Publications by authors named "Qiang Shi"

409 Publications

Human Bone Marrow Mesenchymal Stem Cell-Derived Exosomes Attenuate Blood-Spinal Cord Barrier Disruption via the TIMP2/MMP Pathway After Acute Spinal Cord Injury.

Mol Neurobiol 2021 Sep 23. Epub 2021 Sep 23.

Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.

After spinal cord injury (SCI), destruction of the blood-spinal cord barrier (BSCB) results in infiltration of blood cells, such as neutrophils and macrophages, leading to permanent neurological dysfunction. Previous studies have shown that human bone marrow mesenchymal stem cell (BMSC)-derived exosomes have a beneficial neuroprotective effect in SCI models. However, whether BMSC-Exos contribute to the integrity of the BSCB has not been clarified. The purpose of this study was to investigate the mechanism of BMSC-Exo-induced changes in the permeability of the BSCB after SCI. Here, we first used BMSC-Exos to treat an SCI rat model, showing that BMSC-Exos can inhibit BSCB permeability damage and improve spontaneous repair. Next, we found that tissue inhibitors of matrix metalloproteinase 2 (TIMP2) have been shown to play an important role in the function of BMSC-Exos by inhibiting the matrix metalloproteinase (MMP) pathway, thereby reducing the reduction of cell junction proteins. Therefore, we constructed siTIMP2 to knock out TIMP2 in BMSC-Exos, which caused the activity of BMSC-Exos to be significantly weakened. Finally, we constructed an in vitro model of BSCB with HBMECs and verified that TIMP2 in BMSC-Exos in vitro can also alleviate BSCB damage. This proof-of-principle study demonstrates that BMSC-Exos can preserve the integrity of the BSCB and improve functional recovery after SCI through the TIMP2/MMP signaling pathway.
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http://dx.doi.org/10.1007/s12035-021-02565-wDOI Listing
September 2021

Catalysis in Single Crystalline Materials: From Discrete Molecules to Metal-Organic Frameworks.

Chem Asian J 2021 Sep 21. Epub 2021 Sep 21.

Advanced Materials Institute, Qilu University of Technology, Shandong Academy of Sciences), Jinan, 250014, P. R. China.

Catalysis is one of the key techniques for people's modern life. It has created numerous essential chemicals such as biomedicines, agricultural chemicals and unique materials. Heterogeneous catalysis is the new emerging method with reusable catalysts. Among heterogenous catalysis patterns developed so far, single crystalline catalysis has become the promising one owing to its high catalytic density and selectivity resulted by the inherent porosity, orderliness of the lattices and permeability. These crystalline catalysts could be used in various reactions such as photo-dimerization, Diels-Alder reaction, CO transformation and so on. In this review, we highlighted the reported works about the single crystalline catalysts. Both discrete small molecules and metal-organic frameworks (MOFs) have been used to prepare single crystals for catalysis. For discrete molecules based crystalline catalysts, coordinated and covalent molecules have been used. There were more catalytic modes in crystalline MOF catalysts. Three patterns were identified in this review: single crystalline MOFs i) without catalytic sites, ii) with inherent catalytic features and iii) with introducing catalytic units by post synthetic modification. Based on these examples, this review committed to provide the inspirations for the further design and application of single crystalline materials.
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http://dx.doi.org/10.1002/asia.202100957DOI Listing
September 2021

[Research on the classification criteria of femoral intertrochanteric fractures based on irreducibility or not].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 Sep;35(9):1086-1092

Department of Traumatic Orthopedics, Affiliated Hospital of Jining Medical University, Jining Shandong, 272029, P.R.China.

Objective: To formulate the classification criteria of femoral intertrochanteric fractures based on irreducibility or not in order to predict the difficulty of fracture recovery.

Methods: A clinical data of 244 patients with closed femoral intertrochanteric fractures admitted between January 2017 and March 2020 was retrospectively analyzed. There were 116 males and 128 females with an average age of 77.9 years (range, 45-100 years). The cause of injury included falling in 190 cases, traffic accident in 36 cases, smashing in 13 cases, and falling from height in 5 cases. The time from injury to operation was 1-14 days (mean, 3.6 days). According toAO/Orthopaedic Trauma Association (AO/OTA) classification, the fractures were classified as type 31-A1 in 38 cases, type 31-A2 in 160 cases, and type 31-A3 in 46 cases. According to whether the recovery difficulty occurred after intraoperative closed traction reset, the patients were divided into reducible-group and irreducible-group; combined with the literature and preoperative imaging data of two groups, the classification criteria of femoral intertrochanteric fractures was formulated based on the irreducibility or not. The 244 fractures were classified by the doctors who did not attend the operation according to the classification criteria, predicted the difficulty of fracture reduction, and compared with the actual intraoperative reduction situation.

Results: The 244 patients were divided into reducible-group ( =164, 67.21%) and irreducible-group ( =80, 32.79%) according to the intraoperative difficulty of reduction. Comparing the imaging data and characteristics of the two groups, and formulating the classification criteria of femoral intertrochanteric fractures based on irreducibility or not, the fractures were mainly divided into two categories of irreducibility and reducibility. The fractures of irreducibility category was divided into typesⅠ-Ⅴ, among which type Ⅲ was divided into subtypes 1-4; the fractures of reducibility category was divided into typesⅠand Ⅱ. Compared with the actual intraoperative evaluation results, the total accuracy rate of the doctors who did not attend the operation was 81.15% (198/244) based on the classification criteria of femoral intertrochanteric fractures. The accuracy rate of irreducibility category was 65.74% (71/108), and the reducibility category was 93.38% (127/136). All patients were followed up 13-25 months, with an average of 17.6 months. All fractures healed except 2 cases died of infection.

Conclusion: The classification criteria of femoral intertrochanteric fractures based on irreducibility or not can accurately predict the reducible cases preoperatively, and most of the irreducible cases can be correctly predicted in a wider way. But the classification criteria still need to be further improved and supplemented.
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http://dx.doi.org/10.7507/1002-1892.202103233DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8444130PMC
September 2021

Desalination of duck egg white by biocoagulation to obtain peptide-ferrous chelate as iron delivery system: Preparation, characterization, and Fe2+ release evaluation in vitro.

