Publications by authors named "Meng Liang"

268 Publications

Van der Waals epitaxy of nearly single-crystalline nitride films on amorphous graphene-glass wafer.

Sci Adv 2021 Jul 30;7(31). Epub 2021 Jul 30.

Research and Development Center for Semiconductor Lighting Technology, Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China.

Van der Waals epitaxy provides a fertile playground for the monolithic integration of various materials for advanced electronics and optoelectronics. Here, a previously unidentified nanorod-assisted van der Waals epitaxy is developed and nearly single-crystalline GaN films are first grown on amorphous silica glass substrates using a graphene interfacial layer. The epitaxial GaN-based light-emitting diode structures, with a record internal quantum efficiency, can be readily lifted off, becoming large-size flexible devices. Without the effects of the potential field from a single-crystalline substrate, we expect this approach to be equally applicable for high-quality growth of nitrides on arbitrary substrates. Our work provides a revolutionary technology for the growth of high-quality semiconductors, thus enabling the hetero-integration of highly mismatched material systems.
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http://dx.doi.org/10.1126/sciadv.abf5011DOI Listing
July 2021

Histogram models based on intravoxel incoherent motion diffusion-weighted imaging to predict nodal staging of rectal cancer.

Eur J Radiol 2021 Jul 20;142:109869. Epub 2021 Jul 20.

Department of Diagnostic Radiology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College. No.17, Panjiayuan Nanli, Chaoyang District, Beijing 100021, China. Electronic address:

Purpose: To develop a model based on histogram parameters derived from intravoxel incoherent motion diffusion-weighted imaging (IVIM-DWI) for predicting the nodal staging of rectal cancer (RC).

Material And Methods: A total of 95 RC patients who underwent direct surgical resection were enrolled in this prospective study. The nodal staging on conventional magnetic resonance imaging (MRI) was evaluated according to the short axis diameter and morphological characteristics. Histogram parameters were extracted from apparent diffusion coefficient (ADC), true diffusion coefficient (D), pseudo-diffusion coefficient (D*), and perfusion fraction (f) maps. Multivariate binary logistic regression analysis was conducted to establish models for predicting nodal staging among all patients and those underestimated on conventional MRI.

Results: The combined model based on multiple maps demonstrated superior diagnostic performance to single map models, with an area under the receiver operating characteristic curve (AUC), sensitivity, specificity, and accuracy of 0.959, 94.3%, 88.3%, and 90.5%, respectively. The AUC of the combined model was significantly higher than that of the conventional nodal staging (P < 0.001). Additionally, 85.0% of the underestimated patients had suspicious lymph nodes with 5-8 mm short-axis diameter. The histogram model for these subgroups of patients showed good diagnostic efficacy with an AUC, sensitivity, specificity, and accuracy of 0.890, 100%, 75%, and 80.5%.

Conclusion: The histogram model based on IVIM-DWI could improve the diagnostic performance of nodal staging of RC. In addition, histogram parameters of IVIM-DWI may help to reduce the uncertainty of nodal staging in underestimated patients on conventional MRI.
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http://dx.doi.org/10.1016/j.ejrad.2021.109869DOI Listing
July 2021

Enhanced Information Flow From Cerebellum to Secondary Visual Cortices Leads to Better Surgery Outcome in Degenerative Cervical Myelopathy Patients: A Stochastic Dynamic Causal Modeling Study With Functional Magnetic Resonance Imaging.

Front Hum Neurosci 2021 23;15:632829. Epub 2021 Jun 23.

Department of Orthopedics Surgery, Tianjin Medical University General Hospital, Tianjin, China.

Degenerative cervical myelopathy (DCM) damages the spinal cord, resulting in long-term neurological impairment including motor and visual deficits. Given that visual feedback is crucial in guiding movements, the visual disorder may be a cause of motor deficits in patients with DCM. It has been shown that increased functional connectivity between secondary visual cortices and cerebellum, which are functionally related to the visually guided movements, was correlated with motor function in patients with DCM. One possible explanation is that the information integration between these regions was increased to compensate for impaired visual acuity in patients with DCM and resulted in better visual feedback during motor function. However, direct evidence supporting this hypothesis is lacking. To test this hypothesis and explore in more detail the information flow within the "visual-cerebellum" system, we measured the effective connectivity (EC) among the "visual-cerebellum" system dynamic causal modeling and then tested the relationship between the EC and visual ability in patients with DCM. Furthermore, the multivariate pattern analysis was performed to detect the relationship between the pattern of EC and motor function in patients with DCM. We found (1) significant increases of the bidirectional connections between bilateral secondary visual cortices and cerebellum were observed in patients with DCM; (2) the increased self-connection of the cerebellum was positively correlated with the impaired visual acuity in patients; (3) the amplitude of effectivity from the cerebellum to secondary visual cortices was positively correlated with better visual recovery following spinal cord decompression surgery; and (4) the pattern of EC among the visual-cerebellum system could be used to predict the pre-operative motor function. In conclusion, this study provided direct evidence that the increased information integration within the "visual-cerebellum" system compensated for visual impairments, which might have importance for sustaining better motor function in patients with DCM.
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http://dx.doi.org/10.3389/fnhum.2021.632829DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261284PMC
June 2021

Desire for Success Awakens: Proof of Competence Restoration in a Non-competitive Environment.

Front Neurosci 2021 21;15:698777. Epub 2021 Jun 21.

School of Business and Management, Shanghai International Studies University, Shanghai, China.

Pioneering studies reported that individuals who worked on a highly difficult task and experienced competence frustration beforehand would activate a restorative process and show enhanced autonomous motivation in a subsequent irrelevant activity. In this follow-up study, we explored the effect of prior competition outcome on one's autonomous motivation in a subsequent non-competitive environment. According to our experimental manipulation, participants were randomly assigned to two treatment groups (a winning group and a losing group) and a control group. The experiment lasted for three sessions. Participants in the control group completed a single-player stop-watch (SW) task all along, while those in both treatment groups worked on a competitive SW task and competed for monetary rewards during Session 2 only. Electrophysiological data in Session 1 serve as the baseline and measure one's trait-level autonomous motivation towards the SW game. For participants in the losing group, more pronounced difference wave of feedback-related negativity was observed in Session 3 compared with Session 1, suggesting enhanced autonomous motivation in Session 3. Such a pattern was observed in neither the winning group nor the control group. These results suggested that failure in a prior competition would activate one's competence restoration in a subsequent non-competitive environment. Task difficulty and social competition are varied sources of competence frustration. Thus, our findings advanced understanding of the competence restorative process and helped clarify the dynamics between competition and human motivation.
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http://dx.doi.org/10.3389/fnins.2021.698777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256259PMC
June 2021

miR-137/ERRα axis mediates chemoresistance of nasopharyngeal carcinoma cells.

