Publications by authors named "Manuel Cuenca-Estrella"

100Publications

In vitro activity of olorofim against clinical isolates of Scedosporium species and Lomentospora prolificans using EUCAST and CLSI methodologies.

J Antimicrob Chemother 2020 Dec;75(12):3582-3585

Mycology Reference Laboratory, National Centre for Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.

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December 2020

EUCAST Reference Testing of Rezafungin Susceptibility and Impact of Choice of Plastic Plates.

Antimicrob Agents Chemother 2019 09 23;63(9). Epub 2019 Aug 23.

Mycology Reference Laboratory, National Centre for Microbiology, Instituto de Salud Carlos III, Madrid, Spain.

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September 2019

An introduction to current standards of CARE in invasive fungal disease.

J Antimicrob Chemother 2019 03;74(Suppl 2):ii2

Department of Microbiology and Immunology, KU Leuven, Leuven, Belgium.

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March 2019

Defining standards of CARE for invasive fungal diseases in the ICU.

J Antimicrob Chemother 2019 03;74(Suppl 2):ii9-ii15

Clinical Department of General Internal Medicine, Medical Intensive Care Unit, University Hospitals Leuven, Leuven, Belgium.

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March 2019

In vitro activity of olorofim (F901318) against clinical isolates of cryptic species of Aspergillus by EUCAST and CLSI methodologies.

J Antimicrob Chemother 2019 06;74(6):1586-1590

Mycology Reference Laboratory, National Centre for Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain; Spanish Network for the Research in Infectious Diseases (REIPI RD16/CIII/0004/0003), Instituto de Salud Carlos III, Madrid, Spain.

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June 2019

In vitro activity of APX001A against rare moulds using EUCAST and CLSI methodologies.

J Antimicrob Chemother 2019 05;74(5):1295-1299

Mycology Reference Laboratory, National Centre for Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain; Spanish Network for the Research in Infectious Diseases (RD16/CIII/0004/0003), Instituto de Salud Carlos III, Madrid, Spain.

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May 2019

Endemic Fungal Infection Recommendations for Solid-Organ Transplant Recipients and Donors.

Transplantation 2018 02;102(2S Suppl 2):S52-S59

Spanish National Center for Microbiology, Instituto de Salud Carlos III, Madrid, Spain.

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February 2018

Changes in the epidemiological landscape of invasive mould infections and disease.

J Antimicrob Chemother 2017 03;72(suppl_1):i5-i11

Department of Mycology, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo Km 2, Majadahonda, Madrid, Spain.

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March 2017

Triazole Resistance in Aspergillus Species: An Emerging Problem.

Drugs 2017 Apr;77(6):599-613

Mycology Department, Centro Nacional de Microbiologia, Instituto de Salud Carlos III (ISCIII), Carretera Majadahonda-Pozuelo Km2, Majadahonda, 28220, Madrid, Spain.

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April 2017

Fungemia due to rare opportunistic yeasts: data from a population-based surveillance in Spain.

Med Mycol 2017 Feb 4;55(2):125-136. Epub 2016 Aug 4.

Unit of Infectious Diseases, Hospital Universitario "12 de Octubre," Instituto de Investigación Hospital "12 de Octubre" (i+12), Universidad Complutense, Madrid, Spain.

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February 2017

Triazole Resistance in Aspergillus spp.: A Worldwide Problem?

J Fungi (Basel) 2016 Jul 4;2(3). Epub 2016 Jul 4.

Mycology Reference Laboratory, National Centre for Microbiology, Instituto de Salud Carlos III., Carretera de Majadahonda a Pozuelo Km. 2, Majadahonda, 28220 Madrid, Spain.

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July 2016

Diagnostics and susceptibility testing in Aspergillus.

Future Microbiol 2016 5;11(2):315-28. Epub 2016 Feb 5.

