Publications by authors named "Lei Zheng"

1,142 Publications

  • Page 1 of 1

Effects of Environmental Worry on Fruit and Vegetable Intake.

Int J Behav Med 2021 Aug 2. Epub 2021 Aug 2.

School of Psychological and Cognitive Sciences and Beijing Key Laboratory of Behavior and Mental Health, Peking University, Beijing, China.

Background: Environmental worry, a response to environmental pollution, is a well-established predictor of pro-environmental behaviors. However, its relationship with health-promoting behaviors, such as fruit and vegetable intake (FVI), is unclear. In three studies, we explored the associations between environmental worry, behavioral intention, and FVI.

Method: Study 1, enrolling 358 company trainees who provided their data at Time 1 (T1) and Time 2 (T2; a 2-month follow-up), examined the relationship between environmental worry and FVI. Study 2, enrolling 226 employees providing their data at T1 and T2 (a 2-month follow-up), examined the mediating effects of intention in the relationship between environmental worry and FVI using a cross-lagged design. In study 3, data from 217 participants were collected three times over a period of 4 months. We re-examined the mediating effect of intention and further explored the moderating effect of future focus.

Results: Environmental worry predicted beneficial changes in FVI (studies 1, 2, and 3). Environmental worry predicted FVI through intention (studies 2 and 3). The mediating effect of intention on the relationship between environmental worry and FVI was moderated by future focus: individuals with high environmental worry formed stronger intention when they also reported a strong future focus (study 3).

Conclusions: Our findings provide novel evidence for the direct and indirect links between environmental worry and health-promoting behavior (i.e., FVI).
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http://dx.doi.org/10.1007/s12529-021-10012-2DOI Listing
August 2021

Diffusion tensor tractography of heatstroke.

Acta Neurol Belg 2021 Aug 2. Epub 2021 Aug 2.

Department of Radiology, Yantai Affiliated Hospital of Binzhou Medical Universtiy, Yantai, China.

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http://dx.doi.org/10.1007/s13760-021-01755-6DOI Listing
August 2021

A fluorescent screening method for optimization of conotoxin expression in Pichia pastoris.

Biotechnol Appl Biochem 2021 Aug 1. Epub 2021 Aug 1.

Fujian Provincial Key laboratory of Plant Functional Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Conotoxins are small cysteine-rich peptides secreted by the Conus venom glands, which act on ion channels or membrane receptors with high specificity and potency. Conotoxins are invaluable sources for neuroscience research and drug leads, but their application is hindered by the limited successes in quantitative engineering using either chemical or biotechnological approaches. Here, we explore the Pichia pastoris to express 23 selected conopeptides using a GFP-based fluorescence screen. We found that, in a protease-deficient strain PichiaPink™ Strain 4 (ade2 prb1 pep4), most of the recombinant conopeptides were expressed as two major folding variants including a compact form that was somehow resistant to reduction and high temperature. The GFP-αTxIA was the only one displaying a single band that showed a dose-dependent neurotoxicity on larvae of the insect Plutella xylostella, with a 48-hour LD lower than 1.12 pmol mg body weight. Furthermore, the recombinant αTxIA after cleavage from the fusion was able to inhibit cell proliferation of the LYCT and HEK293T cell lines with an appearance IC of 341 ± 8 and 235 ± 15 nM, respectively. This screening method is straightforward and easy to scale-up, providing a versatile tool for further optimization of conotoxin production in the yeast cell. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/bab.2231DOI Listing
August 2021

Identification of Disease-Related 2-Oxoglutarate/Fe (II)-Dependent Oxygenase Based on Reduced Amino Acid Cluster Strategy.

Front Cell Dev Biol 2021 16;9:707938. Epub 2021 Jul 16.

State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Sciences, Inner Mongolia University, Hohhot, China.

The 2-oxoglutarate/Fe (II)-dependent (2OG) oxygenase superfamily is mainly responsible for protein modification, nucleic acid repair and/or modification, and fatty acid metabolism and plays important roles in cancer, cardiovascular disease, and other diseases. They are likely to become new targets for the treatment of cancer and other diseases, so the accurate identification of 2OG oxygenases is of great significance. Many computational methods have been proposed to predict functional proteins to compensate for the time-consuming and expensive experimental identification. However, machine learning has not been applied to the study of 2OG oxygenases. In this study, we developed OGFE_RAAC, a prediction model to identify whether a protein is a 2OG oxygenase. To improve the performance of OGFE_RAAC, 673 amino acid reduction alphabets were used to determine the optimal feature representation scheme by recoding the protein sequence. The 10-fold cross-validation test showed that the accuracy of the model in identifying 2OG oxygenases is 91.04%. Besides, the independent dataset results also proved that the model has excellent generalization and robustness. It is expected to become an effective tool for the identification of 2OG oxygenases. With further research, we have also found that the function of 2OG oxygenases may be related to their polarity and hydrophobicity, which will help the follow-up study on the catalytic mechanism of 2OG oxygenases and the way they interact with the substrate. Based on the model we built, a user-friendly web server was established and can be friendly accessed at http://bioinfor.imu.edu.cn/ogferaac.
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http://dx.doi.org/10.3389/fcell.2021.707938DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8323781PMC
July 2021

Novel strategies for immuno-oncology breakthroughs with cell therapy.

Biomark Res 2021 Jul 31;9(1):62. Epub 2021 Jul 31.

Chinese American Hematologist and Oncologist Network, New York, NY, USA.

