Publications by authors named "Qian Li"

4,765 Publications

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High-Efficiency Continuous-Luminescence-Controllable Performance and Antithermal Quenching in Bi-Activated Phosphors.

Inorg Chem 2022 Aug 11. Epub 2022 Aug 11.

School of Chemistry and Materials Science, Hunan Agricultural University, Changsha 410128, P. R. China.

Recently, Bi-activated phosphors have been widely researched for phosphor-converted light-emitting diode (pc-LED) applications. Herein, novel full-spectrum ABO:Bi (A = Gd, La; B = Sb, Nb) phosphors with a luminescence-tunable performance were achieved by a chemical substitution strategy. In the LaSbO host material, a new luminescent center was introduced, with Gd replacing La. The photoluminescence (PL) spectra show a large blue shift from 520 to 445 nm, thus achieving regulation from green to blue lights. Moreover, a series of solid solution-phase phosphors LaSbNbO:Bi were prepared by replacing Sb with Nb, and a PL spectral tunability from green (520 nm) to orange-red (592 nm) was realized. Temperature-dependent PL spectra show that LaGdSbO:Bi phosphors have excellent thermal stability. Upon 350 nm excitation, the PL intensity of LaGdSbO:Bi phosphors at 150 °C remained at more than 93% at room temperature. With Gd doping, the thermal stability gradually improved, and LaGdSbO:0.03Bi represents splendid antithermal quenching (135.2% at 150 °C). Finally, a full-visible spectrum for pc-LED with a high color-rendering index (Ra = 94.4) was obtained. These results indicated that chemical substitution is an effective strategy to adjust the PL of Bi, which is of great significance in white-light illumination and accurate plant lighting.
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http://dx.doi.org/10.1021/acs.inorgchem.2c01784DOI Listing
August 2022

Randomized Control Study on Hemoperfusion Combined with Hemodialysis versus Standard Hemodialysis: Effects on Middle-Molecular-Weight Toxins and Uremic Pruritus.

Blood Purif 2022 Aug 11:1-11. Epub 2022 Aug 11.

Department of Nephrology, Shanghai Sixth People's Hospital, Shanghai, China.

Introduction: Classic hemodialysis schedules present inadequate middle-molecular-weight toxin clearance due to limitations of membrane-based separation processes. Accumulation of uremic retention solutes may result in specific symptoms (e.g., pruritus) and may affect clinical outcome and patient's quality of life. Hemoperfusion (HP) is a blood purification modality based on adsorption that can overcome such limitations, and thus, it may be interesting to test the efficacy of at least one session per week of HP combined with hemodialysis. This is a randomized, open-label trial, controlled, multicenter clinical study to investigate the effect of long-term HP combined with hemodialysis on middle-molecular-weight toxins and uremic pruritus in maintenance hemodialysis (MHD) patients.

Methods: 438 MHD patients from 37 HD centers in China with end-stage kidney disease (63.9% males, mean age 51 years) suffering from chronic intractable pruritus were enrolled in the study. Eligible patients were randomized into four groups: low-flux hemodialysis (LFHD), high-flux hemodialysis (HFHD), HP + LFHD, and HP + HFHD at a 1:1:1:1 ratio. Beta-2 microglobulin (β2M) and parathyroid hormone (PTH) were measured at baseline, 3-6, and 12 months. At the same time points, the pruritus score was evaluated. The primary outcome was the reduction of β2M and PTH, while the secondary outcome was the reduction of the pruritus score.

Results: In the two groups HP + LFHD and HP + HFHD, there was a significant decrease of β2M and PTH levels after 12 months compared to the control groups. No significant differences were noted between HP + LFHD and HP + HFHD. Pruritus score reduction was 63% in the HP + LFHD group and 51% in the HP + HFHD group, respectively.

Conclusion: The long-term HP + HD can reduce β2M and PTH levels and improve pruritus in MHD patients independently on the use of high- or low-flux dialyzers, showing that the results are linked to the effect of adsorption.
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http://dx.doi.org/10.1159/000525225DOI Listing
August 2022

Treatment of ulcerative colitis with Wu-Mei-Wan by inhibiting intestinal inflammatory response and repairing damaged intestinal mucosa.

Phytomedicine 2022 Jul 30;105:154362. Epub 2022 Jul 30.

Key Laboratory of Gastrointestinal Diseases and Prescriptions in Shaanxi Province, Xianyang 712046, China; Department of infectious disease, The Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712000, China. Electronic address:

Background: Wu-Mei-Wan (WMW), a traditional Chinese medicine, has been applied in the treatment of gastrointestinal diseases with long-term diarrhea and mucopurulent bloody stool as the main symptoms since ancient times. Studies have shown that WMW inhibits intestinal inflammation, repairs damaged intestinal mucosa, resists colon necrosis, and resists intestinal fibrosis. However, the specific mechanism of action is not yet clear.

Objective: Ulcerative colitis (UC), an intestinal disease with intestinal inflammation and injury as the main pathological manifestations, is one of the high-risk factors for colon cancer. Inhibiting the inflammatory response and promoting colonic epithelial repair are critical to the treatment of UC. However, there is still a lack of remedies with satisfactory curative effects. In this study, the role of WMW in dextran sulfate sodium (DSS)-induced colitis in mice and its related mechanisms are discussed from two aspects: intestinal inflammation and tissue repair.

Methods: DSS was used to induce colitis in mice and the therapeutic effect of WMW was analyzed by disease activity score, histopathological score, colon length measurement, serum cytokine detection, and flow cytometry. Macrophage activation and colonic stem cell proliferation were observed by immunohistochemistry. The expression of critical molecules in macrophage activation and colonic stem cell proliferation signaling pathways in colon tissue was detected with immunohistochemistry, immunofluorescence staining, RT-qPCR, and Western blot.

Results: WMW could significantly alleviate DSS-induced colitis. We showed that WMW could reduce disease activity, reduce pathological scores, limit weight loss, inhibit colon shortening, inhibit inflammatory factor secretion, attenuate inflammatory response, and promote the repair of damaged colonic epithelium. WMW inhibited the activation of colonic macrophages, and its mechanism might be inhibiting the Notch/NF-κB/NLRP3 pathway; WMW promoted the proliferation of colonic stem cells, and its mechanism was associated with the regulation of the Hippo/YAP signaling pathway.

Conclusion: The results of this study suggested that WMW could treat UC via a mechanism that inhibited the intestinal inflammatory response and repaired damaged intestinal mucosa.
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http://dx.doi.org/10.1016/j.phymed.2022.154362DOI Listing
July 2022

Myoglobin diet affected the colonic mucus layer and barrier by increasing the abundance of several beneficial gut bacteria.

Food Funct 2022 Aug 9. Epub 2022 Aug 9.

