Publications by authors named "Xiaofang Li"

334 Publications

Immunomodulatory effect of psoriasis-derived dermal mesenchymal stem cells on TH1/TH17 cells.

Eur J Dermatol 2021 Jun;31(3):318-325

Shanxi Key Laboratory of Stem Cells for Immunological Dermatosis, Institute of Dermatology, Taiyuan Central Hospital of Shanxi Medical University, No. 5 Dong San Dao Xiang, Jiefang Road, Taiyuan 030009, China.

T cell-mediated inflammation plays an important role in the development of psoriasis. Mesenchymal stem cells (MSCs) are a population of multipotent cells that regulate the T cell-mediated immune response. To investigate the effects of psoriatic dermal mesenchymal stem cells (p-DMSCs) on proliferation, apoptosis and differentiation of T cells. p-DMSCs and normal DMSCs (n-DMSCs) were isolated from psoriatic skin and normal healthy controls, respectively, and co-cultured with activated T cells isolated from healthy volunteers using a Transwell system. Proliferation and apoptosis of T cells were assessed by cell count and flow cytometry, respectively. Expression levels of transcription factors associated with subtypes of T cells and cytokines were measured by qRT-PCR and western blot. Both p-DMSCs and n-DMSCs inhibited T cell proliferation and cytokine production. Similarly, the presence of p-DMSCs and n-DMSCs decreased the expression levels of both T-bet and ROR-γt in T cells. However, n-DMSCs exhibited a stronger inhibitory effect than p-DMSCs on T cell proliferation, cytokine production, and T-bet and ROR-γt expression. These results suggest that the effect of p-DMSCs on T cell function could contribute, at least in part, to the pathogenesis of psoriasis.
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http://dx.doi.org/10.1684/ejd.2021.4050DOI Listing
June 2021

Atractylenolides (I, II, and III): a review of their pharmacology and pharmacokinetics.

Arch Pharm Res 2021 Jul 16;44(7):633-654. Epub 2021 Jul 16.

School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, P. R. China.

Atractylodes macrocephala Koidz is a widely used as a traditional Chinese medicine. Atractylenolides (-I, -II, and -III) are a class of lactone compounds derived from Atractylodes macrocephala Koidz. Research into atractylenolides over the past two decades has shown that atractylenolides have anti-cancer, anti-inflammatory, anti-platelet, anti-osteoporosis, and antibacterial activity; protect the nervous system; and regulate blood glucose and lipids. Because of structural differences, both atractylenolide-I and atractylenolide-II have remarkable anti-cancer activities, and atractylenolide-I and atractylenolide-III have remarkable anti-inflammatory and neuroprotective activities. We therefore recommend further clinical research on the anti-cancer, anti-inflammatory and neuroprotective effects of atractylenolides, determine their therapeutic effects, alone or in combination. To investigate their ability to regulate blood glucose and lipid, as well as their anti-platelet, anti-osteoporosis, and antibacterial activities, both in vitro and in vivo studies are necessary. Atractylenolides are rapidly absorbed but slowly metabolized; thus, solubilization studies may not be necessary. However, due to the inhibitory effects of atractylenolides on metabolic enzymes, it is necessary to pay attention to the possible side effects of combining atractylenolides with other drugs, in clinical application. In short, atractylenolides have considerable medicinal value and warrant further study.
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http://dx.doi.org/10.1007/s12272-021-01342-6DOI Listing
July 2021

Therapeutic potential of catalpol and geniposide in Alzheimer's and Parkinson's diseases: A snapshot of their underlying mechanisms.

Brain Res Bull 2021 Jul 1;174:281-295. Epub 2021 Jul 1.

School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China. Electronic address:

Rehmannia glutinosa, the fresh or dried root of Rehmannia glutinosa (Gaertn.) Libosch. ex Fisch. & Mey., and Gardenia, the fruit of Gardenia jasminoides Ellis from Rubiaceae, both are famous traditional Chinese medicines that have been traditionally used in China. Catalpol and geniposide, as two kinds of iridoid glycosides with high activities, are the main bioactive components in Rehmannia glutinosa and Gardenia jasminoides Ellis, respectively. Over the past few decades, catalpol and geniposide have been widely studied for their therapeutic effects. The preclinical experiments demonstrated that they possessed significant neuroprotective activities against Alzheimer's disease, Parkinson's disease, stroke, and depression, etc. In this paper, the pharmacological effects and mechanisms of catalpol and geniposide on Alzheimer's disease and Parkinson's disease from 2005 to now were systematically summarized and comprehensively analyzed. At the same time, the pharmacokinetic characteristics of the analyzed compounds were also described, hoping to provide some enlightenment for the design, research, and development of iridoid glycosides.
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http://dx.doi.org/10.1016/j.brainresbull.2021.06.020DOI Listing
July 2021

Efficient visible light photocatalytic NO abatement over SrSn(OH) nanowires loaded with Ag/AgO cocatalyst.

Environ Res 2021 Jun 29;201:111521. Epub 2021 Jun 29.

