Publications by authors named "M Kerry O"

19 Publications

Enhanced Corrosion Resistance and In Vitro Biocompatibility of Mg-Zn Alloys by Carbonate Apatite Coating.

ACS Appl Bio Mater 2021 09 18;4(9):6881-6892. Epub 2021 Aug 18.

Department of Materials Science and Engineering, Tokyo Institute of Technology, Tokyo 152-8550, Japan.

B-type carbonate apatite (CAp) coatings were formed on as-cast and T4-treated Mg-Zn ( = 1, 5, and 7 wt %) alloys containing various sized Zn-rich second phase to improve the corrosion resistance and biocompatibility. The CAp coating grew uniformly on the alloys with a thickness of 1.1-1.3 μm and did not show cracks or pores on 30 μm-sized second-phase particles. The CAp coating retarded corrosion of Mg-Zn substrates for the first 3-5 days in Hanks' solution. Polarization resistance of the CAp-coated alloys was 10-90 and 1-70 times higher than the uncoated and hydroxyapatite (HAp)-coated alloys, respectively. The corrosion rate of CAp-coated alloys was greatly affected by the substrate alloys once the coatings were partly broken. The CAp-coated alloys showed 40-60 and 25-45% lower 14-day average corrosion rates than the uncoated and HAp-coated alloys, respectively, in the immersion test. The CAp coating significantly enhanced the viability of osteoblastic MC3T3-E1 cells on the Mg-Zn alloys for 72 h compared to the uncoated and HAp-coated alloys. The cell densities on CAp-coated alloys were similar for 72 h regardless of substrate alloys. Therefore, the CAp coating can be a superior coating candidate for corrosion-control and biocompatibility improvement for biodegradable Mg alloys.
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http://dx.doi.org/10.1021/acsabm.1c00594DOI Listing
September 2021

A Strategic Approach to Use Upcycled Si Nanomaterials for Stable Operation of Lithium-Ion Batteries.

Nanomaterials (Basel) 2021 Nov 30;11(12). Epub 2021 Nov 30.

Energy Storage Materials Center, Korea Institute of Ceramic Engineering and Technology, Jinju 52851, Korea.

Silicon, as a promising next-generation anode material, has drawn special attention from industries due to its high theoretical capacity (around 3600 mAh g) in comparison with conventional electrodes, e.g., graphite. However, the fast capacity fading resulted by a large volume change hinders the pragmatic use of Si anodes for lithium ion batteries. In this work, we propose an efficient strategy to improve the cyclability of upcycled Si nanomaterials through a simple battery operation protocol. When the utilization degree of Si electrodes was decreased, the electrode deformation was significantly alleviated. This directly led to an excellent electrochemical performance over 100 cycles. In addition, the average charge (delithation) voltage was shifted to a lower voltage, when the utilization degree of electrodes was controlled. These results demonstrated that our strategic approach would be an effective way to enhance the electrochemical performance of Si anodes and improve the cost-effectiveness of scaling-up the decent nanostructured Si material.
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http://dx.doi.org/10.3390/nano11123248DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703682PMC
November 2021

Comparison of Oral Fluid and Urine for Detection of Fentanyl Use Using Liquid Chromatography with Tandem Mass Spectrometry.

J Appl Lab Med 2021 11;6(6):1533-1540

Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA.

Background: We compared oral fluid (OF) and urine (UR) for detection of fentanyl (FEN) use in addiction medicine-psychiatry (AMP) clinics.

Methods: We measured FEN and norfentanyl (NRFEN) in UR with a limit of detection (LOD) of 2.0 µg/L and FEN in OF with an LOD of 0.5 µg/L by LC-MS/MS in 311 paired samples and compared the 2 matrices when higher OF and UR LODs were used.

Results: Urine (UR) detected more FEN use than OF using a LOD of 2.0 µg/L and 0.5 µg/L, respectively. FEN and/or NRFEN were detected in 44 and 59 UR specimens, respectively, and FEN in 46 OF specimens (43 OF+UR+, 3 OF+UR-, 16 OF-UR+, and 249 OF-UR-). In UR there were no instances with FEN positive and NORFEN negative. UR creatinine was <20 mg/dL in the 3 OF+UR- specimen pairs. The median OF/UR analyte concentration ratios in positive sample pairs were 0.23 for OF FEN/UR FEN and 0.02 for OF FEN/UR NRFEN.

