Publications by authors named "Xi Wang"

2,270 Publications

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Hepatocellular adenoma: Where are we now?

World J Gastroenterol 2022 Apr;28(14):1384-1393

Department of Pathology, Yale University School of Medicine, New Haven, CT 06520, United States.

Hepatocellular adenoma (HCA) is a benign hepatocellular neoplasm, commonly occurs in young women with a history of oral contraceptive use. Complications including hemorrhage and malignant transformation necessitate the need for a thorough understanding of the underlying molecular signatures in this entity. Recent molecular studies have significantly expanded our knowledge of HCAs. The well-developed phenotype-genotype classification system improves clinical management through identifying "high risk" subtype of HCAs. In this article, we attempt to provide updated information on clinical, pathologic and molecular features of each subtype of HCAs.
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http://dx.doi.org/10.3748/wjg.v28.i14.1384DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048476PMC
April 2022

Incremental Value of Noncontrast Chest Computed Tomography-derived Parameters in Predicting Subclinical Carotid Atherosclerosis: From the PERSUADE Study.

J Thorac Imaging 2022 May 4. Epub 2022 May 4.

Department of Cardiology, the Sixth Medical Centre.

Purpose: To investigate the incremental value of noncontrast chest computed tomography (CT)-derived parameters, such as coronary artery calcium score (CACS) and epicardial adipose tissue volume (EATv), in predicting subclinical carotid atherosclerosis above traditional risk factors in community-based asymptomatic populations of northern China.

Materials And Methods: A total of 2195 community-based asymptomatic individuals were enrolled from Jidong Oilfield in accordance with the PERSUADE study. CACS and EATv were measured on noncontrast chest CT. Demographics and ideal cardiovascular health score (ICHS) were collected through questionnaires. We recalculated the ideal cardiovascular health risk score (ICHRS) (ICHRS=14-ICHS) and standardized the parameters as log-CACS and body mass index adjusted EATv (i-EATv). Subclinical carotid atherosclerosis was assessed by Doppler sonography and defined as any prevalence of average carotid intima-media thickness ≥1.00 mm, appearance of carotid plaque, and carotid arterial stenosis in the areas of extracranial carotid arteries on both sides.

Results: A total of 451 (20.55%) individuals presented subclinical carotid atherosclerosis. CACS and EATv were significantly greater in the subclinical group, while ICHS was lower. In multivariate logistic regression, ICHRS (odds ratio [OR]=1.143, 95% confidence interval [CI]: 1.080-1.210, P<0.001), log-CACS (OR=1.701, 95% CI: 1.480-1.955, P<0.001), and i-EATv (OR=1.254, 95% CI: 1.173-1.341, P<0.001) were found to be independent risk predictors for subclinical carotid atherosclerosis. In receiver-operating characteristic curve analysis, when combined with male sex and age level, the area under the curve of the ICHRS basic model increased from 0.627 (95% CI: 0.599-0.654) to 0.757 (95% CI: 0.732-0.781) (P<0.0001). Further adding log-CACS and i-EATv, the area under the curve demonstrated a statistically significant improvement (0.788 [95% CI: 0.765-0.812] vs. 0.757 [95% CI: 0.732-0.781], P<0.0001).

Conclusion: Noncontrast chest CT-derived parameters, including CACS and EATv, could provide significant incremental improvement for predicting subclinical carotid atherosclerosis beyond the conventional risk assessment model based on ICHRS.
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http://dx.doi.org/10.1097/RTI.0000000000000655DOI Listing
May 2022

The functional role of fecal microbiota transplantation on Salmonella Enteritidis infection in chicks.

Vet Microbiol 2022 May 5;269:109449. Epub 2022 May 5.

Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, PR China; School of Life Sciences, Shandong Normal University, Jinan 250014, PR China; Key Laboratory of Livestock and Poultry Multi-omics of MARA, Jinan 250100, PR China. Electronic address:

The intestinal microbiota plays important roles in animal health and growth. We investigated the efficacy and mechanisms of fecal microbiota transplantation (FMT) from adult SPF chickens against Salmonella Enteritidis (SE) infection in chicks. We transplanted 160 recipient SPF chicks (1-day-old) that were randomly divided into four groups, Ca (challenge), Cb (non-challenge), Fa (FMT and challenge) and Fb (FMT without challenge). The experiment lasted 40 days. We found that FMT reduced mortality as well as liver inflammatory lesions, promoted weight gain, improved immunity, ameliorated the digestion and absorption ability and inhibited SE colonization in the liver of challenged chicks. 16S rRNA gene high-throughput sequencing indicated that SE challenge caused a significant increase in the relative abundance of Parasutterella in the cecal microbiota of the recipient chicks (P < 0.05). FMT led to the maturation of the intestinal flora of recipients and the relative abundance of the Bacteroides, Rikenellaceae_ RC9_ gut_ group, Prevotellaceae_ UCG_ 001, Prevotellaceae_ Ga6A1_ group and Parabacteroides was significantly increased (P < 0.05). FMT from adult SPF chickens regulated the intestinal microbiota of chicks and increased resistance to SE infection.
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http://dx.doi.org/10.1016/j.vetmic.2022.109449DOI Listing
May 2022

Investigation of selenium nutritional status and dietary pattern among children in Kashin-Beck disease endemic areas in Shaanxi Province, China using duplicate portion sampling method.

Environ Int 2022 Apr 22;164:107255. Epub 2022 Apr 22.

School of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, Collaborative Innovation Center of Endemic Disease and Health Promotion for Silk Road Region, 710061, PR China; Department of Occupational and Environmental Health, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, PR China. Electronic address:

Background And Objectives: Selenium deficiency is a primary risk factor of Kashin-Beck disease (KBD). This study aimed to investigate whether children in endemic areas could maintain sufficient selenium intake after termination of selenium supplement administration, and evaluate their comprehensive nutritional status and dietary structure.