Mycology Reference Laboratory, National Centre for Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain; Spanish Network for the Research in Infectious Diseases (REIPI RD12/0015), Instituto de Salud Carlos III, Madrid, Spain.

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November 2016

In Vitro Activity of ASP2397 against Aspergillus Isolates with or without Acquired Azole Resistance Mechanisms.

Antimicrob Agents Chemother 2016 01 9;60(1):532-6. Epub 2015 Nov 9.

Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Madrid, Spain.

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January 2016

Candida tropicalis bloodstream infection: Incidence, risk factors and outcome in a population-based surveillance.

J Infect 2015 Sep 30;71(3):385-94. Epub 2015 May 30.

Unit of Infectious Diseases, Hospital Universitario "12 de Octubre", Instituto de Investigación Hospital "12 de Octubre" (i+12), Universidad Complutense, Madrid, Spain.

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September 2015

Reply to Lewis et al.

Clin Infect Dis 2015 Apr 26;60(8):1285. Epub 2015 Jan 26.

Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain.

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April 2015

Erratum to: Antifungal susceptibility profile of cryptic species of Aspergillus.

Mycopathologia 2015 Apr;179(3-4):333-4

Mycology Reference Laboratory, National Centre for Microbiology, Spanish Network for the Research in Infectious Diseases (REIPI RD12/0015), Instituto de Salud Carlos III, Majadahonda, Madrid, Spain,

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April 2015

EUCAST testing of Isavuconazole susceptibility in Aspergillus: comparison of results for Inoculum standardization using Conidium counting versus optical density.

Antimicrob Agents Chemother 2014 Nov 18;58(11):6432-6. Epub 2014 Aug 18.

Department of Mycology, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.

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November 2014

Genetic relatedness versus biological compatibility between Aspergillus fumigatus and related species.

J Clin Microbiol 2014 Oct 6;52(10):3707-21. Epub 2014 Aug 6.

Molecular Microbiology Section, Laboratory of Clinical Infectious Diseases, NIAID, NIH, Bethesda, Maryland, USA

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October 2014

Antifungal susceptibility profile of cryptic species of Aspergillus.

Mycopathologia 2014 Dec 28;178(5-6):427-33. Epub 2014 Jun 28.

Mycology Reference Laboratory, National Centre for Microbiology, Spanish Network for the Research in Infectious Diseases (REIPI RD12/0015), Instituto de Salud Carlos III, Majadahonda, Madrid, Spain,

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December 2014

Development and validation of a quantitative real-time PCR assay for the early diagnosis of coccidioidomycosis.

Diagn Microbiol Infect Dis 2014 Jun 20;79(2):214-21. Epub 2014 Feb 20.

California Institute for Medical Research, San Jose, CA, USA; Department of Medicine, Santa Clara Valley Medical Center, San Jose, CA, USA; Department of Medicine, Stanford University, Stanford, CA, USA.

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June 2014

Breakpoints for antifungal agents: an update from EUCAST focussing on echinocandins against Candida spp. and triazoles against Aspergillus spp.

Drug Resist Updat 2013 Dec 27;16(6):81-95. Epub 2014 Jan 27.

Antimicrobial Pharmacodynamics and Therapeutics, Department of Molecular and Clinical Pharmacology, University of Liverpool, Liverpool, United Kingdom.

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December 2013

Impact of therapeutic strategies on the prognosis of candidemia in the ICU.

Crit Care Med 2014 Jun;42(6):1423-32

1Infectious Diseases Department, Hospital Universitari Vall d´Hebron, Medicine Department, Universitat Autònoma de Barcelona, Barcelona, Spain. 2Microbiology Department, Hospital Universitario y Politécnico La Fe, Valencia, Spain. 3Intensive Care Medicine Department, Hospital Universitario Dr. Peset, Valencia, Spain. 4Critical Care and Emergency Department, Hospital Universitario Virgen del Rocío, Seville, Spain. 5Infectious Diseases and Microbiology Unit, Hospital Universitario de Valme, Instituto de Biomedicina de Sevilla (IbiS), Seville, Spain. 6Department of Mycology, Spanish National Center for Microbiology, Instituto de Salud Carlos III, Madrid, Spain.

