Vikas Taank - Dept of Biological Sciences, Old Dominion University  - Doctoral Candidate

Vikas Taank

Dept of Biological Sciences, Old Dominion University

Doctoral Candidate

Norfolk, VA | United States

Main Specialties: Infectious Disease

ORCID logohttps://orcid.org/0000-0001-6290-4150


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Vikas Taank - Dept of Biological Sciences, Old Dominion University  - Doctoral Candidate

Vikas Taank

Introduction

I work with Human Anaplasmosis pathogen survival in arthropod host.

Primary Affiliation: Dept of Biological Sciences, Old Dominion University - Norfolk, VA , United States

Specialties:


View Vikas Taank’s Resume / CV

Education

May 2019
Old Dominion University
PhD
May 2012
Old Dominion University
Certificate
Molecular Diagnositics Certificate Course
Dec 2007
University of New Haven
MS
MS (Cellular and Molecular Biology) West Haven, CT
May 2002
Chaudhary Charan Singh University
MSc
MSc (Microbiology) Meerut, India
Dec 1999
Delhi Univeristy
BSc
BSc (Microbiology - Honors) Delhi, India

Experience

May 2018
Teaching Assistant

Publications

5Publications

614Reads

16Profile Views

20PubMed Central Citations

Ixodes scapularis Src tyrosine kinase facilitates Anaplasma phagocytophilum survival in its arthropod vector.

Ticks Tick Borne Dis 2019 06 6;10(4):838-847. Epub 2019 Apr 6.

Department of Biological Sciences, Old Dominion University, Norfolk, VA, USA; Center for Molecular Medicine, College of Sciences, Old Dominion University, Norfolk, VA, USA. Electronic address:

https://authors.elsevier.com/c/1Z4yQ,giob039O


Anaplasma phagocytophilum, the agent of human anaplasmosis, is an obligate intracellular bacterium that uses multiple survival strategies to persist in Ixodes scapularis ticks. Our previous study showed that A. phagocytophilum efficiently induced the tyrosine phosphorylation of several Ixodes proteins that includes extended phosphorylation of actin at tyrosine residue Y178. In order to identify the tyrosine kinase responsible for the A. phagocytophilum induced tyrosine phosphorylation of proteins, we combed the I. scapularis genome and identified a non-receptor Src tyrosine kinase ortholog. I. scapularis Src kinase showed high degree of amino acid sequence conservation with Dsrc from Drosophila melanogaster. We noted that at different developmental stages of I. scapularis ticks, larvae expressed significantly higher levels of src transcripts in comparison to the other stages. We found that A. phagocytophilum significantly reduced Src levels in unfed nymphs and in nymphs while blood feeding (48h during feeding) in comparison to the levels noted to relative uninfected controls. However, A. phagocytophilum increased Src levels in fully engorged larvae and nymphs (48h post feeding) and in vitro tick cells in comparison to the relative uninfected controls. Inhibition of Src kinase expression and activity by treatment with src-dsRNA or Src-inhibitor, respectively, significantly reduced A. phagocytophilum loads in ticks and tick cells. Overall, our study provides evidence for the important role of I. scapularis Src kinase in facilitating A. phagocytophilum colonization and survival in the arthropod vector.




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https://linkinghub.elsevier.com/retrieve/pii/S1877959X183038
Publisher Site
http://dx.doi.org/10.1016/j.ttbdis.2019.04.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6613393PMC
June 2019
60 Reads
2.949 Impact Factor

Characterization of tick organic anion transporting polypeptides (OATPs) upon bacterial and viral infections.

Parasit Vectors 2018 Nov 14;11(1):593. Epub 2018 Nov 14.

Department of Biological Sciences, Old Dominion University, Norfolk, VA, USA.

Background: Ixodes scapularis organic anion transporting polypeptides (OATPs) play important roles in tick-rickettsial pathogen interactions. In this report, we characterized the role of these conserved molecules in ticks infected with either Lyme disease agent Borrelia burgdorferi or tick-borne Langat virus (LGTV), a pathogen closely related to tick-borne encephalitis virus (TBEV).

Results: Quantitative real-time polymerase chain reaction analysis revealed no significant changes in oatps gene expression upon infection with B. burgdorferi in unfed ticks. Synchronous infection of unfed nymphal ticks with LGTV in vitro revealed no significant changes in oatps gene expression. However, expression of specific oatps was significantly downregulated upon LGTV infection of tick cells in vitro. Treatment of tick cells with OATP inhibitor significantly reduced LGTV loads, kynurenine amino transferase (kat), a gene involved in the production of tryptophan metabolite xanthurenic acid (XA), levels and expression of several oatps in tick cells. Furthermore, bioinformatics characterization of OATPs from some of the medically important vectors including ticks, mosquitoes and lice revealed the presence of several glycosylation, phosphorylation and myristoylation sites.

