Publications by authors named "Xiaoming Zhang"

793 Publications

Single-cell transcriptomic analysis of the tumor ecosystems underlying initiation and progression of papillary thyroid carcinoma.

Nat Commun 2021 Oct 18;12(1):6058. Epub 2021 Oct 18.

Department of Head and Neck Surgery, Fudan University Shanghai Cancer Center, Shanghai, 200032, China.

The tumor ecosystem of papillary thyroid carcinoma (PTC) is poorly characterized. Using single-cell RNA sequencing, we profile transcriptomes of 158,577 cells from 11 patients' paratumors, localized/advanced tumors, initially-treated/recurrent lymph nodes and radioactive iodine (RAI)-refractory distant metastases, covering comprehensive clinical courses of PTC. Our data identifies a "cancer-primed" premalignant thyrocyte population with normal morphology but altered transcriptomes. Along the developmental trajectory, we also discover three phenotypes of malignant thyrocytes (follicular-like, partial-epithelial-mesenchymal-transition-like, dedifferentiation-like), whose composition shapes bulk molecular subtypes, tumor characteristics and RAI responses. Furthermore, we uncover a distinct BRAF-like-B subtype with predominant dedifferentiation-like thyrocytes, enriched cancer-associated fibroblasts, worse prognosis and promising prospect of immunotherapy. Moreover, potential vascular-immune crosstalk in PTC provides theoretical basis for combined anti-angiogenic and immunotherapy. Together, our findings provide insight into the PTC ecosystem that suggests potential prognostic and therapeutic implications.
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http://dx.doi.org/10.1038/s41467-021-26343-3DOI Listing
October 2021

TOF-Based Fast Self-Positioning Algorithm for UWB Mobile Base Stations.

Sensors (Basel) 2021 Sep 23;21(19). Epub 2021 Sep 23.

National Key Laboratory for Electronic Measurement Technology, North University of China, Taiyuan 030051, China.

To solve the problem of heavy workload and high cost when acquiring the position of Ultra-Wideband (UWB) mobile base stations in sports fields, a fast self-positioning algorithm for UWB mobile base stations algorithm based on Time of Flight (TOF) is proposed. First, according to the layout of the base stations in the sports field, the local coordinate system is determined, and an equation based on the ranging information between the base stations is established; the Least Square method is used to calculate the coordinates of each base station, and the Newton Iteration method is used to converge the positioning results. Then the origin and propagation law of positioning error, as well as the method of reducing the positioning error are analyzed. The simulation data and experimental results show that the average positioning accuracy of the mobile base station is within 0.05 m, which meets the expected accuracy of the base station position measurement. Compared with traditional manual measurement methods, base station self-positioning can effectively save deployment time and reduce workload.
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http://dx.doi.org/10.3390/s21196359DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8512438PMC
September 2021

Is Induced by Short Days in Soybean and May Accelerate Flowering in Transgenic .

Int J Mol Sci 2021 Sep 25;22(19). Epub 2021 Sep 25.

Key Laboratory of Soybean Biology of Ministry of Education China, Northeast Agricultural University, Harbin 150030, China.

Flowering is an important developmental process from vegetative to reproductive growth in plant; thus, it is necessary to analyze the genes involved in the regulation of flowering time. The MADS-box transcription factor family exists widely in plants and plays an important role in the regulation of flowering time. However, the molecular mechanism of involved in the regulation of plant flowering is not very clear. In this study, GmFULc protein had a typical MADS domain and it was a member of MADS-box transcription factor family. The expression analysis revealed that was induced by short days (SD) and regulated by the circadian clock. Compared to wild type (WT), overexpression of in transgenic caused significantly earlier flowering time, while mutants flowered later, and overexpression of rescued the late-flowering phenotype of mutants. ChIP-seq of GmFULc binding sites identified potential direct targets, including (), and it inhibited the transcriptional activity of . In addition, the transcription levels of (), () and () in the downstream of were increased in  , suggesting that the early flowering phenotype was associated with up-regulation of these genes. Our results suggested that GmFULc inhibited the transcriptional activity of and induced expression of , and to promote flowering.
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http://dx.doi.org/10.3390/ijms221910333DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8508813PMC
September 2021

GmRAV confers ecological adaptation through photoperiod control of flowering time and maturity in soybean.

Plant Physiol 2021 Sep;187(1):361-377

Key Laboratory of Soybean Biology of Ministry of Education China, Northeast Agricultural University, Harbin 150030, China.

Photoperiod strictly controls vegetative and reproductive growth stages in soybean (Glycine max). A soybean GmRAV (Related to ABI3/VP1) transcription factor containing both AP2 and B3 domains was shown to be a key component of this process. We identified six polymorphisms in the GmRAV promoter that showed significant association with flowering time and maturity of soybean in one or multiple environments. Soybean varieties with minor polymorphism exhibited a longer growth period contributing to soybean adaptation to lower latitudes. The cis-acting element GT1CONSENSUS motif of the GmRAV promoter controlled the growth period, and the major allele in this motif shortened duration of late reproductive stages by reducing GmRAV expression levels. Three GmRAV-overexpressing (GmRAV-ox) transgenic lines displayed later flowering time and maturity, shorter height and fewer numbers of leaves compared with control plants, whereas transgenic inhibition of GmRAV expression resulted in earlier flowering time and maturity and increased plant height. Combining DNA affinity purification sequencing and RNA sequencing analyses revealed 154 putative target genes directly bound and transcriptionally regulated by GmRAV. Two GmRAV binding motifs [C(A/G)AACAA(G/T)A(C/T)A(G/T)] and [C(T/A)A(C)C(T/G)CTG] were identified, and acting downstream of E3E4, GmRAV repressed GmFT5a transcriptional activity through binding a CAACA motif, thereby delaying soybean growth and extending both vegetative and reproductive phases.
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http://dx.doi.org/10.1093/plphys/kiab255DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418415PMC
September 2021

Two-dimensional metallic carbon allotrope with multiple rings for ion batteries.