Cell therapy has evolved rapidly in the past several years with more than 250 clinical trials ongoing around the world. While more indications of cellular therapy with chimeric antigen receptor - engineered T cells (CAR-T) are approved for hematologic malignancies, new concepts and strategies of cellular therapy for solid tumors are emerging and are discussed. These developments include better selections of targets by shifting from tumor-associated antigens to personalized tumor-specific neoantigens, an enhancement of T cell trafficking by breaking the stromal barriers, and a rejuvenation of exhausted T cells by targeting immunosuppressive mechanisms in the tumor microenvironment (TME). Despite significant remaining challenges, we believe that cell therapy will once again lead and revolutionize cancer immunotherapy before long because of the maturation of technologies in T cell engineering, target selection and T cell delivery. This review highlighted the recent progresses reported at the 2020 China Immuno-Oncology Workshop co-organized by the Chinese American Hematologist and Oncologist Network (CAHON), the China National Medical Product Administration (NMPA), and Tsinghua University.
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http://dx.doi.org/10.1186/s40364-021-00316-6DOI Listing
July 2021

[Screening for UV-C irradiation-enhanced transcription factors that regulate the metabolism of phenolic compounds in tomato fruit].

Sheng Wu Gong Cheng Xue Bao 2021 Jul;37(7):2483-2494

School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, Anhui, China.

Solanum lycopersicum phenylalanine ammonia-lyase 5 (SlPAL5) gene regulates the metabolism of phenolic compounds. The study of transcription factors that regulate the expression of SlPAL5 gene is of great significance to elucidate the regulatory mechanism underlying the biosynthesis of phenolic compounds in tomato fruit induced by UV-C irradiation. Here, yeast one-hybrid library of tomato fruit was constructed, and the yeast one-hybrid technology was used to screen the transcription factors that regulate the expression of SlPAL5, the key gene related to the synthesis of phenolic compounds in tomato fruit. As a result, a transcription factor, SlERF7, was obtained and sequenced, followed by the blast homology analysis. Further experiments confirmed that SlERF7 interacted with the promoter of SlPAL5 gene. In addition, UV-C irradiation significantly increased the expression level of SlERF7. These results indicate that SlERF7, which is regulated by UV-C irradiation, might be involved in regulating the transcription of SlPAL5, which provided foundations for further studying the regulation mechanism of the biosynthesis of phenolic compounds in tomato fruit induced by UV-C irradiation.
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http://dx.doi.org/10.13345/j.cjb.200515DOI Listing
July 2021

Dithienocoronene diimide (DTCDI)-derived triads for high-performance air-stable, solution-processed balanced ambipolar organic field-effect transistors.

Phys Chem Chem Phys 2021 Aug;23(30):16357-16365

Key Laboratory of Applied Surface and Colloid Chemistry, National Ministry of Education, Shaanxi Key Laboratory for Advanced Energy Devices, Shaanxi Engineering Lab for Advanced Energy Technology, School of Materials Science and Engineering, Shaanxi Normal University, Xian 710119, China.

Developing ambipolar organic semiconducting materials is essential for use in complementary-like inverters and light-emitting transistors. In this study, three new dithienocoronenediimide (DTCDI)-derived triads, DTCDI-BT, DTCDI-BBT and DTCDI-BNT, were designed and synthesized, in which various sizes of terminal groups, i.e., thiophene (T), benzo[b]thiophene (BT) and naphtha[2,3-b]thiophene (NT) were substituted at the α-positions of the two thiophene rings of DTCDI, respectively. The DFT calculations reveal that the HOMO energy levels of the three triads when compared to that of the parent DTCDI-core (-5.99 eV) are significantly increased to -5.59, -5.59 and -5.45 eV for DTCDI-BT, DTCDI-BBT and DTCDI-BNT, respectively, whereas the LUMO energy levels (-3.07 eV ∼ -3.14 eV) are almost identical with that of the DTCDI-core (-3.10 eV). The results predict that the triads could possess ambipolar transport properties in organic field-effect transistor (OFET) applications. In fact, under an ambient atmosphere, solution-processed bottom-gate top-contact (BGTC) transistors exhibit ambipolar charge transport properties by tuning the HOMOs of the DTCDI-based triads so that they were suitable for hole injection, resulting in balanced maximum electron and hole mobilities of 1.66 × 10-3 and 1.02 × 10-3 cm2 V-1 s-1 for DTCDI-BT, 2.60 × 10-2 and 3.60 × 10-2 cm2 V-1 s-1 for DTCDI-BBT, and 2.43 × 10-3 and 4.15 × 10-3 cm2 V-1 s-1 for DTCDI-BNT, respectively. This is the first time that the DTCDI building block has been used to develop ambipolar small molecular semiconductors, and achieved a device performance comparable to that of the DTCDI-based polymeric semiconductors. In addition, DTCDI-BBT-based complementary-like inverters were made, and the inverter devices operated well in both p-mode and n-mode under ambient conditions. The results show that the DTCDI is a promising π-electron-deficient building block which could be further used to develop ambipolar semiconducting materials for OFET devices.
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http://dx.doi.org/10.1039/d1cp02703dDOI Listing
August 2021

Pharmacist-led, prescription intervention system-assisted feedback to reduce prescribing errors: A retrospective study.

J Clin Pharm Ther 2021 Jul 27. Epub 2021 Jul 27.

Department of Intensive Care Unit, Shandong Provincial Third Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, P.R. China.

What Is Known And Objective: Prescribing errors are prevalent in hospital settings, with provision of feedback recommended to support prescribing by doctors. To evaluate the impact of a pharmacist-led prescription intervention system on prescribing error rates and to measure intervention efficiency.

