Publications by authors named "Linlin Yu"

16 Publications

  • Page 1 of 1

Improving Emotion Regulation Through Real-Time Neurofeedback Training on the Right Dorsolateral Prefrontal Cortex: Evidence From Behavioral and Brain Network Analyses.

Front Hum Neurosci 2021 17;15:620342. Epub 2021 Mar 17.

Key Laboratory of Cognition and Personality of Ministry of Education, Faculty of Psychology, Southwest University, Chongqing, China.

We investigated if emotion regulation can be improved through self-regulation training on non-emotional brain regions, as well as how to change the brain networks implicated in this process. During the training period, the participants were instructed to up-regulate their right dorsolateral prefrontal cortex (rDLPFC) activity according to real-time functional near-infrared spectroscopy (fNIRS) neurofeedback signals, and there was no emotional element. The results showed that the training significantly increased emotion regulation, resting-state functional connectivity (rsFC) within the emotion regulation network (ERN) and frontoparietal network (FPN), and rsFC between the ERN and amygdala; however, training did not influence the rsFC between the FPN and the amygdala. However, self-regulation training on rDLPFC significantly improved emotion regulation and generally increased the rsFCs within the networks; the rsFC between the ERN and amygdala was also selectively increased. The present study also described a safe approach that may improve emotion regulation through self-regulation training on non-emotional brain regions.
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http://dx.doi.org/10.3389/fnhum.2021.620342DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010650PMC
March 2021

Serum kisspeptin levels in polycystic ovary syndrome: A meta-analysis.

J Obstet Gynaecol Res 2021 Mar 25. Epub 2021 Mar 25.

Department of Pharmacology, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, China.

Aim: To clarify the association of serum kisspeptin levels in women with polycystic ovary syndrome (PCOS) by meta-analysis.

Methods: Two English databases and two Chinese databases were searched for the relationship between kisspeptin and PCOS published from 2009. After the studies screening according to specific principles, we used STATA 12.0 for meta-analysis. Standardized mean difference (SMD) and its 95% confidence intervals (95% CIs) were used as the effect size and STATA 12.0 software was performed by this meta-analysis.

Results: Nine articles were included in the end, with a total of 1282 participants (699 patients and 583 controls). Heterogeneity between studies was statistically significant. Therefore, the random effects model was used to combine the effects. Meta-analysis showed statistically significant differences in serum kisspeptin levels between the PCOS patients and controls (SMD = 0.57, 95% CI [0.32, 0.82]), which indicated that there is a strong association between serum kisspeptin levels and PCOS. The source of high heterogeneity between the inclusion studies (I = 73.2%) might be due to the small sample size. The larger variation of kisspeptin concentration might be caused by different diagnosis criteria of PCOS and short half-time period of kisspeptin combined with nonstandard testing process.

Conclusion: Serum kisspeptin levels in PCOS patients were higher than non-PCOS patients. It is a hint to indicate us that kisspeptin might be an independent biomarker of PCOS patients.
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http://dx.doi.org/10.1111/jog.14767DOI Listing
March 2021

Qingfei oral liquid inhibited autophagy to alleviate inflammation via mTOR signaling pathway in RSV-infected asthmatic mice.

Biomed Pharmacother 2021 Jun 8;138:111449. Epub 2021 Mar 8.

Department of Traditional Chinese Medicine, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai 200040, China. Electronic address:

Qingfei oral liquid (QF) is a traditional Chinese medicine that has been used to treat patients with viral pneumonia and asthma for decades. Our previous study revealed that QF prevents airway inflammation and reduces airway hyperresponsiveness (AHR) in respiratory syncytial virus (RSV)-infected asthmatic mice. RSV infection can exacerbate asthma in pediatric patients and induce autophagy, which leads to the promotion of inflammatory cytokine production in the pathology of this disease. The effect of QF on regulating autophagy in RSV-infected asthma patients has not been fully elucidated. In this study, we identified compounds of QF by HPLC-DAD-Q-TOF-MS/MS. The RSV infected OVA challenged mice, we evaluated the RSV-infected asthma model. We found that treatment with QF alleviated airway inflammation and mitigated airway AHR in RSV-infected asthmatic mice. In addition, we found that QF inhibited autophagosome formation and the expression of LC3 protein by using electron and laser confocal microscopy, respectively, to assess RSV-infected asthmatic mice lung tissues. Furthermore, QF was found to reduce the quantity of autophagy and its related proteins LC3B (light chain 3B), Beclin-1, p62 and Atg5 (autophagy-related gene 5) and downstream inflammatory cytokines TNF-α, IL-4, IL-6, and IL-13 via an action in mTOR-dependent signaling in vivo and in vitro. These findings suggest that QF can alleviate the inflammation caused by RSV infection in asthmatic mice, and its mechanism may be involved in the regulation of autophagy via the mTOR signaling pathway.
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http://dx.doi.org/10.1016/j.biopha.2021.111449DOI Listing
June 2021

