Publications by authors named "Qingqing Hao"

17 Publications

  • Page 1 of 1

Serum NSE and S100B protein levels for evaluating the impaired consciousness in patients with acute carbon monoxide poisoning.

Medicine (Baltimore) 2021 Jun;100(25):e26458

Department of Emergency.

Abstract: The aim of this study was to investigate the associations between the levels of neuron-specific enolase (NSE) and S100B protein and coma duration, and evaluate the optimal cut-off values for prediction coma duration ≥ 72 hours in patients with acute carbon monoxide poisoning (ACOP).A total of 60 patients with ACOP were divided into 3 following groups according to their status of consciousness and coma duration at admission: Awake group [Glasgow Coma Scale score (GCS score) ≥ 13 points], Coma < 72 hours group (GCS score < 13 points and coma duration < 72 h), and Coma ≥ 72 hours group (GCS score < 13 points and coma duration ≥ 72 h). The levels of serum NSE and S100B protein were measured after admission.There were significant differences in GCS score, carbon monoxide (CO) exposure time, NSE, and S100B levels between the Coma ≥ 72 h group and the Awake group, and between the Coma < 72 h group and the Awake group. Significant differences in GCS score, NSE, and S100B levels were also found between Coma ≥ 72 h group and Coma < 72 h group. Correlation analysis showed that NSE and S100B were positively correlated (rs = 0.590, P < .01); NSE and S100B were negatively correlated with GCS score (rs = -0.583, rs = -0.590, respectively, both P < .01). The areas under the curve (AUCs) of NSE, S100B, and GCS score to predict the coma duration ≥ 72 hours were 0.754, 0.791, and 0.785, respectively. Pairwise comparisons did not show differences among the 3 groups (all P > .05). The sensitivity and specificity of NSE prediction with a cut-off value of 13 μg/L were 80% and 64%, respectively, and those of S100B prediction with a cut-off value of 0.43 μg/L were 70% and 88%, respectively.The NSE and S100B protein levels were significantly correlated with the degree of impaired consciousness and had the same clinical value in predicting coma duration of ≥ 72 hours in patients with ACOP.
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http://dx.doi.org/10.1097/MD.0000000000026458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8238324PMC
June 2021

Transcriptome-Wide Identification of WRKY Transcription Factors and Their Expression Profiles under Different Types of Biological and Abiotic Stress in  Lamb.

Genes (Basel) 2020 11 23;11(11). Epub 2020 Nov 23.

Key Laboratory of Forestry Genetics & Biotechnology of Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.

Lamb, an economically important conifer tree, is widely distributed in China. WRKY transcription factors (TFs) play important roles in plant growth and development, biological and abiotic stress. Nevertheless, there is little information about the WRKY genes in . By searching for conserved WRKY motifs in transcriptomic RNA sequencing data for , 31 sequences were identified as WRKY TFs. Then, phylogenetic and conserved motif analyses of the WRKY family in , and were used to classify WRKY genes. The expression patterns of six genes from different groups were determined using real-time quantitative PCR for 2-year-old seedings grown in their natural environment and challenged by phytohormones (salicylic acid, methyl jasmonate, or ethephon), abiotic stress (HO) and mechanical damage stress. As a result, the 31 genes identified were divided into three major groups and several subgroups based on structural and phylogenetic features. genes are regulated in response to abiotic stress and phytohormone treatment and may participate in signaling to improve plant stress resistance. Some genes behaved as predicted based on their homology with WRKY genes, but others showed divergent behavior. This systematic analysis lays the foundation for further identification of WRKY gene functions to aid further exploration of the functions and regulatory mechanisms of genes in biological and abiotic stress in .
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http://dx.doi.org/10.3390/genes11111386DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7700256PMC
November 2020

Cardiac contractility modulation attenuates structural and electrical remodeling in a chronic heart failure rabbit model.

