Publications by authors named "Shiwen Wang"

151 Publications

Lactoferrin may inhibit the development of cancer via its immunostimulatory and immunomodulatory activities (Review).

Int J Oncol 2021 Nov 17;59(5). Epub 2021 Sep 17.

The Key Laboratory of Carcinogenesis and Cancer Invasion of The Chinese Ministry of Education, The Third Affiliated Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.

Lactoferrin (Lf) is secreted by ectodermal tissue and has a structure similar to that of transferrin. Although Lf seems to be multifunctional, its main function is related to the natural defense system of mammals. The present review aims to highlight the major actions of Lf, including the regulation of cell growth, the inhibition of toxic compound formation, the removal of harmful free radicals and its important role in immune response regulation. Moreover, Lf has antibacterial, antiviral, antioxidant, anticancer and anti‑inflammatory activities. In addition, the use of Lf for functionalization of drug nanocarriers, with emphasis on tumor‑targeted drug delivery, is illustrated. Such effects serve as an important theoretical basis for its future development and application. In neurodegenerative diseases and the brains of elderly people, Lf expression is markedly upregulated. Lf may exert an anti‑inflammatory effect by inhibiting the formation of hydroxyl free radicals. Through its antioxidant properties, Lf can prevent DNA damage, thereby preventing tumor formation in the central nervous system. In addition, Lf specifically activates the p53 tumor suppressor gene.
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http://dx.doi.org/10.3892/ijo.2021.5265DOI Listing
November 2021

Editorial: Lipid Metabolism in Development and Environmental Stress Tolerance for Engineering Agronomic Traits.

Front Plant Sci 2021 24;12:739786. Epub 2021 Aug 24.

Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Toruń, Toruń, Poland.

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http://dx.doi.org/10.3389/fpls.2021.739786DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421538PMC
August 2021

High-density dispersion of CuN sites for HO activation toward enhanced Photo-Fenton performance in antibiotic contaminant degradation.

J Hazard Mater 2021 Aug 28;423(Pt A):127039. Epub 2021 Aug 28.

College of Urban Construction, Nanjing Tech University, Nanjing 211816, China. Electronic address:

In this study, a copper-based catalyst (CuCN) with CuN active sites highly dispersed in a porous carbon nitride matrix was synthesized and applied to a heterogeneous photo-assisted Photo-Fenton (PF) system to degrade tetracycline (TET). The results showed that the CuCN/PF system degraded up to 93.6% of TET within 60 min for ultrapure water matrix under the best experimental conditions, and more than 70% of TET for both river and lake water matrix. Toxicological tests suggested that the environmental risk caused by TET can be effectively inhibited by the CuCN/PF system. The good visible-light response and charge transport abilities of CuCN catalyst were identified in photoelectrochemical experiments. Free radical scavenging experiments and electron paramagnetic resonance (EPR) spectroscopy indicated that the active species in the degradation process were·OH, h,·O and O. Density functional theory (DFT) calculations revealed the positive effect of CuN sites in CuCN on the formation of hydroxyl radicals by activating HO. This work will provide a new insight for the development of high-efficiency heterogeneous catalysts in wastewater environmental remediation.
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http://dx.doi.org/10.1016/j.jhazmat.2021.127039DOI Listing
August 2021

Chemiluminescence Immunoassay Based Serological Immunoassays for Detection of SARS-CoV-2 Neutralizing Antibodies in COVID-19 Convalescent Patients and Vaccinated Population.

Viruses 2021 07 30;13(8). Epub 2021 Jul 30.

State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

The development of rapid serological detection methods re urgently needed for determination of neutralizing antibodies in sera. In this study, four rapid methods (ACE2-RBD inhibition assay, S1-IgG detection, RBD-IgG detection, and N-IgG detection) were established and evaluated based on chemiluminescence technology. For the first time, a broadly neutralizing antibody with high affinity was used as a standard for the quantitative detection of SARS-CoV-2 specific neutralizing antibodies in human sera. Sera from COVID-19 convalescent patients (N = 119), vaccinated donors (N = 86), and healthy donors (N = 299) confirmed by microneutralization test (MNT) were used to evaluate the above methods. The result showed that the ACE2-RBD inhibition assay calculated with either ACE2-RBD binding inhibition percentage rate or ACE2-RBD inhibiting antibody concentration were strongly correlated with MNT (r ≥ 0.78, < 0.0001) and also highly consistent with MNT (Kappa Value ≥ 0.94, < 0.01). There was also a strong correlation between the two evaluation indices (r ≥ 0.99, < 0.0001). Meanwhile, S1-IgG and RBD-IgG quantitative detection were also significantly correlated with MNT (r ≥ 0.73, < 0.0001), and both methods were highly correlated with each other (r ≥ 0.95, < 0.0001). However, the concentration of N-IgG antibodies showed a lower correlation with the MNT results (r < 0.49, < 0.0001). The diagnostic assays presented here could be used for the evaluation of SARS-CoV-2 vaccine immunization effect and serological diagnosis of COVID-19 patients, and could also have guiding significance for establishing other rapid serological methods to surrogate neutralization tests for SARS-CoV-2.
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http://dx.doi.org/10.3390/v13081508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402865PMC
July 2021

Antibody Cocktail Exhibits Broad Neutralization Activity Against SARS-CoV-2 and SARS-CoV-2 Variants.

Virol Sin 2021 Jul 5. Epub 2021 Jul 5.