Methods: All prescribers in Shandong Provincial Third Hospital received feedback from ward pharmacists using a pharmacist-led prescription intervention system. The prescribing error rate was calculated from Oct 2019 to December 2020. After the intervention was applied, the rates of PASS 1 (System pass), PASS 2 (Pharmacist pass) and PASS 3 (Pharmacist-doctor pass) events and the feedback time were calculated each month.

Results And Discussion: Irrational use of drugs was reduced and the prescription rate increased significantly. The error rate reduced from 6.94% to 1.96%, representing an estimated 71.76% decrease overall (p < 0.05). The PASS 1 rate gradually increased from 88% to 96% (p < 0.05), the PASS 2 rate gradually decreased from 5.06% to 2.04% (p < 0.05), the PASS 3 rate gradually decreased from 6.94% to 1.96% (p < 0.05).

What Is New And Conclusion: The pharmacist-led prescription intervention system has the potential to reduce prescribing errors and improve prescribing outcomes and patient safety.
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http://dx.doi.org/10.1111/jcpt.13491DOI Listing
July 2021

Simultaneous and accurate screening of multiple genetically modified organism (GMO) components in food on the same test line of SERS-integrated lateral flow strip.

Food Chem 2021 Jul 14;366:130595. Epub 2021 Jul 14.

Engineering Research Center of Bioprocess, MOE, School of Food & Biological Engineering, Hefei University of Technology, Hefei 230009, China; Intelligent Manufacturing Institute of Hefei University of Technology, Hefei 230009, China. Electronic address:

Herein, a surface-enhanced Raman scattering (SERS)-integrated LFS platform was developed for rapid and simultaneous screening of multiple genetically modified organism (GMO) components (promoter, codon, and terminator) in soybean. Research demonstrated that, on the same test line (T line) of single LFS, three different GMP components can be well distinguished with the help of three SERS nano tags. Good linear correlations between SERS signal and concentration of each GMO component were also obtained for quantitative analysis. Of greater importance, whether these multiple analytes coexisted or not, varied in the same concentration trend or not, these multiple GMP components can be rapidly (15 min) and accurately screened with satisfied sensitivity and specificity by decoding the signals on the same T line. We envision that this decoding platform can further improve the potential of LFS and SERS for practical applications and provide a promising alternative for multiple screening of GMO identification in food.
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http://dx.doi.org/10.1016/j.foodchem.2021.130595DOI Listing
July 2021

Evolution trace of SARS-CoV-2 from January 19 to March 12, 2020, in the United States.

J Med Virol 2021 Jul 22. Epub 2021 Jul 22.

College of Life Science, Sichuan Agriculture University, Ya'an, China.

As a kind of human betacoronavirus, SARS-CoV-2 has endangered globally public health. As of January 2021, the virus had resulted in 2,209,195 deaths. By studying the evolution trend and characteristics of 265 SARS-CoV-2 strains in the United States from January to March, it is found that the strains can be divided into six clades, USA clade-1, USA clade-2, USA clade-3, USA clade-4, USA clade-5, and USA clade-6, in which US clade-1 may be the most ancestral clade, USA clade-2 is an interim clade of USA clade-1 and USA clade-3, the other three clades have similar codon usage pattern, while USA clade-6 is the newest and most adaptable clade. Mismatch analysis and protein alignment showed that the evolution of the clades arises from some special mutations in viral proteins, which may help the strain to invade, replicate, transcribe and so on. Compared with previous research and classifications, we suggest that clade O in GISAID should not be an independent clade and Wuhan-Hu-1 (EPI_ISL_402125) should not be an ancestral reference sequence. Our study decoded the evolutionary dynamic of SARS-CoV-2 in the early stage from the United States, which give some clues to infer the current evolution trend of SARS-CoV-2 and study the function of viral mutational protein.
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http://dx.doi.org/10.1002/jmv.27225DOI Listing
July 2021

PLA2G4A promotes right-sided colorectal cancer progression by inducing CD39+γδ Tregs polarization.

JCI Insight 2021 Jul 20. Epub 2021 Jul 20.

Department of Colorectal Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.

γδ T cell is a promising candidate cell in tumor immunotherapy. However, γδ T cells polarized to CD39+γδ Tregs upon colorectal cancer (CRC) induction and the underlying mechanism remains unclear. Here, we discovered that the frequency of CD39+γδ Tregs, which positively correlated with poor prognosis, was significantly higher in right-sided CRC (RSCRC) than in the left-sided CRC (LSCRC). Interestingly, CD39+γδ Tregs from RSCRC showed stronger immunosuppressive phenotype and function than LSCRC. Further, the quantitative mass spectrometry data showed that CD39+γδ Tregs polarization was related to the abnormal activation of the PLA2G4A/AA metabolic pathway in RSCRC. Using an in vitro co-culture system and an orthotopic murine model of CRC, we proved that the overexpression of Pla2g4a in CT26 cells induced CD39+γδ Tregs inhibiting the anti-tumor immune response. Finally, we found that the overall survival of the PLA2G4Ahigh group was significantly shortened compared to PLA2G4Alow RSCRC, while the survival of LSCRC was on the contrary. Collectively, RSCRC with abnormal PLA2G4A expression educates γδ T cells into CD39+γδ Tregs to promote tumor progression and metastasis. Our work highlights the interaction between cancer cells and immune cells by distinguishing the primary tumor site and deepens the understanding of tumor microenvironment and immunosuppression.
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http://dx.doi.org/10.1172/jci.insight.148028DOI Listing
July 2021

Effects of postharvest cysteine treatment on sensory quality and contents of bioactive compounds in goji fruit.