J Cell Commun Signal 2021 Jul 1. Epub 2021 Jul 1.

Department of Otolaryngology, Shenzhen Nanshan People's Hospital and The 6Th Affiliated Hospital of Shenzhen University Health Science Center, No.89 Taoyuan Road, Nanshan District, Shenzhen City, 518000, Guangdong Province, People's Republic of China.

Nasopharyngeal carcinoma (NPC) is the most common malignant tumor of the head and neck region and is characterized by an increased risk of developing chemoresistance after treatment. The present study demonstrated that estrogen-related receptor α (ERRα) was upregulated in cisplatin- and fluorouracil-resistant NPC cells. In addition, ERRα knockdown or treatment of cells with the ERRα inverse agonist XCT-790 attenuated the chemoresistance of NPC cells. Mechanistically, the increased expression of ERRα in chemoresistant cells was associated with enhanced mRNA stability. Bioinformatics analysis for screening microRNAs (miRs) regulating the expression of ERRα revealed that miR-137 was downregulated in chemoresistant NPC cells. Additionally, transfection of cells with miR-137 mimics reduced ERRα mRNA stability and increased the chemosensitivity of NPC cells. Furthermore, ERRα knockdown reduced glucose consumption, and lactate and ATP production rates in chemoresistant cells. The aforementioned findings suggested that the miR-137/ERRα-mediated metabolic programming could be involved in the chemoresistance of NPC cells.
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http://dx.doi.org/10.1007/s12079-021-00634-2DOI Listing
July 2021

Occult primary white matter impairment in Leber hereditary optic neuropathy.

Eur J Neurol 2021 Jun 24. Epub 2021 Jun 24.

Department of Radiology & Tianjin Key Lab of Functional Imaging, Tianjin Medical University General Hospital, Tianjin, China.

Background And Purpose: Leber hereditary optic neuropathy (LHON) is a disease maternally inherited from mitochondria that predominantly impairs the retinal ganglion cells and their axons. To identify whether occult brain white matter (WM) impairment is involved, a voxel-based analysis (VBA) of diffusion metrics was carried out in LHON patients with normal-appearing brain parenchyma.

Methods: Fifty-four symptomatic LHON patients (including 22 acute LHON with vision loss for ≤12 months, and 32 chronic LHON) without any visible brain lesions and 36 healthy controls (HCs) were enrolled in this study. VBA was applied to quantify the WM microstructural changes of LHON patients. Finally, the associations of the severity of WM impairment with disease duration and ophthalmologic deficits were assessed.

Results: Compared with the HCs, the average retinal nerve fiber layer (RNFL) thickness was significantly reduced in patients with chronic LHON, whereas it was increased in patients with acute LHON (p < 0.05, corrected). VBA identified significantly decreased fractional anisotropy widely in WM in both the acute and chronic LHON patients, including the left anterior thalamic radiation and superior longitudinal fasciculus, and bilateral corticospinal tract, dentate nuclei, inferior longitudinal fasciculus, forceps major, and optic radiation (OR; p < 0.05, corrected). The integrity of most WM structures (except for the OR) was correlated with neither disease duration nor RNFL thickness (p > 0.05, corrected).

Conclusions: Occult primary impairment of widespread brain WM is present in LHON patients. The coexisting primary and secondary WM impairment may jointly contribute to the pathological process of LHON.
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http://dx.doi.org/10.1111/ene.14995DOI Listing
June 2021

Dynamic compensation control strategy of DC bus voltage based on residual generator.

Sci Prog 2021 Apr-Jun;104(2):368504211026284

State Grid Hebei Electric Power Research Institute, Shijiazhuang, China.

Aiming at the problem of bus voltage control in DC microgrid, a dynamic compensation control strategy based on a residual generator is designed to complete the voltage compensation of DC-DC converter. Firstly, based on the DC microgrid system architecture, the bus voltage fluctuations are analyzed theoretically, and then the DC-DC converter state-space mathematical models of the DC microgrid system are established to obtain the input-output relationship of the control system. Based on the theory of double coprime decomposition and Youla parameterization stable controller, the proposed control architecture based on the residual generator is obtained, and the output value generated by the current disturbance is compensated in reverse by applying model matching theory. The voltage loop compensation controller () is obtained by the linear matrix inequality method (LMI), and the current loop compensation controller () is designed according to the dynamic structure diagram of the DC-DC converter. Hardware-in-the-loop simulation (HILS) results show that the architecture can improve the dynamic performance of the DC-DC converter without changing the original system structure parameters, and suppress the DC bus voltage fluctuations caused by load switching, power fluctuations, and AC-side load imbalances, and enhance the robustness of the system.
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http://dx.doi.org/10.1177/00368504211026284DOI Listing
June 2021

Value of multiple models of diffusion-weighted imaging for improving the nodal staging of preoperatively node-negative rectal cancer.

Abdom Radiol (NY) 2021 Jun 14. Epub 2021 Jun 14.

Department of Diagnostic Radiology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No.17, Panjiayuan Nanli, Chaoyang District, Beijing, 100021, China.

Objective: To investigate the parameters of multiple diffusion-weighted imaging (DWI) models for improving nodal staging of preoperatively node-negative rectal cancer.

Materials And Methods: A total of 74 rectal cancer patients without suspected metastatic lymph nodes on conventional MRI who underwent direct surgical resection between November 2018 and January 2020 were enrolled in this prospective study. DWI parameters of mono-exponential model (ADC), intravoxel incoherent motion (D, D* and f), stretched exponential model (DDC and α), and diffusion kurtosis imaging (MD and MK) within the whole tumor were measured to predict the nodal staging in rectal cancer patients.

Results: The D*, DDC, and MK values were significantly different in patients with pN0 and pN1-2 (all P < 0.001). The D*, DDC, and MK showed good diagnostic performance with the area under the receiver operating characteristic (AUC) of 0.788, 0.827 and 0.799. Multivariate analysis indicated D* (odds ratio, OR = 1.163, P = 0.003) and DDC (OR = 0.007, P = 0.019) as significant predictors of nodal staging. The combination of DDC and D* demonstrated superior diagnostic performance with the AUC, sensitivity, specificity and accuracy of 0.872, 0.800, 0.932 and 0.878, respectively.