Conclusions: We demonstrate that UR detects more FEN use than OF in an AMP setting when UR FEN and UR NORFEN LODs of 2.0 µg/L are used. OF is less sensitive than UR in detecting FEN use, but is still valuable for cases with low UR creatinine and/or suspected adulteration or substitution of UR. The UR vs OF comparison statistics are greatly impacted by even minimal adjustments of the LOD.
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http://dx.doi.org/10.1093/jalm/jfab068DOI Listing
November 2021

Assessment of the Safety of Lactobacillus casei IMV B-7280 Probiotic Strain on a Mouse Model.

Probiotics Antimicrob Proteins 2021 12 20;13(6):1644-1657. Epub 2021 Apr 20.

Zabolotny Institute of microbiology and virology, National Academy of Sciences of Ukraine, 154, Akad. Zabolotny str, Kyiv, 03143, Ukraine.

Probiotics, in particular Lactobacillus (lactic acid bacteria, LAB) strains, are widely used in clinical practice. Despite that these probiotics have GRAS (generally regarded as safe) and qualified presumption of safety (QPS) statuses, the safety of particular strains still needs to be thoroughly studied. The aim of the study was to evaluate the safety of Lact. casei IMV B-7280 strain by investigating toxicity and the effects on gut microbiota in experimental animal model. Male BALB/c mice (7-8 weeks, weight 20-24 g) were treated with amounts of Lact. casei IMV B-7280 strain: 5 × 10, 5 × 10, or 5 × 10 CFU/animal once per day during 7 days, or in the amount of 1 × 10 CFU/animal once per day during 3 days (most of the proposed probiotic doses for humans-from 10 to 10 CFU) and monitored during 14 days. Blood tests and serum biochemistry were conducted; the cecal content from mice of the experimental and control groups were freshly collected and analyzed. At the end of the experiments (15th day), the presence of LAB in the heart, liver, kidney, and mesenteric lymph nodes and peripheral blood was determined; histology of the brain, liver, heart, fragments of the small and large intestine, and mesenteric lymph nodes was conducted. Survival rate of BALB/c mice treated with Lact. casei IMV B-7280 strain in different concentrations in toxicity experiments during 14 days was 100%. We observed no signs of toxicity as changes in gait, lethargy, sleep, somatomotor activity as well as changes in fur, eyes, skin and mucous membranes, tremors, behavior pattern, convulsions, salivation, diarrhea, and local injuries in mice from all experimental groups. After administration of probiotic strain, the number of opportunistic bacteria in cecal contents, such as Staphylococcus spp., Candida spp., Pseudomonas spp., and total aerobic and optionally anaerobic bacteria decreased compared to controls; the population of beneficial bacteria such as lactobacilli increased in cecal contents of these mice. LAB were not detected in the peripheral blood, heart, liver, kidneys, and mesenteric lymph nodes after administration of this strain to intact mice. Lact. casei IMV B-7280 strain is safe at dose up to 10 CFU/animal during 3- and 7-day oral administration to mice and has a positive effect on the gut microbiota composition; it could be potentially considered as safe probiotic for humans.
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http://dx.doi.org/10.1007/s12602-021-09789-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055307PMC
December 2021

The seasonal changes of core bacterial community decide sewage purification in sub-plateau municipal sewage treatment plants.

Bioprocess Biosyst Eng 2020 Sep 24;43(9):1609-1617. Epub 2020 Apr 24.

School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou, 730070, China.

The decline of sewage purification efficiency in winter is a frequent problem in sub-plateau municipal sewage treatment plants (MSTPs). Understanding the links between activated sludge (AS) bacterial community and sewage purification is crucial for exploring the cause of this problem. In this study, Illumina high-throughput sequencing technology was applied to investigate the seasonal changes of AS bacterial community in sub-plateau MSTPs. The sequencing result indicates that the bacterial community OTU number, diversity, and relative abundance in winter are significantly lower than that in summer samples. The discriminant linear effect size analysis (LEfSe) reveals that Proteobacteria and Chloroflexi members were enriched in summer AS, while Actinobacteria and Firmicutes were enriched in winter AS. The results indicate that different core bacterial community assembly was developed in summer and winter, respectively. The changes in bacterial community may be the reasons for the lower sewage purification efficiency in winter. Furthermore, redundancy analysis (RDA) shows that temperature and dissolved oxygen (DO) are the principal factors that drive the seasonal changes in the core bacterial community diversity, richness and structure in sub-plateau MSTPs. Thus, the sub-plateau AS selects for a unique community assembly pattern and shapes the particular AS ecosystem. These results expand previous understanding and provide insight into the relationship between bacterial community and performance of sub-plateau MSTPs.
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http://dx.doi.org/10.1007/s00449-020-02352-2DOI Listing
September 2020
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