Food Chem 2021 Jul 6;366:130546. Epub 2021 Jul 6.

School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China; Research Laboratory of Agricultural Environment and Food Safety, Anhui Modern Agricultural Industry Technology System, Hefei 230009, China. Electronic address:

Effects of cysteine (Cys) treatments (0, 0.01%, 0.05% and 0.10%) on sensory quality and bioactive compounds in goji fruit stored at 4 °C and 90% RH for 10 d were investigated. Results indicated that 0.05% Cys treatment significantly reduced decay ratio and weight loss, and maintained total soluble solid content in goji fruit. Furthermore, 0.05% Cys treatment increased the contents of total phenolic, ascorbic acid and total glutathione, and the ratio of glutathione/oxidized glutathione, resulting in the higher antioxidant capacity. Determination of five free amino acids showed that 0.05% Cys treatment increased the Pro and Tau contents, while had no significant effect on the Cys, Glu and GABA contents. The increase in Tau content might be due to the up-regulation of two key genes involved in the Tau synthesis including CDO and CSAD. These findings suggested that Cys treatment could improve the storage quality in goji fruit.
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http://dx.doi.org/10.1016/j.foodchem.2021.130546DOI Listing
July 2021

New staging classification for pancreatic neuroendocrine neoplasms combining TNM stage and WHO grade classification [].

Cancer Lett 2021 Jul 14;518:207-213. Epub 2021 Jul 14.

Department of Biliary-Pancreatic Surgery, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, China. Electronic address:

AJCC TNM stage and WHO grade (G) are two widely used staging systems to guide clinical management for pancreatic neuroendocrine neoplasms (panNENs), based on clinical staging and pathological grading information, respectively. We proposed to integrate TNM stage and G grade into one staging system (TNMG) and to evaluate its clinical application as a prognostic indicator for panNENs. Accordingly, 5254 patients diagnosed with panNENs were used to evaluate and to validate the applicability of TNMG to panNENs. The predictive accuracy of TNMG system was compared with that of each separate staging/grading system. We found that TNM stage and G grade were independent risk factors for survival in both the Surveillance, Epidemiology, and End Result (SEER) and multicenter series. The interaction effect between TNM stage and G grade was significant. Twelve subgroups combining the TNM stage and G grade were proposed in the TNMG stage, which were classified into five stages TNMG. According to the TNMG staging classification in the SEER series, the estimated median survival for stages I, II, III, IV, and V were 203, 174, 112, 61, and 8 months, respectively. The predictive accuracy of TNMG stage was higher than that of TNM stage and G grade used independently. The TNMG stage classification was more accurate in predicting panNEN patient's prognosis than either the TNM stage or G grade.
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http://dx.doi.org/10.1016/j.canlet.2021.07.018DOI Listing
July 2021

Prognostic Nomograms for Predicting Overall Survival and Cancer-Specific Survival of Patients With Malignant Pheochromocytoma and Paraganglioma.

Front Endocrinol (Lausanne) 2021 21;12:684668. Epub 2021 Jun 21.

Department of Urology, People's Hospital of Tibet Autonomous Region, Lhasa, China.

Background: Malignant pheochromocytoma and paraganglioma (PPGL) are rare tumors with few prognostic tools. This study aimed to construct nomograms for predicting 3- and 5-year survival for patients with malignant PPGL.

Methods: The patient data was retrieved from the Surveillance, Epidemiology, and End Results (SEER) database. A total of 764 patients diagnosed with malignant PPGL from 1975 to 2016 were included in this study. The patients were randomly divided into two cohorts; the training cohort (n = 536) and the validation cohort (n = 228). Univariate analysis, Lasso regression, and multivariate Cox analysis were used to identify independent prognostic factors, which were then utilized to construct survival nomograms. The nomograms were used to predict 3- and 5-year overall survival (OS) and cancer-specific survival (CSS) for patients with malignant PPGL. The prediction accuracy of the nomogram was assessed using the concordance index (C-index), receiver operating characteristic (ROC) curves and calibration curves. Decision curve analysis (DCAs) was used to evaluate the performance of survival models.

Results: Age, gender, tumor type, tumor stage, or surgery were independent prognostic factors for OS in patients with malignant PPGL, while age, tumor stage, or surgery were independent prognostic factors for CSS ( <.05). Based on these factors, we successfully constructed the OS and CSS nomograms. The C-indexes were 0.747 and 0.742 for the OS and CSS nomograms, respectively. In addition, both the calibration curves and ROC curves for the model exhibited reliable performance.

Conclusion: We successfully constructed nomograms for predicting the OS and CSS of patients with malignant PPGL. The nomograms could inform personalized clinical management of the patients.
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http://dx.doi.org/10.3389/fendo.2021.684668DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257089PMC
June 2021

Detailed pedigree analyses and prenatal diagnosis for a family with mucopolysaccharidosis type II.

BMC Med Genomics 2021 Jun 30;14(1):175. Epub 2021 Jun 30.

National Research Institute for Health and Family Planning, National Human Genetic Resources Center, Graduate School of Peking Union Medical College, Beijing, 100081, China.

Background: Mucopolysaccharidosis type II (MPS II) is an X-linked multisystem disorder caused by mutations in the gene encoding iduronate 2-sulfatase (IDS). The clinical manifestations of MPS II include skeletal deformities, airway obstruction, cardiomyopathy, and neurologic deterioration. MPS II has high genetic heterogeneity disorder, and ~ 658 variants of IDS have been reported.