J Food Sci 2021 Oct 14;86(10):4678-4690. Epub 2021 Sep 14.

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, People's Republic of China.

The annual output of salted duck egg white (SDEW) is estimated to be over 1.5 million tons in China, most of which is discarded due to high salt content. This has led to serious waste and environmental impact. Therefore, we developed an eco-friendly biocoagulation separation technology by combining chitosan and sodium alginate in order to produce a novel iron-binding peptide (DPs-Fe2+) from SDEW. The structure of DPs-Fe2+ was characterized by ultraviolet-visible spectroscopy, fluorescence spectroscopy, and Fourier transform infrared spectroscopy, followed by measuring DPs-Fe2+ response in a simulated digestion/Caco-2 cell model. Results showed that chitosan and sodium alginate complex could remove 91.21% of salt from SDEW, and the protein recovery rate reached 95.50%. Characterization results indicated that DPs bonded with Fe2+ to form a soluble chelate. Moreover, Caco-2 cell monolayer model indicated that the transport rate of Fe2+ was as high as 10.02% at 0.1 mg/ml concentration of digested chelates. The results demonstrate the potential application of DPs as a novel carrier for enhancing iron absorption. This research contributes to the development of an effective industrial desalination method and highlights an opportunity for recycling an otherwise discarded processing byproduct. PRACTICAL APPLICATION: Salted duck egg whites (SDEW) are the primary byproduct of salted egg yolk production, most of which is discarded due to high salt content. Hence, efficient utilization of the high-value proteins in SDEW is an urgent problem that must be resolved. Herein, we developed an effective industrial desalination method by combining chitosan and sodium alginate, which achieved excellent SDEW desalination and protein recovery. Furthermore, we produced a novel iron-binding peptide (DPs-Fe2+), which enhanced the transportation and absorption of Fe2+ in Caco-2 cell model, suggesting its potential as an iron supplement.
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http://dx.doi.org/10.1111/1750-3841.15902DOI Listing
October 2021

Effects of serum from mismatched patients with solid organ transplantation on the activation of microvascular cultures isolated from adipose tissues.

Transpl Immunol 2021 Sep 8;69:101462. Epub 2021 Sep 8.

Transplant Immunology, Baylor University Medical Center, 3500 Gaston Ave, 4th Floor of the Y Wing, RM# L-0470, Dallas, TX 75246, United States of America. Electronic address:

Background: Aggregating the human leukocyte antigen (HLA) Class I antigens on the endothelial membrane has been known to elicit an activation, an underlying mechanism of chronic rejection in organ transplant recipients. The current study aims at examining the endothelial responses using HLA typed microvascular cultures from human adipose tissues upon exposure to the serum that contain corresponding antibodies collected from mismatched transplant recipients.

Methods: We have successfully cultured 30 microvascular cultures and typed their HLAs. They are functionally competent to respond to inflammatory TNF-α stimulation and the aggregating monoclonal antibody against HLA Class I. The post-transplantation serum was collected either from the recipients with pathologically diagnosed chronic rejection or from the recipients without rejection. We determined their activation either by double-staining the endothelial cells in crude cultures with flow cytometry or by quantifying cytokine releases in purified endothelial cells using ELISA.

Results: Under our current protocol, adipose tissue cultures are functionally intact in regard to its responses to TNF-alpha and anti-HLA Class I antibody. We observed that the post-transplantation serum with rejection contained the pathogenic antibodies and led to proinflammatory activation, as demonstrated by not only increased CD54+/CD31+ and CD106+/CD31+ cell counts but also inflammatory cytokine releases including MCP-1, IL-8 and RANTES.

Conclusion: This methodological study provides the feasibility of examining the pathogenicity of the alloantibodies in mis-transplant serum. Potentially, the endothelial activation elicited as a result of exposure can be used as an alternative readout for chronic rejection.

Significance: We prototype an ex vivo model that enables us to examine whether allogenic antibodies from the recipient can functionally activate microvascular endothelial cells from the donor adipose tissues. This system can be further developed as crossmatch using cellular responses as readouts for chronic rejection for post-transplant surveillance.
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http://dx.doi.org/10.1016/j.trim.2021.101462DOI Listing
September 2021

A Wide Range of High Signal Intensities on Brain Image in Adult Mycoplasma Pneumoniae-Associated Mild Encephalitis/Encephalopathy with a Reversible Splenial Lesion.

Neurol India 2021 Jul-Aug;69(4):1112-1113

Department of Neurology, The Chinese People's Liberation Army General Hospital; Department of National Clinical Research Center of Geriatrics Disease, the Chinese People's Liberation Army General Hospital, Beijing, China.

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http://dx.doi.org/10.4103/0028-3886.325373DOI Listing
September 2021

Mn ions substitution inducing improvement of optical performances in ZnAlO: Cr phosphors: Energy transfer and ratiometric optical thermometry.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Aug 27;264:120321. Epub 2021 Aug 27.

School of Physical Science and Information Technology, Liaocheng University, Liaocheng 252059, China; Shandong Key Lab. of Optical Communication Science and Technology, Liaocheng University, Liaocheng 252059, China.

