epub 2022 Aug 2.

Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, 5268 Renmin Street, Changchun, Jilin, 130024, China. Electronic address:

Epidermal growth factor (EGF) is one of the most well-characterized growth factors and plays a crucial role in cell proliferation and differentiation. Its receptor EGFR has been extensively explored as a therapeutic target against multiple types of cancers, such as lung cancer and glioblastoma. Recent studies have established a connection between deregulated EGF signaling and metabolic reprogramming, especially rewiring in aerobic glycolysis, which is also known as the Warburg effect and recognized as a hallmark in cancer. Pyruvate kinase M2 (PKM2) is a rate-limiting enzyme controlling the final step of glycolysis and serves as a major regulator of the Warburg effect. We previously showed that PKM2 T405/S406 O-GlcNAcylation, a critical mark important for PKM2 de-tetramerization and activity, was markedly upregulated by EGF. However, the mechanism by which EGF regulates PKM2 O-GlcNAcylation still remains uncharacterized. Here, we demonstrated that EGF promoted O-GlcNAc transferase (OGT) binding to PKM2 by stimulating OGT Y976 phosphorylation. As a consequence, we found PKM2 O-GlcNAcylation and de-tetramerization were upregulated, leading to a significant decrease in PKM2 activity. Moreover, distinct from PKM2, we observed that the association of additional phosphotyrosine binding proteins with OGT was also enhanced when Y976 was phosphorylated. These proteins included STAT1, STAT3, STAT5, PKCδ, and p85, which are reported to be O-GlcNAcylated. Together, we show EGF-dependent Y976 phosphorylation is critical for OGT-PKM2 interaction and propose that this post-translational modification might be important for substrate selection by OGT.
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http://dx.doi.org/10.1016/j.jbc.2022.102340DOI Listing
August 2022

LncRNA serves as a competing endogenous RNA of to upregulate in papillary thyroid carcinoma.

Arch Endocrinol Metab 2022 Aug 4. Epub 2022 Aug 4.

Department of Pathology, Affiliated 3201 Hospital of Xi'an Jiaotong University, Hanzhong City, Shaanxi Province, PR China.

Objective: has been characterized as an oncogenic long non-coding RNA (lncRNA) in several cancers including papillary thyroid cancer (PTC), but its role in PTC has not been fully elucidated. Considering the critical role of lncRNAs in cancer biology, further functional analysis of in PTC may provide novel insights into PTC management.

Subjects And Methods: Paired tumor and non-tumor tissues were collected from 63 papillary thyroid carcinoma (PTC) patients. Expression levels of and in tissue samples were analyzed by qRT-PCR. Cell transfection was performed to explore the interactions among and . Cell proliferation assay was performed to evaluate the effects of and on cell proliferation.

Results: accumulated to high levels in PTC tissues and was affected by clinical stage. showed a positive correlation with across PTC tissues. RNA interaction prediction showed that could bind to , which can directly target . and showed no regulatory role regulating the expression of each other, but overexpression of upregulated and enhanced cell proliferation. In contrast, overexpression of miR-218 downregulated and attenuated cell proliferation. In addition, miR-218 suppressed the role of in regulating the expression of and cell proliferation.

Conclusion: may serve as a competing endogenous RNA of to upregulate in PTC, thereby promoting cell proliferation. The pathway characterized in the present study might serve as a potential target to treat PTC.
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http://dx.doi.org/10.20945/2359-3997000000510DOI Listing
August 2022

Postoperative high-density lipoprotein cholesterol level: an independent prognostic factor for gastric cancer.

Front Oncol 2022 18;12:884371. Epub 2022 Jul 18.

Senior Department of Oncology, The Fifth Medical Center of People's Liberation Army (PLA) General Hospital, Beijing, China.

Objective: The relationship between serum lipids and prognosis of gastric cancer has not been confirmed. Our purpose in the study was to investigate the associations between preoperative and postoperative serum lipids level and prognosis in patients with gastric cancer.

Methods: A retrospective study was performed on 431 patients who received radical (R0) gastrectomy from 2011 to 2013. Preoperative and postoperative serum lipids level were recorded. Clinical-pathological characteristics, oncologic outcomes, disease-free survival (DFS) and overall survival (OS) were collected. The prognostic significance was determined by Kaplan-Meier analysis and Cox proportional hazards regression model.

Results: There was no significant difference in DFS and OS according to preoperative serum lipids level. Regarding postoperative serum lipids level, compared to normal high-density lipoprotein cholesterol (HDL-C), low postoperative HDL-C level indicated a shorter OS (hazard ratio: 1.76, 99% confidence interval: 1.31-2.38; P=0.000) and a shorter DFS (hazard ratio: 2.06, 99% confidence interval: 1.55-2.73; P=0.000). However, other postoperative serum lipid molecules were not associated with DFS and OS.