Chongqing Key Laboratory of Catalysis and New Environmental Materials, College of Environment and Resources, National Research Base of Intelligent Manufacturing Service, Chongqing Technology and Business University, Chongqing, 400067, China; Yangtze Delta Region Institute (Huzhou) & School of Resources and Environment, University of Electronic Science and Technology of China, Huzhou, 313001, China. Electronic address:

SrSn(OH) (SSOH) possesses a high oxidation potential in the valence band (VB), which is suitable for photocatalytic oxidation removal of pollutants. However, the electrons in the VB of these catalysts are difficult to transition to the conduction band (CB) under visible light, which makes it difficult to utilize sunlight effectively. In this work, Ag/AgO is loaded on the surface of SSOH nanowires, which stimulates the interfacial charge-transfer transition on SSOH. Compared with pure-phase SSOH, the NO abatement ratio of Ag/AgO-SSOH under visible light irradiation is increased to 45.10%. The e in the VB of AgO are excited into the CB under visible light, and are further transferred to the Ag to react with O to produce superoxide radicals. The photo-excited e in the VB of SSOH enter into the VB of AgO through interfacial charge-transfer transition to recombine with the photo-generated holes in the VB of AgO, thereby leaving photo-generated holes in the VB of SSOH. The holes in the VB of SSOH have sufficient oxidizing ability to oxidize the adsorbed hydroxyl groups into hydroxyl radicals. This work provides a new perspective for photocatalytic removal of pollutants by wide band gap photocatalyst under visible light.
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http://dx.doi.org/10.1016/j.envres.2021.111521DOI Listing
June 2021

Zoology, chemical composition, pharmacology, quality control and future perspective of Musk (Moschus): a review.

Chin Med 2021 Jun 19;16(1):46. Epub 2021 Jun 19.

School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, People's Republic of China.

Musk, the dried secretion from the musk sac gland which is located between the navel and genitals of mature male musk deer, is utilized as oriental medicine in east Asia. It has been utilized to treat conditions such as stroke, coma, neurasthenia, convulsions, and heart diseases in China since ancient times. This paper aims to provide a comprehensive overview of musk in zoology, chemical composition, pharmacology, clinical applications, and quality control according to the up-to-date literature. Studies found that musk mainly contains macrocyclic ketones, pyridine, steroids, fatty acids, amino acids, peptides, and proteins, whilst the main active ingredient is muscone. Modern pharmacological studies have proven that musk possesses potent anti-inflammatory effects, neuroprotective effects, anti-cancer effects, antioxidant effects, etc. Moreover, muscone, the main active ingredient, possesses anti-inflammatory, neuroprotective, antioxidant, and other pharmacological effects. In the quality control of musk, muscone is usually the main detection indicator, and the common analytical method is GC, and researchers have established novel and convenient methods such as HPLC-RI, RP-UPLC-ELSD, and Single-Sweep Polarography. In addition, quality evaluation methods based on steroids and the bioactivity of musk have been established. As for the identification of musk, due to various objective factors such as the availability of synthetic Muscone, it is not sufficient to rely on muscone alone as an identification index. To date, some novel technologies have also been introduced into the identification of musk, such as the electronic nose and DNA barcoding technology. In future research, more in vivo experiments and clinical studies are encouraged to fully explain the pharmacological effects and toxicity of musk, and more comprehensive methods are needed to evaluate and control the quality of musk.
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http://dx.doi.org/10.1186/s13020-021-00457-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8214773PMC
June 2021

Removal of trace DNA toxic compounds using a Poly(deep eutectic solvent)@Biomass based on multi-physical interactions.

J Hazard Mater 2021 Jun 9;418:126369. Epub 2021 Jun 9.

College of Pharmaceutical Science, Institute of Life Science and Green Development, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Hebei University, Baoding 071002, China. Electronic address:

DNA toxic compounds (DNA-T-Cs), even in trace amounts, seriously threaten human health and must be completely eliminated. However, the currently used separation media face great challenges in removing trace DNA-T-Cs. Based on the functional advantages of deep eutectic solvents (DESs) and the natural features of biomass (BioM), a series of Poly(DES)@BioMs functioning as adsorbents were prepared for the removal of aromatic/hetero-atomic DNA-T-Cs at the ppm level. After optimisation of experimental conditions, the removal efficiency for DNA-T-Cs ranged from 92.4% to 96.0% with an initial concentration of 20.0 ppm, a temperature of 30 °C, duration of 30 min, and pH of 7.0. The removal processes between the DNA-T-Cs and Poly(DES)@BioMs are well described in the Temkin equilibrium and second-order kinetic adsorption models, and the desorption processes are well shown in the Korsmeryer-Peppas equilibrium and zero-order kinetic models. Molecular simulations revealed that the removal interactions include hydrogen bonding, π-π stacking, and hydrophobic/hydrophilic effects. The removal efficiency for the DNA-T-Cs at 8.0 ppm in industrial sewage ranged from 69.7% to 102%, while the removal efficiency for the DNA-T-Cs standing alone at 20.0 ppm in a methyl violet drug solution was 95.4%, confirming that the Poly(DES)@BioMs effectively removed trace DNA-T-Cs in field samples.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126369DOI Listing
June 2021

Insights Into Host Cell Cytokines in Infection.

Front Immunol 2021 21;12:639834. Epub 2021 May 21.

Institute of Pathogenic Biology, Hengyang Medical College, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, China.

Chlamydial infection causes a number of clinically relevant diseases and induces significant morbidity in humans. Immune and inflammatory responses contribute to both the clearance of infection and pathology in host tissues. infection stimulates host cells to produce a large number of cytokines that trigger and regulate host immune responses against . However, inappropriate responses can occur with excessive production of cytokines, resulting in overreactive inflammatory responses and alterations in host or metabolism. As a result, persists and causes wound healing delays, leading to more severe tissue damage and triggering long-lasting fibrotic sequelae. Here, we summarize the roles of cytokines in infection and pathogenesis, thus advancing our understanding chlamydial infection biology and the pathogenic mechanisms involved.
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http://dx.doi.org/10.3389/fimmu.2021.639834DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176227PMC
May 2021

Photoluminescence with an unusual open-loop and rigid delocalized conjugated structure in quantum dots.

J Colloid Interface Sci 2021 May 23;601:385-396. Epub 2021 May 23.