Phys Chem Chem Phys 2021 Sep 19;23(34):18770-18776. Epub 2021 Aug 19.

State Key Laboratory of Reliability and Intelligence of Electrical Equipment, Hebei University of Technology, Tianjin 300130, China.

Two-dimensional (2-D) materials, especially carbon allotropes, have larger storage capacity and faster diffusion rate due to their unique structures and are usually used in ion batteries. Recently, a new stable two-dimensional carbon allotrope, namely PAI-graphene, was reported by first-principles calculations. Due to its lightweight and multiple-ring structure, great stability and excellent properties, here, we theoretically reveal the excellent performance of PAI-graphene as an anode material for Li-/Na-ion batteries. Our results show that PAI-graphene has intrinsic metallicity before and after adsorption of Li/Na, which ensures that it has good conductivity when working as an electrode material. In addition, PAI-graphene exhibits quite low open circuit voltage (0.342-0.190 V for Li, 0.339-0.233 V for Na) and diffusion barrier (0.34 eV for Li, 0.17 eV for Na), which indicates its superiority as an anode material. Most noteworthily, the Na storage capacity of PAI-graphene is up to 1674 mA h g, which is much higher than that of most 2-D anode materials. Thus, we believe that PAI-graphene can be an outstanding anode material with outstanding performance.
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http://dx.doi.org/10.1039/d1cp02508bDOI Listing
September 2021

Pickering-Droplet-Derived MOF Microreactors for Continuous-Flow Biocatalysis with Size Selectivity.

J Am Chem Soc 2021 Oct 4;143(40):16641-16652. Epub 2021 Oct 4.

School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, People's Republic of China.

Enzymatic microarchitectures with spatially controlled reactivity, engineered molecular sieving ability, favorable interior environment, and industrial productivity show great potential in synthetic protocellular systems and practical biotechnology, but their construction remains a significant challenge. Here, we proposed a Pickering emulsion interface-directed synthesis method to fabricate such a microreactor, in which a robust and defect-free MOF layer was grown around silica emulsifier stabilized droplet surfaces. The compartmentalized interior droplets can provide a biomimetic microenvironment to host free enzymes, while the outer MOF layer secludes active species from the surroundings and endows the microreactor with size-selective permeability. Impressively, the thus-designed enzymatic microreactor exhibited excellent size selectivity and long-term stability, as demonstrated by a 1000 h continuous-flow reaction, while affording completely equal enantioselectivities to the free enzyme counterpart. Moreover, the catalytic efficiency of such enzymatic microreactors was conveniently regulated through engineering of the type or thickness of the outer MOF layer or interior environments for the enzymes, highlighting their superior customized specialties. This study provides new opportunities in designing MOF-based artificial cellular microreactors for practical applications.
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http://dx.doi.org/10.1021/jacs.1c07482DOI Listing
October 2021

Twelve-Month Results From the First-in-China Prospective, Multi-Center, Randomized, Controlled Study of the FREEWAY Paclitaxel-Coated Balloon for Femoropopliteal Treatment.

Front Cardiovasc Med 2021 10;8:686267. Epub 2021 Sep 10.

Department of Interventional Radiology and Vascular Surgery, Peking University First Hospital, Beijing, China.

Several paclitaxel-coated balloons have been proved to provide better efficacy results than uncoated balloons in femoropopliteal lesions. But the efficacy and safety of FREEWAY balloons have not been investigated in Chinese patients. This study aimed to evaluate the efficacy and safety performance of FREEWAY paclitaxel-coated balloons vs. uncoated balloons in Chinese femoropopliteal artery lesions. In this prospective multi-center randomized controlled FREEWAY-CHINA study, 311 patients with symptomatic lower limb ischemia (Rutherford category 2-5) and femoropopliteal lesions of 14 Chinese centers were randomly assigned in a 1:1 ratio to endovascular treatment with either FREEWAY paclitaxel-coated balloons or uncoated balloons (control). The primary endpoint was the 6-month clinically-driven target lesion revascularization (CD-TLR) rate. Secondary endpoints included the device and technical success rate, the ankle-brachial indexes (ABIs), Rutherford category change, the 6-month primary and secondary patency rates, severe adverse effects, and the 12-month CD-TLR rate. The two groups were comparable in terms of their demographic and lesion characteristics. Patients' mean age was 70 years, and 70% were men. The mean lesion length was 71 mm. The 6-month CD-TLR rate was 2.6% in the FREEWAY group and 11.7% in the control group ( = 0.001). The 12-month CD-TLR rate was 2.7% in the FREEWAY group and 13.2% in the control group ( = 0.0005). Other endpoints, including patency rates, major adverse events, and ABI or Rutherford change, did not differ between the two groups. The FREEWAY balloon resulted in an effective decrease in CD-TLR rates and had similar safety results compared to the uncoated balloon in Chinese femoropopliteal artery patients at the 12-month follow-up appointment.
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http://dx.doi.org/10.3389/fcvm.2021.686267DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460758PMC
September 2021

Characteristic flavor formation of thermally processed N-(1-deoxy-α-d-ribulos-1-yl)-glycine: Decisive role of additional amino acids and promotional effect of glyoxal.

Food Chem 2021 Sep 14;371:131137. Epub 2021 Sep 14.

Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick 08901, NJ, USA. Electronic address:

The role of amino acids and α-dicarbonyls in the flavor formation of Amadori rearrangement product (ARP) during thermal processing was investigated. Comparisons of the volatile compounds and their concentrations when N-(1-deoxy-α-d-ribulos-1-yl)-glycine reacted with different amino acids or glyoxal (GO) at 100 °C were executed. Additional amino acids, such as glycine (Gly), in ARP models contributed to the diversity of furanoids by the chain elongation of the derived formaldehyde. Whereas the monoanion of additional glutamic acid acted as nucleophile, favored 2-ethyl-3,5-dimethylpyrazine and 2,5-dimethylpyrazine formation; the nonionized amino group of additional lysine were involved in α-dicarbonyls formation, causing pyrazine and methylpyrazine accumulation in the ARP model. Moreover, the high dosage and pH stabilization of additional GO probably promoted the ARP degradation and deoxyosones retro-aldol cleavage, resulting in methylpyrazine rather than furanoids formation. The present work provided the guidance for the controlled flavor formation of ARP in industrial application.
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http://dx.doi.org/10.1016/j.foodchem.2021.131137DOI Listing
September 2021

Retroperitoneal Recurrence of Melanotic Schwannoma on 18F-FDG PET/CT.

Clin Nucl Med 2021 Sep 22. Epub 2021 Sep 22.

From the Department of Ultrasound Department of Nuclear Medicine, Tangshan Gongren Hospital, Tangshan, Hebei Province, PR China.

Abstract: Melanotic schwannoma is an uncommon nerve sheath neoplasm. Only 10% of melanotic schwannoma are malignant whereas metastases from melanotic schwannoma are much less common. Hereby we present FDG PET/CT findings of recurrent left retroperitoneal melanotic schwannoma with widespread metastases in a 50-year-old man.
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http://dx.doi.org/10.1097/RLU.0000000000003900DOI Listing
September 2021

Current neurologic treatment and emerging therapies in CDKL5 deficiency disorder.

J Neurodev Disord 2021 09 16;13(1):40. Epub 2021 Sep 16.

Division of Epilepsy and Clinical Neurophysiology, Department of Neurology, Boston Children's Hospital, 300 Longwood Avenue, Mailstop 3063, Boston, MA, 02115, USA.

Background: CDKL5 deficiency disorder (CDD) is associated with refractory infantile onset epilepsy, global developmental delay, and variable features that include sleep, behavioral disturbances, and movement disorders. Current treatment is primarily symptom-based and informed by experience in caring for this population.

Methods: We describe medication and non-medication approaches to treatment of epilepsy and additional key neurologic symptoms (sleep disturbances, behavioral issues, movement disorders, and swallowing dysfunction) in a cohort of 177 individuals meeting criteria for CDD, 154 evaluated at 4 CDKL5 Centers of Excellence in the USA and 40 identified through the NIH Natural History Study of Rett and Related Disorders.

Results: The four most frequently prescribed anti-seizure medications were broad spectrum, prescribed in over 50% of individuals. While the goal was not to ascertain efficacy, we obtained data from 86 individuals regarding response to treatment, with 2-week response achieved in 14-48% and sustained 3-month response in 5-36%, of those with known response. Additional treatments for seizures included cannabis derivatives, tried in over one-third of individuals, and clinical trial medications. In combination with pharmacological treatment, 50% of individuals were treated with ketogenic diet for attempted seizure control. Surgical approaches included vagus nerve stimulators, functional hemispherectomy, and corpus callosotomy, but numbers were too limited to assess response. Nearly one-third of individuals received pharmacologic treatment for sleep disturbances, 13% for behavioral dysregulation and movement disorders, and 43% had gastrostomy tubes.

Conclusions: Treatment for neurologic features of CDD is currently symptom-based and empiric rather than CDD-specific, though clinical trials for CDD are emerging. Epilepsy in this population is highly refractory, and no specific anti-seizure medication was associated with improved seizure control. Ketogenic diet is commonly used in patients with CDD. While behavioral interventions are commonly instituted, information on the use of medications for sleep, behavioral management, and movement disorders is sparse and would benefit from further characterization and optimization of treatment approaches. The heterogeneity in treatment approaches highlights the need for systematic review and guidelines for CDD. Additional disease-specific and disease-modifying treatments are in development.
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http://dx.doi.org/10.1186/s11689-021-09384-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8447578PMC
September 2021

Two-Stage Calibration Scheme for Magnetic Measurement System on Guided Munition.

Sensors (Basel) 2021 Aug 28;21(17). Epub 2021 Aug 28.

National Key Laboratory for Electronic Measurement Technology, North University of China, Taiyuan 030051, China.

In order to calibrate the magnetic measurement system used in guided munition on site, a two-stage calibration (TSC) scheme without reference is proposed in this paper. Analyzing the interfering magnetic field in the projectile and misalignment angles between the projectile coordinate system and measurement coordinate system establishes a proper mathematical equivalent model and derives a calibration method. The first stage is ellipsoid fitting to obtain the equivalent zero-offset, equivalent sensitivity and equivalent non-orthogonal angles of the sensor; the second stage is to calibrate the misalignment angles between the projectile coordinate system and the measurement coordinate system with the three-position calibration (TPC) method. Complete calibration is convenient to operate and does not need an additional reference, which has wide applicability. The simulation results show that the deviation in the measured value after compensation is within 100 nT. The experiment proves that the error of compensated magnetic value is about 150 nT, which meets the accuracy of requirements in guided munitions.
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http://dx.doi.org/10.3390/s21175799DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8434620PMC
August 2021

Determining the Phase Separation Characteristics of Plant Proteins.

