Publications by authors named "Rong Xiao"

525 Publications

A Deep Learning-based Smartphone Platform for Cutaneous Lupus Erythematosus Classification Assistance-Simplifying the Diagnosis of Complicated Diseases.

J Am Acad Dermatol 2021 Feb 18. Epub 2021 Feb 18.

Department of Dermatology, Second Xiangya Hospital, Central South University, Hunan Key Laboratory of Medical Epigenomics, Changsha, Hunan, China; Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College. Electronic address:

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http://dx.doi.org/10.1016/j.jaad.2021.02.043DOI Listing
February 2021

Ozonation Treatment Increases Chlorophenylacetonitrile Formation in Downstream Chlorination or Chloramination.

Environ Sci Technol 2021 Feb 17. Epub 2021 Feb 17.

State Key Laboratory of Pollution Control and Resources Reuse, Key Laboratory of Yangtze River Water Environment, Ministry of Education, International Joint Research Center for Sustainable Urban Water System, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.

Chlorophenylacetonitriles (CPANs) are an emerging group of aromatic nitrogenous disinfection byproducts (DBPs). However, their dominant precursors and formation pathways remain unclear, which hinders the further development of effective control strategies. For the first time, CPAN precursors were screened by conducting formation potential (FP) tests on real water samples from six drinking water treatment plants (DWTPs). The average overall removal of CPAN precursors across all six DWTPs was only 10%. Moreover, ozonation increased CPAN precursors by 140% on average. Fluorescence spectroscopy showed a dramatic reduction in aromatic proteins, tyrosine-like proteins, and tryptophan-like proteins following ozonation. Low-apparent-molecular-weight (AMW) (<1 kDa) substances were correlated with the CPAN FP in these samples. We therefore hypothesized that protein fragments with low AMW, such as amino acids, are important CPAN precursors during downstream chlor(am)ination. Two aromatic free amino acids, tyrosine and tryptophan, were selected to investigate the formation of CPANs during chlor(am)ination. Both amino acids were found to act as CPAN precursors for the first time. CPAN formation pathways from these model precursors were proposed based on the frontier molecular orbital theory and intermediate products identified using high-resolution mass spectrometry. This study provides a powerful theoretical foundation for controlling CPAN formation in drinking water.
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http://dx.doi.org/10.1021/acs.est.0c07853DOI Listing
February 2021

Applicability of the Dual-Factor Model of Mental Health in the Mental Health Screening of Chinese College Students.

Front Psychol 2020 18;11:549036. Epub 2021 Jan 18.

Department of Psychology, School of Public Health, Southern Medical University, Guangzhou, China.

Traditional mental health models focus on psychopathological symptoms. In contrast, a dual-factor model of mental health integrates psychopathology and subjective well-being into a mental health continuum, and it is adjustment and supplement for traditional mental health research paradigm. The present study explores the applicability of a dual-factor model of mental health in mental health screening of Chinese college students. To assess mental health statuses of 2,065 college students, we used Flourishing Scale Chinese Version, Satisfaction With Life Scale, the seven-item Patient Health Questionnaire, the Mental Health Continuum-Short Form, and Purpose in Life Test-Short Form. Results showed that the dual-factor model of mental health has a good fit index. Also, a feasible screening scale was addressed. The results indicate the importance of addressing both subjective well-being and psychopathology in evaluating mental health screening of college students.
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http://dx.doi.org/10.3389/fpsyg.2020.549036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874216PMC
January 2021

Risk factors of postoperative delirium in the knee and hip replacement patients: a systematic review and meta-analysis.

J Orthop Surg Res 2021 Jan 22;16(1):76. Epub 2021 Jan 22.

Department of Orthopedics, West China Hospital/West China School of Medicine, Sichuan University, 37# Wuhou Guoxue road, Chengdu, 610041, People's Republic of China.

Background: The risk factors of postoperative delirium (POD), a serious while preventable complication, developed by patients undergoing knee and replacement surgery are still under investigation. In this systematic review and meta-analysis, we identified risk factors associated with POD in knee and hip replacement.

Methods: PubMed, Ovid MEDLINE, and Ovid EMBASE were used to identify original researches. The studies evaluating the risk factors of POD after knee and hip replacement were reviewed, and the qualities of the included studies were assessed with Newcastle-Ottawa Scale. Data were extracted, pooled, and a meta-analysis was completed RESULT: Twenty-two studies were finally included with a total of 11934 patients who underwent knee or hip replacement and 1841 developed POD with an incidence of 17.6% (95% confidential interval (CI) 13.2-22.0%). Eighteen significant risk factors were identified including advanced age (odds ratio (OR) 1.15 95% CI 1.08-1.22), cognitive impairment (OR 6.84, 95% CI 3.27-14.33), history of cerebrovascular events (OR 2.51, 95% CI 1.28-4.91), knee replacement (OR 1.42, 95% CI 1.00-2.02), blood loss (standardized mean difference (SMD) 0.30, 95% CI 0.15-0.44), dementia (OR 3.09, 95% CI 2.10-4.56), neurologic disorders (OR 2.26, 95% CI 1.23-4.15), psychiatric illness (OR 2.74, 95% CI 1.34-5.62), and obstructive sleep apnea (OR 4.17, 95% CI 1.72-10.09) along with several comorbidity evaluation scores and laboratory markers.

Conclusion: We identified risk factors consistently associated with the incidence of POD in knee and hip replacement. Strategies and interventions should be implemented to the patients receiving knee or hip replacement with potential risk factors identified in this meta-analysis.
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http://dx.doi.org/10.1186/s13018-020-02127-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821501PMC
January 2021

Targeting the IRAK1-S100A9 Axis Overcomes Resistance to Paclitaxel in Nasopharyngeal Carcinoma.

Cancer Res 2021 Jan 5. Epub 2021 Jan 5.

