Publications by authors named "Peng Jin"

847 Publications

Enhanced nitrogen removal from low-temperature wastewater by an iterative screening of cold-tolerant denitrifying bacteria.

Bioprocess Biosyst Eng 2021 Dec 2. Epub 2021 Dec 2.

School of Environmental and Resource, Zhejiang A and F University, Hangzhou, 311300, China.

The biological process to remove nitrogen in winter effluent is often seriously compromised due to the effect of low temperatures (< 13 °C) on the metabolic activity of microorganisms. In this study, a novel heterotrophic nitrifying-aerobic denitrifying bacterium with cold tolerance was isolated by iterative domestication and named Moraxella sp. LT-01. The LT-01 maintained almost 60% of its maximal growth activity at 10 °C. Under initial concentrations of 100 mg/L, the removal efficiencies of ammonium, nitrate, nitrite by LT-01 were 70.3%, 65.4%, 61.7% respectively for 72 h incubation at 10 °C. Nitrogen balance analysis showed that about 46% of TN was released as gases and 16% of TN was assimilated for cell growth. The biomarker genes involved in nitrification and denitrification pathways were identified by gene-specific PCR and revealed that the LT-01 has nitrite reductase (NirS) but not hydroxylamine reductase (HAO), which implies the involvement of other genes in the process. The study indicates that LT-01 has the potential for use in low-temperature regions for efficient sewage treatment.
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http://dx.doi.org/10.1007/s00449-021-02668-7DOI Listing
December 2021

An oral vaccine based on chitosan/aluminum adjuvant induces both local and systemic immune responses in turbot (Scophthalmus maximus).

Vaccine 2021 Nov 26. Epub 2021 Nov 26.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai 200237, China. Electronic address:

Oral vaccination is the most convenient method for disease protection in aquaculture due to possibility of adding vaccines as part of fish diet. However, low protective efficiency is the major problem of oral vaccination owing to some reasons, especially for antigens degradation. In this study, we developed an oral inactivated vaccine based on a chitosan/aluminum adjuvant system, and investigated immune response induced by the vaccine. As a result, Th1 and Th2 cells mediated immune responses were observed after the vaccination according to the upregulation of IL-6, IL-12, IFNγ and IgM genes expression that were deemed as the driver cytokines for triggering the responses. Moreover, ratio of IgT/IgM B cells was elevated in intestine after vaccination, while IgM antibodies were also observed in the sera of vaccinated fish, suggested that both local and systemic immune responses were induced by the inactivated vaccine. Totally, this oral vaccine can be used for V. anguillarum protection and this chitosan/aluminum system is an efficient candidate adjuvant for developing more vaccines in the future.
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http://dx.doi.org/10.1016/j.vaccine.2021.10.063DOI Listing
November 2021

The Deubiquitinase USP28 Stabilizes Expression of RecQ Family Helicases and Maintains the Viability of Triple Negative Breast Cancer Cells.

J Biol Chem 2021 Nov 22:101443. Epub 2021 Nov 22.

Zhejiang Provincial Key Laboratory of Pancreatic Disease, The First Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang 310003, China. Electronic address:

Triple-negative breast cancer lacks significant expression of the estrogen receptor, the progesterone receptor, and of human epidermal growth factor receptor. It is the most aggressive and malignant of all breast cancers, and for which there are currently no effective targeted therapies. We have shown previously that the RecQ helicase family member RECQL5 is essential for the proliferation and survival of TNBC cells; however, the mechanism of its involvement in cell viability has not been shown. Here we report that the expression of RecQ family helicases, including RECQL5, are regulated by the deubiquitinase USP28. We found using genetic depletion or a small molecule inhibitor that like RECQL5, USP28 is also essential for TNBC cells to proliferate in vitro and in vivo. Compromising the function of USP28 by shRNA knockdown or the inhibitor caused TNBC cells to arrest in S/G2 phases, concurrent with DNA damage checkpoint activation. We further showed that the small molecule inhibitor of USP28 displayed anti-tumor activity against xenografts derived from TNBC cells. Our results suggest that USP28 could be a potential therapeutic target for triple negative breast cancer.
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http://dx.doi.org/10.1016/j.jbc.2021.101443DOI Listing
November 2021

Modified Systemic Inflammation Score Is an Independent Predictor of Long-Term Outcome in Patients Undergoing Surgery for Adenocarcinoma of the Esophagogastric Junction.

Front Surg 2021 8;8:622821. Epub 2021 Nov 8.

Department of Pancreatic and Gastric Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.

The modified systemic inflammation score (mSIS), which is calculated by a composite score of the lymphocyte-to-monocyte ratio and the albumin content in serum, is identified as the new score to predict the prognosis for various cancers. However, its significance for patients with adenocarcinoma of esophagogastric junction (AEJ), who receive surgery, remains unclear. This study retrospectively analyzed 317 patients with AEJ receiving surgery between September 2010 and December 2016. The associations between the mSIS and the clinicopathological features, overall survival (OS), as well as relapse-free survival (RFS), were assessed. In addition, the time-dependent receiver operating characteristic (t-ROC) curve analysis was performed for comparing the value of those scoring systems in predicting patient prognosis. Of the 317 cases, 119 were rated as mSIS 0, 123 as mSIS 1, and 75 as mSIS 2. Besides, mSIS was significantly related to age and tumor size. On multivariate analysis, mSIS was identified as a predictor to independently predict OS ( < 0.001) along with RFS ( < 0.001), and a significantly strong correlation was observed at the advanced pTNM stages based on the mSIS system. In the subgroup analysis of adjuvant chemotherapy and surgery alone, mSIS was still the predictor for independently predicting patient OS ( < 0.001) together with RFS ( < 0.001) for the two groups. T-ROC analysis showed that mSIS was more accurate than controlling nutritional status score in predicting OS and RFS. The mSIS can serve as an easy, useful scoring system to independently predict the preoperative survival for AEJ cases undergoing surgery.
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http://dx.doi.org/10.3389/fsurg.2021.622821DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606684PMC
November 2021

Channeled imaging spectropolarimeter reconstruction by neural networks.