Conclusion: Multiple functional DWI parameters were potential to identify the rectal cancer patients with micro-nodal involvement for accurate treatment.
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http://dx.doi.org/10.1007/s00261-021-03125-5DOI Listing
June 2021

Integrated study of circRNA, lncRNA, miRNA, and mRNA networks in mediating the effects of testicular heat exposure.

Cell Tissue Res 2021 May 20. Epub 2021 May 20.

School of Life Science, Bengbu Medical College, Bengbu, Anhui, China.

The World Health Organization has recognized that testicular function is temperature dependent. Testicular heat exposure caused by occupational factors, lifestyle, and clinical diseases can lead to different degrees of reproductive problems. The aim of this study was to reveal the transcriptional regulatory network and its potential crucial roles in mediating the effects of testicular heat exposure. Testicular tissue was collected from a group of mice subjected to scrotal heat exposure as well as a control group. RNA was isolated from both groups and used for high-throughput sequencing. Using differential transcriptome expression analysis, 172 circRNAs, 279 miRNAs, 465 lncRNAs, and 2721 mRNAs were identified as significantly differentially expressed in mouse testicular tissue after heat exposure compared with the control group. Through Gene Ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, differentially expressed lncRNAs and mRNAs were found to have potentially important functions in meiotic cell cycle (GO:0051321), cytoplasm (GO:0005737), membrane raft (GO:0045121), MAPK signaling (mmu04010), purine metabolism (mmu00230), and homologous recombination (mmu03440). Some of the most upregulated and downregulated lncRNAs and circRNAs were predicted to be associated with numerous miRNAs and mRNAs through competing endogenous RNA regulatory network analysis, which were validated with molecular biology experiments. This research provides high-throughput sequencing data of a testicular heat exposure model and lays the foundation for further study on circRNAs, miRNAs, and lncRNAs that are involved in male reproductive diseases related to elevated testicular temperature.
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http://dx.doi.org/10.1007/s00441-021-03474-zDOI Listing
May 2021

Preoperative volumetric synthetic magnetic resonance imaging of the primary tumor for a more accurate prediction of lymph node metastasis in rectal cancer.

Quant Imaging Med Surg 2021 May;11(5):1805-1816

Department of Diagnostic Radiology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: An accurate assessment of lymph node (LN) status in patients with rectal cancer is important for treatment planning and an essential factor for predicting local recurrence and overall survival. In this study, we explored the potential value of histogram parameters of synthetic magnetic resonance imaging (SyMRI) in predicting LN metastasis in rectal cancer and compared their predictive performance with traditional morphological characteristics and chemical shift effect (CSE).

Methods: A total of 70 patients with pathologically proven rectal adenocarcinoma who received direct surgical resection were enrolled in this prospective study. Preoperative rectal MRI, including SyMRI, were performed, and morphological characteristics and CSE of LN were assessed. Histogram parameters were extracted on a T1 map, T2 map, and proton density (PD) map, including mean, variance, maximum, minimum, 10th percentile, median, 90th percentile, energy, kurtosis, entropy, and skewness. Receiver operating characteristic (ROC) curves were used to explore their predictive performance for assessing LN status.

Results: Significant differences in the energy of the T1, T2, and PD maps were observed between LN-negative and LN-positive groups [all P<0.001; the area under the ROC curve (AUC) was 0.838, 0.858, and 0.823, respectively]. The maximum and kurtosis of the T2 map, maximum, and variance of PD map could also predict LN metastasis with moderate diagnostic power (P=0.032, 0.045, 0.016, and 0.047, respectively). Energy of the T1 map [odds ratio (OR) =1.683, 95% confidence interval (CI): 1.207-2.346, P=0.002] and extramural venous invasion on MRI (mrEMVI) (OR =10.853, 95% CI: 2.339-50.364, P=0.002) were significant predictors of LN metastasis. Moreover, the T1 map energy significantly improved the predictive performance compared to morphological features and CSE (P=0.0002 and 0.0485).

Conclusions: The histogram parameters derived from SyMRI of the primary tumor were associated with LN metastasis in rectal cancer and could significantly improve the predictive performance compared with morphological features and CSE.
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http://dx.doi.org/10.21037/qims-20-659DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047345PMC
May 2021

Prediction of false-negative extramural venous invasion in patients with rectal cancer using multiple mathematical models of diffusion-weighted imaging.

Eur J Radiol 2021 Jun 22;139:109731. Epub 2021 Apr 22.

Department of Diagnostic Radiology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College. No.17, Panjiayuan Nanli, Chaoyang District, Beijing 100021, China. Electronic address:

Purpose: To investigate the parameters from mono-exponential, stretched-exponential, and intravoxel incoherent motion diffusion-weighted imaging (DWI) models for evaluating false-negative extramural venous invasion (EMVI) on conventional magnetic resonance imaging (MRI) in rectal cancer patients.

Material And Methods: Seventy-two rectal cancer patients with negative EMVI on conventional MRI who underwent direct surgical resection were enrolled in this prospective study. The apparent diffusion coefficient (ADC), true diffusion coefficient (D), pseudo-diffusion coefficient (D*), perfusion fraction (f), distributed diffusion coefficient (DDC), and water molecular diffusion heterogeneity index (α) values within the whole tumor were obtained to identify the patients with false-negative EMVI. Receiver operating characteristic (ROC) curves were applied to evaluate the diagnostic performance. Multivariate binary logistic regression analysis was conducted to determine the independent risk factors.

Results: The DDC, D*, f, and α values were significantly different in the EMVI-positive and EMVI-negative groups (P =  0.018, and P <  0.001, respectively). The D*, f, and α values demonstrated good diagnostic performance with area under the ROC curve (AUC) of 0.861, 0.824, and 0.854, respectively. The combined model, including D*, α, and tumor location, proved superior diagnostic performance with the AUC, sensitivity, specificity, and accuracy of 0.971, 0.917, 0.967, and 0.931, respectively. The AUC of the combined model was significantly higher than that of the D*, f, and DDC (P = 0.004, 0.045, and 0.002, respectively).

