Publications by authors named "Keli Chen"

53 Publications

DENV NS1 and MMP-9 cooperate to induce vascular leakage by altering endothelial cell adhesion and tight junction.

PLoS Pathog 2021 Jul 26;17(7):e1008603. Epub 2021 Jul 26.

Guangdong Provincial Key Laboratory of Virology, Institute of Medical Microbiology, Jinan University, Guangzhou, China.

Dengue virus (DENV) is a mosquito-borne pathogen that causes a spectrum of diseases including life-threatening dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Vascular leakage is a common clinical crisis in DHF/DSS patients and highly associated with increased endothelial permeability. The presence of vascular leakage causes hypotension, circulatory failure, and disseminated intravascular coagulation as the disease progresses of DHF/DSS patients, which can lead to the death of patients. However, the mechanisms by which DENV infection caused the vascular leakage are not fully understood. This study reveals a distinct mechanism by which DENV induces endothelial permeability and vascular leakage in human endothelial cells and mice tissues. We initially show that DENV2 promotes the matrix metalloproteinase-9 (MMP-9) expression and secretion in DHF patients' sera, peripheral blood mononuclear cells (PBMCs), and macrophages. This study further reveals that DENV non-structural protein 1 (NS1) induces MMP-9 expression through activating the nuclear factor κB (NF-κB) signaling pathway. Additionally, NS1 facilitates the MMP-9 enzymatic activity, which alters the adhesion and tight junction and vascular leakage in human endothelial cells and mouse tissues. Moreover, NS1 recruits MMP-9 to interact with β-catenin and Zona occludens protein-1/2 (ZO-1 and ZO-2) and to degrade the important adhesion and tight junction proteins, thereby inducing endothelial hyperpermeability and vascular leakage in human endothelial cells and mouse tissues. Thus, we reveal that DENV NS1 and MMP-9 cooperatively induce vascular leakage by impairing endothelial cell adhesion and tight junction, and suggest that MMP-9 may serve as a potential target for the treatment of hypovolemia in DSS/DHF patients.
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http://dx.doi.org/10.1371/journal.ppat.1008603DOI Listing
July 2021

Analysis of miRNA expression profile in lung tissues of an intermittent hypoxia rat model.

Respir Physiol Neurobiol 2021 Jul 14;294:103741. Epub 2021 Jul 14.

Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650101, China. Electronic address:

We screened key miRNAs in an intermittent hypoxia rat model and explored the biological roles of downstream target genes and related regulatory pathways. We analyzed the expression profile of miRNAs in the lung tissues of rats in the 5 % (IH1), 7.5 % (IH2), 10 % (IH3), 12.5 % (IH4) oxygen concentration and negative control (NC) groups and identified common miRNAs. Multiple differentially expressed miRNAs were detected, and intersection of their expression profiles yielded 10 common miRNAs with 929 target genes mainly distributed in the nucleus. Molecular functions pertained mainly to the activation of transcription factors, while biological processes focused on cell interaction and signal transduction. Among signaling pathways, the top 5 included the LKB1 signaling, nectin adhesion, and S1P pathways. 8 of 10 common miRNAs had excellent diagnostic value for detecting intermittent hypoxia. The miRNAs binds to the target gene might play a key role in the pathophysiological process of OSA through the LKB1/AMPK and S1P/Akt/eNOS signaling pathways.
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http://dx.doi.org/10.1016/j.resp.2021.103741DOI Listing
July 2021

Long-term copper exposure promotes apoptosis and autophagy by inducing oxidative stress in pig testis.

Environ Sci Pollut Res Int 2021 Jun 15. Epub 2021 Jun 15.

College of Veterinary, South China Agricultural University, Guangzhou, 510642, China.

Copper (Cu) is a heavy metal which is being used widely in the industry and agriculture. However, the overuse of Cu makes it a common environmental pollutant. In order to investigate the testicular toxicity of Cu, the pigs were divided into three groups and were given Cu at 10 (control), 125, and 250 mg/kg body weight, respectively. The feeding period was 80 days. Serum hormone results showed that Cu exposure decreased the concentrations of follicular stimulating hormone (FSH) and luteinizing hormone (LH) and increased the concentration of thyroxine (T4). Meanwhile, Cu exposure upregulated the expression of Cu transporter mRNA (Slc31a1, ATP7A, and ATP7B) in the testis, leading to increase in testicular Cu and led to spermatogenesis disorder. The Cu exposure led to an increased expression of antioxidant-related mRNA (Gpx4, TRX, HO-1, SOD1, SOD2, SOD3, CAT), along with increase in the MDA concentration in the testis. In LG group, the ROS in the testis was significantly increased. Furthermore, the apoptotic-related mRNA (Caspase3, Caspase8, Caspase9, Bax, Cytc, Bak1, APAF1, p53) and protein (Active Caspase3) and the autophagy-related mRNA (Beclin1, ATG5, LC3, and LC3B) expression increased after Cu exposure. The mitochondrial membrane potential in the testicular tissue decreased, while the number of apoptotic cells increased, as a result of oxidative stress. Overall, our study indicated that the Cu exposure promotes testicular apoptosis and autophagy by mediating oxidative stress, which is considered as the key mechanism causing testicular degeneration as well as dysfunction.
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http://dx.doi.org/10.1007/s11356-021-14853-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203493PMC
June 2021

A Purified Biflavonoid Extract From Alleviates Gout Arthritis via NLRP3/ASC/Caspase-1 Axis Suppression.

Front Pharmacol 2021 17;12:676297. Epub 2021 May 17.

Key Laboratory of Ministry of Education on Traditional Chinese Medicine Resource and Compound Prescription, Key Laboratory of Resources and Chemistry of Chinese Medicine, College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China.

