Publications by authors named "W K Han"

5,671 Publications

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Multigene Panel Testing for Hereditary Cancer and Genetic Counseling.

Adv Exp Med Biol 2021 ;1187:455-471

Seoul National University Hospital, Seoul, South Korea.

As sequencing technology and information of the genomic causes for cancer development expand, multi-gene panel testing for hereditary cancer is increasing in clinical practice. In this chapter, we reviewed the application of multi-gene panel with pre-/post- testing considerations and summarized genetic counseling based on panel testing results in clinical field. In addition, we introduce multi-gene panel for hereditary cancer developed in Seoul National University Hospital.
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http://dx.doi.org/10.1007/978-981-32-9620-6_24DOI Listing
January 2021

Next-Generation Sequencing-Based Biomarkers in Breast Cancer.

Adv Exp Med Biol 2021 ;1187:323-335

Department of Surgery, Seoul National University College of Medicine, Seoul, South Korea.

For the realization of precision medicine in cancer treatment, discovery, and validation of clinically useful biomarker is the most important prerequisite. Biomarkers are needed and used for evaluation of cancer susceptibility, cancer screening (early detection), cancer subtyping, prediction of prognosis, decision of appropriate adjuvant therapy and duration of therapy, and for monitoring of recurrence. Biomarkers are also needed for decision of target therapy in metastatic cancer and monitoring of their response during follow-up. Now is the era of Next-Generation Sequencing (NGS). NGS technology can detect almost all kind of genomic changes that occur in cancer that is different from normal condition. The cost also is now reasonably low to use in routine clinical practice.This chapter will review four kinds of NGS-based biomarkers that are already being used in clinical practice although the routine use is controversial, and that are promising and under active investigation focusing on studies done in Seoul National University Hospital (SNUH).
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http://dx.doi.org/10.1007/978-981-32-9620-6_16DOI Listing
January 2021

Advances in Tumor Sampling and Sequencing in Breast Cancer and their Application in Precision Diagnostics and Therapeutics.

Adv Exp Med Biol 2021 ;1187:215-244

Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, South Korea.

Intra- and Inter-tumoral heterogeneity is one of the main hurdles in diagnosing and treating breast cancer. Selecting, sampling, and sequencing the samples appropriately provide unique opportunities in realizing precision medicine. This chapter reviews some of the past landmarks, state-of-the-art technologies, and future directions of translational research in terms of tumor sampling technologies and sequencing in breast cancer. In the state-of-the-art technologies section, the technologies are categorized in terms of scientific, precision diagnostic, and precision therapeutic tools. Finally, limitations and future directions regarding various translational research for clinical applications using these technologies will be discussed.
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http://dx.doi.org/10.1007/978-981-32-9620-6_11DOI Listing
January 2021

Fusion Genes in Breast Cancer.

Adv Exp Med Biol 2021 ;1187:147-157

Department of Surgery, Seoul National University College of Medicine, Seoul, South Korea.

Fusion gene is a hybrid form of two distinct genes resulting in broad spectrum of downstream pathway alterations. Gene fusion events are mostly from genomic rearrangements though diverse mechanisms have now been identified from in-depth analyses of next generation sequencing data. While profound level of genomic, transcriptomic driver alterations have been identified, till now, not many gene fusions are found to be the 'driver' of cancer development nor progression.ESR1 gene is a protein coding gene, encoding estrogen receptor, a transcription factor which is a key pathway in hormone sensitive breast cancers. Several hotspot mutations of this ESR1 gene have recently been associated with resistance to endocrine therapy. ESR1 gene fusion with diverse partner genes have also been identified recently, and are suggested to be acquired during previous endocrine therapy. Recurrent ESR1 gene fusions are indicative of ligand-independent hyperactivity according to 3' partner gene found among 1%~ of metastatic hormone sensitive metastatic breast cancers.
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http://dx.doi.org/10.1007/978-981-32-9620-6_7DOI Listing
January 2021

Opposing and Operated Side Electroacupuncture Generates Similar Analgesic Effects on Pain after Knee Surgery.

Evid Based Complement Alternat Med 2021 23;2021:6616886. Epub 2021 Apr 23.

Shanghai University of Traditional Chinese Medicine, 1200 Cailun Rd, Shanghai 201203, China.

The purpose of this study was to investigate whether opposing electroacupuncture (EA) could produce similar analgesic effects as operated side EA after knee surgery in rats. Sprague Dawley rats were randomly divided into the sham surgery group, and three surgery groups: opposing EA, operated side EA, and model. After surgery, compared with the sham surgery group, three kinds of pain behavior test methods (mechanical withdrawal threshold (MWT), cumulative pain score [CPS], and mechanical hypersensitivity of knee) were used to assess the pain behavior of the rats in the surgery groups. After knee surgery, the three surgery groups were intervened for three consecutive days: EA on the nonoperated side in the opposing EA group, EA on the operated side in the operated side EA group, and no intervention in the model group. It was shown that MWT was higher and CPS was lower in the two EA groups than in the model group on the first and second days after surgery. On the third day after surgery, MWT in the two EA groups was the highest among the 3 days, CPS was the lowest among the 3 days, and the number of nonvocalizations in rats also increased compared with the model group. Moreover, the MWT of the nonoperated side increased more in the opposing EA group than in the model and operated side EA groups. This indicated that both opposing EA and operated side EA methods can be used to relieve pain after knee joint surgery.
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http://dx.doi.org/10.1155/2021/6616886DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087472PMC
April 2021