Conclusion: Multi-b-value DWI may be a potential tool for identifying micro-EMVI in rectal cancer. The combination of DWI parameters and tumor location leads to superior diagnostic performance.
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http://dx.doi.org/10.1016/j.ejrad.2021.109731DOI Listing
June 2021

Mindfulness-based interventions for social anxiety disorder: A systematic review and meta-analysis.

Psychiatry Res 2021 Jun 20;300:113935. Epub 2021 Apr 20.

Department of Medical Psychology, School of Mental Health and Psychological Science, Anhui Medical University, Hefei, China. Electronic address:

Various psychiatric disorders are treated with mindfulness-based interventions (MBIs), although the efficacy of MBIs in patients with social anxiety disorder (SAD) is unclear. In this meta-analysis, we investigated the efficacy of MBIs on SAD symptoms. Systematic searches were performed in various databases, and 11 eligible randomized controlled trials (RCTs) and 5 single-arm trials were identified. The between-groups analysis of the 11 RCTs showed that Hedges' g = 0.00, while the within-group analysis showed a large pre-post effect size (g = 1.20).MBIs were superior to the no-treatment comparator (g = 0.89), equivalent to specific active treatment (g = -0.19), and less effective than evidence-based treatment (i.e., cognitive behavioral therapies) (g = -0.29).MBIs significantly alleviated depressive symptoms and improved mindfulness, quality of life, and self-compassion. Meta-regression analysis showed a dose-response relationship between the alleviation of SAD symptoms and the duration of the MBIs (β = 0.659). Follow-up analysis showed that the effects of MBIs on SAD persisted for 12 months (g = 0.231). An analysis of the 5 single-arm trials found that MBIs had a medium effect in alleviating SAD symptoms (g = 0.48). Future research is needed regarding the design of large RCTs of MBIs on SAD patients.
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http://dx.doi.org/10.1016/j.psychres.2021.113935DOI Listing
June 2021

Intracellular enzyme-powered DNA circuit with a tunable amplifier for miRNA imaging.

Chem Commun (Camb) 2021 Apr 22;57(31):3753-3756. Epub 2021 Mar 22.

Institute of Translational Medicine, Department of Otolaryngology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Health Science Center, Shenzhen 518035, China.

We describe an intracellular enzyme-powered DNA circuit probe with a tunable amplifier for sensitive and selective detection of miRNA. This approach has been successfully applied for in situ miRNA-21 fluorescence imaging in live cells. Also, we used chemicals to elevate the APE1 expression level rendering a tunable amplification strength for more flexible imaging applications.
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http://dx.doi.org/10.1039/d1cc00536gDOI Listing
April 2021

Graphene-Nanorod Enhanced Quasi-Van Der Waals Epitaxy for High Indium Composition Nitride Films.

Small 2021 May 31;17(19):e2100098. Epub 2021 Mar 31.

Research and Development Center for Semiconductor Lighting Technology, Institute of Semiconductors, Chinese Academy of Sciences, Beijing, 100083, China.

The nitride films with high indium (In) composition play a crucial role in the fabrication of In-rich InGaN-based optoelectronic devices. However, a major limitation is In incorporation requiring a low temperature during growth at the expense of nitride dissociation. Here, to overcome this limitation, a strain-modulated growth method, namely the graphene (Gr)-nanorod (NR) enhanced quasi-van der Waals epitaxy, is proposed to increase the In composition in InGaN alloy. The lattice transparency of Gr enables constraint of in-plane orientation of nitride film and epitaxial relationships at the heterointerface. The Gr interlayer together with NRs buffer layer substantially reduces the stress of the GaN film by 74.4%, from 0.9 to 0.23 GPa, and thus increases the In incorporation by 30.7%. The first principles calculations confirm that the release of strain accounts for the dramatic improvement. The photoluminescence peak of multiple quantum wells shifts from 461 to 497 nm and the functionally small-sized cyan light-emitting diodes of 7 × 9 mil are demonstrated. These findings provide an efficient approach for the growth of In-rich InGaN film and extend the applications of nitrides in advanced optoelectronic, photovoltaic, and thermoelectric devices.
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http://dx.doi.org/10.1002/smll.202100098DOI Listing
May 2021

Abnormal large-scale structural rich club organization in Leber's hereditary optic neuropathy.

Neuroimage Clin 2021 8;30:102619. Epub 2021 Mar 8.

Department of Radiology & Tianjin Key Lab of Functional Imaging, Tianjin Medical University General Hospital, Tianjin 300052, China. Electronic address:

Objective: The purpose of this study was to investigate whether the large-scale structural rich club organization was abnormal in patients with Leber's hereditary optic neuropathy (LHON) using diffusion tensor imaging (DTI), and the associations among disrupted brain structural connectivity, disease duration, and neuro-ophthalmological impairment.

Methods: Nineteen acute, 34 chronic LHON patients, and 36 healthy controls (HC) underwent DTI and neuro-ophthalmological measurements. The brain structural network and rich club organization were constructed based on deterministic fiber tracking at the individual level. Then intergroup differences among the acute, chronic LHON patients and healthy controls (HC) in three types of structural connections, including rich club, feeder, and local ones, were compared. Network-based Statistics (NBS) was also used to test the intergroup connectivity differences for each fiber. Several linear and nonlinear curve fit models were applied to explore the associations among large-scale brain structural connectivity, disease duration, and neuro-ophthalmological metrics.

Results: Compared to the HC, both the acute and chronic LHON patients had consistently significantly lower fractional anisotropy (FA) and higher radial diffusion (RD) for feeder connections (p < 0.05, FDR correction). Acute LHON patients had significantly lower FA and higher RD for local connections (p < 0.05, FDR correction). There was no significant difference in large-scale brain structural connectivity between acute and chronic LHON (p > 0.05, FDR correction). NBS also identified reduced FA of three feeder connections and five local ones linking visual, auditory, and basal ganglia areas in LHON patients (p < 0.05, FDR correction). No structural connections showed linear or nonlinear association with either disease duration or neuro-ophthalmological indicators (p > 0.05, FDR correction). A significant negative correlation was shown between the retinal nerve fiber layer (RNFL) thickness and disease duration (p < 0.05, FDR correction).

Conclusions: Abnormal rich club organization of the structural network was identified in both the acute and chronic LHON. Furthermore, our findings suggest the coexistence of both primary and secondary connectivity damage in the LHON.
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http://dx.doi.org/10.1016/j.nicl.2021.102619DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010853PMC
July 2021

Prediction of pathological prognostic factors of rectal cancer by relaxation maps from synthetic magnetic resonance imaging.