Key Laboratory of Meat Processing and Quality Control, MOE; Key Laboratory of Meat Processing, MARA; Jiangsu Collaborative Innovation Center of Meat Production, Processing and Quality Control; College of Food Science and Technology, Nanjing Agricultural University, Weigang 1#, 210095, Nanjing, P.R. China.

The study aimed to explore the digestion of a myoglobin diet and its relationship with the gut microbiota and intestinal barrier at two feeding time points. The study indicated that myoglobin diets had a higher α-helix content and lower digestibility. The particle sizes of the digested myoglobin diets were higher but their zeta potential values were lower than those of a casein diet. C57BL/6J mice were fed with a casein diet incorporated with 0, 0.38%, 1.13% and 3.39% myoglobin for 3 and 8 weeks. With the increase of the myoglobin content, the relative abundance of increased significantly. Moreover, the content of short chain fatty acids tended to increase and then decrease as the myoglobin content increased at 3 weeks. Furthermore, the low-myoglobin diet upregulated the gene expression involved in colonic mucin and tight junction proteins by increasing the proportion of beneficial microbiota. However, the high-myoglobin diet had adverse effects.
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http://dx.doi.org/10.1039/d2fo01799gDOI Listing
August 2022

Recombinant LH supplementation improves cumulative live birth rates in the GnRH antagonist protocol: a multicenter retrospective study using a propensity score-matching analysis.

Reprod Biol Endocrinol 2022 Aug 8;20(1):114. Epub 2022 Aug 8.

Reproductive Medicine Center, The Sixth Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510000, China.

Background: Luteinizing hormone (LH) is critical in follicle growth and oocyte maturation. However, the value of recombinant LH (r-LH) supplementation to recombinant follicle stimulating hormone (r-FSH) during controlled ovarian stimulation in the gonadotrophin releasing hormone (GnRH) antagonist regimen is controversial.

Methods: This multicenter retrospective cohort study recruited 899 GnRH antagonist cycles stimulated with r-LH and r-FSH in 3 reproductive centers and matched them to 2652 r-FSH stimulating cycles using propensity score matching (PSM) for potential confounders in a 1:3 ratio. The primary outcome was the cumulative live birth rate (CLBR) per complete cycle.

Results: The baseline characteristics were comparable in the r-FSH/r-LH and r-FSH groups after PSM. The r-FSH/r-LH group achieved a higher CLBR than the r-FSH group (66.95% vs. 61.16%, p = 0.006). R-LH supplementation also resulted in a higher 2-pronuclear embryo rate, usable embryo rate, and live birth rate in both fresh embryo transfer cycles and frozen-thawed embryo transfer (FET) cycles. No significant differences were found in the rate of moderate and severe ovarian hyperstimulation syndrome (OHSS), or cycle cancellation rate in the prevention of OHSS.

Conclusions: R-LH supplementation to r-FSH in the GnRH antagonist protocol was significantly associated with a higher CLBR and live birth rate in fresh and FET cycles, and improved embryo quality without increasing the OHSS rate and cycle cancellation rate.
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http://dx.doi.org/10.1186/s12958-022-00985-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9358814PMC
August 2022

Evaluating quality consistency of cigarette by 3 kinds of quantum fingerprints.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Aug 2;282:121678. Epub 2022 Aug 2.

College of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, PR China. Electronic address:

Cigarette has a history of more than 40 years and once sold well all over the country. The purpose of this paper is to evaluate the quality consistency of 30 batches of cigarettes. Differential scanning calorimetry (DSC) curves, Fourier Transform Infrared (FT-IR) spectra and terahertz time-domain spectra of 30 batches of cigarettes were collected. In this paper, a new quality evaluation method was explored, the obtained fingerprints were quantized, the t-test was used to analyze the correlation between the original fingerprint and the quantum fingerprint. The quantized fingerprints were evaluated by the systematically quantitative fingerprint method (SQFM), the macro qualitative similarity and the macro quantitative similarity were used to reflect the internal differences of the samples. Finally, the antioxidant activities of 30 batches of samples were investigated, and the correlation between quantum fingerprints and IC values were analyzed by Pearson correlation coefficient (r).The result shows that there was no significant difference between the quantum fingerprint and the original fingerprint; SQFM can effectively calculate the similarity of sample curves and classify samples into different grades; the FT-IR fingerprints had a good correlation with the IC value. 30 samples performed a good quality consistency within an acceptable grades range. In addition, quantum fingerprints have broad application prospects in spectral analysis.
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http://dx.doi.org/10.1016/j.saa.2022.121678DOI Listing
August 2022

Epidemic characteristics and transmission risk prediction of brucellosis in Xi'an city, Northwest China.

Front Public Health 2022 22;10:926812. Epub 2022 Jul 22.

Department of Infectious Disease Control and Prevention, Xi'an Center for Disease Prevention and Control, Xi'an, China.

Human brucellosis (HB) has re-emerged in China since the mid-1990s, and exhibited an apparent geographic expansion shifted from the traditional livestock regions to the inland areas of China. It is often neglected in non-traditional epidemic areas, posing a serious threat to public health in big cities. We carried out a retrospective epidemiological study in Xi'an, the largest city in northwestern China. It utilizes long-term surveillance data on HB during 2008-2021 and investigation data during 2014-2021. A total of 1989 HB cases were reported in Xi'an, consisting of 505 local cases, i.e., those located in Xi'an and 1,484 non-local cases, i.e., those located in other cities. Significantly epidemiological heterogeneity was observed between them, mainly owing to differences in the gender, occupation, diagnostic delays, and reporting institutions. Serological investigations suggested that 59 people and 1,822 animals (sheep, cattle, and cows) tested positive for brucellosis from 2014 to 2021, with the annual average seroprevalence rates were 1.38 and 1.54%, respectively. The annual animal seroprevalence rate was positively correlated with the annual incidence of non-local HB cases. Multivariate boosted regression tree models revealed that gross domestic product, population density, length of township roads, number of farms, and nighttime lights substantially contributed to the spatial distribution of local HB. Approximately 7.84 million people inhabited the potential infection risk zones in Xi'an. Our study highlights the reemergence of HB in non-epidemic areas and provides a baseline for large and medium-sized cities to identify regions, where prevention and control efforts should be prioritized in the future.
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http://dx.doi.org/10.3389/fpubh.2022.926812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355750PMC
August 2022

Sparse and risk diversification portfolio selection.

Authors:
Qian Li Wei Zhang

Optim Lett 2022 Jul 31:1-20. Epub 2022 Jul 31.

School of Mathematics, South China University of Technology, Guangzhou, 510640 China.