State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has precipitated multiple variants resistant to therapeutic antibodies. In this study, 12 high-affinity antibodies were generated from convalescent donors in early outbreaks using immune antibody phage display libraries. Of them, two RBD-binding antibodies (F61 and H121) showed high-affinity neutralization against SARS-CoV-2, whereas three S2-target antibodies failed to neutralize SARS-CoV-2. Following structure analysis, F61 identified a linear epitope located in residues G446-S494, which overlapped with angiotensin-converting enzyme 2 (ACE2) binding sites, while H121 recognized a conformational epitope located on the side face of RBD, outside from ACE2 binding domain. Hence the cocktail of the two antibodies achieved better performance of neutralization to SARS-CoV-2. Importantly, these two antibodies also showed efficient neutralizing activities to the variants including B.1.1.7 and B.1.351, and reacted with mutations of N501Y, E484K, and L452R, indicated that it may also neutralize the recent India endemic strain B.1.617. The unchanged binding activity of F61 and H121 to RBD with multiple mutations revealed a broad neutralizing activity against variants, which mitigated the risk of viral escape. Our findings revealed the therapeutic basis of cocktail antibodies against constantly emerging SARS-CoV-2 variants and provided promising candidate antibodies to clinical treatment of COVID-19 patients infected with broad SARS-CoV-2 variants.
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http://dx.doi.org/10.1007/s12250-021-00409-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255729PMC
July 2021

Fangchinoline Inhibits Human Esophageal Cancer by Transactivating ATF4 to Trigger Both Noxa-Dependent Intrinsic and DR5-Dependent Extrinsic Apoptosis.

Front Oncol 2021 14;11:666549. Epub 2021 Jun 14.

Cancer Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Esophageal squamous cell carcinoma (ESCC) is a recalcitrant cancer. The Chinese herbal monomer fangchinoline (FCL) has been reported to have anti-tumor activity in several human cancer cell types. However, the therapeutic efficacy and underlying mechanism on ESCC remain to be elucidated. In the present study, for the first time, we demonstrated that FCL significantly suppressed the growth of ESCC both and . Mechanistic studies revealed that FCL-induced G1 phase cell-cycle arrest in ESCC which is dependent on p21 and p27. Moreover, we found that FCL coordinatively triggered Noxa-dependent intrinsic apoptosis and DR5-dependent extrinsic apoptosis by transactivating ATF4, which is a novel mechanism. Our findings elucidated the tumor-suppressive efficacy and mechanisms of FCL and demonstrated FCL is a potential anti-ESCC agent.
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http://dx.doi.org/10.3389/fonc.2021.666549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8236818PMC
June 2021

High-efficiency removal of tetracycline by carbon-bridge-doped g-CN/FeO magnetic heterogeneous catalyst through photo-Fenton process.

J Hazard Mater 2021 09 7;418:126333. Epub 2021 Jun 7.

Jiangsu Baichuan High-Tech New Materials Co., Ltd, Nanjing, China.

Carbon-bridge-modified malonamide (MLD)/g-CN (CN) was prepared by copolymerization of MLD with urea and melamine and loaded with FeO for the high-efficiency removal of tetracycline (TC) in water under photo-Fenton. The prepared catalysts were characterized by SEM, TEM, N adsorption-desorption analysis, XPS, XRD, and FTIR, which proved that the modification method successfully introduced the C bridge into the carbon nitride molecular system and increased the structural defects of the catalyst. The Carbon-bridge-modified MLD/CN/FeO also had good visible-light response and charge-separation and transport abilities in the photoelectrochemical test. Degradation results showed that the photo-Fenton degradation of TC reached 95.8%, and the mineralization rate was 55.7% within 80 min at 80 mM HO dosage, 0.5 g/L catalyst dosage, and near-neutral pH by 0.8MLD/CN/FeO. Moreover, the oxidation products and mineralization pathways of TC were explored by LC-MS. Toxicity analysis indicated low environmental threat of the intermediates in TC mineralization. EPR analysis and HO decomposition efficiency analyses showed an improvement in the HO decomposition performance of 0.8MLD/CN/FeO. This work could provide a valuable insight for the application of heterogeneous photo-Fenton technology in wastewater treatment.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126333DOI Listing
September 2021

Detection of Cadmium Toxicity in Plant.

Methods Mol Biol 2021 ;2326:267-271

Henan Collaborative Innovation Center of Modern Biological Breeding, Henan Institute of Science and Technology, Xinxiang, Henan, China.

Cadmium (Cd) is widespread in the soil, water, and atmosphere, so Cd toxicity to human can happen by breathing in air, drinking water, and eating food from plant grown in Cd-contaminated soil. Cd pollution draws a lot of attention from the scientific community and also regulatory agents and is researched widely by using both plant and animal system. In this protocol, the detection of cadmium (Cd) is described in soil and mature maize (Zea mays) plant with the atomic absorption spectrometer. The Cd uptake, translocation factor, and Cd health risk index are also introduced. The protocol can be modified slightly to measure Cd in different types of plants.
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http://dx.doi.org/10.1007/978-1-0716-1514-0_19DOI Listing
August 2021

Camptothecin Inhibits Neddylation to Activate the Protective Autophagy Through NF-B/AMPK/mTOR/ULK1 Axis in Human Esophageal Cancer Cells.

Front Oncol 2021 8;11:671180. Epub 2021 Apr 8.