Conclusion: Postoperative HDL-C might be an independent prognostic factor of gastric cancer.
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http://dx.doi.org/10.3389/fonc.2022.884371DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339602PMC
July 2022

Feature Genes in Neuroblastoma Distinguishing High-Risk and Non-high-Risk Neuroblastoma Patients: Development and Validation Combining Random Forest With Artificial Neural Network.

Front Med (Lausanne) 2022 15;9:882348. Epub 2022 Jul 15.

Department of General Surgery, Guiqian International General Hospital, Guiyang, China.

There is a significant difference in prognosis among different risk groups. Therefore, it is of great significance to correctly identify the risk grouping of children. Using the genomic data of neuroblastoma samples in public databases, we used GSE49710 as the training set data to calculate the feature genes of the high-risk group and non-high-risk group samples based on the random forest (RF) algorithm and artificial neural network (ANN) algorithm. The screening results of RF showed that EPS8L1, PLCD4, CHD5, NTRK1, and SLC22A4 were the feature differentially expressed genes (DEGs) of high-risk neuroblastoma. The prediction model based on gene expression data in this study showed high overall accuracy and precision in both the training set and the test set (AUC = 0.998 in GSE49710 and AUC = 0.858 in GSE73517). Kaplan-Meier plotter showed that the overall survival and progression-free survival of patients in the low-risk subgroup were significantly better than those in the high-risk subgroup [HR: 3.86 (95% CI: 2.44-6.10) and HR: 3.03 (95% CI: 2.03-4.52), respectively]. Our ANN-based model has better classification performance than the SVM-based model and XGboost-based model. Nevertheless, more convincing data sets and machine learning algorithms will be needed to build diagnostic models for individual organization types in the future.
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http://dx.doi.org/10.3389/fmed.2022.882348DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9336509PMC
July 2022

High lignin, light-driven shape memory polymers with excellent mechanical performance.

Int J Biol Macromol 2022 Jul 26;219:44-52. Epub 2022 Jul 26.

Key Laboratory of Bio-based Material Science & Technology, Northeast Forestry University, Ministry of Education, Harbin 150040, PR China; College of Material Science and Engineering, Northeast Forestry University, Harbin 150040, PR China. Electronic address:

With the gradual global standardization of carbon emission policies, the development of renewable resources to replace traditional fossil resources is assuming increasing importance. Lignin is the most abundant natural source of aromatic compounds and has the potential to replace petroleum-based aromatic hydrocarbons. In this work, the rigid benzene ring structure and excellent photothermal properties of lignin were exploited to produce light-driven lignin-based shape memory polymers (ELEPs) that contain high proportions of lignin and have good mechanical properties. Enzymatically hydrolyzed lignin (EL), epoxy soybean oil (ESO) and polyethylene glycol (PEG 400) were copolymerized and cured to form ELEPs, which have a disordered three-dimensional network. An increase in the proportion of EL from 40 to 60 wt% enhanced the mechanical properties, as reflected by an increase in tensile strength from 11.3 to 30.8 MPa and in the glass transition temperature (T) from 93 to 115.7 °C. Under simulated solar irradiation (2000 W m), ELEP50, which contains 50 wt% lignin and has a T of 105 °C, reached a surface temperature as high as 105 °C and achieved shape memory within 20 s. The shape fixation ratio (R) and shape recovery ratio (R) were stably >98 % and >97 %, respectively, over eight cycles in a bending-recovery experiment. The unique light-driven shape memory properties of ELEPs provide a method for high value utilization of EL, and the design strategy offers new ideas for producing novel intelligent materials.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.07.134DOI Listing
July 2022

Comparing "people-like-me" and linear mixed model predictions of functional recovery following knee arthroplasty.

J Am Med Inform Assoc 2022 Jul 28. Epub 2022 Jul 28.

VA Eastern Colorado Geriatric Research, Education, and Clinical Center (GRECC), VA Eastern Colorado Health Care System, Aurora, Colorado, USA.

Objective: Prediction models can be useful tools for monitoring patient status and personalizing treatment in health care. The goal of this study was to compare the relative strengths and weaknesses of 2 different approaches for predicting functional recovery after knee arthroplasty: a neighbors-based "people-like-me" (PLM) approach and a linear mixed model (LMM) approach.

Materials And Methods: We used 2 distinct datasets to train and then test PLM and LMM prediction approaches for functional recovery following knee arthroplasty. We used the Timed Up and Go (TUG)-a common test of mobility-to operationalize physical function. Both approaches used patient characteristics and baseline postoperative TUG values to predict TUG recovery from days 1-425 following surgery. We then compared the accuracy and precision of PLM and LMM predictions.

Results: A total of 317 patient records with 1379 TUG observations were used to train PLM and LMM approaches, and 456 patient records with 1244 TUG observations were used to test the predictions. The approaches performed similarly in terms of mean squared error and bias, but the PLM approach provided more accurate and precise estimates of prediction uncertainty.

