Publications by authors named "Periyasamy Kumar"

9 Publications

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Survey on the impact of COVID19 in patients on immunosuppression for ocular and orbital inflammatory disorders.

Eur J Ophthalmol 2021 Apr 8:11206721211008039. Epub 2021 Apr 8.

Department of Ophthalmology, University Hospitals of Leicester, Leicester Royal Infirmary, Leicester, UK.

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http://dx.doi.org/10.1177/11206721211008039DOI Listing
April 2021

An Atypical Presentation of Sympathetic Ophthalmia in an Intact Globe Following Mechanical Fall: A Case Report and Literature Review.

Vision (Basel) 2021 Feb 21;5(1). Epub 2021 Feb 21.

Department of Ophthalmology, University Hospitals of Leicester NHS Trust, Infirmary Square, Leicester LE1 5WW, UK.

Purpose: To describe an atypical case of sympathetic ophthalmia presenting after blunt trauma causing disinsertion of the iris in an intact globe.

Methods: Case report.

Results: A 71-year-old lady presented to the Emergency Department following a mechanical fall. On examination, she was noted to have periocular haematoma, subconjunctival haemorrhage, hyphaema, and vitreous haemorrhage in the left eye, but there was no evidence of globe rupture. The presenting visual acuity was 6/18. As the hyphaema and vitreous haemorrhage settled, a complete loss of the iris was noted with normal fundus. She was re-admitted a month later under the medical team with urinary tract infection and reduced vision in both eyes. On examination, there was mild conjunctival injection, keratic precipitates, anterior chamber flare, 180-degree posterior synechiae, and vitritis with no fundal view of the right eye. She was diagnosed with sympathetic ophthalmia and was treated with topical and systemic corticosteroid. Her vision improved gradually with treatment and was stable at 6/6 on the right (sympathising) eye and 6/9 on the left (excited) eye at final follow-up.

Conclusion: Sympathetic ophthalmia may result from non-penetrating ocular trauma. Comprehensive history of mechanism of injury and ophthalmic examination is essential so that prompt treatment can be given to improve the visual prognosis of affected patients.
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http://dx.doi.org/10.3390/vision5010011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930941PMC
February 2021

Systemic corticosteroid use in UK Uveitis practice: results from the ocular inflammation steroid toxicity risk (OSTRICH) study.

Eye (Lond) 2021 Feb 2. Epub 2021 Feb 2.

Oxford Eye Hospital, Oxford University Hospitals NHS Trust, Oxford, England.

Objectives: To ascertain adherence to an international consensus target of ≤7.5 mg/day of prednisolone for maintenance systemic corticosteroid (CS) prescribing in uveitis and report the frequency of courses of high-dose systemic CS in the UK.

Methods: We conducted a national, multicentre audit of systemic CS prescribing for uveitis at 11 UK sites between November 2018 and March 2019. High-dose CS was defined as (1) maintenance >7.5 mg prednisolone for >3 consecutive months, or (2) >1 course ≥40 mg oral CS or ≥500 mg intravenous (IV) methylprednisolone in the past 12 months. Case notes of patients exceeding threshold CS doses were reviewed by an independent uveitis specialist and judged as avoidable or not, based upon a scoring matrix.

Results: Of 667 eligible patients, 285 (42.7%) were treated with oral or IV CS over the preceding 12 months; 96 (33.7%) of these exceeded the threshold for high-dose CS. Twenty-five percent of prescribing in patients on excess CS was judged avoidable; attributed to either prescribing long-term CS without evidence of consideration of alternative strategies, prescribing error or miscommunication. More patients received immunomodulatory therapy (IMT) in the group treated with CS above threshold than below threshold (p < 0.001) but there was no significant difference in doses of IMT.

Conclusion: 33% of patients had been prescribed excessive corticosteroid when compared to the reference standard. An analysis of decision-making suggests there may be opportunity to reduce excess CS prescribing in 25% of these patients.
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http://dx.doi.org/10.1038/s41433-020-01336-6DOI Listing
February 2021

Implementation of the LOOP pathway-a framework for the management of ocular tuberculosis across the United Kingdom?

Eye (Lond) 2021 Jan 14. Epub 2021 Jan 14.

Leicester Royal Infirmary, Infirmary Square, Leicester, LE1 5WW, UK.

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http://dx.doi.org/10.1038/s41433-020-01348-2DOI Listing
January 2021

Genotype-independent and enhanced in planta mediated genetic transformation of peanut [ (L.)].

3 Biotech 2018 Apr 29;8(4):202. Epub 2018 Mar 29.

1Department of Biotechnology, Bharathidasan University, Tiruchirappalli, 620024 Tamil Nadu India.

infection and regeneration of the putatively transformed plant from the explant remains arduous for some crop species like peanut. Henceforth, a competent and reproducible in planta genetic transformation protocol is established for peanut cv. CO7 by standardizing various factors such as pre-culture duration, acetosyringone concentration, duration of co-cultivation, sonication and vacuum infiltration. In the present investigation, strain EHA105 harboring the binary vector pCAMBIA1301- was used for transformation. The two-stage selection was carried out using 4 and 250 mg l BASTA to completely eliminate the chimeric and non-transformed plants. The transgene integration into plant genome was evaluated by GUS histochemical assay, polymerase chain reaction (PCR), and Southern blot hybridization. Among the various combinations and concentrations analyzed, highest transformation efficiency was obtained when the 2-day pre-cultured explants were subjected to sonication for 6 min and vacuum infiltrated for 3 min in suspension, and co-cultivated on MS medium supplemented with 150 µM acetosyringone for 3 days. The fidelity of the standardized in planta transformation method was assessed in five peanut cultivars and all the cultivars responded positively with a transformation efficiency ranging from minimum 31.3% (with cv. CO6) to maximum 38.6% (with cv. TMV7). The in planta transformation method optimized in this study could be beneficial to develop superior peanut cultivars with desirable genetic traits.
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http://dx.doi.org/10.1007/s13205-018-1231-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874222PMC
April 2018

A case of Phoma fungal keratitis in a contact lens user.