Deptment of Nasopharyngeal Carcinoma, Sun Yat-sen University Cancer Center;State Key Laboratory of Oncology in South China; Collaborative Innovation Center for Cancer Medicine, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagno

Novel strategies to treat late-stage nasopharyngeal carcinoma (NPC) that often develop resistance to chemotherapy remains an unmet clinical demand. In this study, we identify the multi-kinase inhibitor pacritinib as capable of re-sensitizing the response to paclitaxel in an acquired resistance model. Transcriptome analysis of paclitaxel-sensitive and -resistant cell lines, as well as chemorefractory clinical samples identified S100A9 as the top candidate gene suppressed by pacritinib and whose overexpression was significantly associated with paclitaxel resistance and poor clinical outcome. Moreover, both paclitaxel-resistant NPC cells and relapsed/metastatic clinical samples exhibited increased IRAK1 phosphorylation and that pacritinib could abolish the IRAK1 phosphorylation to suppress S100A9 expression. Functional studies in both in vitro and in vivo models showed that genetic or pharmacological blockade of IRAK1 overcame the resistance to paclitaxel, and combined treatment of pacritinib with paclitaxel exhibited superior anti-tumor effect. Together, these findings demonstrated an important role of the IRAK1-S100A9 axis in mediating resistance to paclitaxel. Furthermore, targeting IRAK1 by pacritinib, may provide a novel therapeutic strategy to overcome chemoresistance in NPC.
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http://dx.doi.org/10.1158/0008-5472.CAN-20-2125DOI Listing
January 2021

Changes in soil microbial community composition during Phragmites australis straw decomposition in salt marshes with freshwater pumping.

Sci Total Environ 2021 Mar 8;762:143996. Epub 2020 Dec 8.

School of Ecology and Nature Conservation, Beijing Forestry University, Beijing 100083, China. Electronic address:

The dynamic changes of soil microorganisms after Phragmites australis straw addition in the incubation tubes were analyzed by phospholipid fatty acid stable isotope probing (PLFA-SIP). After comparing soils from different freshwater pumping areas in the Yellow River Estuary (10-year pumping area, 15-year pumping area and natural salt marsh without pumping), the results showed that the total PLFA contents significantly increased by 59.99%-146.93% after the addition of straw to surface soils (0-10 cm) in the pumping areas, whereas the changes in deeper soils (10-20 cm) were not significant. In particular, the PLFA results showed that bacteria and fungi were significantly increased after 10 days with straw addition. Straw treatment also improved the ratio of fungi to bacteria (F:B) in the surface soils of all sampling sites. The soil microorganisms directly absorbed straw-derived C, where Gram-negative bacteria (GN) were found to have the highest PLFA-C values during the 40-day decomposition process. Soil characteristics can significantly affect microbial community composition. Accordingly, soil organic carbon (SOC) was found to be significantly positively related to bacterial, fungal and other microbial biomasses, while moisture, electric conductivity (EC) and soil aggregate composition were important factors of influence on the microbial community. The findings indicated that both fungi and bacteria were essential microbial communities in straw decomposition, the significant increase of fungi biomass and the absorption of straw-derived C by bacteria were the main changes of microbial community. Long-term freshwater pumping can promote straw decomposition by increasing microbial biomass and changing microbial community composition.
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http://dx.doi.org/10.1016/j.scitotenv.2020.143996DOI Listing
March 2021

Rejection of chlorinated, brominated, and iodinated trihalomethanes by multi-stage reverse osmosis: Efficiency and mechanisms.

Chemosphere 2021 Apr 15;268:129307. Epub 2020 Dec 15.

State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China. Electronic address:

Reverse osmosis (RO), a promising technology for removing inorganic salts and a wide range of trace organic pollutants, is widely used in water treatment industry. In this study, the rejection of chlorinated, brominated, and iodinated trihalomethanes (THMs) by a multi-stage RO system was investigated. The results showed that the multi-stage RO system is effective in rejecting THMs, and THMs with large size, high hydrophobicity and low polarity were highly rejected. In the first stage, high percentage of THMs was adsorbed on RO membrane, and the THM rejection was dominated by both hydrophobic adsorption and size exclusion. The contribution of hydrophobic adsorption to THM rejection decreased significantly along RO stages due to decreased feed concentration, but the enhancement of size exclusion still ensured high rejection efficiencies for most THMs, indicating a compensation effect between two rejection mechanisms. Finally, to further understand the rejection in the multi-RO system from a perspective of THM property, multiple linear regression models were built. The impact of n-octanol-water partition coefficient (Log K) was slightly higher than that of stokes radius in the first stage, which was consistent with the rejection mechanism. But dipole moment played an increasingly important role in the second and third stage, weakening the impact of Log K on THM rejection.
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http://dx.doi.org/10.1016/j.chemosphere.2020.129307DOI Listing
April 2021

Management strategies for dermatomyositis during the coronavirus disease 2019 outbreak.

Clin Dermatol 2020 Nov - Dec;38(6):731-733. Epub 2020 Aug 5.

Department of Dermatology, The Second Xiangya Hospital of Central South University, Changsha, China. Electronic address:

In late 2019, the coronavirus disease 2019 (COVID-19) broke out in Wuhan and then spread over China, which greatly affected the medical practices and health care systems. With most of the hospital's outpatient services closed, the routine clinical diagnosis and treatment for patients with dermatomyositis has been disturbed. We conducted telephone follow-up for 52 patients to know the changes in the condition and the continuation of drug therapy and to ensure the continuity, safety, and effectiveness of the treatment of patients with dermatomyositis during COVID-19.
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http://dx.doi.org/10.1016/j.clindermatol.2020.08.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7403873PMC
December 2020

27-Hydroxycholesterol Promotes the Transfer of Astrocyte-Derived Cholesterol to Neurons in Co-cultured SH-SY5Y Cells and C6 Cells.

Front Cell Dev Biol 2020 24;8:580599. Epub 2020 Nov 24.

School of Public Health, Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, China.