Discussion And Conclusion: Overall, the PLM approach more accurately and precisely predicted TUG recovery following knee arthroplasty. These results suggest PLM predictions may be more clinically useful for monitoring recovery and personalizing care following knee arthroplasty. However, clinicians and organizations seeking to use predictions in practice should consider additional factors (eg, resource requirements) when selecting a prediction approach.
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http://dx.doi.org/10.1093/jamia/ocac123DOI Listing
July 2022

A three-dimensional flow model of screen channel liquid acquisition devices for propellant management in microgravity.

NPJ Microgravity 2022 Jul 28;8(1):28. Epub 2022 Jul 28.

Institute of Refrigeration and Cryogenics, Shanghai Jiao Tong University, Shanghai, 200240, China.

Screen channel liquid acquisition devices (LADs) are among the most promising technologies for separating liquid and vapor phases in propellant storage tanks under microgravity conditions and thus ensuring vapor-free propellant supply to spacecraft engines. However, the prediction of the critical flow rate of a screen channel LAD relies on the full understanding of the three dimensional distribution of injection velocity. In this study, the flow characteristics at the entrance region of the LAD were investigated via particle image velocimetry (PIV) technique and numerical simulations under various working conditions. The experimental results illustrated that the velocity component normal to the porous woven mesh is non-uniform in both streamwise and spanwise directions of channel flow and that this phenomenon has a significant influence on the critical flow rate. Hence, a model that accounts for the three-dimensional flow field was proposed to predict the critical flow rate. The average error in the critical flow rate, which was determined by comparing the proposed model's predictions and the experimental results, was less than 8.4%.
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http://dx.doi.org/10.1038/s41526-022-00216-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334641PMC
July 2022

Residual Hearing Improves Early Auditory Perception and Speech Intelligibility in Mandarin-Speaking Children with Cochlear Implants.

J Int Adv Otol 2022 Jul;18(4):291-296

Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Department of Otolaryngology, Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, 100045, China.

Background: To investigate the relationship between residual hearing and early auditory speech performance in Mandarin-speaking children with cochlear implants.

Methods: Twenty-four prelingually deaf children implanted with a cochlear implants participated in the study. Auditory performance and speech intelligibility were longitudinally evaluated by categories of auditory performance, infant-toddler meaningful auditory integration scale/ meaningful auditory integration scale, speech intelligibility rating, and meaningful use of speech scale. According to the postoperative pure tone average threshold, children were grouped as "better" and "worse" residual hearing.

Results: Better hearing preservation was observed in 7 children (29.2%) and worse preservation in 17 children (70.8%). The scores of categories of auditory performance, infant-toddler meaningful auditory integration scale/meaningful auditory integration scale, speech intelligibility rating, and meaningful use of speech scalewere higher in children with better residual hearing. The residual hearing level was significantly associated with the performance of meaningful use of speech scale (P = .004), as well as the performance of speech intelligibility rating (P = .049).

Conclusion: The present study showed that children with better residual hearing exhibited advantages in the early auditory and speech out- comes. The study highlighted the effects of residual hearing on early auditory performance and speech intelligibility development in Mandarin- speaking children with cochlear implants.
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http://dx.doi.org/10.5152/iao.2022.21442DOI Listing
July 2022

Design, Synthesis and Evaluation of Novel Phorbazole C Derivatives as MNK Inhibitors through Virtual High-Throughput Screening.

Mar Drugs 2022 Jun 29;20(7). Epub 2022 Jun 29.

School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.

MNKs (mitogen-activated protein kinase-interacting protein kinases) phosphorylate eIF4E at Ser209 to control the translation of certain mRNAs and regulate the process of cell proliferation, cell migration and invasion, etc. Development of MNK inhibitors would be an effective treatment for related diseases. We used the MarineChem3D database to identify hit compounds targeting the protein MNK1 and MNK2 through high-throughput screening. Compounds from the phorbazole family showed good interactions with MNK1, and phorbazole C was selected as our hit compound. By analyzing the binding mode, we designed and synthesized 29 derivatives and evaluated their activity against MNKs, of which, six compounds showed good inhibition to MNKs. We also confirmed three interactions between this kind of compound and MNK1, which are vital for the activity. In conclusion, we report series of novel MNK inhibitors inspired from marine natural products and their relative structure-activity relationship. This will provide important information for further developing MNK inhibitors based on this kind of structure.
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http://dx.doi.org/10.3390/md20070429DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319845PMC
June 2022

Umbilical Cord Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Deliver miR-21 to Promote Corneal Epithelial Wound Healing through PTEN/PI3K/Akt Pathway.

Stem Cells Int 2022 14;2022:1252557. Epub 2022 Jul 14.

Eye Hospital, First Affiliated Hospital, Harbin Medical University, Harbin, China.

Objective: Rapid restoration of corneal epithelium integrity after injury is particularly important for preserving corneal transparency and vision. Mesenchymal stem cells (MSCs) can be taken into account as the promising regenerative therapeutics for improvement of wound healing processes based on the variety of the effective components. The extracellular vesicles form MSCs, especially exosomes, have been considered as important paracrine mediators though transferring microRNAs into recipient cell. This study investigated the mechanism of human umbilical cord MSC-derived small extracellular vesicles (HUMSC-sEVs) on corneal epithelial wound healing.

