Publications by authors named "Shiwei Li"

67 Publications

Natural course of myopic traction maculopathy and factors influencing progression and visual acuity.

BMC Ophthalmol 2021 Sep 25;21(1):347. Epub 2021 Sep 25.

Department of Ophthalmology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, No. 600 Yishan Rd, 200233, Shanghai, China.

Background: To describe the natural course of myopic traction maculopathy (MTM) and determine predictive factors for its progression and visual prognosis.

Methods: This retrospective observational study included 113 MTM patients (113 eyes). Best-corrected visual acuity (BCVA) measurements and optical coherence tomography findings were recorded.

Results: Over a mean follow-up of 38.2 ± 11.1 months, 49 of 113 eyes (43.4 %) progressed. The progression rate of outer schisis prominently located in the fovea or posterior staphyloma was significantly higher than that of outer schisis prominently located in paravascular areas (P = 0.0011). MTM with partial posterior vitreous detachment during the follow-up progressed more rapidly than MTM without (P = 0.0447). Patients with older age (> 65 years), without domed-shaped macula and with defects in the ellipsoid zone (EZ) had worse BCVA at the last visit (P = 0.0416, P = 0.0494and P = 0.0130). Multiple linear regression analysis showed that BCVA and defects in the EZ at baseline were significantly associated with the final BCVA (P < 0.0001 and P = < 0.0001, respectively).

Conclusions: MTM has a high possibility for progression. Outer schisis located predominantly in the fovea or posterior staphyloma or with partial posterior vitreous detachment exhibits rapid progression. The integrity of the EZ is related to visual prognosis.
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http://dx.doi.org/10.1186/s12886-021-02087-yDOI Listing
September 2021

LncRNA SOX2-OT regulates miR-192-5p/RAB2A axis and ERK pathway to promote glioblastoma cell growth.

Cell Cycle 2021 Sep 1:1-11. Epub 2021 Sep 1.

Department of Neurosurgery, Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai, China.

Glioblastoma (GBM) is the most frequent tumor in the central nervous system. Long non-coding RNAs (lncRNAs) have been widely accepted as essential participators in cancer progression. Nonetheless, the specific role and mechanism of lncRNA SRY-box transcription factor 2 overlapping transcript (SOX2-OT) in GBM have not been studied. We evaluated expression levels of SOX2-OT, miR-192-5p and Ras-related protein Rab-2A (RAB2A) in GBM cells via qRT-PCR. To investigate the roles of SOX2-OT in GBM cells, CCK-8, JC-1, EdU, and western blot assays were performed. The connection among SOX2-OT, miR-192-5p and RAB2A in GBM cells was explored through pull down, luciferase reporter, and RIP assays. Western blot and qRT-PCR were employed to analyze the activity of extracellular-signal-regulated kinase (ERK) signaling pathway. SOX2-OT expression was higher in GBM cell lines than in normal cells. SOX2-OT knockdown repressed proliferation and promoted apoptosis of GBM cells. Mechanism assays revealed that SOX2-OT could sponge miR-192-5p. Moreover, RAB2A was certified to be the target gene of miR-192-5p. Overexpression of RAB2A reversed the repressive function of SOX2-OT knockdown on GBM cell growth. Furthermore, SOX2-OT activated ERK signaling pathway in GBM cells. SOX2-OT regulated miR-192-5p/RAB2A axis and ERK pathway to promote GBM cell growth.
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http://dx.doi.org/10.1080/15384101.2021.1965722DOI Listing
September 2021

Naloxone Protects against Lipopolysaccharide-Induced Neuroinflammation and Microglial Activation via Inhibiting ATP-Sensitive Potassium Channel.

Comput Math Methods Med 2021 29;2021:7731528. Epub 2021 Jul 29.

Department of Neurosurgery, Haimen People's Hospital, Nantong 226100, China.

Aim: The aim of this study was to evaluate the anti-inflammatory effects and underlying mechanism of naloxone on lipopolysaccharide- (LPS-) induced neuronal inflammation and microglial activation.

Methods: LPS-treated microglial BV-2 cells and mice were used to investigate the anti-inflammatory effects of naloxone.

Results: The results showed that naloxone dose-dependently promoted cell proliferation in LPS-induced BV-2 cells, downregulated the expression of proinflammatory cytokines (TNF-, IL-1, and IL-6) and proinflammatory enzymes iNOS and COX-2 as well as the expression of free radical molecule NO, and reduced the expression of Iba-1-positive microglia in LPS-stimulated BV-2 cells and mouse brain. Moreover, naloxone improved LPS-induced behavior degeneration in mice. Mechanically, naloxone inhibited LPS-induced activation in the ATP-sensitive potassium (KATP) channel. However, the presence of glibenclamide (Glib), an antagonist of KATP channel, ameliorated the suppressive effects of naloxone on inflammation and microglial activation.

Conclusion: Naloxone prevented LPS-induced neuroinflammation and microglial activation partially through the KATP channel. These findings might highlight the potential of naloxone in neuroinflammation therapy.
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http://dx.doi.org/10.1155/2021/7731528DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349287PMC
July 2021

A new inflammatory parameter can predict delayed intracranial hemorrhage following ventriculoperitoneal shunt.

Sci Rep 2021 Jul 2;11(1):13763. Epub 2021 Jul 2.

Neurosurgery Department of Ningbo Medical Center Lihuili Hospital, Zhejiang, China.

