Keiro Uchino

Keiro Uchino

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Keiro Uchino

Keiro Uchino

Publications by authors named "Keiro Uchino"

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Refinement of ectopic protein expression through the GAL4/UAS system in Bombyx mori: application to behavioral and developmental studies.

Sci Rep 2017 09 18;7(1):11795. Epub 2017 Sep 18.

Division of Life Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa, 920-1192, Japan.

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http://dx.doi.org/10.1038/s41598-017-12102-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603595PMC
September 2017

Precise genome editing in the silkworm Bombyx mori using TALENs and ds- and ssDNA donors - A practical approach.

Insect Biochem Mol Biol 2016 11 25;78:29-38. Epub 2016 Aug 25.

Biology Centre of the ASCR, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic.

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http://dx.doi.org/10.1016/j.ibmb.2016.08.006DOI Listing
November 2016

Transgenic Expression of the piRNA-Resistant Masculinizer Gene Induces Female-Specific Lethality and Partial Female-to-Male Sex Reversal in the Silkworm, Bombyx mori.

PLoS Genet 2016 08 31;12(8):e1006203. Epub 2016 Aug 31.

Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa-shi, Chiba 277-8562, Japan.

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http://dx.doi.org/10.1371/journal.pgen.1006203DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5007099PMC
August 2016

A Hox Gene, Antennapedia, Regulates Expression of Multiple Major Silk Protein Genes in the Silkworm Bombyx mori.

J Biol Chem 2016 Mar 26;291(13):7087-96. Epub 2016 Jan 26.

From the Transgenic Silkworm Research Unit, National Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki 305-8634, Japan,

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http://www.jbc.org/lookup/doi/10.1074/jbc.M115.699819
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http://dx.doi.org/10.1074/jbc.M115.699819DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4807290PMC
March 2016

Establishment of tools for neurogenetic analysis of sexual behavior in the silkmoth, Bombyx mori.

PLoS One 2014 14;9(11):e113156. Epub 2014 Nov 14.

Transgenic Silkworm Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0113156PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4232604PMC
December 2015

Construction and long term preservation of clonal transgenic silkworms using a parthenogenetic strain.

J Insect Physiol 2015 Oct 23;81:28-35. Epub 2015 Jun 23.

Biology Centre CAS, 370 05 České Budějovice, Czech Republic. Electronic address:

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http://dx.doi.org/10.1016/j.jinsphys.2015.06.011DOI Listing
October 2015

Targeted disruption of a single sex pheromone receptor gene completely abolishes in vivo pheromone response in the silkmoth.

Sci Rep 2015 Jun 5;5:11001. Epub 2015 Jun 5.

Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.

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http://dx.doi.org/10.1038/srep11001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4457163PMC
June 2015

Cell proliferation by silk gut incorporating FGF-2 protein microcrystals.

Sci Rep 2015 Jun 8;5:11051. Epub 2015 Jun 8.

1] Department of Applied Biology, Kyoto Institute of Technology, Sakyo-ku, Kyoto 606-8585, Japan [2] Insect Biomedical Centre, Kyoto Institute of Technology, Sakyo-ku, Kyoto 606-8585, Japan.

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http://dx.doi.org/10.1038/srep11051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459171PMC
June 2015

Fluorescence imaging for a noninvasive in vivo toxicity-test using a transgenic silkworm expressing green fluorescent protein.

Sci Rep 2015 Jun 10;5:11180. Epub 2015 Jun 10.

Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 111-0033, Japan.

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http://dx.doi.org/10.1038/srep11180DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462092PMC
June 2015

Effect of ATG initiation codon context motifs on the efficiency of translation of mRNA derived from exogenous genes in the transgenic silkworm, Bombyx mori.

Springerplus 2014 10;3:136. Epub 2014 Mar 10.

Transgenic Silkworm Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8634 Japan.

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http://dx.doi.org/10.1186/2193-1801-3-136DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4320137PMC
February 2015

LIM-homeodomain transcription factor Awh is a key component activating all three fibroin genes, fibH, fibL and fhx, in the silk gland of the silkworm, Bombyx mori.

Insect Biochem Mol Biol 2015 Jan 18;56:29-35. Epub 2014 Nov 18.