Methods: Duplicate portion sampling combined with a questionnaire was adopted to collect data on categories and quantity of all food ingested in three consecutive days. Occipital hair was also collected to detect selenium content by hydride generation atomic fluorescence spectrometry (HGAFS). CDGSS3.0 software and factor analysis were integrated to assess the children's comprehensive nutritional status and dietary structure.

Results: This study included 240 sex-matched (1:1) children aged 7-12 years from KBD endemic (n = 120) and non-endemic (n = 120) areas. Overall, 720 solid food, 720 liquid, and 240 hair samples were collected for selenium determination. The mean selenium level in hair of children in endemic areas (0.38 ± 0.16 mg/kg) was significantly lower than that in children in non-endemic areas (0.56 ± 0.28 mg/kg, Z = -5.249, p < 0.001). The dietary selenium intake of children in endemic areas was 40.0% lower than that in children in non-endemic areas (Z = -9.374, p < 0.001). Children in endemic areas consumed significantly less diverse dietary items leading to significantly less intake of multiple nutrients compared to children in non-endemic areas.

Conclusions: The dietary selenium intake of most children in endemic areas was less than the recommended amount. The dietary structure of children was undiversified, which limited the intake of multiple nutrients. Therefore, comprehensive nutrition rather than sole selenium intake should be the primary concern in the future.
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http://dx.doi.org/10.1016/j.envint.2022.107255DOI Listing
April 2022

Circ_0000514 promotes the malignant biological behaviors of non-small cell lung cancer cells by modulating miR-330-5p and HMGA2.

Pathol Res Pract 2022 Apr 26;235:153913. Epub 2022 Apr 26.

Department of Respiratory and Critical Care Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.

Background: Circular RNA 0000514 (circ_0000514) has a modulatory function in the progression of several cancers, but its role and mechanism in non-small cell lung cancer (NSCLC) are obscure.

Methods: The relative expressions of circ_0000514, microRNA-330-5p (miR-330-5p) and high mobility group AT-hook 2 (HMGA2) mRNA in 67 cases of NSCLC and adjacent lung tissues were examined by quantitative real-time polymerase chain reaction (qRT-PCR). The changes in cell phenotypes were examined by cell counting kit-8 (CCK-8), 5-Ethynyl-2'-deoxyuridine (EdU), Transwell and flow cytometry assays, respectively. Bioinformatics analysis, dual-luciferase reporter gene experiment and RNA immunoprecipitation (RIP) experiment were adopted to predict and validate the relationship between circ_0000514 and miR-330-5p, and miR-330-5p and HMGA2 3'UTR, respectively. In addition, Western blot was performed to examine the effects of circ_0000514 overexpression or knockdown on HMGA2 protein expression.

Results: Circ_0000514 expression was up-regulated in NSCLC tissues. Circ_0000514 significantly facilitated the growth, cell cycle progression, metastatic potential, and repressed the apoptosis of NSCLC cells. Circ_0000514 was identified as a molecular sponge to decoy miR-330-5p, and HMGA2 was proven to be a target gene of miR-330-5p. Moreover, circ_0000514 positively regulated HMGA2 expression, and its biological effect was dependent on miR-330-5p/HMGA2 axis.

Conclusion: Circ_0000514 is an oncogenic circRNA in NSCLC progression, and it modulates miR-330-5p/HMGA2 axis.
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http://dx.doi.org/10.1016/j.prp.2022.153913DOI Listing
April 2022

Elevation of N-acetyltransferase 10 in hippocampal neurons mediates depression- and anxiety-like behaviors.

Brain Res Bull 2022 May 10;185:91-98. Epub 2022 May 10.

Neuroscience Research Center, Institute of Medical and Health Science, Hebei Medical University, Shijiazhuang 050017, China; Department of Biochemistry and Molecular Biology, The Key Laboratory of Neural and Vascular Biology, Ministry of Education of China, Hebei Medical University, Shijiazhuang 050017, China. Electronic address:

Major depressive disorder (MDD) is one of the most debilitating and severe mental diseases globally. Increasing evidence has shown that epigenetics is critical for understanding brain function and brain disorders, including MDD. N-acetyltransferase 10 (NAT10), acting on histones, mRNA and other substrates, has been reported to be involved in epigenetic events, including histone acetylation and mRNA modifications. NAT10 is highly expressed in the brain. However, the potential effects of NAT10 on MDD are still unknown. Here, we exploited chronic mild stress (CMS) to induce anxiety- and depression-like behaviors in mice and found that the expression of NAT10 in the mouse hippocampus was upregulated after CMS treatment. Inhibition of NAT10 by pharmacological methods produced anxiolytic- and antidepressant-like effects. Neuron-specific overexpression of NAT10 in the hippocampus resulted in anxiety- and depression-like behaviors, accompanied by higher SIRT1 protein levels, and lower dendritic spine densities. Overall, it was found that elevation of NAT10 in hippocampal neurons is involved in the occurrence of anxiety- and depression-like behaviors, suggesting that NAT10 could be a potential new target for developing anxiolytics and antidepressants.
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http://dx.doi.org/10.1016/j.brainresbull.2022.05.004DOI Listing
May 2022

Scalable processing for realizing 21.7%-efficient all-perovskite tandem solar modules.

Science 2022 05 12;376(6594):762-767. Epub 2022 May 12.

National Laboratory of Solid State Microstructures, Jiangsu Key Laboratory of Artificial Functional Materials, College of Engineering and Applied Sciences, Frontiers Science Center for Critical Earth Material Cycling, Nanjing University, Nanjing 210023, China.