Portfolio risk management has become more important since some unpredictable factors, such as the 2008 financial crisis and the recent COVID-19 crisis. Although the risk can be actively managed by risk diversification, the high transaction cost and managerial concerns ensue by over diversifying portfolio risk. In this paper, we jointly integrate risk diversification and sparse asset selection into mean-variance portfolio framework, and propose an optimal portfolio selection model labeled as JMV. The weighted piecewise quadratic approximation is considered as a penalty promoting sparsity for the asset selection. The variance associated with the marginal risk regard as another penalty term to diversify the risk. By exposing the feature of JMV, we prove that the KKT point of JMV is the local minimizer if the regularization parameter satisfies a mild condition. To solve this model, we introduce the accelerated proximal gradient (APG) algorithm [Wen in SIAM J. Optim 27:124-145, 2017], which is one of the most efficient first-order large-scale algorithm. Meanwhile, the APG algorithm is linearly convergent to a local minimizer of the JMV model. Furthermore, empirical analysis consistently demonstrate the theoretical results and the superiority of the JMV model.
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http://dx.doi.org/10.1007/s11590-022-01914-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340743PMC
July 2022

Efficacy and safety of intravenous acetaminophen (2 g/day) for reducing opioid consumption in Chinese adults after elective orthopedic surgery: A multicenter randomized controlled trial.

Front Pharmacol 2022 22;13:909572. Epub 2022 Jul 22.

Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

Acetaminophen is an important component of a multimodal analgesia strategy to reduce opioid consumption and pain intensity after an orthopedic surgery. The opioid-sparing efficacy of intravenous acetaminophen has been established at a daily dose of 4 g. However, it is still unclear for the daily dose of 2 g of acetaminophen, which is recommended by the China Food and Drug Administration Center for Drug Evaluation, in terms of its efficacy and safety. This study aimed to evaluate the efficacy and safety of intravenous acetaminophen at a daily dose of 2 g for reducing opioid consumption and pain intensity after orthopedic surgery. In this multicenter, randomized, double-blind, placebo-controlled phase III trial, 235 patients who underwent orthopedic surgery were randomly assigned to receive intravenous acetaminophen 500 mg every 6 h or placebo. Postoperative morphine consumption, pain intensity at rest and during movement, and adverse events were analysed. For the mean (standard deviation) morphine consumption within 24 h after surgery, intravenous acetaminophen was superior to placebo both in the modified intention-to-treat analysis [8.7 (7.7) mg vs. 11.2 (9.2) mg] in the acetaminophen group and the placebo group, respectively. Difference in means: 2.5 mg; 95% confidence interval, 0.25 to 4.61; = 0.030), and in the per-protocol analysis (8.3 (7.0) mg and 11.7 (9.9) mg in the acetaminophen group and the placebo group, respectively. Difference in means: 3.4 mg; 95% confidence interval: 1.05 to 5.77; = 0.005). The two groups did not differ significantly in terms of pain intensity and adverse events. Our results suggest that intravenous acetaminophen at a daily dose of 2 g can reduce morphine consumption by Chinese adults within the first 24 h after orthopedic surgery, but the extent of reduction is not clinically relevant. : [ClinicalTrials.gov], identifier [NCT02811991].
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http://dx.doi.org/10.3389/fphar.2022.909572DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355325PMC
July 2022

Semi-supervised vision transformer with adaptive token sampling for breast cancer classification.

Front Pharmacol 2022 22;13:929755. Epub 2022 Jul 22.

School of Engineering,Penn State Erie, The Behrend College, Erie, PA, United States.

Various imaging techniques combined with machine learning (ML) models have been used to build computer-aided diagnosis (CAD) systems for breast cancer (BC) detection and classification. The rise of deep learning models in recent years, represented by convolutional neural network (CNN) models, has pushed the accuracy of ML-based CAD systems to a new level that is comparable to human experts. Existing studies have explored the usage of a wide spectrum of CNN models for BC detection, and supervised learning has been the mainstream. In this study, we propose a semi-supervised learning framework based on the Vision Transformer (ViT). The ViT is a model that has been validated to outperform CNN models on numerous classification benchmarks but its application in BC detection has been rare. The proposed method offers a custom semi-supervised learning procedure that unifies both supervised and consistency training to enhance the robustness of the model. In addition, the method uses an adaptive token sampling technique that can strategically sample the most significant tokens from the input image, leading to an effective performance gain. We validate our method on two datasets with ultrasound and histopathology images. Results demonstrate that our method can consistently outperform the CNN baselines for both learning tasks. The code repository of the project is available at https://github.com/FeiYee/Breast-area-TWO.
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http://dx.doi.org/10.3389/fphar.2022.929755DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353650PMC
July 2022

Abnormal regional signal in the left cerebellum as a potential neuroimaging biomarker of sudden sensorineural hearing loss.

Front Psychiatry 2022 22;13:967391. Epub 2022 Jul 22.

Department of Otorhinolaryngology, Tianyou Hospital, Affiliated to Wuhan University of Science and Technology, Wuhan, China.

Objective: While prior reports have characterized visible changes in neuroimaging findings in individuals suffering from sudden sensorineural hearing loss (SSNHL), the utility of regional homogeneity (ReHo) as a means of diagnosing SSNHL has yet to be established. The present study was thus conducted to assess ReHo abnormalities in SSNHL patients and to establish whether these abnormalities offer value as a diagnostic neuroimaging biomarker of SSNHL through a support vector machine (SVM) analysis approach.

Methods: Resting-state functional magnetic resonance imaging (rs-fMRI) analyses of 27 SSNHL patients and 27 normal controls were conducted, with the resultant imaging data then being analyzed based on a combination of ReHo and SVM approaches.

Results: Relative to normal control individuals, patients diagnosed with SSNHL exhibited significant reductions in ReHo values in the left cerebellum, bilateral inferior temporal gyrus (ITG), left superior temporal pole (STP), right parahippocampal gyrus (PHG), left posterior cingulum cortex (PCC), and right superior frontal gyrus (SFG). SVM analyses suggested that reduced ReHo values in the left cerebellum were associated with high levels of diagnostic accuracy (96.30%, 52/54), sensitivity (92.59%, 25/27), and specificity (100.00%, 27/27) when distinguishing between SSNHL patients and control individuals.

Conclusion: These data suggest that SSNHL patients exhibit abnormal resting-state neurological activity, with changes in the ReHo of the left cerebellum offering value as a diagnostic neuroimaging biomarker associated with this condition.
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http://dx.doi.org/10.3389/fpsyt.2022.967391DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354585PMC
July 2022

Cooperation of heterotrophic bacteria enables stronger resilience of halophilic assimilation biosystem than nitrification system under long-term stagnation.

Sci Total Environ 2022 Aug 3:157806. Epub 2022 Aug 3.