Methods: HUMSC-sEVs were identified by transmission electron microscopy, nanoparticle tracking analysis, and Western blot. Corneal fluorescein staining and histological staining were evaluated in a corneal mechanical wound model. Changes in HCEC proliferation after HUMSC-sEVs or miR-21 mimic treatment were evaluated by CCK-8 and EdU assays, while migration was assessed by in vitro scratch wound assay. Full-length transcriptome sequencing was performed to identify the differentially expressed genes associated with HUMSC-sEVs treatment, followed by validation via real-time PCR and Western blot.

Results: The sEVs derived from HUMSCs can significantly promote corneal epithelial cell proliferation, migration in vitro, and corneal epithelial wound healing in vivo. Similar effects were obtained after miR-21 transfection, while the beneficial effects of HUMSC-sEVs were partially negated by miR-21 knockdown. Results also show that the benefits are associated with decreased PTEN level and activated the PI3K/Akt signaling pathway in HCECs.

Conclusion: HUMSC-sEVs could enhance the recovery of corneal epithelial wounds though restraining PTEN by transferring miR-21 and may represent a promising novel therapeutic agent for corneal wound repair.
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http://dx.doi.org/10.1155/2022/1252557DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303509PMC
July 2022

CMDB: the comprehensive population genome variation database of China.

Nucleic Acids Res 2022 Jul 25. Epub 2022 Jul 25.

BGI-Shenzhen, Shenzhen518083, Guangdong, China.

A high-quality genome variation database derived from a large-scale population is one of the most important infrastructures for genomics, clinical and translational medicine research. Here, we developed the Chinese Millionome Database (CMDB), a database that contains 9.04 million single nucleotide variants (SNV) with allele frequency information derived from low-coverage (0.06×-0.1×) whole-genome sequencing (WGS) data of 141 431 unrelated healthy Chinese individuals. These individuals were recruited from 31 out of the 34 administrative divisions in China, covering Han and 36 other ethnic minorities. CMDB, housing the WGS data of a multi-ethnic Chinese population featuring wide geographical distribution, has become the most representative and comprehensive Chinese population genome database to date. Researchers can quickly search for variant, gene or genomic regions to obtain the variant information, including mutation basic information, allele frequency, genic annotation and overview of frequencies in global populations. Furthermore, the CMDB also provides information on the association of the variants with a range of phenotypes, including height, BMI, maternal age and twin pregnancy. Based on these data, researchers can conduct meta-analysis of related phenotypes. CMDB is freely available at https://db.cngb.org/cmdb/.
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http://dx.doi.org/10.1093/nar/gkac638DOI Listing
July 2022

Antidiarrheal activity of the extracts of Valeriana jatamansi Jones on castor oil-induced diarrhea mouse by regulating multiple signal pathways.

J Ethnopharmacol 2022 Jul 18:115560. Epub 2022 Jul 18.

Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai, 201203, PR China. Electronic address:

Ethnopharmacological Relevance: Valeriana jatamansi Jones, a traditional medicine, is used for various medicinal purposes worldwide. This species is popular for its gastro-protective properties and has been verified to exert antidiarrheal effects. Qiuxieling mixture, an oral liquid preparation used to treat diarrhea in children in clinical practice, was extracted from V. jatamansi Jones.

Aim Of The Study: Although Qiuxieling mixture has a good preventive effect on diarrhea children, the disgusting smell makes it intolerable. Therefore, we extracted odorless products from V. jatamansi Jones and Qiuxieling mixture. The present study is aimed to investigate the protective effects of two ethanolic extracts of V. jatamansi Jones and Qiuxieling mixture against castor oil-induced diarrhea and their possible mechanisms in mice.

Materials And Methods: The two extracts of V. jatamansi Jones and Qiuxieling mixture were detected by HPLC. A castor oil-induced diarrheal model was used to evaluate the antidiarrheal effects. The expression of Occludin in the small intestine was measured by IHC. Western blotting and immunofluorescence were used to detect the expression of proteins related to the oxidative stress and GSDMD-mediated pyroptosis signaling pathways. ELISA was used to detect the expression of IL-6 and IL-1β in the small intestine of mice with diarrhea.

Results: The two extracts of V. jatamansi Jones and Qiuxieling mixture dose-dependently reduced the diarrhea index and the diarrhea rate, delayed the onset of diarrhea, and decreased the weight of the intestinal content. Meanwhile, they reversed the decreased expression of Occludin and restored the activity of Na-K-ATPase in the intestines of diarrheal mice. In addition, they reversed the depletion of GSH, attenuated the activation of the ERK/JNK pathway, promoted the Nrf2/SOD1 signaling pathways, and decreased the release of ROS in the intestines of diarrheal mice. Moreover, they suppressed GSDMD-mediated pyroptosis by downregulating the NLRP3/caspase-1/GSDMD signaling pathway.