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June 2014

EUCAST technical note on Candida and micafungin, anidulafungin and fluconazole.

Mycoses 2014 Jun 13;57(6):377-9. Epub 2014 Jan 13.

Unit of Mycology, Department of Microbiology & Infection Control, Statens Serum Institute, Copenhagen, Denmark.

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June 2014

Molecular identification and antifungal susceptibility of yeast isolates causing fungemia collected in a population-based study in Spain in 2010 and 2011.

Antimicrob Agents Chemother 2014 23;58(3):1529-37. Epub 2013 Dec 23.

Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Universidad Complutense de Madrid, Madrid, Spain.

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October 2014

Paradoxical growth of Candida albicans in the presence of caspofungin is associated with multiple cell wall rearrangements and decreased virulence.

Antimicrob Agents Chemother 2014 2;58(2):1071-83. Epub 2013 Dec 2.

Mycology Reference Laboratory, National Center for Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.

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September 2014

Determination of isavuconazole susceptibility of Aspergillus and Candida species by the EUCAST method.

Antimicrob Agents Chemother 2013 Nov 19;57(11):5426-31. Epub 2013 Aug 19.

Department of Molecular and Clinical Pharmacology, Translational Medicine, University of Liverpool, Liverpool, United Kingdom.

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November 2013

Determination of voriconazole serum concentration by bioassay, a valid method for therapeutic drug monitoring for clinical laboratories.

Antimicrob Agents Chemother 2013 Jul 6;57(7):3437-40. Epub 2013 May 6.

Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.

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July 2013

A simple, sensitive HPLC-PDA method for the quantification of itraconazole and hydroxy itraconazole in human serum: a reference laboratory experience.

Diagn Microbiol Infect Dis 2013 Jul 12;76(3):314-20. Epub 2013 Apr 12.

Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain.

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July 2013

Comparison of PCR protocols for detecting Histoplasma capsulatum DNA through a multicenter study.

Rev Iberoam Micol 2013 Oct-Dec;30(4):256-60. Epub 2013 Apr 11.

Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra Majadahonda-Pozuelo, Km 2, 28220 Majadahonda (Madrid), Spain. Electronic address:

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August 2014

[Antifungal susceptibility testing in yeasts. Update and novelties].

Enferm Infecc Microbiol Clin 2013 Feb;31 Suppl 1:53-8

Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, España.

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February 2013

Analysis of strain relatedness using high resolution melting in a case of recurrent candiduria.

BMC Microbiol 2013 Jan 23;13:13. Epub 2013 Jan 23.

Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra Majadahonda-Pozuelo, Km 2, Majadahonda, Madrid, 28220, Spain.

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January 2013

Current section and species complex concepts in Aspergillus: recommendations for routine daily practice.

Ann N Y Acad Sci 2012 Dec;1273:18-24

Mycology Department, Spanish National Center for Microbiology, Instituto de Salud Carlos III, Madrid, Spain.

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December 2012

Breakthrough pulmonary Aspergillus fumigatus infection with multiple triazole resistance in a Spanish patient with chronic myeloid leukemia.

Rev Iberoam Micol 2013 Jan 15;30(1):64-8. Epub 2012 Sep 15.

Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Spain.

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January 2013

Development and validation of a fast HPLC/photodiode array detection method for the measurement of voriconazole in human serum samples. A reference laboratory experience.

Enferm Infecc Microbiol Clin 2013 Jan 8;31(1):23-8. Epub 2012 Jul 8.

Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Spain.

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January 2013

Pharmacodynamics of voriconazole in a dynamic in vitro model of invasive pulmonary aspergillosis: implications for in vitro susceptibility breakpoints.