J Int Med Res 2020 Oct;48(10):300060520962910

Department of Cardiology, Hebei General Hospital, Shijiazhuang, Hebei Province, People's Republic of China.

Background: Cardiac contractility modulation (CCM) is non-excitatory electrical stimulation for improving cardiac function. This study aimed to evaluate the effects of CCM on structural and electrical remodeling in a rabbit model of chronic heart failure (CHF).

Methods: Thirty rabbits were randomly divided into the sham, CHF, and CCM groups. The CHF model was induced 12 weeks after trans-aortic constriction by pressure unloading and CCM was delivered to the myocardium for 4 weeks. Corrected QT intervals, the ventricular effective refractory period, and inducibility of ventricular tachycardia were measured by an electrophysiological examination. Connective tissue growth factor, galectin-3, Kv4.3, KCNQ1, KCNH2, and connexin 43 protein levels were measured by western blotting.

Results: The CHF group had a significantly prolonged corrected QT interval and ventricular effective refractory period, and increased inducibility of ventricular tachycardia. Prominent myocardial fibrosis and increased hydroxyproline content were observed in the CHF group, but these were suppressed in the CCM group. Kv4.3, KCNQ1, KCNH2, and connexin 43 protein levels were significantly lower in the CHF group, but treatment with CCM partially restored their levels.

Conclusions: CCM attenuates myocardial structural and electrical remodeling during CHF. These findings provide evidence for clinical use of CCM in treating CHF.
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http://dx.doi.org/10.1177/0300060520962910DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556184PMC
October 2020

Chondroma in the Area of the Spheno-Ethmoid Junction.

J Craniofac Surg 2020 Oct;31(7):e720-e722

Department of Otorhinolaryngology Head and Neck Surgery, Beijing Chaoyang Hospital, Capital Medical University; Beijing, China.

Chondroma in the area of the spheno-ethmoidal junction is very rare. A 29-year-old male patient with chronic rhinosinusitis with nasal polyps was arranged for a preoperative computed tomography scan, and a lesion was accidentally found in his spheno-ethmoidal junction and involved the skull base. Combined with MRI, the lesion was misdiagnosed as fungal sinusitis. However, no fungal lesions were found during the operation, and cartilage tissue was confirmed only after some bone was ground away under the guidance of a surgical navigation system. Our case indicates that chondroma is easily misdiagnosed as fungal sinusitis when it appears in the sinuses and should be carefully distinguished from fungal sinusitis. Moreover, when lesions involve the skull base, surgical navigation systems are useful in accurately locating lesions.
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http://dx.doi.org/10.1097/SCS.0000000000006686DOI Listing
October 2020

Cardiac Contractility Modulation Attenuates Chronic Heart Failure in a Rabbit Model via the PI3K/AKT Pathway.

Biomed Res Int 2020 7;2020:1625362. Epub 2020 Jan 7.

School of Graduate, Hebei Medical University, Shijiazhuang, China.

The Akt plays an important role in regulating cardiac growth, myocardial angiogenesis, and cell death in cardiac myocytes. However, there are few studies to focus on the responses of the Akt pathway to cardiac contractility modulation (CCM) in a chronic heart failure (HF) model. In this study, the effects of CCM on the treatment of HF in a rabbit model were investigated. Thirty six-month-old rabbits were randomly separated into control, HF, and CCM groups. The rabbits in HF and CCM groups were pressure uploaded, which can cause an aortic constriction. Then, CCM was gradually injected to the myocardium of rabbits in the CCM group, and this process lasted for four weeks with six hours per day. Rabbit body weight, heart weight, and heart beating rates were recorded during the experiment. To assess the CCM impacts, rabbit myocardial histology was examined as well. Additionally, western blot analysis was employed to measure the protein levels of Akt, FOXO3, Beclin, Pi3k, mTOR, GSK-3, and TORC2 in the myocardial histology of rabbits. Results showed that the body and heart weight of rabbits decreased significantly after suffering HF when compared with those in the control group. However, they gradually recovered after CCM application. The CCM significantly decreased collagen volume fraction in myocardial histology of HF rabbits, indicating that CCM therapy attenuated myocardial fibrosis and collagen deposition. The levels of Akt, FOXO3, Beclin, mTOR, GSK-3, and TORC2 were significantly downregulated, but Pi3k concentration was greatly upregulated after CCM utilization. Based on these findings, it was concluded that CCM could elicit positive effects on HF therapy, which was potentially due to the variation in the Pi3k/Akt signaling pathway.
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http://dx.doi.org/10.1155/2020/1625362DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6973194PMC
September 2020