Opt Express 2021 Oct;29(22):35556-35569

Snapshot channeled imaging spectropolarimetry (SCISP), which can achieve spectral and polarization imaging without scanning (a single exposure), is a promising optical technique. As Fourier transform is used to reconstruct information, SCISP has its inherent limitations such as channel crosstalk, resolution and accuracy drop, the complex phase calibration, et al. To overcome these drawbacks, a nonlinear technique based on neural networks (NNs) is introduced to replace the role of Fourier reconstruction. Herein, abundant spectral and polarization datasets were built through specially designed generators. The established NNs can effectively learn the forward conversion procedure through minimizing a loss function, subsequently enabling a stable output containing spectral, polarization, and spatial information. The utility and reliability of the proposed technique is confirmed by experiments, which are proved to maintain high spectral and polarization accuracy.
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http://dx.doi.org/10.1364/OE.441850DOI Listing
October 2021

Analysis of the characteristics of paulownia lignocellulose and hydrogen production potential via photo fermentation.

Bioresour Technol 2021 Nov 18;344(Pt B):126361. Epub 2021 Nov 18.

Institute of Agricultural Engineering, Huanghe S & T University, Zhengzhou 450006, China.

Paulownia biomass is rich in carbohydrates, making which a potential feedstock for biohydrogen production. In the study, different parts and varieties of Paulownia were chose as substrates to evaluate hydrogen production potential of paulownia lignocellulose via biohydrogen production by photo fermentation (BHPPF) and energy conversion efficiency (ECE). Results showed the highest cumulative hydrogen yield (CHY) of 67.11 mL/g total solids (TS) and ECE of 4.74% were obtained from leaves of Paulownia, which were 121.06% and 115.45% higher than those of the branches. Moreover, Paulownia jianshiensis leaves were found to be the best variety for BHPPF, with the maximum CHY of 98.83 mL/g TS and ECE of 7.18%. Using Paulownia waste as the substrate to produce hydrogen helps broaden the range of raw materials for BHPPF and improve the economic utilization of forestry waste.
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http://dx.doi.org/10.1016/j.biortech.2021.126361DOI Listing
November 2021

Additive impacts of ocean acidification and ambient ultraviolet radiation threaten calcifying marine primary producers.

Sci Total Environ 2021 Nov 18:151782. Epub 2021 Nov 18.

School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, China. Electronic address:

Ocean acidification (OA) represents a threat to marine organisms and ecosystems. However, OA rarely exists in isolation but occurs concomitantly with other stressors such as ultraviolet radiation (UVR), whose effects have been neglected in oceanographical observations. Here, we perform a quantitative meta-analysis based on 373 published experimental assessments from 26 studies to examine the combined effects of OA and UVR on marine primary producers. The results reveal predominantly additive stressor interactions (69-84% depending on the UV waveband), with synergistic and antagonistic interactions being rare but significantly different between micro- and macro-algae. In microalgae, variations in interaction type frequencies are related to cell volume, with antagonistic interactions accounting for a higher proportion in larger sized species. Despite additive interactions being most frequent, the small proportion of antagonistic interactions appears to have a stronger power, leading to neutral effects of OA in combination with UVR. High levels of UVR at near in situ conditions in combination with OA showed additive inhibition of calcification, but not when UVR was low. The results also reveal that the magnitude of responses is strongly dependent on experimental duration, with the negative effects of OA on calcification and pigmentation being buffered and amplified by increasing durations, respectively. Tropical primary producers were more vulnerable to OA or UVR alone compared to conspecifics from other climatic regions. Our analysis highlights that further multi-stressor long-term adaptation experiments with marine organisms of different cell volumes (especially microalgae) from different climatic regions are needed to fully disclose future impacts of OA and UVR.
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http://dx.doi.org/10.1016/j.scitotenv.2021.151782DOI Listing
November 2021

A Comparison of Incomplete Resection Rate of Large and Small Colorectal Polyps by Cold Snare Polypectomy.

Clin Gastroenterol Hepatol 2021 Nov 16. Epub 2021 Nov 16.

Department of Gastroenterology, The Seventh Medical Center of Chinese PLA General Hospital, Beijing, China;; The Second School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong, China;; Department of Gastroenterology, The Third Affiliated Hospital of Jinzhou Medical University, Panjin Liaohe Oilfield Gem Flower Hospital, Panjin, Liaoning, China;. Electronic address:

Background & Aims: There are limited data regarding the safety and efficacy of cold snare polypectomy (CSP) for large colorectal polyps. We evaluated factors affecting the clinical outcomes of CSP for polyps between 5 and 15 mm in size.

Methods: This was a prospective single-center observational study involving 1000 patients undergoing colonoscopy. Polyps (5-15 mm) were removed using CSP, and biopsies were taken from the resection margin. The primary outcome was the incomplete resection rate (IRR), and was determined by the presence of residual neoplasia on biopsy. Correlations between IRR and polyp size, morphology, histology, resection time were assessed by generalized estimating equation (GEE) model.

Results: A total of 440 neoplastic polyps were removed from 261 patients. The overall IRR was 2.27%, 1.98% for small (5-9 mm) vs 3.45% for large (10-15 mm) polyps, P=0.411). In univariate analysis, the IRR was more likely to be related to SSL (OR=6.93, 95% CI 1.88-25.45, P=0.004), piecemeal resection (OR=11.83, 95% CI 1.20-116.49, P=0.034) and prolonged resection time >60s (OR=7.56, 95% CI 1.75-32.69, P=0.007). In multivariable regression analysis, SSL (OR=6.45, 95% CI 1.48-28.03, P=0.013) and resection time (OR=7.39, 95% CI 1.48-36.96, P=0.015, respectively) were independent risk factors for IRR. Immediate bleeding was more frequent with resection of large polyps (6.90% vs 1.42%, P=0.003). No recurrence was seen on follow-up colonoscopy in 37 cases with large polyps.

Conclusions: CSP is safe and effective for removal of colorectal polyps up to 15 mm in size, with a low IRR.
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http://dx.doi.org/10.1016/j.cgh.2021.11.010DOI Listing
November 2021

Dexmedetomidine alleviates cognitive impairment by reducing blood-brain barrier interruption and neuroinflammation via regulating Th1/Th2/Th17 polarization in an experimental sepsis model of mice.