ZnMnAlO:0.1 mol% Cr (0.04≤x≤0.16) phosphors with single spinel phase were synthesized by using sol-gel method and the structure, optical and temperature sensing performances were reported herein. The results of X-ray photoelectron spectra indicate that the inversion defects related to octahedral Zn are reduced and the crystal field surrounding Al changes with Mn doping in ZnAlO lattices. Mn/Cr co-doped ZnAlO nanophosphors reveal a green emission band assigned to Mn and a series of red emission peaks assigned to Cr, respectively. With the concentration of Mn increasing, the intensity of zero phonon line (R line) assigned to Cr increases, reaching the maximum at the optimal Mn concentration of x=0.14. The energy transfer from Mn to Cr is confirmed with the energy transfer efficiency of 83%. The separation between E(e) and E(t) of Cr is enlarged due to Mn dopants giving rise to a change of crystal field. The luminous intensity ratio between two separated emission peaks at 685 nm (R) and 689 nm (R) reveals an obvious temperature dependence. The relative sensitivity changes from 3.7 %K to 0.25 %K with the temperature increasing from 80 K to 310 K, which is much larger than that of ZnAlO:Cr nanophosphors without Mn, indicating its good application prospect in optical thermometry.
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http://dx.doi.org/10.1016/j.saa.2021.120321DOI Listing
August 2021

Correction to: Improved submucosal tunneling endoscopic resection with slant tunnel for submucosal tumors in proximal esophagus.

Surg Endosc 2021 Aug 31. Epub 2021 Aug 31.

Endoscopy Center, Zhongshan Hospital of Fudan University, Shanghai, 200032, China.

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http://dx.doi.org/10.1007/s00464-021-08624-6DOI Listing
August 2021

Anticancer Effect of Rh2, a Histone Deacetylase Inhibitor, in HepG2 Cells and HepG2 Cell-Derived Xenograft Tumors Occurs via the Inhibition of HDACs and Activation of the MAPK Signaling Pathway.

Asian Pac J Cancer Prev 2021 Aug 1;22(8):2529-2539. Epub 2021 Aug 1.

Laboratory of Stem Cell and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing, China.

Purpose: To investigate the effect of 20(S)-ginsenoside Rh2 (Rh2) on anti HepG2 liver cancer cells and HepG2 cell-derived xenograft tumors, and explore the underlying mechanisms.

Materials And Methods: The activity of total HDACs and HAT were assessed with a HDACs colorimetric kit. Expression of HDAC1, HDAC2, HDAC6, p-ERK, ERK, p-P38, P38, p-JNK and JNK proteins was tested by Western blotting.H3K9 and H3K14 proteins were also checked by immunofluorescence, changes in cell cycle distribution with flow cytometry, cell apoptosis with annexin V-FTIC/PI double staining. Activity of Renilla luciferase (HIF) was detected using the Luciferase Reporter Assay system reagent. Gene expression for CyclinD1, Bcl-2, Bax, HIF, IL-1, IL-6, IL-10 and TNF-α was tested by q-PCR. Expression levels of CD31 and Ki-67 was tested by immunohistochemical staining.

Results: Total HDAC activity was decreased and total histone acetyltransferase (HAT)activity was increased in a time-dependent manner. Expression of HDAC1 and p-JNK proteins was significantly increased, expression levels of p-ERK was decreased. H3K9 and H3K14 fluorescence protein were increased. Flow cytometric analysis of the cell cycle revealed that the percentage of cells in the G0/G1 phase in the treatment group(64.35±1.36%) was significantly increased compared with the untreated group(61.61±1.23%).The apoptotic rate of the HepG2 group was 10.03±1.92%, which increased to 17.87±1.67% in the treatment group. Expression levels of the transcription factor HIF were also increased in HepG2 cells following induction by Rh2. Expression of CyclinD1 and Bcl-2 at the genetic level was significantly decreased, while expression levels of Bax, HIF, IL-1, IL-6, IL-10 and TNF-α was increased. In vivo, the expression levels of both CD31 and Ki-67 proteins were significantly down-regulated in the treatment group compared with the control group.

Conclusions: The effects of Rh2 were suggested to occur through the inhibition of total HDAC activity, which subsequently induced MAPK signaling and down-regulated the expression of HIF.
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http://dx.doi.org/10.31557/APJCP.2021.22.8.2529DOI Listing
August 2021

Identification of HSC/MPP expansion units in fetal liver by single-cell spatiotemporal transcriptomics.

Cell Res 2021 Aug 2. Epub 2021 Aug 2.

State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Limited knowledge of cellular and molecular mechanisms underlying hematopoietic stem cell and multipotent progenitor (HSC/MPP) expansion within their native niche has impeded the application of stem cell-based therapies for hematological malignancies. Here, we constructed a spatiotemporal transcriptome map of mouse fetal liver (FL) as a platform for hypothesis generation and subsequent experimental validation of novel regulatory mechanisms. Single-cell transcriptomics revealed three transcriptionally heterogeneous HSC/MPP subsets, among which a CD93-enriched subset exhibited enhanced stem cell properties. Moreover, by employing integrative analysis of single-cell and spatial transcriptomics, we identified novel HSC/MPP 'pocket-like' units (HSC PLUS), composed of niche cells (hepatoblasts, stromal cells, endothelial cells, and macrophages) and enriched with growth factors. Unexpectedly, macrophages showed an 11-fold enrichment in the HSC PLUS. Functionally, macrophage-HSC/MPP co-culture assay and candidate molecule testing, respectively, validated the supportive role of macrophages and growth factors (MDK, PTN, and IGFBP5) in HSC/MPP expansion. Finally, cross-species analysis and functional validation showed conserved cell-cell interactions and expansion mechanisms but divergent transcriptome signatures between mouse and human FL HSCs/MPPs. Taken together, these results provide an essential resource for understanding HSC/MPP development in FL, and novel insight into functional HSC/MPP expansion ex vivo.
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http://dx.doi.org/10.1038/s41422-021-00540-7DOI Listing
August 2021

The Conditioned Medium of Lactobacillus rhamnoides GG Regulates Microglia/Macrophage Polarization and Improves Functional Recovery after Spinal Cord Injury in Rats.

Biomed Res Int 2021 10;2021:3376496. Epub 2021 Jul 10.

Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated No.6 People's Hospital, 600 Yishan Road, Shanghai 200233, China.