College of Pharmaceutical Science, Institute of Life Science and Green Development, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Hebei University, Baoding 071002, China. Electronic address:

It is well known that almost all photoluminescent molecules are aromatic or heterocyclic ring compounds for bioimaging analysis. A question remains as to whether a breakthrough can be achieved regarding a novel photoluminescent molecule without a ring structure, and in a what manner. In this study, we explored the photoelectric conversion and structure of photoluminescent compounds, and constructed an intra-molecular coupling positive-negative-junction (PNJ) with an open-loop and rigid Π delocalized conjugated structure of the coupling p-π conjugate system. This was performed to enable strong absorption of the R/tail-end band for the high probability of an n → π*/n → σ* electron transition for photoluminescence production. Subsequently, the Π structure was formed in a short-chain aliphatic molecule as a hydrolytic product of citric acid and urea, and computational methodology was employed to estimate the feasibility of the molecule photoluminescence. Finally, a quantum dot material was fabricated from the aliphatic molecule, the optical properties of the quantum dots were investigated, and the biocompatibility and bioimaging ability of quantum dots were assessed. This work presents not only a theoretical exploration but also practical application of a new strategy to obtain molecules, compounds, and materials with bioimaging.
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http://dx.doi.org/10.1016/j.jcis.2021.05.112DOI Listing
May 2021

Circulating SARS-CoV-2 Vaccine Antigen Detected in the Plasma of mRNA-1273 Vaccine Recipients.

Clin Infect Dis 2021 May 20. Epub 2021 May 20.

Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA.

SARS-CoV-2 proteins were measured in longitudinal plasma samples collected from 13 participants who received two doses of mRNA-1273 vaccine. 11 of 13 participants showed detectable levels of SARS-CoV-2 protein as early as day one after first vaccine injection. Clearance of detectable SARS-CoV-2 protein correlated with production of IgG and IgA.
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http://dx.doi.org/10.1093/cid/ciab465DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241425PMC
May 2021

Imaging and Controlling Photonic Modes in Perovskite Microcavities.

Adv Mater 2021 Jun 13;33(25):e2100775. Epub 2021 May 13.

State Key Laboratory for Artificial Microstructure and Mesoscopic Physics, Department of Physics, Peking University, Beijing, 100871, China.

Perovskite microcavities have excellent photophysical properties for integrated optoelectronic devices, such as nanolasers. Imaging and controlling the photonic modes within the cavity are fundamentally important to understand and develop applications. Here, photoemission electron microscopy (PEEM) is used to image the photonic modes within optical microcavities with a nanometer-scale spatial resolution. From a CsPbBr microcavity, hybrid mode patterns are observed. Spatial frequency spectrum analysis on the patterns uncovers the characteristic cavity modes, which are modeled with transverse magnetic (TM) and transverse electric (TE) waves, and assigned to exciton-polariton modes. Based on this understanding, the light focus in a designed microcavity is imaged in real space and controlled by the light field polarization. The study confirms that the cavity modes in perovskites can be effectively observed by the PEEM technique under resonant excitation, which, in turn, promotes the design of optoelectronic devices based on perovskite microcavities.
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http://dx.doi.org/10.1002/adma.202100775DOI Listing
June 2021

T cell membrane cloaking tumor microenvironment-responsive nanoparticles with a smart "membrane escape mechanism" for enhanced immune-chemotherapy of melanoma.

Biomater Sci 2021 May;9(9):3453-3464

College Pharmacy, Jiamusi University, 258 Xuefu Street, Jiamusi, Heilongjiang 154007, China.

The application of combination immune-chemotherapy makes up for the limitation of monotherapy and achieves superior antitumor activity against cancer. However, combinational therapy is always restricted by poor tumor targeted drug delivery efficacy. Herein, novel T cell membrane cloaking tumor microenvironment-responsive nanoparticles (PBA modified T cell membrane cloaking hyaluronic acid (HA)-disulfide bond-vitamin E succinate/curcumin, shortened as [email protected]) were developed. T cell membrane cloaking not only serves as a protection shell for sufficient drug delivery but also acts as a programmed cell death-1(PD-1) "antibody" to selectively bind the PD-L1 of tumor cells. When [email protected] is intravenously administrated into the blood stream, it accumulates at tumor sites and responds to an acidic pH to achieve a "membrane escape effect" and expose the HA residues of RCM for tumor targeted drug delivery. RCM accumulates in the cytoplasm via CD44 receptor mediated endocytosis and intracellularly releases antitumor drug in the intracellular redox microenvironment for tumor chemotherapy. T cell membrane debris targets the PD-L1of tumor cells for tumor immunotherapy, which not only directly kills tumor cells, but also improves the CD8+ T cell level and facilitates effector cytokine release. Taken together, the as-constructed [email protected] creates a new way for the rational design of a drug delivery system via the combination of stimuli-responsive drug release, chemotherapeutical agent delivery and cell membrane based immune checkpoint blockade immunotherapy.
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http://dx.doi.org/10.1039/d1bm00331cDOI Listing
May 2021

Tunable graphdiyne for DNA surface adsorption: affinities, displacement, and applications for fluorescence sensing.

Anal Bioanal Chem 2021 Jun 2;413(15):3847-3859. Epub 2021 May 2.

Key Laboratory of Theoretical Organic Chemistry and Function Molecule, Ministry of Education Hunan University of Science and Technology, Xiangtan, 411201, Hunan, China.