Abnormality in cholesterol homeostasis in the brain is a feature of Alzheimer's disease (AD). 27-Hydroxycholesterol (27-OHC) has been identified as a possible biomarker of AD, but its effects on cholesterol metabolism have not been fully characterized. This study was aimed to investigate the impacts of 27-OHC on cholesterol metabolism in nerve cells. SH-SY5Y cells and C6 cells were co-cultured and treated with 5, 10, and 20 μM 27-OHC for 24 h. Results showed that 27-OHC decreased cholesterol levels and up-regulated the expression of transport-related proteins in C6 cells. In SH-SY5Y cells, 27-OHC increased cholesterol accumulation, especially on plasma membrane (PM), which was consistent with the up-regulation of expressions of cholesterol endocytosis receptors, lipid raft-related proteins, and cholesterol esterase. Simultaneously, accumulation of membrane cholesterol promoted cholesterol conversion to 24S-OHC by CYP46A1(24S-hydroxylase) transfer from the endoplasmic reticulum (ER) to PM. Besides, Aβ levels were elevated in SH-SY5Y cells after 27-OHC treatment. Our results suggest that 27-OHC motivates the transfer of astrocyte-derived cholesterol to neurons. Although there exists a feedback mechanism that excessive cholesterol promotes its conversion to 24S-OHC, the increased cholesterol induced by 27-OHC could not be wholly offset in neurons.
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http://dx.doi.org/10.3389/fcell.2020.580599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732486PMC
November 2020

Identification of the ceRNA networks in α-MSH-induced melanogenesis of melanocytes.

Aging (Albany NY) 2020 12 14;13(2):2700-2726. Epub 2020 Dec 14.

Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha 410013, Hunan, China.

α-MSH is known for melanogenesis stimulation, and ceRNA is a new method involved in physiological regulation. However, whether ceRNA participates in α-MSH-induced melanogenesis remains unknown. We used ceRNA array to detect the expression profiles of lncRNAs, circRNAs, and mRNAs in melanocytes after α-MSH treatment. Moreover, the melanogenesis-related ceRNA regulatory networks were screened and validated. The expression profile analysis showed that 20 lncRNAs and 49 circRNAs changed five-fold after α-MSH treatment, while 933 mRNAs changed two-fold. Based on differentially expressed genes, GO and KEGG analysis were conducted and revealed that 14 genes were enriched in melanogenesis. Then, multiple lncRNA or circRNA-miRNA-mRNA ceRNA networks and lncRNA/circRNA-miRNA-mRNA quaternary ceRNA networks were identified. Thereinto, ENST00000606533, circ_0091223, and TYR expression were upregulated in α-MSH-treated melanocytes, while their complementary miR-1291 was decreased. Dual-luciferase reporter assay further verified that ENST00000606533 and circ_0091223 could bind to miR-1291. ENST00000606533 and circ_0091223 siRNAs decreased circ_0091223, ENST00000606533, and TYR expression, but increased miR-1291 expression. Conversely, miR-1291 mimics inhibited ENST00000606533, circ_0091223, and TYR expression. Moreover, miR-1291 inhibitor could reverse the inhibitory effect of the two siRNAs on TYR expression. Hence, the "ENST00000606533/circ_0091223-miR-1291-TYR" ceRNA network is involved in α-MSH-induced melanogenesis, and ceRNA networks may be potential therapeutic targets for skin pigmentation disorders.
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http://dx.doi.org/10.18632/aging.202320DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7880406PMC
December 2020

Effects of hydroxyl, carboxyl, and amino functionalized carbon nanotubes on the functional diversity of microbial community in riverine sediment.

Chemosphere 2021 Jan 22;262:128053. Epub 2020 Aug 22.

College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, 410082, PR China.

Nowadays, more and more attention is focused on the environmental harm brought by the wide production and use of carbon nanotubes. In this study, the metabolic function of sediment microbial community was investigated after unfunctionalized or functionalized multi-walled carbon nanotubes (MWCNTs) were incorporated. The surface functional groups on the studied functionalized MWCNTs in this work were hydroxyl, carboxyl, and amino, respectively. The metabolic functional diversity was determined by Biolog EcoPlates after one-month exposure to MWCNTs. Incorporating 0.5 wt% amino functionalized MWCNTs significantly decreased the microbial activity and diversity, and all types of MWCNTs caused great inhibition on the microbial metabolism at the dosage of 2.0 wt%. The sediment microbes preferred polymers and amino acids. Principal component and similarity analysis indicated that the microbial carbon metabolism was more affected by the MWCNT dosage compared with the functionalization, and 2.0 wt% amino functionalized MWCNTs made the greatest difference in metabolic function of sediment microbial community. These consequences may help to assess the environmental risks of MWCNTs from the aspect of ecological relevance of sediment microbial community.
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http://dx.doi.org/10.1016/j.chemosphere.2020.128053DOI Listing
January 2021

The effects of high-density lipoprotein and oxidized high-density lipoprotein on forskolin-induced syncytialization of BeWo cells.

Placenta 2021 Jan 23;103:199-205. Epub 2020 Oct 23.

School of Public Health, Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, 100069, China. Electronic address:

Introduction: The negative relationship between maternal high-density lipoprotein-cholesterol (HDL-c) level during pregnancy and infant birth weight has been found. Syncytialization (differentiation and fusion) of trophoblast cells is important to fetal development. HDL has an antioxidant effect, and has been proved to protect trophoblast functions including hormone secretion and invasion. However, HDL is susceptible to oxidation, and high concentrations of HDL impair cell growth and oxidized HDL (oxHDL) inhibits cell proliferation and migration. Moreover, the effects of HDL and oxHDL on trophoblast syncytialization have not been characterized. The aim of this study was to investigate the effects of HDL and oxHDL on trophoblast syncytialization.

Methods: Human choriocarcinoma trophoblasts (BeWo cells) were treated with human HDL or oxHDL and then induced to differentiate by forskolin in syncytialization assays. Expression levels of mRNAs and proteins regulating syncytialization were detected by real-time PCR and western blotting, respectively.

Results: Treatments of HDL at high concentrations reduced human chorionic gonadotropin (hCG) secretion, placental alkaline phosphatase activity and fusion rates, and decreased the expressions of GCM1 and ERVW-1 mRNA as well as phospho-MAPK1/3 (p-MAPK1/3) and total MAPK1/3 protein in the forskolin-induced syncytialization of BeWo cells. Furthermore, treatment of oxHDL (20 μg/ml) decreased hCG secretion, but increased the expression of p-MAPK1/3 protein.