Curr Protoc 2021 Sep;1(9):e237

State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Eukaryotic cells contain numerous membrane-bound and membraneless organelles that provide spatiotemporal control for diverse biological processes. The liquid-liquid phase separation of proteins has been proposed as the driving force behind the formation of membraneless organelles. Here, we describe a method to determine the phase separation activities of proteins in plants. This basic method includes protocols for an in vivo fluorescence recovery after photobleaching assay in Nicotiana benthamiana using transient expression, an in vitro liquid droplet reconstitution assay using purified recombinant proteins, and an in vivo fluorescence recovery after photobleaching assay in Arabidopsis thaliana using stable transgenic plants. With these assays, the phase separation characteristics of a protein can be determined. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Detection of protein phase separation activities in N. benthamiana Support Protocol: Fluorescence recovery after photobleaching assay Basic Protocol 2: Detection of protein phase separation in vitro Basic Protocol 3: Detection of protein phase separation in stable transgenic Arabidopsis thaliana plants.
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http://dx.doi.org/10.1002/cpz1.237DOI Listing
September 2021

Comparing the Performance of the ABC, AIMS65, GBS, and pRS Scores in Predicting 90-day Mortality Or Rebleeding Among Emergency Department Patients with Acute Upper Gastrointestinal Bleeding: A Prospective Multicenter Study.

J Transl Int Med 2021 Jun 16;9(2):114-122. Epub 2021 Jun 16.

Department of Emergency Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Science, Beijing, China.

Background And Objectives: Acute upper gastrointestinal bleeding (UGIB) is a common problem that can cause significant morbidity and mortality. We aimed to compare the performance of the ABC score (ABC), the AIMS65 score (AIMS65), the Glasgow-Blatchford score (GBS), and the pre-endoscopic Rockall score (pRS) in predicting 90-day mortality or rebleeding among patients with acute UGIB.

Methods: This was a prospective multicenter study conducted at 20 tertiary hospitals in China. Data were collected between June 30, 2020 and February 10, 2021. An area under the receiver operating characteristic curve (AUC) analysis was used to compare the performance of the four scores in predicting 90-day mortality or rebleeding.

Results: Among the 1072 patients included during the study period, the overall 90-day mortality rate was 10.91% (117/1072) and the rebleeding rate was 12.03% (129/1072). In predicting 90-day mortality, the ABC and pRS scores performed better with an AUC of 0.722 (95% CI 0.675-0.768; <0.001) and 0.711 (95% CI 0.663-0.757; <0.001), respectively, compared to the AIMS-65 (AUC, 0.672; 95% CI, 0.624-0.721; <0.001) and GBS (AUC, 0.624; 95% CI, 0.569-0.679; <0.001) scores. In predicting rebleeding in 90 days, the AUC of all scores did not exceed 0.70.

Conclusion: In patients with acute UGIB, ABC and pRS performed better than AIMS-65 and GBS in predicting 90-day mortality. The performance of each score is not satisfactory in predicting rebleeding, however. Newer predictive models are needed to predict rebleeding after UGIB.
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http://dx.doi.org/10.2478/jtim-2021-0026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386323PMC
June 2021

Degradation of 2-Threityl-Thiazolidine-4-Carboxylic Acid and Corresponding Browning Accelerated by Trapping Reaction between Extra-Added Xylose and Released Cysteine during Maillard Reaction.

J Agric Food Chem 2021 Sep 31;69(36):10648-10656. Epub 2021 Aug 31.

Anhui Province Key Laboratory of Functional Compound Seasoning, Anhui Qiangwang Flavouring Food Co., LTD, No. 1 Shengli Road, Jieshou, Anhui 236500, P. R. China.

2-Threityl-thiazolidine-4-carboxylic acid (TTCA), a nonvolatile precursor of flavor and color, is considered to be more stable than its isomeric Amadori compound (ARP). The degradation behavior of TTCA favors higher temperatures and pH. In order to adjust and control the thermal degradation of TTCA to improve its food processing adaptability, a TTCA-Xyl thermal reaction model was constructed to explore the effect of extra-added Xyl on the thermal degradation behavior of TTCA. The results confirmed that the extra-added Xyl was involved in the degradation pathway of TTCA and accelerated its depletion, thus promoting the formation of characteristic downstream products of TTCA including some α-dicarbonyl compounds, and consequently accelerating the browning formation. The isotope-labeling technique was further applied to confirm that the added Xyl could trap the Cys released from the decomposition of ARP and formed additional TTCA, which could promote the movement of chemical equilibrium and gradually accelerate the degradation rate of TTCA as well as melanoidins formation. The higher pH value could even promote this phenomenon.
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http://dx.doi.org/10.1021/acs.jafc.1c03727DOI Listing
September 2021

Maillard Browning Inhibition by Ellagic Acid via Its Adduct Formation with the Amadori Rearrangement Product.

J Agric Food Chem 2021 Sep 24;69(34):9924-9933. Epub 2021 Aug 24.

Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, New Jersey 08901, United States.

The Maillard reaction performed under a stepwise increase of temperature was applied for researching the inhibition of Maillard browning caused by ellagic acid. Ellagic acid was found effective for the inhibition of melanoidin formation in the xylose-glycine Maillard reaction but depended on its dosage and the point of time it was added in the reaction system. The lightest color of the Maillard reaction products was observed when ellagic acid was added at the 90th min, which was the point of time when the Amadori rearrangement product (ARP) developed the most. LC-ESI-MS/MS analysis results showed a significant tendency of the ellagic acid hydrolysis product to react with the predominant intermediate ARP to yield an adduct. The adduct stabilized the ARP and delayed its decomposition and inhibited the downstream reactions toward browning. After the ARP was depleted, ellagic acid also showed an effect on scavenging some short-chain dicarbonyls which contributed to the inhibition of Maillard browning.
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http://dx.doi.org/10.1021/acs.jafc.1c03481DOI Listing
September 2021

Spatiotemporal Immune Landscape of Colorectal Cancer Liver Metastasis at Single-Cell Level.

Cancer Discov 2021 Aug 20. Epub 2021 Aug 20.