Graphdiyne (GDY) adsorbed DNA probes have been used as a fluorescent sensing platform, but topics including DNA adsorption affinities, DNA probe displacement, and fluorescence quenching ability were rarely researched. Herein, the adsorption affinity of single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) on a tremella-like GDY was tuned by modulating the surface chemistry of GDY. The fluorescence quenching ability of GDY with different oxidation degrees was compared. The nonspecific displacement of DNA probes on GDY was studied. Under the same concentrations, GDY with low oxidation degree exhibited stronger adsorption affinity and higher adsorption capacity to both ssDNA and dsDNA than highly oxidized GDY. DNA adsorbed on low-oxidized GDY was more resistant to displacement by other DNAs. Protein showed strong interaction with different GDY and could displace DNA probes on GDY. Based on these findings, an ideal GDY with proper oxidation degree, exhibiting high surface affinity for ssDNA and low affinity for dsDNA, was used as scavenger of redundant ssDNA fluorescent probe in an enzyme-assisted amplification system for sensitive ochratoxin (OTA) detection. This study has enhanced our fundamental understanding of DNA adsorption by GDY. It also provided a rational way to apply GDY for fluorescence sensing in a complicated system.
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http://dx.doi.org/10.1007/s00216-021-03355-yDOI Listing
June 2021

Metagenome-assembled genomes infer potential microbial metabolism in alkaline sulphidic tailings.

Environ Microbiome 2021 Apr 29;16(1). Epub 2021 Apr 29.

Hebei Key Laboratory of Soil Ecology, Key Laboratory for Agricultural Water Resource, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050021, China.

Background: Mine tailings are hostile environment. It has been well documented that several microbes can inhabit such environment, and metagenomic reconstruction has successfully pinpointed their activities and community structure in acidic tailings environments. We still know little about the microbial metabolic capacities of alkaline sulphidic environment where microbial processes are critically important for the revegetation. Microbial communities therein may not only provide soil functions, but also ameliorate the environment stresses for plants' survival.

Results: In this study, we detected a considerable amount of viable bacterial and archaeal cells using fluorescent in situ hybridization in alkaline sulphidic tailings from Mt Isa, Queensland. By taking advantage of high-throughput sequencing and up-to-date metagenomic binning technology, we reconstructed the microbial community structure and potential coupled iron and nitrogen metabolism pathways in the tailings. Assembly of 10 metagenome-assembled genomes (MAGs), with 5 nearly complete, was achieved. From this, detailed insights into the community metabolic capabilities was derived. Dominant microbial species were seen to possess powerful resistance systems for osmotic, metal and oxidative stresses. Additionally, these community members had metabolic capabilities for sulphide oxidation, for causing increased salinity and metal release, and for leading to N depletion.

Conclusions: Here our results show that a considerable amount of microbial cells inhabit the mine tailings, who possess a variety of genes for stress response. Metabolic reconstruction infers that the microbial consortia may actively accelerate the sulphide weathering and N depletion therein.
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http://dx.doi.org/10.1186/s40793-021-00380-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086273PMC
April 2021

Enhanced Thermoelectric Performance in High Entropy Alloys SnPbMnGeTe.

ACS Appl Mater Interfaces 2021 Apr 13;13(16):18638-18647. Epub 2021 Apr 13.

School of Materials Science and Engineering, and Institute of Materials Genome and Big Data, Harbin Institute of Technology, Shenzhen 518055, P.R. China.

Entropy is a physical quantity gauging the degree of chaos in the system. High entropy alloying is thus an effective strategy to reduce the lattice thermal conductivity of the thermoelectric materials. In this paper, PbTe, GeTe, and MnTe are coalloyed with SnTe to form a single-phase solid solution. Because of the inclusion of various elements at the cationic (Sn) site, the configurational entropy increases, and the phonon scattering is strongly enhanced, leading to a reduced lattice thermal conductivity. In addition, the Seebeck coefficient is improved because of the band modification via this coalloying. Ga is then further doped to optimize the carrier concentration to ∼5.7 × 10 cm and reduce the room-temperature lattice thermal conductivity to ∼0.6 W m K. Finally, a high peak ZT value of ∼1.52 at 823 K and an average ZT value ∼1.0 from 323 to 823 K were obtained in Ga(SnPbMnGe)Te.
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http://dx.doi.org/10.1021/acsami.1c00221DOI Listing
April 2021

Metagenomic next-generation sequencing for identifying pathogens in central nervous system complications after allogeneic hematopoietic stem cell transplantation.

Bone Marrow Transplant 2021 Apr 6. Epub 2021 Apr 6.

Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

A prospective study was conducted to compare metagenomic next-generation sequencing (mNGS) and conventional testing in investigating the pathogens of central nervous system (CNS) infections in allogeneic hematopoietic stem cell transplantation (allo-HSCT) recipients. A total of 53 patients with CNS disorders after allo-HSCT were enrolled in this study. A total of 35 patients were diagnosed as CNS infections, including 28 viral, 2 bacterial, 1 fungal, 3 mixed infections, and 1 infection with unknown pathogen. Among these 35 patients with CNS infections, mNGS identified 5 patients who were not identified by conventional testing. For the remaining 30 infections, mNGS made concurrent diagnoses with conventional testing in 29, while 1 was diagnosed according to the good response to the antimicrobial treatment without etiological evidence. The presence of Aspergillus detected by mNGS only in one patient was considered false positive due to lack of validation. The sensitivity of mNGS and conventional testing for diagnosing CNS infections post transplant were 97.1% and 82.9%, respectively (P = 0.106), while the specificity of mNGS and conventional testing were 94.4% and 100%, respectively (P = 1.000). These results suggest that mNGS might be a promising technology for diagnosis of CNS infections post transplant. Viruses were the most common pathogens of CNS infections in allo-HSCT recipients.
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http://dx.doi.org/10.1038/s41409-021-01243-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8023769PMC
April 2021

Effect of Different Quaternary Ammonium Groups on the Hydroxide Conductivity and Stability of Anion Exchange Membranes.

ACS Omega 2021 Mar 15;6(12):7994-8001. Epub 2021 Mar 15.

Chemical Engineering Department, College of Engineering, University of Ha'il, P.O. Box 2440, Ha'il 81441, Saudi Arabia.