Activation of nucleotide oligomerization domain-like receptor protein 3 (NLRP3) inflammasome plays a crucial role in gout. has been confirmed effective for the treatment of gout in hospital preparations. Flavonoids, such as amentoflavone (AM), are the main active components of this medicine. We aimed to investigate the flavonoid extract (TF) and AM's effects on NLRP3 inflammasome and their preventive effects on gout . LC-MS method was employed to investigate the chemical profile of TF. The cellular inflammation model was established by lipopolysaccharide (LPS) or monosodium urate (MSU) stimulation. The cell membrane integrality and morphological characteristics were determined by using Lactate dehydrogenase (LDH) assay kits, propidium iodide (PI) stain, and scanning electron microscopy (SEM). The inflammatory cytokines and NLRP3 inflammasome activation were determined using enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (RT-PCR), immunofluorescence staining, and western blotting. The acute gout mouse model was induced by MSU injection into footpads, and then the paw edema, inflammatory mediators, and histological examination (HE) were analyzed. The main constituents in TF are AM and robustaflavone. In the cellular inflammation model, TF down-regulated the levels of nitric oxide (NO), TNF-α, and LDH, suppressed NLRP3 inflammasome-derived interleukin-1β (IL-1β) secretion, decreased caspase-1 activation, repressed mature IL-1β expression, inhibited ASC speck formation and NLRP3 protein expression. In an acute gout mouse model, oral administration of TF to mice effectively alleviated paw edema, reduced inflammatory features, and decreased the levels of IL-1β in mouse foot tissue. Similarly, the characteristic constituent AM was also able to down-regulated the levels of NO, TNF-α, and LDH, down-regulate the mRNA expression of IL-1β, TNF-α, caspase-1, and NLRP3. Besides, the foot thickness, lymphocyte infiltration, and IL-1β level were also prevented by AM. The results indicated that TF and its main constituent AM alleviate gout arthritis via NLRP3/ASC/Caspase-1 axis suppression.
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http://dx.doi.org/10.3389/fphar.2021.676297DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165565PMC
May 2021

SARS-CoV-2 Nucleocapsid Protein Interacts with RIG-I and Represses RIG-Mediated IFN-β Production.

Viruses 2020 12 30;13(1). Epub 2020 Dec 30.

State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.

SARS-CoV-2 is highly pathogenic in humans and poses a great threat to public health worldwide. Clinical data shows a disturbed type I interferon (IFN) response during the virus infection. In this study, we discovered that the nucleocapsid (N) protein of SARS-CoV-2 plays an important role in the inhibition of interferon beta (IFN-β) production. N protein repressed IFN-β production induced by poly(I:C) or upon Sendai virus (SeV) infection. We noted that N protein also suppressed IFN-β production, induced by several signaling molecules downstream of the retinoic acid-inducible gene I (RIG-I) pathway, which is the crucial pattern recognition receptor (PRR) responsible for identifying RNA viruses. Moreover, our data demonstrated that N protein interacted with the RIG-I protein through the DExD/H domain, which has ATPase activity and plays an important role in the binding of immunostimulatory RNAs. These results suggested that SARS-CoV-2 N protein suppresses the IFN-β response through targeting the initial step, potentially the cellular PRR-RNA-recognition step in the innate immune pathway. Therefore, we propose that the SARS-CoV-2 N protein represses IFN-β production by interfering with RIG-I.
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http://dx.doi.org/10.3390/v13010047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823417PMC
December 2020

Paxillin mediates ATP-induced activation of P2X7 receptor and NLRP3 inflammasome.

BMC Biol 2020 11 26;18(1):182. Epub 2020 Nov 26.

Guangdong Provincial Key Laboratory of Virology, Institute of Medical Microbiology, Jinan University, Guangzhou, 510632, China.

Background: Extracellular adenosine triphosphate (ATP), a key danger-associated molecular pattern (DAMP) molecule, is released to the extracellular medium during inflammation by injured parenchymal cells, dying leukocytes, and activated platelets. ATP directly activates the plasma membrane channel P2X7 receptor (P2X7R), leading to an intracellular influx of K, a key trigger inducing NLRP3 inflammasome activation. However, the mechanism underlying P2X7R-mediated activation of NLRP3 inflammasome is poorly understood, and additional molecular mediators have not been identified. Here, we demonstrate that Paxillin is the molecule connecting the P2X7 receptor and NLRP3 inflammasome through protein interactions.

Results: We show a distinct mechanism by which Paxillin promotes ATP-induced activation of the P2X7 receptor and NLRP3 inflammasome. Extracellular ATP induces Paxillin phosphorylation and then facilitates Paxillin-NLRP3 interaction. Interestingly, Paxillin enhances NLRP3 deubiquitination and activates NLRP3 inflammasome upon ATP treatment and K efflux. Moreover, we demonstrated that USP13 is a key enzyme for Paxillin-mediated NLRP3 deubiquitination upon ATP treatment. Notably, extracellular ATP promotes Paxillin and NLRP3 migration from the cytosol to the plasma membrane and facilitates P2X7R-Paxillin interaction and PaxillinNLRP3 association, resulting in the formation of the P2X7R-Paxillin-NLRP3 complex. Functionally, Paxillin is essential for ATP-induced NLRP3 inflammasome activation in mouse BMDMs and BMDCs as well as in human PBMCs and THP-1-differentiated macrophages.

Conclusions: We have identified paxillin as a mediator of NLRP3 inflammasome activation. Paxillin plays key roles in ATP-induced activation of the P2X7 receptor and NLRP3 inflammasome by facilitating the formation of the P2X7R-Paxillin-NLRP3 complex.
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http://dx.doi.org/10.1186/s12915-020-00918-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694937PMC
November 2020

Alterations of the Gut Microbiome Composition and Lipid Metabolic Profile in Radiation Enteritis.

Front Cell Infect Microbiol 2020 21;10:541178. Epub 2020 Oct 21.

Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Radiation enteritis (RE) is a common complication in cancer patients receiving radiotherapy. Although studies have shown the changes of this disease at clinical, pathological and other levels, the dynamic characteristics of local microbiome and metabolomics are hitherto unknown. We aimed to examine the multi-omics features of the gut microecosystem, determining the functional correlation between microbiome and lipid metabolites during RE activity. By delivering single high-dose irradiation, a RE mouse model was established. High-throughput 16S rDNA sequencing and global lipidomics analysis were performed to examine microbial and lipidomic profile changes in the gut microecosystem. Spearman correlation analysis was used to determine the functional correlation between bacteria and metabolites. Clinical samples were collected to validate the above observations. During RE activity, the intestinal inflammation of the mice was confirmed by typical signs, symptoms, imaging findings and pathological evidences. 16S datasets revealed that localized irradiation dramatically altered the gut microbial composition, resulting in a decrease ratio of to . Lipidomics analysis indicated the remarkable lipidomic profile changes in enteric epithelial barrier, determining that glycerophospholipids metabolism was correlated to RE progression with the highest relevance. Spearman correlation analysis identified that five bacteria-metabolite pairs showed the most significant functional correlation in RE, including -PC(36:0e), -DG(18:0/20:4), -PC(35:2), -PC(35:6), and -TG(18:2/18:2/20:4). These observations were partly confirmed in human specimens. Our study provided a comprehensive description of microbiota dysbiosis and lipid metabolic disorders in RE, suggesting strategies to change local microecosystem to relieve radiation injury and maintain homeostasis.
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http://dx.doi.org/10.3389/fcimb.2020.541178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7609817PMC
June 2021

Material basis, effect, and mechanism of ethanol extract of Pinellia ternata tubers on oxidative stress-induced cell senescence.