Delayed intracerebral hemorrhage (DICH) secondary to ventriculoperitoneal (VP) shunt is considered to be a potentially severe event. This study aimed to investigate the association between a ratio of postoperative neutrophil-to-lymphocyte ratio to preoperative neutrophil-to-lymphocyte ratio (NLRR) and DICH secondary to VP shunt. We performed a retrospective review of patients who underwent VP shunt between January 2016 and June 2020. Multivariable logistic regression analysis was used to assess the association of DICH and NLRR. Then patients were divided into two groups according to the optimal cut-off point of NLRR, propensity score matching (PSM) method was performed to reconfirm the result. A total of 130 patients were enrolled and DICH occurred in 29 patients. Elevated NLRR and history of craniotomy were independent risk factors for DICH secondary to VP shunt. The optimal cut off point of NLRR was 2.05, and the sensitivity was 89.7%, the specificity was 63.4%. Patients with NLRR > 2.05 had much higher incidence of DICH (40.6% vs 4.5%). Our finding suggested that DICH following VP shunt was not a rare complication and elevated NLRR could independently predict DICH. Inflammatory responses might play an important role in the development of DICH following VP shunt.
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http://dx.doi.org/10.1038/s41598-021-93315-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253783PMC
July 2021

Effect of Soil Properties and Aging Time on Oral and Inhalation Bioaccessibility of Copper Oxide Nanoparticles in Soils.

Bull Environ Contam Toxicol 2021 Jun 16. Epub 2021 Jun 16.

School of Water Conservancy and Environment, University of Jinan, Jinan, 250022, China.

In this study, soils spiked with copper oxide nanoparticles (CuO NPs) or Cu(NO) and aged as long as 90 days were utilized to investigate effect of soil properties and aging on oral and inhalation bioaccessibility of CuO NPs. Results showed that oral bioaccessibility of CuO NPs in gastric phase (GP) ranged from 70% to 84%, it significantly decreased to 50%-70% in intestinal phase (IP). The inhalation bioaccessibility of CuO NPs in artificial lysosomal fluid (ALF) ranged from 66% to 85%, and much higher than that in Gamble's solution (GS, 3.3%-23%). By comparing CuO NPs to Cu(NO) bioaccessibility, insignificant difference was found. The aging time (D15 and D90) had limited effect on their oral and inhalation bioaccessibility. CEC and free Al were positively and clay content was negatively correlated with CuO NPs inhalation bioaccessibility, while Cu(NO) inhalation bioaccessibility decreased with increasing soil clay content and pH. Our findings provide an essential basis to evaluate the human health risks of CuO NPs.
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http://dx.doi.org/10.1007/s00128-021-03287-4DOI Listing
June 2021

The effect of non-benzodiazepine sedative hypnotics on CPAP adherence in patients with OSA: a systematic review and meta-analysis.

Sleep 2021 Aug;44(8)

Guangzhou Medical University, State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.

Study Objectives: This meta-analysis aimed to explore the effect of non-benzodiazepine sedative hypnotics (NBSH) on continuous positive airway pressure (CPAP) adherence in patients with obstructive sleep apnea (OSA).

Methods: We conducted a systematic search through PubMed, Medline, the Cochrane Library, EMBASE, Scopus and ClinicalTrials (all searched from inception to August 15, 2020). Publications were limited to articles, clinical conferences and letters, including randomized controlled trials and retrospective studies. We used a random-effects model to calculate the odds ratio (OR) and mean difference (MD) with corresponding confidence interval (CI). Subgroup analyses were conducted to analyze the sources of heterogeneity.

Results: Eight studies fulfilled the inclusion and exclusion criteria for patients newly diagnosed with obstructive sleep apnea. Overall, the use of NBSH was associated with increased use of CPAP per night (MD = 0.62 h; 95% CI = 0.26-0.98) and use for more nights (MD = 12.08%; 95% CI = 5.27-18.88). When a study seriously affecting heterogeneity was removed, more patients adhered well with CPAP use (pooled OR = 2.48; 95% CI = 1.75-3.52) with good adherence defined as CPAP use for >4 h/night on >70% of nights. Among prescribed NBSHs, eszopiclone showed the most significant effect on CPAP adherence.

Conclusion: CPAP adherence may increase in OSA patients treated with non-benzodiazepine sedative hypnotics especially eszopiclone. The effect of zolpidem and zaleplon on CPAP adherence requires further investigation by larger scale, randomized, controlled trials.
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http://dx.doi.org/10.1093/sleep/zsab077DOI Listing
August 2021

Dissolution of copper oxide nanoparticles is controlled by soil solution pH, dissolved organic matter, and particle specific surface area.

Sci Total Environ 2021 Jun 30;772:145477. Epub 2021 Jan 30.

School of Water Conservancy and Environment, University of Jinan, Jinan 250022, China. Electronic address:

Dissolution is the primary process affecting the bioavailability and toxicity of nanoscale copper oxide (nano-CuO) to plants and soil organisms. In this study, particle morphology, organic acid, and soil properties were considered to understand the dissolution characteristics of nano-CuO in soil solutions. The results showed that the copper ions (Cu) released from spherical nano-CuO (CuO NPs), tubular nano-CuO (CuO NTs), and spherical microsized CuO (CuO MPs) in the ten soil solutions were 26.6-4194.0 μg/L, 4.90-217.1 μg/L, and 10.8-326.0 μg/L, respectively. The concentration of Cu was negatively correlated with the pH of the soil solution and positively correlated with the contents of dissolved organic carbon (DOC), aluminum, and manganese. Multivariate stepwise regression analysis indicated that the dissolution of CuO NPs could be well predicted by pH and DOC contents of the soil solutions. In the GD soil solution (acidic), 4- and 8-fold of the DOC content amendments significantly promoted the dissolution of the three sizes of CuOs, resulting in an increase of Cu 4.55-11.3 and 5.67-16.2 times, respectively. In the CQ soil solution (neutral), 8-fold DOC amendments increase the release of Cu 2.13-16.6 times. While in the SD soil solution (alkaline), promoting effect on the dissolution was only observed for nano-CuOs, with Cu elevated by factors of 1.56-4.64 and 1.38-4.48. The amendments of Al and Mn in soil solution increased the amounts of Cu 1.13-4.80 and 1.02-1.46 times in the GD soil solution. In comparison, no significant promoting effects were observed in CQ and SD soil solutions due to their stronger buffering capacities. These findings offer insight into the dissolution behavior of nano-CuOs in soils and be helpful to evaluate their environmental risks.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145477DOI Listing
June 2021

Extraction of lead from electrolytic manganese anode mud by microwave coupled ultrasound technology.

J Hazard Mater 2021 04 3;407:124622. Epub 2020 Dec 3.