Anion exchange membrane fuel cells (AEMFCs) are encouraging electrochemical structures for the competent and complaisant conversion of energy. Herein, the development of brominated poly(2,6-dimethyl phenylene oxide) (BPPO)-based anion exchange membranes (AEMs) with different quaternary ammonium groups for AEMFCs was reported. The successful preparation of AEMs was proved by utilizing proton nuclear magnetic resonance and Fourier transform infrared spectroscopy. They were explored in terms of water uptake ( ), ion exchange capacity (IEC), hydration number (λ), linear swelling ratio (LSR), morphology, tensile strength (TS), and elongation at break ( ). The alkaline stability of the prepared AEMs was assessed and compared with each other. The experimental outcomes demonstrated that the -methylpyrrolidinium-based membrane (MPyPPO) exhibited higher alkaline stability, whereas the -methylimidazolium-based membrane (MImPPO) showed the lowest alkaline stability among the prepared AEMs. Similarly, the hydroxide conductivity of the prepared AEMs was measured and compared with each other. The pyrrolidinium-based membrane (MPyPPO) exhibited higher hydroxide conductivity among the prepared AEMs.
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http://dx.doi.org/10.1021/acsomega.0c05134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8014933PMC
March 2021

Farmland heavy metals can migrate to deep soil at a regional scale: A case study on a wastewater-irrigated area in China.

Environ Pollut 2021 Jul 22;281:116977. Epub 2021 Mar 22.

School of Environment, Nanjing University, Nanjing, 210093, China. Electronic address:

Heavy metal risks to human health in farmland of wastewater-irrigated areas have long been recognized. It remains to be shown whether farmland heavy metals from wastewater irrigation can migrate to deeper soil at a regional scale. In this study, nine soil cores deep to 30 m from three transects (A, B and C) of a linear wastewater reservoir and the adjacent farmland topsoils and wheat grains were sampled. Heavy metals including As, Cd, Cr, Cu, Pb and Zn in the soils and wheat grains were determined, and the grains' health risks were assessed using the Target Hazard Quotient (THQ). Considerably high contents of heavy metals in both total and soluble forms were detected in deep soils, especially for the transect B where total As of 73.0 mg kg at 29 m, Cd of 3.80 mg kg at 13 m and Pb of 214 mg kg at 30 m were detected. The silty clayey and silty layers of the transect B had higher contents of As, Cr, Cu, Pb and Zn compared with the sandy layers. Across the studied area, 19.5%-34.1% of the topsoil samples were contaminated by As, Cd, Cu, Pb and Zn, and 34.1% and 19.5% of the wheat grains were contaminated by Cd and Pb, respectively. Wheat grains from all the sampling sites had a combined target hazard quotient (TTHQ) value of >1, with As and Cd being the most important contributors. Our study revealed a wider and deeper risk of typical heavy metals originated from long-term wastewater irrigation in the sampling area, which may pose substantial health risks to the local residents via wheat grains and groundwater.
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http://dx.doi.org/10.1016/j.envpol.2021.116977DOI Listing
July 2021

Mapping Trap Dynamics in a CsPbBr Single-Crystal Microplate by Ultrafast Photoemission Electron Microscopy.

Nano Lett 2021 Apr 24;21(7):2932-2938. Epub 2021 Mar 24.

State Key Laboratory for Artificial Microstructure and Mesoscopic Physics, Department of Physics, Peking University, Beijing 100871, China.

For versatile lead-halide perovskite materials, their trap states, both in the bulk and at the surface, significantly influence optoelectronic behaviors and the performance of the materials and devices. Direct observation of the trap dynamics at the nanoscale is necessary to understand and improve the device design. In this report, we combined the femtosecond pump-probe technique and photoemission electron microscopy (PEEM) to investigate the trap states of an inorganic perovskite CsPbBr single-crystal microplate with spatial-temporal-energetic resolving capabilities. Several shallow trap sites were identified within the microplate, while the deep traps were resolved throughout the surface. The results revealed high-defect tolerance to the shallow traps, while the surface dynamics were dominated by the surface deep traps. The ultrafast PEEM disclosed a full landscape of fast electron transfer and accumulation of the surface trap states. These discoveries proved the excellent electronic properties of perovskite materials and the importance of surface optimization.
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http://dx.doi.org/10.1021/acs.nanolett.1c00014DOI Listing
April 2021

Unprecedented Application of Covalent Organic Frameworks for Polymerization Catalysis: Rh/TPB-DMTP-COF in Polymerization of Phenylacetylene and Its Functional Derivatives.

ACS Appl Mater Interfaces 2021 Mar 12;13(11):13693-13704. Epub 2021 Mar 12.

Key Laboratory of Cluster Science of Ministry of Education, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, China.

Covalent organic frameworks (COFs) are applied widely in organic catalysis; however, no precedent has been reported in polymerization catalysis. Herein, we report the new application of COFs for polymerization catalysis. Different amounts of homogeneous Rh catalyst are incorporated into the COF via post-treatment to give a series of TPB-DMTP-COF-X wt % Rh (-) containing varying amounts of Rh from 2.74 to 11.38 wt %. In contrast to the known Rh catalysts, TPB-DMTP-COF-X wt % Rh (-) display an uncommon synergistic effect and exceptional steric confinement effect of nanochannels. Therefore, they possess the advantages of both homogeneous catalysts in high activity and selectivity and heterogeneous catalysts in stability and recyclability with extremely high activity up to 1.3 × 10 g·mol·h and -selectivity up to 99% and can be readily recycled and reused five times in the polymerization of phenylacetylene and its derivatives, affording polyphenylacetylene and its derivatives having helical structures, aggregation-induced emission properties, or fluorescence properties with narrow molecular weight distributions.
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http://dx.doi.org/10.1021/acsami.1c00512DOI Listing
March 2021

Salidroside: A review of its recent advances in synthetic pathways and pharmacological properties.

Chem Biol Interact 2021 Apr 20;339:109268. Epub 2021 Feb 20.