Conclusions: The two extracts of V. jatamansi Jones and Qiuxieling mixture exerted protective effects on castor oil-induced diarrhea in mice through a variety of mechanisms, including antioxidant stress, restoration of tight junctions between intestinal mucosal cells and regulation of the GSDMD-mediated pyroptosis pathway.
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http://dx.doi.org/10.1016/j.jep.2022.115560DOI Listing
July 2022

A Self-Reporting Fluorescent Salicylaldehyde-Chlorambucil Conjugate as a Type-II ICD Inducer for Cancer Vaccines.

Adv Mater 2022 Jul 21:e2205701. Epub 2022 Jul 21.

School of Science and Engineering, Shenzhen Key Laboratory of Functional Aggregate Materials, The Chinese University of Hong Kong, Shenzhen, Guangdong, 518172, China.

Immunogenic cell death (ICD) can activate the anticancer immune response and is highly attractive to improve cancer treatment efficacy. ICD is closely related to endoplasmic reticulum (ER) stress, and a series of ICD inducers has recently been reported based on ER-targeted photodynamic/photothermal agents or metal complexes. However, these ER-targeted ICD inducers suffer from complicated synthesis and heavy-metal cytotoxicity. Inspired by the promising clinical potential of small organic molecules, herein, an ER-targeted fluorescent self-reporting ICD inducer, SA-Cbl, is developed by simple conjugation of the chemotherapeutic drug chlorambucil (Cbl) with salicylaldehyde (SA). SA-Cbl can selectively accumulate in the ER to induce rapid ROS generation and an unfolded protein response process, which leads to a fast release of damage-associated molecular patterns and efficient dendritic cells maturation. Meanwhile, the ER-targeted accumulation and ER-stress-inducing process can be in situ monitored based on the turn-on fluorescence of SA-Cbl, which is highly pH- and polarity-sensitive and can selectively interact with ER proteins. Compared with the traditional chemotherapy drug doxorubicin, the superior anticancer immunity effect of SA-Cbl is verified via an in vivo tumor model. This study thus provides a new strategy for developing fluorescent self-reporting ICD inducers by decoration of chemotherapeutic drugs with pH and polarity-sensitive organic fluorophores.
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http://dx.doi.org/10.1002/adma.202205701DOI Listing
July 2022

Neurotensin orchestrates valence assignment in the amygdala.

Nature 2022 Jul 20. Epub 2022 Jul 20.

Division of Depression and Anxiety Disorders, McLean Hospital, Belmont, MA, USA.

The ability to associate temporally segregated information and assign positive or negative valence to environmental cues is paramount for survival. Studies have shown that different projections from the basolateral amygdala (BLA) are potentiated following reward or punishment learning. However, we do not yet understand how valence-specific information is routed to the BLA neurons with the appropriate downstream projections, nor do we understand how to reconcile the sub-second timescales of synaptic plasticity with the longer timescales separating the predictive cues from their outcomes. Here we demonstrate that neurotensin (NT)-expressing neurons in the paraventricular nucleus of the thalamus (PVT) projecting to the BLA (PVT-BLA:NT) mediate valence assignment by exerting NT concentration-dependent modulation in BLA during associative learning. We found that optogenetic activation of the PVT-BLA:NT projection promotes reward learning, whereas PVT-BLA projection-specific knockout of the NT gene (Nts) augments punishment learning. Using genetically encoded calcium and NT sensors, we further revealed that both calcium dynamics within the PVT-BLA:NT projection and NT concentrations in the BLA are enhanced after reward learning and reduced after punishment learning. Finally, we showed that CRISPR-mediated knockout of the Nts gene in the PVT-BLA pathway blunts BLA neural dynamics and attenuates the preference for active behavioural strategies to reward and punishment predictive cues. In sum, we have identified NT as a neuropeptide that signals valence in the BLA, and showed that NT is a critical neuromodulator that orchestrates positive and negative valence assignment in amygdala neurons by extending valence-specific plasticity to behaviourally relevant timescales.
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http://dx.doi.org/10.1038/s41586-022-04964-yDOI Listing
July 2022

Occupational Differences in Psychological Distress Between Chinese Dentists and Dental Nurses.

Front Psychol 2022 1;13:923626. Epub 2022 Jul 1.

Key Laboratory of Psychoseomadsy, Stomatological Hospital of Chongqing Medical University, Chongqing, China.

Background: Doctors and allied health professionals are facing serious mental health issues, which have received widespread attention. This study aimed to explore the occupational differences in psychological distress between Chinese dentists and dental nurses.

Materials And Methods: The data was collected from a cross-sectional study conducted by the Chongqing Stomatological Association. Medical personnel involved in this survey were invited to complete a battery of self-administrated questionnaires, specifically the General Health Questionnaire-12, Maslach Burnout Inventory, and career choice regret scale. Data on demographic characteristics and working conditions were also collected. The results of these questionnaires were analyzed with SPSS (version 23.0). Univariate and multivariable analyzes were conducted to explore the influencing factors.