Methods: We undertook a detailed pedigree analysis of four patients within the same family by targeted next-generation sequencing and Sanger sequencing.

Results: We identified a novel heterozygous frameshift variant, c.1224delC(p.Pro408ProfsTer31), of IDS in three patients. We defined c.1224delC as a pathogenic variant according to the 2015 guidelines set by the American College of Medical Genetics and Genomics.

Conclusion: We reported the second Chinese female MPS II patient. We helped to ensure that these two families had healthy babies. Our findings have enlarged the mutational spectrum of IDS, and these findings could be useful for genetic counseling and the prenatal diagnosis of MPS II.
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http://dx.doi.org/10.1186/s12920-021-01027-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247175PMC
June 2021

Revisit of flap factors relating to partial necrosis of distally based sural flaps: an analysis of 435 cases in a single center.

Eur J Trauma Emerg Surg 2021 Jun 28. Epub 2021 Jun 28.

Department of Orthopedics, Henan Provincial People's Hospital, Zhengzhou, 450003, Henan, People's Republic of China.

Background: Partial necrosis is an ongoing topic in regard to flap complications of the distally based sural (DBS) flap However, the factors influencing partial necrosis of the flap remain in debate. The aim of the present study is to further illuminate the flap-related risk factors and the effects of several technical improvements.

Methods: We retrospectively reviewed the data of 429 patients who underwent 435 DBS flaps between April 2001 and December 2019. The reconstruction outcomes, flap viability-related complications, and potential risk factors were compared between the survival group and partial necrosis group, as well as between group A (the procedures performed from April 2001 to March 2010) and group B (those from April 2010 to December 2019).

Results: Among the 435 flaps, 39 flaps (8.97%) exhibited partial necrosis. With the increase of the top edge of the flap, the partial necrosis rate increased significantly (P < 0.05). When the length-to-width ratio [LWR] of the flap was more than 5:1 or the total length of the flap (the length of the skin island plus the length of the adipofascial pedicle) was more than 20 cm, the partial necrosis rate increased significantly (P < 0.05). The partial necrosis rate in group B (5.86%, 15/256) was significantly lower than that in group A (13.41%, 24/179) (P = 0.007).

Conclusions: The top edge of the flap is an essential indicator for predicting the prognosis of the DBS flap. When the total length of the flap is more than 20 cm or the LWR of the flap more than 5:1, the partial necrosis rate will increase significantly. Various technical modifications can lower the top edge of the flap and reduce the LWR of the flap and width of the skin island, and thus improve the flap survival effectively.
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http://dx.doi.org/10.1007/s00068-021-01737-6DOI Listing
June 2021

Prediction of harmful algal blooms in large water bodies using the combined EFDC and LSTM models.

J Environ Manage 2021 Oct 23;295:113060. Epub 2021 Jun 23.

College of Water Sciences, Beijing Normal University, Beijing, 100875, China.

Harmful algal blooms (HABs) is a worldwide water environmental problem. HABs usually happens in short time and is difficult to be controlled. Early warning of HABs using data-driven models is prospective in making time for taking precaution against HABs. High-frequency water quality monitoring data are necessary to improve the reliability of the model, but it is expensive. This research used environmental fluid dynamics code (EFDC) to extend one-point data obtained by only one instrument to the whole 249 ha water area instead of multi-instruments monitoring, followed by Long short-term memory (LSTM) to predict the HABs in the whole water body. Correlation analysis and principal component analysis were used to reduce the data dimension and improve model accuracy. Finally, the LSTM model was calibrated to predict chlorophyll-a (Chl-a) for the next 1 to 3 time steps. The Nash-Sutcliffe efficiency coefficient (NSE) and mean absolute percentage error (MAPE) of EFDC-LSTM were 0.797-0.991 and 2.74-13.16%, respectively, suggesting the promising utilization of this model in early warning systems for HABs. EFDC-LSTM achieves high-precision HABs forecasting in a cost-effective manner, providing a reliable way to detect HABs in advance.
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http://dx.doi.org/10.1016/j.jenvman.2021.113060DOI Listing
October 2021

Epidemiological and clinical characteristics of respiratory viruses in 4403 pediatric patients from multiple hospitals in Guangdong, China.

BMC Pediatr 2021 06 17;21(1):284. Epub 2021 Jun 17.

Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Background: Acute respiratory infections (ARI) cause considerable morbidity and mortality worldwide, especially in children. Unfortunately, there are limited multi-center data on common viral respiratory infections in south China.

Methods: A total of 4403 nasal swabs were collected from children in 10 cities in Guangdong, China in 2019. Seven respiratory viruses, influenza A virus (IFA), influenza B virus (IFB), respiratory syncytial virus (RSV), adenoviruses (ADV) and parainfluenza virus types 1-3 (PIV1, PIV2 and PIV3), were detected by direct immunofluorescence antibody assay. The personal information and clinical characteristics were recorded and analyzed.

Results: The results showed that at least one virus was detected in 1099 (24.96 %) samples. The detection rates of RSV, IFA, ADV, PIV3, PIV1 and PIV2 were 7.13 % (314/4403), 5.31 % (234/4403), 4.02 % (177/4403), 3.04 % (134/4403), 1.70 % (75/4403) and 1.16 % (51/4403), respectively. The detection rate of RSV was highest in 0-6-month-old children at 18.18 % (106/583), while the detection rate of IFA was highest in 12-18-year-old children at 20.48 % (17/83). The total detection rates in winter and spring were 35.67 % (219/614) and 34.56 % (403/1166), higher than those in summer, 17.41 % (284/1631), and autumn, 19.46 % (193/992).