Phytomedicine 2020 Oct 2;77:153275. Epub 2020 Jul 2.

Key Laboratory of Ministry of Education on Traditional Chinese Medicine Resource and Compound Prescription, Hubei Province Key Laboratory of Traditional Chinese Medicine Resource and Chemistry, College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, China. Electronic address:

Background: The tuber of Pinellia ternata has been used for a thousand years in China. P. ternata possessed the activities of anti-emetic, sedative-hypnotic, anti-cancer, anti-asthmatic, anti-tussive, and anti-inflammatory. It is the representative of expectorant medicines in Traditional Chinese Medicine (TCM). Phlegm is the pathological product and a new pathogenic factor of the metabolite, which is analogous to the damage of oxidative stress.

Purpose: The objectives of the study were to investigate the protective activity and mechanism of ethanol extract of P. ternata tubers (PTE) and its main constituents on oxidative stress-induced cell senescence.

Methods: HO and AAPH were used to establish cellular senescence models. The anti-aging effects of PTE and its components were evaluated by SA-β-gal staining, flow cytometry, scanning electron microscope (SEM), and multiple microplate reader, the molecular mechanisms of them were investigated by qRT-PCR and Western Blot.

Results: We found PTE exhibited the apparent effect on cell senescence, evidenced by inhibiting senescence β-Galactosidase (SA-β-gal) expression, lipofuscin accumulation, cell cycle arrest at the G2/M phase, oxidative damage and apoptosis, and increasing telomerase activity. Their mechanisms were related to increase expressions of SIRT1, forkhead box 3a (Foxo3a), Bcl-2, active regulator of SIRT1, RPS19BP1 (AROS), and Hu antigen R (HuR), but decrease Bax, p53 and deleted in breast cancer 1 (DBC1) levels. Furthermore, adenosine, and succinic acid, as the critical substances in PTE, could also inhibit SA-β-gal expression and cell cycle arrest, down-regulate the expression of Bax, and up-regulate Bcl-2, SirT1, and Foxo3a.

Conclusions: We have demonstrated that PTE slows down oxidative stress-induced cell senescence, and adenosine and succinic acid are the key active components.
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http://dx.doi.org/10.1016/j.phymed.2020.153275DOI Listing
October 2020

Fabrication and characterization of cellulose nanoparticles from maize stalk pith via ultrasonic-mediated cationic etherification.

Ultrason Sonochem 2020 Sep 17;66:104932. Epub 2019 Dec 17.

Faculty of Chemical Engineering, Kunming University of Science and Technology, Kunming, Yunnan 650500, China. Electronic address:

In this study, parenchyma cellulose, which was extracted from maize stalk pith as an abundant source of agricultural residues, was applied for preparing cellulose nanoparticles (CNPs) via an ultrasound-assisted etherification and a subsequent sonication process. The ultrasonic-assisted treatment greatly improved the modification of the pith cellulose with glycidyltrimethylammonium chloride, leading to a partial increase in the dissolubility of the as-obtained product and thus disintegration of sheet-like cellulose into nanoparticles. While the formation of CNPs by ultrasonication was largely dependent on the cellulose consistency in the cationic-modified system. Under the condition of 25% cellulose consistency, the longer sono-treated duration yielded a more stable and dispersible suspension of CNP due to its higher zeta potential. Degree of substitution and FT-IR analyses indicated that quaternary ammonium salts were grafted onto hydroxyl groups of cellulose chain. SEM and TEM images exhibited the CNP to have spherical morphology with an average dimeter from 15 to 55 nm. XRD investigation revealed that CNPs consisted mainly of a crystalline cellulose Ι structure, and they had a lower crystallinity than the starting cellulose. Moreover, thermogravimetric results illustrated the thermal resistance of the CNPs was lower than the pith cellulose. The optimal CNP with highly cationic charges, good stability and acceptable thermostability might be considered as one of the alternatively renewable reinforcement additives for nanocomposite production.
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http://dx.doi.org/10.1016/j.ultsonch.2019.104932DOI Listing
September 2020

Cohesive strength changes of sewer sediments during and after ultrasonic treatment: The significance of bound extracellular polymeric substance and microbial community.

Sci Total Environ 2020 Jun 19;723:138029. Epub 2020 Mar 19.

College of Environmental Science and Engineering, Tongji University, 200092 Shanghai, China; Key Laboratory of Yangtze River Water Environment, Ministry of Education, Tongji University, 200092 Shanghai, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China. Electronic address:

Sewer flushing is widely used to remove sewer sediment from drainage systems; however, its performance and cleaning efficiency are limited by the cohesive strength of sewer sediment. To address this, ultrasound, as a clean technology, is proposed to reduce the cohesive strength of sewer sediment. This study investigated the variations in the cohesive strength, extracellular polymeric substances (EPSs), and microbial community of sewer sediment with ultrasonic treatment. During ultrasonic conditioning, the degradation process of the cohesive strength followed the first-order kinetic model and was positively related to the degradation of bound-EPSs. Field emission scanning electron microscopy, particle diameter, and three-dimensional excitation emission analyses suggested that ultrasound reduced the cohesive strength by decreasing the bound-EPS concentration, which reduced the particle size of sewer sediment, and by destroying the structure of tryptophan proteins, which impaired the stability of agglomerated particles. Following ultrasonic treatment, the cohesive strength of the treated sediment was reduced to 69.3% of that of the raw sewer sediment after storage for 21 days; this result could be ascribed to the improvements in polysaccharide transport, amino acid transport, and the cell wall biogenesis functions of the microbial community, as indicated by PICRUSt. Furthermore, next-generation sequencing studies suggest that the proportions of Syntrophomonadaceae, Bacteroidetes_vadinHA17, Synergistaceae, and Syntrophaceae, which are associated with anaerobic digestion and methane production in sediment, improved conspicuously after ultrasonic conditioning.
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http://dx.doi.org/10.1016/j.scitotenv.2020.138029DOI Listing
June 2020

STING promotes NLRP3 localization in ER and facilitates NLRP3 deubiquitination to activate the inflammasome upon HSV-1 infection.

PLoS Pathog 2020 03 18;16(3):e1008335. Epub 2020 Mar 18.