Challenges in fabricating all-perovskite tandem solar cells as modules rather than as single-junction configurations include growing high-quality wide-bandgap perovskites and mitigating irreversible degradation caused by halide and metal interdiffusion at the interconnecting contacts. We demonstrate efficient all-perovskite tandem solar modules using scalable fabrication techniques. By systematically tuning the cesium ratio of a methylammonium-free 1.8-electron volt mixed-halide perovskite, we improve the homogeneity of crystallization for blade-coated films over large areas. An electrically conductive conformal "diffusion barrier" is introduced between interconnecting subcells to improve the power conversion efficiency (PCE) and stability of all-perovskite tandem solar modules. Our tandem modules achieve a certified PCE of 21.7% with an aperture area of 20 square centimeters and retain 75% of their initial efficiency after 500 hours of continuous operation under simulated 1-sun illumination.
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http://dx.doi.org/10.1126/science.abn7696DOI Listing
May 2022

Expression, Regulation, and Role of an Oligopeptide Transporter: PEPT1 in Tumors.

Curr Med Chem 2022 ;29(9):1596-1605

Central Laboratory, Tianjin Medical University General Hospital, Tianjin, 300052, China.

PEPT1 is a vital member of the proton-dependent oligopeptide transporters family (POTs). Many studies have confirmed that PEPT1 plays a critical role in the absorption of dipeptides, tripeptides, and pseudopeptides in the intestinal tract. In recent years, several studies have found that PEPT1 is highly expressed in malignant tumor tissues and cells. The abnormal expression of PEPT1 in tumors may be closely related to the progress of tumors, and hence, could be considered as a potential molecular biomarker for the diagnosis, treatment, and prognosis in malignant tumors. Furthermore, PEPT1 can be used to mediate the targeted delivery of anti-tumor drugs. Herein, the expression, regulation, and role of PEPT1 in tumors in recent years have been reviewed.
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http://dx.doi.org/10.2174/0929867328666210707170214DOI Listing
January 2022

Natural variation in the bZIP68 promoter modulates cold tolerance and was targeted during maize domestication.

Plant Cell 2022 May 11. Epub 2022 May 11.

State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, Center for Crop Functional Genomics and Molecular Breeding, China Agricultural University, Beijing 100193, China.

Maize (Zea mays) originated in tropical areas and is thus susceptible to low temperatures, which pose a major threat to maize production. Our understanding of the molecular basis of cold tolerance in maize is limited. Here, we identified bZIP68, a basic leucine zipper (bZIP) transcription factor, as a negative regulator of cold tolerance in maize. Transcriptome analysis revealed that bZIP68 represses the cold-induced expression of DREB1 transcription factor genes. The stability and transcriptional activity of bZIP68 are controlled by its phosphorylation at the conserved Ser250 residue under cold stress. Furthermore, we demonstrated that the bZIP68 locus was a target of selection during early domestication. A 358-bp insertion/deletion (Indel-972) polymorphism in the bZIP68 promoter has a significant effect on the differential expression of bZIP68 between maize and its wild ancestor teosinte. This study thus uncovered an evolutionary cis-regulatory variant that could be used to improve cold tolerance in maize.
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http://dx.doi.org/10.1093/plcell/koac137DOI Listing
May 2022

[Effects of drilling waste combined with exogenous organic matter on physicochemical properties of aeolian sandy soil and growth of spinach].

Ying Yong Sheng Tai Xue Bao 2022 Apr;33(4):887-893

State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling 712100, Shaanxi, China.

An indoor potted experiment was carried out to study the application effects of water-based waste drilling mud (WBDM) and drill cuttings combined with humic acid and woody peat in aeolian sandy soil.We analyze their effects on the growth of spinach, in order to provide a reference for the resource utilization of drilling waste and the improvement of aeolian sandy soil. A total of three individual experiments were set up: 4 treatments in the basic group, taking aeolian sandy soil as the control (CK), mixing aeolian sandy soil and drill cuttings at a mass ratio of 1:1, then added with 0, 2%, and 4% WBDM (dry basis) respectively. The basic group treatment was added with 1% humic acid, 1% humic acid+3% woody peat mixture respectively, as the humic acid group and woody peat group. The results showed that in the basic group, drilling waste application significantly increased soil organic matter content (SOM), pH and electrical conductivity, the 2% WBDM significantly promoted the growth of spinach. Humic acid and woody peat reduced soil pH and increased SOM content and electrical conductivity. Plant growth indicators such as plant height, total leaf area, fresh weight and dry weight of the humic acid group were higher than the corresponding treatments in the basic group. The mixed addition of humic acid and woody peat did not affect plant growth. The treatment with the best performance was to add 2% WBDM to the humic acid group, and the SOM was 13.1 g·kg, spinach's plant height, total leaf area, fresh weight and dry weight significantly increased by 49.7%, 93.4%, 83.3%, and 34.6% than CK after 40 days of sowing. The application of drilling waste with organic matter could significantly increase SOM content and promote spinach growth. Aeolian sandy soil + drill cuttings + WBDM (2%) + humic acid (1%) was the best combination mode.
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http://dx.doi.org/10.13287/j.1001-9332.202204.024DOI Listing
April 2022

Customized protective visors enabled by closed loop controlled 4D printing.

Sci Rep 2022 May 9;12(1):7566. Epub 2022 May 9.

Department of Machine Design, KTH Royal Institute of Technology, 10044, Stockholm, Sweden.