Int Immunopharmacol 2021 Nov 13:108332. Epub 2021 Nov 13.

Department of Critical Care Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China. Electronic address:

Clinical studies have shown that dexmedetomidine (DEX) reduces mortality and inflammation in patients with sepsis, and ameliorates cognitive decline in both postoperative and critical care patients. This study aims to explain the neuroprotective effects provided by DEX in mice with cecal ligation and puncture (CLP)-induced polymicrobial sepsis. Mice were treated with DEX intraperitoneally three times every two hours after CLP. The survival rate, body weight, and clinical scores were recorded each day. Morris water maze (MWM) and fear conditioning tests were used to evaluate cognitive function. Blood brain barrier (BBB) permeability, hippocampal inflammation, hippocampal neural apoptosis, and T helper (Th) cell subgroups were assessed. Furthermore, Atipamezole was used to verify that the potential neuroprotective effects in the sepsis-associated encephalopathy (SAE) were mediated by DEX. Compared with the Sham group, CLP mice showed significant cognitive impairment, BBB interruption, excessive neuroinflammation, and neuronal apoptosis. These detrimental effects of CLP were attenuated by DEX. Furthermore, we found that DEX corrects peripheral Th1/Th2/Th17 shift and reduces proinflammatory cytokines in the hippocampus. Additionally, atipamezole prevented DEX's protective effect. Taken together, DEX alleviates cognitive impairments by reducing blood-brain barrier interruption and neuroinflammation by regulating Th1/Th2/Th17 polarization.
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http://dx.doi.org/10.1016/j.intimp.2021.108332DOI Listing
November 2021

Comparison of the value of ultrasound and enhanced magnetic resonance imaging in judging cervical lymph node metastasis in patients with oral cancer.

Bull Cancer 2021 Nov 12. Epub 2021 Nov 12.

Sun Yat-sen University Cancer Center, Department of Head and Neck Surgery, 51, Dongfeng Dong Road, Guangzhou, 510060, China; State key laboratory of Oncology in South China, Guangzhou, China; Collaborative Innovation Center for Cancer Medicine, Guangzhou, China. Electronic address:

Background: Oral cancer is the twelfth largest malignant tumor in the world. Oral cancer is prone to lymph node metastasis in the early stages of the disease, and lymph node metastasis will directly affect the treatment and survival rate. Therefore, whether the lymph node metastasis can be accurately evaluated is of great significance to the treatment and prognosis of patients.

Objective: The diagnostic efficacy of three methods of preoperative ultrasound, enhanced magnetic resonance (enhanced MR), and ultrasound combined with enhanced MR on cervical lymph node metastasis of oral cancer were compared.

Materials And Methods: A retrospective analysis of 399 cases of oral cancer completed by head and neck surgery at Cancer Center of Sun Yat-sen University, China. In all cases, the maxillofacial and neck enhanced MR and cervical lymph node ultrasound examinations were performed before surgery, and imaging diagnosis was made for cervical lymph node metastasis and compared with pathology. All judgment results were statistically processed using the chi-square test.

Results: Compared with the above three methods, the diagnostic efficiency of ultrasound alone is better, and the diagnostic efficiency of enhanced MR combined ultrasound is not significantly better than that of ultrasound alone. There are differences in the diagnostic efficacy of the three inspection methods in different clinical sub-periods, all of which are better for the diagnosis of stage I. For patients with stage I, II, and III, there were no differences between the three examination methods. However, for stage IV patients,the diagnostic efficiency of ultrasound alone is better. For patients with stage N0, there was no difference in diagnostic efficacy between the three methods. For the assessment of lymph nodes in the I-III region, there are differences between the three methods of examination: the diagnostic efficiency of ultrasound alone is better, and enhanced MR combined ultrasound is not significantly better than ultrasound alone. For the evaluation of lymph nodes in the IV-VII region, there was no difference in diagnostic efficacy between the three methods.

Conclusion: According to the clinical characteristics and prognosis of patients with oral cancer, although enhanced MR can better evaluate the tumor, ultrasound may be considered as the preferred imaging method for cervical lymph node metastasis.

Significance: Accurate assessment and proper and timely treatment of lymph node metastasis can help improve the survival rate of patients.
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http://dx.doi.org/10.1016/j.bulcan.2021.09.010DOI Listing
November 2021

Retrospective analysis of surgically treated pT4b gastric cancer with pancreatic head invasion.

World J Clin Cases 2021 Oct;9(29):8718-8728

Department of Pancreatic and Gastric Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.

Background: For advanced gastric cancer patients with pancreatic head invasion, some studies have suggested that extended multiorgan resections (EMR) improves survival. However, other reports have shown high rates of morbidity and mortality after EMR. EMR for T4b gastric cancer remains controversial.

Aim: To evaluate the surgical approach for pT4b gastric cancer with pancreatic head invasion.

Methods: A total of 144 consecutive patients with gastric cancer with pancreatic head invasion were surgically treated between 2006 and 2016 at the China National Cancer Center. Gastric cancer was confirmed in 76 patients by postoperative pathology and retrospectively analyzed. The patients were divided into the gastrectomy plus pancreaticoduodenectomy group (GP group) and gastrectomy alone group (GA group) by comparing the clinicopathological features, surgical outcomes, and prognostic factors of these patients.

Results: There were 24 patients (16.8%) in the GP group who had significantly larger lesions ( < 0.001), a higher incidence of advanced N stage ( = 0.030), and less neoadjuvant chemotherapy ( < 0.001) than the GA group had. Postoperative morbidity (33.3% 15.3%, = 0.128) and mortality (4.2% 4.8%, = 1.000) were not significantly different in the GP and GA groups. The overall 3-year survival rate of the patients in the GP group was significantly longer than that in the GA group (47.6%, median 30.3 mo 20.4%, median 22.8 mo, = 0.010). Multivariate analysis identified neoadjuvant chemotherapy [hazard ratio (HR) 0.290, 95% confidence interval (CI): 0.103-0.821, = 0.020], linitis plastic (HR 2.614, 95% CI: 1.024-6.675, = 0.033), surgical margin (HR 0.274, 95% CI: 0.102-0.738, = 0.010), N stage (HR 3.489, 95% CI: 1.334-9.120, = 0.011), and postoperative chemoradiotherapy (HR 0.369, 95% CI: 0.163-0.836, = 0.017) as independent predictors of survival in patients with pT4b gastric cancer and pancreatic head invasion.