Guangdong Key Laboratory of Virology, Institute of Medical Microbiology, Jinan University, Guangzhou, China.

One of the fundamental reactions of the innate immune responses to pathogen infection is the release of pro-inflammatory cytokines, including IL-1β, processed by the NLRP3 inflammasome. The stimulator of interferon genes (STING) has the essential roles in innate immune response against pathogen infections. Here we reveal a distinct mechanism by which STING regulates the NLRP3 inflammasome activation, IL-1β secretion, and inflammatory responses in human cell lines, mice primary cells, and mice. Interestingly, upon HSV-1 infection and cytosolic DNA stimulation, STING binds to NLRP3 and promotes the inflammasome activation through two approaches. First, STING recruits NLRP3 and facilitates NLRP3 localization in the endoplasmic reticulum, thereby facilitating the inflammasome formation. Second, STING interacts with NLRP3 and attenuates K48- and K63-linked polyubiquitination of NLRP3, thereby promoting the inflammasome activation. Collectively, we demonstrate that the cGAS-STING-NLRP3 signaling is essential for host defense against HSV-1 infection.
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http://dx.doi.org/10.1371/journal.ppat.1008335DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7080238PMC
March 2020

NS5 Conservative Site Is Required for Zika Virus to Restrict the RIG-I Signaling.

Front Immunol 2020 14;11:51. Epub 2020 Feb 14.

State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.

During host-virus co-evolution, cells develop innate immune systems to inhibit virus invasion, while viruses employ strategies to suppress immune responses and maintain infection. Here, we reveal that Zika virus (ZIKV), a re-emerging arbovirus causing public concerns and devastating complications, restricts host immune responses through a distinct mechanism. ZIKV nonstructural protein 5 (NS5) interacts with the host retinoic acid-inducible gene I (RIG-I), an essential signaling molecule for defending pathogen infections. NS5 subsequently represses K63-linked polyubiquitination of RIG-I, attenuates the phosphorylation and nuclear translocation of interferon regulatory factor 3 (IRF3), and inhibits the expression and production of interferon-β (IFN-β), thereby restricting the RIG-I signaling pathway. Interestingly, we demonstrate that the methyltransferase (MTase) domain of NS5 is required for the repression of RIG-I ubiquitination, IRF3 activation, and IFN-β production. Detailed studies further reveal that the conservative active site D146 of NS5 is critical for the suppression of the RIG-I signaling. Therefore, we uncover an essential role of NS5 conservative site D146 in ZIKV-mediated repression of innate immune system, illustrate a distinct mechanism by which ZIKV evades host immune responses, and discover a potential target for anti-viral infection.
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http://dx.doi.org/10.3389/fimmu.2020.00051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033454PMC
February 2021

Effect of carnosine supplementation on lipid profile, fasting blood glucose, HbA1C and insulin resistance: A systematic review and meta-analysis of long-term randomized controlled trials.

Complement Ther Med 2020 Jan 14;48:102241. Epub 2019 Nov 14.

Department of Endocrinology, Yiyang Central Hospital, Yiyang, Hunan, 413000, China. Electronic address:

Objective: Glucose disorders and dyslipidemia are closely associated with obesity and metabolic disease. The purpose of this study was to investigate the effect of Carnosine supplementation on lipid profile, fasting blood glucose, HbA1C and Insulin resistance.

Method: MEDLINE/PubMed, Scopus and Web of sciences were investigated to identify relevant articles up to June 2019. The search strategy combined the Medical Subject Heading and Title and/or abstract keywords. The combined effect sizes were calculated as weight mean difference (WMD) using the random-effects model. Between study heterogeneity was evaluated by the Cochran's Q test and I.

Results: Four RCTs studies investigated Carnosine use versus any control for at least 2 weeks were identified and analyzed. Overall results from the random-effects model on included studies, with 184 participants, indicated that carnosine intervention reduced HbA1C levels in intervention vs control groups (WMD: -0.92 %, 95 % CI: -1.20, -0.63, I:69 %). Four studies, including a total of 183 participants, reported TG changes as an outcome measure variable, but combined results did not show significant reduction in this outcome (WMD: -14.46 mg/dl, 95 % CI: -29.11, 0.19, I:94 %). Furthermore, combined results did not show any significant change in HOMA-IR, Cholesterol, fasting blood sugar, or HDL-C.

Conclusion: Carnosine supplementation results in a decrease in HbA1C, but elicits no effect on HOMA-IR, Cholesterol, fasting blood sugar, TG and HDL-C. Future studies with a larger sample sizes, varied doses of carnosine, and population-specific sub-groups are warranted to confirm, and enhance, the veracity of our findings.
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http://dx.doi.org/10.1016/j.ctim.2019.102241DOI Listing
January 2020

Virtual reality distraction decreases pain during daily dressing changes following haemorrhoid surgery.

J Int Med Res 2019 Sep 25;47(9):4380-4388. Epub 2019 Jul 25.

Huiqiao Medical Centre, Southern Hospital of Southern Medical University, Guangzhou, Guangdong Province, China.

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http://dx.doi.org/10.1177/0300060519857862DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6753557PMC
September 2019

Preparation and comparative assessment of regenerated cellulose films from corn (Zea mays) stalk pulp fines in DMAc/LiCl solution.

Carbohydr Polym 2019 Aug 30;218:315-323. Epub 2019 Apr 30.

Faculty of Chemical Engineering, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China.

In this study, cellulosic fines were separated from corn stalk soda-anthraquinone (AQ) pulp, then dissolved in DMAc/LiCl solvent system and regenerated to form films. Pulp fines was separated from pulp fibers using different standard sieves. The results exhibited that the value of specific surface area for fines was increased with the reduction of particle size. The cellulose of fines was successfully dissolved in DMAc/LiCl solution and regenerated as biopolymeric films in the water bath. Mechanical properties of regenerated cellulose films were enhanced with the increase of raw-material fineness from 120 to 300 mesh. It was exhibited a remarkable decrease on the values of tensile strength and maximum decomposition temperature of the regenerated cellulose film from 500-mesh fines, indicating that too small dimensions of cellulosic particles took disadvantages on physical strength and thermostability.
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http://dx.doi.org/10.1016/j.carbpol.2019.04.083DOI Listing
August 2019

Effects of extracellular polymeric substances and microbial community on the anti-scouribility of sewer sediment.

Sci Total Environ 2019 Oct 30;687:494-504. Epub 2019 May 30.

Institute of Groundwater and Earth Sciences, Jinan University, 510632 Guangzhou, China.