The COVID-19 pandemic makes protective visors important for protecting people in close contacts. However, the production of visors cannot be increased greatly in a short time, especially at the beginning of the pandemic. The 3D printing community contributed largely in fabricating the visor frames using the rapid and adaptive manufacturing ability. While there are many open source designs of face visors for affordable 3D printers, all these designs fabricate mono-sized frames without considering diverse users' dimensions. Here, a new method of visor post-processing technology enabled by closed loop controlled 4D printing is proposed. The new process can further deform the printed visor to any customized size for a more comfortable user experience. FEM analysis of the customized visor also shows consistent wearing experience in different circumstances compared with the old visor design. The fabrication precision and time cost of the method is studied experimentally. A case study regarding the reducing, reusing and recycling (3R) of customized visors in classrooms is proposed to enable the customized visors manufactured in a more sustainable way.
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http://dx.doi.org/10.1038/s41598-022-11629-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9082988PMC
May 2022

The emerging roles of N6-methyladenosine (m6A)-modified long non-coding RNAs in human cancers.

Cell Death Discov 2022 May 9;8(1):255. Epub 2022 May 9.

The School and Hospital of Stomatology, Tianjin Medical University, Qixiangtai Road, No. 12, Tianjin, 300070, P.R. China.

N-methyladenosine (mA) epitranscriptional modifications widely exist in RNA, which play critical roles in RNA metabolism and biogenesis processes. Long non-coding RNAs (lncRNAs) are class of non-coding RNAs longer than 200 nucleotides without protein-coding ability. LncRNAs participate in a large number of vital biological progressions. With the great improvement of molecular biology, mA and lncRNAs are attracting more attention from researchers and scholars. In this review, we overview the current status of mA and lncRNAs based on the latest research, and propose some viewpoints for future research perspectives.
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http://dx.doi.org/10.1038/s41420-022-01050-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9085772PMC
May 2022

Growth Hormone Is Beneficial for Induction of Spermatogenesis in Adult Patients With Congenital Combined Pituitary Hormone Deficiency.

Front Endocrinol (Lausanne) 2022 22;13:868047. Epub 2022 Apr 22.

National Health Commission (NHC) Key Laboratory of Endocrinology, Department of Endocrinology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.

Background: Gonadotropins are effective in inducing spermatogenesis in patients with congenital combined pituitary hormone deficiency (CCPHD). Data on recombinant human growth hormone(rhGH) adjuvant treatment to improve gonadotropin-induced spermatogenesis are limited.

Design And Setting: This retrospective study included 60 male patients with CCPHD on a relatively large case series in a single center from mainland China. Twenty-nine patients who received gonadotropin therapy alone were defined as the Gn group, while 31 patients treated with a combination of rhGH and gonadotropins were defined as GH/Gn group.

Results: Spermatogenesis rate was 96.77% (30/31) and 62.07% (18/29) in the GH/Gn and Gn group, respectively (P < 0.001). The time for initial sperm appearance in the GH/Gn group was shorter than in the Gn group (14 versus 23 months, P < 0.001). A higher level of serum testosterone was achieved in the GH/Gn group than in the Gn group (4.79 versus 3.38 ng/mL, P = 0.026). After adjustment for potential confounders, rhGH supplementation was an independent beneficial factor on spermatogenesis (HR = 2.294, 95% CI: 1.143-4.604, P = 0.019).

Conclusions: rhGH induces earlier spermatogenesis in patients with CCPHD, which encourages the co-treatment with rhGH and gonadotropins in CCPHD patients.
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http://dx.doi.org/10.3389/fendo.2022.868047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9074387PMC
May 2022

OSCA1 is an osmotic specific sensor: a method to distinguish Ca -mediated osmotic and ionic perception.

New Phytol 2022 May 8. Epub 2022 May 8.

Department of Biology, Duke University, Durham, NC, 27708, USA.

Genetic mutants defective in stimulus-induced Ca increases have been gradually isolated, allowing the identification of cell-surface sensors/receptors, such as osmosensor OSCA1. However, how to determine the Ca -signaling specificity to various stimuli in these mutants remains challengeable. For instance, less is known about the exact selectivity between osmotic and ionic stresses in the osca1 mutant. Here, we have developed a method to distinguish the osmotic and ionic effects by analyzing Ca increases, and demonstrated that osca1 is impaired primarily in Ca increases induced by the osmotic but not ionic stress. We recorded Ca increases induced by sorbitol (osmotic effect, OE) and NaCl/CaCl (OE + ionic effect, IE) in Arabidopsis wild-type and osca1 seedlings. We assumed the NaCl/CaCl total effect (TE) = OE + IE, then developed procedures for Ca imaging, image analysis, and mathematic fitting/modeling, and found osca1 defects mainly in OE. The osmotic specificity of osca1 suggests that osmotic and ionic perceptions are independent. The precise estimation of these two stress effects is applicable not only to new Ca -signaling mutants with distinct stimulus specificity but also the complex Ca -signaling crosstalk among multiple concurrent stresses that occur naturally, and will enable us to specifically fine tune multiple signal pathways to improve crop yields.
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http://dx.doi.org/10.1111/nph.18217DOI Listing
May 2022

Genomics and proteomics of Apis mellifera filamentous virus isolated from honeybees in China.

Virol Sin 2022 Feb 25. Epub 2022 Feb 25.

Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, 410205, China. Electronic address:

Apis mellifera filamentous virus (AmFV) is a large DNA virus that is endemic in honeybee colonies. The genome sequence of the AmFV Swiss isolate (AmFV CH-C05) has been reported, but so far very few molecular studies have been conducted on this virus. In this study, we isolated and purified AmFV (AmFV CN) from Chinese honeybee (Apis mellifera) colonies and elucidated its genomics and proteomics. Electron microscopy showed ovoid purified virions with dimensions of 300-500 ​× ​210-285 ​nm, wrapping a 3165 ​× ​40 ​nm filamentous nucleocapsid in three figure-eight loops. Unlike AmFV CH-C05, which was reported to have a circular genome, our data suggest that AmFV CN has a linear genome of approximately 493 ​kb. A total of 197 ORFs were identified, among which 36 putative genes including 18 baculoviral homologs were annotated. The overall nucleotide similarity between the CN and CH-C05 isolates was 96.9%. Several ORFs were newly annotated in AmFV CN, including homologs of per os infectivity factor 4 (PIF4) and a putative integrase. Phylogenomic analysis placed AmFVs on a separate branch within the newly proposed virus class Naldaviricetes. Proteomic analysis revealed 47 AmFV virion-associated proteins, of which 14 had over 50% sequence coverage, suggesting that they are likely to be main structural proteins. In addition, all six of the annotated PIFs (PIF-0-5) were identified by proteomics, suggesting that they may function as entry factors in AmFV infection. This study provides fundamental information regarding the molecular biology of AmFV.
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http://dx.doi.org/10.1016/j.virs.2022.02.007DOI Listing
February 2022

The genome of the recretohalophyte Limonium bicolor provides insights into salt gland development and salinity adaptation during terrestrial evolution.

Mol Plant 2022 May 5. Epub 2022 May 5.

Shandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Ji'nan, Shandong, China. Electronic address:

Halophytes have evolved specialized strategies to cope with high salinity. The extreme halophyte sea lavender (Limonium bicolor) lacks trichomes but possesses salt glands on its epidermis, which can excrete harmful ions such as sodium to avoid salt damage. Here, we report a high-quality, 2.92-Gb chromosome-scale L. bicolor genome assembly using a combination of Illumina short reads, single-molecule real-time long reads, chromosome conformation capture (Hi-C) data, and Bionano genome maps, which have greatly enriched genomic information of recretohalophyte with multicellular salt glands. Although the L. bicolor genome possesses genes showing similarity to trichome fate genes from Arabidopsis (Arabidopsis thaliana), it lacks homologs of the decision fate genes GLABRA3, ENHANCER OF GLABRA3, GLABRA2, TRANSPARENT TESTA GLABRA2, and SIAMESE, providing a molecular explanation for the absence of trichomes in this species. We identified key genes (LbHLH and LbTTG1) controlling salt gland development among classical trichome homologous genes and confirmed their roles by showing that their mutation markedly disrupted salt gland initiation, salt secretion and salt tolerance, thus offering the genetic support for the long-standing hypothesis that salt glands and trichomes may share a common origin. In addition, a whole-genome duplication event occurred in the L. bicolor genome after its divergence from Tartary buckwheat, which may have contributed to its adaptation to high salinity. The L. bicolor genome resource gives profound insights into plant salt tolerance mechanisms that should facilitate the engineering of salt-tolerant crops.
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http://dx.doi.org/10.1016/j.molp.2022.04.011DOI Listing
May 2022

Exosomal AFAP1-AS1 binds to microRNA-15a-5p to promote the proliferation, migration, and invasion of ectopic endometrial stromal cells in endometriosis.

Reprod Biol Endocrinol 2022 May 5;20(1):77. Epub 2022 May 5.

Department of Obstetrics and Gynecology, The Second Xiangya Hospital, Central South University, NO.139 Renmin Road, Changsha, Hunan, 410000, PR China.

Background: Endometriosis (EMS) remains a major challenge to reproductive health due to multifactorial etiology, disease heterogeneity, and the lack of appropriate diagnostic markers and treatment. Eexosome (Exo) has become a major factor in progression of a variety of diseases. However, the mechanisms directing their role in the pathophysiology of EMS are ill-defined. Here, we aimed to investigate the clinical implications of actin filament associated protein 1-Antisense RNA 1 (AFAP1-AS1) in EMS.

Methods: Bioinformatics analysis was used to predict the expression and interaction of AFAP1-AS1, miR-15a-5p and BCL9 in EMS, and dual luciferase reporter assay was used to verify the targeted relationship of AFAP1-AS1, miR-15a-5p, and BCL9. The Exo from endometrial stromal cells (ESCs) was isolated and characterized by transmission electron microscopy (TEM) and Nanoparticle tracking analysis (NTA). Exosome uptake studies were performed. For in vitro assay, ectopic ESCs (EcESCs) proliferation, migration, and invasion were assessed by CCK-8 and Transwell assays. In vivo assay was performed by establishment of EMS mice to validate the result derived from in vitro assay.

Results: The Exo was successfully isolated from ESCs and we observed high expression of AFAP1-AS1 and BCL9 but low expression of miR-15a-5p in EMS. Moreover, Exo derived from EcESCs could deliver AFAP1-AS1 to EcESCs and thus promoting proliferation, migration, and invasion of ESCs. AFAP1-AS1 bound to BCL9, which was targeted by miR-15a-5p in EMS. In vivo experiments in nude mice revealed that inhibition of Exosomal AFAP1-AS1 suppressed migration and invasion of EcESCs through miR-15a-5p/BCL9.

Conclusions: Collectively, these findings suggested that ESCs-derived Exo carrying AFAP1-AS1 contributed to EMS pathogenesis. This study might help us realize the etiology of EMS and improve the treatment of the related complications.
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http://dx.doi.org/10.1186/s12958-022-00942-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9069797PMC
May 2022

Bioinformatic Analyzes of the Association Between Upregulated Expression of Gene -Induced Gene Mutation and Prognosis of Cervical Cancer.

Front Med (Lausanne) 2022 18;9:815450. Epub 2022 Apr 18.

Department of Obstetrics and Gynecology, The Second XIANGYA Hospital of Central South University, Changsha, China.