School of Civil Engineering, Shandong University, Jinan, Shandong 250002, China. Electronic address:

Long-term stagnation of biosystems (with no or very little wastewater) owing to seasonal downtime or failure maintenance brings great challenges to the performance recovery after system restart. In particular, the reduction of microbial activity and change of dissolved organic matter (DOM) affect the effluent quality and subsequent treatment procedures. Monitoring the dynamics and resilience of biosystems after long-term stagnation is important to formulate targeted countermeasures for system stability. However, the influence of long-term stagnation on autotrophic nitrification (AN) and heterotrophic assimilation (HA) biosystems has not been systematically explored. Here, we used halophilic AN and HA systems to study the stability and resilience of two nitrogen removal consortia after long-term stagnation. The results showed that 97.5 % and 93 % of ammonium and 47.0 % and 90.1 % of total nitrogen were removed using the halophilic AN and HA systems, respectively, in the stable period. After four weeks of stagnation, the HA system showed stronger resilience than AN system, in terms of faster recovery of treatment performance, and less fluctuations in sludge settleability and extracellular polymeric substances. In addition, after the stagnation period, the DOM of AN system was rich in low-molecular refractory humic acid, whereas that of HA system was rich in high-molecular proteins. The stagnation period led to the replacement of the dominant heterotrophic functional microorganisms, Paracoccus and Halomonas, with Muricauda and Marinobacterium in the HA system. The microbial network results revealed that the cooperation of heterotrophic bacteria enables stronger resilience of the HA system from prolonged stagnation than the AN system. In addition, the nitrogen removal efficiency, protein to polysaccharide ratio of EPS and fluorescence intensity of DOM were significantly correlated with the microbial community composition. These results suggest that AN system has greater risks in terms of treatment performance and sludge stability than the system after long-term stagnation.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157806DOI Listing
August 2022

Stacked dilated convolutions and asymmetric architecture for U-Net-based medical image segmentation.

Comput Biol Med 2022 Jul 21;148:105891. Epub 2022 Jul 21.

Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, 02114, MA, USA.

Deep learning has been widely utilized for medical image segmentation. The most commonly used U-Net and its variants often share two common characteristics but lack solid evidence for the effectiveness. First, each block (i.e., consecutive convolutions of feature maps of the same resolution) outputs feature maps from the last convolution, limiting the variety of the receptive fields. Second, the network has a symmetric structure where the encoder and the decoder paths have similar numbers of channels. We explored two novel revisions: a stacked dilated operation that outputs feature maps from multi-scale receptive fields to replace the consecutive convolutions; an asymmetric architecture with fewer channels in the decoder path. Two novel models were developed: U-Net using the stacked dilated operation (SDU-Net) and asymmetric SDU-Net (ASDU-Net). We used both publicly available and private datasets to assess the efficacy of the proposed models. Extensive experiments confirmed SDU-Net outperformed or achieved performance similar to the state-of-the-art while using fewer parameters (40% of U-Net). ASDU-Net further reduced the model parameters to 20% of U-Net with performance comparable to SDU-Net. In conclusion, the stacked dilated operation and the asymmetric structure are promising for improving the performance of U-Net and its variants.
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http://dx.doi.org/10.1016/j.compbiomed.2022.105891DOI Listing
July 2022

Phytochrome Interacting Factor regulates stomatal aperture by coordinating red light and abscisic acid.

Plant Cell 2022 Aug 5. Epub 2022 Aug 5.

Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, China.

Stomata are crucial valves coordinating the fixation of carbon dioxide by photosynthesis and water loss through leaf transpiration. Phytochrome interacting factors (PIFs) are negative regulators of red light responses that belong to the basic helix-loop-helix (bHLH) family of transcription factors. Here, we show that the rice (Oryza sativa) PIF family gene OsPIL15 acts as a negative regulator of stomatal aperture to control transpiration in rice. OsPIL15 reduces stomatal aperture by activating rice ABSCISIC ACID INSENSITIVE 5 (OsABI5), which encodes a critical positive regulator of abscisic acid (ABA) signaling in rice. Moreover, OsPIL15 interacts with the NIGT1/HRS1/HHO family transcription factor rice HRS1 HOMOLOG 3 (OsHHO3) to possibly enhance the regulation of stomatal aperture. Notably, we discovered that the maize (Zea mays) PIF family genes ZmPIF1 and ZmPIF3, which are homologous to OsPIL15, are also involved in the regulation of stomatal aperture in maize, indicating that PIF-mediated regulation of stomatal aperture may be conserved in the plant lineage. Our findings explain the molecular mechanism by which PIFs play a role in red-light-mediated stomatal opening, and demonstrate that PIFs regulate stomatal aperture by coordinating the red light and ABA signaling pathways.
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http://dx.doi.org/10.1093/plcell/koac244DOI Listing
August 2022

Shudi Erzi San relieves ovary aging in laying hens.

Poult Sci 2022 Jun 28;101(9):102033. Epub 2022 Jun 28.

College of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China. Electronic address:

Poultry meat and eggs are a primary source of animal protein. To meet the market needs, high yield laying hens are reared continuously, resulting in quick ovary aging. Thus, we investigated the anti-aging effects of Shudi Erzi San (SES) on laying hens. Sixty 300-day-old laying hens were divided into 2 experimental groups and a control group. The control group was fed on a basic diet, which was supplemented with 1% and 2% SES for experimental groups I and II, respectively. Egg quality and changes in serum hormones and blood-biochemical indicators of laying hens were determined. The rate of egg production was significantly higher in group Ⅱ than in both the control and group Ⅰ by 9.29 and 8.22 percentage points, respectively (P < 0.05). Eggshell strength of groups Ⅰ and Ⅱ were significantly higher than that of the control group (P < 0.01). Albumen height and Haugh Units of group Ⅱ were significantly higher than those of the control (P < 0.05). Serum levels of follicle stimulating hormone and estradiol in group Ⅱ were significantly higher than those of both the control and group Ⅰ (P < 0.05), whereas groups Ⅰ and Ⅱ had significantly higher serum levels of luteinizing hormone than the control (P < 0.05). Levels of superoxide dismutase (SOD) did not significantly differ between the control and group Ⅰ (P > 0.05), but SOD and malondialdehyde (MDA) levels in group Ⅱ were significantly higher and lower, respectively (P < 0.05) when compared to the control. Compared with the control, uric acid levels in groups Ⅰ and Ⅱ were significantly lower (P < 0.05), as was urea nitrogen in group Ⅱ (P < 0.05). Transcriptome and KEGG pathway analysis of ovarian tissues of laying hens showed a significant immune related signal pathway as the possible main regulator of a lysosome related signal pathway. Thus, supplementing chicken feed with SES improves egg production and quality and alleviates ovarian decline in laying hens.
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http://dx.doi.org/10.1016/j.psj.2022.102033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356177PMC
June 2022

Highly efficient ethanol vapour detection using g-CN/ZnO micro flower-like heterostructural composites.

RSC Adv 2022 Jul 18;12(32):20618-20627. Epub 2022 Jul 18.