Discussion: These data suggested that both HDL at high concentrations and oxHDL inhibited BeWo cells syncytialization, and might be harmful to placental and fetal development.
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http://dx.doi.org/10.1016/j.placenta.2020.10.024DOI Listing
January 2021

Formation and degradation mechanisms of CXR-type oxidation by-products during cobalt catalyzed peroxymonosulfate oxidation: The roles of Co and SO.

J Hazard Mater 2021 Mar 12;405:124243. Epub 2020 Oct 12.

State Key Laboratory of Pollution Control and Resources Reuse, Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China. Electronic address:

Sulfate radical (SO)-based advanced oxidation processes (AOPs) attract increasing attention in the control of micropollutants. However, SO can react with other chemicals present in water and result in undesired oxidation by-products (OBPs) generation. The formation and degradation mechanisms of CXR-type OBPs during cobalt catalyzed peroxymonosulfate (Co/PMS) oxidation were investigated. In the formation of CXR-type OBPs, both Co and SO could convert chloride to free chlorine that then reacted with natural organic matter, leading to the formation of CXR-type OBPs. The concentrations of trichloromethane, chloral hydrate, dichloroacetonitrile, dichloroacetamide and trichloroacetamide after 15 min reaction were 9.8, 3.9, 1.2, 5.9 and 22.3 nM, respectively. Compared to SO, Co played a more significant role in the CXR-type OBP formation and calculated toxicity values of CXR-type OBPs. CXR-type OBPs could not only be formed but also be degraded at the same time during Co/PMS oxidation. As for the degradation of CXR-type OBPs, both Co and SO could transform CXR-type OBPs to chloride. Compared to Co, SO played a more important role in the degradation of CXR-type OBPs and the conversion from chloride to final by-product chlorate. The adverse effects that results from Co need more attention in SO-based AOPs application.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124243DOI Listing
March 2021

Pattern recognition based on machine learning identifies oil adulteration and edible oil mixtures.

Nat Commun 2020 10 23;11(1):5353. Epub 2020 Oct 23.

Yihai Kerry Arawana Oils, Grains & Food Co., Ltd, Arawana Building, No. 1379 Bocheng Road, Pudong New District, Shanghai, China.

Previous studies have shown that each edible oil type has its own characteristic fatty acid profile; however, no method has yet been described allowing the identification of oil types simply based on this characteristic. Moreover, the fatty acid profile of a specific oil type can be mimicked by a mixture of 2 or more oil types. This has led to fraudulent oil adulteration and intentional mislabeling of edible oils threatening food safety and endangering public health. Here, we present a machine learning method to uncover fatty acid patterns discriminative for ten different plant oil types and their intra-variability. We also describe a supervised end-to-end learning method that can be generalized to oil composition of any given mixtures. Trained on a large number of simulated oil mixtures, independent test dataset validation demonstrates that the model has a 50 percentile absolute error between 1.4-1.8% and a 90 percentile error of 4-5.4% for any 3-way mixtures of the ten oil types. The deep learning model can also be further refined with on-line training. Because oil-producing plants have diverse geographical origins and hence slightly varying fatty acid profiles, an online-training method provides also a way to capture useful knowledge presently unavailable. Our method allows the ability to control product quality, determining the fair price of purchased oils and in-turn allowing health-conscious consumers the future of accurate labeling.
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http://dx.doi.org/10.1038/s41467-020-19137-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584611PMC
October 2020

Exome-Wide Association Analysis Suggests LRP2BP as a Susceptibility Gene for Endothelial Injury in Systemic Sclerosis in the Han Chinese Population.

J Invest Dermatol 2020 Oct 15. Epub 2020 Oct 15.

State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, Shanghai, China; Human Phenome Institute, Fudan University, Shanghai, China; Research Unit of dissecting the population genetics and developing new technologies for treatment and prevention of skin phenotypes and dermatological diseases (2019RU058), Chinese Academy of Medical Sciences, Beijing, China; Institute of Rheumatology, Immunology and Allergy, Fudan University, Shanghai, China. Electronic address:

Genetic factors play a key role in the pathogenesis of autoimmune diseases, whereas the disease-causing variants remain largely unknown. Herein, we performed an exome-wide association study of systemic sclerosis in a Han Chinese population. In the discovery stage, 527 patients with systemic sclerosis and 5,024 controls were recruited and genotyped. In the validation study, an independent sample set of 479 patients and 1,096 controls were examined. In total, we found that four independent signals reached genome-wide significance. Among them, rs7574865 (P = 3.87 × 10) located within signal transducer and activator of transcription 4 gene was identified previously using samples of European ancestry. Additionally, another signal including three SNPs in linkage disequilibrium might be unreported susceptibility loci located in the epidermis differentiation complex region. Furthermore, two SNPs located within exon 3 of IGHM (rs45471499, P = 1.15 × 10) and upstream of LRP2BP (rs4317244, P = 4.17 × 10) were found. Moreover, rs4317244 was identified as an expression quantitative trait locus for LRP2BP that regulates tight junctions, cell cycle, and apoptosis in endothelial cell lines. Collectively, our results revealed three signals associated with systemic sclerosis in Han Chinese and suggested the importance of LRP2BP in systemic sclerosis pathogenesis. Given the limited sample size and discrepancies between previous results and our study, further studies in multiethnic populations are required for verification.
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http://dx.doi.org/10.1016/j.jid.2020.07.039DOI Listing
October 2020

ACE2 Expression on the Keratinocytes and SARS-CoV-2 Percutaneous Transmission: Are they Related?

J Invest Dermatol 2021 01 15;141(1):197-198. Epub 2020 Oct 15.

Department of Dermatology, Hunan Key Laboratory of Medical Epigenetics, Second Xiangya Hospital, Central South University, Changsha, China. Electronic address:

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http://dx.doi.org/10.1016/j.jid.2020.09.019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7558226PMC
January 2021

[Chinese traditional cultural belief of medical students and its relationship with depression].