Conclusion: Curative resection of the invaded pancreas should be performed to improve survival in selected patients. Invasion of the pancreatic head is not a contraindication for surgery.
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http://dx.doi.org/10.12998/wjcc.v9.i29.8718DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8546839PMC
October 2021

Cerebral perfusion and neurological examination characterise neonatal opioid withdrawal syndrome: a prospective cohort study.

Arch Dis Child Fetal Neonatal Ed 2021 Nov 1. Epub 2021 Nov 1.

Pediatrics, Emory University, Atlanta, Georgia, USA.

Objective: To test the hypothesis that cerebral blood flow (CBF) assessed with arterial spin labelling (ASL) MRI is increased and standardised neurological examination is altered in infants with neonatal opioid withdrawal syndrome (NOWS) compared with those without.

Design: Prospective cohort study.

Setting: Level IV neonatal intensive care unit and outpatient primary care centre.

Participants: Infants with NOWS receiving pharmacological treatment and unexposed controls matched for gestational age at birth and post-menstrual age at MRI.

Main Outcomes: CBF assessed by ASL on non-sedated 3-Tesla MRI and standardised Hammersmith Neonatal Neurological Examination (HNNE) within 14 days of birth.

Results: Thirty infants with NOWS and 31 control infants were enrolled and included in the final analysis. Global CBF across the brain was higher in the NOWS group compared with controls (14.2 mL/100 g/min±5.5 vs 10.7 mL/100 g/min±4.3, mean±SD, Cohen's d=0.72). HNNE total optimality score was lower in the NOWS group compared with controls (25.9±3.6 vs 28.4±2.4, mean±SD, Cohen's d=0.81). A penalised logistic regression model including both CBF and HNNE items discriminated best between the two groups.

Conclusions: Increased cerebral perfusion and neurological examination abnormalities characterise infants with NOWS compared with those without intrauterine drug exposure and suggest prenatal substance exposure affects fetal brain development. Identifying neurological and neuroimaging characteristics of infants with NOWS can contribute to understanding mechanisms underlying later outcomes and to designing potential new treatments.
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http://dx.doi.org/10.1136/archdischild-2021-322192DOI Listing
November 2021

Lipid Remodeling Reveals the Adaptations of a Marine Diatom to Ocean Acidification.

Front Microbiol 2021 14;12:748445. Epub 2021 Oct 14.

School of Environmental Science and Engineering, Guangzhou University, Guangzhou, China.

Ocean acidification is recognized as a major anthropogenic perturbation of the modern ocean. While extensive studies have been carried out to explore the short-term physiological responses of phytoplankton to ocean acidification, little is known about their lipidomic responses after a long-term ocean acidification adaptation. Here we perform the lipidomic analysis of a marine diatom following long-term (∼400 days) selection to ocean acidification conditions. We identified a total of 476 lipid metabolites in long-term high CO (i.e., ocean acidification condition) and low CO (i.e., ambient condition) selected cells. Our results further show that long-term high CO selection triggered substantial changes in lipid metabolites by down- and up-regulating 33 and 42 lipid metabolites. While monogalactosyldiacylglycerol (MGDG) was significantly down-regulated in the long-term high CO selected conditions, the majority (∼80%) of phosphatidylglycerol (PG) was up-regulated. The tightly coupled regulations (positively or negatively correlated) of significantly regulated lipid metabolites suggest that the lipid remodeling is an organismal adaptation strategy of marine diatoms to ongoing ocean acidification. Since the composition and content of lipids are crucial for marine food quality, and these changes can be transferred to high trophic levels, our results highlight the importance of determining the long-term adaptation of lipids in marine producers in predicting the ecological consequences of climate change.
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http://dx.doi.org/10.3389/fmicb.2021.748445DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551959PMC
October 2021

The Phenotypes and Mechanisms of NOTCH2NLC-Related GGC Repeat Expansion Disorders: a Comprehensive Review.

Mol Neurobiol 2021 Oct 31. Epub 2021 Oct 31.

Department of Neurology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.

The human-specific gene NOTCH2NLC is primarily expressed in radial glial cells and plays an important role in neuronal differentiation and cortical neurogenesis. Increasing studies were conducted to verify the relationship between NOTCH2NLC gene and many neurological diseases, such as neuronal intranuclear inclusion disease, essential tremor, multiple system atrophy, Parkinson's disease, Alzheimer's disease, and even oculopharyngodistal myopathy. Thus, we support the concept, NOTCH2NLC-related GGC repeat expansion disorders (NRED), to summarize all diseases with the GGC repeat expansion in the 5'UTR of NOTCH2NLC gene, regardless of their various clinical phenotypes. Here, we discuss the reported cases to analyze the clinical features of NOTCH2NLC-related GGC repeat expansion disorders, including dementia, parkinsonism, peripheral neuropathy and myopathy, leukoencephalopathy, and essential tremor. In addition, we outline radiological and pathological manifestations of NOTCH2NLC-related GGC repeat expansion disorders, and then present possible mechanisms, such as toxic polyG protein, toxic repeat RNA, the GGC repeat size, and the size and types of trinucleotide interruption. Therefore, this review provides a systematic description of NOTCH2NLC-related GGC repeat expansion disorders and emphasizes the significance for understanding this type of repeat expansion disease.
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http://dx.doi.org/10.1007/s12035-021-02616-2DOI Listing
October 2021

Application of Bacillus mucilaginosus in the carbonation of steel slag.

Appl Microbiol Biotechnol 2021 Dec 30;105(23):8663-8674. Epub 2021 Oct 30.