Cancer Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

The neddylation pathway is overactivated in esophageal cancer. Our previous studies indicated that inactivation of neddylation by the NAE inhibitor induced apoptosis and autophagy in cancer cells. Camptothecin (CPT), a well-known anticancer agent, could induce apoptosis and autophagy in cancer cells. However, whether CPT could affect the neddylation pathway and the molecular mechanisms of CPT-induced autophagy in esophageal cancer remains elusive. We found that CPT induced apoptosis and autophagy in esophageal cancer. Mechanistically, CPT inhibited the activity of neddylation and induced the accumulation of p-IkBa to block NF-κB pathway. Furthermore, CPT induced the generation of ROS to modulate the AMPK/mTOR/ULK1 axis to finally promote protective autophagy. In our study, we elucidate a novel mechanism of the NF-B/AMPK/mTOR/ULK1 pathway in CPT-induced protective autophagy in esophageal cancer cells, which provides a sound rationale for combinational anti-ESCC therapy with CPT and inhibition AMPK/ULK1 pathway.
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http://dx.doi.org/10.3389/fonc.2021.671180DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8061413PMC
April 2021

Epidemiological Characteristics of Severe Fever with Thrombocytopenia Syndrome from 2010 to 2019 in Mainland China.

Int J Environ Res Public Health 2021 03 17;18(6). Epub 2021 Mar 17.

NHC Key Laboratory for Medical Viruses and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease and that is a severe threat to public health considering its high fatality and person-to-person transmission. In order to obtain an updated and deep understanding of the epidemiological characteristics of SFTS in mainland China, we used Pearson's chi-squared test to compare the fatality rate and demographic characteristics in different groups. Data were analyzed in R3.6.1 (R Development Core Team 2018), while the visualization was performed in ArcGIS 10 (ESRI, Redlands, CA, USA), and the statistical significance was set at < 0.05. A total of 13,824 SFTS cases involving 8899 lab-confirmed cases and 4925 probable cases were reported and included in the epidemiological analysis. Our study found that the number of SFTS cases showed an increasing trend with a small decrease in the past three years. The laboratory-confirmed rate was about 64.4%, which varied between different years and areas. Although most cases (99.3%) were distributed in 7 provinces (Henan, Shandong, Anhui, Hubei, Liaoning, Zhejiang, and Jiangsu), the regional distribution of SFTS gradually expanded from 5 provinces in 2010 to 25 provinces by 2019, especially at the town level. The SFTS cases were mainly sporadic. A total of 96.5% occurred from April to October, and 93.3% of cases were concentrated in middle-aged and elderly people (40-84 years old). Farmers were the main high-risk population. Female cases were slightly more than male cases; however, there were differences between different provinces. The mortality rate showed an increasing trend with age. Overall, the SFTS cases were mainly middle-aged and elderly farmers that sporadically distributed throughout seven provinces with a spatially expanding trend. The laboratory-confirmed rate varied in different years and provinces, which implied that the diagnosis and report criteria for SFTS should be further updated and unified in order to get a better understanding of its epidemiological characteristics and provide scientific data for SFTS control.
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http://dx.doi.org/10.3390/ijerph18063092DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002760PMC
March 2021

Abnormal upregulation of cardiovascular disease biomarker PLA2G7 induced by proinflammatory macrophages in COVID-19 patients.

Sci Rep 2021 03 24;11(1):6811. Epub 2021 Mar 24.

NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.

High rate of cardiovascular disease (CVD) has been reported among patients with coronavirus disease 2019 (COVID-19). Importantly, CVD, as one of the comorbidities, could also increase the risks of the severity of COVID-19. Here we identified phospholipase A2 group VII (PLA2G7), a well-studied CVD biomarker, as a hub gene in COVID-19 though an integrated hypothesis-free genomic analysis on nasal swabs (n = 486) from patients with COVID-19. PLA2G7 was further found to be predominantly expressed by proinflammatory macrophages in lungs emerging with progression of COVID-19. In the validation stage, RNA level of PLA2G7 was identified in nasal swabs from both COVID-19 and pneumonia patients, other than health individuals. The positive rate of PLA2G7 were correlated with not only viral loads but also severity of pneumonia in non-COVID-19 patients. Serum protein levels of PLA2G7 were found to be elevated and beyond the normal limit in COVID-19 patients, especially among those re-positive patients. We identified and validated PLA2G7, a biomarker for CVD, was abnormally enhanced in COVID-19 at both nucleotide and protein aspects. These findings provided indications into the prevalence of cardiovascular involvements seen in patients with COVID-19. PLA2G7 could be a potential prognostic and therapeutic target in COVID-19.
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http://dx.doi.org/10.1038/s41598-021-85848-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990942PMC
March 2021

Overexpressed NEDD8 as a potential therapeutic target in esophageal squamous cell carcinoma.

Cancer Biol Med 2021 Mar 18. Epub 2021 Mar 18.

Department of Laboratory Medicine, Huadong Hospital Affiliated to Fudan University, Shanghai 200040, China.

Objective: The hyperactivated neddylation pathway plays an important role in tumorigenesis and is emerging as a promising anticancer target. We aimed to study whether NEDD8 (neural precursor cell expressed, developmentally down-regulated 8) might serve as a therapeutic target in esophageal squamous cell carcinoma (ESCC).

Methods: The clinical relevance of NEDD8 expression was evaluated by using The Cancer Genome Atlas (TCGA) database and tissue arrays. NEDD8-knockdown ESCC cells generated with the CRISPR/Cas9 system were used to explore the anticancer effects and mechanisms. Quantitative proteomic analysis was used to examine the variations in NEDD8 knockdown-induced biological pathways. The cell cycle and apoptosis were assessed with fluorescence activated cell sorting. A subcutaneous-transplantation mouse tumor model was established to investigate the anticancer potential of NEDD8 silencing .