High throughput detection of antibiotic residues in milk by time-resolved fluorescence immunochromatography based on QR code.

Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2020 Sep 27;37(9):1481-1490. Epub 2020 Jul 27.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety , Beijing, People's Republic of China.

Herein, we have successfully established a novel, rapid, and simple lateral-flow immunoassay based on time-resolved fluorescence and biotin-streptavidin to detect the residues of various antibiotics in milk. The fluorescence signal and sensitivity of immunochromatography were enhanced through biotinylated antibody coupled with streptavidin europium microspheres. Moreover, due to the use of a QR Code and fluorescent reader, quantitative detection and real-time data uploading can be achieved. Under the optimal conditions, the various antibiotic residues were detected in the milk samples. The results showed that the limits of detection of tylosin, lincomycin and doxycycline were 0.10, 0.06, and 0.27 ng/mL, respectively. The recoveries of the spiked milk samples were 88.9%~127%, with coefficients of variation less than 11%, and the test strip can be stored at room temperature for 12 months. This study shows that the proposed time-resolved fluorescence immunoassay is sensitive, rapid and reliable, and has the potential to be used for detection of veterinary antibiotic residues in food safety fields.
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http://dx.doi.org/10.1080/19440049.2020.1778192DOI Listing
September 2020

The Effects of Family Financial Stress and Primary Caregivers' Levels of Acculturation on Children's Emotional and Behavioral Problems among Humanitarian Refugees in Australia.

Int J Environ Res Public Health 2020 04 15;17(8). Epub 2020 Apr 15.

Department of Medical Statistics, School of Public Health, Sun Yat-sen University, Zhongshan Road 2, Guangzhou 510080, China.

The present study evaluated the application of the basic and extended (incorporated primary caregivers' levels of acculturation) Family Stress Model (FSM) to understand the effect of family financial stress and primary caregivers' levels of acculturation on children's emotional and behavioral problems among refugees in Australia. A total of 658 refugee children aged 5-17 and their primary caregivers ( = 410) from the third wave of a nationwide longitudinal project were included in this study. We used multilevel structural equation models with bootstrapping to test the indirect effects of family financial stress and caregivers' levels of acculturation (including English proficiency, self-sufficiency, social interaction, and self-identity) on children's emotional and behavioral problems through caregivers' psychological distress and parenting styles. The results showed that the extended FSM improved the model fit statistics, explaining 45.8% variation in children's emotional and behavioral problems. Family financial stress, caregivers' English proficiency, and self-identity had indirect effects on children's emotional and behavioral problems through caregivers' psychological distress and hostile parenting. The findings showed that interventions aimed at reducing caregivers' psychological distress and negative parenting could be effective in alleviating the adverse effects of family financial stress and caregivers' low levels of acculturation on refugee children's mental health.
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http://dx.doi.org/10.3390/ijerph17082716DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215682PMC
April 2020

Intrinsic kinetic model of photoautotrophic microalgae based on chlorophyll fluorescence analysis.

Math Biosci 2019 09 19;315:108234. Epub 2019 Jul 19.

Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang 330022, Jiangxi, China; Key Laboratory of Bioprocess of Jiangxi Province, Jiangxi Science & Technology Normal University, Nanchang 330013, Jiangxi, China. Electronic address:

As photoautotrophic microorganisms, microalgae feature complex mechanisms of photosynthesis and light energy transfer and as such studying their intrinsic growth kinetics is fairly difficult. In this article, the quantum yield of photochemical reaction was introduced in a study of microalgal kinetics to establish an intrinsic kinetic model of photoautotrophic microalgal growth. The blue-green algae Synechococcus sp. PCC7942 was used to verify the kinetic model developed using chlorophyll fluorescence analysis and growth kinetics determination. Results indicate that the kinetic model can realistically reflect the light energy utilization efficiency of microalgae as well as their intrinsic growth kinetic characteristics. The model and method proposed in this article may be utilized in intrinsic kinetics studies of photoautotrophic microorganisms.
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http://dx.doi.org/10.1016/j.mbs.2019.108234DOI Listing
September 2019

Distinct regulation of atonal in a visual organ of Drosophila: Organ-specific enhancer and lack of autoregulation in the larval eye.

Dev Biol 2017 Jan 29;421(1):67-76. Epub 2016 Sep 29.

Department of Ophthalmology and Center for Vision Research, SUNY Upstate Medical University, Syracuse, NY, USA; Departments of Neuroscience & Physiology and Biochemistry & Molecular Biology, SUNY Upstate Medical University, Syracuse, NY, USA. Electronic address:

Drosophila has three types of visual organs, the larval eyes or Bolwig's organs (BO), the ocelli (OC) and the compound eyes (CE). In all, the bHLH protein Atonal (Ato) functions as the proneural factor for photoreceptors and effects the transition from progenitor cells to differentiating neurons. In this work, we investigate the regulation of ato expression in the BO primordium (BOP). Surprisingly, we find that ato transcription in the BOP is entirely independent of the shared regulatory DNA for the developing CE and OC. The core enhancer for BOP expression, ato, lies ~6kb upstream of the ato gene, in contrast to the downstream location of CE and OC regulatory elements. Moreover, maintenance of ato expression in the neuronal precursors through autoregulation-a common and ancient feature of ato expression that is well-documented in eyes, ocelli and chordotonal organs-does not occur in the BO. We also show that the ato enhancer contains two binding sites for the transcription factor Sine oculis (So), a core component of the progenitor specification network in all three visual organs. These binding sites function in vivo and are specifically bound by So in vitro. Taken together, our findings reveal that the control of ato transcription in the evolutionarily derived BO has diverged considerably from ato regulation in the more ancestral compound eyes and ocelli, to the extent of acquiring what appears to be a distinct and evolutionarily novel cis-regulatory module.
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http://dx.doi.org/10.1016/j.ydbio.2016.09.023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5358805PMC
January 2017

ato-Gal4 fly lines for gene function analysis: Eya is required in late progenitors for eye morphogenesis.

Genesis 2015 Jun 10;53(6):347-55. Epub 2015 Jun 10.

Department of Ophthalmology, Center for Vision Research, and SUNY Eye Institute, SUNY Upstate Medical University, Syracuse, New York.

The Gal4/UAS system is one of the most powerful tools for the study of cellular and developmental processes in Drosophila. Gal4 drivers can be used to induce targeted expression of dominant-negative and dominant-active proteins, histological markers, activity sensors, gene-specific dsRNAs, modulators of cell survival or proliferation, and other reagents. Here, we describe novel atonal-Gal4 lines that contain regions of the regulatory DNA of atonal, the proneural gene for photoreceptors, stretch receptors, auditory organ, and some olfactory sensilla. During neurogenesis, the atonal gene is expressed at a critical juncture, a time of transition from progenitor cell to developing neuron. Thus, these lines are particularly well suited for the study of the transcription factors and signaling molecules orchestrating this critical transition. To demonstrate their usefulness, we focus on two visual organs, the eye and the Bolwig. We demonstrate the induction of predicted eye phenotypes when expressing the dominant-negative EGF receptor or a dsRNA against Notch in the developing eye disc. In another example, we show the deletion of the Bolwig's organ using the proapoptotic factor Hid. Finally, we investigate the function of the eye specification factor Eyes absent or Eya in late retinal progenitors, shortly before they begin morphogenesis. We show that Eya is still required in these late progenitors to promote eye formation, and show failure to induce the target gene atonal and consequent lack of neuron formation.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5063080PMC
http://dx.doi.org/10.1002/dvg.22858DOI Listing
June 2015

New pathogenetic characters of reticuloendotheliosis virus isolated from Chinese partridge in specific-pathogen-free chickens.