School of Metallurgy and Environment, Central South University, Changsha 410083, China.

In this work, extraction of lead from Electrolytic Manganese Anode Mud (EMAM) by microwave coupled ultrasound was studied. The results showed that microwave roasting promoted the conversion of MnO to MnO and MnO, which greatly facilitated the subsequent leaching process of lead and the introduction of ultrasound effectively enhanced the leaching process. The leaching rate for lead from EMAM was arrived to 86.98% under the optimum conditions with the ammonium acetate concentration of 2 mol/L at 343 K and the stirring speed of 300 rpm for 60 min. With the introduction of specific power ultrasound, the leaching of lead was increased by about 10%. The microwave roasting combined with ultrasonic enhanced leaching can effectively for the reuse and reduction of EMAM which provides ideas for further investigation of lead pollution control and resource utilization in EMAM.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124622DOI Listing
April 2021

Clinical risk factor analysis of bilateral vestibular schwannoma's growth pattern inconsistency in individual NF2 patients.

Clin Neurol Neurosurg 2021 01 11;200:106365. Epub 2020 Nov 11.

Department of Neurosurgery, Beijing Tian Tan Hospital, Capital Medical University, Beijing, China. Electronic address:

Objective: Although bilateral vestibular schwannomas (VSs) in individual NF2 patients have the same NF2 gene mutation, they often show different growth patterns. We attempted to identify factors associated with this growth pattern inconsistency.

Patients And Methods: Cranial MR images of 120 untreated VSs in 60 NF2 patients were carefully reviewed for their growth rates. Growth pattern analysis was performed on 68 VSs in 34 NF2 patients followed up for more than three years with at least three cranial MR scans.

Results: Patient age and tumor volume were significantly associated with NF2 VS absolute growth rates (p < 0.05). Bilateral VS growth patterns in individual NF2 patients were the same in 18 (52.9 %) and different in 16 (47.1 %) patients. Patients with consistent bilateral growth patterns were significantly younger than the inconsistent patients (21.8 ± 5.8 years vs. 30.8 ± 13.1 years, p = 0.014). The bilateral VS volume consistency rates were significantly higher in patients with consistent growth patterns than in patients with inconsistent growth patterns (10/18 vs. 3/16, p = 0.028).

Conclusions: Patient age and volume consistency are the clinical risk factors for bilateral NF2 VS growth pattern inconsistencies. Bilateral VSs in young NF2 patients tend to have the same growth patterns. These findings may help us to predict the future clinical behavior of small NF2 VSs based on the past clinical history of the large ones.
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http://dx.doi.org/10.1016/j.clineuro.2020.106365DOI Listing
January 2021

Highly Efficient and Selective Visible-Light Driven CO Reduction by Two Co-Based Catalysts in Aqueous Solution.

Inorg Chem 2020 Dec 8;59(23):17464-17472. Epub 2020 Nov 8.

School of Chemistry and Chemical Engineering, Institute of Materials Science and Engineering, Anhui University of Technology, Maanshan, 243032, People's Republic China.

Photocatalytic CO reduction has been considered as a promising approach to solve energy and environmental problems. Nevertheless, developing inexpensive photocatalysts with high efficiency and selectivity remains a big challenge. In this study, two Co-based complexes [Co(L)Cl] () and [Co(L)Cl] () were synthesized by treating two DPA-based (DPA: dipicolylamine) ligands with Co, respectively. Under visible-light irradiation, the performance of as a homogeneous photocatalyst for CO reduction in aqueous media has been explored by using [Ru(phen)] as a photosensitizer, and triethylolamine (TEOA) as a sacrificial reductant. shows high photocatalytic activity for CO-to-CO conversion, corresponding to the high TON of 2600 and TOF of 260 h (TON = turnover number for CO; TOF = turnover frequency for CO). High selectivity of 97% for CO formation is also achieved. The control experiments catalyzed by demonstrated that two Co(II) centers in may operate independently and activate one CO molecule each. Furthermore, the proposed mechanism of for photocatalytic CO reduction has been investigated via electrochemical analysis, a series of quenching experiments, and density functional theory calculations.
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http://dx.doi.org/10.1021/acs.inorgchem.0c02733DOI Listing
December 2020

The effect of sinomenine eye drops on experimental dry eye in mice.

Cutan Ocul Toxicol 2020 Dec 6;39(4):389-395. Epub 2020 Nov 6.

Department of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.

Objective: To investigate the effect of topical sinomenine (SIN) on ocular surface damage in experimental dry eye in mice.

Material And Methods: Experimental dry eye was created using scopolamine hydrobromide in female C57BL/6 mice. Eye drops consisting of 0.05%, or 0.1% SIN or phosphate-buffered saline (PBS) were applied to the experimental dry eye in mice. Tear product and corneal staining scores were measured at 7 and 14 days after treatment. Interleukin (IL)-1β and tumour necrosis factor (TNF)-α levels in the SIN groups at 14 days after treatment were compared with those of other groups.

Results: Mice treated with 0.05% or 0.1% SIN showed a significant improvement in tear product and corneal irregularity compared to the control and PBS-treated groups. A significant decrease in the levels of IL-1βand TNF-α was observed in the 0.05% and 0.01% SIN-treated groups.

Conclusions: Topical SIN eye drop application can effectively improve clinical signs and decrease inflammation in the ocular surface, and alleviate ocular surface damage in dry eye.
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http://dx.doi.org/10.1080/15569527.2020.1840580DOI Listing
December 2020

Suppression of DDX39B sensitizes ovarian cancer cells to DNA-damaging chemotherapeutic agents via destabilizing BRCA1 mRNA.

Oncogene 2020 11 28;39(47):7051-7062. Epub 2020 Sep 28.

Department of Radiation Medicine, Institute of Systems Biomedicine, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, China.