Results: NEDD8 was upregulated at both the mRNA and protein expression levels in ESCC, and NEDD8 overexpression was associated with poorer overall patient survival (mRNA level: = 0.028, protein level: = 0.026, log-rank test). Downregulation of NEDD8 significantly suppressed tumor growth both and . Quantitative proteomic analysis revealed that downregulation of NEDD8 induced cell cycle arrest, DNA damage, and apoptosis in ESCC cells. Mechanistic studies demonstrated that NEDD8 knockdown led to the accumulation of cullin-RING E3 ubiquitin ligases (CRLs) substrates through inactivation of CRLs, thus suppressing the malignant phenotype by inducing cell cycle arrest and apoptosis in ESCC. Rescue experiments demonstrated that the induction of apoptosis after NEDD8 silencing was attenuated by DR5 knockdown.

Conclusions: Our study elucidated the anti-ESCC effects and underlying mechanisms of NEDD8 knockdown, and validated NEDD8 as a potential target for ESCC therapy.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2020.0484DOI Listing
March 2021

Cullin3-TNFAIP1 E3 Ligase Controls Inflammatory Response in Hepatocellular Carcinoma Cells via Ubiquitination of RhoB.

Front Cell Dev Biol 2021 21;9:617134. Epub 2021 Jan 21.

Department of Laboratory Medicine, Huadong Hospital Affiliated to Fudan University, Shanghai, China.

Rho family GTPase RhoB is the critical signaling component controlling the inflammatory response elicited by pro-inflammatory cytokines. However, the underlying mechanisms of RhoB degradation in inflammatory response remain unclear. In this study, for the first time, we identified that TNFAIP1, an adaptor protein of Cullin3 E3 ubiquitin ligases, coordinated with Cullin3 to mediate RhoB degradation through ubiquitin proteasome system. In addition, we demonstrated that downregulation of TNFAIP1 induced the expression of pro-inflammatory cytokines IL-6 and IL-8 in TNFα-stimulated hepatocellular carcinoma cells through the activation of p38/JNK MAPK pathway via blocking RhoB degradation. Our findings revealed a novel mechanism of RhoB degradation and provided a potential strategy for anti-inflammatory intervention of tumors by targeting TNFAIP1-RhoB axis.
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http://dx.doi.org/10.3389/fcell.2021.617134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859282PMC
January 2021

Late-onset Hearing Loss From Congenital Cytomegalovirus Infection After Newborn Period in a Highly Immune Population in China.

Pediatr Infect Dis J 2021 01;40(1):70-73

From the Centers for Disease Control and Prevention, Atlanta, Georgia.

After following 141 children with likely asymptomatic congenital cytomegalovirus infection in a highly immune population in China, four children (2.8%) were found to have late-onset hearing loss. No maternal or childhood factors, except higher saliva cytomegalovirus viral load at birth (P = 0.03), were associated with increased risk of developing a hearing loss.
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http://dx.doi.org/10.1097/INF.0000000000002922DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383185PMC
January 2021

The long noncoding RNA HCG18 participates in PM2.5-mediated vascular endothelial barrier dysfunction.

Aging (Albany NY) 2020 11 16;12(23):23960-23973. Epub 2020 Nov 16.

GMU-GIBH Joint School of Life Sciences, Center of Reproductive Medicine, Third Affiliated Hospital, Guangzhou Medical University, Guangzhou 510182, China.

Increased vascular endothelial permeability can disrupt vascular barrier function and further lead to multiple human diseases. Our previous reports indicated that particulate matter 2.5 (PM2.5) can enhance the permeability of vascular endothelial cells. However, the regulatory mechanism was not comprehensively demonstrated. Therefore, this work elucidated this mechanism by demonstrating that PM2.5 can increase the permeability of HUVECs by inhibiting the expression of Hickson compact group 18 (HCG18). Moreover, we demonstrated that lncRNA HCG18 functioned as a ceRNA for miR-21-5p and led to the derepression of its target SOX7, which could further transcriptionally activate the expression of VE-cadherin to regulate the permeability of HUVECs. In this study, we provide evidence that HCG18/miR-21-5p/SOX7/VE-cadherin signaling is involved in PM2.5-induced vascular endothelial barrier dysfunction.
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http://dx.doi.org/10.18632/aging.104073DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762519PMC
November 2020

The development of an antifouling interpenetrating polymer network hydrogel film for salivary glucose monitoring.

Nanoscale 2020 Nov;12(44):22787-22797

School of Materials Science and Engineering, Zhengzhou University, Zhengzhou 450001, P. R. China.

Owing to its rapid response and broad detection range, a phenylboronic acid (PBA)-functionalized hydrogel film-coated quartz crystal microbalance (QCM) sensor is used to non-invasively monitor salivary glucose in diabetic patients. However, nonspecific protein adsorption on the PBA-functionalized hydrogel film can cause dramatic loss of sensitivity and accuracy of the sensor. A traditional zwitterionic polymer surface with ultra-low protein fouling can hinder the interaction of PBA in the hydrogel matrix with glucose molecules owing to its steric hindrance, resulting in poor glucose sensitivity of the sensor. Herein, we developed a novel hydrogel film that enhanced the antifouling properties and sensitivity of the QCM sensor by infiltrating a glucose-sensitive monomer (i.e., PBA) into a zwitterionic polymer brush matrix to form an interpenetrating polymer network (IPN). The IPN hydrogel film could minimize the glucose sensitivity loss since the antifouling polymer distributed in its matrix. Moreover, a stable hydration layer was formed in this film that could prevent water from transporting out of the matrix, thus further improving its antifouling properties and glucose sensitivity. The experimental results confirmed that the IPN hydrogel film possessed excellent resistance to protein fouling by mucin from whole saliva with reductions in adsorption of nearly 88% and could also enhance the glucose sensitivity by nearly 2 fold, compared to the PBA-functionalized hydrogel film. Therefore, the IPN hydrogel film provides improved antifouling properties and sensitivity of the QCM sensor, which paves the way for non-invasive monitoring of low concentrations of glucose in saliva.
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http://dx.doi.org/10.1039/d0nr05854hDOI Listing
November 2020

Comprehensive evaluation of physiological traits under nitrogen stress and participation of linolenic acid in nitrogen-deficiency response in wheat seedlings.