Nan Fang Yi Ke Da Xue Xue Bao 2020 Aug;40(8):1178-1183

Department of Psychology, School of Public Health, Southern Medical University, Guangzhou 510515, China.

To study the cultural beliefs of medical students and its relationship with depression.A questionnaire survey was conducted among 977 medical students using the Chinese Traditional Culture Belief Scale (CTCBS) and Nine Item-Patient Health Questionnaire (PHQ-9).The total score of medical students' cultural belief was 48.34± 7.31, and 54.4% of them had clear cultural belief. Cultural belief did not differ significantly among the medical students with different genders, places of residence or economic status, but those with ideals and beliefs had significantly higher cultural belief than those without (48.87±7.40 47.09±6.95, < 0.001). Cultural belief was negatively correlated to depression among the medical students (=-0.215, < 0.001), and those with a high-level cultural belief had lower levels of depression (6.09±3.88 7.42 ± 3.82 9.00 ± 4.90, < 0.001). The total and all the dimensions scores of CTCBS of the students without depressive symptoms were significantly higher than those in students with depressive symptoms. The medical students with lower cultural beliefs had a higher positive rate for depression screening (37.8% 18.8% 12.2%).Medical students have a high level of cultural beliefs. Strong culture beliefs are beneficial to relieve depression symptoms and reduce the incidence rate of depression. Chinese traditional culture education should be strengthened in the quality education of medical students.
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http://dx.doi.org/10.12122/j.issn.1673-4254.2020.08.16DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7429171PMC
August 2020

The aryl hydrocarbon receptor: An environmental effector in the pathogenesis of fibrosis.

Pharmacol Res 2020 10 30;160:105180. Epub 2020 Aug 30.

Department of Dermatology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China; Hunan Key Laboratory of Medical Epigenetics, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China. Electronic address:

The aryl hydrocarbon receptor (AhR) is a highly conserved transcription factor that can be activated by small molecules provided by dietary, plant, or microbial metabolites, and environmental pollutants. AhR is expressed in many cell types and engages in crosstalk with other signaling pathways, and therefore provides a molecular pathway that integrates environmental cues and metabolic processes. Fibrosis, which is defined as an aberrant extracellular matrix formation, is a reparative process in the terminal stage of chronic diseases. Both environmental and internal factors have been shown to participate in the pathogenesis of fibrosis; however, the underlying mechanisms still remain elusive. In this review, the potential role of AhR in the process of fibrosis, as well as potential opportunities and challenges in the development of AhR targeting therapeutics, are summarized.
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http://dx.doi.org/10.1016/j.phrs.2020.105180DOI Listing
October 2020

Parenting Style and Emotional Distress Among Chinese College Students: A Potential Mediating Role of the Zhongyong Thinking Style.

Front Psychol 2020 28;11:1774. Epub 2020 Jul 28.

Department of Psychology, School of Public Health, Southern Medical University, Guangzhou, China.

Previous studies suggested that parenting style was associated with college student's emotional distress. However, little is known about the underlying mechanisms of this relation in Chinese culture. The present study investigated the associations between parenting style and college student's emotional distress (depression and anxiety symptoms), examined the mediating effects of Confucian personality-Zhongyong thinking, and explored whether gender, age, and socioeconomic status (SES) moderated the direct and/or indirect effects of parenting style on emotional distress. Results from a large representative sample of Chinese college students ( = 3943) indicated that (a) parental rejection and overprotection was positively and mildly associated with depressive and anxiety symptoms and negatively and mildly related to Zhongyong thinking. Parental warmth significantly correlated with the three variables in the opposite direction; Zhongyong thinking correlated negatively and moderately with depression, and mildly with anxiety; (b) Zhongyong thinking partially mediated the associations of parental rejection and warmth with emotional distress. Specifically, to the extent that students perceived less rejection and more warmth, they were more likely to develop Zhongyong thinking associated with decreased emotional distress; (c) gender and SES moderated the association between parenting style and Zhongyong thinking. Specifically, for students with low SES, the negative relationship between parental overprotection and Zhongyong thinking was stronger; for males and high SES students, the positive link between parental warmth and Zhongyong thinking were stronger. Results highlight the importance of researching potential effects of college student's Zhongyong thinking within the family system in Chinese culture.
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http://dx.doi.org/10.3389/fpsyg.2020.01774DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7399746PMC
July 2020

Aryl hydrocarbon receptor signaling activation in systemic sclerosis attenuates collagen production and is a potential antifibrotic target.

Int Immunopharmacol 2020 Nov 12;88:106886. Epub 2020 Aug 12.

Department of Dermatology, Second Xiangya Hospital, Central South University, Changsha 410000, Hunan, China; Hunan Key Laboratory of Medical Epigenetics, Department of Dermatology, The Second Xiangya Hospital, Central South University, Changsha 410000, Hunan, China. Electronic address:

Systemic sclerosis (SSc) is a systemic autoimmune disease that often leads to fibrosis of multiple organs, and there are no effective treatments. Aryl hydrocarbon receptor (AhR) is a highly evolutionarily conserved transcription factor activated by endogenous and exogenous ligands and that regulate cell proliferation, tumorigenesis and immune balance. Recently, it have reported AhR signaling may participate in fibrosis process, usually consider as a negative regulator of TGF-β. However, the detailed relationship between AhR and SSc has not been reported yet. Here we firstly found that AhR and CYP1A1 downregulated in SSc fibroblast(n = 6). The AhR ligand-Ficz negatively regulates TGF-β1, COL1A1 and α-SMA expression, also enhances the MMP-1 expression via the AhR signaling activation. Conversely the AhR antagonist CH223191 could inhibit this effect. Furthermore, the antifibrosis effect of AhR signaling activation was also confirmed in bleomycin induced scleroderma mouse model. In conclusion, AhR signaling activation balances the extracellular matrix (ECM) composition and deposition, which may provide a new sight to the pathogenesis of SSc and AhR signaling activation may be a potential therapy for SSc.
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http://dx.doi.org/10.1016/j.intimp.2020.106886DOI Listing
November 2020

[Effects of resistance training on mitochondrial function in skeletal muscle of aging rats].