Graduate School of Life Science, Hokkaido University, Japan; Division of Biological Sciences and Center for Genome Dynamics, Faculty of Science, Hokkaido University, North 10, West 8, Kita-ku, Sapporo, Hokkaido 060-0810, Japan. Electronic address:

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https://linkinghub.elsevier.com/retrieve/pii/S09651748140017
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http://dx.doi.org/10.1016/j.ibmb.2014.11.003DOI Listing
January 2015

Transgenic silkworms expressing human insulin receptors for evaluation of therapeutically active insulin receptor agonists.

Biochem Biophys Res Commun 2014 Dec 4;455(3-4):159-64. Epub 2014 Nov 4.

Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 111-0033, Japan. Electronic address:

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http://dx.doi.org/10.1016/j.bbrc.2014.10.143DOI Listing
December 2014

Odorant concentration differentiator for intermittent olfactory signals.

J Neurosci 2014 Dec;34(50):16581-93

Graduate School of Information Science and Technology, University of Tokyo, Tokyo, 113-8656, Japan, Research Center for Advanced Science and Technology, University of Tokyo, Tokyo, 153-8904, Japan,

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http://dx.doi.org/10.1523/JNEUROSCI.2319-14.2014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6608502PMC
December 2014

Identification of a novel strong and ubiquitous promoter/enhancer in the silkworm Bombyx mori.

G3 (Bethesda) 2014 May 28;4(7):1347-57. Epub 2014 May 28.

Transgenic Silkworm Research, National Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki 305-8634, Japan

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http://dx.doi.org/10.1534/g3.114.011643DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4455783PMC
May 2014

Hormonal regulation and developmental role of Krüppel homolog 1, a repressor of metamorphosis, in the silkworm Bombyx mori.

Dev Biol 2014 Apr 4;388(1):48-56. Epub 2014 Feb 4.

National Institute of Agrobiological Sciences, Ohwashi 1-2, Tsukuba, Ibaraki 305-8634, Japan. Electronic address:

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http://dx.doi.org/10.1016/j.ydbio.2014.01.022DOI Listing
April 2014

Hox transcription factor Antp regulates sericin-1 gene expression in the terminal differentiated silk gland of Bombyx mori.

Dev Biol 2014 Feb 12;386(1):64-71. Epub 2013 Dec 12.

Graduate School of Life Science, Hokkaido University, Japan; Division of Biological Sciences and Center for Genome Dynamics, Faculty of Science, Hokkaido University, North 10, West 8, Kita-ku, Sapporo, Hokkaido 060-0810, Japan. Electronic address:

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http://dx.doi.org/10.1016/j.ydbio.2013.12.002DOI Listing
February 2014

Pheromone responsiveness threshold depends on temporal integration by antennal lobe projection neurons.

Proc Natl Acad Sci U S A 2013 Sep 4;110(38):15455-60. Epub 2013 Sep 4.

Department of Advanced Interdisciplinary Studies, Graduate School of Engineering, and Research Center for Advanced Science and Technology, University of Tokyo, Meguro-ku, Tokyo 153-8904, Japan.

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http://dx.doi.org/10.1073/pnas.1313707110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3780863PMC
September 2013

A visible dominant marker for insect transgenesis.

Nat Commun 2012 ;3:1295

Transgenic Silkworm Research Unit, National Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki 305-8634, Japan.

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http://dx.doi.org/10.1038/ncomms2312DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3535423PMC
June 2013

Tightly controlled tetracycline-inducible transcription system for explosive gene expression in cultured silkworm cells.

Arch Insect Biochem Physiol 2013 Apr 31;82(4):173-82. Epub 2013 Jan 31.

Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki, Fukuoka, Japan.

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http://dx.doi.org/10.1002/arch.21083DOI Listing
April 2013

CD36 homolog divergence is responsible for the selectivity of carotenoid species migration to the silk gland of the silkworm Bombyx mori.

J Lipid Res 2013 Feb 16;54(2):482-95. Epub 2012 Nov 16.

Division of Radiological Protection and Biology, National Institute of Infectious Diseases, Shinjuku Tokyo 162-8640, Japan.

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http://dx.doi.org/10.1194/jlr.M032771DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3588874PMC
February 2013

Efficient disruption of endogenous Bombyx gene by TAL effector nucleases.

Insect Biochem Mol Biol 2013 Jan 8;43(1):17-23. Epub 2012 Nov 8.