Eur J Radiol 2021 May 15;138:109658. Epub 2021 Mar 15.

Department of Diagnostic Radiology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No.17, Panjiayuan Nanli, Chaoyang District, Beijing, 100021, China. Electronic address:

Purpose: To explore the feasibility of relaxation maps from synthetic MRI for predicting pathological prognostic factors of rectal cancer (RC) and to compare the predictive performance of quantitative values and conventional subjective evaluation.

Material And Methods: A total of 94 patients with pathologically proven RC who underwent direct surgical resection were enrolled in this prospective study. Preoperative rectal MRI including synthetic MRI was performed. The mean T, T, and PD value of the whole tumor was obtained to preoperatively assess the pathological T stage, N stage, extramural venous invasion (EMVI), differentiation, and perineural invasion. Receiver operating characteristic curves were used to explore the predictive performance for assessing the prognostic factors. The T stage, N stage and EMVI status on conventional T2WI were evaluated and compared with the quantitative values.

Results: The T value decreased significantly in patients with positive perineural invasion, lymph node metastasis (LNM), EMVI, and higher T stage RC (p =  0.007 and < 0.001). The T value of LNM and EMVI positive groups was significantly lower than those of the negative groups (p =  0.034 and 0.011). For predicting N stage and EMVI, the T value demonstrated good performance with an AUC of 0.883 (95 % confidence interval, CI, 0.801-0.940) and 0.821 (95 % CI, 0.729-0.893); the T value was superior to the T value and subjective evaluation of radiologists (all p < 0.05).

Conclusion: Synthetic MRI is a promising tool for noninvasive evaluation of prognostic factors of RC by generating relaxation maps.
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http://dx.doi.org/10.1016/j.ejrad.2021.109658DOI Listing
May 2021

Feedforward and feedback pathways of nociceptive and tactile processing in human somatosensory system: A study of dynamic causal modeling of fMRI data.

Neuroimage 2021 07 17;234:117957. Epub 2021 Mar 17.

School of Medical Imaging and Tianjin Key Laboratory of Functional Imaging, Tianjin Medical University, Tianjin, China. Electronic address:

Nociceptive and tactile information is processed in the somatosensory system via reciprocal (i.e., feedforward and feedback) projections between the thalamus, the primary (S1) and secondary (S2) somatosensory cortices. The exact hierarchy of nociceptive and tactile information processing within this 'thalamus-S1-S2' network and whether the processing hierarchy differs between the two somatosensory submodalities remains unclear. In particular, two questions related to the ascending and descending pathways have not been addressed. For the ascending pathways, whether tactile or nociceptive information is processed in parallel (i.e., 'thalamus-S1' and 'thalamus-S2') or in serial (i.e., 'thalamus-S1-S2') remains controversial. For the descending pathways, how corticothalamic feedback regulates nociceptive and tactile processing also remains elusive. Here, we aimed to investigate the hierarchical organization for the processing of nociceptive and tactile information in the 'thalamus-S1-S2' network using dynamic causal modeling (DCM) combined with high-temporal-resolution fMRI. We found that, for both nociceptive and tactile information processing, both S1 and S2 received inputs from thalamus, indicating a parallel structure of ascending pathways for nociceptive and tactile information processing. Furthermore, we observed distinct corticothalamic feedback regulations from S1 and S2, showing that S1 generally exerts inhibitory feedback regulation independent of external stimulation whereas S2 provides additional inhibition to the thalamic activity during nociceptive and tactile information processing in humans. These findings revealed that nociceptive and tactile information processing have similar hierarchical organization within the somatosensory system in the human brain.
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http://dx.doi.org/10.1016/j.neuroimage.2021.117957DOI Listing
July 2021

Preoperative evaluation of extramural venous invasion in rectal cancer using radiomics analysis of relaxation maps from synthetic MRI.

Abdom Radiol (NY) 2021 08 20;46(8):3815-3825. Epub 2021 Mar 20.

Department of Diagnostic Radiology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Objective: To establish a radiomics nomogram based on relaxation maps for predicting the extramural venous invasion (EMVI) of rectal cancer (RC) and compare the diagnostic efficacy of the nomogram and subjective assessment by radiologists.

Material And Methods: Among 94 RC patients receiving direct surgical resection, 65 were randomly allocated to the training cohort and 29 to the validation cohort. Radiomics features were extracted from synthetic magnetic resonance imaging including T1, T2, and proton density (PD) maps. The least absolute shrinkage and selection operator methods were used for dimension reduction, feature selection, and radiomics model building. Multivariable logistic regression analysis was used for nomogram development. The performance of the nomogram was assessed with respect to its calibration, receiver operating characteristics (ROC) curve, and decision curve analysis.

Results: The radiomics model demonstrated good predictive efficacy for EMVI, with an area under the ROC curve (AUC), sensitivity, and specificity of 0.912 (95% confidence interval (CI), 0.837-0.986), 0.824, and 0.875 in the training cohort and 0.877 (95% CI 0.751-1.000), 0.833, and 0.826 in the validation cohort. The nomogram had good diagnostic performance, with AUCs of 0.925 (95% CI 0.862-0.988) and 0.899 (95% CI 0.782-1.000) in the training and validation cohort. Furthermore, the radiomics signature showed better diagnostic efficiency than the subjective assessment by both readers (AUC =0.912 vs. 0.732 and 0.763, P = 0.023 and 0.028, respectively).

Conclusion: A radiomics nomogram was developed to preoperatively predict EMVI in RC patients. The application of the radiomics model based on relaxation maps could improve the diagnostic efficacy of EMVI.
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http://dx.doi.org/10.1007/s00261-021-03021-yDOI Listing
August 2021

Brain Gene Expression Pattern Correlated with the Differential Brain Activation by Pain and Touch in Humans.

Cereb Cortex 2021 Jun;31(7):3506-3521

Tianjin Key Laboratory of Functional Imaging, Department of Radiology, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.