Anhui Provincial Engineering Laboratory of Silicon-based Materials, School of Material and Chemical Engineering, Bengbu University Bengbu 233030 People's Republic of China

This work proposes precursor pyrolysis, ultrasonic exfoliation and hydrothermal methods as well as high-temperature calcination strategies to fabricate heterostructured g-CN/ZnO composites with excellent ethanol vapour sensing properties. The structure, composition and morphology of the as-prepared g-CN/ZnO composites were characterized using X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Then, the sensing properties of the g-CN/ZnO composites for ethanol (CHOH) were studied, and g-CN doping with different mass ratios was used to control the gas-sensing properties of the composites. Compared with pure ZnO and g-CN, the performance of g-CN with 1% doping content is the best, and the gas sensing activity of the 1% g-CN/ZnO composite is greatly improved at the optimal working temperature (280 °C). The response to 100 ppm ethanol reaches 81.4, which is 3.7 times that of the pure ZnO-based sensor under the same conditions. In addition, the sensor has good selectivity as well as fast response and recovery speeds (24 s and 63 s, respectively). Finally, a reasonable gas sensing enhancement mechanism is proposed, and it is believed that the constructed g-CN/ZnO micro flower-like heterostructure and the distinct positions of the valence and conduction bands of ZnO and g-CN lead to the obtained sensor exhibiting a large specific surface area and increased conductivity, thereby improving the g-CN/ZnO-based sensor sensing performance.
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http://dx.doi.org/10.1039/d2ra02609kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9289812PMC
July 2022

Long Non-Coding RNA LINC00052 Targets miR-548p/Notch2/Pyk2 to Modulate Tumor Budding and Metastasis of Human Breast Cancer.

Biochem Genet 2022 Aug 2. Epub 2022 Aug 2.

Department of Breast Surgery, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital of Sun Yat-Sen University, No. 26 Erheng Road, Yuancun, Tianhe District, Guangzhou, 510655, Guangdong, China.

Abnormal expression of long non-coding RNAs (lncRNAs) is involved in many pathological processes of cancers. However, the role of lncRNA LINC00052 in breast cancer progression is still unclear. Here, LINC00052 expression was detected by in situ hybridization and quantitative real-time PCR assays. Cell Counting Kit-8, wound healing, and transwell assays were used to investigate changes in the proliferation, migration, and invasion of breast cancer cells. MiR-548p was found associated with LINC00052 or Notch2 by RNA pull-down, dual-luciferase reporter, and qRT-PCR assays. The effect of LINC00052 on lung metastasis was explored through in vivo experiments. High LINC00052 expression was observed in breast cancer tissues and cells. LINC00052 silencing inhibited the proliferation, migration, and invasion of MCF7 cells, and LINC00052 overexpression produced the opposite results. MiR-548p, a target gene of LINC00052, partially rescued the effects of LINC00052 on proliferation, migration, and invasion of MCF7. Notch2 was the target of miR-548p and LINC00052 could promote Notch2 expression. Moreover, the phosphorylation of proline-rich tyrosine kinase 2 (Pyk2), a downstream factor of Notch2, was increased by LINC00052, and a Pyk2 mutant could inhibit the cell migration and invasion induced by LINC00052 overexpression in MDA-MB-468 cells, which was similar to the function of the miR-548p mimic. We further demonstrated that LINC00052 exacerbated the metastases of breast cancer cells in vivo. Our research demonstrated that LINC00052 is highly expressed in breast cancer and promotes breast cancer proliferation, migration, and invasion via the miR-548p/Notch2/Pyk2 axis. LINC00052 could serve as a potential therapeutic target for breast cancer.
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http://dx.doi.org/10.1007/s10528-022-10255-yDOI Listing
August 2022

CircIPO7 Promotes Nasopharyngeal Carcinoma Metastasis and Cisplatin Chemoresistance by Facilitating YBX1 Nuclear Localization.

Clin Cancer Res 2022 Aug 2. Epub 2022 Aug 2.

Sun Yat-sen University Cancer Center, Guangzhou, China.

Purpose: Cisplatin-based chemotherapy effectively improves the distant-metastasis control in nasopharyngeal carcinoma (NPC), but approximately 30% of patients develop treatment failure due to chemoresistance. However, the underlying mechanisms remain poorly understood.

Experimental Design: CircRNA sequencing data were used to identify metastasis-specific circRNAs and the expression of circIPO7 was validated in NPC tissues as well as NPC cell lines by qRT-PCR. The whole transcriptional profile upon circIPO7 knockdown was applied to explore the biological function and regulatory mechanism, which were further confirmed by in vitro and in vivo metastasis/chemosensitivity assays. We also evaluated the value of circIPO7 expression in predicting NPC metastasis and cisplatin chemoresistance by analyzing a cohort of 183 NPC patients.

Results: In the current study, circIPO7, a novel circular RNA (circRNA), is found to be specifically overexpressed in NPC patients with distant metastasis. Knockdown of circIPO7 in NPC cells suppresses their metastasis and increases sensitivity to cisplatin treatment in vitro and in vivo. Mechanistically, circIPO7 binds to Y-box binding protein-1 (YBX1) protein in the cytoplasm and facilitates its phosphorylation at serine 102 (p-YBX1S102) by the kinase AKT, which further promotes YBX1 nuclear translocation and activates FGFR1, TNC, and NTRK1 transcription. Clinically, higher circIPO7 expression indicates unfavorable distant metastasis-free survival in NPC patients given cisplatin-based chemotherapy.

Conclusions: Altogether, this study identifies oncogenic circIPO7 as a prognostic marker after cisplatin-based chemotherapy and as a potential therapeutic target for overcoming metastasis and chemoresistance in NPC.
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http://dx.doi.org/10.1158/1078-0432.CCR-22-0991DOI Listing
August 2022

Effect of Surface Heterogeneities on Interactions between Air Bubbles and Heterogeneous Surfaces.

Langmuir 2022 Aug 2. Epub 2022 Aug 2.

School of Resource & Environment and Safety Engineering, University of South China, Hengyang 421001, China.

The effects of surface heterogeneities on bubble-particle interactions have received little attention although heterogeneities are common for varieties of substance surfaces. In this work, heterogeneous surfaces consisting of discrete hydrophilic dots on a hydrophobic background were fabricated. The interactions between air bubbles and heterogeneous surfaces with different hydrophilic area fractions were investigated using a high-sensitivity microbalance coupled with a high-speed video camera. It was found that the snap-in, maximum adhesion, and pull-off forces increased as the hydrophilic area fraction decreased. These experimental results were compared with the calculated interaction forces. The comparison between experimental results and the calculated interaction forces showed that the normalized contact line length (δ) should be considered in the calculation of the snap-in force, and its value was between 1 and the δ value corresponding to the maximum pinning strength. In contrast, δ = 1 is more appropriate for the calculation of maximum adhesion force, indicating that the corrugations in the three-phase contact line could be neglected. These findings demonstrate that discrete hydrophilic defects make bubble-surface attachment difficult but have nearly no effect on bubble-surface detachment. Better understanding of the interactions between air bubbles and heterogeneous surfaces potential offers a new thought to control the bubble-particle interactions using appropriately design of particle surfaces.
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http://dx.doi.org/10.1021/acs.langmuir.2c01126DOI Listing
August 2022

DNA-PAINT Super-Resolution Imaging for Characterization of Nucleic Acid Nanostructures.