Synergistic conversion of coal char and methane for syngas and carbon-based supercapacitor electrodes.

J Colloid Interface Sci 2020 Mar 7;562:235-243. Epub 2019 Dec 7.

School of Chemical Engineering, Northwest University, Xi'an 710069, China; Chemical Engineering Research Center of the Ministry of Education for Advanced Use Technology of Shanbei Energy, Shaanxi Research Center of Engineering Technology for Clean Coal Conversion, Northwest University, Xi'an 710069, China; International Science & Technology Cooperation Base of MOST for Clean Utilization of Hydrocarbon Resources, Collaborative Innovation Center for Development of Energy and Chemical Industry in Northern Shaanxi, Xi'an 710069, China.

A facile one-pot process for synergistic conversion of coal char and methane is conducted by employing KCO as the catalyst. Besides syngas production, valuable carbon products are obtained and used to serve for supercapacitor electrodes. Effect of the operating parameters (including the catalyst dosage, gas feed flow rate, reaction temperature and time) is evaluated on electrochemical performance of the as-prepared carbon. The appropriate surface and structural properties enable the prepared carbon electrode to have a remarkable capacitive performance, along with a specific capacitance up to 125 F/g at a scan rate of 5 mV/s and 133 F/g at a current density of 1 A/g. According to the potential capacitive contribution of the carbon species, the high capacitive performance is mainly attributed to formation and growth of abundant carbon fibers in the one-pot process.
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http://dx.doi.org/10.1016/j.jcis.2019.12.024DOI Listing
March 2020

miRNA-103 promotes chondrocyte apoptosis by down-regulation of Sphingosine kinase-1 and ameliorates PI3K/AKT pathway in osteoarthritis.

Biosci Rep 2019 10;39(10)

Department of Rheumatism and Immunology, Hebei General Hospital, Shijiazhuang City 050051, Heibei Province, P.R. China.

Objectives: The aim of the present study was to determine the effects of miRNA-103 on chondrocyte apoptosis and molecular mechanisms in osteoarthritis (OA) progression.

Methods: The cell proliferation, apoptosis, and recovery ability were measured by cell counting kit-8 (CCK-8), flow cytometry, and wound healing assays. The interaction of miRNA-103 and Sphingosine kinase-1 (SPHK1) were determined by using luciferase reporter assay. The expression of mRNA and proteins were measured by qRT-PCR and Western blot. OA rat model was established by surgery stimulation.

Results: miRNA-103 expression was significantly increased in the cartilage of OA patients and surgery-induced OA rat models. miRNA-103 transfection into primary rat chondrocytes reduced SPHK1 expression, induced apoptosis, inhibited cell proliferation, and impeded scratch assay wound closure. Moreover, expression of total AKT, and p-AKT were significantly reduced in miRNA-103-overexpressing chondrocytes while SPHK1 up-regulation increased the expression of phosphatidylinsitol-3-kinase (PI3K) and p-AKT, and reversed the proliferation suppression induced by the miRNA-103 mimic.

Conclusions: Our studies suggest that miRNA-103 contributes to chondrocyte apoptosis, promoting OA progression by down-regulation of PI3K/AKT pathway through the reduction in SPHK1 activity.
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http://dx.doi.org/10.1042/BSR20191255DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6822578PMC
October 2019

Inhibition of SETD7 protects cardiomyocytes against hypoxia/reoxygenation-induced injury through regulating Keap1/Nrf2 signaling.