Sewer sediment is the main source of overflow pollution, and the anti-scouribility of sewer sediment directly determines the amount of the discharged contaminants. In this study, sewer sediments of different depths were collected from combined and storm sewers in Shanghai, China. The anti-scouribility, represented by the shear stress of each layer of sewer sediment, was detected in situ. The microbial community and extracellular polymeric substances (EPS), including carbohydrates and proteins present in the sewer sediments were characterized. The results indicated that the distribution of the anti-scouribility of sewer sediment is regulated. There were positive correlations between the content of EPS, proteins, and carbohydrates, and the anti-scouribility of sediments (Pearson Corr. = 0.604, sig. = 0.219; Pearson Corr. = 0.623, sig. = 0.234; Pearson Corr. = 0.727, sig. = 0.359, respectively). Furthermore, the microbial community had a positive influence on anti-scouribility. In particular, the gram-positive bacterial phyla of Bacteroidetes and Firmicutes may be important and influential for the improvement of anti-scouribility of sediment owing to their production of cellulose.
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http://dx.doi.org/10.1016/j.scitotenv.2019.05.387DOI Listing
October 2019

Improved thermal stability of regenerated cellulose films from corn (Zea mays) stalk pith using facile preparation with low-concentration zinc chloride dissolving.

Carbohydr Polym 2019 Aug 31;217:190-198. Epub 2018 Oct 31.

Faculty of Chemical Engineering, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China.

In this study, an improved facile cellulose dissolution with low chemical concentration process was used for preparing corn stalk pith regenerated cellulose (RC) films. The conventional method usually requires 68% zinc chloride solution for cellulose dissolution. The objective of this study is produced corn stalk pith RC films by using a low-concentration zinc chloride solution with improved thermal stability. The major weight loss peak temperature of RC films was increased from 231 °C to 307 °C as the crystallinity indexes increased from 48.30% to 53.19% by decreased the concentration of zinc chloride solution from 65% to 45%, respectively. The increased thermal stability can create new opportunities for the development of RC films dissolving by zinc chloride solution.
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http://dx.doi.org/10.1016/j.carbpol.2018.10.107DOI Listing
August 2019

Runoff simulation of two typical urban green land types with the Stormwater Management Model (SWMM): sensitivity analysis and calibration of runoff parameters.

Environ Monit Assess 2019 May 4;191(6):343. Epub 2019 May 4.

Key Laboratory of Yangtze River Water Environment, Ministry of Education, Tongji University, Shanghai, 200092, China.

The characteristics of surface runoff and the infiltration properties of urban green land are important to determine the effects of runoff reduction by low-impact development (LID) facilities. In this paper, two typical types of urban green land (lawn and shrub) in Shanghai were selected to study the runoff characteristics under eight rainfall events. The sensitivity of the runoff parameters was analyzed, and then, the optimal parameters were determined using the Stormwater Management Model (SWMM). The results showed that the interception and infiltration capacities of shrub were greater than those of lawn. The rainfall intensity and rainfall pattern were the major factors that influenced the interception and infiltration of rainwater. The threshold value that generates runoff varied across the eight rainfall events ranged from 1.6 to 28.5 mm for lawn and 4.5 to 32.0 mm for shrub. The maximum reduction ratios of runoff and peak flow for shrub were 52 and 57% higher than them for lawn, respectively. The parameters for shrub were more sensitive to runoff and peak flow compared with those for lawn. Under light rainfalls with a short duration, the maximum infiltration rate and depression storage were more sensitive than those under heavy rainfalls with a long duration. Antecedent dry weather period was not found to be a sensitive parameter except for the shrub under light rainfalls. The relative errors of runoff and dynamic mean runoff (60 min) for lawn and shrub were within ± 9.5%. The errors of peak flow ranged between - 21 and 16.6%. The dynamic runoff characteristics and the parameters for lawn and shrub determined in this study can provide references for simulating urban runoff and planning LID areas.
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http://dx.doi.org/10.1007/s10661-019-7445-9DOI Listing
May 2019

Super-reinforced photothermal stability of cellulose nanofibrils films by armour-type ordered doping Mg-Al layered double hydroxides.

Carbohydr Polym 2019 May 14;212:229-234. Epub 2019 Feb 14.

Faculty of Chemical Engineering, Kunming University of Science and Technology, Kunming 650500, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 30057, China. Electronic address:

A strategy to realize a reinforced photothermal stability cellulose nanofibrils (CNFs) films was reported. The LDHs were dispersed homogeneously onto the active site of CNFs surface by electrostatic self-assembly and the Mg/Al LDHs armour-type nanofibrils were prepared. After simple vacuum filtration, this kind nanofibrils can fabricate highly ordered multilayer film. Suitable amount of additive can bring outstanding photo-thermal property. The visible-light transmittance of the composite films can reach to c.a 80.0% when addition was 0.1% wt. and folding strength of the films contained 0.5% wt LDHs can increased by 15 times. The strength and the light transmittancevery of composite films was also higher than the pure nanocellulose film with the photothermal radiometry for about 30 h under 60 °C drying condition. The production is simple and highly repeatable process could be a good reference for preparation of high photothermal stability and transparent CNFs-based thin films.
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http://dx.doi.org/10.1016/j.carbpol.2019.01.063DOI Listing
May 2019

Histone methyltransferase SETDB1 promotes cells proliferation and migration by interacting withTiam1 in hepatocellular carcinoma.

BMC Cancer 2018 May 8;18(1):539. Epub 2018 May 8.

Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China.

Background: SETDB1 is a histone H3K9 methyltransferase, which plays a significant role in the occurrence and progression of tumors. Previous studies have confirmed that T-lymphom invasion and metastasis gene (Tiam1) is a protein associated with the metastasis of hepatocellular carcinoma (HCC); however, we have not yet been successful in elucidating the specific mechanism of HCC.

Methods: Yeast two-hybrid test was conducted to screen proteins that interacted with Tiam1 gene. Glutathione-S-transferase (GST) pull-down and crosslinking-immunoprecipitation (CLIP) assays were performed to determine whether SETDB1 can interact with Tiam1 gene. A series of related experiments were performed to explore role of SETDB1 on cell proliferation, migration, and invasion in HCC. Recovery experiment was performed to investigate the effect of Tiam1 knockdown on cell proliferation and migration, which was caused by SETDB1 overexpression in HCC cells. The expression of SETDB1 was frequently upregulated in HCC tissues and positively correlated with Tiam1.