Depart. of Liver Surgery and Transplantation, Liver Cancer Institute, Zhong Shan Hospital and Shanghai Medical School, Fudan University,

Liver metastasis, the leading cause of colorectal cancer mortality, exhibits a highly heterogeneous and suppressive immune microenvironment. Here, we sequenced 97 matched samples by using single-cell RNA-seq and Spatial Transcriptomics. Strikingly, metastatic microenvironment underwent remarkable spatial reprogramming of immunosuppressive cells such as MRC1+ CCL18+ M2-like macrophages. We further developed scMetabolism, a computational pipeline for quantifying single-cell metabolism, and observed that those macrophages harbored enhanced metabolic activity. Interestingly, neoadjuvant chemotherapy could block this status and restore the antitumor immune balance in responsive patients, while the non-responsive patients deteriorated into a more suppressive one. Our work described the immune evolution of metastasis and uncovered the black box of how tumors respond to neoadjuvant chemotherapy.
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http://dx.doi.org/10.1158/2159-8290.CD-21-0316DOI Listing
August 2021

Correction: LncRNA BCYRN1 inhibits glioma tumorigenesis by competitively binding with miR-619-5p to regulate CUEDC2 expression and the PTEN/AKT/p21 pathway.

Oncogene 2021 Oct;40(40):5972-5973

Department of Neurosurgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230001, Anhui, P.R. China.

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http://dx.doi.org/10.1038/s41388-021-01990-4DOI Listing
October 2021

TP53 Mutation and Extraneural Metastasis of Glioblastoma: Insights From an Institutional Experience and Comprehensive Literature Review.

Am J Surg Pathol 2021 11;45(11):1516-1526

Departments of Pathology and Laboratory Medicine.

Extraneural metastases of glioblastoma (GBM), although rare, are becoming an increasingly recognized occurrence. Currently, the biological mechanism underlying this rare occurrence is not understood. To explore the potential genomic drivers of extraneural metastasis in GBM, we present the molecular features of 4 extraneural metastatic GBMs, along with a comprehensive review and analysis of previously reported cases that had available molecular characterization. In addition to our 4 cases, 42 patients from 35 publications are reviewed. To compare the molecular profiles between GBM cases with extraneural metastasis and the general GBM population, genomic data from GBM samples in The Cancer Genome Atlas (TCGA) database were also analyzed. We found that 64.5% (20/31) of the cases with extraneural metastasis that were tested for TP53 changes had at least 1 TP53 pathogenic variant detected in either 1 or both primary and metastatic tumors. In contrast, TP53 mutation was significantly less frequent in the unselected GBM from TCGA (22.6%, 56/248) (P=0.000). In addition, O-6-methylguanine-DNA methyltransferase (MGMT) promoter methylation was more common in unselected TCGA GBM cases (48.6%, 170/350) than in cases with extraneural metastasis (31.8%, 7/22), although not statistically significant. Although isocitrate dehydrogenase (IDH) mutation is a rare occurrence in high-grade astrocytomas, IDH-mutant grade 4 astrocytomas are at least as likely to metastasize as IDH wild-type GBMs; 3 metastatic cases definitively harbored an IDH1 (p.R132H) mutation in our analysis. Our findings not only provide potential biomarkers for earlier screening of extraneural metastasis, but could also suggest clues to understanding biological mechanisms underlying GBM metastasis, and for the development of therapeutic modalities.
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http://dx.doi.org/10.1097/PAS.0000000000001762DOI Listing
November 2021

In Situ Chemical Lithiation Transforms Diamond-Like Carbon into an Ultrastrong Ion Conductor for Dendrite-Free Lithium-Metal Anodes.

Adv Mater 2021 Sep 31;33(37):e2100793. Epub 2021 Jul 31.

Functional Thin Films Research Center, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.

Lithium (Li)-metal anodes are of great promise for next-generation batteries due to their high theoretical capacity and low redox potential. However, Li-dendrite growth during cycling imposes a tremendous safety concern on the practical application of Li-metal anodes. Herein, an effective approach to suppress Li-dendrite growth by coating a polypropylene (PP) separator with a thin layer of ultrastrong diamond-like carbon (DLC) is reported. Theoretical calculations indicate that the DLC coating layer undergoes in situ chemical lithiation once assembled with the lithium-metal anode, transforming the DLC/PP separator into an excellent 3D Li-ion conductor. This in situ lithiated DLC/PP separator can not only mechanically suppress Li-dendrite growth by its intrinsically high modulus (≈100 GPa), but also uniformly redistributes Li ions to render dendrite-free lithium deposition. The twofold effects of the DLC/PP separator result in stable cycling of lithium plating/stripping (over 4500 h) at a high current density of 3 mA cm . Remarkably, this approach enables more than 1000 stable cycles at 5 C with a capacity retention of ≈71% in a Li || LiFePO coin cell and more than 200 stable cycles at 0.2 C in a Li || LiNi Co Mn O pouch cell with cathode mass loading of ≈9 mg cm .
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http://dx.doi.org/10.1002/adma.202100793DOI Listing
September 2021

Characteristics, kinetics, thermodynamics and long-term effects of zerovalent iron/pyrite in remediation of Cr(VI)-contaminated soil.

Environ Pollut 2021 Nov 23;289:117830. Epub 2021 Jul 23.