Conclusions: This study provides additional evidence on the role of arthropod OATPs in vector-intracellular pathogen interactions.

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http://dx.doi.org/10.1186/s13071-018-3160-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6236954PMC
November 2018
162 Reads
1 Citation
3.430 Impact Factor

Arthropod transcriptional activator protein-1 (AP-1) aids tick-rickettsial pathogen survival in the cold.

Sci Rep 2018 Jul 30;8(1):11409. Epub 2018 Jul 30.

Department of Biological Sciences, Old Dominion University, Norfolk, VA, USA.

Ixodes scapularis ticks transmit several pathogens to humans including rickettsial bacterium, Anaplasma phagocytophilum. Here, we report that A. phagocytophilum uses tick transcriptional activator protein-1 (AP-1) as a molecular switch in the regulation of arthropod antifreeze gene, iafgp. RNAi-mediated silencing of ap-1 expression significantly affected iafgp gene expression and A. phagocytophilum burden in ticks upon acquisition from the murine host. Gel shift assays provide evidence that both the bacterium and AP-1 influences iafgp promoter and expression. The luciferase assays revealed that a region of approximately 700?bp upstream of the antifreeze gene is sufficient for AP-1 binding to promote iafgp gene expression. Furthermore, survival assays revealed that AP-1-deficient ticks were more susceptible to cold in comparison to the mock controls. In addition, this study also indicates arthropod AP-1 as a global regulator for some of the tick genes critical for A. phagocytophilum survival in the vector. In summary, our study defines a novel mode of arthropod signaling for the survival of both rickettsial pathogen and its medically important vector in the cold.

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http://dx.doi.org/10.1038/s41598-018-29654-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6065373PMC
July 2018
1 Read
2 Citations
5.078 Impact Factor

Human rickettsial pathogen modulates arthropod organic anion transporting polypeptide and tryptophan pathway for its survival in ticks.

Sci Rep 2017 10 16;7(1):13256. Epub 2017 Oct 16.

Department of Biological Sciences, Old Dominion University, Norfolk, VA, USA.

The black-legged tick Ixodes scapularis transmits the human anaplasmosis agent, Anaplasma phagocytophilum. In this study, we show that A. phagocytophilum specifically up-regulates I. scapularis organic anion transporting polypeptide, isoatp4056 and kynurenine amino transferase (kat), a gene involved in the production of tryptophan metabolite xanthurenic acid (XA), for its survival in ticks. RNAi analysis revealed that knockdown of isoatp4056 expression had no effect on A. phagocytophilum acquisition from the murine host but affected the bacterial survival in tick cells. Knockdown of the expression of kat mRNA alone or in combination with isoatp4056 mRNA significantly affected A. phagocytophilum survival and isoatp4056 expression in tick cells. Exogenous addition of XA induces isoatp4056 expression and A. phagocytophilum burden in both tick salivary glands and tick cells. Electrophoretic mobility shift assays provide further evidence that A. phagocytophilum and XA influences isoatp4056 expression. Collectively, this study provides important novel information in understanding the interplay between molecular pathways manipulated by a rickettsial pathogen to survive in its arthropod vector.

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http://dx.doi.org/10.1038/s41598-017-13559-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5643405PMC
October 2017
186 Reads
6 Citations
5.078 Impact Factor

Ticks elicit variable fibrinogenolytic activities upon feeding on hosts with different immune backgrounds.

Sci Rep 2017 03 16;7:44593. Epub 2017 Mar 16.

Department of Biological Sciences, Old Dominion University, Norfolk, VA, USA.

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http://dx.doi.org/10.1038/srep44593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5353578PMC
March 2017
205 Reads
11 Citations
5.078 Impact Factor

Top co-authors

Girish Neelakanta
Girish Neelakanta

Yale University School of Medicine

5
Hameeda Sultana
Hameeda Sultana

Yale University School of Medicine

5
John F Anderson
John F Anderson

Yale University School of Medicine

4
Durland Fish
Durland Fish

Yale School of Public Health

2
Sucharita M Dutta
Sucharita M Dutta

University of North Dakota

1
Ashish Vora
Ashish Vora

Old Dominion University

1
Daniel E Sonenshine
Daniel E Sonenshine

Old Dominion University

1
John D Catravas
John D Catravas

Medical College of Georgia

1