Chempluschem 2022 Aug;87(8):e202200127

School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, Institute of Translational Medicine, Shanghai Jiao Tong University, Dongchuan Road 800, Shanghai, 200240, P. R. China.

Numerous nucleic acid nanostructures of unique addressability and programmability have been fabricated for emerging applications. Structural characterization with atomic force microscopy and electron microscopy can provide information on the structural morphology and precision of these nanostructures. However, either structural information of native nucleic acid nanostructures in hydrated environment or the availability of addressable sites on these nanostructures could not be determined. Alternatively, DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) enables direct optical visualization of nucleic acid nanostructures in native forms, as well as evaluation of the accessibility of addressable sites on them. In this Review, the working principle of DNA-PAINT is introduced, followed by the summary on advances of DNA-PAINT characterization of various nucleic acid nanostructures. Finally, the current challenges and prospects for DNA-PAINT characterization are presented. We envision DNA-PAINT to be a potent characterization tool for functional nanomaterials.
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http://dx.doi.org/10.1002/cplu.202200127DOI Listing
August 2022

Long-Term Clinical Outcomes After Percutaneous Coronary Intervention With Drug-Coated Balloon-Only Strategy in Lesions of Large Coronary Arteries.

Front Cardiovasc Med 2022 14;9:882303. Epub 2022 Jul 14.

Department of Cardiology, Xuzhou Third People's Hospital, Xuzhou Medical University, Xuzhou, China.

Background And Objectives: Use of drug-coated balloon (DCB)-only strategy for revascularization of native large coronary artery lesions is on the rise. The long-term efficacy of this approach for bifurcation and non-bifurcation lesions remains unknown. We aim to assess the long-term clinical outcomes of DCB-only strategy for the treatment of bifurcation and non-bifurcation lesions in large coronary arteries.

Methods: This multicenter, prospective, observational study enrolled 119 patients with coronary lesions in vessels ≥2.75 mm. The primary end point was the rate of clinically driven target lesion failure (TLF), a composite of cardiac death, target vessel myocardial infarction, and clinically driven target lesion revascularization (TLR). Patients were followed up for a median of 2 years.

Results: Of 119 patients with 138 lesions, 66 patients (75 lesions) had bifurcation and 53 patients (63 lesions) had non-bifurcation lesions. Average reference vessel diameter was 3.1 ± 0.3 mm, and there was no difference in bifurcation and non-bifurcation group (3.0 ± 0.3 vs. 3.1 ± 0.3mm; = 0.27). At 2-year follow-up, the TLF occurred in five (4.2%), TLR in four (3.4%), and target vessel revascularization (TVR) in five (4.2%) cases. The frequency of TLR and TVR was higher in the non-bifurcation group ( = 0.04 and 0.02, respectively), but there were no differences in TLF between the two groups ( = 0.17). The cumulative incidence of TLF (Kaplan-Meier estimates) was also not different in the two groups (log-rank = 0.11).

Conclusion: DCB-only strategy for lesions in large coronary arteries appears to be safe and effective for both bifurcation and non-bifurcation lesions. Further randomized clinical trials are warranted to confirm the value of DCB-only strategy in bifurcation lesions of large vessels.
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http://dx.doi.org/10.3389/fcvm.2022.882303DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329593PMC
July 2022

The Diagnostic Value of Mitochondrial Mass of Peripheral T Lymphocytes in Early Sepsis.

Front Public Health 2022 14;10:928306. Epub 2022 Jul 14.

Emergency and Critical Care Center, Department of Emergency Medicine, Zhejiang Provincial People's Hospital (Affiliated People's Hospital, Hangzhou Medical College), Hangzhou, China.

Background: Studies have shown that lymphocyte dysfunction can occur during the early stages of sepsis and that cell dysfunction is associated with mitochondrial dysfunction. Therefore, quantifying the mitochondrial function of lymphocytes in patients with sepsis could be valuable for the early diagnosis of sepsis.

Methods: Seventy-nine patients hospitalized from September 2020 to September 2021 with Sepsis-3 were retrospectively analyzed and subsequently compared with those without sepsis.

Results: Univariate analysis showed statistical differences between the data of the two groups regarding age, neutrophil/lymphocyte, procalcitonin (PCT), C-reactive protein, total bilirubin, serum creatinine, type B natriuretic peptide, albumin, prothrombin time, activated partial thromboplastin time, lactic acid, single-cell mitochondrial mass (SCMM)-CD3, SCMM-CD4, SCMM-CD8, and Acute Physiology and Chronic Health Evaluation II score ( < 0.05). Multivariate logistic regression analysis performed on the indicators mentioned above demonstrated a statistical difference in PCT, lactic acid, SCMM-CD4, and SCMM-CD8 levels between the two groups ( < 0.05). The receiver operating characteristic curves of five models were subsequently compared [area under the curve: 0.740 (PCT) vs. 0.933 (SCMM-CD4) vs. 0.881 (SCMM-CD8) vs. 0.961 (PCT + SCMM-CD4) vs. 0.915 (PCT+SCMM-CD8), < 0.001].

Conclusion: SCMM-CD4 was shown to be a better diagnostic biomarker of early sepsis when compared with the traditional biomarker, PCT. Furthermore, the value of the combination of PCT and SCMM-CD4 in the diagnosis of early sepsis was better than that of SCMM-CD4 alone.
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http://dx.doi.org/10.3389/fpubh.2022.928306DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330378PMC
August 2022

5-Aza-dC promotes T-cell acute lymphoblastic leukemia cell invasion via downregulation of DNMT1 and upregulation of MMP-2 and MMP-9.

Exp Hematol 2022 Jul 28. Epub 2022 Jul 28.

Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou 350004, P. R. China. Electronic address:

5-aza-2'-deoxycytidine (5-Aza-dC) is a demethylation agent known to deplete DNA methyltransferases (DNMTs) in leukemia cancer cells and can restore the expression of their target genes in Jurkat cells. This study intended to discern the potential effect of 5-Aza-dC on the invasion of T-ALL cells in acute lymphoblastic leukemia (ALL). The role of MMP-2, MMP-9 and DNMT1 in cell invasion was determined using loss- and gain-of-function investigations in Jurkat- and Sup-T1-R cells. A nude mouse model of ALL was established for further exploration of their roles in vivo. MMP-2 and MMP-9 presented high expression and low DNA methylation levels in 5-Aza-dC-resistant T-ALL cells. DNMT1 was poorly expressed in 5-Aza-dC-resistant T-ALL cells and exhibited decreased enrichment in the promoter region of MMP-2 and MMP-9. Silencing of MMP-2 and MMP-9 or DNMT1 overexpression reduced T-ALL cell invasion. After treatment of Sup-T1 cells with 5-Aza-dC, MMP-2 and MMP-9 presented with reduced DNA methylation levels but increased expression, and DNMT1 expression was identified to be suppressed. Further, in vivo assays demonstrated that DNMT1 alleviated T-ALL by reducing the expression of MMP-2 and MMP-9 in vivo. All in all, 5-Aza-dC activates MMP-2 and MMP-9 expression by reducing DNMT1-dependent DNA methylation levels, and hence promotes the invasion of T-ALL cells.
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http://dx.doi.org/10.1016/j.exphem.2022.07.301DOI Listing
July 2022

Modelling the impact of universal influenza vaccines on seasonal influenza with different subtypes.