Globally, cervical cancer (CC) is the most common malignant tumor of the female reproductive system and its incidence is only second after breast cancer. Although screening and advanced treatment strategies have improved the rates of survival, some patients with CC still die due to metastasis and drug resistance. It is considered that cancer is driven by somatic mutations, such as single nucleotide, small insertions/deletions, copy number, and structural variations, as well as epigenetic changes. Previous studies have shown that cervical intraepithelial neoplasia is associated with copy number variants (CNVs) and/or mutations in cancer-related genes. Further, CC is also related to genetic mutations. The present study analyzed the data on somatic mutations of cervical squamous cell carcinoma (CESC) in the Cancer Genome Atlas database. It was evident that the Apolipoprotein B mRNA editing enzyme-catalyzed polypeptide-like ()-related mutation of the gene can upregulate the expression of the gene and ultimately lead to poor prognosis for patients with CC. Therefore, the findings of the current study provide a new direction for future treatment of CC.
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http://dx.doi.org/10.3389/fmed.2022.815450DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9058067PMC
April 2022

Predictors of Maternal Death Among Women With Pulmonary Hypertension in China From 2012 to 2020: A Retrospective Single-Center Study.

Front Cardiovasc Med 2022 18;9:814557. Epub 2022 Apr 18.

Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Background: Previous studies have suggested that pregnant women with pulmonary hypertension (PH) have high maternal mortality. However, indexes or factors that can predict maternal death are lacking.

Methods: We retrospectively reviewed pregnant women with PH admitted for delivery from 2012 to 2020 and followed them for over 6 months. The patients were divided into two groups according to 10-day survival status after delivery. Predictive models and predictors for maternal death were identified using four machine learning algorithms: naïve Bayes, random forest, gradient boosting decision tree (GBDT), and support vector machine.

Results: A total of 299 patients were included. The most frequent PH classifications were Group 1 PH (73.9%) and Group 2 PH (23.7%). The mortality within 10 days after delivery was 9.4% and higher in Group 1 PH than in the other PH groups (11.7 vs. 2.6%, = 0.016). We identified 17 predictors, each with a -value < 0.05 by univariable analysis, that were associated with an increased risk of death, and the most notable were pulmonary artery systolic pressure (PASP), platelet count, red cell distribution width, N-terminal brain natriuretic peptide (NT-proBNP), and albumin (all < 0.01). Four prediction models were established using the candidate variables, and the GBDT model showed the best performance (F1-score = 66.7%, area under the curve = 0.93). Feature importance showed that the three most important predictors were NT-proBNP, PASP, and albumin.

Conclusion: Mortality remained high, particularly in Group 1 PH. Our study shows that NT-proBNP, PASP, and albumin are the most important predictors of maternal death in the GBDT model. These findings may help clinicians provide better advice regarding fertility for women with PH.
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http://dx.doi.org/10.3389/fcvm.2022.814557DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9058072PMC
April 2022

Whole Genome Sequencing and Biological Characteristics of Two Strains of Porcine Escherichia coli Isolated from Saba Pigs.

Curr Microbiol 2022 May 5;79(6):182. Epub 2022 May 5.

College of Veterinary Medicine, Yunnan Agricultural University, Kunming, China.

Escherichia coli (E. coli) is an important pathogen that causes diarrhea and death in piglets. In this work, whole genome sequencing of two E. coli strains (ZB-1, ZWW-1) isolated from Saba pigs. And focus on the relationship between drug resistance, pathogenic phenotype and genotype of the two strains. This study analyzed the drug susceptibility of the two strains. The LD50 values, tissue bacterial load and intestinal pathological changes in mice infected with the two strains. The differences in gene functions such as drug resistance, virulence, and unique genes between the two strains, as well as the genetic evolutionary relationship of housekeeping genes were analyzed. The results showed that the two strains had the same resistance phenotype to most drugs. The LD50 value, tissue load, and pathological changes in mice infected with strain ZB-1 revealed that this strain was more virulent and pathogenic than strain ZWW-1. In addition, the housekeeping genes contained in the two strains are in the same large branch as E. coli of different species, and the genetic evolution is stable. All of them carry EPEC-type strain-specific virulence genes escV and ent, indicating that they are all new members of EPEC-type strains. This study laid the foundation for understanding the genetic background and biological characteristics of E. coli from Saba pigs.
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http://dx.doi.org/10.1007/s00284-022-02873-xDOI Listing
May 2022

Corrigendum: Phylogeny and Taxonomy on Cryptic Species of Forked Ferns of Asia.

Front Plant Sci 2022 12;13:888725. Epub 2022 Apr 12.

Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, The National Orchid Conservation Center of China and The Orchid Conservation and Research Center of Shenzhen, Shenzhen, China.

[This corrects the article DOI: 10.3389/fpls.2021.748562.].
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http://dx.doi.org/10.3389/fpls.2022.888725DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9040669PMC
April 2022

Long-Term Risk of Death From Heart Disease Among Breast Cancer Patients.

Front Cardiovasc Med 2022 13;9:784409. Epub 2022 Apr 13.

Department of Radiation Oncology, University Hospitals Seidman Cancer Center, Case Western Reserve School of Medicine, Cleveland, OH, United States.

Background: Most breast cancer patients die of non-cancer causes. The risk of death from heart disease, a leading cause of death, is unknown. The aim of this study is to characterize the long-term risk of fatal heart disease in breast cancer patients.

Methods: This retrospective study used the Surveillance, Epidemiology, and End Results (SEER) database. Standard mortality ratios (SMR) were calculated for breast cancer patients diagnosed from 1992 to 2014. Patients were stratified by receipt of radiotherapy and/or chemotherapy, disease laterality, and diagnosis era. Hazard ratios (HRs) and odds ratios (ORs) were calculated to compare the risk of death from heart disease among other breast cancer patients.

Results: There were 1,059,048 patients diagnosed with breast cancer from 1992 to 2014, of which 47,872 (4.6%) died from heart disease. The SMR for death from heart disease at 10+ years for patients who received only radiotherapy was 2.92 (95% CI 2.81-3.04, < 0.001) and in patients who received only chemotherapy was 5.05 (95% CI 4.57-5.55, < 0.001). There was no statistically significant difference in SMR for death from heart disease for left-sided vs. right-sided disease. At 10+ years, heart disease made up 28% of deaths from non-primary cancer. HRs and ORs showed that the risk of death from heart disease was highest in patients older than 70 years of age and with longer follow-up.