Lactobacillus rhamnoides, a human intestinal colonizer, can act through various pathways to induce microglia/macrophages to produce cytokines and to polarize microglia/macrophages to different phenotypes to reduce the inflammatory response. In this article, we evaluated the treatment potential of the Lactobacillus rhamnoides GG conditioned medium (LGG-CM) in rat model with SCI (acute spinal cord injury), including functional, neurophysiological, and histological outcomes and the underlying neuroprotective mechanisms. In our experiment, LGG-CM (30 mg/kg) was injected directly into the injury site in rats immediately after SCI. Measured by the BBB scale (Basso, Beattie, and Bresnahan locomotor rating scale) and inclined plane test, rats in the LGG-CM-treated group showed better locomotor scores. Moreover, compared to the vehicle treatment group, LGG-CM increased the mRNA level of the M2 marker (CD206), and decreased that of the M1 marker (iNOS). Western blot assays showed that LGG-CM-treated SCI rats had a higher grayscale ratio of p65 and a lower ratio of p-IB/IB. Our study shows that local injection of LGG-CM after acute SCI can inhibit inflammatory responses and improve motor function recovery. These effects may be related with the inhibition to the NF-B (The nuclear factor-kappa B) signal pathway which leads to M2 microglia/macrophage polarization.
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http://dx.doi.org/10.1155/2021/3376496DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8289592PMC
September 2021

Case Report: Diagnosis of Human Alveolar Echinococcosis via Next-Generation Sequencing Analysis.

Front Genet 2021 9;12:666225. Epub 2021 Jul 9.

Department of Neurology, The First Medical Center, Chinese PLA General Hospital, Beijing, China.

Alveolar echinococcosis (AE) is a rare parasitic disease caused by the infection of . AE may mimic malignancy both in clinical presentation and radiological imaging, which is often misdiagnosed as metastatic tumor. Recently, next-generation sequencing (NGS) technologies are increasingly being used to address a diverse range of biological questions. Here, we describe a rare case of alveolar echinococcosis diagnosed by pan-pathogen screening, using next-generation sequencing. To the best of our knowledge, this is the first reported case of AE which was definitely diagnosed relying NGS of cerebrospinal fluid (CSF). A 33-year-old man presented with repeat seizure and progressive headache for six months. Head magnetic resonance imaging (MRI) showed multiple masses with edema. Lung and abdominal computer tomography (CT) revealed multiple masses in bilateral lung, liver and the right adrenal gland. Bacterial, tuberculosis and fungal infection were excluded by CSF examination. Repeated target biopsy on the masses in the lung and liver showed as fibrous connective tissue without positive findings. NGS of CSF was performed and detected nucleic acid sequences of . Consequently, the patient has accepted 1-year albendazole therapy. His case was followed up through imaging procedures. The next-generation sequencing of CSF is a reliable and sensitive diagnostic method for the detection of pathogenic microorganisms, and may allow the accurate diagnosis of alveolar echinococcosis. In view of this case, we recommend NGS as a potential tool for diagnosis of cerebral AE, especially if repeated biopsies are negative.
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http://dx.doi.org/10.3389/fgene.2021.666225DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299116PMC
July 2021

Comparison of IOL-Master 700 and IOL-Master 500 biometers in ocular biological parameters of adolescents.

Int J Ophthalmol 2021 18;14(7):1013-1017. Epub 2021 Jul 18.

Department of Ophthalmology, the First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.

Aim: To compare the differences and consistency of IOL-Master 700 biometers applying swept optical coherence tomography with the conventional IOL-Master 500 applying partial coherence interference in terms of the ocular biological parameters in adolescents with ametropia.

Methods: A total of 110 adolescents (110 eyes) with ametropia were collected, including 55 males and 55 females; age 10.69±2.81y. Ocular biological measurements were taken by IOL-Master 700 and IOL-Master 500 respectively to obtain biological parameters including axial length (AL), mean corneal anterior surface keratometry (K), anterior chamber depth (ACD), and horizontal corneal diameter (WTW). Paired -test was used to compare the differences between the two instruments. The intra-group correlation coefficient (ICC) and the Bland-Altman analysis were used to evaluate the consistency of parameter measurements between the two instruments for the four biological parameters.

Results: Statistical analysis showed that there was no significant difference in the K value measured by IOL-Master 700 and IOL-Master 500 (=-1.644, =0.116). The average differences of the AL, ACD, and WTW distances between the two instruments are 0.028, 0.101 and 0.064 mm respectively, and the differences are statistically significant (=2.644, 12.505, 3.911, <0.001). The consistency study results indicated high correlation in the measurement of AL, K, ACD and WTW between the two instruments (ICC=0.994, 0.873, 0.927, 0.912).

Conclusion: The novel biometric instrument IOL-Master 700 makes no difference with IOL-Master 500 in the measurement of K. There are some differences in the values of AL, ACD, and WTW. However, the two instruments show good consistency in these four biological measurements. The measured values of K are interchangeable between the instruments. These two types of biometrics can be used as mutual reference in consideration of that the differences in AL, ACD, and WTW measurements are not sufficient to produce clinically meaningful differences.
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http://dx.doi.org/10.18240/ijo.2021.07.08DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243180PMC
July 2021

Efficient propagation of the hierarchical equations of motion using the Tucker and hierarchical Tucker tensors.

J Chem Phys 2021 May;154(19):194104

Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Zhongguancun, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China; and Physical Science Laboratory, Huairou National Comprehensive Science Center, Beijing 101407, China.

We develop new methods to efficiently propagate the hierarchical equations of motion (HEOM) by using the Tucker and hierarchical Tucker (HT) tensors to represent the reduced density operator and auxiliary density operators. We first show that by employing the split operator method, the specific structure of the HEOM allows a simple propagation scheme using the Tucker tensor. When the number of effective modes in the HEOM increases and the Tucker representation becomes intractable, the split operator method is extended to the binary tree structure of the HT representation. It is found that to update the binary tree nodes related to a specific effective mode, we only need to propagate a short matrix product state constructed from these nodes. Numerical results show that by further employing the mode combination technique commonly used in the multi-configuration time-dependent Hartree approaches, the binary tree representation can be applied to study excitation energy transfer dynamics in a fairly large system including over 10 effective modes. The new methods may thus provide a promising tool in simulating quantum dynamics in condensed phases.
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http://dx.doi.org/10.1063/5.0050720DOI Listing
May 2021

Muscular pathological features in patients with myasthenia gravis.