JRSM Open 2015 Mar 18;6(3):2054270415577760. Epub 2015 Mar 18.

Department of Ophthalmology, Leicester Royal Infirmary, Leicester, LE1 5WW, UK.

Fungi of the genus Phoma are common plant pathogens and saprophytes and are rarely pathogenic to animals.
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http://dx.doi.org/10.1177/2054270415577760DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372568PMC
March 2015

Macular morphology in patients with optic nerve head drusen and optic disc edema.

Ophthalmology 2014 Feb 14;121(2):552-7. Epub 2013 Nov 14.

Ophthalmology Group, University of Leicester, Robert Kilpatrick Clinical Sciences Building, Leicester Royal Infirmary, Leicester, United Kingdom. Electronic address:

Purpose: In this study we investigated macular morphology, including individual retinal layers, in patients with optic nerve head drusen (ONHD) and optic disc edema (ODE) compared with healthy participants, using high-resolution spectral domain optical coherence tomography (OCT).

Design: Prospective, cross-sectional, observational study.

Participants: A total of 67 patients with ONHD, 36 patients with ODE, and 57 healthy participants.

Methods: High-resolution spectral domain OCT (Copernicus [OPTOPOL Technology S.A., Zawiercie, Poland] 3-μm resolution, 7 × 7 × 2-mm volumetric scans) was used to image macula morphology. Average retinal nerve fiber layer (RNFL) thickness was measured using a semiautomated method with manual correction of the internal limiting membrane, RNFL, and retinal pigment epithelium (RPE). Retinal and RNFL thicknesses were measured and analyzed in 3 circular zones (Early Treatment Diabetic Retinopathy Study protocol). Individual retinal layers at the macula were quantified by analyzing tomograms using ImageJ (http://rsbweb.nih.gov/ij/; Accessed June 1, 2013).

Main Outcome Measures: Average retinal and individual retinal layer thickness in patients with ODE or ONHD, and healthy controls.

Results: Patients with ONHD had thicker retinae in the inner annulus compared with patients with ODE and controls (significant in the temporal segment compared with those with ODE [P = 0.013] and in the superior segment compared with controls [P = 0.05]). Patients with ONHD had a significantly thinner inner plexiform layer (IPL) (P = 0.02), nerve fiber layer (P = 0.05), and RPE (P = 0.0001), and thicker ganglion cell layer (P = 0.003) and outer plexiform layer (OPL) (P < 0.001) compared with controls. Patients with ODE demonstrated the thickest retina and RNFL in the outer annulus (significant in the inferior segment compared with controls, P = 0.02 for both) with significant thickening in the IPL (P = 0.004), OPL (P < 0.003), and outer segment layer (P ≤ 0.02), and severe ganglion cell loss (P = 0.004) and RPE (P = 0.0001) thinning compared with healthy volunteers.

Conclusions: Our study shows that optic nerve diseases are associated with selective changes in different retinal layers in patients with ODE and ONHD. These findings may be of diagnostic value and could be taken into consideration in assessing patients and studying the pathogenesis of these conditions.
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http://dx.doi.org/10.1016/j.ophtha.2013.09.037DOI Listing
February 2014

Sclerochoroidal calcification associated with Albright's hereditary osteodystrophy.

BMJ Case Rep 2012 Jul 19;2012. Epub 2012 Jul 19.

Department of Cardiovascular Sciences, Univeristy of Leicester, Leicester, UK.

A 47-year-old woman presented with bilateral gradual loss of vision, ocular discomfort and seeing a black shadow in her right visual field over 6 months duration. Her medical history was extensive including: developmental delay, pseudohypoparathyroidism, hypertension, spinal stenosis, epilepsy and suspected idiopathic intracranial hypertension. Ocular examination revealed choroidal elevation in both eyes, which were highly ecogenic on ecography and confirmed to be calcifications of choroids on CT scan in her both eyes. She had subnormal vision and reduced colour vision in her both eyes. Electrodiagostic studies suggested rod dysfunction. She had typical features of Albright's hereditary dystrophy and was positive for the GNAS 1 mutation. She is currently being monitored by ophthalmologlists and is also under a medical team undergoing further assessment with regard to her treatment.
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http://dx.doi.org/10.1136/bcr-03-2012-6022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4543102PMC
July 2012

Spontaneous bleb formation.

Clin Exp Ophthalmol 2011 Sep-Oct;39(7):712. Epub 2011 Apr 4.

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http://dx.doi.org/10.1111/j.1442-9071.2011.02526.xDOI Listing
June 2012