School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China. Electronic address:

Salidroside has been identified as one of the most potent compounds isolated from various Rhodiola plants, which have been used for a long time as adaptogens in traditional Chinese medicine. However, due to the severe growing environment of herbal medicine and large-scale excavation, the content of natural salidroside is extremely small. Most of the previous studies focused on herbal medicine, and there were few reviews on the synthesis of its main active ingredient salidroside. This paper presents different synthetic routes of salidroside to resolve the contradiction between supply and demand and lays the foundation for new drug research and development. Furthermore, emerging evidence indicates that salidroside, a promising environmentally-adapted drug with low toxicity and few side effects, possesses a wide spectrum of pharmacological properties, including activities on the cardiovascular system and central nervous system, anti-hypoxia, anti-fatigue and anti-aging activities, anticancer activity, anti-inflammatory activity, antioxidant activity, antivirus and immune stimulation activities, antidiabetic activity, anti-osteoporotic activity, and so on. Although the former researches have summarized the pharmacological effects of salidroside, focusing on the central nervous system, diabetes, and cancer, the overall pharmacological aspects of it have not been analyzed. This review highlights biological characteristics and mechanisms of action from 2009 to now as well as toxicological and pharmacokinetic data of the analyzed compound reported so far, with a view to providing a reference for further development and utilization of salidroside.
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http://dx.doi.org/10.1016/j.cbi.2020.109268DOI Listing
April 2021

Corrigendum: The Application of Regulatory Cascades in : Yield Enhancement and Metabolite Mining.

Front Microbiol 2020 3;11:614274. Epub 2021 Feb 3.

Institute of Biopharmaceuticals, Taizhou University, Taizhou, China.

[This corrects the article DOI: 10.3389/fmicb.2020.00406.].
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http://dx.doi.org/10.3389/fmicb.2020.614274DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888258PMC
February 2021

Ordered Macroporous Superstructure of Nitrogen-Doped Nanoporous Carbon Implanted with Ultrafine Ru Nanoclusters for Efficient pH-Universal Hydrogen Evolution Reaction.

Adv Mater 2021 Mar 18;33(12):e2006965. Epub 2021 Feb 18.

AIST-Kyoto University Chemical Energy Materials Open Innovation Laboratory (ChEM-OIL), National Institute of Advanced Industrial Science and Technology (AIST), Sakyo-ku, Kyoto, 6068501, Japan.

The electrochemical hydrogen evolution reaction (HER) is an attractive technology for the mass production of hydrogen. Ru-based materials are promising electrocatalysts owing to the similar bonding strength with hydrogen but much lower cost than Pt catalysts. Herein, an ordered macroporous superstructure of N-doped nanoporous carbon anchored with the ultrafine Ru nanoclusters as electrocatalytic micro/nanoreactors is developed via the thermal pyrolysis of ordered macroporous single crystals of ZIF-8 accommodating Ru(III) ions. Benefiting from the highly interconnected reticular macro-nanospaces, this superstrucure affords unparalleled performance for pH-universal HER, with order of magnitude higher mass activity compared to the benchmark Pt/C. Notably, an exceptionally low overpotential of only 13 [email protected] mA cm is required for HER in alkaline solution, with a low Tafel slope of 40.41 mV dec and an ultrahigh turnover frequency value of 1.6 H s at 25 mV, greatly outperforming Pt/C. Furthermore, the hydrogen generation rates are almost twice those of Pt/C during practical overall alkaline water splitting. A solar-to-hydrogen system is also demonstrated to further promote the application. This research may open a new avenue for the development of advanced electrocatalytic micro/nanoreactors with controlled morphology and excellent performance for future energy applications.
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http://dx.doi.org/10.1002/adma.202006965DOI Listing
March 2021

Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylation in Candida albicans.

mSystems 2021 Feb 2;6(1). Epub 2021 Feb 2.

Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing, Jiangsu, People's Republic of China

is the most common human fungal pathogen, causing diseases ranging from mucosal to systemic infections for both immunocompetent and immunocompromised individuals. Lysine 2-hydroxyisobutyrylation is a highly conserved posttranslational modification found in a wide variety of organisms. In this study, we surveyed the biological impact of 2-hydroxyisobutyrylation on lysine residuals (Khib) in Using an antibody enrichment approach along with the traditional liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, we analyzed the pattern of Khib-modified proteins and sites in one wild-type strain of We identified 1,438 Khib-modified proteins with 6,659 modified sites in this strain, and a more detailed bioinformatics analysis indicated that the Khib-modified proteins are involved in a wide range of cellular functions with diverse subcellular locations. Functional enrichment analysis featured several prominent functional pathways, including ribosome, biosynthesis of antibiotics, biosynthesis of secondary metabolites, biosynthesis of amino acids and carbon metabolism, of which the ribosome pathway is the most affected pathway. Even compared with the reported numbers of lysine acetylation (Kac) and succinylation (Ksuc) sites, the numbers of Khib-modified sites on ribosomal proteins remained the highest for These bioinformatic results suggest that 2-hydroxyisobutyrylation may play an indispensable role in the regulation of the ribosomal biogenesis and protein translation. Findings in this study may provide new insights for studying posttranslational modification (PTM)-associated mechanisms in fungal development and pathogenicity. is one of the most commonly reported fungal pathogens in mucosal and systemic infections. A better understanding of its growth habits and metabolic processes in the host should help improve defense strategies. The newly discovered protein posttranslational modification (PTM) on histones is one epigenetic mechanism which has been linked to many pathogenic events, including cancers. The types of PTM and their pathogenic roles in are still somewhat poorly understood, even though studies of based on acetylation inhibitors have shed some light on their function, and it seems that PTMs regulate pathogenic adhesion factors. Here, we quantified and analyzed the occurrence of lysine 2-hydroxyisobutyrylation (Khib) in The Khib-modified proteins are enriched with respect to carbon metabolism, ribosomal biogenesis, and protein translation in .
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http://dx.doi.org/10.1128/mSystems.01129-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7857534PMC
February 2021

A consensus model to manage the non-cooperative behaviors of individuals in uncertain group decision making problems during the COVID-19 outbreak.