Clin Neuropathol 2021 Jul 8. Epub 2021 Jul 8.

Objective: To analyze muscle histopathology of myasthenia gravis (MG) patients and further explore the underlying mechanism comparing with previous literature.

Materials And Methods: We analyzed the clinicopathological features of 8 MG patients who had muscle biopsy examinations.

Results: Eight patients with a diagnosis of MG were retrospectively recruited from the Chinese PLA General Hospital. One patient had positive anti-MuSK antibodies, 5 patients had positive anti-AChR antibodies (1 of whom had additional positive anti-Titin antibodies), and 2 patients were seronegative. Seronegative-MG presented normal muscle histology, occasionally with lipid deposition. Small angular atrophy (mainly in type II fibers) and necrosis in H & E stain were found in AChR-MG, furthermore, patterns of polymyositis (PM) could be found in AChR-MG with anti-Titin antibodies. Mitochondrial abnormalities were found only in MuSK-MG.

Conclusion: Muscle histological abnormalities mimicking myopathy may be found in MG patients. Patients with different antibodies present with different muscle histopathology. PM pattern pathology is a special pattern of muscle histology in MG that should not be misdiagnosed. Our study has extended the muscle pathological features of MG in addition to deepening the understanding of MG.
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http://dx.doi.org/10.5414/NP301382DOI Listing
July 2021

Exploration of yellow-emitting phosphors for white LEDs from natural resources.

Appl Opt 2021 Jun;60(16):4716-4722

White light-emitting diodes (LEDs) are widely used in various lighting fields as a part of energy-efficient technology. However, some shortcomings of luminescent materials for white LEDs, such as complexity of synthesis, high cost, and harmful impact on the environment, limit their practical applications to a large extent. In this respect, the present work aims to study the ability of using Berberine (BBR) chloride extracted from Rhizoma coptidis and Phellodendron Chinese herbs as yellow phosphor for white LEDs. For this, white LEDs were successfully fabricated by applying 0.006 g of BBR chloride onto the blue LED chips (450 nm). The produced LEDs exhibited good luminescence properties at a voltage of 2.4 V along with eco-friendly characteristics and low cost. The Commission International de l'Eclairage chromaticity, the correlated color temperature, and the color rendering index were determined to be (${x} = {0.32}$, ${y} = {0.33}$), 5934 K, and 74, respectively. Therefore, BBR chloride is a suitable environmentally friendly and easily accessible yellow phosphor for white LEDs.
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http://dx.doi.org/10.1364/AO.424108DOI Listing
June 2021

Efficacy Evaluation of 3D Navigational Template for Salter Osteotomy of DDH in Children.

Authors:
Jie Yu Qiang Shi

Biomed Res Int 2021 22;2021:8832617. Epub 2021 May 22.

Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.

Background: The aim of this study is to retrospectively evaluate the efficacy of 3D navigational template for Salter osteotomy of DDH in children.

Methods: Thirty-two consecutive patients with DDH who underwent Salter osteotomy were evaluated between July 2014 and August 2017, and they were divided into the conventional group ( = 16) and navigation template group ( = 16) according to different surgical methods. The corrective acetabular degrees, radiation exposure, and operation time were compared between the two groups.

Results: No nerve palsy or redislocation was reported in the navigation template group. Compared with the conventional group, the navigation template group had the advantages of more accurate acetabular degrees, less radiation exposure, and shorter operation time ( < 0.05). Meanwhile, the navigation template group achieved a better surgical outcome than the conventional group (McKay, = 0.0293; Severin, = 0.0949).

Conclusions: The 3D navigational template for Salter osteotomy of DDH is simple and effective, which could be an alternative approach to improve the Salter osteotomy accuracy and optimize the efficacy.
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http://dx.doi.org/10.1155/2021/8832617DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166498PMC
September 2021

Transient oculomotor paralysis after cerebral angiography: A case report.

Medicine (Baltimore) 2021 Jun;100(22):e26242

Department of Neurology.

Rationale: A special case of transient oculomotor nerve palsy after cerebral angiography.

Patient Concerns: A 55-year-old man developed oculomotor nerve dysfunction after right radial artery puncture angiography.

Diagnoses: Cerebral angiography-induced oculomotor nerve palsy.

Interventions: According to the patient's disease state, intravenous drip of dexamethasone 10 mg/d.

Outcomes: Magnetic resonance imaging (MRI) showed no abnormalities, and the patient recovered completely after 48 hours of hormone therapy.

Lessons: Transient eye palsy caused by contrast agent encephalopathy is a clinically rare neurological dysfunction caused by adverse effects of contrast agents. Early prevention and correct treatment are critical.
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http://dx.doi.org/10.1097/MD.0000000000026242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183771PMC
June 2021

Improved submucosal tunneling endoscopic resection with slant tunnel for submucosal tumors in proximal esophagus.

Surg Endosc 2021 07 2;35(7):4055-4060. Epub 2021 Jun 2.

Endoscopy Center, Zhongshan Hospital of Fudan University, Shanghai, 200032, China.

Background: The improved submucosal tunneling endoscopic resection (STER) with slant tunnel was created by our group innovatively for submucosal tumors (SMTs) in the proximal esophagus. This study aimed to provide the preliminary results of the improved STER from our center.

Methods: The key step of the improved STER is establishing a slant tunnel instead of a vertical tunnel. After a longitudinal incision was made proximally in the inclined top to the tumor, a submucosal tunnel was established from the incision to the SMT slantingly. 28 patients undergoing STER with slant tunnel were enrolled in the retrospective study. Clinical results including en bloc resection, curative resection and complication were collected.

Results: All the submucous tumors located at proximal esophagus originated from muscularis propria were successfully resected by the innovative STER. Tumor size ranged from 18-43 mm, with 96.4% (27/28) en bloc resection rate and 92.9% (26/28) curative rate. Three patients suffered complication, 1 patient with mild pleural effusion and another 2 patients with fever for one day. All of the complications were cured by conservative treatment.