College of Materials Science and Engineering, Southeast University, Nanjing, 211189, People's Republic of China.

The stacking of steel slag has detrimental effects mainly for the waste of resources and the pollution of environment. In this study, a novel method based on microbially induced calcium precipitation (MICP) was proposed by utilizing a type of microorganism named Bacillus mucilaginosus, which could secrete carbonic anhydrase (CA) through the metabolism process, accelerating the hydration of carbon dioxide (CO) and thus facilitating the formation of carbonate ions (CO). First, comparing the biologically deposited calcium carbonate with the chemically deposited one, it was found that the crystallinity and crystal size of the biological deposition was lower, leading to its cementitious properties. Under the condition of 1 wt. (weight) % dosage, the carbonation degree increased from 66.34 to 86.25% and the compressive strength improved greatly from 7.4 to 11.2 MPa as well. The weight gain rate of biologically carbonated specimens was also twice as much as the directly carbonated ones. This work strongly demonstrated that biological carbonation technology could not only improve the CO sequestration potential of steel slag but also enhance the mechanical properties and durability of steel slag products. KEY POINTS: • Bacillus mucilaginosus could resuscitate and proliferate in the steel slag environment. • B. mucilaginosus secreted carbon anhydrase, which could accelerate the hydration of CO and facilitate the precipitation of calcium carbonate. • Biologically carbonated steel slag had greater mechanical performance than directly carbonated one.
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http://dx.doi.org/10.1007/s00253-021-11641-zDOI Listing
December 2021

Prognostic, clinicopathological, and immune correlation of NLRP3 promoter methylation in kidney renal clear cell carcinoma.

Clin Transl Med 2021 10;11(10):e528

State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.

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http://dx.doi.org/10.1002/ctm2.528DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8530444PMC
October 2021

Flexible Doppler ultrasound device for the monitoring of blood flow velocity.

Sci Adv 2021 Oct 27;7(44):eabi9283. Epub 2021 Oct 27.

AML, Department of Engineering Mechanics, Tsinghua University, Beijing 100084, China.

[Figure: see text].
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http://dx.doi.org/10.1126/sciadv.abi9283DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8550238PMC
October 2021

Pan-Cancer Analysis of Glycolytic and Ketone Bodies Metabolic Genes: Implications for Response to Ketogenic Dietary Therapy.

Front Oncol 2021 7;11:689068. Epub 2021 Oct 7.

Department of Hepatobiliary Surgery, The Affiliated Drum Tower Hospital, Medical School of Nanjing University, Nanjing, China.

Background: The Warburg effect, also termed "aerobic glycolysis", is one of the most remarkable and ubiquitous metabolic characteristics exhibited by cancer cells, representing a potential vulnerability that might be targeted for tumor therapy. Ketogenic diets (KDs), composed of high-fat, moderate-protein and low carbohydrates, are aimed at targeting the Warburg effect for cancer treatment, which have recently gained considerable attention. However, the efficiency of KDs was inconsistent, and the genotypic contribution is still largely unknown.

Methods: The bulk RNA-seq data from The Cancer Genome Atlas (TCGA), single cell RNA sequencing (scRNA-seq), and microarray data from Gene Expression Omnibus (GEO) and Cancer Cell Line Encyclopedia (CCLE) were collected. A joint analysis of glycolysis and ketone bodies metabolism (KBM) pathway was performed across over 10,000 tumor samples and nearly 1,000 cancer cell lines. A series of bioinformatic approaches were combined to identify a metabolic subtype that may predict the response to ketogenic dietary therapy (KDT). Mouse xenografts were established to validate the predictive utility of our subtypes in response to KDT.

Results: We first provided a system-level view of the expression pattern and prognosis of the signature genes from glycolysis and KBM pathway across 33 cancer types. Analysis by joint stratification of glycolysis and KBM revealed four metabolic subtypes, which correlated extensively but diversely with clinical outcomes across cancers. The glycolytic subtypes may be driven by TP53 mutations, whereas the KB-metabolic subtypes may be mediated by CTNNB1 (β-catenin) mutations. The glycolytic subtypes may have a better response to KDs compared to the other three subtypes. We preliminarily confirmed the idea by literature review and further performed a proof-of-concept experiment to validate the predictive value of the metabolic subtype in liver cancer xenografts.

Conclusions: Our findings identified a metabolic subtype based on glycolysis and KBM that may serve as a promising biomarker to predict the clinical outcomes and therapeutic responses to KDT.
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http://dx.doi.org/10.3389/fonc.2021.689068DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8529115PMC
October 2021

TNF Patterns and Tumor Microenvironment Characterization in Head and Neck Squamous Cell Carcinoma.

Front Immunol 2021 6;12:754818. Epub 2021 Oct 6.

Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Guangzhou, China.

Background: HNSCC is a heterogeneous disease, which arises from distinct anatomic subsites, associates with various risk factors and possesses diverse molecular pathological features. Generally, HNSCC is considered as an immunosuppressive disease, characterized by abnormal tumor immune microenvironment. The TNF family plays a crucial role in the survival, proliferation, differentiation, and effector functions in both immune and non-immune cells. However, the expression patterns of TNF in HNSCC remains to be systematically analyzed.

Methods: We downloaded transcriptional profile data of HNSCC from TCGA and GEO datasets. Unsupervised clustering methods were used to identify different TNF patterns and classify patients for further analysis. PCA was conducted to construct a TNF relevant score, which we called risk score.

Results: In this study, we systematically evaluated the patterns of TNF family and tumor immune microenvironment characteristics of HNSCC patients by clustering the expression of 46 members of TNF family. We identified two subtypes with distinct clinical and immune characteristics in HNSCC and constructed a risk scoring system based on the expression profile of TNF family genes.

Conclusion: Risk score serves as a reliable predictor of overall survival, clinical characteristics, and immune cell infiltration, which has the potential to be applied as a valuable biomarker for HNSCC immunotherapy.
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http://dx.doi.org/10.3389/fimmu.2021.754818DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8526904PMC
October 2021

Effects of Low Temperature, Nitrogen Starvation and Their Combination on the Photosynthesis and Metabolites of E542: A Comparison Study.