Results: A total of 3,020 valid questionnaires, including 1,855 dentists and 1,165 dental nurses, were collected from 11 provinces of China. In general, 23.8% of responders exhibited psychological distress. The rate of dentists was 25.7%, and that of dental nurses was 20.8%. The prevalence was 4.9% higher in dentists than in dental nurses ( < 0.05). The multivariable analysis showed that factors associated with psychological distress for dentists were lower income, burnout, high job stress, career-choice regret, and lack of sufficient personal time, and that for dental nurses were age, lower income, longer working hours per week, burnout, high job stress, low job satisfaction, lack of sufficient personal time, and poor medical environment.

Conclusion: The prevalence of psychological distress was relatively high among dental medical staff, and dentists showed a higher prevalence than dental nurses. Nurses have more risk factors for psychological distress than dentists. These results indicate that it is necessary to monitor the mental health status of dental medical staff and implement accurate strategies for dentists and dental nurses to promote their physical and mental health.
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http://dx.doi.org/10.3389/fpsyg.2022.923626DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9285401PMC
July 2022

Non-Invasive Prenatal Diagnosis of Monogenic Disorders Through Bayesian- and Haplotype-Based Prediction of Fetal Genotype.

Front Genet 2022 1;13:911369. Epub 2022 Jul 1.

Prenatal Diagnosis Centre, Guangdong Women and Children's Hospital, Guangzhou, China.

Non-invasive prenatal diagnosis (NIPD) can identify monogenic diseases early during pregnancy with negligible risk to fetus or mother, but the haplotyping methods involved sometimes cannot infer parental inheritance at heterozygous maternal or paternal loci or at loci for which haplotype or genome phasing data are missing. This study was performed to establish a method that can effectively recover the whole fetal genome using maternal plasma cell-free DNA (cfDNA) and parental genomic DNA sequencing data, and validate the method's effectiveness in noninvasively detecting single nucleotide variations (SNVs), insertions and deletions (indels). A Bayesian model was developed to determine fetal genotypes using the plasma cfDNA and parental genomic DNA from five couples of healthy pregnancy. The Bayesian model was further integrated with a haplotype-based method to improve the inference accuracy of fetal genome and prediction outcomes of fetal genotypes. Five pregnancies with high risks of monogenic diseases were used to validate the effectiveness of this haplotype-assisted Bayesian approach for noninvasively detecting indels and pathogenic SNVs in fetus. Analysis of healthy fetuses led to the following accuracies of prediction: maternal homozygous and paternal heterozygous loci, 96.2 ± 5.8%; maternal heterozygous and paternal homozygous loci, 96.2 ± 1.4%; and maternal heterozygous and paternal heterozygous loci, 87.2 ± 4.7%. The respective accuracies of predicting insertions and deletions at these types of loci were 94.6 ± 1.9%, 80.2 ± 4.3%, and 79.3 ± 3.3%. This approach detected pathogenic single nucleotide variations and deletions with an accuracy of 87.5% in five fetuses with monogenic diseases. This approach was more accurate than methods based only on Bayesian inference. Our method may pave the way to accurate and reliable NIPD.
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http://dx.doi.org/10.3389/fgene.2022.911369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283829PMC
July 2022

Recent Progress and Future Perspectives of Immunotherapy in Advanced Gastric Cancer.

Front Immunol 2022 1;13:948647. Epub 2022 Jul 1.

Department of Gastrointestinal Surgery, Changhai Hospital, Naval Medical University, Shanghai, China.

As one of the most common forms of solid tumours, gastric carcinoma has been revealed as the third leading cause of death worldwide. The symptom of gastric cancer is usually not obvious and thus difficult to detect at earlier stages. Therefore, gastric cancer is already in the advanced stage once detected in patients, which has a poor prognosis due to ineffective therapies and multiple resistance. Recent advance in understanding the microenvironment of cancer has significantly promoted the development of immunotherapy for advanced gastric cancer. Immunotherapy can induce immune responses in gastric cancer patients thus leads to the destruction of cancer cells. In comparison of traditional therapy, immunotherapy has demonstrated robust efficacy and tolerable toxicity. Therefore, this novel strategy for treatment of advanced gastric cancer has gain increasingly popularity. In this review, we summarize recent progress of immunotherapy in advanced gastric cancer, such as immune check point inhibitors, adoptive cell therapy, VEGF inhibitors, cancer vaccines and CAR-T cell therapy. We highlight immunotherapies involved in clinical applications and discuss the existing challenges of current immunotherapies and promising strategies to overcome these limitations.
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http://dx.doi.org/10.3389/fimmu.2022.948647DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284215PMC
July 2022

The Value of Metagenomic Next-Generation Sequencing in Hematological Malignancy Patients with Febrile Neutropenia After Empiric Antibiotic Treatment Failure.

Infect Drug Resist 2022 7;15:3549-3559. Epub 2022 Jul 7.