Microb Pathog 2012 Aug 3;53(2):57-63. Epub 2012 May 3.

Department of Fundamental Veterinary, College of Veterinary Medicine, Shandong Agricultural University, Daizong ST, Tai'an, China.

Avian reticuloendotheliosis virus (REV) infection can induce a runting syndrome, immunosuppression, acute reticulum cell neoplasia and lymphomas in a variety of domestic and wild birds. To evaluate the pathogenicity and oncogenicity of REV-JX0927 that isolated from Chinese partridge, experimental inoculated day-old specific-pathogen-free (SPF) White Leghorn chickens were examined at regular intervals. The examination procedures included hematology, serology and histopathology; also including immunohistochemistry and apoptosis assay. Body weight, relative immune organs weight and apoptosis assay results revealed that the immunosuppression of infected birds is associated with apoptosis of lymphocytes in lymphoid tissues, especially in thymus induced by REV-JX0927. Hematology and apoptosis assay results showed that the 7th week of post-infection is a critical time point for lymphocytes to be transformed into tumor cells. Histopathology evidences demonstrated that REV-JX0927 induced reticuloendotheliosis at early stage (1 week), and lymphosarcomas at middle stage (after 7 weeks). In addition, squamous-cell carcinoma, adenocarcinoma and aneurysm were found in infected birds. Arteritis was associated with concentration of serum protein and fat. REV antigen expression was observed in infected birds through the experimental period. REV has high tropism for proventriculus, kidney, liver, lymphoid tissues, pancreas, lymphosarcoma cells and blood vessels. Data from this study showed that several new pathogenitic characters caused by REV-JX0927 were observed. It indicated that REV-JX0927 is a multipotential oncogenic retrovirus.
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http://dx.doi.org/10.1016/j.micpath.2012.04.001DOI Listing
August 2012

Identification of Bombyx atonal and functional comparison with the Drosophila atonal proneural factor in the developing fly eye.

Genesis 2012 May 27;50(5):393-403. Epub 2011 Dec 27.

Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Science, Zhejiang University, Hangzhou 310029, China.

The proneural genes are fundamental regulators of neuronal development in all metazoans. A critical role of the fly proneural factor Atonal (Ato(Dm)) is to induce photoreceptor neuron formation in Drosophila, whereas its murine homolog, Atonal7(Mm) (aka Ath5) is essential for the development of the ganglion cells of the vertebrate eye. Here, we identify the Bombyx mori ato homolog (ato(Bm) ). In a pattern strikingly reminiscent of ato(Dm), the ato(Bm) mRNA is expressed as a stripe in the silkworm eye disc. Its DNA-binding and protein-protein interaction domain is highly homologous to the Ato(Dm) bHLH. Targeted expression of Ato(Bm) in the endogenous ato(Dm) pattern rescues the eyeless phenotype of the fly ato(1) mutant and its ectopic expression induces similar gain-of-function phenotypes as Ato(Dm). Rescue experiments with chimeric proteins show that the non-bHLH portion of Ato(Bm) (N-region) can effectively substitute for the corresponding region of the fly transcription factor, even though no apparent conservation can be found at the amino acid level. On the contrary, the highly similar bHLH domain of Ato(Bm) cannot similarly substitute for the corresponding region of Ato(Dm). Thus, the bHLH(Bm) domain requires the Ato(Bm) N-region to function effectively, whereas the bHLH(Dm) domain can operate well with either N-region. These findings suggest a role for the non-bHLH portion of Ato proteins in modulating the function of the bHLH domain in eye neurogenesis and implicate specific aa residues of the bHLH in this process.
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http://dx.doi.org/10.1002/dvg.20816DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3362925PMC
May 2012

Ninein-like protein is overexpressed in head and neck squamous cell carcinoma and contributes to cancer growth and resistance to apoptosis.

Oncol Rep 2009 Oct;22(4):789-98

Department of Otorhinolaryngology Head and Neck Surgery, Xuanwu Hospital of Capital Medical University, Beijing 100053, P.R. China.

Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer worldwide and survival rates are not improving. Better understanding of the molecular mechanisms of this disease becomes critical to develop more effective treatments. Ninein-like protein (Nlp), a recently identified centrosome-associated protein, is a key regulator in centrosome maturation, which contributes to chromosome segregation and cytokinesis. Recent studies have revealed overexpression of Nlp in several types of human tumors and suggested it was a potential oncogenic protein. To investigate the role of Nlp in the development of HNSCC, expression of Nlp in tumor tissues of 76 HNSCC patients were analyzed, and the correlations of Nlp expression with the clinicopathological characteristics were evaluated. Our data showed overexpression of Nlp in tumor tissues compared with their normal counterparts. Moreover, overexpression of Nlp correlated with tumor differentiation and immunohistochemistry analysis of preinvasive dysplasia and squamous cell carcinoma showed that overexpression of Nlp occurred in premalignant lesions. Biological studies with human HNSCC cell lines indicated that overexpression of Nlp promoted cell proliferation and inhibited cisplatin-induced apoptosis. Taken together, these results suggest a novel mechanism that is closely related to malignant phenotype and anti-cancer drugs resistance of HNSCC and support the notion that Nlp overexpression might contribute to the development of HNSCC.
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http://dx.doi.org/10.3892/or_00000501DOI Listing
October 2009

Homology of dipteran bristles and lepidopteran scales: requirement for the Bombyx mori achaete-scute homologue ASH2.

Genetics 2009 Oct 10;183(2):619-27, 1SI-3SI. Epub 2009 Aug 10.

The Biotechnology Research Institute, National Engineering of Crop Germplasm and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, China.

Lepidopteran wing scales and Drosophila bristles are considered homologous structures on the basis of the similarities in their cell lineages. However, the molecular mechanisms underlying scale development are essentially unknown as analysis of gene function in Lepidoptera is sorely limited. In this study, we used the Bombyx mori mutant scaleless (sl), which displays a nearly complete loss of wing scales, to explore the mechanism of lepidopteran wing-scale formation. We found that Bm-ASH2, one of four Bombyx achaete-scute homologs, is highly expressed in early pupal wings of wild-type silkworms, but its expression is severely reduced in sl pupal wings. Through molecular characterization of the mutant locus using luciferase and gel shift assays, genetic analysis of recombining populations, and in vivo rescue experiments, we provide evidence that a 26-bp deletion within the Bm-ASH2 promoter is closely linked to the sl locus and leads to loss of Bm-ASH2 expression and the scaleless-wings phenotype. Thus, the Bm-ASH2 appears to play a critical role in scale formation in B. mori. This finding supports the proposed homology of lepidopteran scales and dipteran bristles and provides evidence for conservation of the genetic pathway in scale/bristle development at the level of gene function.
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http://dx.doi.org/10.1534/genetics.109.102848DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2766321PMC
October 2009

Prognostic value of Tiam1 and Rac1 overexpression in nasopharyngeal carcinoma.

ORL J Otorhinolaryngol Relat Spec 2009 9;71(3):163-71. Epub 2009 Jun 9.

Department of Otorhinolaryngology Head and Neck Surgery, XuanWu Hospital, Capital Medical University, Beijing 100053, China.

Purpose: This study is designed to investigate the roles of Tiam1 and Rac1 in nasopharyngeal carcinoma (NPC).

Methods: NPC samples (n = 102) were analyzed with immunohistochemistry for Tiam1 and Rac1 proteins, 28 of which were also analyzed with quantitative RT-PCR and Western blots for Tiam1 and Rac1 mRNA and protein expression. The expression of Tiam1 and Rac1 in noncancerous nasopharyngeal tissue (n = 26) acted as a control. The expression was gauged regarding stage, grade and survival.

Results: Tiam1 and Rac1 were overexpressed in NPC cells and their protein upregulation was significantly associated with stage and grading (p < 0.05 for all). Cox regression analysis revealed Tiam1 and Rac1 protein upregulation was correlated with lower disease-free and overall survival rates (p < 0.001).