Appl Soft Comput 2021 Feb 9;99:106879. Epub 2020 Nov 9.

Business School, Sichuan University, Chengdu 610064, China.

The COVID-19 pandemic has brought lots of losses to the global economy. Within the context of COVID-19 outbreak, many emergency decision-making problems with uncertain information arose and a number of individuals were involved to solve such complicated problems. For instance, the selection of the first entry point to China is important for oversea flights during the epidemic outbreak given that reducing imported virus from abroad becomes the top priority of China since China has achieved remarkable achievements regarding the epidemic control. In such a large-scale group decision making problem, the non-cooperative behaviors of experts are common due to the different backgrounds of the experts. The non-cooperative behaviors of experts have a negative impact on the efficiency of a decision-making process in terms of decision time and cost. Given that the non-cooperative behaviors of experts were rarely considered in existing large-scale group decision making methods, this study aims to propose a novel consensus model to manage the non-cooperative behaviors of experts in large-scale group decision making problems. A group consistency index simultaneously considering fuzzy preference values and cooperation degrees is introduced to detect the non-cooperative behaviors of experts. We combine the cooperation degrees and fuzzy preference similarities of experts when clustering experts. To reduce the negative influence of the experts with low degrees of cooperation on the quality of a decision-making process, we implement a dynamic weight punishment mechanism to non-cooperative experts so as to improve the consensus level of a group. An illustrative example about the selection of the first point of entry for the flights entering Beijing from Toronto during the COVID-19 outbreak is presented to show the validity of the proposed model.
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http://dx.doi.org/10.1016/j.asoc.2020.106879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7831966PMC
February 2021

Systematic Analysis of the Lysine Crotonylome and Multiple Posttranslational Modification Analysis (Acetylation, Succinylation, and Crotonylation) in Candida albicans.

mSystems 2021 Jan 26;6(1). Epub 2021 Jan 26.

Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing, China

is an opportunistic pathogen that causes lethal fungal infections in immunocompromised patients. Lysine crotonylation is a newly discovered PTM (posttranslational modification) epigenetic type that may play a critical role in regulating gene expression. In this study, we used an antibody-enrichment approach along with LC-MS/MS to carry out a quantitative crotonylome analysis in We found a total of 5,242 crotonylation sites and 1,584 crotonylated proteins among 9,038 proteins in this organism. Of these crotonylated proteins, a few unique crotonylated motifs are noted such as D and E in positions +1, +2, or +3 or K and R in positions +5 or +6, while A, E, F, G, P, W, and Y are in the -1 position or A, K, and R are found in positions -5, -6, -7, or -8. Functional analysis has shown that a majority of the crotonylated proteins are related to biosynthetic events and carbon metabolism. When combined with previously collected data on acetylation and succinylation, PPI (protein-protein interaction network) analysis reveals that proteins with functions in ribosomal biogenesis, oxidative phosphorylation, nucleus activity, and proteasome formation are heavily modified by these three PTM types. To the best of our knowledge, this is the first crotonylome study carried out in and is an important step to a better understanding of the biological and pathogenic impact of PTM in is a kind of pathogen of fungal infections that is found worldwide. Lysine crotonylation of proteins as a recently discovered PTM (posttranslational modification) may have a critical role in regulating cells. We first carried out large-scale analysis of crotonylated proteome and multiple PTM analysis (acetylation, succinylation, and crotonylation), then drew a diagram to show multiple PTM sites on histones in of our study. This study about crotonylome in human pathogenic fungi is a milestone that first and deeply investigates the functional analysis of crotonylated proteins in , which marks an important start for further research.
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http://dx.doi.org/10.1128/mSystems.01316-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7842366PMC
January 2021

Spectromicroscopy and imaging of photoexcited electron dynamics at in-plane silicon pn junctions.

Nanoscale 2021 Feb;13(4):2626-2631

State Key Laboratory for Mesoscopic Physics, Collaborative Innovation Center of Quantum Matter, Nano-optoelectronics Frontier Center of the Ministry of Education, School of Physics, Peking University, Beijing 100871, China. and Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan, Shanxi 030006, China and Peking University Yangtze Delta Institute of Optoelectronics, Nantong 226010, Jiangsu, China.

The ultrafast spatiotemporal imaging of photoexcited electrons is essential to understanding interfacial electron dynamic processes. We used time- and energy-resolved photoemission electron microscopy (PEEM) to investigate the photoexcited electron dynamics at multiplex in-plane silicon pn junctions. We found that the measured kinetic energy of photoelectrons from n-type regions is higher than that from p-type regions owing to different work functions. Interestingly, the kinetic energy of outer n-type regions is higher than that of inner n-type regions, which is caused by the reverse bias induced by photoemission. Time-resolved PEEM results reveal different evolution rates of hot electrons in different doping regions. The rise time of the n-type (outer n-type) regions is faster than that of the p-type (inner n-type) regions. So, closed doping patterns can influence the electron spectra and dynamics at the micro-nano scale. These results help us to understand the ultrafast dynamics of carriers at in-plane interfaces and optimize optoelectronic integrated devices with complex heterojunctions.
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http://dx.doi.org/10.1039/d0nr07954eDOI Listing
February 2021

From Antiaromatic Norcorrolatonickel(II) to Aromatic and Nonaromatic Zwitterions: Innocent Ligands with Unbalanced Charge of the Core.

Org Lett 2021 Feb 21;23(3):1032-1037. Epub 2021 Jan 21.