School of Metallurgy and Environment, Central South University, Changsha, 410083, PR China; Chinese National Engineering Research Centre for Control & Treatment of Heavy Metal Pollution, Changsha, 410083, PR China. Electronic address:

Development of efficient, green and low-cost natural mineral-based reductive materials is promising to remediation of hexavalent chromium(Cr(VI))-contaminated soil. Considering the synergetic effect between pyrite and zerovalent iron (ZVI), an activated pyrite supported ZVI(ZVI/FeS) with high reducing activity was developed by ball milling activation of natural pyrite and sulfidation of ZVI. The remediation property of ZVI/FeS for Cr(VI)-contaminated soil was evaluated with different ZVI/FeS dosage, soil-water ratio, initial pH, time and temperature, as well as the stability of Cr. The results showed that ZVI/FeS possessed high reduction activity with soil Cr(VI) removal rate up to 99 % even under alkaline condition, and soil with different pH values eventually converged to neutral after 90 days, indicating that ZVI/FeS has a good self-regulating alkaline ability. The reduction process conformed to Langmuir-Hinshelwood first-order kinetics and was a spontaneous and endothermic process. The lower activation energy of 17.97 kJ mol (usually 60-250 kJ mol) indicated that the reduction reaction of Cr(VI) was particularly easy to occur. The speciation change of Cr in soil within 30 days demonstrated that the Cr in the soil was converted from a readily migratable state to a more stable state, where the Fe-Mn oxide bound fraction reached 85.03 % due to the generation of Cr(III)/Fe(III) co-precipitation. The results of long-term stability experiments showed that the leaching concentrations of Cr(VI) and total Cr decreased significantly after the ZVI/FeS treatment and remained stable at very low levels for 180 days. This study provided a sustainable way to fully utilize natural pyrite minerals to obtain iron-bearing reductive materials for feasible, effective and long-term stable immobilization of Cr(VI) in soil.
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http://dx.doi.org/10.1016/j.envpol.2021.117830DOI Listing
November 2021

Surface Magnetism in Pristine α Rhombohedral Boron and Intersurface Exchange Coupling Mechanism of Boron Icosahedra.

J Phys Chem Lett 2021 Jul 16;12(29):6812-6817. Epub 2021 Jul 16.

Department of Electronics, College of Electronic Information and Optical Engineering, Nankai University, Tianjin 300071, China.

We report intrinsic surface magnetism in pristine α rhombohedral boron (α-boron) using first-principles calculations. Semiconducting α-boron has been cleaved along the (001), (102̅), and (101) planes to produce icosahedral-based non-van der Waals face-boron, t-face-boron, and edge-boron structures, respectively. Face-boron is found to be metallic, while t-face-boron and edge-boron show semiconducting features. In particular, edge-boron exhibits layer-dependent magnetism with a transition from an overall antiferromagnetic (AFM) state with AFM surfaces to either an AFM or a ferromagnetic (FM) state with FM surfaces as the number of layers increases. The magnetism in edge-boron arises from the spin polarization of boron atoms with unsaturated bonds at the edge sites in the upper and lower surfaces, and magnetic exchange coupling can be mediated via adjacent boron icosahedra by up to a maximum of 8.4 Å. These findings deepen our understanding of icosahedral-based boron and boron-rich materials, which may be useful in potential spintronics applications.
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http://dx.doi.org/10.1021/acs.jpclett.1c01860DOI Listing
July 2021

Significant optical force enhancements of nanostructure with balanced gain and loss.

Opt Express 2021 Jul;29(14):22202-22213

In this paper, we theoretically analyze the optical force between a pair of active and passive plasmonic core-shell nanoparticles (NPs). The optical force between the NPs can be either attractive or repulsive near the critical point while the passive dimer provides only attractive force. We reveal that the reversal of attractive or repulsive force is determined by the relative phase of electric dipole (ED) modes, which can be strongly affected by the gain and loss coefficient κ. Compared with the passive dimer with the same size, the active-passive dimer can exhibit a very high repulsive force (about two orders of magnitude) while remaining the same order magnitude attractive force when the value of coefficient is 0.345. Interestingly, we find that the position of the maximum repulsive force occurs near the critical point. We also investigate the influence of variations in geometrical parameters of the dimer and polarization angle on the force. Finally, the numerical results demonstrate that when the dimer is illuminated by a laser beam, the attractive and repulsive forces can also be achieved. The manipulation of optical force can find potential in optical sorting and transport of NPs.
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http://dx.doi.org/10.1364/OE.430719DOI Listing
July 2021

Differences and Similarities of Photocatalysis and Electrocatalysis in Two-Dimensional Nanomaterials: Strategies, Traps, Applications and Challenges.

Nanomicro Lett 2021 Jul 15;13(1):156. Epub 2021 Jul 15.

Beijing Key Laboratory of Micro-Nano Energy and Sensor, CAS Center for Excellence in Nanoscience, Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing, 101400, People's Republic of China.

Photocatalysis and electrocatalysis have been essential parts of electrochemical processes for over half a century. Recent progress in the controllable synthesis of 2D nanomaterials has exhibited enhanced catalytic performance compared to bulk materials. This has led to significant interest in the exploitation of 2D nanomaterials for catalysis. There have been a variety of excellent reviews on 2D nanomaterials for catalysis, but related issues of differences and similarities between photocatalysis and electrocatalysis in 2D nanomaterials are still vacant. Here, we provide a comprehensive overview on the differences and similarities of photocatalysis and electrocatalysis in the latest 2D nanomaterials. Strategies and traps for performance enhancement of 2D nanocatalysts are highlighted, which point out the differences and similarities of series issues for photocatalysis and electrocatalysis. In addition, 2D nanocatalysts and their catalytic applications are discussed. Finally, opportunities, challenges and development directions for 2D nanocatalysts are described. The intention of this review is to inspire and direct interest in this research realm for the creation of future 2D nanomaterials for photocatalysis and electrocatalysis.
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http://dx.doi.org/10.1007/s40820-021-00681-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282827PMC
July 2021

6-lncRNA Assessment Model for Monitoring and Prognosis of HER2-Positive Breast Cancer: Based on Transcriptome Data.

Pathol Oncol Res 2021 13;27:609083. Epub 2021 Apr 13.

College of Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, China.