Conclusion: The risk of fatal heart disease was highest in older breast cancer patients with longer follow-up (i.e., >5-10 years) and who received chemotherapy. These patients should be referred to cardio-oncology clinics to mitigate this risk.
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http://dx.doi.org/10.3389/fcvm.2022.784409DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9043135PMC
April 2022

Changes in Rats' Gut Microbiota Composition Caused by Induced Chronic Myocardial Infarction Lead to Depression-Like Behavior.

Front Microbiol 2021 14;12:641084. Epub 2022 Apr 14.

Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, China.

Depression is common among patients who have chronic myocardial infarction (CMI). Despite their frequency, depression and CMI are bidirectional related conditions, each is a risk for the other, and they often co-exist, suggesting shared or interacting pathomechanisms. Accumulating data revealed the effects of gut microbiota in terms of regulating depression the gut-brain axis. Thus, we investigated the role of gut microbial dysbiosis in CMI-induced depression-like behavior. Hierarchical cluster analysis of sucrose preference test (SPT) results was adopted to classify the CMI rats into depression-like behavior (CMI + Dep) or non-depression-like behavior (CMI + Non-Dep) phenotypes. First, 16S ribosomal RNA sequencing analysis showed both β-diversity and relative abundance of several gut bacteria significantly differed between the CMI + Dep and CMI + Non-Dep rats. Next, transplantation of fecal microbiota from CMI + Dep rats visibly altered the relative abundance of gut microbiota and also induced depression-like behavior in the antibiotics-treated pseudo-germ-free rats. In conclusion, these findings suggested that dysbiosis in gut microbial composition contributed to the onset of CMI-induced depression-like behavior and that exogenous regulation of gut microbiota composition could be a potential therapeutic strategy for CMI and related depression-like behavior.
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http://dx.doi.org/10.3389/fmicb.2021.641084DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048826PMC
April 2022

Rab22a promotes the proliferation, migration, and invasion of lung adenocarcinoma via up-regulating PI3K/Akt/mTOR signaling pathway.

Exp Cell Res 2022 Jul 27;416(2):113179. Epub 2022 Apr 27.

Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang, China. Electronic address:

Rab22a, a member of the proto-oncogene RAS family, belongs to the Rab5 subfamily. It participates in early endosome formation and regulates vesicle trafficking. The relationship between Rab22a and tumorigenesis remains elusive. In non-small cell lung cancer specimens, immunohistochemical staining showed consistently high expression of Rab22a in lung adenocarcinoma, but not in squamous cell carcinoma. In lung adenocarcinoma cell lines, A549 and H1299, transfection with Rab22a significantly promoted cell proliferation, migration, and invasion, whereas interference with Rab22a specific siRNA significantly inhibited the above capacities. Transfection with Rab22a also up-regulated the phosphorylation levels of core effector proteins on the PI3K/Akt/mTOR pathway. The Co-IP assay further confirmed the interaction between Rab22a and PI3Kp85α, the core regulatory subunit of PI3K. Application of rapamycin, the mTOR inhibitor, significantly reduced the upregulation of the proliferation, migration, and invasion abilities of lung adenocarcinoma cells transfected with Rab22a. These results suggest that Rab22a can promote the malignant phenotype of lung adenocarcinoma by upregulating the PI3K/Akt/mTOR signaling pathway, and may function as a potential anti-tumor therapeutic target.
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http://dx.doi.org/10.1016/j.yexcr.2022.113179DOI Listing
July 2022

JMJD2C mediates the MDM2/p53/IL5RA axis to promote CDDP resistance in uveal melanoma.

Cell Death Discov 2022 Apr 25;8(1):227. Epub 2022 Apr 25.

Department of Ophthalmology, The Second Hospital of Jilin University, Changchun, 130000, People's Republic of China.

Chemotherapy resistance poses an obstacle for effective treatment of uveal melanoma. In this study, we aim to investigate the effects of jumonji domain containing 2C (JMJD2C)-mediated mouse double minute-2 homolog (MDM2)/p53/interleukin 5 receptor subunit alpha (IL5RA) axis on cisplatin (CDDP) resistance in uveal melanoma. RT-qPCR and Western blot assay were performed to determine their expression patterns in uveal melanoma cell line (MUM-2B) and CDDP-resistant cell line (MUM-2B/CDDP). The enrichment of H3K9me3 in MDM2 promoter region was examined by ChIP, and the binding between p53 and ubiquitin in MUM-2B cells testified by co-IP assay. Following overexpression or silencing of JMJD2C/MDM2/p53/IL5RA, the 50% concentration of inhibition (IC50) and the biological characteristics of MUM-2B and MUM-2B/CDDP cells were examined using CCK-8 assay, SA-β-gal staining, fluorescence-activated cell sorting analysis, and Transwell assay. Finally, the tumorigenicity of transplanted MUM-2B and MUM-2B/CDDP cells in nude mice was assessed. JMJD2C was documented to be highly expressed in uveal melanoma cells, promoting the CDDP resistance. Histone demethylase JMJD2C removed the H3K9me3 modification of MDM2 promoter, which promoted the expression of MDM2. MDM2 enhanced the IL5RA expression through stimulating the ubiquitination and degradation of p53, thus inducing CDDP resistance of uveal melanoma cells. Furthermore, the results of in vivo experiments revealed that JMJD2C mediated the MDM2/p53/IL5RA axis to expedite the growth of uveal melanoma and augment the CDDP resistance. Taken together, JMJD2C can induce histone demethylation to upregulate MDM2, thereby ubiquitinating p53 and upregulating IL5RA. As a consequence, CDDP resistance in uveal melanoma is ultimately accelerated.
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http://dx.doi.org/10.1038/s41420-022-00949-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039082PMC
April 2022

The Status of Selenium and Zinc in the Urine of Children From Endemic Areas of Kashin-Beck Disease Over Three Consecutive Years.