Results: GST pull-down and CLIP assays were performed to elucidate the interaction between SETDB1 and Tiam1. Cell proliferation, migration, and epithelial mesenchymal transformation (EMT) in HCC cells was promoted with the overexpression of SETDB1. Following the knockdown of Tiam1 gene, the effect of SETDB1 on cell proliferation and migration was reversed in HCC cells. The expression of SETDB1 was frequently up-regulated in HCC tissues, and it was positively correlated with Tiam1 gene.

Conclusions: Ours is the first study to prove that SETDB1 promotes the proliferation and migration of cells by forming SETDB1-Tiam1 compounds. We found that SETDB1-Tiam1 compounds were involved in a novel pathway, which regulated epigenetic modification of gene expression in HCC samples.
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http://dx.doi.org/10.1186/s12885-018-4464-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5941371PMC
May 2018

Reinforcement of the bio-gas conversion from pyrolysis of wheat straw by hot caustic pre-extraction.

Biotechnol Biofuels 2018 19;11:72. Epub 2018 Mar 19.

Faculty of Chemical Engineering, Kunming University of Science and Technology, Kunming, 650500 Yunnan China.

Background: Pyrolysis has attracted growing interest as a versatile means to convert biomass into valuable products. Wheat straw has been considered to be a promising biomass resource due to its low price and easy availability. However, most of the products obtained from wheat straw pyrolysis are usually of low quality. Hot soda extraction has the advantage of selective dissolution of lignin whilst retaining the carbohydrates. This can selectively convert biomass into high-quality desired products and suppress the formation of undesirable products. The aim of this study was to investigate the pyrolysis properties of wheat straw under different hot caustic pretreatment conditions.

Results: Compared with the untreated straw, a greater amount of gas was released and fewer residues were retained in the extracted wheat straw, which was caused by an increase in porosity. When the NaOH loading was 14%, the average pore size of the extracted straw increased by 12% and the cumulative pore volume increased by 157% compared with the untreated straw. The extracted straw obtained from the 14% NaOH extraction was clearly selective for pyrolysis products. On one hand, many lignin pyrolysis products disappeared, and only four main lignin-unit-pyrolysis products were retained. On the other hand, polysaccharide pyrolysis products were enriched. Both propanone and furfural have outstanding peak intensities that could account for approximately 30% of the total pyrolysis products. However, with the excessive addition of NaOH (i.e. > 22% w/w) during pretreatment, the conversion of bio-gas products decreased. Thermogravimetric and low-temperature nitrogen-adsorption analysis showed that the pore structure had been seriously destroyed, leading to the closing of the release paths of the bio-gas and thus increasing the re-polymerisation of small bio-gas molecules.

Conclusions: After suitable extraction (14% NaOH loading extraction), a considerable amount (25%) of the soluble components dissolved out of the straw. This resulted in an increase in both pore size and volume. This condition appeared to be optimally selective for the release of value-added pyrolysis products such as furfural, ketones and lignin monomer units. However, excessive addition of alkali (22%) for extraction could change the original interior structure, resulting in a decrease in both pore size and volume. This interior structure modification limited the release of pyrolysis products, and greater carbonisation occurred.
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http://dx.doi.org/10.1186/s13068-018-1072-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5858128PMC
March 2018

Lidanpaidu prescription alleviates lipopolysaccharide-induced acute kidney injury by suppressing the NF-κB signaling pathway.

Biomed Pharmacother 2018 Mar;99:245-252

Key Laboratory of Education Ministry on Traditional Chinese Medicine Resource and Compound Prescription, Hubei University of Chinese Medicine, Wuhan 430065, China. Electronic address:

The Lidanpaidu Prescription (LDP), a hospital preparation, composed of Chinese classical preparations, has been reported to have antiendotoxin, anticoagulant and other effects. However, its therapeutic effect on lipopolysaccharide (LPS)-induced acute kidney injury (AKI) and the mechanisms remain unclear. Therefore, we administered LPD pretreatment at different doses to examine the protective effects and mechanisms in LPS-induced AKI in mice. The kidney injury induced by LPS was assessed by histological examination. ELISA was used to detect the levels of inflammatory cytokines. The mRNA expression of the inflammatory genes IKKβ and TNF-α in kidney tissues was assessed by RT-PCR. Finally, Western blot was performed to assess the NF-κB signaling pathway related proteins, and the nuclear translocation of NF-kB P65 was detected by immunofluorescence laser confocal microscopy. The findings suggested that LDP significantly improved at 48 h animal survival (66.7%), compared with the LPS group (26.7%), determined by a Kaplan-Meier analysis. LDP attenuated the kidney histopathological changes induced by LPS and decreased the inflammatory cytokine levels in serum and renal tissue. Moreover, LDP markedly inhibited the expression of inflammatory genes and suppressed the activation of relevant proteins in the nucleus. In summary, these findings suggest that LDP reduces LPS-induced AKI via a mechanism related to the suppression of the NF-κB signaling pathway.
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http://dx.doi.org/10.1016/j.biopha.2018.01.059DOI Listing
March 2018

Thermo-sensitive and swelling properties of cellouronic acid sodium/poly (acrylamide-co-diallyldimethylammonium chloride) semi-IPN.

Carbohydr Polym 2018 Feb 31;181:450-459. Epub 2017 Oct 31.

Faculty of Chemical Engineering, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.

In current study, cellouronic acid sodium (CAS), obtained from bagasse pith, has been introduced into poly(acrylamide-co-diallyldimethylammonium chloride) (poly(AM-co-DAC)) network to form novel thermo-sensitive semi-IPNs. The structure and morphology of the hydrogels were proved by Fourier transformation infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). The effects of CAS content, initiator charge, cross-linker dosage and swelling-medium property on the thermo-responsive water absorptivity were investigated in detail. The results elucidated that the prepared gels exhibited a thermo-sensibility with an upper critical solution temperature (UCST) and a high water-absorbency. And the values of UCST and equilibrium swelling ratio largely depended on the inner structure of the semi-IPNs and the external solvent property. It was also revealed that the swelling process conformed to the Schott's pseudo second order model and diffusion type was non-Fickian diffusion. The value of activation energy for this polyelectrolyte was found to be 8.74kJ/mol.
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http://dx.doi.org/10.1016/j.carbpol.2017.10.093DOI Listing
February 2018

Determination of zinc oxide content of mineral medicine calamine using near-infrared spectroscopy based on MIV and BP-ANN algorithm.

Spectrochim Acta A Mol Biomol Spectrosc 2018 Mar 5;193:133-140. Epub 2017 Dec 5.