Epidemiol Infect 2021 Nov 2;149:e253. Epub 2021 Nov 2.

China-Australia Joint Research Center for Infectious Diseases, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi710061, China.

Several candidates of universal influenza vaccine (UIV) have entered phase III clinical trials, which are expected to improve the willingness and coverage of the population substantially. The impact of UIV on the seasonal influenza epidemic in low influenza vaccination coverage regions like China remains unclear. We proposed a new compartmental model involving the transmission of different influenza subtypes to evaluate the effects of UIV. We calibrated the model by weekly surveillance data of influenza in Xi'an City, Shaanxi Province, China, during 2010/11-2018/19 influenza seasons. We calculated the percentage of averted infections under 2-month (September to October) and 6-month (September to the next February) vaccination patterns with varied UIV effectiveness and coverage in each influenza season, compared with no UIV scenario. A total of 195 766 influenza-like illness (ILI) cases were reported during the nine influenza seasons (2010/11-2018/19), of which the highest ILI cases were among age group 0-4 (59.60%) years old, followed by 5-14 (25.22%), 25-59 (8.19%), 15-24 (3.75%) and ⩾60 (3.37%) years old. The influenza-positive rate for all age groups among ILI cases was 17.51%, which is highest among 5-14 (23.75%) age group and followed by 25-59 (16.44%), 15-24 (16.42%), 0-4 (14.66%) and ⩾60 (13.98%) age groups, respectively. Our model showed that UIV might greatly avert influenza infections irrespective of subtypes in each influenza season. For example, in the 2018/19 influenza season, 2-month vaccination pattern with low UIV effectiveness (50%) and coverage (10%), and high UIV effectiveness (75%) and coverage (30%) could avert 41.6% (95% CI 27.8-55.4%) and 83.4% (80.9-85.9%) of influenza infections, respectively; 6-month vaccination pattern with low and high UIV effectiveness and coverage could avert 32.0% (15.9-48.2%) and 74.2% (69.7-78.7%) of influenza infections, respectively. It would need 11.4% (7.9-15.0%) of coverage to reduce half of the influenza infections for 2-month vaccination pattern with low UIV effectiveness and 8.5% (5.0-11.2%) of coverage with high UIV effectiveness, while it would need 15.5% (8.9-20.7%) of coverage for 6-month vaccination pattern with low UIV effectiveness and 11.2% (6.5-15.0%) of coverage with high UIV effectiveness. We conclude that UIV could significantly reduce the influenza infections even for low UIV effectiveness and coverage. The 2-month vaccination pattern could avert more influenza infections than the 6-month vaccination pattern irrespective of influenza subtype and UIV effectiveness and coverage.
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http://dx.doi.org/10.1017/S0950268821002284DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8697312PMC
November 2021

Engineering the β-Fructofuranosidase Fru6 with Promoted Transfructosylating Capacity for Fructooligosaccharide Production.

J Agric Food Chem 2022 Aug 28;70(31):9694-9702. Epub 2022 Jul 28.

School of Pharmaceutical Sciences, Nanjing Tech University, 30 Puzhunan Road, Jiangbei New Area, Nanjing211800, China.

Levan-type fructooligosaccharides (FOS) exhibit enhanced health-promoting prebiotic effects on gut microbiota. The wild type (WT) of β-fructofuranosidase Fru6 could mainly yield 6-ketose. Semirational design and mutagenesis of Fru6 were exploited to promote the transfructosylating capacity for FOS. The promising variants not only improved the formation of 6-kestose but also newly produced tetrasaccharides of 6,6-nystose and 1,6-nystose (a new type of FOS), and combinatorial mutation boosted the production of 6-kestose and tetrasaccharides (39.9 g/L 6,6-nystose and 4.6 g/L 1,6-nystose). Molecular docking and molecular dynamics (MD) simulation confirmed that the mutated positions reshaped the pocket of Fru6 to accommodate bulky 6-kestose in a reactive conformation with better accessibility for tetrasaccharides formation. Using favored conditions, the variant S165A/H357A could yield 6-kestose up to 335 g/L, and tetrasaccharides (6,6-nystose and 1,6-nystose) reached a high level of 121.1 g/L (134.5 times of the mutant S423A). The β-(2,6)-linked FOS may show the potential application for the prebiotic ingredients.
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http://dx.doi.org/10.1021/acs.jafc.2c03981DOI Listing
August 2022

The Dynamic Accumulation Rules of Chemical Components in Different Medicinal Parts of by GC-MS.

Molecules 2022 Jul 20;27(14). Epub 2022 Jul 20.

State Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.

The chemical components and medicinal properties of different medicinal parts of are often used as medicine after being divided into the head, body and tail of . In this study, the chemical components of different medicinal parts in different periods were analyzed by GC-MS for the first time, and the differences of the accumulation rules of chemical components in different medicinal parts of were obtained. This study demonstrated that the differences of composition accumulation in different medicinal parts of were mainly reflected in the types and relative contents of compounds. The study found that the number of compounds in different medicinal parts of in each period were different and the change rules of the same compound in different medicinal parts were also different. The number of compounds in the tail of was the least in April, and the largest in October. The content of ligustilide in the body of was higher in April and was the highest in the tail in October. The relative content of butylidenephthalide in the head was the highest in October. The relative contents of senkyunolide A and butylphthalide in the head were decreased in October, while the contents in the body and tail increased, indicating that the compounds that accumulate in the head may transfer to the body and tail in later stages of growth. This study clarified the differences in the accumulation of chemical components in different medicinal parts of , which could provide a theoretical basis for the reasons for the differences of chemical components in the different medicinal parts.
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http://dx.doi.org/10.3390/molecules27144617DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317102PMC
July 2022

New Evidence of Tiger Subspecies Differentiation and Environmental Adaptation: Comparison of the Whole Genomes of the Amur Tiger and the South China Tiger.

Animals (Basel) 2022 Jul 16;12(14). Epub 2022 Jul 16.