Front Nutr 2022 8;9:862639. Epub 2022 Apr 8.

Key Laboratory of Trace Elements and Endemic Diseases, Collaborative Innovation Center of Endemic Disease and Health Promotion for Silk Road Region, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, China.

Selenium deficiency is one of the main risk factors for Kashin-Beck disease (KBD). This study aimed to detect the status of selenium and zinc in the urine of children from endemic areas of KBD over three consecutive years and to evaluate whether selenium and zinc levels in children in Shaanxi Province remain normal after stopping selenium supplementation. The samples of urine were collected in consecutive years (2017-2019) to detect selenium content by hydride generation atomic fluorescence spectrometry (HGAFS) and to detect zinc content by atomic absorption spectrophotometry (AAS). Generalized estimation equation (GEE) analysis was integrated to assess the comprehensive nutritional status and dietary structure of children. Data were processed in duplicate and analyzed by SPSS 18.0. This study included 30 X-ray-positive KBD cases and 123 healthy children aged 7-12 years. A total of 424 urine and 137 hair samples were collected over three consecutive years for selenium determination. The mean value of urinary selenium in all subjects was 6.86 μg/l (2017), 8.26 μg/l (2018), and 4.04 μg/l (2019), and the mean value of urinary zinc in all subjects was 0.36 mg/l (2017), 0.39 mg/l (2018), and 0.31 mg/l (2019) for the three consecutive years of 2017-2019. The mean values of urinary selenium were 6.56 and 6.94 μg/l (2017), 8.69 and 8.14 μg/l (2018), and 4.57 and 3.90 μg/l (2019) in the KBD-X and normal groups, respectively; and the mean value of urinary zinc were 0.38 and 0.35 mg/l (2017), 0.41 and 0.39 mg/l (2018), and 0.43 and 0.28 mg/l (2019) in the KBD-X and normal groups, respectively. The mean value of hair selenium in 137 subjects was 275.08 μg/kg and the mean values of hair selenium were 267.48 and 276.61 μg/kg in the KBD-X group and normal group, respectively. The level of selenium/zinc showed a trend of increasing first and then decreasing during the three consecutive years. The level of selenium in all subjects from the endemic areas was lower than normal, which reminds us to monitor the state of KBD constantly and adjust selenium salt supplementation in accordance with the changes in the KBD state.
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http://dx.doi.org/10.3389/fnut.2022.862639DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9033266PMC
April 2022

The Mechanism of Lipopolysaccharide Escaping the Intestinal Barrier in Fed a High-Fat Diet.

Front Nutr 2022 7;9:853409. Epub 2022 Apr 7.

Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.

With the popularity of western food characterized by excessive fat and sugars, obesity has currently been a public health issue. Low-grade chronic inflammation accompanied by obesity increases the risk of multiple epidemics such as diabetes, cancer and cardiovascular diseases. Here, we show that feeding with a high-fat diet (HFD) drives obesity-related chronic inflammation and the penetration of lipopolysaccharide (LPS). Interference with antibiotics inhibits the produce of LPS and this alleviates the sustained release of pro-inflammatory factors induced by HFD. LPS penetration is attributed to weakened intestinal mucus barrier after high-fat exposure. Mechanically, the consumption of HFD inhibits the secretion of mucin 2 (MUC2) due to the induction of endoplasmic reticulum stress mediated by the inositol-requiring enzyme 1 (IRE1) /X box-binding protein 1 (XBP1) pathway in goblet cells. Furthermore, excessive lipid exacerbates the leakage of LPS across the intestinal epithelial cell barrier via the transcellular pathway. Mechanically, lipid increases the internalization of LPS in intestinal epithelial cells depending on the activation of fatty acid translocase (FAT/CD36). These results demonstrate that HFD causes the penetration of LPS due to the weakened intestinal mucosal barrier and the assistance of CD36.
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http://dx.doi.org/10.3389/fnut.2022.853409DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023073PMC
April 2022

Chondroitin sulfate E alleviates β-amyloid toxicity in transgenic Caenorhabditis elegans by inhibiting its aggregation.

Int J Biol Macromol 2022 Jun 21;209(Pt A):1280-1287. Epub 2022 Apr 21.

School of Rehabilitation Medicine, Binzhou Medical University, Yantai 264003, China. Electronic address:

Chondroitin sulfate E (CS-E), which is characterized by oversulfated disaccharide units, has been shown to regulate neuronal adhesion, neurite outgrowth and exert neuroprotective effects. In view of these findings, here we investigated the anti-Alzheimer's disease (AD) activities of CSE by using transgenic Caenorhabditis elegans model of Alzheimer's disease. The behavioral experiments demonstrated that CSE at the concentration of 1 mg/mL significantly delayed the worm paralysis caused by Aβ aggregation as compared with control group. Western blot analysis revealed that the level of small oligomers in the transgenic C. elegans was significantly reduced upon treatment with CSE. The number of Aβ plaque deposits in transgenic worm was significantly decreased. In addition, CSE also protected the worms from oxidative stress and rescued chemotaxis dysfunction in transgenic strain CL2355. Taken together, these data suggested that CSE could protect against Aβ-induced toxicity in C. elegans. These results offer valuable evidence for the future use of CSE in the development of agents for the treatment of AD.
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http://dx.doi.org/10.1016/j.ijbiomac.2022.04.124DOI Listing
June 2022
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