BMC Plant Biol 2020 Nov 3;20(1):501. Epub 2020 Nov 3.

State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Background: Nitrogen (N) deficiency is a major constraint for plant production in many areas. Developing the new crop genotypes with high productivity under N deficiency is an important approach to maintain agricultural production. Therefore, understanding how plant response to N deficiency and the mechanism of N-deficiency tolerance are very important for sustainable development of modern crop production.

Results: In this study, the physiological responses and fatty acid composition were investigated in 24 wheat cultivars under N-deficient stress. Through Pearson's correlation analysis and principal component analysis, the responses of 24 wheat cultivars were evaluated. The results showed that the plant growth and carbohydrate metabolism were all differently affected by N deficiency in all tested wheat cultivars. The seedlings that had high shoot biomass also maintained high level of chlorophyll content under N deficiency. Moreover, the changes in fatty acid composition, especially the linolenic acid (18:3) and the double bond index (DBI), showed close positive correlations with the shoot dry weight and chlorophyll content alterations in response to N-deficient condition. These results indicated that beside the chlorophyll content, the linolenic acid content and DBI may also contribute to N-deficiency adaptation, thus could be considered as efficient indicators for evaluation of different response in wheat seedlings under N-deficient condition.

Conclusions: The alteration in fatty acid composition can potentially contribute to N-deficiency tolerance in plants, and the regulation of fatty acid compositions maybe an effective strategy for plants to adapt to N-deficient stress.
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http://dx.doi.org/10.1186/s12870-020-02717-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7607636PMC
November 2020

Development of Sarcophaga dux (diptera: Sarcophagidae) at constant temperatures and differential gene expression for age estimation of the pupae.

J Therm Biol 2020 Oct 17;93:102735. Epub 2020 Sep 17.

Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha, 410013, Hunan, China. Electronic address:

Sarcophaga dux (Diptera: Sarcophagidae) is a necrophagous flesh fly species with potential forensic value for estimating minimum postmortem interval (PMI). The basic developmental data and precise age estimates of the pupae are significant for PMI estimation in forensic investigations. In the present study, we investigated the development data of that species at seven constant temperatures varying from 16 °C to 34 °C, including body length changes of the larve, developmental duration and accumulated degree hours of the preadults. Several reference genes for relative quantification of the differentially expressed genes (DEGs) were firstly selected and evaluated in the pupae of different ages under different temperatures. The DEGs of the insects during the pupal period at different constant temperatures (34, 25 and 16 °C) were further analyzed for more precise age estimation. The results showed that the developmental durations of the preadults at 16, 19, 22, 25, 28, 31 and 34 °C were 1478.6 ± 18.3 h, 726.1 ± 15.8 h, 538.5 ± 0.9 h, 394.1 ± 9.5 h, 375.6 ± 10.8 h, 284.1 ± 7.3 h, and 252.5 ± 6.1 h, respectively. The developmental threshold temperature the flies was 12.27 ± 0.35 °C, and the thermal summation constant was 5341.71 ± 249.29° hours. The most reliable reference genes during the pupal period at different temperatures were found: GST1 and 18S rRNA for the 34 °C group, GST1 and RPL49 for 25 °C, and 18S rRNA and 28S rRNA for 16 °C. The four differential expression genes (Hsp60, A-alpha, ARP, and RPL8) have the potential to be used for more precise age estimation of pupal S. dux. This work provides important basic developmental data and a more precise age estimation method for pupal S. dux, and improves the value of this species for PMI estimation in forensic investigations.
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http://dx.doi.org/10.1016/j.jtherbio.2020.102735DOI Listing
October 2020

A highly sensitive quartz crystal microbalance sensor modified with antifouling microgels for saliva glucose monitoring.

Nanoscale 2020 Oct 16;12(37):19317-19324. Epub 2020 Sep 16.

School of Materials Science and Engineering, Zhengzhou University, Zhengzhou 450001, P. R. China.

Saliva glucose detection based on quartz crystal microbalance (QCM) technology has become an important research direction of non-invasive blood glucose monitoring. However, the performance of this label-free glucose sensor is heavily deteriorated by the large amount of protein contaminants in saliva. Here, we successfully achieved the direct detection of saliva glucose by endowing the microgels on the QCM chip with superior protein-resistive and glucose-sensitive properties. Specifically, the microgel networks provide plenty of boric acid binding sites to amplify the signals of targeted glucose. The amino acid layer wrapped around the microgel and crosslinking layer can effectively eliminate the impact of non-specific proteins in saliva. The designed QCM sensor has a good linearity in the glucose concentration range of 0-40 mg L in the pH range of 6.8-7.5, satisfying the physiological conditions of saliva glucose. Moreover, the sensor has excellent ability to tolerate proteins, enabling it to detect glucose in 50% human saliva. This result provides a new approach for non-invasive blood glucose monitoring based on QCM.
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http://dx.doi.org/10.1039/d0nr03193cDOI Listing
October 2020

Arabidopsis mgd mutants with reduced monogalactosyldiacylglycerol contents are hypersensitive to aluminium stress.