Department of Chemistry, University of Wrocław, F. Joliot-Curie 14, 50 383 Wrocław, Poland.

A three-component reaction of antiaromatic -mesityl-3-nitronorcorrolatonickel(II) -NO with dialkyl acetylenedicarboxylate and PBu yields aromatic zwitterionic corrole nickel(II) complexes -R with one of the -substituents comprising a positively charged tributyl phosphonium group and negatively charged coordination core. Reaction of -NO with PBu alone resulted in a nonaromatic chiral adduct of a zwitterionic phlorine character with a -PBu group at a pyrrole β-position.
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http://dx.doi.org/10.1021/acs.orglett.0c04227DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7875510PMC
February 2021

Environmental noise stress disturbs commensal microbiota homeostasis and induces oxi-inflammmation and AD-like neuropathology through epithelial barrier disruption in the EOAD mouse model.

J Neuroinflammation 2021 Jan 6;18(1). Epub 2021 Jan 6.

Institute of Environmental and Operational Medicine, Chinese Academy of Military Medical sciences, Tianjin, China.

Background: Both genetic factors and environmental hazards, including environmental noise stress, have been associated with gut microbiome that exacerbates Alzheimer's disease (AD) pathology. However, the role and mechanism of environmental risk factors in early-onset AD (EOAD) pathogenesis remain unclear.

Methods: The molecular pathways underlying EOAD pathophysiology following environmental noise exposure were evaluated using C57BL/6 wild-type (WT) and APP/PS1 Tg mouse models. The composition differences in intestinal microbiota were analyzed by 16S rRNA sequencing and Tax4Fun to predict the metagenome content from sequencing results. An assessment of the flora dysbiosis-triggered dyshomeostasis of oxi-inflamm-barrier and the effects of the CNS end of the gut-brain axis was conducted to explore the underlying pathological mechanisms.

Results: Both WT and APP/PS1 mice showed a statistically significant relationship between environmental noise and the taxonomic composition of the corresponding gut microbiome. Bacterial-encoded functional categories in noise-exposed WT and APP/PS1 mice included phospholipid and galactose metabolism, oxidative stress, and cell senescence. These alterations corresponded with imbalanced intestinal oxidation and anti-oxidation systems and low-grade systemic inflammation following noise exposure. Mechanistically, axis-series experiments demonstrated that following noise exposure, intestinal and hippocampal tight junction protein levels reduced, whereas serum levels of inflammatory mediator were elevated. Regarding APP/PS1 overexpression, noise-induced abnormalities in the gut-brain axis may contribute to aggravation of neuropathology in the presymptomatic stage of EOAD mice model.

Conclusion: Our results demonstrate that noise exposure has deleterious effects on the homeostasis of oxi-inflamm-barrier in the microbiome-gut-brain axis. Therefore, at least in a genetic context, chronic noise may aggravate the progression of EOAD.
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http://dx.doi.org/10.1186/s12974-020-02053-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789697PMC
January 2021

Calycosin: a Review of its Pharmacological Effects and Application Prospects.

Expert Rev Anti Infect Ther 2021 Jul 21;19(7):911-925. Epub 2020 Dec 21.

School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, PR China.

: Calycosin (CA), a typical phytoestrogen extracted from root of . On the basis of summarizing the pharmacological and pharmacokinetic studies of CA in recent years, we hope to provide useful information for CA about treating different diseases and to make suggestions for future research.: We collected relevant information (January 2014 to March 2020) on CA via the Internet database. Keywords searched includ pharmacology, pharmacokinetics and toxicology, and the number of effective references was 118. CA is a phytoestrogen with wide range of pharmacological activities. By affecting PI3K/Akt/mTOR, WDR7-7-GPR30, Rab27B-β-catenin-VEGF, etc. signaling pathway, CA showed the effect of anticancer, anti-inflammatory, anti-osteoporosis, neuroprotection, hepatoprotection, etc. Therefore, CA is prospective to be used in the treatment of many diseases.: Research shows that CA has a therapeutic effect on a variety of diseases. We think CA is a promising natural medicine. Therefore, we propose that the research directions of CA in the future include the following. Carrying out clinical research trials in order to find the most suitable medicinal concentration for different diseases; Exploring the synergistic mechanism of CA in combination with other drugs; Exploring ways to increase the blood circulation concentration of CA.
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http://dx.doi.org/10.1080/14787210.2021.1863145DOI Listing
July 2021

Lupeol and its derivatives as anticancer and anti-inflammatory agents: Molecular mechanisms and therapeutic efficacy.

Pharmacol Res 2021 02 11;164:105373. Epub 2020 Dec 11.

School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, PR China. Electronic address:

Lupeol is a natural triterpenoid that widely exists in edible fruits and vegetables, and medicinal plants. In the last decade, a plethora of studies on the pharmacological activities of lupeol have been conducted and have demonstrated that lupeol possesses an extensive range of pharmacological activities such as anticancer, antioxidant, anti-inflammatory, and antimicrobial activities. Pharmacokinetic studies have indicated that absorption of lupeol by animals was rapid despite its nonpolar characteristics, and lupeol belongs to class II BCS (biopharmaceutics classification system) compounds. Moreover, the bioactivities of some isolated or synthesized lupeol derivatives have been investigated, and these results showed that, with modification to C-3 or C-19, some derivatives exhibit stronger activities, e.g., antiprotozoal or anticancer activity. This review aims to summarize the advances in pharmacological and pharmacokinetic studies of lupeol in the last decade with an emphasis on its anticancer and anti-inflammatory activities, as well as the research progress of lupeol derivatives thus far, to provide researchers with the latest information, point out the limitations of relevant research at the current stage and the aspects that should be strengthened in future research.
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http://dx.doi.org/10.1016/j.phrs.2020.105373DOI Listing
February 2021
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