Biomed Pharmacother 2018 Oct 11;106:842-849. Epub 2018 Jul 11.

Department of Cardiology, Hebei General Hospital, No.348 West Heping Road, Shijiazhuang, Hebei 050051, China. Electronic address:

The protein SET domain-containing lysine methyltransferase 7 (SETD7) has recently been shown to regulate apoptosis in various cells. However, the role of SETD7 on cardiomyocyte apoptosis during myocardial ischemia/reperfusion injury remains unclear. This study aimed to investigate the potential role of SETD7 in hypoxia/reoxygenation (H/R)-induced apoptosis of rat cardiomyocytes and reveal the underlying mechanism. Our results demonstrated that SETD7 expression was significantly up-regulated in cardiomyocytes in response to H/R injury. The inhibition of SETD7 by siRNA-mediated gene silencing significantly suppressed H/R-induced apoptosis and decreased the production of reactive oxygen species (ROS). The overexpression of SETD7 markedly enhanced H/R-induced apoptosis and ROS production. Moreover, the knockdown of SETD7 reduced the expression of Keap1 and promoted the expression of Nrf2. In addition, the knockdown of SETD7 increased the activity of antioxidant response element and promoted the expression of heme oxygenase-1 and NADPH-quinone oxidoreductase 1. However, the knockdown of Nrf2 partially abrogated the SETD7 inhibition-mediated protective effect against H/R injury. Taken together, these results indicate that the inhibition of SETD7 attenuates H/R-induced injury of cardiomyocytes via the down-regulation of Keap1 and promotion of the Nrf2-mediated anti-oxidation signaling pathway.
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http://dx.doi.org/10.1016/j.biopha.2018.07.007DOI Listing
October 2018

Effects of Dual-Dose Clopidogrel, Clopidogrel Combined with Tongxinluo Capsule, and Ticagrelor on Patients with Coronary Heart Disease and CYP2C19*2 Gene Mutation After Percutaneous Coronary Interventions (PCI).

Med Sci Monit 2017 Aug 7;23:3824-3830. Epub 2017 Aug 7.

Department of Heart Center, Hebei General Hospital, Shijiazhuang, Hebei, China (mainland).

BACKGROUND In recent years, genetic factors have attracted research interest as important predisposing factors for cardiovascular susceptibility. This study aimed to investigate the influences of dual-dose clopidogrel, clopidogrel combined with tongxinluo, and ticagrelor on the platelet activity and MACE events of patients with CYP2C19*2 gene function deficiency and poor clopidogrel response after PCI. MATERIAL AND METHODS We selected 458 patients with coronary heart disease undergoing PCI, and the genotype of CYP2C19*2 was detected by TaqMan real-time PCR. We finally enrolled 212 patients and divided them into 4 groups: a standard anti-platelet group of 46 patients, a clopidogrel double-dose group of 50 cases, a clopidogrel combined with tongxinluo group of 59 cases, and a ticagrelor group of 57. The platelet inhibition rate was detected by TEG. We analyzed and compared differences in platelet activity and the occurrence of MACE events in these 4 groups at different follow-up times. RESULTS The results showed that inhibition of platelet aggregation was better in the double-dose clopidogrel group, the clopidogrel combined with tongxinluo group, and the ticagrelor group than in the regular-dose clopidogrel group, and ticagrelor was the best. We also found that the total incidence of MACE was much lower in the double-dose clopidogrel group, the clopidogrel combined with tongxinluo group, and the ticagrelor group, while the incidence of hemorrhage in the ticagrelor group was higher. CONCLUSIONS Adjusting the dose or combining with other drugs improves the efficacy of anti-platelet therapy and reduces the incidence of ischemic events after PCI.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5555704PMC
http://dx.doi.org/10.12659/msm.903054DOI Listing
August 2017

Angiotensin-Converting Enzyme 2 Inhibits Angiotensin II-Induced Abdominal Aortic Aneurysms in Mice.