Multiple RNA processing events including transcription, mRNA splicing, and export are delicately coordinated by the TREX complex. As one of the essential subunits, DDX39B couples the splicing and export machineries by recruiting ALYREF onto mRNA. In this study, we further explore the functions of DDX39B in handling damaged DNA, and unexpectedly find that DDX39B facilitates DNA repair by homologous recombination through upregulating BRCA1. Specifically, DDX39B binds to and stabilizes BRCA1 mRNA. DDX39B ensures ssDNA formation and RAD51 accumulation at DSB sites by maintaining BRCA1 levels. Without DDX39B being present, ovarian cancer cells exhibit hypersensitivity to DNA-damaging chemotherapeutic agents like platinum or PARPi. Moreover, DDX39B-deficient mice show embryonic lethality or developmental retardation, highly reminiscent of those lacking BRCA1. High DDX39B expression is correlated with worse survival in ovarian cancer patients. Thus, DDX39B suppression represents a rational approach for enhancing the efficacy of chemotherapy in BRCA1-proficient ovarian cancers.
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http://dx.doi.org/10.1038/s41388-020-01482-xDOI Listing
November 2020

Decomposition Study of Praseodymium Oxalate as a Precursor for Praseodymium Oxide in the Microwave Field.

ACS Omega 2020 Sep 21;5(34):21338-21344. Epub 2020 Aug 21.

Faculty of Metallurgical and Energy Engineering, State Key Laboratory of Complex Nonferrous Metal Resources Clean Utilization, Kunming Key Laboratory of Special Metallurgy, Kunming University of Science and Technology, Kunming, Yunnan 650093, China.

Micron-sized praseodymium oxide powders are prepared successfully from the praseodymium oxalate in a microwave field at 750 °C for 2 h in the present study. X-ray diffraction (XRD) analysis demonstrates that the presence of cubic structured crystalline PrO and complete decomposition of the precursor are confirmed by Fourier transform infrared (FT-IR) analysis. The scanning electron microscopy (SEM) results show yield powders with the desired particle size and uniform morphologies. Particle size analysis demonstrates that the median diameter ( ) becomes stable at 750 °C. The , average surface area, pore diameter, and pore volume calculated by Brunauer -Emmett-Teller (BET) are 4.32 μm, 6.628 m/g, 1.86 nm, and 0.026 cm/g at 750 °C for 2 h, respectively. Moreover, loss on ignition (L.O.I.) analysis indicates that the L.O.I. is as low as 0.39%, meeting the enterprise requirement (<1%). In comparison, conventional calcination experiments are carried out in the electric furnace. Both XRD and FT-IR analyses are in consistence with thermogravimetry-differential scanning calorimetry, which indicates that the temperature required for the decomposition of praseodymium oxalate hydrate is higher than that of microwave heating. Furthermore, SEM, particle size distribution, and BET analysis indicate that agglomeration generates, particle size enlarges, and average surface area increases. In all, it is confirmed that preparing rare-earth oxides from rare-earth oxalates is feasible using microwave heating to replace conventional heating.
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http://dx.doi.org/10.1021/acsomega.0c00505DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7469113PMC
September 2020

GTree: an Open-source Tool for Dense Reconstruction of Brain-wide Neuronal Population.

Neuroinformatics 2021 04;19(2):305-317

Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong, University of Science and Technology, Hubei, Wuhan, 430074, China.

Recent technological advancements have facilitated the imaging of specific neuronal populations at the single-axon level across the mouse brain. However, the digital reconstruction of neurons from a large dataset requires months of manual effort using the currently available software. In this study, we develop an open-source software called GTree (global tree reconstruction system) to overcome the above-mentioned problem. GTree offers an error-screening system for the fast localization of submicron errors in densely packed neurites and along with long projections across the whole brain, thus achieving reconstruction close to the ground truth. Moreover, GTree integrates a series of our previous algorithms to significantly reduce manual interference and achieve high-level automation. When applied to an entire mouse brain dataset, GTree is shown to be five times faster than widely used commercial software. Finally, using GTree, we demonstrate the reconstruction of 35 long-projection neurons around one injection site of a mouse brain. GTree is also applicable to large datasets (10 TB or higher) from various light microscopes.
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http://dx.doi.org/10.1007/s12021-020-09484-6DOI Listing
April 2021

Impacts of different sources of animal manures on dissemination of human pathogenic bacteria in agricultural soils.

Environ Pollut 2020 Nov 13;266(Pt 2):115399. Epub 2020 Aug 13.

School of Water Conservancy and Environment, University of Jinan, Jinan, 250022, China. Electronic address:

The human pathogenic bacteria (HPB) in animal feces may disseminate to agricultural soils with their land application as organic fertilizer. However, the knowledge about the impacts of different sources and rates of animal manures on the temporal changes of soil HPB remains limited, which hamper our ability to estimate the potential risks of their land application. Here, we constructed an HPB database including 565 bacterial strains. By blasting the 16 S rRNA gene sequences against the database we explored the occurrence and fate of HPB in soil microcosms treated with two rates of swine, poultry or cattle manures. A total of 30 HPB were detected in all of manure and soil samples. Poultry manure at the high level obviously improved the abundance of soil HPB. The application of swine manure could introduce concomitant HPB into the soils. Of which, Pseudomonas syringae pv. syringae B728a and Escherichia coli APEC O78 may deserve more attention because of their survival for a few days in manured soils and being possible hosts of diverse antibiotic resistance genes (ARGs) as revealed by co-occurrence pattern. Bayesian source tracking analysis showed that the HPB derived from swine manure had a higher contribution to soil pathogenic communities than those from poultry or cattle manures in early days of incubation. Mantel test together with variation partitioning analysis suggested that bacterial community and soil physicochemical properties were the dominant factors determining the profile of HPB and contributed 64.7% of the total variations. Overall, our results provided experimental evidence that application of animal manures could facilitate the potential dissemination of HPB in soil environment, which should arouse sufficient attention in agriculture practice and management to avoid the threat to human health.
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http://dx.doi.org/10.1016/j.envpol.2020.115399DOI Listing
November 2020

Ultrasound-assisted cleaning chloride from wastewater using Friedel's salt precipitation.

J Hazard Mater 2021 02 1;403:123545. Epub 2020 Aug 1.