Ecotoxicol Environ Saf 2020 Oct 21;203:110999. Epub 2020 Jul 21.

State Key Laboratory of Crop Stress Biology in Arid Areas/College of Agronomy, Northwest A & F University, Yangling, Shaanxi, 712100, PR China. Electronic address:

Aluminium (Al) is a key element that plays a major role in inhibiting plant growth and productivity under acidic soils. While lipids may be involved in plant tolerance/sensitivity to Al, the role of monogalactosyldiacylglycerol (MGDG) in Al response remains unknown. In this study, Arabidopsis MGDG synthase (AtMGD) mutants (mgd1, mgd2 and mgd3) and wild-type (Col-0) plants were treated with AlCl; the effect of aluminium on root growth, aluminium distribution, plasma membrane integrity, lipid peroxidation, hydrogen peroxide content and membrane lipid compositions were analysed. Under Al stress, mgd mutants exhibited a more severe root growth inhibition, plasma membrane integrity damage and lipid peroxidation compared to Col-0. Al accumulation in root tips showed no difference between Col-0 and mutants under Al stress. Lipid analysis demonstrated that under Al treatment the MGDG content in all plants and MGDG/DGDG (digalactosyldiacylglycerol) remarkably reduced, especially in mutants impairing the stability and permeability of the plasma membrane. These results indicate that the Arabidopsis mgd mutants are hypersensitive to Al stress due to the reduction in MGDG content, and this is of great significance in the discovery of effective measures for plants to inhibit aluminium toxicity.
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http://dx.doi.org/10.1016/j.ecoenv.2020.110999DOI Listing
October 2020

3D porous graphene composite film embedded by Ni/NiO nanoparticles as freestanding electrodes for efficient energy storage devices.

Nanotechnology 2020 Nov;31(47):475704

State Laboratory of Surface and Interface, Zhengzhou University of Light Industry, Zhengzhou 450002, People's Republic of China.

A 3D porous graphene composite film containing Ni/NiO hybrid nanoparticles (Ni/NiO NPs) is prepared by combining electrophoresis deposition and thermal H annealing techniques. The Ni/NiO NPs with a mean diameter of 45 nm are uniformly embedded on both the exterior and interior surfaces of reduced graphene, forming a 3D porous reduced graphene oxide composite film (Ni/NiO rGO). The insertion of Ni/NiO NPs into rGO greatly improves the electric conductivity and charge storage capability of the resultant Ni/NiO rGO film. By directly using it as freestanding electrodes, the fabricated lithium-ion battery and supercapacitor respectively exhibited high capacities of 758 mAh [email protected] 0.2 A g and 430.8 F [email protected] A g, an increase of 82.3-fold and 20.2-fold compared to the pure rGO electrode-based counterparts under the same condition.
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http://dx.doi.org/10.1088/1361-6528/abae2dDOI Listing
November 2020

Chromosome-level de novo genome assembly of Sarcophaga peregrina provides insights into the evolutionary adaptation of flesh flies.

Mol Ecol Resour 2021 Jan 15;21(1):251-262. Epub 2020 Sep 15.

Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha, China.

Sarcophaga peregrina is considered to be of great ecological, medical and forensic significance, and has unusual biological characteristics such as an ovoviviparous reproductive pattern and adaptation to feed on carrion. The availability of a high-quality genome will help to further reveal the mechanisms underlying these charcateristics. Here we present a de novo-assembled genome at chromosome scale for S. peregrina. The final assembled genome was 560.31 Mb with contig N50 of 3.84 Mb. Hi-C scaffolding reliably anchored six pseudochromosomes, accounting for 97.76% of the assembled genome. Moreover, 45.70% of repeat elements were identified in the genome. A total of 14,476 protein-coding genes were functionally annotated, accounting for 92.14% of all predicted genes. Phylogenetic analysis indicated that S. peregrina and S. bullata diverged ~ 7.14 million years ago. Comparative genomic analysis revealed expanded and positively selected genes related to biological features that aid in clarifying its ovoviviparous reproduction and carrion-feeding adaptations, such as lipid metabolism, olfactory receptor activity, antioxidant enzymes, proteolysis and serine-type endopeptidase activity. Protein-coding genes associated with ovoviparity, such as yolk proteins, transferrin and acid sphingomyelinase, were identified. This study provides a valuable genomic resource for S. peregrina, and sheds insight into further revealing the underlying molecular mechanisms of adaptive evolution.
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http://dx.doi.org/10.1111/1755-0998.13246DOI Listing
January 2021

Single virus tracking of Ebola virus entry through lipid rafts in living host cells.

Biosaf Health 2020 Mar 2;2(1):25-31. Epub 2020 Jan 2.

Key Laboratory of Medical Virology and Viral Diseases, Ministry of Health of People's Republic of China, National Institute for Viral Disease Control and Prevention (IVDC), Chinese Center for Disease control and Prevention (China CDC), Beijing 102206, China.