Conclusions: STER with slant tunnel seems to provide an optional treatment for tumors in proximal esophagus.
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http://dx.doi.org/10.1007/s00464-021-08503-0DOI Listing
July 2021

Comparative evaluation of pinning and cast fixation vs. external fixation after lateral closing-wedge osteotomy for cubitus varus in children.

J Shoulder Elbow Surg 2021 May 27. Epub 2021 May 27.

Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, China. Electronic address:

Background: Cubitus varus has been regarded as poor functional and cosmetic consequences of supracondylar humerus fracture in children. The aim of this study was to assess the clinical and radiologic outcomes of cubitus varus based on fixation methods: K-wire and cast fixation or external fixation.

Methods: 40 consecutive patients with cubitus varus secondary to supracondylar fractures were enrolled between October 2015 and December 2018. Following lateral closing-wedge osteotomy, the method of K-wire and cast fixation was chosen as group A (n = 21) while the method of external fixation was defined as group B (n = 19), retrospectively. We measured the bony union, elbow joint range of motion, and carrying angle. The clinical and radiographic results were assessed according to the Bellemore criteria.

Results: No significant difference between the 2 groups in terms of age, gender, operation duration, union time, and postoperative elbow range of motion (P > .05). A significant difference was noted in postoperative carrying angle and results according to Bellemore criteria in group B (P < .05). No nonunion, myositis ossificans, or neurovascular injury was found at follow-up in 2 groups. In group A, revision surgery was needed for residual varus in 1 patient and lateral condylar prominence was found in 2 patients. In group B, a superficial pin-site infection occurred in 2 patients who were treated successfully with oral antibiotics.

Conclusions: Both K-wire and external fixation after lateral closing-wedge osteotomy are reliable and effective for the treatment of cubitus varus in children. Compared with K-wire method, external fixation could achieve better functional and cosmetic results with a shorter learning curve.
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http://dx.doi.org/10.1016/j.jse.2021.04.042DOI Listing
May 2021

Study on polysaccharide polyelectrolyte complex and fabrication of alginate/chitosan derivative composite fibers.

Int J Biol Macromol 2021 Aug 26;184:181-187. Epub 2021 May 26.

State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.

Sodium alginate (SA) blending with quaternary ammonium chitosan (QAC) polysaccharide polyelectrolyte complex (PEC) system was chosen to research the binary blending of anionic and cationic polyelectrolytes in detail and to fabricate SA/QAC composite fibers. The potential charge and the rheology of the PEC solution were characterized through Zeta Laser Particle Size Analyzer and DV-C Rotary Rheometer, the structure and properties of the composite fiber were examined by FT-IR, XRD, SEM, EDS, and YG004 single fiber strength meter. The results showed that as the mass ratio of SA to QAC increased from 0/1 to 10/1, the state of the binary solution in water changed from transparent uniform solution to turbid solution with flocculent precipitate, then back to uniform solution, accompanied by the electrical potential change. Moreover, the electrical potential also depended on salt in solution. By using this uniform PEC solution with the mass ratio of SA to QAC 10/1 and concentration 5.5 wt% in water, SA/QAC composite fibers with excellent performances of breaking strength 2.37 cN·dtex and breaking elongation 14.11%, good antibacterial and hydrophobic properties were fabricated via green wet-spinning process. The FT-IR and EDS determination indicated there formed egg-box between SA and Ca, cross-linked network between glutaraldehyde(GA) and SA, QAC, respectively. Depending on its mechanical, natural, and antibacterial properties, the SA/QAC composite fiber has advantages in wound dressing, medical gauze, medical absorbable suture, and tissue engineering.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.05.150DOI Listing
August 2021

Understanding the Large Kinetic Isotope Effect of Hydrogen Tunneling in Condensed Phases by Using Double-Well Model Systems.

J Phys Chem B 2021 Jun 25;125(22):5959-5970. Epub 2021 May 25.

Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Zhongguancun, Beijing 100190, China.

In recent years, many experiments have shown large kinetic isotope effects (KIEs) for hydrogen transfer reactions in condensed phases as evidence of strong quantum tunneling effects. Since accurate calculation of the tunneling dynamics in such systems still present significant challenges, previous studies have employed different types of approximations to estimate the tunneling effects and KIEs. In this work, by employing model systems consisting of a double-well coupled to a harmonic bath, we calculate the tunneling effects and KIEs using the numerically exact hierarchical equations of motion (HEOM) method. It is found that hydrogen and deuterium transfer reactions in the same system may show rather different behaviors, where hydrogen transfer is dominated by tunneling between the two lowest vibrational states and deuterium transfer is controlled by excited vibrational states close to the barrier top. The simulation results are also used to test the validity of various approximate methods. It is shown that the Wolynes theory of dissipative tunneling gives a good estimation of rate constants in the over-the-barrier regime, while the nonadiabatic reaction rate theory based on the Landau-Zener formula is more suitable for deep tunneling reactions.
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http://dx.doi.org/10.1021/acs.jpcb.1c02851DOI Listing
June 2021

Cross-oncopanel study reveals high sensitivity and accuracy with overall analytical performance depending on genomic regions.

Genome Biol 2021 04 16;22(1):109. Epub 2021 Apr 16.

Greenwood Genetic Center, 106 Gregor Mendel Circle, Greenwood, SC, 29646, USA.

Background: Targeted sequencing using oncopanels requires comprehensive assessments of accuracy and detection sensitivity to ensure analytical validity. By employing reference materials characterized by the U.S. Food and Drug Administration-led SEquence Quality Control project phase2 (SEQC2) effort, we perform a cross-platform multi-lab evaluation of eight Pan-Cancer panels to assess best practices for oncopanel sequencing.