Conclusions: RSV and IFA were the main respiratory viruses in children. With increasing age the detection rate of RSV decreased in children, but the trends for the detection rates of IFA and IFB were the opposite. This study provided the viral etiology and epidemiology of pediatric patients with ARI in Guangdong, China.
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http://dx.doi.org/10.1186/s12887-021-02759-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212487PMC
June 2021

Stable Lithium-Carbon Composite Enabled by Dual-Salt Additives.

Nanomicro Lett 2021 Apr 17;13(1):111. Epub 2021 Apr 17.

i-Lab, CAS Center for Excellence in Nanoscience, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Science, Suzhou, 215123, People's Republic of China.

Lithium metal is regarded as the ultimate negative electrode material for secondary batteries due to its high energy density. However, it suffers from poor cycling stability because of its high reactivity with liquid electrolytes. Therefore, continuous efforts have been put into improving the cycling Coulombic efficiency (CE) to extend the lifespan of the lithium metal negative electrode. Herein, we report that using dual-salt additives of LiPF and LiNO in an ether solvent-based electrolyte can significantly improve the cycling stability and rate capability of a Li-carbon (Li-CNT) composite. As a result, an average cycling CE as high as 99.30% was obtained for the Li-CNT at a current density of 2.5 mA cm and an negative electrode to positive electrode capacity (N/P) ratio of 2. The cycling stability and rate capability enhancement of the Li-CNT negative electrode could be attributed to the formation of a better solid electrolyte interphase layer that contains both inorganic components and organic polyether. The former component mainly originates from the decomposition of the LiNO additive, while the latter comes from the LiPF-induced ring-opening polymerization of the ether solvent. This novel surface chemistry significantly improves the CE of Li negative electrode, revealing its importance for the practical application of lithium metal batteries.
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http://dx.doi.org/10.1007/s40820-021-00633-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053134PMC
April 2021

Statistical analysis for efficacy of tirofiban combined with ozagrel in the treatment of progressive cerebral infarction patients out of thrombolytic therapy time window.

Clinics (Sao Paulo) 2021;76:e2728. Epub 2021 Jun 14.

Department of Cardiovascular Medicine, ShanXi Bethune Hospital, Taiyuan, Shanxi Province, 030032, China.

Objectives: To investigate the safety and efficacy of combined tirofiban-ozagrel therapy for treating progressive stroke patients out of thrombolytic therapy time window.

Methods: This prospective, double-blind, randomized controlled study included 337 patients who had experienced an acute ischemic stroke between November 2017 and December 2018. All patients were randomized into three groups: 1) the tirofiban/ozagrel group (n=113), 2) the tirofiban group (n=110), and 3) the ozagrel group (n=114). The platelet aggregation (PAG), thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT), and fibrinogen (FIB) levels in the patients from these groups were evaluated before starting treatment and then, at 24h, 7 days, and 14 days after treatment. The National Institutes of Health Stroke Scale (NIHSS) scores were evaluated before treatment and then, 24h, 1 week, 2 weeks, and 4 weeks after treatment. The Barthel Index (BI) score was used to measure safety, and the modified Rankin scale (mRS) was used to evaluate disability following 3 months of treatment. The risk factors affecting clinical outcomes were analyzed using logistic multivariate regression.

Results: The mean NIHSS score for all the patients was 13.17±3.13 before treatment, and no significant difference between the basic clinical parameters of the three patient groups was found. Following treatment, both PAG and FIB were significantly reduced compared with the baseline (p<0.05). The levels of PAG and FIB in the tirofiban/ozagrel group were significantly lower than those in the tirofiban and ozagrel groups at 24h and 7 days after treatment (p<0.05). The NIHSS score decreased significantly in all treatment groups (p<0.05). The tirofiban/ozagrel NIHSS scores were significantly lower than that of the tirofiban and ozagrel groups at 24h, 1 week, and 2 weeks post initiation (p<0.05 for all). There were no significant differences in the BI and mRS scores or the intracranial hemorrhage rates; further, age, sex, Trial of ORG 10172 in acute stroke treatment (TOAST) type, baseline NIHSS and 24-h NIHSS scores, baseline thrombus-related factors, and treatment methods were shown to not be independent risk factors for clinical outcomes.

Conclusion: The combination of tirofiban and ozagrel, as well as monotherapy with either tirofiban or ozagrel, transiently improves the neural function of patients and reduces platelet aggregation and fibrinogen formation in the first 4 weeks following a stroke event; additionally, none of these treatments increased the risk for hemorrhage in these progressive stroke patients over a 3-month period.
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http://dx.doi.org/10.6061/clinics/2021/e2728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183337PMC
June 2021

Exosome-delivered miR-221/222 exacerbates tumor liver metastasis by targeting SPINT1 in colorectal cancer.

Cancer Sci 2021 Jun 14. Epub 2021 Jun 14.

Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University, Tianjin, China.

MicroRNAs (miRNAs) are involved in the progression of many cancers through largely unelucidated mechanisms. The results of our present study identified a gene cluster, miR-221/222, that is constitutively upregulated in serum exosome samples of patients with colorectal carcinoma (CRC) with liver metastasis (LM); this upregulation predicts a poor overall survival rate. Using an in vitro cell coculture model, we demonstrated that CRC exosomes harboring miR-221/222 activate liver hepatocyte growth factor (HGF) by suppressing SPINT1 expression. Importantly, miR-221/222 plays a key role in forming a favorable premetastatic niche (PMN) that leads to the aggressive nature of CRC, which was further shown through in vivo studies. Overall, our results show that exosomal miR-221/222 promotes CRC progression and may serve as a novel prognostic marker and therapeutic target for CRC with LM.
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http://dx.doi.org/10.1111/cas.15028DOI Listing
June 2021

Corrigendum: CDX2/mir-145-5p/SENP1 Pathways Affect LNCaP Cells Invasion and Migration.