In view of the high malignancy and poor prognosis of human epidermal growth factor receptor 2 (HER2)-positive breast cancer, we analyzed the RNA expression profiles of HER2-positive breast cancer samples to identify the new prognostic biomarkers. The linear fitting method was used to identify the differentially expressed RNAs from the HER2-positive breast cancer RNA expression profiles in the Cancer Genome Atlas (TCGA). Then, a series of methods including univariate Cox, Kaplan-Meier, and random forests, were used to identify the core long non-coding RNAs (lncRNAs) with stable prognostic value for HER2-positive breast cancer. A clinical feature analysis was performed, and a competing endogenous RNA network was constructed to explore the role of these core lncRNAs in HER2-positive breast cancer. In addition, a functional analysis of differentially expressed messenger RNAs in HER-2 positive breast cancer also provided us with some enlightening insights. The high expression of four core lncRNAs (AC010595.1, AC046168.1, AC069277.1, and AP000904.1) was associated with worse overall survival, while the low expression of LINC00528 and MIR762HG was associated with worse overall survival. The 6-lncRNA model has an especially good predictive power for overall survival ( < 0.0001) and 3-year survival (the area under the curve = 0.980) in HER2-positive breast cancer patients. This study provides a new efficient prognostic model and biomarkers of HER2-positive breast cancer. Meanwhile, it also provides a new perspective for elucidating the molecular mechanisms underlying HER2-positive breast cancer.
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http://dx.doi.org/10.3389/pore.2021.609083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262145PMC
April 2021

Reference ranges of fetal heart function using a Modified Myocardial Performance Index: a prospective multicentre, cross-sectional study.

BMJ Open 2021 07 7;11(7):e049640. Epub 2021 Jul 7.

Department of Ultrasound, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China

Objectives: The primary aim of this study was to establish the normal reference ranges of the fetal left ventricular (LV) Modified Myocardial Performance Index (Mod-MPI). A secondary aim was to evaluate the agreement between manual and automatic measurements for fetal Mod-MPI.

Design: A prospective, multicentre, cross-sectional study.

Participants: Normal singleton pregnancies.

Methods: The LV functions of normal singleton pregnancies were assessed in nine centres covering eight provinces in China using unified ultrasound protocols and settings and standardised measurements by pulsed Doppler at 20-24, 28-32 and 34-38 weeks of gestation. The isovolumetric relaxation time (IRT), isovolumetric contraction time, ejection time (ET) and Mod-MPI were measured both automatically and manually.

Results: This cross-sectional study included 2081 fetuses, and there was a linear correlation between gestational age (GA) and Mod-MPI (0.416+0.001×GA (weeks), p<0.001, r=0.013), IRT (36.201+0.162× GA (weeks), p<0.001, r=0.021) and ET (171.418-0.078*GA (weeks), p<0.001, r=0.002). This finding was verified using longitudinal data in a subgroup of 610 women. The agreement between the manual and automated measurements for Mod-MPI was good.

Conclusions: We constructed normal reference values of fetal LV Mod-MPI. Automatic measurement can be considered for ease of measurement in view of the good agreement between the automatic and manual values.
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http://dx.doi.org/10.1136/bmjopen-2021-049640DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264880PMC
July 2021

Iron-Catalyzed Diborylation of Unactivated Aliphatic -Dihalogenoalkenes: Synthesis of 1,2-Bis(boryl)alkanes.

Org Lett 2021 Jul 7;23(14):5565-5570. Epub 2021 Jul 7.

Sichuan Key Laboratory of Medical Imaging, North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.

Herein, we report the first example of iron-catalyzed defluoroborylation of unactivated -difluoroalkenes, -dichloroalkenes, and -dibromoalkenes, providing the 1,2-bis(boryl)alkanes in moderate to good yield. This transformation has high regioselectivity, wide substrate scope, and excellent functional group compatibility. Preliminary mechanistic studies indicate that double β-F elimination is involved in the catalytic cycle, and the 1,1-diborylated alkenes might be intermediates in this iron-catalyzed 1,2-diborylation reaction.
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http://dx.doi.org/10.1021/acs.orglett.1c01967DOI Listing
July 2021

Depressive State in the Emergency Department During COVID-19: A National Cross-Sectional Survey in China.

Front Psychiatry 2021 14;12:566990. Epub 2021 Jun 14.

Department of Emergency Medicine, The First Affiliate Hospital of Xinjiang Medical University, Wulumuqi, China.

Chinese emergency department (ED) staff encountered significant mental stress while fighting the coronavirus disease 2019 (COVID-19) pandemic. We sought to investigate the prevalence and associated factors for depressive symptoms among ED staff (including physicians, nurses, allied health, and auxiliary ED staff). A cross-sectional national survey of ED staff who were on duty and participated in combating the COVID-19 pandemic was conducted March 1-15, 2020. A total of 6,588 emergency medical personnel from 1,060 hospitals responded to this survey. A majority of respondents scored above 10 points on the PHQ-9 standardized test, which is associated with depressive symptoms. Those aged 31-45, those working in the COVID-19 isolation unit, and those with relatives ≤ 16 or ≥70 years old at home all had statistically significant associations with scoring >10 points. Depressive symptoms among Chinese emergency medical staff were likely quite common during the response to the COVID-19 pandemic and reinforce the importance of targeted ED staff support during future outbreaks.
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http://dx.doi.org/10.3389/fpsyt.2021.566990DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8236535PMC
June 2021

Yolk-Shell Nanocapsule Catalysts as Nanoreactors with Various Shell Structures and Their Diffusion Effect on the CO Reforming of Methane.

ACS Appl Mater Interfaces 2021 Jul 30;13(27):31699-31709. Epub 2021 Jun 30.

Engineering Research Center of the Ministry of Education for Fine Chemicals, Shanxi University, Taiyuan 030006, P. R. China.