College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, China.

is a top predator that maintains the integrity of forest ecosystems and is an integral part of biodiversity. No more than 400 Amur tigers () are left in the wild, whereas the South China tiger () is thought to be extinct in the wild, and molecular biology has been widely used in conservation and management. In this study, the genetic information of Amur tigers and South China tigers was studied by whole-genome sequencing (WGS). A total of 647 Gb of high-quality clean data was obtained. There were 6.3 million high-quality single-nucleotide polymorphisms (SNPs), among which most (66.3%) were located in intergenic regions, with an average of 31.72% located in coding sequences. There were 1.73 million insertion-deletions (InDels), among which there were 2438 InDels (0.10%) in the coding region, and 270 thousand copy number variations (CNVs). Significant genetic differences were found between the Amur tiger and the South China tiger based on a principal component analysis and phylogenetic tree. The linkage disequilibrium analysis showed that the linkage disequilibrium attenuation distance of the South China tiger and the Amur tiger was almost the same, whereas the r of the South China tiger was 0.6, and the r of the Amur tiger was 0.4. We identified functional genes and regulatory pathways related to reproduction, disease, predation, and metabolism and characterized functional genes related to survival in the wild, such as smell, vision, muscle, and predatory ability. The data also provide new evidence for the adaptation of Amur tigers to cold environments. is involved in temperature regulation in a cold climate. and regulate body temperature to keep it constant. Our results can provide genetic support for precise interspecies conservation and management planning in the future.
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http://dx.doi.org/10.3390/ani12141817DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312029PMC
July 2022

Drug-free in vitro activation combined with 3D-bioprinted adipose-derived stem cells restores ovarian function of rats with premature ovarian insufficiency.

Stem Cell Res Ther 2022 07 26;13(1):347. Epub 2022 Jul 26.

Department of Obstetrics and Gynecology, The Second Hospital of Hebei Medical University, 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.

Background: Emerging drug-free in vitro activation (IVA) technique enables patients with premature ovarian insufficiency (POI) to restore ovarian function and conceive their own genetic offspring. However, various issues have greatly restricted its clinical application. Transplantation of adipose-derived stem cells (ADSCs) has promising roles in restoring ovarian function of rats with POI, but insufficient retention has greatly hampered their efficiency. Here, we designed a 3D-bioprinted engineering ovary composed of drug-free IVA and ADSCs, which may prolong the retention of ADSCs and construct an early vascular microenvironment, thus compensating for the disadvantages of drug-free IVA to some extent and ameliorating impaired ovarian function in the POI rats.

Methods: After intraperitoneal injection of cyclophosphamide, the POI model rats were randomized into 5 groups: (1) POI group; (2) ovarian fragments group; (3) 3D scaffold combined with ovarian fragments group; (4) ovarian fragments combined with ADSCs group; (5) 3D scaffold with ADSCs combined with ovarian fragments as 3D-bioprinted engineering ovary group. Normal rats were identified as the control group. The localization of CM-Dil-labeled ADSCs and co-localization with CD31 were observed to examine the distribution and underlying mechanism of differentiation. Histomorphological and immunohistochemical analyses were performed to calculate follicle number and assess proliferation and apoptosis of granulosa cells (GCs). Immunofluorescence staining was used to evaluate angiogenesis. Hormone levels were measured to evaluate the restoration of endocrine axis. Western blot analysis and RT-PCR were conducted to explore the potential mechanism.

Results: CM-Dil-labeled ADSCs were distributed in the interstitium of ovaries and had significantly higher retention in the 3D-bioprinted engineering ovary group. Several regions of the co-staining for CM-Dil and CD31 were in the area of vascular endothelial cells. Meanwhile, the follicle counts, GCs proliferation, neoangiogenesis, and hormone levels were significantly improved in the 3D-bioprinted engineering ovary group, as compared with other groups. Furthermore, the ovarian function was ameliorated and angiogenesis was promoted through regulating the PI3K/AKT pathway.

Conclusion: Our results suggested that 3D-bioprinted engineering ovary had great potential for restoring impaired ovarian function of rats with POI, which could compensate for the disadvantages of drug-free IVA to some extent.
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http://dx.doi.org/10.1186/s13287-022-03035-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9327214PMC
July 2022

Research on Gut Microbiota-Derived Secondary Bile Acids in Cancer Progression.

Authors:
Rong Yang Li Qian

Integr Cancer Ther 2022 Jan-Dec;21:15347354221114100

Medical College, Yangzhou University, Yangzhou, China.

The interaction between gut microbiota-derived metabolites and the body plays a significant role in the occurrence and development of cancer. Secondary bile acids (BAs) are the important products produced from gut microbial fermentation of primary BAs, mainly deoxycholic acid (DCA) and lithocholic acid (LCA). In the gut, they can influence the structure of the microbial communities. Several studies have demonstrated that secondary BAs, as signaling molecules, can activate a variety of signaling pathways. They can inhibit the apoptosis of cancer cells, induce the progression of cancer cell cycles, enhance the ability of metastasis and invasion of cancer cells, and promote the transformation of cells into cancer stem cells (CSCs). Moreover, secondary BAs promote cancer by regulating the function of immune cells. Therefore, targeted manipulation of gut microbial and secondary BAs has the potential to be developed as for treatment and prevention of various cancers.
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http://dx.doi.org/10.1177/15347354221114100DOI Listing
July 2022

Functional analysis, diversity, and distribution of carbendazim hydrolases MheI and CbmA, responsible for the initial step in carbendazim degradation.

Environ Microbiol 2022 Jul 26. Epub 2022 Jul 26.

Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, PR China.

Strains Rhodococcus qingshengii djl-6 and Rhodococcus jialingiae djl-6-2 both harbor the typical carbendazim degradation pathway with the hydrolysis of carbendazim to 2-aminobenzimidazole (2-AB) as the initial step. However, the enzymes involved in this process are still unknown. In this study, the previous reported carbendazim hydrolase MheI was found in strain djl-6, but not in strain djl-6-2, then another carbendazim hydrolase CbmA was obtained by a four-step purification strategy from strain djl-6-2. CbmA was classified as a member of the amidase signature superfamily with conserved catalytic site residues Ser157, Ser181, and Lys82, while MheI was classified as a member of the Abhydrolase superfamily with conserved catalytic site residues Ser77 and His224. The catalytic efficiency (k /K ) of MheI (24.0-27.9 μM min ) was 200 times more than that of CbmA (0.032-0.21 μM min ). The mheI gene (plasmid encoded) was highly conserved (> 99% identity) in the strains from different bacterial genera and its plasmid encoded flanked by mobile genetic elements. The cmbA gene was highly conserved only in strains of the genus Rhodococcus and it was chromosomally encoded. Overall, the function, diversity, and distribution of carbendazim hydrolases MheI and CbmA will provide insights into the microbial degradation of carbendazim.
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http://dx.doi.org/10.1111/1462-2920.16139DOI Listing
July 2022
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