Ebola virus (EBOV) is one of the most pathogenic viruses in humans which can cause a lethal hemorrhagic fever. Understanding the cellular entry mechanisms of EBOV can promote the development of new therapeutic strategies to control virus replication and spread. It has been known that EBOV virions bind to factors expressed at the host cell surface. Subsequently, the virions are internalized by a macropinocytosis-like process, followed by being trafficked through early and late endosomes. Recent researches indicate that the entry of EBOV into cells requires integrated and functional lipid rafts. Whilst lipid rafts have been hypothesized to play a role in virus entry, there is a current lack of supporting data. One major technical hurdle is the lack of effective approaches for observing viral entry. To provide evidence on the involvement of lipid rafts in the entry process of EBOV, we generated the fluorescently labeled Ebola virus like particles (VLPs), and utilized single-particle tracking (SPT) to visualize the entry of fluorescent Ebola VLPs in live cells and the interaction of Ebola VLPs with lipid rafts. In this study, we demonstrate the compartmentalization of Ebola VLPs in lipid rafts during entry process, and inform the essential function of lipid rafts for the entry of Ebola virus. As such, our study provides evidence to show that the raft integrity is critical for Ebola virus pathogenesis and that lipid rafts can serve as potential targets for the development of novel therapeutic strategies.
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http://dx.doi.org/10.1016/j.bsheal.2019.12.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347359PMC
March 2020

Genetic diversity and evolution of Hantaan virus in China and its neighbors.

PLoS Negl Trop Dis 2020 08 20;14(8):e0008090. Epub 2020 Aug 20.

Key Laboratory of Medical Virology and Viral Diseases, Ministry of Health of People's Republic of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

Background: Hantaan virus (HTNV; family Hantaviridae, order Bunyavirales) causes hemorrhagic fever with renal syndrome (HFRS), which has raised serious concerns in Eurasia, especially in China, Russia, and South Korea. Previous studies reported genetic diversity and phylogenetic features of HTNV in different parts of China, but the analyses from the holistic perspective are rare.

Methodology And Principal Findings: To better understand HTNV genetic diversity and gene evolution, we analyzed all available complete sequences derived from the small (S) and medium (M) segments with bioinformatic tools. Eleven phylogenetic groups were defined and showed geographic clustering; 42 significant amino acid variant sites were found, and 19 of them were located in immune epitopes; nine recombinant events and eight reassortments with highly divergent sequences were found and analyzed. We found that sequences from Guizhou showed high genetic divergence, contributing to multiple lineages of the phylogenetic tree and also to the recombination and reassortment events. Bayesian stochastic search variable selection analysis revealed that Heilongjiang, Shaanxi, and Guizhou played important roles in HTNV evolution and migration; the virus may originate from Zhejiang Province in the eastern part of China; and the virus population size expanded from the 1980s to 1990s.

Conclusions/significance: These findings revealed the original and evolutionary features of HTNV, which will help to illustrate hantavirus epidemic trends, thus aiding in disease control and prevention.
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http://dx.doi.org/10.1371/journal.pntd.0008090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7462299PMC
August 2020

Evaluation of Reference Genes and Age Estimation of Forensically Useful Aldrichina grahami (Diptera: Calliphoridae) During Intrapuparial Period.

J Med Entomol 2021 01;58(1):47-55

Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha, Hunan, China.

The minimum postmortem interval (PMImin) could be evaluated from the developmental stage of forensically important insects colonize a corpse, such as blow flies (Diptera: Calliphoridae). Unlike larvae, the developmental stage of which is well established according to their morphology, estimating the age of pupae is proven to be challenging. Recently, several studies reported the regulation of special genes during the development of blow fly pupae. However, gene regulation in Aldrichina grahami during the intrapuparial period remains to be studied. Therefore, we set out to investigate the mRNA levels of heat shock protein 23 (Hsp23), heat shock protein 24 (Hsp24), and 1_16 during the metamorphosis of A. grahami pupae. First, we examined seven candidate reference genes (ribosomal protein 49 (RP49), 18S ribosomal RNA (18S rRNA), 28S ribosomal RNA (28S rRNA), beta-tubulin at 56D (β-tubulin), Ribosomal protein L23 (RPL23), glutathione S-transferase (GST1), and Actin. Three widely used algorithms (NormFinder, BestKeeper, and geNorm) were applied to evaluate the mRNA levels of reference gene candidates in puparium at three stable temperatures (15, 22, and 27°C). Next, mRNA expression of Hsp23, Hsp24, and 1_16 during A. grahami metamorphosis was examined. We demonstrated that mRNA expression levels of Hsp23, Hsp24, and 1_16 showed time-specific regulation. In summary, our study identified three gene markers for the intrapuparial period of A. grahami and might provide a potential application in PMImin estimation.
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http://dx.doi.org/10.1093/jme/tjaa144DOI Listing
January 2021

NEDD8-conjugating enzyme UBC12 as a novel therapeutic target in esophageal squamous cell carcinoma.

Signal Transduct Target Ther 2020 07 10;5(1):123. Epub 2020 Jul 10.

Department of Laboratory Medicine, Huadong Hospital Affiliated to Fudan University, Shanghai, 200040, China.

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http://dx.doi.org/10.1038/s41392-020-00226-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7351728PMC
July 2020

Ultrasensitive Poly(boric acid) Hydrogel-Coated Quartz Crystal Microbalance Sensor by Using UV Pressing-Assisted Polymerization for Saliva Glucose Monitoring.

ACS Appl Mater Interfaces 2020 Jul 20;12(30):34190-34197. Epub 2020 Jul 20.

Division of Nanophotonics, CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, P. R. China.