Hum Gene Ther 2018 12 13;29(12):1387-1395. Epub 2018 Nov 13.

Department of Cardiology, Shandong Provincial Hospital Affiliated to Shandong University, Shandong, P.R. China.

Recent studies have demonstrated that angiotensin-converting enzyme 2 (ACE2) plays an important role in the pathogenesis of abdominal aortic aneurysms (AAAs). However, few studies have reported the direct effect of ACE2 overexpression on the aneurysm. This study hypothesized that the overexpression of ACE2 may prevent the pathogenesis of aneurysms by decreasing RAS activation. Thirty-nine mice were randomly assigned to three groups ( = 13 in each group): the Ad.ACE2 group, the group, and a control group. After 8 weeks of treatment, abdominal aortas with AAAs were obtained for hematoxylin and eosin staining, Verhoeff Van Gieson staining, immunohistochemistry, and Western blotting. The incidence and severity of AAAs, macrophage infiltration, and MMP protein expression were all recorded. The results showed that ACE2 gene transfer significantly decreased the occurrence of AAAs and inhibited AAA formation in mice by inhibiting the inflammatory response and MMP activation, and the mechanisms may involve decreased ERK and Ang II-nuclear factor kappa B signaling pathways.
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http://dx.doi.org/10.1089/hum.2016.144DOI Listing
December 2018

Preliminary application of intra-operative hearing monitoring by tone pip ABR via loudspeakers.

Acta Otolaryngol 2017 Feb 6;137(2):167-173. Epub 2016 Sep 6.

a Department of Otolaryngology/Head and Neck Surgery, Chinese PLA Institute of Otolaryngology , Chinese PLA General Hospital , Beijing , PR China.

Conclusion: This method might be an effective intra-operative way to access hearing threshold under general anesthesia in the operating room.

Objective: To develop an intra-operative hearing monitoring method for surgeons to access hearing threshold under general anesthesia in the operating room.

Method: System establishment and calibration was done firstly. Normal hearing (NH) and conductive hearing loss (CHL) participants were recruited. This study applied pure tone audiometry (PTA) to all subjects and tone pip ABR (tp-ABR) via loudspeakers in sound booth and operating room for NH and CHL subjects, respectively. For NH subjects, Bland Altman was conducted to compare 1k Hz PTA and tp-ABR threshold. For CHL participants, Paired t-test, Satterth waite t'-test, and linear correlation analysis were used to compare the two methods. p < .05 was considered statistically different.

Results: (1) Bland Altman showed all plots were inside the 95% confidence interval, indicating that there was no difference between the two methods for NH subjects. (2) The two methods had a high correlation (Pearson's coefficient =0.872 > 0.6, p < .05) in assessing hearing threshold in the operating room. (3) Satterth waite t'-test showed the ossicular chain malfunction enlarged the deviation of threshold by the two methods (.01 < p < .025).
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http://dx.doi.org/10.1080/00016489.2016.1218049DOI Listing
February 2017

Cardiac Contractility Modulation Attenuate Myocardial Fibrosis by Inhibiting TGF-β1/Smad3 Signaling Pathway in a Rabbit Model of Chronic Heart Failure.

Cell Physiol Biochem 2016 27;39(1):294-302. Epub 2016 Jun 27.

School of Graduate, Hebei Medical University, Shijiazhuang, China.

Unlabelled: Backgroun/Aims: To explore the effect of cardiac contractility modulation (CCM) on myocardial fibrosis in heart failure and to investigate the underlying mechanism.

Methods: Rabbits were randomly divided into sham group, HF group and CCM group. A rabbit model of chronic heart failure (CHF) was induced 12 weeks after aortic constriction by pressure unloading. Then cardiac contractility modulation was delivered to the myocardium lasting six hours per day for 4 weeks. Histology examination was carried out to evaluate the myocardial pathological changes. Protein levels of collagen I, collagen III, α-SMA, MMP2, MMP9, TIMP1, TGF-β1 and Smad3 were measured by western blot analysis.