Zhongguo Ying Yong Sheng Li Xue Za Zhi 2020 Mar;36(2):165-170

College of Physical Education, Provincial Key Laboratory of Sports Human Science, Liaoning Normal University, Dalian 116029.

To investigate the effects of resistance exercise on mitochondrial function in skeletal muscle of aging rats. Forty male Sprague-Dawley rats were randomly assigned to 4 groups, 2-month sedentary control group (C1; =10), 2-month with resistance training group (R1; =10), 6-month sedentary control group (C2; =10), 6-month with resistance training group (R2; =10 ). Rats in R1 and R2 groups were arranged for resistance training for 8 weeks. This program consisted of interval running on a treadmill, speed 15 m·min, 35° incline, duration 15 s, interval 30 s, 4 times/group, 3 groups/cycle, 2 cycles per day, 6 days per week, a total of 8 weeks. The expressions of mitochondrial fusion protein 2(Mfn2) and dynamin-related protein 1(DRP1) in rat quadriceps were detected by Western blot, and the changes of mitochondrial membrane potential (ΔΨm), reactive oxygen species (ROS) and Ca concentration were measured by flow cytometry. ①Compared with C1 group, the expression of DRP1 protein in R1 group was increased (<0. 01), and the Mfn2 protein in R1 group had no significant difference, both DRP1 and Mfn2 protein in C2 group were decreased (<0. 01);compared with C2 group, the DRP1 and Mfn2 protein in R2 group were similarly increased (<0. 01, <0. 05);compared with R1 group, the DRP1 and Mfn2 protein in R2 group were both decreased (<0. 01). ② Compared with C1 group, the Ca content of R1 group was decreased (<0. 01) and the Ca content of C2 group was increased (<0. 01);Compared with C2 group, the content of Ca in R2 group was decreased (<0. 01);compared with R1 group, the Ca content in R2 group was increased (<0. 01). ③ Compared with C1 group, the ROS content in R1 group was increased, but there was no significant difference, while the ROS content in C2 group was increased (<0. 01);compared with C2 group, ROS content in R2 group was decreased (<0. 01); compared with R1 group, the ROS content in R2 group was increased (<0. 01). ④ Compared with group C1, the levels of ΔΨm in C2 group was decreased (<0. 01);Compared with C2 group, The ΔΨm of R2 group was increased(<0. 01); Compared with group R1, the ΔΨm of R2 group was decreased, but there was no statistical difference. During the aging process of rats, mitochondria of quadriceps femoral muscle showed Ca accumulation, increased reactive oxygen species, decreased mitochondrial membrane potential, decreased fusion protein and other phenomena, and resistance training could effectively improve these changes.
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http://dx.doi.org/10.12047/j.cjap.5861.2020.037DOI Listing
March 2020

Stronger network connectivity with lower diversity of soil fungal community was presented in coastal marshes after sixteen years of freshwater restoration.

Sci Total Environ 2020 Nov 5;744:140623. Epub 2020 Jul 5.

School of Plant, Environmental and Soil Sciences, Louisiana State Univ. Agricultural Center, Baton Rouge, LA 70803, USA.

Freshwater input for salt marsh restoration in the Yellow River Delta induced Phragmites australis expansion and thus may cause shifts of soil fungi from halophilic to desalination-adapted species for increased litter decomposition. In this study, soil fungal communities of restored and natural salt marshes were determined to reveal further details of shift in soil fungal community and its probable prediction for salt marsh restoration. Our results showed a stronger network within Ascomycota (e.g. Sordariales, Aspergillus, Hypocreales and Cladosporium herbarum) in restored marshes, but with a lower diversity of halophilic taxa (e.g. Chytridiomycota and Nematoda) in comparison with natural salt marshes. Contrarily, the occurrence of Chytridiomycota, Ichthyosporea and Discicristoidea in the soil fungal networks of the natural salt marsh emphasized the importance of salt tolerant species at the land-sea transition zone. The Sordariales was dominant and had a strong correlation with other fungal species and aggregate associated soil organic carbon (SOC), which probably contributed to SOC accumulation in restored marshes. But the reduced halophilic species specific to salt marsh elucidated that the formation of monospecific stands of P. australis along with the freshwater input induced desalination to the saline habitats changed the native patterns of vegetation and soil organisms. As the buffer between terrestrial and marine systems, a single habitat type such as dense monocultures of P. australis must be avoided and diverse saltmarsh habitats across a salinity gradient should be reserved. In this way, the diversity and specificity of coastal halophytes and related microorganisms could be maintained and thus might confer benefits in balancing various functions of the salt marsh ecosystem and preserving the system's elasticity and resistance to stress.
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http://dx.doi.org/10.1016/j.scitotenv.2020.140623DOI Listing
November 2020

Investigation for the transcultural self-efficacy of nurses in Guizhou, China.

Int J Nurs Sci 2020 Apr 19;7(2):191-197. Epub 2020 Mar 19.

Chongqing Population and Family Planning Science and Technology Research Institute, Chongqing, China.

Aim: As conflict caused by cultural diversity among patients in China continues to rise, hospitals are in urgent need for improvement of transcultural efficacy among nurses. This study aims to evaluate the transcultural self-efficacy of nurses working in the tertiary general hospital in Guizhou Province, an ethnic minority region in western China, and to identify whether nurses' demographic characteristics affect their transcultural self-efficacy.

Method: We used the Chinese version of the Transcultural Self-Efficacy Tool (TSET-CV) to survey 1,190 in-service nurses.

Results: Results showed that the level of transcultural self-efficacy of the nurses was generally moderate; few of the nurses had high or low transcultural self-efficacy. The nurses' transcultural self-efficacy was affected by demographic variables, including age, marital status, employment type, income, work experience, and whether or not they were head nurses. Having a stable work environment, a stable marriage, a good educational background, and a high-ranked professional title were associated with increased transcultural self-efficacy.