Faculty of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming, Yunnan 650093, China; State Key Laboratory of Complex Nonferrous Metal Resources Clean Utilization, Kunming University of Science and Technology, Kunming, Yunnan 650093, China; Kunming Key Laboratory of Special Metallurgy, Kunming, Yunnan 650093, China. Electronic address:

The chloride salt derived from the rare earth smelting wastewater was effectively dislodged using Friedel's salt precipitation assisted with ultrasonic enhancement. Various single factors such as the reagent ratio, temperatures, reaction time and agitation speed were determined and investigated systematically. Results showed that the optimal single-stage removal efficiency were 88.22% and 80.89% with and without ultrasonic strengthen, respectively. The particle size distribution, morphology and elemental analysis of the precipitation were carried out by TEM, SEM, EDS and XRD analysis. These results revealed that the effect of ultrasonic has been given prominence to the removal efficiency of chloride salt. It is attributed to the cavitation and mechanical disturbance effect of ultrasound. In order to further decline the chloride, a two-stage de-chlorination carried out, the result indicated that the concentration of chloride was 120 mg/L and 430 mg/L with and without ultrasonic strengthening afterwards two-stage de-chlorination, respectively. The chloride concentration can fully meet the effluent concentration requirement under the effect of ultrasonic enhancement.
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http://dx.doi.org/10.1016/j.jhazmat.2020.123545DOI Listing
February 2021

Selection and characterisation of triclosan-specific aptamers using a fluorescence microscope-imaging assay.

Anal Bioanal Chem 2020 Oct 11;412(26):7285-7294. Epub 2020 Aug 11.

School of Biological Sciences, Faculty of Science, Victoria University of Wellington, Wellington, 6140, New Zealand.

This study reports a fluorescence microscope-imaging assay for determining the binding characteristics of single-stranded DNA aptamers selected against the antibacterial agent, triclosan. The imaging assay utilises fluorescently labelled aptamers and target-immobilised matrices. Upon binding of triclosan-specific aptamers to triclosan-conjugated matrices, the binding complex was visualised and the image was captured with the aid of a fluorescence microscope. Subsequently, the fluorescent intensities of aptamer-bound matrices were analysed using dedicated image-processing software and correlated to known concentrations of selected input aptamers. Thus, by plotting fluorescence intensities against different aptamer concentrations, binding isotherms were generated to determine aptamer K values. The imaging assay was applied to characterise the binding affinities and specificities of ten triclosan-specific aptamers H1-H10. One of the candidate aptamers, H6, showed a K value of 378 nM, which was comparable with previously published K values for aptamer-generated against triclosan analogous. In addition, the utility of the imaging assay for aptamer characterisation was compared with a commonly used affinity column-binding assay. It was concluded that the imaging assay was superior to alternative assays in terms of accuracy, simplicity, and reproducibility.
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http://dx.doi.org/10.1007/s00216-020-02863-7DOI Listing
October 2020

Effectiveness of thermal treatment on Pb recovery and Cl removal from sintering dust.

J Hazard Mater 2021 02 2;403:123595. Epub 2020 Aug 2.

State Key Laboratory of Complex Nonferrous Metal Resources Clean Utilization, Kunming University of Science and Technology, Kunming, Yunnan 650093, China; Faculty of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming, Yunnan 650093, China. Electronic address:

Sintering dust is considered as a hazardous waste material owing to its high heavy metal content. In this study, a new technology for the treatment of sintering dust via chlorination roasting was developed. The chlorination behavior of Pb at high temperatures was studied. The volatilization behavior of Pb and Cl was found using different analytical techniques, such as scanning electron microscopy and energy dispersive X-ray spectrometry, X-ray diffraction, X-ray photoelectron spectroscopy, X-ray fluorescence, and atomic absorption spectroscopy. In addition, the thermodynamics and kinetics of the chlorination mechanism of Pb at high temperatures were studied. Thermodynamic analysis results showed that quartz and the chlorinating agent played key roles in the process of chlorination. Compared with KCl and NaCl, CaCl showed superiority in the chlorination. The phase transformation of sintering dust at different temperatures was analyzed. The volatilization rates of Pb and Cl reached 98.69 % and 84.55 %, respectively. The study of kinetics showed that the volatilization of PbCl was controlled by diffusion, and the apparent activation energy is 7.97 kJ/mol. Finally, chlorination roasting residue can be recycled for iron and steel metallurgy.
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http://dx.doi.org/10.1016/j.jhazmat.2020.123595DOI Listing
February 2021

The presence of tetracyclines and sulfonamides in swine feeds and feces: dependence on the antibiotic type and swine growth stages.

Environ Sci Pollut Res Int 2020 Dec 29;27(34):43093-43102. Epub 2020 Jul 29.

Macau Environmental Research Institute, Macau University of Science and Technology, Taipa, Macau, 999078, China.

Swine farms are one of the important sources of antibiotics in the environment. In this study, 42 samples of compound feed and feces of swine collected at different growth stages from intensive farms were evaluated for the occurrence and concentrations of three tetracyclines (TCs, namely oxytetracycline, chlortetracycline, and doxycycline) and three sulfonamides (SAs, namely sulfadiazine, sulfadimidine, and sulfamethoxazole). To check for other additional sources of antibiotic administration, ratios (R) of the measured and the predicted levels of each antibiotic excreted via feces were also estimated. Our results showed that the maximum concentration of TCs was 376,210 μg kg and 541,020 μg kg in the feeds and feces, respectively, both for oxytetracycline. In contrast, the highest concentration of SAs were 16.98 μg kg for sulfadimidine in the feeds and 14.70 μg kg for sulfadiazine in the feces. The concentrations of ΣTCs (sum of the three tetracyclines) in swine feeds and feces were found to be 1-4 orders of magnitude higher than those of ΣSAs (sum of the three sulfonamides). Approximately 36% of the R values were found to be greater than one, indicating other sources of administration such as injection and/or oral administration (via drinking water) may also contribute to the presence of antibiotics in feces. Most of the higher R values were found in starter pigs, which were generally administrated with antibiotics by multiple routes to prevent disease and promote swine growth. Our study suggests that comprehensive measures may be undertaken to control antibiotic use in intensive swine farms.
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http://dx.doi.org/10.1007/s11356-020-10266-5DOI Listing
December 2020

MicroRNA-203a-3p regulates CoCl-induced apoptosis in human retinal pigment epithelial cells by targeting suppressor of cytokine signaling 3.