J Infect Dis 2012 Aug 25;206(3):442-52. Epub 2012 May 25.

Manchester Academic Health Science Centre, National Institute for Health Research Translational Research Facility in Respiratory Medicine, University of Manchester, University Hospital of South Manchester National Health Service Foundation Trust, United Kingdom.

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August 2012

Resistance to voriconazole due to a G448S substitution in Aspergillus fumigatus in a patient with cerebral aspergillosis.

J Clin Microbiol 2012 Jul 9;50(7):2531-4. Epub 2012 May 9.

Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Universidad Complutense, Madrid, Spain.

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July 2012

Fungal infection in solid organ recipients.

Enferm Infecc Microbiol Clin 2012 Mar;30 Suppl 2:49-56

Department of Infectious Diseases, Hospital Ramón y Cajal, Madrid, Spain.

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March 2012

EUCAST technical note on the EUCAST definitive document EDef 7.2: method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for yeasts EDef 7.2 (EUCAST-AFST).

Clin Microbiol Infect 2012 Jul 8;18(7):E246-7. Epub 2012 May 8.

Unit of Mycology, Department of Microbiological Surveillance and Research, Statens Serum Institute, Copenhagen, Denmark.

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July 2012

[Laboratory diagnosis of fungal infection diseases].

Enferm Infecc Microbiol Clin 2012 May 6;30(5):257-64. Epub 2012 Mar 6.

Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, España.

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May 2012

[Current epidemiology and laboratory diagnosis of endemic mycoses in Spain].

Enferm Infecc Microbiol Clin 2012 Aug 29;30(7):407-13. Epub 2011 Nov 29.

Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, España.

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August 2012

Role of Aspergillus lentulus 14-α sterol demethylase (Cyp51A) in azole drug susceptibility.

Antimicrob Agents Chemother 2011 Dec 26;55(12):5459-68. Epub 2011 Sep 26.

Servicio de Micologia, Centro Nacional de Microbiologia. Instituto de Salud Carlos III, Carretera Majadahonda-Pozuelo Km2, 28220 Madrid, Spain.

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December 2011

Cryptococcus neoformans capsular enlargement and cellular gigantism during Galleria mellonella infection.

PLoS One 2011 7;6(9):e24485. Epub 2011 Sep 7.

Mycology Reference Laboratory, National Centre for Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.

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February 2012

Three-dimensional models of 14α-sterol demethylase (Cyp51A) from Aspergillus lentulus and Aspergillus fumigatus: an insight into differences in voriconazole interaction.

Int J Antimicrob Agents 2011 Nov 25;38(5):426-34. Epub 2011 Aug 25.

Mycology Reference Laboratory, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Carretera Majadahonda-Pozuelo Km 2, 28220 Madrid, Spain.

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November 2011

Analysis of performance of a PCR-based assay to detect DNA of Aspergillus fumigatus in whole blood and serum: a comparative study with clinical samples.

J Clin Microbiol 2011 Oct 17;49(10):3596-9. Epub 2011 Aug 17.

Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra Majadahonda-Pozuelo, Km 2. 28220 Majadahonda (Madrid), Spain.

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October 2011

High-resolution melting analysis for identification of the Cryptococcus neoformans-Cryptococcus gattii complex.

J Clin Microbiol 2011 Oct 10;49(10):3663-6. Epub 2011 Aug 10.

Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra Majadahonda-Pozuelo, Km 2, 28220 Majadahonda (Madrid), Spain.

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October 2011

[Candidemia: impact of epidemiological studies on the treatment and prognosis of a serious infection].

Enferm Infecc Microbiol Clin 2011 May;29(5):325-7

Servicio de Enfermedades Infecciosas, Hospital Universitari Vall d'Hebron, Barcelona, España.

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May 2011

The interaction between Candida krusei and murine macrophages results in multiple outcomes, including intracellular survival and escape from killing.