Plants (Basel) 2021 Oct 4;10(10). Epub 2021 Oct 4.

School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, China.

Both low temperature and nitrogen starvation caused chlorosis of cyanobacteria. Here, in this study, for the first time, we compared the effects of low temperature, nitrogen starvation, and their combination on the photosynthesis and metabolites of a thermophilic cyanobacterium strain, E542. Under various culture conditions, the growth rates, pigment contents, and chlorophyll fluorescence were monitored, and the composition of alkanes, lipidomes, and carbohydrates were determined. It was found that low temperature (35 °C) significantly suppressed the growth of E542. Nitrogen starvation at 45 °C and 55 °C did not affect the growth; however, combined treatment of low temperature and nitrogen starvation led to the lowest growth rate and biomass productivity. Both low temperature and nitrogen starvation caused significantly declined contents of pigments, but they resulted in a different effect on the OJIP curves, and their combination led to the lowest pigment contents. The composition of fatty acids and alkanes was altered upon low-temperature cultivation, while nitrogen starvation caused reduced contents of all lipids. The low temperature did not affect carbohydrate contents, while nitrogen starvation greatly enhanced carbohydrate content, and their combination did not enhance carbohydrate content, but led to reduced productivity. These results revealed the influence of low temperature, nitrogen starvation, and their combined treatment for the accumulation of phycobiliproteins, lipids, and carbohydrates of a thermophilic cyanobacterium strain, E542.
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http://dx.doi.org/10.3390/plants10102101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537721PMC
October 2021

The combination of body composition conditions and systemic inflammatory markers has prognostic value for patients with gastric cancer treated with adjuvant chemoradiotherapy.

Nutrition 2022 Jan 17;93:111464. Epub 2021 Sep 17.

Department of Gastrointestinal Surgery/Department of Clinical Nutrition, Beijing Shijitan Hospital, Capital Medical University, Beijing, China. Electronic address:

Objective: The aim of this study was to explore the prognostic value of the association between systemic inflammation response markers (red blood cell distribution width, neutrophil platelet score, prognostic nutritional index, neutrophil-to-lymphocyte ratio, neutrophil-to-platelet ratio, lymphocyte-to-monocyte ratio, and systemic immune-inflammation index) and poorer body composition conditions (sarcopenia, myosteatosis, and sarcopenic obesity) among patients with gastric cancer who underwent adjuvant chemoradiotherapy after radical gastrectomy.

Methods: A computed tomography scan was performed within 2 wk of prechemoradiotherapy to identify sarcopenia, myosteatosis and sarcopenic obesity. Tumor and systemic inflammatory response information was recorded. Logistic analysis was used to explore the potential risk factors associated with body composition. Univariate and multivariate Cox analyses were performed for survival analysis. A nomogram was constructed to serve as a prognostic prediction tool for the 3- and 5-y overall survival rates.

Results: The study included 223 patients (74 women and 149 men) with gastric cancer treated with adjuvant chemoradiotherapy after radical gastrectomy. The incidences of sarcopenia, myosteatosis, and sarcopenic obesity were 30%, 39%, and 16%, respectively. Logistic analysis demonstrated that a low prognostic nutritional index is a risk factor for sarcopenia, myosteatosis, and sarcopenic obesity. Based on survival analysis, stage (hazard ratio [HR], 0.44; 95% confidence interval [CI], 0.23-0.84; P = 0.01), the neutrophil platelet score (HR, 0.50; 95% CI, 0.31-0.82; P = 0.01), the prognostic nutritional index (HR, 0.40; 95% CI, 0.24-0.68; P = 0.00) and sarcopenic obesity (HR, 0.54; 95% CI, 0.31-0.93; P = 0.03) remained independent prognostic factors for overall survival. Accuracy was improved when systemic inflammation markers were incorporated into the nomogram compared with when they were excluded, and the predicted C indexes of the nomogram with and without systemic inflammatory markers were 0.71 (95% CI, 0.67-0.73) and 0.63 (95% CI, 0.57-0.68), respectively.

Conclusion: The systemic inflammatory response associated with progressive nutritional conditions and body composition conditions with systemic inflammation markers incorporated presented better prognostic value.
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http://dx.doi.org/10.1016/j.nut.2021.111464DOI Listing
January 2022

Primary Second Molar Treatment as a Predictor of Repeat General Anesthesia.

Pediatr Dent 2021 Sep;43(5):380-386

Kumar, clinical professor, The Ohio State University College of Dentistry, and attending pediatric dentists, Nationwide Children's Hospital, Columbus, Ohio, USA.

The purpose of this study was to investigate an association between treatments on the primary second molars (PSMs) under general anesthesia (GA) and odds of repeat GA. This was a retrospective study of children who received dental treatment under GA between the ages of 24 to 48 months. Descriptive statistics and logistic regression models (P<0.05) were used to test the association between the treatment of PSMs at the first dental GA visit (GA1) and the odds of receiving GA a second time (GA2) within the next 55 months post-GA1. A total of 819 children (53 percent male) with a mean (±SD) age of 36 (±seven SD) months and 3,276 PSMs were included. Only three percent of children with all PSMs covered at GA1 received GA2. The odds of GA2 significantly increased for children with any uncovered PSMs. Among children with four uncovered PSMs, 19 percent (odds ratio [OR] equals 13; 95 percent confidence interval [95% CI] equals 5.8 to 33.5; P<0.001) and among those with unerupted PSMs at GA1, 51 percent received GA2 (OR equals 62.9; 95% CI equals 23.5 to 189.2; P<0.001). In the group that received GA2, 79.1 percent of uncovered PSMs at GA1 eventually received a stainless steel crown at GA2. Restorative treatments other than stainless steel crowns were associated with higher odds of repeat general anesthesia. These findings support the preferential use of full-coverage restorations for the treatment of carious primary molars in young children undergoing GA to minimize the risk of the need for repeat GA.
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September 2021

Extraordinary transmission in an add-drop filter configuration driven by nonconservative coupling.