Department of Hematology, Tianjin First Central Hospital, Tianjin, 300192, People's Republic of China.

Background: It was crucial to use empirical antibiotics in febrile neutropenia (FN) patients. However, most patients still died from infection due to poor efficacy. Metagenomic next-generation sequencing (mNGS) is a rapid microbiological diagnostic method. The value of mNGS in patients with FN remains to be studied, especially after empiric antibiotic treatment.

Methods: We retrospectively analyzed the differences between mNGS and the traditional methods in 192 patients with hematological malignancies who have received empiric antibiotic treatment. Samples were collected when patient had chills or half an hour before peak body temperature. And we compared the differences between FN and non-FN patients, mainly including types of pathogens and the diagnostic value of different pathogens.

Results: Despite receiving empirical treatment, the pathogen detection rate of mNGS was significantly higher than the traditional method (80.21% vs 25.00%, <0.001). And it has obvious advantages in detecting mixed pathogens infection (80.21% vs 4.17%, <0.001). Then, we found that mNGS saw more pathogens in the FN than in the non-FN group, especially fungus. 21/33 (63.63%) of FN patients was diagnosed with fungal infections. The fungal detection rate in FN was significantly higher than non-FN group (32.35% vs 12.22%, =0.001). Besides, the sensitivity of mNGS was higher than the traditional methods in both FN and non-FN group (<0.001), but no significant difference in specificity (>0.05). In the FN group, empiric antibiotic treatment of 46/102 (45.10%) patients did not treat all the pathogens detected by mNGS. After adjusting the antimicrobial regimen according to the results of mNGS, the effective rate at 72 hours and 7 days was 22/46 (47.83%) and 24/102 (52.17%), respectively.

Conclusion: mNGS had a significant impact on the diagnosis of infection and the second-line antimicrobial therapy in FN. mNGS plays a more important role in FN patients, especially in the diagnosis of fungal infections.

Purpose: Firstly, we compared the difference between mNGS and the traditional methods in the diagnosis of infection. Secondly, we assessed the value of mNGS in FN patients by comparing it with non-FN patients, including types of pathogens and the diagnostic value of different pathogens. In order to show that mNGS plays a more important role in FN.
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http://dx.doi.org/10.2147/IDR.S364525DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273631PMC
July 2022

Cumulative Family Risk and Cyberbullying Among Chinese Adolescents: The Chain Mediating Role of School Connectedness and Cyber Victimization.

Front Public Health 2022 27;10:898362. Epub 2022 Jun 27.

Department of Psychology, College of Education and Sports Sciences, Yangtze University, Jingzhou, China.

Abundant evidence has demonstrated that cumulative family risk is associated with cyberbullying. However, few studies to date have investigated how cumulative family risk links to cyberbullying. To fill in these gaps, the present study examined the mediating role of school connectedness and cyber victimization in the relation between cumulative family risk and cyberbullying. A sample of 1,804 Chinese adolescents was recruited to complete measures of cumulative family risk, cyberbullying, school connectedness, cyber victimization, and demographic variables through convenience sampling. There were 813 boys and 991 girls, aged from 13 to 18, with an average age of 16 years (SD = 1.71). Correlational analyses and SPSS macro PROCESS (Model 6) were used for major data analysis. Results indicated that cumulative family risk was positively associated with cyberbullying, and this link could be mediated by school connectedness and cyber victimization. The present study identifies the potential underlying mechanism by which cumulative family risk is associated with adolescent cyberbullying, which has important implications for theory and prevention.
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http://dx.doi.org/10.3389/fpubh.2022.898362DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9271664PMC
June 2022

A cross-sectional study about coronavirus fear of Chinese college students in school lockdowns during the COVID-19 pandemic.

Heliyon 2022 Jul 8;8(7):e09892. Epub 2022 Jul 8.

Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, PR China.

Objective: To remove the phobic psychological situation of Chinese college students who were in school lockdowns during the COVID-19 outbreak, and to provide a theoretical basis for college student psychological intervention.

Methods: Online survey conducted from December 9, 2021, to December 11, 2021. The seven-item Fcv-19s was employed to assess fear of COVID-19 on a Likert five-point scale. Categorical data were reported as number and percentage, whereas continuous data were reported as mean ± standard deviation. Multivariate logistic regression models were used to assess the association between different factors and anxiety symptoms.

Results: The study population consisted of 26.02% (268) male and 73.98% (762) female, of whom approximately 84% were undergraduates. The overall mean score of the questionnaire was 16.04 ± 5.28. Significantly more study populations reported high levels of fear (54.8%), while age, cost of life, professional category, education background (P < 0.05) revealed significant differences based on fear levels.

Conclusions: More than half of the Chinese college students developed fear symptoms during the COVID-19 epidemic. Appropriate psychological interventions for college students should be implemented promptly to reduce the psychological harm induced by the COVID-19 epidemic.
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http://dx.doi.org/10.1016/j.heliyon.2022.e09892DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9263676PMC
July 2022

Does Internet Use Connect Us to a Healthy Diet? Evidence from Rural China.