Key Laboratory of Ministry of Education on Traditional Chinese Medicine Resource and Compound Prescription, & Hubei University of Chinese Medicine, Wuhan 430065, China. Electronic address:

Near-infrared (NIR) spectroscopy has been widely used in the analysis fields of traditional Chinese medicine. It has the advantages of fast analysis, no damage to samples and no pollution. In this research, a fast quantitative model for zinc oxide (ZnO) content in mineral medicine calamine was explored based on NIR spectroscopy. NIR spectra of 57 batches of calamine samples were collected and the first derivative (FD) method was adopted for conducting spectral pretreatment. The content of ZnO in calamine sample was determined using ethylenediaminetetraacetic acid (EDTA) titration and taken as reference value of NIR spectroscopy. 57 batches of calamine samples were categorized into calibration and prediction set using the Kennard-Stone (K-S) algorithm. Firstly, in the calibration set, to calculate the correlation coefficient (r) between the absorbance value and the ZnO content of corresponding samples at each wave number. Next, according to the square correlation coefficient (r) value to obtain the top 50 wave numbers to compose the characteristic spectral bands (4081.8-4096.3, 4188.9-4274.7, 4335.4, 4763.6,4794.4-4802.1, 4809.9, 4817.6-4875.4cm), which were used to establish the quantitative model of ZnO content using back propagation artificial neural network (BP-ANN) algorithm. Then, the 50 wave numbers were operated by the mean impact value (MIV) algorithm to choose wave numbers whose absolute value of MIV greater than or equal to 25, to obtain the optimal characteristic spectral bands (4875.4-4836.9, 4223.6-4080.9cm). And then, both internal cross and external validation were used to screen the number of hidden layer nodes of BP-ANN. Finally, the number 4 of hidden layer nodes was chosen as the best. At last, the BP-ANN model was found to enjoy a high accuracy and strong forecasting capacity for analyzing ZnO content in calamine samples ranging within 42.05-69.98%, with relative mean square error of cross validation (RMSECV) of 1.66% and coefficient of determination (R) of 95.75% in internal cross and relative mean square error of prediction (RMSEP) of 1.98%, R of 97.94% and ratio of performance to deviation (RPD) of 6.11 in external validation.
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http://dx.doi.org/10.1016/j.saa.2017.12.019DOI Listing
March 2018

Taxonomy and Identification of the Genus Scolopendra in China Using Integrated Methods of External Morphology and Molecular Phylogenetics.

Sci Rep 2017 11 22;7(1):16032. Epub 2017 Nov 22.

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, P.R. China.

The centipede Scolopendra has important medicinal value and high toxicity, making it to be an interesting subject for evolutionary studies. However, species identification in China is difficult because of limited resource exploration and lack of recent taxonomic revision. To improve the identification and taxonomy of the genus Scolopendra in China, an in-depth investigation was conducted, and an integrated method that combined morphological characteristics with molecular data was applied. The identification key was revised to show the main difference among species. Our results indicated that morphologically-delimited species were consistent with the molecular analysis inferred from the COI sequences with genetic distances and phylogenetic trees. Additional morphometrics of four characteristics provided criteria for shape variation. These results suggested that the members of the genus Scolopendra in China could be delineated as 14 separate species. A new species from Lufeng county, Yunnan province, was proposed according to its characteristics, which was named as S. lufengia sp. nov. Our results comprehensively ascertained the taxonomic status of Scolopendra species in China, explored their phylogenetic relationships, showed a high success in the identification of medicinal centipedes.
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http://dx.doi.org/10.1038/s41598-017-15242-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5700134PMC
November 2017

Histone Methyltransferase SETDB1 Promotes the Progression of Colorectal Cancer by Inhibiting the Expression of TP53.

J Cancer 2017 16;8(16):3318-3330. Epub 2017 Sep 16.

Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China.

SETDB1 is a novel histone methyltransferase associated with the functional tri-methylation of histone H3K9. Although aberrant high expression of SETDB1 was experimentally obversed in a variety of solid tumors, its underlying mechanisms in human carcinogenesis are not well known. In this study, we investigated the expression of SETDB1 in a large cohort of colorectal cancer (CRC) samples and cell lines for the first time. Our findings showed that SETDB1 was highly expressed in majority CRC tissues and cell lines; moreover, up-regulation of SETDB1 was negatively correlated with the survival rate of CRC patients. Functionally, over-expression of SETDB1 significantly promoted the proliferation and migration of CRC cells and , while knocking down SETDB1 suppressed their growth. Mechanistically, we showed that over-expression of SETDB1 significantly inhibited the apoptosis induced by 5-Fluorouracil in CRC cells, which was closely related to the inhibition of TP53 and BAX expression. Furthermore, we confirmed that SETDB1 could be recruited to the promoter region of TP53, which might contribute its inhibition of apoptosis. For conclusion, our study indicated that SETDB1 is essential for colorectal carcinogenesis, and may be a newly target for treatment and prognostic evaluation in CRC.
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http://dx.doi.org/10.7150/jca.20482DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5665049PMC
September 2017

Zinc finger protein 307 functions as a tumor‑suppressor and inhibits cell proliferation by inducing apoptosis in hepatocellular carcinoma.

Oncol Rep 2017 Oct 1;38(4):2229-2236. Epub 2017 Aug 1.

Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

Zinc finger protein 307 (ZNF307) is a new Krüppel-associated box zinc-finger protein gene and a member of the zinc-finger family of transcriptional factors. Notably, the role of ZNF307 and its underlying mechanisms involved in hepatocarcinogenesis are poorly investigated. In the present study, we found that the expression of ZNF307 was significantly downregulated in hepatocellular carcinoma (HCC) tissues, compared with that in adjacent non-tumor tissues. In vitro studies further demonstrated that ectopic expression of ZNF307 in HCC cell lines Bel7402 and HCCLM3 significantly reduced cell proliferation, migration and invasive ability. Concordantly, knockdown of ZNF307 increased cell proliferation, migration and invasive ability of HCC cell lines MHCC97L and QGY7701. In vivo functional studies showed that upregulation of ZNF307 expression in Bel7402 cells led to a suppression of tumorigenicity in mice, while knockdown of ZNF307 in MHCC97L cells resulted in reverse. effects. Importantly, flow cytometric analysis showed that ZNF307 overexpression increased the incidence of apoptosis, while ZNF307 knockdown decreased the incidence of apoptosis. Consistently, key regulators in apoptosis, such as caspase-3, BAX and BCL-2 were also regulated by ZNF307. Therefore, our results indicate that ZNF307 may serve as a tumor suppressor and inhibits cell proliferation of HCC via inducing apoptosis.
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http://dx.doi.org/10.3892/or.2017.5868DOI Listing
October 2017

Determination of Magnesium Oxide Content in Mineral Medicine Talcum Using Near-Infrared Spectroscopy Integrated with Support Vector Machine.

