Publications by authors named "Tom Aalbers"

3 Publications

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Nanoparticle Analysis by Online Comprehensive Two-Dimensional Liquid Chromatography combining Hydrodynamic Chromatography and Size-Exclusion Chromatography with Intermediate Sample Transformation.

Anal Chem 2017 09 8;89(17):9167-9174. Epub 2017 Aug 8.

Analytical-Chemistry Group, University of Amsterdam, van't Hoff Institute for Molecular Sciences , Science Park 904, 1098 XH Amsterdam, The Netherlands.

Polymeric nanoparticles have become indispensable in modern society with a wide array of applications ranging from waterborne coatings to drug-carrier-delivery systems. While a large range of techniques exist to determine a multitude of properties of these particles, relating physicochemical properties of the particle to the chemical structure of the intrinsic polymers is still challenging. A novel, highly orthogonal separation system based on comprehensive two-dimensional liquid chromatography (LC × LC) has been developed. The system combines hydrodynamic chromatography (HDC) in the first-dimension to separate the particles based on their size, with ultrahigh-performance size-exclusion chromatography (SEC) in the second dimension to separate the constituting polymer molecules according to their hydrodynamic radius for each of 80 to 100 separated fractions. A chip-based mixer is incorporated to transform the sample by dissolving the separated nanoparticles from the first-dimension online in tetrahydrofuran. The polymer bands are then focused using stationary-phase-assisted modulation to enhance sensitivity, and the water from the first-dimension eluent is largely eliminated to allow interaction-free SEC. Using the developed system, the combined two-dimensional distribution of the particle-size and the molecular-size of a mixture of various polystyrene (PS) and polyacrylate (PACR) nanoparticles has been obtained within 60 min.
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http://dx.doi.org/10.1021/acs.analchem.7b01906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5588091PMC
September 2017

Temperature control in large-internal-diameter scaffolded monolithic columns operated at ultra-high pressures.

J Chromatogr A 2015 Jul 11;1401:60-8. Epub 2015 May 11.

University of Amsterdam, van't Hoff Institute for Molecular Sciences, Analytical-Chemistry Group, Science Park 904, 1098 XH Amsterdam, The Netherlands.

Scaffolding makes it feasible to create organic-polymer monoliths in large confinements, such as wide-bore columns. By creating the scaffold from a metal good heat conductivity inside the column is obtained, which renders the relatively large columns (comparable with 4.6 mm i.d.) suitable for application under ultra-high-pressure LC conditions. It was anticipated that the metal scaffold would allow accurate control of the temperature within the columns, but the temperature profiles within the columns could not be characterized using the previously available small-internal-diameter scaffolded columns. In the current study the internal diameter of the scaffolded columns was increased up to square conduits of 4×4 mm. Prior to the formation of the stationary phase the heating efficiency in the empty scaffolded conduits was addressed. The performance of stationary phases created in the large scaffolds was investigated using the kinetic performance approach and the results were compared to those of the previous studies. Finally, scaffolded columns were tested under ultra-high-pressure LC conditions, where good temperature control is essential.
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http://dx.doi.org/10.1016/j.chroma.2015.05.003DOI Listing
July 2015

Characterization of hydroxypropylmethylcellulose (HPMC) using comprehensive two-dimensional liquid chromatography.

J Chromatogr A 2011 Aug 6;1218(34):5787-93. Epub 2011 May 6.

Van't Hoff Institute for Molecular Sciences, Faculty of Science, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands.

Various hydroxyl-propylmethylcellulose (HPMC) polymers were characterized according to size and compositional distributions (percentage of methoxyl and hydroxyl-propoxyl substitution) by means of comprehensive two-dimensional liquid chromatography (LC×LC) using reversed-phase (RP) liquid chromatography in the first dimension and aqueous size-exclusion chromatography (aq-SEC) in the second dimension. RP separation was carried out in gradient-elution mode applying 0.05% TFA in water and 1-propanol, while 0.05% TFA in water was used as mobile phase in aqueous SEC. A two-position ten-port switching valve equipped with two storage loops was used to realize LC×LC. Detection of HPMC was accomplished by charged-aerosol detection (CAD). Data processing to visualize chromatograms was carried out using Matlab software. The significant influence of the LC×LC temperature on (the retention of) HPMC was studied using a column oven which allowed accurate temperature control. Due to the phenomenon of thermal gelation, which is a result of methyl and hydroxypropyl substitution of anhydroglucose units from the cellulose backbone, we were able to obtain additional, specific information on compositional characteristics of various HPMC samples. As the retention behaviour of gelated and non-gelated polymer proved to be different, the fraction of the polymer that is gelated in the chromatographic column could be monitored at different temperatures. Moreover, the temperature at which half of the polymer is gelated could be correlated with the cloud-point temperature. As a result, differences in inherent cloud points of modified cellulose can be used as a further distinguishing property in "temperature-responsive" LC×LC.
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http://dx.doi.org/10.1016/j.chroma.2011.04.076DOI Listing
August 2011
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