Well-geometric-confined yolk-shell catalysts can act as nanoreactors that are of benefit for the antisintering of metals and resistance to coke formation in high-temperature reactions such as the CO reforming of methane. Notwithstanding the credible advances of core/yolk-shell catalysts, the enlarged shell diffusion effects that occur under high space velocity can deactivate the catalysts and hence pose a hurdle for the potential application of these types of catalysts. Here, we demonstrated the importance of the shell thickness and porosity of small-sized [email protected] nanoreactor catalysts, which can vary the diffusional paths/rates of the diffusants that directly affect the catalytic activity. The nanoreactor with an ∼4.5 nm shell thickness and rich pores performed the best in tolerating the shell diffusion effects, and importantly, no catalytic deactivation was observed. We further proposed a shell diffusion effect scheme by modifying the Weisz-Prater and blocker model and found that the "gas wall/hard blocker" formed on the openings of the shell pores can cause reversible/irreversible interruption of the shell mass transfer and thus temporarily/permanently deactivate the nanoreactor catalysts. This work highlights the shell diffusion effects, apart from the metal sintering and coke formation, as an important factor that are ascribed to the deactivation of a nanoreactor catalyst.
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http://dx.doi.org/10.1021/acsami.1c06847DOI Listing
July 2021

Plasma Kidney Injury Molecule 1 in CKD: Findings From the Boston Kidney Biopsy Cohort and CRIC Studies.

Am J Kidney Dis 2021 Jun 24. Epub 2021 Jun 24.

Section of Nephrology, Department of Medicine, Boston University School of Medicine, Boston Medical Center, Boston, MA; Renal Division, Brigham and Women's Hospital, Harvard Medical School, Boston, MA. Electronic address:

Rationale & Objective: Plasma kidney injury molecule-1 (KIM-1) is a sensitive marker of proximal tubule injury, but its association with risks of adverse clinical outcomes across a spectrum of kidney diseases is unknown.

Study Design: Prospective, observational cohort study.

Setting & Participants: 524 individuals undergoing clinically indicated native kidney biopsy with biopsy specimens adjudicated for semiquantitative scores of histopathology by two kidney pathologists enrolled into the Boston Kidney Biopsy Cohort (BKBC) Study and 3,800 individuals with common forms of chronic kidney disease (CKD) enrolled into the Chronic Renal Insufficiency Cohort (CRIC) Study.

Exposure: Histopathologic lesions and clinicopathologic diagnosis in cross-sectional analyses, baseline plasma KIM-1 in prospective analyses.

Outcomes: Baseline plasma KIM-1 in cross-sectional analyses, kidney failure (defined as initiation of kidney replacement therapy) and death in prospective analyses.

Analytical Approach: Multivariable-adjusted linear regression models tested associations of plasma KIM-1 with histopathologic lesions and clinicopathologic diagnoses. Cox proportional hazards models tested associations of plasma KIM-1 with future kidney failure and death.

Results: In the BKBC Study, higher plasma KIM-levels were associated with more severe acute tubular injury, tubulointerstitial inflammation, and more severe mesangial expansion after multivariable adjustment. Participants with diabetic nephropathy, glomerulopathies, and tubulointerstitial disease had significantly higher plasma KIM-1 levels after multivariable adjustment. In the BKBC Study, 124 participants progressed to kidney failure and 85 participants died during a median follow-up time of 5 years. In the CRIC Study, 1153 participants progressed to kidney failure and 1356 participants died during a median follow-up time of 11.5 years. In both cohorts, each doubling of plasma KIM-1 was associated with an increased risk of kidney failure after multivariable adjustment (BKBC: HR 1.19, 95% CI 1.03 to 1.38 and CRIC: HR 1.10, 95% CI 1.06 to 1.15). There was no statistically significant association of plasma KIM-1 with death in either cohort.

Limitations: Generalizability and unmeasured confounding.

Conclusions: Plasma KIM-1 is associated with underlying tubulointerstitial and mesangial lesions and progression to kidney failure in two cohort studies of individuals with kidney diseases.
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http://dx.doi.org/10.1053/j.ajkd.2021.05.013DOI Listing
June 2021

CircRNA HIPK3 promotes the progression of oral squamous cell carcinoma through upregulation of the NUPR1/PI3K/AKT pathway by sponging miR-637.

Ann Transl Med 2021 May;9(10):860

School of Dentistry, Binzhou Medical University, Binzhou, China.

Background: To investigate the expression, function, and related mechanisms of circHIPK3 in oral squamous cell carcinoma (OSCC).

Methods: CircHIPK3 expression was determined by quantitative reverse transcription polymerized chain reaction (QRT-PCR) in OSCC and adjacent tissues, and the correlation between the circHIPK3 level and clinicopathological indexes of OSCC was analyzed. CircHIPK3 expressions in different OSCC cell lines were detected, cell counting kit-8 (CCK-8) and 5-bromodeoxyuridine (BrdU) assays were utilized to monitor cell proliferation and activity. Flow cytometry was adopted to detect apoptosis and transwell assay was used to detect cell invasion. The expressions of nuclear protein 1 (NUPR1), phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) (PI3K/AKT) pathway proteins, and E-cadherin, Vimentin, and N-cadherin markers of epithelial-mesenchymal transformation (EMT) were detected by Western blot or Quantitative Real-time PCR (QRT-PCR).

Results: Upregulated circHIPK3 was noted in OSCC tissues (compared with adjacent tissues), and its overexpression was related to OSCC size and histopathological grade. Functionally, overexpressed circHIPK3 can significantly promote EMT, proliferation, and invasion of OSCC cells and can inhibit cell apoptosis and . In addition, CircHIPK3 upregulated the activation of NUPR1 and PI3K/AKT. Bioinformatics analyses showed that miR-637 was the common target of circHIPK3 and NUPR1, while a dual luciferase reporting assay and RIP assay further demonstrated that circHIPK3 targeted miR-637 and bound to 3' UTR of NUPR1.

Conclusions: CircHIPK3 demonstrates potential as a prognostic marker of OSCC and mediates OSCC progression via the miR-637-mediated NUPR1/PI3K/AKT axis.
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http://dx.doi.org/10.21037/atm-21-1908DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184441PMC
May 2021
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