Quartz crystal microbalance (QCM) has attracted extensive attention in the field of biological analysis and detection because of its high sensitivity, fast response, real-time measurement, good operability, and low-cost production. However, to detect the trace amounts of small molecules, such as low-concentration saliva glucose under physiological conditions, is still a major challenge. Herein, the surface of a QCM chip was coated with a poly(boric acid)-based hydrogel using UV pressing-assisted polymerization to obtain a simple device for glucose detection. The designed QCM sensor shows a record-low detection limit of glucose (3 mg/L at pH 7.5), which is ∼30 times lower than that of sensors fabricated by conventional surface initiation-spin coating. The outperformance of the poly(boric acid) hydrogel-coated QCM sensor is probably due to the uniform and compact microstructure, as well as the presence of sufficient glucose-binding sites resulting from the hydrogel coating generated by UV pressing-assisted polymerization. This method provides an important solution to detect the trace amounts of small organic molecules or ions and has the potential to push forward the practical applications of QCM sensors.
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http://dx.doi.org/10.1021/acsami.0c08229DOI Listing
July 2020

Comparative analysis of mitochondrial genomes among the subfamily Sarcophaginae (Diptera: Sarcophagidae) and phylogenetic implications.

Int J Biol Macromol 2020 Oct 8;161:214-222. Epub 2020 Jun 8.

Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha, Hunan, China. Electronic address:

The subfamily Sarcophaginae is extremely diverse in morphology, habit and geographical distribution, and usually considered to be of significant ecological, medical, and forensic significance. In the present study, 18 mitochondrial genomes (mitogenomes) of sarcophagid flies were first obtained. The rearrangement and orientation of genes were identical with that of ancestral insects. The degrees of compositional heterogeneity in the datasets were extremely low. Furthermore, 13 protein-coding genes were evolving under purifying selection. The phylogenic relationship of the genus-group taxa Boettcheria + (Sarcophaga + (Peckia + (Ravinia + Oxysarcodexia))) was strongly supported. Four subgenera were recovered as monophyletic (Liopygia, Liosarcophaga, Pierretia, Heteronychia) in addition to Parasarcophaga as polyphyletic. The sister-relationships between S. dux and S. aegyptiaca, S. pingi and S. kawayuensis were recovered, respectively. Moreover, the molecular phylogenetic relationships among the subgenera Helicophagella, Kozlovea, Kramerea, Pandelleisca, Phallocheira, Pseudothyrsocnema, Sinonipponia and Seniorwhitea were rarely put forward prior to this study. This study provides insight into the population genetics, molecular biology, and phylogeny for the subfamily Sarcophaginae, especially for the subgeneric classification of Sarcophaga. However, compared with the enormous species diversity of flesh flies, the available mitogenomes are still limited for recovering the phylogeny of Sarcophaginae.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.06.043DOI Listing
October 2020

Exogenous melatonin alleviates PEG-induced short-term water deficiency in maize by increasing hydraulic conductance.

BMC Plant Biol 2020 May 14;20(1):218. Epub 2020 May 14.

State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Background: Water deficiency is likely to become more frequent and intense as a result of global climate change, which may severely impact agricultural production in the world. The positive effects of melatonin (MEL) on alleviation drought or osmotic stress-induced water deficiency in plants has been well reported. However, the underlying mechanism of MEL on the detailed process of plant water uptake and transport under water deficiency condition remains largely unknown.

Results: Application of 1 μM MEL led to enhanced tolerance to water deficiency stress in maize seedlings, as evidenced by maintaining the higher photosynthetic parameters, leaf water status and plant transpiration rate. The relatively higher whole-plant hydraulic conductance (K) and root hydraulic conductance (Lp) in MEL-treated seedlings suggest that exogenous MEL alleviated water deficiency stress by promoting root water absorption. HgCl (aquaporin inhibitor) treatment inhibit the transpiration rate in MEL-treated plants greater than those of MEL-untreated; after recovery by dithiothreitol (DTT, anti-inhibitor), the transpiration rate in MEL-treated plants increased much higher than those of untreated plants. Moreover, under water deficiency, the transcription level of aquaporin genes was up-regulated by MEL application, and the HO was less accumulated in MEL-treated root.

Conclusions: Exogenous MEL promoted aquaporin activity, which contributed to the maintaining of Lp and K under short-term water deficiency. The increased water uptake and transport lead to improved water status and thus increased tolerance to PEG-induced short-term water deficiency in maize seedlings.
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http://dx.doi.org/10.1186/s12870-020-02432-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227339PMC
May 2020

Combined application of silicon and nitric oxide jointly alleviated cadmium accumulation and toxicity in maize.

J Hazard Mater 2020 Aug 13;395:122679. Epub 2020 Apr 13.

Henan Key Laboratory for Molecular Ecology and Germplasm Innovation of Cotton and Wheat/Henan Collaborative Innovation Center of Modern Biological Breeding, Henan Institute of Science and Technology, Xinxiang, Henan, 453003, China. Electronic address:

Cadmium (Cd) contamination is a serious threat to plants and humans. Application of silicon (Si) or nitric oxide (NO) could alleviate Cd accumulation and toxicity in plants, but whether they have joint effects on alleviating of Cd accumulation and toxicity are not known. Therefore, the combined effect of Si and NO application on maize growth, Cd uptake, Cd transports and Cd accumulation were investigated in a pot experiment. Here, we reported that Cd stress decreased growth, caused Cd accumulation in plants. The combined application of Si and NO triggered a significant response in maize, increasing plant growth and reducing Cd uptake, accumulation, translocation and bioaccumulation factors under Cd stress. The grain Cd concentration was decreased by 66 % in the Si and NO combined treatment than Cd treatment. Moreover, the combined application of Si and NO reduced Cd health risk index in maize more effectively than either treatment alone. This study provided new evidence that Si and NO have a strong joint effect on alleviating the adverse effects of Cd toxicity by decreasing Cd uptake and accumulation. We advocate for supplement of Cd-contaminated soil with Si fertilizers and treatment of crops with NO as a practical approach to alleviating Cd toxicity.
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http://dx.doi.org/10.1016/j.jhazmat.2020.122679DOI Listing
August 2020
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