Opt Lett 2021 Oct;46(20):5284-5287

We proposed a nonconservative coupling scheme based on the add-drop filter configuration, in which a high factor passive microtoroid resonator is indirectly driven by an active unit, thus providing an additional coupling which might be comparable to a mode decay rate. Extraordinary scattering points are predicted when one of the supermodes becomes lossless. Specifically, when the inherent coupling strength is set at half of the mode's total decay rate, controllable transmission peaks can be realized by tuning the nonconservative coupling strength and phase delay. Our theoretic research might find potential application in tunable light steering based on non-Hermitian resonator systems.
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http://dx.doi.org/10.1364/OL.440822DOI Listing
October 2021

Activation of GPR39 with TC-G 1008 attenuates neuroinflammation via SIRT1/PGC-1α/Nrf2 pathway post-neonatal hypoxic-ischemic injury in rats.

J Neuroinflammation 2021 Oct 13;18(1):226. Epub 2021 Oct 13.

Department of Physiology and Pharmacology, School of Medicine, Loma Linda University, Loma Linda, CA, 92350, USA.

Background: Hypoxic-ischemic encephalopathy (HIE) is a severe anoxic brain injury that leads to premature mortality or long-term disabilities in infants. Neuroinflammation is a vital contributor to the pathogenic cascade post-HIE and a mediator to secondary neuronal death. As a plasma membrane G-protein-coupled receptor, GPR39, exhibits anti-inflammatory activity in several diseases. This study aimed to explore the neuroprotective function of GPR39 through inhibition of inflammation post-hypoxic-ischemic (HI) injury and to elaborate the contribution of sirtuin 1(SIRT1)/peroxisome proliferator-activated receptor-γ coactivator 1α (PGC-1α)/nuclear factor, erythroid 2 like 2(Nrf2) in G-protein-coupled receptor 39 (GPR39)-mediated protection.

Methods: A total of 206 10-day-old Sprague Dawley rat pups were subjected to HIE or sham surgery. TC-G 1008 was administered intranasally at 1 h, 25 h, 49 h, and 73 h post-HIE induction. SIRT1 inhibitor EX527, GPR39 CRISPR, and PGC-1α CRISPR were administered to elucidate the underlying mechanisms. Brain infarct area, short-term and long-term neurobehavioral tests, Nissl staining, western blot, and immunofluorescence staining were performed post-HIE.

Results: The expression of GPR39 and pathway-related proteins, SIRT1, PGC-1α and Nrf2 were increased in a time-dependent manner, peaking at 24 h or 48-h post-HIE. Intranasal administration of TC-G 1008 reduced the percent infarcted area and improved short-term and long-term neurological deficits. Moreover, TC-G 1008 treatment significantly increased the expression of SIRT1, PGC-1α and Nrf2, but downregulated the expressions of IL-6, IL-1β, and TNF-α. GPR39 CRISPR EX527 and PGC-1α CRISPR abolished GPR39's neuroprotective effects post-HIE.

Conclusions: TC-G 1008 attenuated neuroinflammation in part via the SIRT1/PGC-1α/Nrf2 pathway in a neonatal rat model of HIE. TC-G 1008 may be a novel therapeutic target for treatment post-neonatal HIE injury.
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http://dx.doi.org/10.1186/s12974-021-02289-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8513331PMC
October 2021

Disease category-specific annotation of variants using an ensemble learning framework.

Brief Bioinform 2021 Oct 13. Epub 2021 Oct 13.

NCMIS, CEMS, RCSDS, Academy of Mathematics and Systems Science, Chinese Academy of Sciences, Beijing 100190, China.

Understanding the impact of non-coding sequence variants on complex diseases is an essential problem. We present a novel ensemble learning framework-CASAVA, to predict genomic loci in terms of disease category-specific risk. Using disease-associated variants identified by GWAS as training data, and diverse sequencing-based genomics and epigenomics profiles as features, CASAVA provides risk prediction of 24 major categories of diseases throughout the human genome. Our studies showed that CASAVA scores at a genomic locus provide a reasonable prediction of the disease-specific and disease category-specific risk prediction for non-coding variants located within the locus. Taking MHC2TA and immune system diseases as an example, we demonstrate the potential of CASAVA in revealing variant-disease associations. A website (http://zhanglabtools.org/CASAVA) has been built to facilitate easily access to CASAVA scores.
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http://dx.doi.org/10.1093/bib/bbab438DOI Listing
October 2021

Ontogenetic moulting behavior of the Cambrian oryctocephalid trilobite .

PeerJ 2021 23;9:e12217. Epub 2021 Sep 23.

College of Resources and Environmental Engineering, Key Laboratory of Karst Georesources and Environment, Ministry of Education, Guizhou University, Guiyang, Guizhou Province, China.

Moulting behaviors in trilobites are a crucial strategy during development. Previous studies have demonstrated inter-and intraspecific variability of moulting behavior in trilobites. Currently, ecdysial motifs for trilobites are considered not stable even within species and fewer detailed studies dealt with moulting behaviors in a single species of trilobite during development. Here a large sample of meraspid to holaspid exuviae of (131 specimens) from the Cambrian Balang Formation of South China has allowed description of the reasonably complete ontogenic moulting sequence. Both ontogenetic stage and body size reveal gradual transition of configuration from Somersault configuration to Henningsmoen's configuration during development. Somersault configuration is exclusive till meraspid degree five and exists in subsequent growth stages. This suggests that opening of the facial and rostral sutures allowing the emergence forward of the post-ecdysial trilobite was prevalent in early growth stages. In later development, Henningsmoen's configuration (showing disarticulation of the cranidium) became more dominant. This study indicates that gradual transition of ontogenetic moulting behavior occurred in oryctocephalid trilobites in the early Cambrian.
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http://dx.doi.org/10.7717/peerj.12217DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465991PMC
September 2021

Niraparib exhibits a synergistic anti-tumor effect with PD-L1 blockade by inducing an immune response in ovarian cancer.

J Transl Med 2021 10 7;19(1):415. Epub 2021 Oct 7.

Department of Obstetrics and Gynecology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, 107 Wenhua Xi Road, Jinan, Shandong, China.