Results: Histology examination results showed that CCM therapy attenuated myocardial fibrosis and collagen deposition in rabbits with CHF. In addition, protein levels of collagen I, collagen III, α-SMA, MMP2, MMP9, TIMP1, TGF-β1 and Smad3 were down regulated.

Conclusion: CCM therapy exerted protective effects against myocardial fibrosis potentially by inhibiting TGF-β1/Smad3 signaling pathway in CHF rabbits.
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http://dx.doi.org/10.1159/000445624DOI Listing
February 2017

[Analysis on the clinical outcomes of idiopathic sudden sensorineural hearing loss using the EuroQol 5-Dimension 3-Level questionnaire].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2016 Jan;30(2):126-9

Objective: To investigate the applicability of EuroQol 5-Dimension 3-Level questionnaire (Chinese Version 1.0) (EQ-5D-3L) in the assessment of life quality of patients with idopathic sudden hearing loss.

Method: In this retrospect study, the EQ-5D-3L was assigned to 60 patients with idopathic sudden hearing loss before and after drug therapy. Audiometry examinations were undertaken and concomitant symptoms were recorded. A Wilcoxon rank sum test was applicated to the comparisons of results of EQ-5D-3L before and after the treatment, a t-test for results of EQ-VAS and a Kappa measurement for the consistency of certain dimension correlated with the symptoms.

Result: Sixty patients completed the five questions and visual analog scale of the EQ-5D. A statistically significant changes of pain/discomfort, anxiety/depression before and after the treatment were observed (P < 0.05). The domains of mobility, self-care and usual activities did not show statistically significant different (P > 0.05). The dimension of pain/discomfort was in agreement with the concomitant symptoms. And the result of EQ-VAS was in agreement with the therapeutic outcome.

Conclusion: It is suitable for the EQ-5D to be a general health measure with sensitivity to clinical change in idiopathic sudden sensorineural hearing loss, which monitors the patients' outcomes.
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January 2016

Clinical analysis on surgical management of type III external auditory canal cholesteatoma: a report of 12 cases.

Acta Otolaryngol 2016 Oct 27;136(10):1006-10. Epub 2016 Apr 27.

a Department of Otolaryngology Head and Neck Surgery , Chinese PLA General Hospital , Beijing , PR China ;

Objective: To investigate surgical management of type III EACC with lesions involving the posterior external auditory canal and mastoid.

Methods: This retrospective case review was conducted in 12 patients with type III EACC who underwent intact canal wall mastoidectomy with tympanoplasty and canal wall reconstruction with autologous cortical bone.

Results: During the follow-up, all patients obtained successful results on external auditory canal structures and hearing improvement, except for one patient who needed a revision operation for the fistula between the reconstructed wall and the mastoid.

Conclusion: Intact canal wall mastoidectomy with tympanoplasty and canal wall reconstruction with autologous cortical bone was proved to be an effective and inexpensive choice for the patients with type III external auditory canal cholesteatoma (EACC) to achieve optimal outcomes.
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http://dx.doi.org/10.3109/00016489.2016.1173227DOI Listing
October 2016

Bu-shen-he-mai-fang (HMF) Decoction Inhibits Atherosclerosis by Improving Antioxidant and Anti-Inflammatory Activities in ApoE-deficient Mice.

Int J Biomed Sci 2014 Dec;10(4):258-64

Department of Cardiology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, China; ; The Key Laboratory of Cardiovascular Remodeling and Function Research, Shandong University Qilu Hospital, Jinan, Shandong, China; ; Department of Cardiology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.

Objective: To observe the effects of Bu-shen- he- mai- fang (HMF) on experimental atherosclerosis in ApoE-deficient mice.