Genes involved in pain and touch sensations have been studied extensively, but very few studies have tried to link them with neural activities in the brain. Here, we aimed to identify genes preferentially correlated to painful activation patterns by linking the spatial patterns of gene expression of Allen Human Brain Atlas with the pain-elicited neural responses in the human brain, with a parallel, control analysis for identification of genes preferentially correlated to tactile activation patterns. We identified 1828 genes whose expression patterns preferentially correlated to painful activation patterns and 411 genes whose expression patterns preferentially correlated to tactile activation pattern at the cortical level. In contrast to the enrichment for astrocyte and inhibitory synaptic transmission of genes preferentially correlated to tactile activation, the genes preferentially correlated to painful activation were mainly enriched for neuron and opioid- and addiction-related pathways and showed significant overlap with pain-related genes identified in previous studies. These findings not only provide important evidence for the differential genetic architectures of specific brain activation patterns elicited by painful and tactile stimuli but also validate a new approach to studying pain- and touch-related genes more directly from the perspective of neural responses in the human brain.
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http://dx.doi.org/10.1093/cercor/bhab028DOI Listing
June 2021

Does the beta regularization parameter of bayesian penalized likelihood reconstruction always affect the quantification accuracy and image quality of positron emission tomography computed tomography?

J Appl Clin Med Phys 2021 Mar 8;22(3):224-233. Epub 2021 Mar 8.

Department of Nuclear Medicine, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, P.R. China.

Purpose: This study aims to provide a detailed investigation on the noise penalization factor in Bayesian penalized likelihood (BPL)-based algorithm, with the utilization of partial volume effect correction (PVC), so as to offer the suitable beta value and optimum standardized uptake value (SUV) parameters in clinical practice for small pulmonary nodules.

Methods: A National Electrical Manufacturers Association (NEMA) image-quality phantom was scanned and images were reconstructed using BPL with beta values ranged from 100 to 1000. The recovery coefficient (RC), contrast recovery (CR), and background variability (BV) were measured to assess the quantification accuracy and image quality. In the clinical assessment, lesions were categorized into sub-centimeter (<10 mm, n = 7) group and medium size (10-30 mm, n = 16) group. Signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) were measured to evaluate the image quality and lesion detectability. With PVC was performed, the impact of beta values on SUVs (SUVmax, SUVmean, SUVpeak) of small pulmonary nodules was evaluated. Subjective image analysis was performed by two experienced readers.

Results: With the increasing of beta values, RC, CR, and BV decreased gradually in the phantom work. In the clinical study, SNR and CNR of both groups increased with the beta values (P < 0.001), although the sub-centimeter group showed increases after the beta value reached over 700. In addition, highly significant negative correlations were observed between SUVs and beta values for both lesion-size groups before the PVC (P < 0.001 for all). After the PVC, SUVpeak measured from the sub-centimeter group was no significantly different among different beta values (P = 0.830).

Conclusion: Our study suggests using SUVpeak as the quantification parameter with PVC performed to mitigate the effects of beta regularization. Beta values between 300 and 400 were preferred for pulmonary nodules smaller than 30 mm.
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http://dx.doi.org/10.1002/acm2.13129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7984479PMC
March 2021

Anatomical and functional coupling between the dorsal and ventral attention networks.

Neuroimage 2021 05 27;232:117868. Epub 2021 Feb 27.

Department of Radiology, Tianjin Medical University General Hospital, Anshan Road No 154, Heping District, Tianjin 300052, China; Tianjin Key Lab of Functional Imaging, Tianjin Medical University General Hospital, Tianjin 300052, China. Electronic address:

Studies have indicated that the dorsal attention network (DAN) and the ventral attention network (VAN) functionally interact via several fronto-parietal connector hubs. However, the anatomical connectivity profiles of these connector hubs, and the coupling between the anatomical and functional connectivities of them, are still unknown. In the present study, we found that functional connector hubs anatomically bridged the DAN and VAN based on multimodal magnetic resonance imaging data from the Human Connectome Project (HCP) Consortium and an independent Chinese cohort. The three hubs had unique anatomical connectivity patterns with the attention sub-networks. For each connector hub, the pattern of anatomical connectivity resembled the functional one. Finally, the strength of the anatomical connectivity of these connector hubs was positively associated with the functional connectivity at the group- and individual-levels. Our findings help to better understand the anatomical mechanisms underlying the functional interactions between the DAN and the VAN.
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http://dx.doi.org/10.1016/j.neuroimage.2021.117868DOI Listing
May 2021

Stability test of canonical correlation analysis for studying brain-behavior relationships: The effects of subject-to-variable ratios and correlation strengths.

Hum Brain Mapp 2021 Jun 24;42(8):2374-2392. Epub 2021 Feb 24.

School of Medical Imaging and Tianjin Key Laboratory of Functional Imaging, Tianjin Medical University, Tianjin, China.

Canonical correlation analysis (CCA), a multivariate approach to identifying correlations between two sets of variables, is becoming increasingly popular in neuroimaging studies on brain-behavior relationships. However, the CCA stability in neuroimaging applications has not been systematically investigated. Although it is known that the number of subjects should be greater than the number of variables due to the curse of dimensionality, it is unclear at what subject-to-variable ratios (SVR) and at what correlation strengths the CCA stability can be maintained. Here, we systematically assessed the CCA stability, in the context of investigating the relationship between the brain structural/functional imaging measures and the behavioral measures, by measuring the similarity of the first-mode canonical variables across randomly sampled subgroups of subjects from a large set of 936 healthy subjects. Specifically, we tested how the CCA stability changes with SVR under two different brain-behavior correlation strengths. The same tests were repeated using an independent data set (n = 700) for validation. The results confirmed that both SVR and correlation strength affect greatly the CCA stability-the CCA stability cannot be guaranteed if the SVR is not sufficiently high or the brain-behavior relationship is not sufficiently strong. Based on our quantitative characterization of CCA stability, we provided a practical guideline to help correct interpretation of CCA results and proper applications of CCA in neuroimaging studies on brain-behavior relationships.
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http://dx.doi.org/10.1002/hbm.25373DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8090773PMC
June 2021

Efficacy and safety of Shenyankangfu Tablet, a Chinese patent medicine, for primary glomerulonephritis: A multicenter randomized controlled trial.

J Integr Med 2021 Mar 30;19(2):111-119. Epub 2021 Jan 30.

Department of Nephrology, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin 150040, Heilongjiang Province, China.

Background: Shenyankangfu Tablet (SYKFT) is a Chinese patent medicine that has been used widely to decrease proteinuria and the progression of chronic kidney disease.

Objective: This trial compared the efficacy and safety of SYKFT, for the control of proteinuria in primary glomerulonephritis patients, against the standard drug, losartan potassium.