J Diabetes Complications 2020 10 2;34(10):107668. Epub 2020 Jul 2.

Department of Ophthalmology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China. Electronic address:

Purpose: The apoptosis of human retinal pigment epithelial cells (RPEs) plays a critical role in the pathogenesis of diabetic retinopathy (DR), but the molecular mechanisms remain unclear. In this study, we explored the function of miR-203a-3p in CoCl-induced RPEs apoptosis.

Methods: The cellular localization of miR-203a-3p was assessed by in situ hybridization. Luciferase reporter assays were performed to validate that suppressor of cytokine signaling 3(SOCS3) as a direct target of miR-203a-3p. Effects of miR-203a-3p manipulation on RPEs apoptosis were evaluated using TdT-mediated dUTP Nick-End Labeling (TUNEL) and Flow Cytometry. Expression levels of miR-203a-3p was analyzed by RT-PCR, the expression of target proteins was detected by western blot.

Results: miR-203a-3p was found to be located in the RPE layer of the retinas from normal and diabetic rats and SOCS3 was a direct target of miR-203a-3p. miR-203a-3p mimics resulted in improved CoCl-induced apoptosis of RPEs, overexpression of SOCS3 or c-Jun N-terminal kinase (JNK) inhibitor SP600125 reversed the pro-apoptotic effect of miR-203a-3p, to a certain extent.

Conclusions: Our data implied a crucial role of miR-203a-3p as a novel regulator of CoCl-induced RPEs apoptosis through SOCS3. Deregulation of miR-203a-3p/SOCS3/JNK/c-Jun cascade thus may serve as an important contributor to RPEs apoptosis in DR.
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http://dx.doi.org/10.1016/j.jdiacomp.2020.107668DOI Listing
October 2020

3D high resolution generative deep-learning network for fluorescence microscopy imaging.

Opt Lett 2020 Apr;45(7):1695-1698

Microscopic fluorescence imaging serves as a basic tool in many research areas including biology, medicine, and chemistry. With the help of optical clearing, large volume imaging of a mouse brain and even a whole body has been enabled. However, constrained by the physical principles of optical imaging, volume imaging has to balance imaging resolution and speed. Here, we develop a new, to the best of our knowledge, 3D deep learning network based on a dual generative adversarial network (dual-GAN) framework for recovering high-resolution (HR) volume images from high speed acquired low-resolution (LR) volume images. The proposed method does not require a precise image registration process and meanwhile guarantees the predicted HR volume image faithful to its corresponding LR volume image. The results demonstrated that our method can recover ${20} {\times} /1.0\text-{\rm NA}$20×/1.0-NA volume images from coarsely registered ${5} {\times} /0.16\text-{\rm NA}$5×/0.16-NA volume images collected by light-sheet microscopy. This method would provide great potential in applications which require high resolution volume imaging.
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http://dx.doi.org/10.1364/OL.387486DOI Listing
April 2020

CCAAT/Enhancer-Binding Protein Mediates Oxygen-Induced Retinal Neovascularization via Retinal Vascular Damage and Vascular Endothelial Growth Factor.

J Diabetes Res 2020 9;2020:2789209. Epub 2020 Mar 9.

Department of Ophthalmology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China.

Objective: To evaluate the role of CCAAT/enhancer-binding protein (C/EBP (C/EBP.

Methods: Rats with OIR were exposed to alternating hypoxic and hyperopic conditions for 14 days. Then, the rats with OIR were assigned randomly to groups that received intravitreal injections of either shRNA lentiviral particles targeting C/EBP (C/EBP (C/EBP (C/EBP (C/EBP (C/EBP (C/EBP.

Results: In OIR rats, the expression levels of C/EBP (C/EBP < 0.01). The p-C/EBP (C/EBP (C/EBP (C/EBP (C/EBP (C/EBP < 0.01). The p-C/EBP (C/EBP (C/EBP (C/EBP < 0.01). The p-C/EBP.

Conclusions: C/EBP shRNA inhibits RNV in OIR. A potential mechanism may be that the activity of C/EBP increases with its overexpression, which in turn aggravates the amount of the retinal vascular damage and promotes transcription of VEGF. C/EBP might be a new therapeutic target for preventing RNV. (C/EBP (C/EBP (C/EBP.
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http://dx.doi.org/10.1155/2020/2789209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7085405PMC
January 2021

Knockdown Of Long Non-Coding RNA TP73-AS1 Inhibited Cell Proliferation And Metastasis Through Wnt/β-Catenin Pathway In Lung Adenocarcinoma.

Onco Targets Ther 2019 13;12:9599-9610. Epub 2019 Nov 13.

Department of Clinical Laboratory, Liyang People's Hospital, Liyang 213300, People's Republic of China.

Background: Various evidences showed that abnormally expressed long non-coding RNAs (lncRNAs) play important roles in the tumorigenesis and progression of malignancies. However, the exact role and regulatory mechanism of lncRNA TP73-AS1 in the pathogenesis and progression of lung adenocarcinoma remain to be further elucidated.

Purpose: The aim of this study was to investigate the functional role and underlying mechanism of lncRNA TP73-AS1 in lung adenocarcinoma progression.

Methods: RT-PCR assay was employed to detect TP73-AS1 expression in lung adenocarcinoma tissues and cells. The function of TP73-AS1 in lung adenocarcinoma progression was estimated by MTT assay, EdU assay, flow cytometry, Western blot, wound-healing assay and transwell assay.