Conclusions: Upregulation of Tiam1 and Rac1 proteins may play a critical role in tumor progression of NPC, and work as a prognostic factor for NPC patients.
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http://dx.doi.org/10.1159/000223440DOI Listing
October 2009

Analysis of four achaete-scute homologs in Bombyx mori reveals new viewpoints of the evolution and functions of this gene family.

BMC Genet 2008 Mar 6;9:24. Epub 2008 Mar 6.

The Biotechnology Research Institute, National Engineering of crop germplasm and genetic improvement, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Background: achaete-scute complexe (AS-C) has been widely studied at genetic, developmental and evolutional levels. Genes of this family encode proteins containing a highly conserved bHLH domain, which take part in the regulation of the development of central nervous system and peripheral nervous system. Many AS-C homologs have been isolated from various vertebrates and invertebrates. Also, AS-C genes are duplicated during the evolution of Diptera. Functions besides neural development controlling have also been found in Drosophila AS-C genes.

Results: We cloned four achaete-scute homologs (ASH) from the lepidopteran model organism Bombyx mori, including three proneural genes and one neural precursor gene. Proteins encoded by them contained the characteristic bHLH domain and the three proneural ones were also found to have the C-terminal conserved motif. These genes regulated promoter activity through the Class A E-boxes in vitro. Though both Bm-ASH and Drosophila AS-C have four members, they are not in one by one corresponding relationships. Results of RT-PCR and real-time PCR showed that Bm-ASH genes were expressed in different larval tissues, and had well-regulated expressional profiles during the development of embryo and wing/wing disc.

Conclusion: There are four achaete-scute homologs in Bombyx mori, the second insect having four AS-C genes so far, and these genes have multiple functions in silkworm life cycle. AS-C gene duplication in insects occurs after or parallel to, but not before the taxonomic order formation during evolution.
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http://dx.doi.org/10.1186/1471-2156-9-24DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2315653PMC
March 2008

[Expression of hypoxia-inducible factor-1alpha in middle ear cholesteatoma].

Lin Chuang Er Bi Yan Hou Ke Za Zhi 2005 Jan;19(1):4-6

Department of Otolaryngology, Tongji Hospital, Wuhan, 430030, China.

Objective: To explore the pathogenetic mechanism of middle ear cholesteatoma, the aim of this study is to detect the expression of Hypoxia-inducible factor-1alpha in middle ear cholesteatoma and normal external ear canal skin.

Method: We used the technology of immunohistochemistry to examine the expression of Hypoxia-inducible factor-1alpha in thirty-one middle ear cholesteatomas and ten samples of normal external ear canal skin.

Result: The expression of HIF-1alpha was extremely higher in middle ear cholesteatomas than in normal external ear canal skin (P <0.05).

Conclusion: We found that the higher expression of Hypoxia-inducible factor-1alpha in middle ear cholesteatomas, so we think that Hypoxia-inducible factor-1alpha play an important role in the pathogenetic process of middle ear cholesteatoma, and hypoxia may be an incentive in the pathogenetic mechanism of middle ear cholesteatoma.
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January 2005

Expression of hypoxia inducible factor-1alpha and its relationship to apoptosis and proliferation in human laryngeal squamous cell carcinoma.

J Huazhong Univ Sci Technolog Med Sci 2004 ;24(6):636-8

Department of Otolaryngology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

To investigate the expression of hypoxia inducible factor-1 alpha (HIF-1alpha) and its relationship to apoptosis and proliferation in laryngeal squamous cell carcinoma (LSCC), immunohistochemical method was used to detect the expression of HIF-1alpha and PCNA. Tunnel technique was used to detect in situ cell apoptosis in LSCC. Our results showed that the expression of HIF-1alpha was related to the clinical stages of cancer and lymph node metastasis (P<0.05). The relationship between HIF-1alpha and PCNA was statistically significant (P<0.05) and no relationship was found between HIF-1alpha and apoptosis (P>0.05) It is concluded that HIF-1alpha plays a role in the carcinogenesis of laryngeal carcinoma and is correlated with proliferation, but bears no relationship with the apoptosis of tumor cells in LSCC.
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http://dx.doi.org/10.1007/BF02911379DOI Listing
May 2006