Front Oncol 2021 18;11:685219. Epub 2021 May 18.

Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.

[This corrects the article DOI: 10.3389/fonc.2019.00477.].
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http://dx.doi.org/10.3389/fonc.2021.685219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8168537PMC
May 2021

Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine.

NPJ Sci Food 2021 Jun 1;5(1):12. Epub 2021 Jun 1.

School of Food and Biological Engineering, Hefei University of Technology, Hefei, China.

Due to the friendly temperature for virus survival, SARS-CoV-2 is frequently found in cold-chain foods, posing a serious threat to public health. Utilizing an interdigitated microelectrode chip modified with an antibody probe and integrating dielectrophoresis enrichment with interfacial capacitance sensing, a strategy is presented for the detection of trace level spike-protein from SARS-CoV-2. It achieves a limit of detection as low as 2.29 × 10 ng/mL in 20 s, with a wide linear range of 10-10 ng/mL and a selectivity of 234:1. The cost for a single test can be controlled to ~1 dollar. This strategy provides a competitive solution for real-time, sensitive, selective, and large-scale application in cold-chain food quarantine.
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http://dx.doi.org/10.1038/s41538-021-00094-3DOI Listing
June 2021

Coping with coronavirus disease 2019: Relationships between coping strategies, benefit finding and well-being.

Stress Health 2021 May 31. Epub 2021 May 31.

School of Psychological and Cognitive Sciences and Beijing Key Laboratory of Behavior and Mental Health, Peking University, Beijing, China.

The coronavirus disease 2019 (COVID-19) pandemic has caused both physical and psychological changes in the general public. The current study aimed to examine the relationship between well-being and coping strategies in response to the pandemic. Furthermore, we aimed to investigate the mediational role of benefit finding. A total of 521 participants aged 18-65 years were recruited from 29 regions of mainland China. Situation-specific coping strategies, including support seeking, personal hygiene practice and social distancing, were measured at Time 1. Benefit finding and well-being were assessed 1 month later. A multilevel mediation model was conducted with region included in level 2 as cluster ID. Support seeking and personal hygiene practice were positive predictors of benefit finding, which further mediated their relationships with well-being, while social distancing negatively predicted well-being. These results highlight the relationships of support seeking, personal hygiene practice and benefit finding with well-being during the COVID-19 pandemic. Our findings indicate that besides adopting adaptive coping strategies to prevent infection by COVID-19, individuals should be encouraged to recognize benefits associated with the COVID-19 outbreak.
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http://dx.doi.org/10.1002/smi.3072DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8237076PMC
May 2021

In vivo imaging via a red-emitting fluorescent probe to diagnosing liver cancer or drug-induced liver disease.

Anal Chim Acta 2021 Jul 6;1168:338621. Epub 2021 May 6.

Department of Radiology, Xinqiao Hospital, Army Medical University, Chongqing, 400037, PR China. Electronic address:

Leucine aminopeptidase (LAP) as an important proteolytic enzyme, has been mainly found in hepatobiliary cells, and overexpressed in hepatoma cells. Herein, a new highly selective red-emitting fluorescent probe (DCDHF-Ala) for LAP has been synthesized based on 2-dicyanomethyldiene-3-cyano-2, 5-dihydrofuran (DCDHF) as fluorophore, and alanine (Ala) as the detection group. More importantly, it's the first time to use Ala as a reactive group for LAP. DCDHF-Ala has a low detection limit (0.20 U/L), excellent water solubility and cell membrane permeability. In addition, the probe has been successfully applied to fluorescent imaging in cells and zebrafish. It's especially worth mentioning that, DCDHF-Ala has a high biosafety and enables a real-time detection of LAP levels in mice model. What's the most important is that DCDHF-Ala may be an effective tool to qualitatively monitor the upregulation of LAP induced by liver injury and liver cancer.
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http://dx.doi.org/10.1016/j.aca.2021.338621DOI Listing
July 2021

Mixture of controlled-release and conventional urea fertilizer application changed soil aggregate stability, humic acid molecular composition, and maize nitrogen uptake.

Sci Total Environ 2021 Oct 15;789:147778. Epub 2021 May 15.

National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Recourses and Environment, Shandong Agricultural University, Taian, Shandong 271018, China.

Controlled-release urea (CRU) fertilizer application has been shown to improve crop yield and nitrogen (N) use efficiency. However, its effects when mixed with conventional urea fertilizer on soil aggregate stability, humic acid (HA) molecular composition and crop N uptake remain unclear. Soil and plant samples were collected from a long-term (2008-2019) experiment on field maize (Zea mays L., 'Zhengdan 958') which included two types of fertilizers [conventional urea fertilizer (CUF), blended CUF with CRU fertilizer (CRF)], four N application rates (0, 150, 300 and 450 kg ha), each in three replicates. The results showed that at 300 kg N ha, compared to CUF treatment, the CRF treatment significantly improved soil aggregate characteristics [aggregate content with particle size larger than 0.25 mm (R) by 9.6%, mean weight diameter by 19.8%, and geometric mean diameter by 21.7%]. CRF treatment also increased HA content by 5.5%, fulvic acid (FA) by 5.5%, lignin-like molecules by 0.94 times, and protein-like molecules by 3.69 times. At grain-filling stage, CRF treatments significantly increased the sum of soil NH-N and NO-N content by 23.3-24.5%, sap bleeding rate by 12.8-18.2% and N delivery rate through bleeding sap by 60.6-87.7% compared to CUF treatments at the same N application rate. At the same rate of N application, the CRF treatments significantly improved the average yield during three growing seasons by 9.4-14.0% in contrast with CUF treatments. The regression equations showed that the maximum yield was 8294 kg ha for CUF at the application rate of 312 kg N ha while it was 9890 kg ha for CRF at the application rate of 286 kg N ha. We conclude that the long-term application of CRF changed the HA molecular structure, enhanced the water stable aggregates, improved crop N uptake, and increased economically viable maize yield.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147778DOI Listing
October 2021