Background: Immune checkpoint blockades (ICBs) therapy showed limited efficacy in ovarian cancer management. Increasing evidence indicated that conventional and targeted therapies could affect tumor-associated immune responses and increase the effectiveness of immunotherapy. However, the effects of Niraparib, one of the poly (ADP) ribose polymerase (PARP) inhibitors, on the immune response remains unclear. Delineating the crosstalk between cytotoxic anticancer agents and cancer-associated immunity may lead to more efficient combinatorial strategies.

Methods: Programmed death ligand 1 (PD-L1) expression in human ovarian cancer cells after PARP inhibitors treatment was examined by western blotting (WB) and flow cytometry. The expression of poly ADP-ribose polymerase (PARP1), PD-L1, and CD8 in human ovarian cancer tissues was detected by immunohistochemistry(IHC). The effect of Niraparib and PD-L1 blockade in ovarian cancer progression was investigated in vivo. The changes of immune cells and cytokines in vitro and in vivo were detected by flow cytometry and enzyme-linked immunosorbent assay (ELISA). Changes of cGAS/STING signal pathway after Niraparib treatment were determined by WB, ELISA.

Results: Niraparib upregulated membrane PD-L1 and total PD-L1 expression in ovarian cancer cells and had a synergistic effect with PD-L1 blockade in vivo. In clinical patient samples, Niraparib augmented cytotoxic CD8T cell proportion and function. In vivo and vitro, Niraparib can also increase the proportion of T cells and combined with PD-L1 blockade could further enhance the effect. Besides, Niraparib activated the cGAS-STING pathway, increasing the levels of cytokines such as CCL5 and CXCL10, which played a vital role in augmenting the infiltration and activation of cytotoxic T cells.

Conclusions: Niraparib could modulate the immune response via the activation of the cGAS/STING pathway, and combination with PD-L1 blockade could further enhance the effect. These results provide a sound theoretical basis for clinical treatment.
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http://dx.doi.org/10.1186/s12967-021-03073-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8499579PMC
October 2021

Role of endoscopic ultrasonography for differential diagnosis of upper gastrointestinal submucosal lesions.

BMC Gastroenterol 2021 Oct 7;21(1):365. Epub 2021 Oct 7.

Department of Gastroenterology, Hunan Key Laboratory of Non-Resolving Inflammation and Cancer, The Third Xiangya Hospital of Central South University, 138 Tongzi Road, Changsha, 410013, Hunan, People's Republic of China.

Objective: To determine the accuracy of endoscopic ultrasonography (EUS) in the diagnosis of upper gastrointestinal submucosal lesions (SMLs).

Methods: This was a retrospective study involving patients diagnosed with SMLs using EUS and confirmed by histopathology from November 2014 to December 2020 at The Third Xiangya Hospital of Central South University.

Results: A total of 231 patients with SMLs were examined by EUS. Histologically, 107 lesions were stromal tumors, and 75 lesions were leiomyomas. Stromal tumors were mainly located in the stomach (89.7%), and leiomyomas were predominantly seen in the esophagus (69.3%). The diagnostic accuracy of EUS for stromal tumors and leiomyomas was 80.4% and 68.0%, respectively. The diagnostic accuracy was highest for lesions located in the muscularis mucosa. The mean diameter of stromal tumors measured using EUS was significantly larger than that of leiomyomas (21.89 mm vs. 12.35 mm, p < 0.001). Stromal tumors and leiomyomas originated mainly from the muscularis propria (94.4%) and the muscularis mucosa (56.0%), respectively. Compared with the very low-risk and low-risk groups of stromal tumors according to the National Institute of Health guidelines, the intermediate-risk and high-risk groups were more likely to have a lesion > 3 cm (p < 0.001) and a surface ulcer (p < 0.01) identified by EUS.

Conclusions: EUS has good diagnostic value for the diagnosis of upper gastrointestinal SMLs based on the lesion size and the muscle layer of origin. The diagnostic accuracy of EUS lesions is related to the origin, and the diagnostic accuracy is greatest in the mucosal muscularis layer. Stromal tumors > 3 cm and a surface ulcer on EUS are likely to be intermediate or high risk for invasion.
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http://dx.doi.org/10.1186/s12876-021-01945-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495965PMC
October 2021

Age-associated differences in sleep duration in the US population: potential effects of disease burden.

Sleep Med 2021 Nov 20;87:168-173. Epub 2021 Sep 20.

Department of Psychiatry and Behavioral Sciences, Miller School of Medicine, University of Miami, Miami, USA; Department of Neurology, Miller School of Medicine, University of Miami, Miami, USA.

Objectives: We contrasted the relative risks (RR) of short [<7 h] and long [>8 h] sleep experienced by middle-aged (45-64 years) and older (≥65 years) adults, compared with young adults (20-44 years).

Methods: We utilized NHANES data (2005-2016), capturing sociodemographic, socioeconomic, and health-related data among US adults.

Results: The Relative Risk (RR) of short sleep between young and middle-aged adults did not differ [RR = 1.02, NS]. However, the RR of short sleep was significantly reduced among older participants [RR = 0.81, p < 0.01]. Middle-aged adults had significantly lower RR of long sleep [RR = 0.80, p < 0.01], whereas older adults had significantly greater RR of long sleep [RR = 1.41, p < 0.01]. Compared with young adults, older adults with or without increased disease burden had significantly lower RR of short sleep [RR = 0.81, p < 0.01 and RR = 0.80, p < 0.01], respectively. However, for middle-aged adults, the RR of short sleep did not differ whether they reported a greater disease burden. Relative to young adults, older adults with or without disease burden had higher RRs of long sleep [RR = 1.39, p < 0.01] and [RR = 1.45, p < 0.01], respectively. For middle-aged adults without disease burden, the RR of long sleep was lower than among young adults [RR = 0.72, p < 0.01].

Conclusions: Compared with young adults, older adults were not at increased risk for short sleep. Rather, they reported longer sleep time regardless of the presence of disease burden. Future studies should investigate longitudinal effects of aging on objective sleep time, with or without common diseases.
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http://dx.doi.org/10.1016/j.sleep.2021.09.004DOI Listing
November 2021
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