Conclusion: Nursing administrators in hospitals should offer continuing education on transcultural nursing according to nurses' demographic characteristics and the SEST scores.
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http://dx.doi.org/10.1016/j.ijnss.2020.03.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355191PMC
April 2020

27-Hydroxycholesterol contributes to cognitive deficits in APP/PS1 transgenic mice through microbiota dysbiosis and intestinal barrier dysfunction.

J Neuroinflammation 2020 Jun 27;17(1):199. Epub 2020 Jun 27.

School of Public Health, Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, No.10 Xitoutiao, You An Men Wai, Beijing, 100069, China.

Background: Research on the brain-gut-microbiota axis has led to accumulating interest in gut microbiota dysbiosis and intestinal barrier dysfunction in Alzheimer's disease (AD). Our previous studies have demonstrated neurotoxic effects of 27-hydroxycholesterol (27-OHC) in in vitro and in vivo models. Here, alterations in the gut microbiota and intestinal barrier functions were investigated as the possible causes of cognitive deficits induced by 27-OHC treatment.

Methods: Male APP/PS1 transgenic and C57BL/6J mice were treated for 3 weeks with 27-OHC (5.5 mg/kg/day, subcutaneous injection) and either a 27-OHC synthetase inhibitor (anastrozole, ANS) or saline. The Morris water maze and passive avoidance test were used to assess cognitive impairment. Injuries of the intestine were evaluated by histopathological examination. Intestinal barrier function was assessed by plasma diamine oxidase (DAO) activity and D-lactate. Systemic and intestinal inflammation were evaluated by IL-1β, TNF-α, IL-10, and IL-17 concentrations as determined by ELISA. The fecal microbiome and short-chain fatty acids (SCFAs) were analyzed using 16S rDNA sequencing and ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). Tight junction proteins were evaluated in the ileum and colon by qRT-PCR and Western blots. Tight junction ultrastructure was examined by transmission electron microscopy.

Results: Treatment with 27-OHC resulted in severe pathologies in the ileum and colon. There was impaired intestinal barrier integrity as indicated by dilated tight junctions and downregulation of tight junction proteins, including occludin, claudin 1, claudin 5, and ZO-1, and signs of inflammation (increased IL-1β, TNF-α, and IL-17). Fecal 16S rDNA sequencing and taxonomic analysis further revealed a decreased abundance of Roseburia and reduced fecal levels of several SCFAs in 27-OHC-treated mice. Meanwhile, co-treatment with ANS reduced intestinal inflammation and partially preserved intestinal barrier integrity in the presence of 27-OHC.

Conclusions: The current study demonstrates for the first time that 27-OHC treatment aggravates AD-associated pathophysiological alterations, specifically gut microbiota dysbiosis and intestinal barrier dysfunction, which suggests that the gut microbiome and intestinal barrier function warrant further investigation as potential targets to mitigate the neurotoxic impact of 27-OHC on cognitive function and the development of AD.
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http://dx.doi.org/10.1186/s12974-020-01873-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321549PMC
June 2020

A deep learning, image based approach for automated diagnosis for inflammatory skin diseases.

Ann Transl Med 2020 May;8(9):581

Department of Dermatology, Second Xiangya Hospital, Central South University, Hunan Key Laboratory of Medical Epigenomics, Changsha 410011, China.

Background: As the booming of deep learning era, especially the advances in convolutional neural networks (CNNs), CNNs have been applied in medicine fields like radiology and pathology. However, the application of CNNs in dermatology, which is also based on images, is very limited. Inflammatory skin diseases, such as psoriasis (Pso), eczema (Ecz), and atopic dermatitis (AD), are very easily to be mis-diagnosed in practice.

Methods: Based on the EfficientNet-b4 CNN algorithm, we developed an artificial intelligence dermatology diagnosis assistant (AIDDA) for Pso, Ecz & AD and healthy skins (HC). The proposed CNN model was trained based on 4,740 clinical images, and the performance was evaluated on experts-confirmed clinical images grouped into 3 different dermatologist-labelled diagnosis classifications (HC, Pso, Ecz & AD).

Results: The overall diagnosis accuracy of AIDDA is 95.80%±0.09%, with the sensitivity of 94.40%±0.12% and specificity 97.20%±0.06%. AIDDA showed accuracy for Pso is 89.46%, with sensitivity of 91.4% and specificity of 95.48%, and accuracy for AD & Ecz 92.57%, with sensitivity of 94.56% and specificity of 94.41%.

Conclusions: AIDDA is thus already achieving an impact in the diagnosis of inflammatory skin diseases, highlighting how deep learning network tools can help advance clinical practice.
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http://dx.doi.org/10.21037/atm.2020.04.39DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290553PMC
May 2020

DDeep3M: Docker-powered deep learning for biomedical image segmentation.

J Neurosci Methods 2020 08 18;342:108804. Epub 2020 Jun 18.

Sino-German Tongji-Caritas Research Center of Ultrasound in Medicine, Department of Medical Ultrasound, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, China.

Background: Deep learning models are turning out to be increasingly popular in biomedical image processing. The fruitful utilization of these models, in most cases, is substantially restricted by the complicated configuration of computational environments, resulting in the noteworthy increment of the time and endeavors to reproduce the outcomes of the models.

New Method: We thus present a Docker-based method for better use of deep learning models and quicker reproduction of model performance for multiple data sources, permitting progressively more biomedical scientists to attempt the new technology conveniently in their domain. Here, we introduce a Docker-powered deep learning model, named as DDeep3M and validated it with the electron microscopy data volumes (microscale).

Results: DDeep3M is utilized to the 3D optical microscopy image stack in mouse brain for the image segmentation (mesoscale). It achieves high accuracy on both vessels and somata structures with all the recall/precision scores and Dice indexes over 0.96. DDeep3M also reports the state-of-the-art performance in the MRI data (macroscale) for brain tumor segmentation.