Results: LncRNA TP73-AS1 expression was significantly increased in lung adenocarcinoma tissues and cell lines. Moreover, functional assays revealed that silencing of lncRNA TP73-AS1 could attenuate cell proliferation, migration, invasion and epithelial-mesenchymal transition of lung adenocarcinoma, while enhanced expression of lncRNA TP73-AS1 led to the opposite results. Additionally, lncRNA TP73-AS1 knockdown could facilitate cell apoptosis and overexpression of lncRNA TP73-AS1 inhibited cell apoptosis. In addition, we further determined that lncRNA TP73-AS1 regulated cell metastasis through inducing the activation of Wnt/β-catenin signaling pathway in lung adenocarcinoma.

Conclusion: Our results indicated that lncRNA TP73-AS1 may play an oncogenic role in lung adenocarcinoma progression, which provided a promising therapy strategy for the treatment of lung adenocarcinoma.
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http://dx.doi.org/10.2147/OTT.S215543DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6859124PMC
November 2019

Characterization of global 5-hydroxymethylcytosine in pediatric posterior fossa ependymoma.

Clin Epigenetics 2020 01 28;12(1):19. Epub 2020 Jan 28.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, 119# South 4th Ring Road, Fengtai District, Beijing, 100070, China.

Background: 5-Hydroxymethylcytosine (5hmC) is a novel epigenetic mark and may be involved in the mechanisms of tumorigenesis and malignant transformation. However, the role of 5hmC in ependymoma, the third most common brain tumor in children, remains unclear. The aim of this study sought to identify the characterization of 5hmC levels in pediatric posterior fossa ependymoma and to evaluate whether 5hmC levels could be a potential factor to predict clinical outcomes.

Results: Our results showed that 5hmC levels were globally decreased in posterior fossa ependymoma compared with normal cerebellum tissues (P < 0.001). Group A posterior fossa ependymomas had higher 5hmC levels than group B tumors (P = 0.007). Moreover, 5hmC levels positively correlated with Ki-67 index in posterior fossa ependymoma (r = 0.428, P = 0.003). Multivariate Cox hazards model revealed that patients with high 5hmC levels (> 0.102%) had worse PFS and OS than patients with lower 5hmC levels (< 0.102%) (PFS: HR = 3.014; 95% CI, 1.040-8.738; P = 0.042; OS: HR = 2.788; 95% CI, 0.974-7.982; P = 0.047).

Conclusions: Our findings suggest that loss of 5hmC is an epigenetic hallmark for pediatric posterior fossa ependymoma. 5hmC levels may represent a potential biomarker to predict prognosis in children with posterior fossa ependymoma.
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http://dx.doi.org/10.1186/s13148-020-0809-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6988368PMC
January 2020

Effectiveness of microwave-assisted thermal treatment in the extraction of gold in cyanide tailings.

J Hazard Mater 2020 02 17;384:121456. Epub 2019 Oct 17.

State Key Laboratory of Complex Nonferrous Metal Resources Clean Utilization, Kunming University of Science and Technology, Kunming, Yunnan, 650093, China; Faculty of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming, Yunnan, 650093, China; Key Laboratory of Unconventional Metallurgy, Ministry of Education, Kunming, Yunnan, 650093, China; National Local Joint Laboratory of Engineering Application of Microwave Energy and Equipment Technology, Kunming, Yunnan, 650093, China.

A new technology for treating cyanide tailings (CT) by microwave chlorination roasting was first proposed in this study. A green process with prospective environmental and economic significance was experimentally and theoretically established for the sustainable extraction of gold from CT. The microwave roasting behavior and trajectory of gold in different gold-bearing bodies under microwave-enhanced roasting and conventional roasting conditions were explored and compared by introducing the concept of thermal and non-thermal effects provided by the microwave field. At the same time, the superiority of microwave chlorination roasting was verified by a series of experiments. Under the same conditions of the roasting experiments, the energy consumption of conventional calcination was more than double greater than that of microwave roasting. Finally, the essence of microwave chlorination roasting in the treatment of CT was summarized as a non-polluting process.
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http://dx.doi.org/10.1016/j.jhazmat.2019.121456DOI Listing
February 2020

Ultrasonic-enhanced replacement of lead in lead hydrometallurgy process from lead leaching solution.

R Soc Open Sci 2019 Jul 10;6(7):190042. Epub 2019 Jul 10.

School of Metallurgy, Northeastern University, Shenyang, Liaoning 110819, People's Republic of China.

In this paper, ultrasonic-enhanced replacement of lead by zinc in lead leaching solution was studied. The effects of reaction time, rotational speed, temperature, concentration of leaching solution and the ratio of the surface area of the zinc plate immersed in the leaching solution to the volume of leaching solution (S : V) were studied under both conventional and ultrasonic conditions. The optimum ultrasonic-assisted replacement conditions were as follows: the S : V of 0.04 (4 cm 100 ml), reaction temperature of 30°C, replacement time of 30 min and the concentration of leaching solution is 5 g l, leading to a lead replacement rate of 94.84%. Compared with the conventional replacement process, the reaction time of ultrasonic-enhanced substitution could be reduced to one half, and the demand of reaction temperature, leaching solution concentration and other conditions were decreased accordingly. Introducing ultrasonic into the replacement reaction is promising to reduce the energy consumption in the hydrometallurgical industry also caters to the demands of environment protection.
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http://dx.doi.org/10.1098/rsos.190042DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6689618PMC
July 2019

Brain-Wide Shape Reconstruction of a Traced Neuron Using the Convex Image Segmentation Method.

Neuroinformatics 2020 04;18(2):199-218

Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, 430074, Hubei, China.

Neuronal shape reconstruction is a helpful technique for establishing neuron identity, inferring neuronal connections, mapping neuronal circuits, and so on. Advances in optical imaging techniques have enabled data collection that includes the shape of a neuron across the whole brain, considerably extending the scope of neuronal anatomy. However, such datasets often include many fuzzy neurites and many crossover regions that neurites are closely attached, which make neuronal shape reconstruction more challenging. In this study, we proposed a convex image segmentation model for neuronal shape reconstruction that segments a neurite into cross sections along its traced skeleton. Both the sparse nature of gradient images and the rule that fuzzy neurites usually have a small radius are utilized to improve neuronal shape reconstruction in regions with fuzzy neurites. Because the model is closely related to the traced skeleton point, we can use this relationship for identifying neurite with crossover regions. We demonstrated the performance of our model on various datasets, including those with fuzzy neurites and neurites with crossover regions, and we verified that our model could robustly reconstruct the neuron shape on a brain-wide scale.
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http://dx.doi.org/10.1007/s12021-019-09434-xDOI Listing
April 2020

C1QBP Promotes Homologous Recombination by Stabilizing MRE11 and Controlling the Assembly and Activation of MRE11/RAD50/NBS1 Complex.