Infect Immun 2011 Jun 21;79(6):2136-44. Epub 2011 Mar 21.

Mycology Reference Laboratory, National Centre for Microbiology, Instituto de Salud Carlos III, Crta. Majadahonda-Pozuelo, Km2, Majadahonda 28220, Madrid, Spain.

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June 2011

Amphotericin B mediates killing in Cryptococcus neoformans through the induction of a strong oxidative burst.

Microbes Infect 2011 May 21;13(5):457-67. Epub 2011 Feb 21.

Mycology Reference Laboratory, National Centre for Microbiology, Instituto de Salud Carlos III, Crta Majadahonda-Pozuelo Km 2, Majadahonda 28220, Madrid, Spain.

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May 2011

In vitro antifungal susceptibility pattern and ergosterol content in clinical yeast strains.

Rev Iberoam Micol 2011 Apr-Jun;28(2):100-3. Epub 2011 Jan 18.

Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, España.

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November 2011

Process analysis of variables for standardization of antifungal susceptibility testing of nonfermentative yeasts.

Antimicrob Agents Chemother 2011 Apr 18;55(4):1563-70. Epub 2011 Jan 18.

Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Carretera Majadahonda-Pozuelo, km 2, Majadahonda 28220, Madrid, Spain.

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April 2011

Histoplasmosis and paracoccidioidomycosis in a non-endemic area: a review of cases and diagnosis.

J Travel Med 2011 Jan-Feb;18(1):26-33. Epub 2010 Nov 28.

Servicio de Micología, Instituto de Salud Carlos III, Carretera Majadahonda-Pozuelo Km 2, Majadahonda, Madrid, Spain.

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February 2011

Detection and investigation of invasive mould disease.

J Antimicrob Chemother 2011 Jan;66 Suppl 1:i15-24

Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra Majadahonda-Pozuelo Km 2, 28220 Majadahonda, Madrid, Spain.

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January 2011

[Antifungal agents in the treatment of systemic infections: Relevance of mechanism of action, activity profile and resistances].

Rev Esp Quimioter 2010 Dec;23(4):169-76

Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra.Majadahonda-Pozuelo Km. 2. Majadahonda, Madrid, Spain.

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December 2010

Fungal cell gigantism during mammalian infection.

PLoS Pathog 2010 Jun 17;6(6):e1000945. Epub 2010 Jun 17.

Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.

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June 2010

Comparison of caspofungin MICs by means of EUCAST method EDef 7.1 using two different concentrations of glucose.

Antimicrob Agents Chemother 2010 Jul 17;54(7):3056-7. Epub 2010 May 17.

Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo Km 2, 28220 Majadahonda, Spain.

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July 2010

Antifungal susceptibility profile of human-pathogenic species of Lichtheimia.

Antimicrob Agents Chemother 2010 Jul 26;54(7):3058-60. Epub 2010 Apr 26.

Servicio de Micologia, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Ctra Majadahonda Pozuelo km 2, 28220 Majadahonda, Spain.

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July 2010

EUCAST breakpoints for antifungals.

Drug News Perspect 2010 Mar;23(2):93-7

Micology Service, National Microbiology Center, Carlos III Health Institute, Madrid, Spain.

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March 2010

Identification of pathogenic rare yeast species in clinical samples: comparison between phenotypical and molecular methods.

J Clin Microbiol 2010 May 17;48(5):1895-9. Epub 2010 Mar 17.

Ctra Majadahonda-Pozuelo Km 2, Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain.

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May 2010

Multicenter comparison of the ISO standard 20776-1 and the serial 2-fold dilution procedures to dilute hydrophilic and hydrophobic antifungal agents for susceptibility testing.

J Clin Microbiol 2010 May 10;48(5):1918-20. Epub 2010 Mar 10.

Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo Km. 2, 28220 Majadahonda, Madrid, Spain.

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May 2010