Design, Setting, Participants And Intervention: This was a multicenter, double-blind, randomized, controlled clinical trial. Primary glomerulonephritis patients, aged 18-70 years, with blood pressure ≤ 140/90 mmHg, estimated glomerular filtration rate (eGFR) ≥ 45 mL/min per 1.73 m, and 24-hour proteinuria level of 0.5-3.0 g, were recruited in 41 hospitals across 19 provinces in China and were randomly divided into five groups: SYKFT, losartan potassium 50 mg or 100 mg, SYKFT plus losartan potassium 50 mg or 100 mg.

Main Outcome Measures: The primary outcome was change in the 24-hour proteinuria level, after 48 weeks of treatment.

Results: A total of 735 participants were enrolled. The percent decline of urine protein quantification in the SYKFT group after 48 weeks was 8.78% ± 2.56% (P = 0.006) more than that in the losartan 50 mg group, which was 0.51% ± 2.54% (P = 1.000) less than that in the losartan 100 mg group. Compared with the losartan potassium 50 mg group, the SYKFT plus losartan potassium 50 mg group had a 13.39% ± 2.49% (P < 0.001) greater reduction in urine protein level. Compared with the losartan potassium 100 mg group, the SYKFT plus losartan potassium 100 mg group had a 9.77% ± 2.52% (P = 0.001) greater reduction in urine protein. With a superiority threshold of 15%, neither was statistically significant. eGFR, serum creatinine and serum albumin from the baseline did not change statistically significant. The average change in TCM syndrome score between the patients who took SYKFT (-3.00 [-6.00, -2.00]) and who did not take SYKFT (-2.00 [-5.00, 0]) was statistically significant (P = 0.003). No obvious adverse reactions were observed in any group.

Conclusion: SYKFT decreased the proteinuria and improved the TCM syndrome scores of primary glomerulonephritis patients, with no change in the rate of decrease in the eGFR. SYKFT plus losartan potassium therapy decreased proteinuria more than losartan potassium therapy alone.

Trial Registration Number: NCT02063100 on ClinicalTrials.gov.
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http://dx.doi.org/10.1016/j.joim.2021.01.009DOI Listing
March 2021

Establishment and validation of an RNA binding protein-associated prognostic model for ovarian cancer.

J Ovarian Res 2021 Feb 7;14(1):27. Epub 2021 Feb 7.

School of Life Science, Bengbu Medical College, Bengbu, 233030, Anhui, People's Republic of China.

Background: Ovarian cancer (OC) is one of the most common gynecological malignant tumors worldwide, with high mortality and a poor prognosis. As the early symptoms of malignant ovarian tumors are not obvious, the cause of the disease is still unclear, and the patients' postoperative quality of life of decreases. Therefore, early diagnosis is a problem requiring an urgent solution.

Methods: We obtained the gene expression profiles of ovarian cancer and normal samples from TCGA and GTEx databases for differential expression analysis. From existing literature reports, we acquired the RNA-binding protein (RBP) list for the human species. Utilizing the online tool Starbase, we analyzed the interaction relationship between RBPs and their target genes and selected the modules of RBP target genes through Cytoscape. Finally, univariate and multivariate Cox regression analyses were used to determine the prognostic RBP signature.

Results: We obtained 527 differentially expressed RBPs, which were involved in many important cellular events, such as RNA splicing, the cell cycle, and so on. We predicted several target genes of RBPs, constructed the interaction network of RBPs and their target genes, and obtained many modules from the Cytoscape analysis. Functional enrichment of RBP target genes also includes these important biological processes. Through Cox regression analysis, OC prognostic RBPs were identified and a 10-RBP model constructed. Further analysis showed that the model has high accuracy and sensitivity in predicting the 3/5-year survival rate.

Conclusions: Our study identified differentially expressed RBPs and their target genes in OC, and the results promote our understanding of the molecular mechanism of ovarian cancer. The current study could develop novel biomarkers for the diagnosis, treatment, and prognosis of OC and provide new ideas and prospects for future clinical research.
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http://dx.doi.org/10.1186/s13048-021-00777-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7869493PMC
February 2021

[Effects of bisphenol A on apoptosis of ovarian preantral follicular granulosa cells and ovarian development in mice].

Nan Fang Yi Ke Da Xue Xue Bao 2021 Jan;41(1):93-99

School of Life Science, Bengbu Medical College, Bengbu 233030, China.

Objective: To investigate the effect of environmental estrogen bisphenol A (BPA) exposure on apoptosis of mouse ovarian preantral follicular granulosa cells and ovarian development and explore the underlying mechanism.

Methods: Mouse ovarian preantral follicular granulosa cells were isolated from female ICR mice at postnatal day (PND) 10 and cultured . The cultured cells were treated with 0, 1, 10, 50, 100, 150, 200 and 500 μmol/L BPA, and the changes in cell proliferation, cell cycle, apoptosis and mitochondrial membrane potential were analyzed with CCK-8 method and flow cytometry. The protein expressions of Bcl-2, Bax, p53 and cyclin D1 in the treated cells were determined with Western blotting. Pregnant ICR mice were treated for a week with BPA at the concentration that produced significant effects on the preantral follicular granulosa cells, and the weight changes of the pregnant mice were recorded. The ovarian tissues of the offspring female mice were weighed at PND 10, 17, 21 and 42 followed by histological observation with HE staining and examination of Bcl-2 mRNA expression level with RT-qPCR.

Results: Compared with the control cells group, the isolated cells exposed to a low concentration of BPA (50 μmol/L) showed a significantly lowered apoptosis rate, increased mitochondrial membrane potential, and enhanced cellular proliferation ( < 0.05). Exposure to a higher BPA concentration at 200 μmol/L obviously enhanced cell apoptosis by reducing the mitochondrial membrane potential and repressed the cell proliferation ( < 0.05). BPA exposure at 50 μmol/L and 200 μmol/L produced opposite effects on the protein expressions of Bcl-2 (P < 0.01), Bax ( < 0.05) and p53 ( < 0.05) in mouse ovarian preantral follicular granulosa cells. BPA exposure at the doses of 10 and 35 mg/kg caused rapid weight increment of the pregnant mice and changes in ovarian index of the offspring female mice. In the offspring female mice, the changes in Bcl-2 mRNA expression in the ovarian tissue showed a similar pattern to that of ovarian index. Exposure of the pregnant mice to a high BPA concentration at 35 mg/kg resulted in accelerated follicular development into antral follicular stage in PND 21 offspring female mice.