Results: All panels demonstrate high sensitivity across targeted high-confidence coding regions and variant types for the variants previously verified to have variant allele frequency (VAF) in the 5-20% range. Sensitivity is reduced by utilizing VAF thresholds due to inherent variability in VAF measurements. Enforcing a VAF threshold for reporting has a positive impact on reducing false positive calls. Importantly, the false positive rate is found to be significantly higher outside the high-confidence coding regions, resulting in lower reproducibility. Thus, region restriction and VAF thresholds lead to low relative technical variability in estimating promising biomarkers and tumor mutational burden.

Conclusion: This comprehensive study provides actionable guidelines for oncopanel sequencing and clear evidence that supports a simplified approach to assess the analytical performance of oncopanels. It will facilitate the rapid implementation, validation, and quality control of oncopanels in clinical use.
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http://dx.doi.org/10.1186/s13059-021-02315-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051090PMC
April 2021

A novel homozygous mutation in ATP13A2 gene causing pure hereditary spastic paraplegia.

Parkinsonism Relat Disord 2021 05 30;86:58-60. Epub 2021 Mar 30.

Department of Neurology, The First Medical Centre, Chinese PLA General Hospital, Beijing, China. Electronic address:

SPG78 is a subtype of hereditary spastic paraplegia(HSP) caused by ATP13A2 gene mutations. SPG78 was reported as complicated HSP in several cases, but was never associated with pure HSP. Here we report the first Chinese patient carrying a novel homozygous nonsense mutation in ATP13A2 presenting with pure HSP.
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http://dx.doi.org/10.1016/j.parkreldis.2021.03.020DOI Listing
May 2021

NLRP7 deubiquitination by USP10 promotes tumor progression and tumor-associated macrophage polarization in colorectal cancer.

J Exp Clin Cancer Res 2021 Apr 10;40(1):126. Epub 2021 Apr 10.

Endoscopy Center, Zhongshan Hospital of Fudan University, 180 Fenglin Road, Shanghai, 20032, People's Republic of China.

Background: NOD-like receptors affect multiple stages of cancer progression in many malignancies. NACHT, LRR, and PYD domain-containing protein 7 (NLRP7) is a member of the NOD-like receptor family, although its role in tumorigenesis remains unclear. By analyzing clinical samples, we found that NLRP7 protein levels were upregulated in colorectal cancer (CRC). We proposed the hypothesis that a high level of NLRP7 in CRC may promote tumor progression. Here, we further investigated the role of NLRP7 in CRC and the underlying mechanism.

Methods: NLRP7 expression in human CRC and adjacent non-tumorous tissues was examined by quantitative real-time polymerase chain reaction (qRT-PCR), western blotting, and immunohistochemistry. The effect of NLRP7 in CRC progression was investigated in vitro and in vivo. Proteins interacting with NLRP7 were identified by immunoprecipitation and mass spectrometry analysis while immunofluorescence staining revealed the cellular location of the proteins. Cellular ubiquitination and protein stability assays were applied to demonstrate the ubiquitination effect on NLRP7. Cloning and mutagenesis were used to identify a lysine acceptor site that mediates NLRP7 ubiquitination. Cytokines/chemokines affected by NLRP7 were identified by RNA sequencing, qRT-PCR, and enzyme-linked immunosorbent assay. Macrophage phenotypes were determined using qRT-PCR, flow cytometry, and immunohistochemistry.

Results: NLRP7 protein levels, but not mRNA levels, were upregulated in CRC, and increased NLRP7 protein expression was associated with poor survival. NLRP7 promoted tumor cell proliferation and metastasis in vivo and in vitro and interacted with ubiquitin-specific protease 10, which catalyzed its deubiquitination in CRC cells. NLRP7 stability and protein levels in CRC cells were modulated by ubiquitination and deubiquitination, and NLRP7 was involved in the ubiquitin-specific protease 10 promotion of tumor progression and metastasis in CRC. K379 was an important lysine acceptor site that mediates NLRP7 ubiquitination in CRC cells. In CRC, NLRP7 promoted the polarization of pro-tumor M2-like macrophages by inducing the secretion of C-C motif chemokine ligand 2. Furthermore, NLRP7 promoted NF-κB nuclear translocation and activation of C-C motif chemokine ligand 2 transcription.

Conclusions: We showed that NLRP7 promotes CRC progression and revealed an as-yet-unidentified mechanism by which NLRP7 induces the polarization of pro-tumor M2-like macrophages. These results suggest that NLRP7 could serve as a biomarker and novel therapeutic target for the treatment of CRC.
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http://dx.doi.org/10.1186/s13046-021-01920-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8035766PMC
April 2021

Cell-Free Book-Shaped Decellularized Tendon Matrix Graft Capable of Controlled Release of BMP-12 to Improve Tendon Healing in a Rat Model.

Am J Sports Med 2021 04 5;49(5):1333-1347. Epub 2021 Mar 5.

Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, China.

Background: Achilles tendon (AT) defects often occur in traumatic and chronic injuries. Currently, no graft can satisfactorily regenerate parallel tendinous tissue at the defect site to completely restore AT function.

Purpose: To develop a cell-free functional graft by tethering bone morphogenetic protein 12 (BMP-12) on a book-shaped decellularized tendon matrix (BDTM) and to determine whether this graft is more beneficial for AT defect healing than an autograft.

Study Design: Controlled laboratory study.

Methods: Canine patellar tendon was sectioned into a book shape and decellularized to fabricate a BDTM. The collagen-binding domain (CBD) was fused into the N-terminus of BMP-12 to synthesize a recombinant BMP-12 (CBD-BMP-12), which was tethered to the BDTM to prepare a cell-free functional graft (CBD-BMP-12/BDTM). After its tensile resistance, tenogenic inducibility, and BMP-12 release dynamics were evaluated, the efficacy of the graft for tendon regeneration was determined in a rat model. A total of 140 mature male Sprague-Dawley rats underwent AT tenotomy. The defect was reconstructed with reversed AT (autograft group), native BMP-12 tethered to an intact decellularized tendon matrix (IDTM; NAT-BMP-12/IDTM group), native BMP-12 tethered to a BDTM (NAT-BMP-12/BDTM group), CBD-BMP-12 tethered on an IDTM (CBD-BMP-12/IDTM group), and CBD-BMP-12 tethered on a BDTM (CBD-BMP-12/BDTM group). The rats were sacrificed 4 or 8 weeks after surgery to harvest AT specimens. Six specimens from each group at each time point were used for histological evaluation; the remaining 8 specimens were used for biomechanical testing.