Institute of Entomology, Biology Centre ASCR, and Faculty of Science, University of South Bohemia, 37005 Ceske Budejovice, Czech Republic.

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https://linkinghub.elsevier.com/retrieve/pii/S09651748120016
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http://dx.doi.org/10.1016/j.ibmb.2012.10.011DOI Listing
January 2013

SID-1 protein of Caenorhabditis elegans mediates uptake of dsRNA into Bombyx cells.

Insect Biochem Mol Biol 2012 Feb 8;42(2):148-54. Epub 2011 Dec 8.

Transgenic Silkworm Research Unit, National Institute of Agrobiological Sciences, 1-2 Ohwashi, Tsukuba, Ibaraki 305-8634, Japan.

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http://dx.doi.org/10.1016/j.ibmb.2011.11.007DOI Listing
February 2012

An efficient binary system for gene expression in the silkworm, Bombyx mori, using GAL4 variants.

Arch Insect Biochem Physiol 2011 Apr 19;76(4):195-210. Epub 2011 Jan 19.

Transgenic Silkworm Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.

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http://dx.doi.org/10.1002/arch.20402DOI Listing
April 2011

Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection.

Insect Biochem Mol Biol 2010 Oct 6;40(10):759-65. Epub 2010 Aug 6.

Biology Centre, Academy of Sciences and the Faculty of Natural Sciences, University of South Bohemia, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic.

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http://dx.doi.org/10.1016/j.ibmb.2010.07.012DOI Listing
October 2010

Efficient soluble protein production on transgenic silkworms expressing cytoplasmic chaperones.

Appl Microbiol Biotechnol 2010 Aug 23;87(6):2147-56. Epub 2010 May 23.

Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, Fukuoka, Japan.

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http://dx.doi.org/10.1007/s00253-010-2617-0DOI Listing
August 2010

Construction of a binary transgenic gene expression system for recombinant protein production in the middle silk gland of the silkworm Bombyx mori.

Transgenic Res 2010 Jun 30;19(3):473-87. Epub 2009 Sep 30.

Transgenic Silkworm Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305-8634, Japan.

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http://link.springer.com/10.1007/s11248-009-9328-2
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http://dx.doi.org/10.1007/s11248-009-9328-2DOI Listing
June 2010

Identification of Ser2 proteins as major sericin components in the non-cocoon silk of Bombyx mori.

Insect Biochem Mol Biol 2010 Apr 1;40(4):339-44. Epub 2010 Mar 1.

National Institute of Agrobiological Sciences, Tsukuba, Japan.

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http://dx.doi.org/10.1016/j.ibmb.2010.02.010DOI Listing
April 2010

The silkworm mutant lemon (lemon lethal) is a potential insect model for human sepiapterin reductase deficiency.

J Biol Chem 2009 Apr 26;284(17):11698-705. Epub 2009 Feb 26.

Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.

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http://dx.doi.org/10.1074/jbc.M900485200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2670173PMC
April 2009

GFP labeling of neurosecretory cells with the GAL4/UAS system in the silkmoth brain enables selective intracellular staining of neurons.

Zoolog Sci 2008 May;25(5):509-16

Research Center for Advanced Science and Technology, University of Tokyo, Tokyo, Japan.

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http://dx.doi.org/10.2108/zsj.25.509DOI Listing
May 2008

A new method for the modification of fibroin heavy chain protein in the transgenic silkworm.

Biosci Biotechnol Biochem 2007 Dec 7;71(12):2943-51. Epub 2007 Dec 7.

National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.

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http://dx.doi.org/10.1271/bbb.70353DOI Listing
December 2007

Improving cell-adhesive properties of recombinant Bombyx mori silk by incorporation of collagen or fibronectin derived peptides produced by transgenic silkworms.

Biomacromolecules 2007 Nov 16;8(11):3487-92. Epub 2007 Oct 16.

Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo, Japan.

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http://pubs.acs.org/doi/abs/10.1021/bm700646f
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http://dx.doi.org/10.1021/bm700646fDOI Listing
November 2007

Carotenoid silk coloration is controlled by a carotenoid-binding protein, a product of the Yellow blood gene.

Proc Natl Acad Sci U S A 2007 May 11;104(21):8941-6. Epub 2007 May 11.

Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.

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http://dx.doi.org/10.1073/pnas.0702860104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1885607PMC
May 2007