Mol Cell 2019 09 25;75(6):1299-1314.e6. Epub 2019 Jul 25.

Department of Radiation Medicine, Institute of Systems Biomedicine, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China. Electronic address:

MRE11 nuclease forms a trimeric complex (MRN) with RAD50 and NBS1 and plays a central role in preventing genomic instability. When DNA double-strand breaks (DSBs) occur, MRN is quickly recruited to the damage site and initiates DNA end resection; accordingly, MRE11 must be tightly regulated to avoid inefficient repair or nonspecific resection. Here, we show that MRE11 and RAD50 form a complex (MRC) with C1QBP, which stabilizes MRE11/RAD50, while inhibiting MRE11 nuclease activity by preventing its binding to DNA or chromatin. Upon DNA damage, ATM phosphorylates MRE11-S676/S678 to quickly dissociate the MRC complex. Either excess or insufficient C1QBP impedes the recruitment of MRE11 to DSBs and impairs the DNA damage response. C1QBP is highly expressed in breast cancer and positively correlates with MRE11 expression, and the inhibition of C1QBP enhances tumor regression with chemotherapy. By influencing MRE11 at multiple levels, C1QBP is, thus, an important player in the DNA damage response.
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http://dx.doi.org/10.1016/j.molcel.2019.06.023DOI Listing
September 2019

Land Use Change and Climate Variation in the Three Gorges Reservoir Catchment from 2000 to 2015 Based on the Google Earth Engine.

Sensors (Basel) 2019 May 7;19(9). Epub 2019 May 7.

Research Base of Karst Eco-Environments at Nanchuan in Chongqing, Ministry of Nature Resources, Chongqing Key Laboratory of Karst Environment, School of Geographical Sciences, Southwest University, Chongqing 400715, China.

Possible environmental change and ecosystem degradation have received increasing attention since the construction of Three Gorges Reservoir Catchment (TGRC) in China. The advanced Google Earth Engine (GEE) cloud-based platform and the large number of Geosciences and Remote Sensing datasets archived in GEE were used to analyze the land use and land cover change (LULCC) and climate variation in TGRC. GlobeLand30 data were used to evaluate the spatial land dynamics from 2000 to 2010 and Landsat 8 Operational Land Imager (OLI) images were applied for land use in 2015. The interannual variations in the Land Surface Temperature (LST) and seasonally integrated normalized difference vegetation index (SINDVI) were estimated using Moderate Resolution Imaging Spectroradiometer (MODIS) products. The climate factors including air temperature, precipitation and evapotranspiration were investigated based on the data from the Global Land Data Assimilation System (GLDAS). The results indicated that from 2000 to 2015, the cultivated land and grassland decreased by 2.05% and 6.02%, while the forest, wetland, artificial surface, shrub land and waterbody increased by 3.64%, 0.94%, 0.87%, 1.17% and 1.45%, respectively. The SINDVI increased by 3.209 in the period of 2000-2015, while the LST decreased by 0.253 °C from 2001 to 2015. The LST showed an increasing trend primarily in urbanized area, with a decreasing trend mainly in forest area. In particular, Chongqing City had the highest LST during the research period. A marked decrease in SINDVI occurred primarily in urbanized areas. Good vegetation areas were primarily located in the eastern part of the TGRC, such as Wuxi County, Wushan County, and Xingshan County. During the 2000-2015 period, the air temperature, precipitation and evapotranspiration rose by 0.0678 °C/a, 1.0844 mm/a, and 0.4105 mm/a, respectively. The climate change in the TGRC was influenced by LULCC, but the effect was limited. What is more, the climate change was affected by regional climate change in Southwest China. Marked changes in land use have occurred in the TGRC, and they have resulted in changes in the LST and SINDVI. There was a significantly negative relationship between LST and SINDVI in most parts of the TGRC, especially in expanding urban areas and growing forest areas. Our study highlighted the importance of environmental protection, particularly proper management of land use, for sustainable development in the catchment.
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http://dx.doi.org/10.3390/s19092118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6540281PMC
May 2019

Deletion of Brg1 causes stereocilia bundle fusion and cuticular plate loss in vestibular hair cells.

Hear Res 2019 06 8;377:247-259. Epub 2019 Apr 8.

School of Life Science and Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong University, Jinan, 250100, China. Electronic address:

Brg1 is an ATPase subunit of the SWI/SNF chromatin-remodeling complex, and it is indispensable for the development and homeostasis of various organs. Conditional deletion of Brg1 in cochlea hair cells (HCs) leads to multiple structural defects and profound deafness. However, the premature death of Brg1-deficient cochlea HCs hindered further study of the role of Brg1. In contrast to cochlea HCs, Brg1-deficient vestibular HCs survived for a long time. Therefore, HC apical structure and vestibular function were examined in inner HC-specific conditional Brg1 knockout mice. Vestibular HCs exhibited fused and elongated stereocilia bundles after deletion of Brg1, and the cuticular plate was absent in most HCs with fused stereocilia bundles. HC loss was observed in conditional Brg1 knockout mice at the age of 12 months. Morphological defects and HC loss were primarily restricted in the striolar region of the utricle and saccule and in the central region of ampulla. The behavioral tests revealed that Brg1 deletion in HCs caused vestibular dysfunction in older adult mice. These results suggest that Brg1 may play specific roles in the maintenance of the HC stereocilia bundle and the cuticular plate.
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http://dx.doi.org/10.1016/j.heares.2019.04.002DOI Listing
June 2019
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