Materials An Methods: Thirty male ApoE-deficient mice were randomly divided into 3 groups (10 mice per group) as follows: one group received the standard high- cholesterol diet (high- cholesterol group, HC); Another group received high- cholesterol diet supplemented with HMF decoction 1.37 g/kg/day; the third group received a high- cholesterol diet, supplemented with atrovastatin 5 mg/kg/day for 8 weeks. The extent of atherosclerosis, the expression of LOX-1 protein and macrophage infiltration were evaluated by H&E, oil red O staining, and immunohistochemical staining. SOD was also measured by a spectrophotometer.

Results: The degree of atherosclerosis was significantly lower in HMF group and atrovastatin group than that in high-cholesterol group. The expression of LOX-1 protein and macrophage filtration were significantly lower in HMF group and atrovastatin group than that in high-cholesterol group. Also, the SOD was higher in HMF group and atrovastatin group than that in high-cholesterol group.

Conclusion: The results suggested that HMF significantly inhibited early atherosclerotic lesions by inhibiting inflammatory response and decreasing the generation of ROS.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289700PMC
December 2014

Curcumin Attenuates Angiotensin II-Induced Abdominal Aortic Aneurysm by Inhibition of Inflammatory Response and ERK Signaling Pathways.

Evid Based Complement Alternat Med 2014 6;2014:270930. Epub 2014 Nov 6.

Department of Cardiology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, China.

Background and Objectives. Curcumin has long been used to treat age-related diseases, such as atherosclerosis and coronary heart disease. In this study, we explored the effects of curcumin on the development of abdominal aortic aneurysm (AAA). Methods. ApoE(-/-) mice were randomly divided into 3 groups: AngII group, AngII + curcumin (AngII + Cur) group (100 mg/kg/d), and the control group. Miniosmotic pumps were implanted subcutaneously in ApoE(-/-) mice to deliver AngII for 28 days. After 4-week treatment, abdominal aortas with AAA were obtained for H&E staining, immunohistochemistry, and Western blotting. Results. The results showed that curcumin treatment significantly decreased the occurrence of AAA. The levels of macrophage infiltration, monocyte chemoattractant protein-1 (MCP-1), and tumor necrosis factors-α (TNF-α) were significantly lower in AngII + Cur group than those in AngII group (all P < 0.01). The level of superoxide dismutase (SOD) was significantly higher in AngII + Cur group than those in AngII group (P < 0.01). The ERK1/2 phosphorylation in AngII + Cur group was significantly lower than that in AngII group (P < 0.01). Conclusions. These results suggested that curcumin can inhibit the AngII-induced AAA in ApoE(-/-) mice, whose mechanisms include the curcumin anti-inflammation, antioxidative stress, and downregulation of ERK signaling pathway.
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http://dx.doi.org/10.1155/2014/270930DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4241315PMC
November 2014

The effect of transportation on the expression of heat shock proteins and meat quality of M. longissimus dorsi in pigs.

Meat Sci 2009 Nov 21;83(3):474-8. Epub 2009 Jun 21.

College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China.

This study investigates the effect of different transport times on meat quality and the correlation between meat quality and Hsp expression in M. longissimus dorsi (LD) of pigs. After transportation for 1h, 2h or 4h, respectively, blood plasma creatine kinase (CK) and lactate dehydrogenase (LDH) increased. The LD meat from 1h and 2h transported pigs had lower initial and ultimate pH values (pHi and pHu, respectively), higher drip loss and L(∗) values compared to controls, indicating a higher likelihood of pale, soft and exudative (PSE) meat. Meat quality was lower after 2h compared to 1h or 4h of transport. All four Hsps tested (alpha-B-crystalline, Hsp27, Hsp70 and Hsp90) by ELISA in the LD tissue of pigs tended to decrease after transportation. One possible mechanism resulting in poor meat quality in the LD after transport seems to be a decline in Hsp expression.
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http://dx.doi.org/10.1016/j.meatsci.2009.06.028DOI Listing
November 2009
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