Comparison With Existing Methods: We compare the performance and efficiency of DDeep3M with three existing models on image datasets varying from micro- to macro-scales.

Conclusion: DDeep3M is a friendly, convenient and efficient tool for image segmentations in biomedical research. DDeep3M is open sourced with the codes and pretrained model weights available at https://github.com/cakuba/DDeep3m.
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http://dx.doi.org/10.1016/j.jneumeth.2020.108804DOI Listing
August 2020

Potential role of the skin microbiota in Inflammatory skin diseases.

J Cosmet Dermatol 2021 Feb 20;20(2):400-409. Epub 2020 Jun 20.

Department of Dermatology, Second Xiangya Hospital, Central South University, Changsha, China.

Background: Inflammatory skin diseases include a variety of skin diseases, such as seborrheic dermatitis, acne, atopic dermatitis, psoriasis and so on, which are more common and tend to have a significant impact on patients' quality of life. Inflammatory skin diseases often result in physical or psychological distress; however, the pathogenesis of these diseases have not been clearly elucidated. Many factors are involved in the pathogenesis of inflammatory skin diseases, including heredity, environment, immunity, epidermal barrier, mental disorders, infection and so on. In recent years, skin microbiota has been shown to play an important role in inflammatory skin diseases.

Aims: To elaborate on the specific mechanisms of inflammatory skin diseases induced by microbiota dysbiosis.

Methods: We introduce the function and influence of skin microbiota in inflammatory skin diseases from the following aspects: Immunity, epigenetics, epidermal barrier and treatment.

Results: Skin microbiota can affect many aspects of the host, such as Immunity, epigenetics, epidermal barrier, and it plays an important role in the pathogenesis of inflammatory skin diseases.

Conclusion: Skin microbiota is extremely important for maintaining the health of skin and the dysbiosis of skin microbiota is an important pathogenesis of inflammatory skin diseases.
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http://dx.doi.org/10.1111/jocd.13538DOI Listing
February 2021

Spätzle Homolog-Mediated Toll-Like Pathway Regulates Innate Immune Responses to Maintain the Homeostasis of Gut Microbiota in the Red Palm Weevil, Olivier (Coleoptera: Dryophthoridae).

Front Microbiol 2020 25;11:846. Epub 2020 May 25.

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China.

Spätzle (Spz) is a dimeric ligand that responds to the Gram-positive bacterial or fungal infection by binding Toll receptors to induce the secretion of antimicrobial peptides. However, whether the Toll-like signaling pathway mediates the innate immunity of to modulate the homeostasis of gut microbiota has not been determined. In this study, we found that a Spz homolog, RfSpätzle, is a secretory protein comprising a signal peptide and a conservative Spz domain. RT-qPCR analysis revealed that was significantly induced to be expressed in the fat body and gut by the systemic and oral infection with pathogenic microbes. The expression levels of two antimicrobial peptide genes, and , were downregulated significantly by knockdown, indicating that their secretion is under the regulation of the -mediated signaling pathway. After being challenged by pathogenic microbes, the cumulative mortality rate of -silenced individuals was drastically increased as compared to that of the controls. Further analysis indicated that these larvae possessed the diminished antibacterial activity. Moreover, knockdown altered the relative abundance of gut bacteria at the phylum and family levels. Taken together, these findings suggest that is involved in RPW immunity to confer protection and maintain the homeostasis of gut microbiota by mediating the production of antimicrobial peptides.
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http://dx.doi.org/10.3389/fmicb.2020.00846DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261851PMC
May 2020

Successful management of giant condyloma acuminatum of vulva with the combination of surgery and photodynamic therapy: Report of two cases.

Photodiagnosis Photodyn Ther 2020 Sep 30;31:101847. Epub 2020 May 30.

Department of Dermatology, The Second Xiangya Hospital of Central South University, Changsha 410011, China. Electronic address:

Patients with condylomata acuminata of the vulva usually have increased difficulty to achieve complete response to treatment and also have a higher risk for disease recurrence. Treatment for this disease varies, including surgical excision, cryotherapy, electrocautery, CO2 laser therapy, topical therapy, and photodynamic therapy (PDT), but none of these alone provides a satisfactory outcome, especially for giant condyloma acuminatum (GCA). We reported two cases of GCA successfully cured with surgical removal, electrocautery and photodynamic therapy.
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http://dx.doi.org/10.1016/j.pdpdt.2020.101847DOI Listing
September 2020

-fatty acids alter the gut microbiota in high-fat-diet-induced obese rats.

Br J Nutr 2020 Dec 1;124(12):1251-1263. Epub 2020 Jun 1.

School of Public Health, Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing100069, People's Republic of China.

The gut microbiota is directly influenced by dietary components, and it plays critical roles in chronic diseases. Excessive consumption of trans-fatty acids (TFA) is associated with obesity induced by alterations in gut microbiota, but the links between obesity and gut microbiota remain unclear. Therefore, studies examining the impact of TFA on intestinal microflora are essential. In our study, we performed 16S ribosomal RNA gene sequencing on faecal samples from Sprague-Dawley rats fed a basal diet (control (CON) group), high-fat (HF) diet (diet-induced obesity (DIO) group) or TFA diets (1 % TFA group and 8 % TFA group) for 8 weeks to investigate the effects of TFA/HF diets on obesity and gut microbiota composition. We found that the TFA/HF diets significantly induced obesity and changes in blood and brain physiological parameters of the rats. The relative abundance of the phyla Firmicutes and Bacteroidetes was inversely altered in the three test groups compared with the CON group. Proteobacteria increased slightly in the DIO, 1 % TFA and 8 % TFA groups. The genus Bacteroides increased in the DIO and 1 % TFA groups, but Muribaculaceae decreased in all experimental groups compared with the CON group. Moreover, significant differences were observed among clusters of orthologous group functional categories of the four dietary groups. Our observations suggested that the TFA/HF diets induced obesity and dysfunction of gut microbiota. Gut dysbiosis might mediate the obesity effects of TFA/HF diets.
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http://dx.doi.org/10.1017/S0007114520001841DOI Listing
December 2020