Conclusions: BPA can concentration-dependently regulate the function of ovarian preantral follicular granulosa cells in mice and potentially affects both the pregnant mice and the offspring female mice in light of early ovarian development.
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http://dx.doi.org/10.12122/j.issn.1673-4254.2021.01.13DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867475PMC
January 2021

To Reveal or Not to Reveal? Observation of Social Outcomes Facilitates Reward Processing.

Front Neurosci 2020 11;14:579702. Epub 2021 Jan 11.

Laboratory of Applied Brain and Cognitive Sciences, School of Business and Management, Shanghai International Studies University, Shanghai, China.

Motivation is a key topic that comprises considerable theoretical and practical implications, and its study is gaining increasing traction in recent years. Employing both behavioral and neural techniques, previous studies examined the extent to which intrinsic and extrinsic motivations collectively shape individual decision making. Investigations found that both processes play indispensable and interactive roles in choice behavior. However, despite its importance, little is known respecting the role of extrinsic social factors in contributing to individual variations in intrinsic motivation. Toward elucidating the role of extrinsic social factors in motivated decision making, the current study implements the stop watch task, combined with hyper-recording electrophysiological measurements. With the electrophysiological toolkit, our goal is to bring to light how extrinsic social signals impact intrinsic motivation and shape the reward processing over success and failure at the succeeding stage. Empirically, we show that, following social outcome presentation, there is an increased divergent feedback-related negativity (FRN), which reflects the failure/success discrepancy at the outcome stage of choice behavior. In summary, this study demonstrates the saliency of social information in intrinsic motivational processes that underpin success-failure outcomes.
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http://dx.doi.org/10.3389/fnins.2020.579702DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829484PMC
January 2021

Dysregulation of the Sirt5/IDH2 axis contributes to sunitinib resistance in human renal cancer cells.

FEBS Open Bio 2021 03 8;11(3):921-931. Epub 2021 Feb 8.

Department of Computer Tomography, Cangzhou Central Hospital, China.

Sunitinib (Sun), a tyrosine kinase inhibitor of vascular endothelial growth factor receptor, is the standard first-line treatment against advanced clear cell renal cell carcinoma (RCC), but resistance to therapy is inevitable. Reactive oxygen species production is associated with sensitivity to chemotherapy, but the underlying mechanisms are not completely understood. Here, we investigated the mechanisms contributing to Sun resistance using the RCC cell lines ACHN and 786-O. We report that Sun-resistant cells exhibited reduced apoptosis, increased cell viability, increased reactive oxygen species production and disrupted mitochondrial function. Furthermore, chronic Sun treatment resulted in an up-regulation of Sirt5/isocitrate dehydrogenase 2 (IDH2) expression levels. Knockdown of Sirt5/IDH2 impaired mitochondrial function and partially attenuated Sun resistance. Finally, up-regulation of Sirt5 enhanced the expression of IDH2 via modulation of succinylation at K413 and promoted protein stability. In conclusion, dysregulation of Sirt5/IDH2 partially contributes to Sun resistance in RCC cells by affecting antioxidant capacity.
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http://dx.doi.org/10.1002/2211-5463.13090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7931237PMC
March 2021

Neural Correlates of Cognitive Dysfunctions in Cervical Spondylotic Myelopathy Patients: A Resting-State fMRI Study.

Front Neurol 2020 23;11:596795. Epub 2020 Dec 23.

Department of Orthopedics Surgery, Tianjin Medical University General Hospital, Tianjin, China.

Cervical spondylotic myelopathy (CSM) is a common disease of the elderly that is characterized by gait instability, sensorimotor deficits, etc. Recurrent symptoms including memory loss, poor attention, etc. have also been reported in recent studies. However, these have been rarely investigated in CSM patients. To investigate the cognitive deficits and their correlation with brain functional alterations, we conducted resting-state fMRI (rs-fMRI) signal variability. This is a novel indicator in the neuroimaging field for assessing the regional neural activity in CSM patients. Further, to explore the network changes in patients, functional connectivity (FC) and graph theory analyses were performed. Compared with the controls, the signal variabilities were significantly lower in the widespread brain regions especially at the default mode network (DMN), visual network, and somatosensory network. The altered inferior parietal lobule signal variability positively correlated with the cognitive function level. Moreover, the FC and the global efficiency of DMN increased in patients with CSM and positively correlated with the cognitive function level. According to the study results, (1) the cervical spondylotic myelopathy patients exhibited regional neural impairments, which correlated with the severity of cognitive deficits in the DMN brain regions, and (2) the increased FC and global efficiency of DMN can compensate for the regional impairment.
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http://dx.doi.org/10.3389/fneur.2020.596795DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7785814PMC
December 2020

RNAi-mediated knockdown of PFK1 decreases the invasive capability and metastasis of nasopharyngeal carcinoma cell line, CNE-2.

Cell Cycle 2021 01 6;20(2):154-165. Epub 2021 Jan 6.

Department of Otolaryngology Head and Neck Surgery, The 5th Affiliated Hospital of Sun Yat-sen University , Zhuhai, China.

Nasopharyngeal carcinoma (NPC) is the most prevailing malignancy of the head and neck with unique geographic distribution. Southern China has one of the highest incidence rates of NPC in the world. Although radiotherapy and chemotherapy are the most important treatment modalities for NPC, recurrence, and metastasis severely interfere with the survival quality of patients. It is much-needed to find an effective method of NPC treatment with a good prognosis such as gene therapy. PFK1, a key regulatory enzyme of glycolysis, is frequently shown to be amplified and overexpressed in a variety of human cancers. However, the function of PFK1 and molecular mechanism in NPC is elusive. Here, we knockdown PFK1 expression by utilizing DNA vector-based RNA Interference. Western blotting and real-time PCR show that the expression of PFK1 is efficiently down-regulated in both protein and mRNA levels by stable transfection with PFK1 siRNA expression vector. In addition, stable knockdown of PFK1 expression inhibits cell growth, induces apoptosis, decreases the invasive capability and metastasis in the CNE2 human NPC cell line. This present study finds the importance of PFK1 which can be worked as a novel target in NPC treatment and holds great potential to be extended to other malignant cancers.
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http://dx.doi.org/10.1080/15384101.2020.1866279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889105PMC
January 2021
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