Authors:
Baojie Ma Xin Jin

Nutrients 2022 Jun 24;14(13). Epub 2022 Jun 24.

China Academy for Rural Development (CARD), Zhejiang University, Hangzhou 310012, China.

Dietary patterns in China have changed dramatically over the past few decades as the Internet has become rapidly available. Based on data from the China Health and Nutrition Survey (2006-2011), we use a two-way fixed effects model and an instrumental variable approach to determine the impact of Internet use on the dietary quality of rural residents. The results indicate that Internet use could significantly improve the dietary quality of Chinese rural residents, with an increase of about 10.4% in the China Food Pagoda Score (CFPS), mainly due to the increase in the dietary quality score for five food groups: fruits, meats, eggs, oil, and salt. We also found that Internet use significantly increased the consumption amounts of milk and its products (4 g), fruits (31 g), eggs (8 g), and vegetables (34 g), while also decreasing the intake of salts (2 g) and oil (6 g). A possible mechanism is that Internet use improves the dietary knowledge of rural residents, thus optimizing their dietary structure. Moreover, the effect of the Internet was greater among females and those who prepare food for a family. Rural residents without a college degree enjoyed more benefits. In summary, governments should further promote Internet penetration in rural areas for health purposes.
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http://dx.doi.org/10.3390/nu14132630DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268265PMC
June 2022

Phrenic artery-pulmonary artery fistula with phrenic artery-pulmonary vein fistula: A rare case report.

Asian J Surg 2022 Jul 5. Epub 2022 Jul 5.

Department of Radiology, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650101, PR China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2022.06.136DOI Listing
July 2022

First-in-human phase I study of CLL-1 CAR-T cells in adults with relapsed/refractory acute myeloid leukemia.

J Hematol Oncol 2022 07 7;15(1):88. Epub 2022 Jul 7.

Tianjin First Central Hospital, The First Affiliated Central Hospital of Nankai University, The First Central Clinical College of Tianjin Medical University, Tianjin, 300192, China.

Relapsed or refractory (R/R) acute myeloid leukemia (AML) has a poor prognosis. In this study, we evaluated chimeric antigen receptor (CAR) T cell therapy targeting CLL-1 in adults with R/R AML patients. Patients received conditioning chemotherapy with cyclophosphamide (500 mg/m) and fludarabine (30 mg/m) for 3 days and an infusion of a dose of 1-2 × 10 CAR-T cells/kg. The incidence of dose-limiting toxicity was the primary endpoint. Ten patients were treated, and all developed cytokine release syndrome (CRS); 4 cases were low-grade, while the remaining 6 were considered high-grade CRS. No patient developed CAR-T cell-related encephalopathy syndrome (CRES). Severe pancytopenia occurred in all patients. Two patients died of severe infection due to chronic agranulocytosis. The complete response (CR)/CR with incomplete hematologic recovery (CRi) rate was 70% (n = 7/10). The median follow-up time was 173 days (15-488), and 6 patients were alive at the end of the last follow-up. CAR-T cells showed peak expansion within 2 weeks. Notably, CLL-1 is also highly expressed in normal granulocytes, so bridging hematopoietic stem cell transplantation (HSCT) may be a viable strategy to rescue long-term agranulocytosis due to off-target toxicity. In conclusion, this study is the first to demonstrate the positive efficacy and tolerable safety of CLL-1 CAR-T cell therapy in adult R/R AML.
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http://dx.doi.org/10.1186/s13045-022-01308-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264641PMC
July 2022

Dynamic response of a coal rock caving impact tail beam for hydraulic support.

Sci Rep 2022 Jul 7;12(1):11535. Epub 2022 Jul 7.

School of Mechanical Engineering, Liaoning Technical University, Fuxin, 123000, China.

Based on the two-way coupling technology of Discrete Element Method-Multi Flexible Body Dynamics (EDM-FMBD), a virtual caving coal wall is established by using the discrete element software, EDEM. The rigid flexible coupling model of the tail beam of caving supports is established by using multibody dynamics software, RecurDyn. The stiffness of the oil cylinder is calculated by using the solid-liquid spring coupling theory and is replaced by a spring. By simulating the process of a coal rock collapse impacting the tail beam, the dynamic signal from the coal rock collapse impacting the tail beam to crushing in the coal caving stage of the comprehensive caving working face is studied, and the test is carried out underground. The angular acceleration at the hinge point of the tail beam is the largest and shows a variation pattern of "large at both ends and small in the middle". The definition of a "low amplitude band" on the surface of the tail beam is proposed. The force signal at the hinge point of the front link is the strongest and is the best measurement point for the force sensor; the angular acceleration signal at the hinge point of the tail beam is the strongest and it is the best measurement point for the angular acceleration sensor. The results have practical implications for the identification of the coal gangue and the adaptive control of support for integrated top coal mining.
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http://dx.doi.org/10.1038/s41598-022-15845-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262896PMC
July 2022
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