A ratiometric fluorescent sensor for rapid detection of the pyroglutamate aminopeptidase-1 in mouse tumors.

J Mater Chem B 2021 Jun;9(22):4546-4554

Department of Radiology, Xinqiao Hospital, Army Medical University, Chongqing, 400037, P. R. China.

Pyroglutamate aminopeptidase-1 (PGP-1) is an important enzyme that plays an indispensable role in the process of inflammation. Up to now, few reports have been reported on the detection of PGP-1 activity in vivo and in vitro, and there are no reports on ratiometric detection. Here, the first red-emitting ratiometric fluorescent sensor (DP-1) for the specific detection of PGP-1 both in vivo and in vitro was designed and synthesized by using DCD-NH2 as the luminescent parent and pyroglutamate as a recognition group. After interacting with PGP-1, the amide bond is hydrolyzed by the enzyme and the color of the solution changes from yellow (λabs = 420 nm) to red (λabs = 520 nm), accompanied by obvious fluorescence emission wavelength change (from ∼564 nm to ∼616 nm). The probe has high specificity and sensitivity towards PGP-1 in about 10 min, and the DL is as low as 0.25 ng mL-1. Interestingly, under the stimulation of Freund's incomplete adjuvant and lipopolysaccharide, the imaging of DP-1 in HepG2 and RAW264 cells shows that the expression of PGP-1 is associated with inflammation. What's more, for the first, the imaging of a mouse tumor model confirms that the enzyme is closely related to the occurrence of some inflammation and tumor diseases. These results indicate that DP-1 can be used as an effective tool for real-time monitoring of PGP-1 levels both in vivo and in vitro and the study of inflammatory tumor pathology.
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http://dx.doi.org/10.1039/d1tb00372kDOI Listing
June 2021

HelPredictor models single-cell transcriptome to predict human embryo lineage allocation.

Brief Bioinform 2021 May 26. Epub 2021 May 26.

State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Sciences, Inner Mongolia University, Hohhot 010070, China.

The in-depth understanding of cellular fate decision of human preimplantation embryos has prompted investigations on how changes in lineage allocation, which is far from trivial and remains a time-consuming task by experimental methods. It is desirable to develop a novel effective bioinformatics strategy to consider transitions of coordinated embryo lineage allocation and stage-specific patterns. There are rapidly growing applications of machine learning models to interpret complex datasets for identifying candidate development-related factors and lineage-determining molecular events. Here we developed the first machine learning platform, HelPredictor, that integrates three feature selection methods, namely, principal components analysis, F-score algorithm and squared coefficient of variation, and four classical machine learning classifiers that different combinations of methods and classifiers have independent outputs by increment feature selection method. With application to single-cell sequencing data of human embryo, HelPredictor not only achieved 94.9% and 90.9% respectively with cross-validation and independent test, but also fast classified different embryonic lineages and their development trajectories using less HelPredictor-predicted factors. The above-mentioned candidate lineage-specific genes were discussed in detail and were clustered for exploring transitions of embryonic heterogeneity. Our tool can fast and efficiently reveal potential lineage-specific and stage-specific biomarkers and provide insights into how advanced computational tools contribute to development research. The source code is available at https://github.com/liameihao/HelPredictor.
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http://dx.doi.org/10.1093/bib/bbab196DOI Listing
May 2021

Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles.

J Extracell Vesicles 2021 May 21;10(7):e12093. Epub 2021 May 21.

Department of Cell and Molecular Biology QIMR Berghofer Medical Research Institute Brisbane Australia.

Urine is commonly used for clinical diagnosis and biomedical research. The discovery of extracellular vesicles (EV) in urine opened a new fast-growing scientific field. In the last decade urinary extracellular vesicles (uEVs) were shown to mirror molecular processes as well as physiological and pathological conditions in kidney, urothelial and prostate tissue. Therefore, several methods to isolate and characterize uEVs have been developed. However, methodological aspects of EV separation and analysis, including normalization of results, need further optimization and standardization to foster scientific advances in uEV research and a subsequent successful translation into clinical practice. This is written by the Urine Task Force of the Rigor and Standardization Subcommittee of ISEV consisting of nephrologists, urologists, cardiologists and biologists with active experience in uEV research. Our aim is to present the and identify challenges and gaps in current uEV-based analyses for clinical applications. Finally, recommendations for improved rigor, reproducibility and interoperability in uEV research are provided in order to facilitate advances in the field.
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http://dx.doi.org/10.1002/jev2.12093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138533PMC
May 2021

Diffusion weighted imaging to identify subcutaneous grease after a high-pressure injection injury: a case report.

J Hand Surg Eur Vol 2021 May 20:17531934211017401. Epub 2021 May 20.

Department of Radiology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, China.

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http://dx.doi.org/10.1177/17531934211017401DOI Listing
May 2021
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