Appl Spectrosc 2017 Nov 21;71(11):2427-2436. Epub 2017 Sep 21.

1 Key Laboratory of Ministry of Education on Traditional Chinese Medicine Resource and Compound Prescription & Hubei University of Chinese Medicine, Wuhan, China.

In this research paper, a fast, quantitative, analytical model for magnesium oxide (MgO) content in medicinal mineral talcum was explored based on near-infrared (NIR) spectroscopy. MgO content in each sample was determined by ethylenediaminetetraacetic acid (EDTA) titration and taken as reference value of NIR spectroscopy, and then a variety of processing methods of spectra data were compared to establish a good NIR spectroscopy model. To start, 50 batches of talcum samples were categorized into training set and test set using the Kennard-Stone (K-S) algorithm. In a partial least squares regression (PLSR) model, both leave-one-out cross-validation (LOOCV) and training set validation (TSV) were used to screen spectrum preprocessing methods from multiplicative scatter correction (MSC), and finally the standard normal variate transformation (SNV) was chosen as the optimal pretreatment method. The modeling spectrum bands and ranks were optimized using PLSR method, and the characteristic spectrum ranges were determined as 11995-10664, 7991-6661, and 4326-3999 cm, with four optimal ranks. In the support vector machine (SVM) model, the radical basis function (RBF) kernel function was used. Moreover, the full spectrum data of samples pretreated with SNV, the characteristic spectrum data screened using synergy interval partial least squares (SiPLS), and the scoring data of the first four ranks obtained by a partial least squares (PLS) dimension reduction of characteristic spectrum were taken as input variables of SVM, and the MgO content reference values of various sample were taken as output values. In addition, the SVM model internal parameters were optimized using the grid optimization method (GRID), particle swarm optimization (PSO), and genetic algorithm (GA) so that the optimal C and g-values were determined and the validation model was established. By comprehensively comparing the validation effects of different models, it can be concluded that the scoring data of the first four ranks obtained by PLS dimension reduction of characteristic spectrum were taken as input variables of SVM, and the PLS-SVM regression model established using GRID was the optimal NIR spectroscopy quantitative model of talc. This PLS-SVM regression model (rank = 4) measured that the MgO content of talcum was in the range of 17.42-33.22%, with root mean square error of cross validation (RMSECV) of 2.2127%, root mean square error of calibration (RMSEC) of 0.6057%, and root mean square error of prediction (RMSEP) of 1.2901%. This model showed high accuracy and strong prediction capacity, which can be used for rapid prediction of MgO content in talcum.
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http://dx.doi.org/10.1177/0003702817727016DOI Listing
November 2017

Different erosion characteristics of sediment deposits in combined and storm sewers.

Water Sci Technol 2017 Apr;75(7-8):1922-1931

College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China E-mail:

To investigate the different erosion patterns of sediments in combined and storm sewers, sediments from three separate sewer systems and two combined sewer systems in urban Shanghai were collected for the flushing experiments. These experiments were conducted with different consolidation periods and shear velocities. As the consolidation period increases, dissolved oxygen exhibits a positive effect on the microbial transformations of organic substrates. Potential structural changes and separations of the surface and bottom layers of sediments are observed. The results also reveal that the organic matter, particle size and moisture have different effects on the erosion resistance of sediments. Furthermore, illicit connections behaved as an important factor affecting the viscosity and static friction force of particles, which directly alter the erosion resistance of sewer sediments.
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http://dx.doi.org/10.2166/wst.2017.076DOI Listing
April 2017

A novel lncRNA uc.134 represses hepatocellular carcinoma progression by inhibiting CUL4A-mediated ubiquitination of LATS1.

J Hematol Oncol 2017 04 19;10(1):91. Epub 2017 Apr 19.

Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.

Background: Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide, and tumor recurrence and metastasis are major factors that contribute to the poor outcome of patients with HCC. Long noncoding RNAs (lncRNAs) are known to regulate different tumorigenic processes, and a growing body of evidence indicates that Hippo kinase signaling is inactivated in many cancers. However, the upstream lncRNA regulators of Hippo kinase signaling in HCC are poorly understood.

Methods: Using a lncRNA microarray, we identified a novel lncRNA, uc.134, whose expression was significantly decreased in the highly aggressive HCC cell line HCCLM3 compared with MHCC97L cells. Furthermore, we evaluated uc.134 expression in clinical samples using in situ hybridization (ISH) and quantitative real-time polymerase chain reaction (qRT-PCR) analysis. The full-length transcript of uc.134 was confirmed using rapid amplification of cDNA ends (RACE) analyses. To investigate the biological function of uc.134, we performed gain-of-function and loss-of-function studies both in vitro and in vivo. The underlying mechanisms of uc.134 in HCC were investigated using RNA pulldown, RNA immunoprecipitation, ubiquitination assays, Western blotting, mRNA microarray analyses, and qRT-PCR analyses.

Results: The ISH assay revealed that uc.134 expression was significantly decreased in 170 paraffin-embedded samples from patients with HCC compared with adjacent tissues and uc.134 expression directly correlated with patient prognosis. Furthermore, we defined a 1867-bp full-length transcript of uc.134 using 5'- and 3'-RACE analysis. The overexpression of uc.134 inhibited HCC cell proliferation, invasion, and metastasis in vitro and in vivo, whereas the knockdown of uc.134 produced the opposite results. Furthermore, we confirmed that uc.134 (1408-1867 nt) binds to CUL4A (592-759 aa region) and inhibits its nuclear export. Moreover, we demonstrated that uc.134 inhibits the CUL4A-mediated ubiquitination of LATS1 and increases YAP phosphorylation to silence the target genes of YAP. Finally, a positive correlation between uc.134, LATS1, and pYAP was confirmed in 90 paraffin-embedded samples by ISH and immunohistochemical staining.

Conclusions: Our study identifies that a novel lncRNA, uc.134, represses hepatocellular carcinoma progression by inhibiting the CUL4A-mediated ubiquitination of LATS1 and increasing YAP phosphorylation. The use of this lncRNA may offer a promising treatment approach by inhibiting YAP and activating Hippo kinase signaling.
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http://dx.doi.org/10.1186/s13045-017-0449-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5395742PMC
April 2017
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