Results: In vitro CBD-BMP-12/BDTM was noncytotoxic, showed high biomimetics with native tendons, was suitable for cell adhesion and growth, and had superior tenogenic inducibility. In vivo the defective AT in the CBD-BMP-12/BDTM group regenerated more naturally than in the other groups, as indicated by more spindle-shaped fibroblasts embedded in a matrix of parallel fibers. The biomechanical properties of the regenerated AT in the CBD-BMP-12/BDTM group also increased more significantly than in the other groups.

Conclusion: CBD-BMP-12/BDTM is more beneficial than autograft for healing AT defects in a rat model.

Clinical Relevance: The findings of this study demonstrate that CBD-BMP-12/BDTM can serve as a practical graft for reconstructing AT defects.
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http://dx.doi.org/10.1177/0363546521994555DOI Listing
April 2021

Characterization of the distributions of collagen and PGs content in the decellularized book-shaped enthesis scaffolds by SR-FTIR.

BMC Musculoskelet Disord 2021 Mar 1;22(1):235. Epub 2021 Mar 1.

Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.

Background: Bone-tendon interface (enthesis) plays a pivotal role in relaxing load transfer between otherwise structurally and functionally distinct tissue types. Currently, decellularized extracellular matrix (DEM) from enthesis provide a natural three-dimensional scaffold with tissue-specific orientations of extracellular matrix molecules for enthesis regeneration, however, the distributions of collagen and PGs content in the decellularized book-shaped enthesis scaffolds from rabbit rotator cuff by SR-FTIR have not been reported.

Methods: Native enthesis tissues (NET) harvested from rabbit rotator cuff were sectioned into cuboid (about 30 mm × 1.2 mm × 10 mm) for decalcification. The decellularized book-shaped enthesis scaffolds and intrinsic ultrastructure were evaluated by histological staining and scanning electron microscopy (SEM), respectively. The distributions of collagen and PGs content in the decellularized book-shaped enthesis scaffolds from rabbit rotator cuff were also measured innovatively by SR-FTIR.

Results: The decellularized book-shaped enthesis scaffolds from rabbit rotator cuff were successfully obtained. Histomorphology and SEM evaluated the effect of decellularization and the structure of extracellular matrix during decellularization. After mechanical testing, the failure load in the NET group showed significantly higher than that in the DEM group (P < 0.05). Meanwhile, the stiffness of the DEM group was significantly lower than the NET group. Furthermore, the distributions of collagen and PGs content in the decellularized book-shaped enthesis scaffolds were decreased obviously after decellularization by SR-FTIR quantitative analysis.

Conclusion: SR-FTIR was applied innovatively to characterize the histological morphology of native enthesis tissues from rabbit rotator cuff. Moreover, this technology can be applied for quantitative mapping of the distribution of collagen and PGs content in the decellularized book-shaped enthesis scaffolds.
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http://dx.doi.org/10.1186/s12891-021-04106-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923620PMC
March 2021

Asbestos-Induced Lung Cancer-Associated SNP rs13383928 Regulates PTH2R Expression in Lung Tissue.

Hum Hered 2021 Feb 5:1-5. Epub 2021 Feb 5.

College of Life Sciences, Shaanxi Normal University, Xi'an, China,

Background: Lung cancer is one of the most common malignant tumors, and asbestos exposure was suggested to contribute to a proportion of lung cancer cases. Previous genome-wide gene-environment interaction analysis reported that rs13383928 was associated with asbestos-related lung cancer. However, the mechanism of this association was still unclear.

Methods: In the present study, we retrieved the genotype data from the 1,000 Genomes Project on single-nucleotide polymorphisms (SNPs) surrounding rs13383928 and analyzed the linkage disequilibrium (LD) pattern of this region. Further functional genomics analyses were performed.

Results: The result indicated that no other SNPs were in LD with rs13383928, suggesting that rs13383928 is the causal one. The following dual luciferase assay disclosed that the T allele of rs13383928 presented significantly higher enhancer activity than G in lung cells, thus verifying that this SNP was functional in the lung. Through chromosome conformation capture, the PTH2R (parathyroid hormone 2 receptor)promoter was identified to interact with the segment surrounding rs13383928. By chromatin immunoprecipitation, it was observed that the region spanning rs13383928 could bind transcription factor FOXJ2 (forkhead box J2).

Conclusion: Our functional genomics evidence supports a link between rs13383928 and asbestos-related lung cancer through regulating PTH2R.
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http://dx.doi.org/10.1159/000513291DOI Listing
February 2021

Cerebrospinal fluid tumor markers predict treatment response in a patient with carcinomatous meningitis.

J Int Med Res 2021 Jan;49(1):300060520987946

Department of Neurology, Hainan Hospital of Chinese PLA General Hospital, Sanya, China.

We report on a 56-year-old female patient diagnosed with carcinomatous meningitis caused by lung cancer. The diagnosis was confirmed by lung computed tomography, enhanced brain magnetic resonance imaging, histopathology, cerebrospinal fluid (CSF) cytology, and serum and CSF tumor markers. Genetic testing detected an epidermal growth factor receptor gene exon 19 deletion. The patient survived for 29 months after systemic treatment with gefitinib, radiotherapy, and chemotherapy. Dynamic monitoring of CSF and serum tumor markers was carried out during the treatment process. We considered that CSF tumor marker levels may have allowed the early diagnosis of meningeal carcinomatosis, and that systemic therapy in the early stage of the disease may prolong survival.
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http://dx.doi.org/10.1177/0300060520987946DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874352PMC
January 2021
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