Publications by authors named "Kai Zhou"

430 Publications

Constructing highly active Co sites in Prussian blue analogues for boosting electrocatalytic water oxidation.

Chem Commun (Camb) 2021 Jul 21. Epub 2021 Jul 21.

College of Physics and State Key Laboratory of Coal Mine Disaster Dynamics and Control, Chongqing University, Chongqing 401331, China. and Analytical and Testing Center, Chongqing University, Chongqing 401331, China.

High-valence cobalt sites are considered as highly active centers for the oxygen evolution reaction (OER) and their corresponding construction is thus of primary importance in the pursuit of outstanding performance. Herein, we report the design and facile synthesis of abundant high-valence cobalt sites by introducing Zn2+ into CoFe Prussian blue analogues (PBAs). The modification results in the drastic morphological transformation from a pure phase (CoFe-PBA) to a three-phase composite (CoFeZn-PBA), with a significant increase not only the amount of highly oxidized Co sites but the specific surface area (by up to 4 times). Moreover, the obtained sample also exhibits outstanding electric conductivity. Consequently, an excellent OER performance with an overpotential of 343 [email protected] mA cm-2 and a Tafel slope of 75 mV dec-1 was achieved in CoFeZn-PBA, which outperforms the commercial IrO2 catalyst. Further analysis reveals that CoFeZn-PBA becomes (oxyhydr)oxides after the OER.
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http://dx.doi.org/10.1039/d1cc02224eDOI Listing
July 2021

Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging.

Chem Sci 2021 Jul 18;12(25):8660-8667. Epub 2021 May 18.

Department of Life Sciences, Pohang University of Science and Technology Pohang 37673 Republic of Korea

Multicolor fluorescence imaging is a powerful tool visualizing the spatiotemporal relationship among biomolecules. Here, we report that commonly employed organic dyes exhibit a blue-conversion phenomenon, which can produce severe multicolor image artifacts leading to false-positive colocalization by invading predefined spectral windows, as demonstrated in the case study using EGFR and Tensin2. These multicolor image artifacts become much critical in localization-based superresolution microscopy as the blue-converted dyes are photoactivatable. We provide a practical guideline for the use of organic dyes for multicolor imaging to prevent artifacts derived by blue-conversion.
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http://dx.doi.org/10.1039/d1sc00612fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246296PMC
July 2021

Uncovering phylogenetic relationships and genetic diversity of water dropwort using phenotypic traits and SNP markers.

PLoS One 2021 6;16(7):e0249825. Epub 2021 Jul 6.

Institute of Vegetables, Wuhan Academy of Agricultural Sciences, Wuhan, Hubei, China.

The water dropworts Oenanthe linearis Wall. ex DC. and O. javanica (Blume) DC. are aquatic perennial herbs that have been used in China as vegetables and traditional medicines. However, their phylogenetic relationships and genetic diversity are poorly understood. Here, we presented the phenotypic traits and genome-wide DNA marker-based analysis of 158 water dropwort accessions representing both species. The analysis revealed that Oenanthe linearis was readily segregated into linear-leaf and deep-cleft leaf water dropworts according to their leaf shapes at flowering. Oenanthe javanica was classified by clustering analysis into two clusters based mainly on the morphological characteristics of their ultimate segments (leaflets). A set of 11 493 high-quality single-nucleotide polymorphisms was identified and used to construct a phylogenetic tree. There was strong discrimination between O. linearis and O. javanica, which was consistent with their phenotype diversification. The population structure and phylogenetic tree analyses suggested that the O. linearis accessions formed two major groups, corresponding to the linear-leaf and deep-cleft leaf types. The most obvious phenotypic differences between them were fully expressed at the reproductive growth stage. A single-nucleotide polymorphism-based analysis revealed that the O. javanica accessions could be categorized into groups I andII. However, this finding did not entirely align with the clusters revealed by morphological classification. Landraces were clustered into one group along with the remaining wild accessions. Hence, water dropwort domestication was short in duration. The level of genetic diversity for O. linearis (π = 0.1902) was slightly lower than that which was estimated for O. javanica (π = 0.2174). There was a low level of genetic differentiation between O. linearis and O. javanica (Fst = 0.0471). The mean genetic diversity among accessions ranged from 0.1818 for the linear-leaf types to 0.2318 for the groupII accessions. The phenotypic traits and the single-nucleotide polymorphism markers identified here lay empirical foundation for future genomic studies on water dropwort.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0249825PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8259969PMC
July 2021

Application of Combined Prediction Model Based on Core and Coritivity Theory in Continuous Blood Pressure Prediction.

Comb Chem High Throughput Screen 2021 Jul 4. Epub 2021 Jul 4.

School of Mathematics Physics and Statistics, Shanghai University of Engineering Science, Shanghai 201620, China.

Background And Objective: Blood pressure is vital evidence for clinicians to predict diseases and check the curative effect of diagnosis and treatment. To further improve the prediction accuracy of blood pressure, this paper proposes a combined prediction model of blood pressure based on coritivity theory and photoplethysmography.

Method: First of all, we extract eight features of photoplethysmogram, followed by using eight machine learning prediction algorithms such as K-nearest neighbor, classification and regression trees and random forest to predict systolic blood pressure. Secondly, aiming at the problem of sub-model selection of combination forecasting model, from the point of graph theory, we construct an undirected network graph G, the results of each single prediction model constitute a vertex set. If the maximum mutual information coefficient between vertices is greater than or equal to 0.69, the vertices are connected by edges. The maximum core of graph G is a submodel of the combinatorial model.

Results: According to the definition of core and coritivity, the maximum core of G is random forest regression and Gaussian kernel support vector regression model. The results show that the SDP estimation error of the combined prediction model based on random forest regression and Gaussian kernel support vector regression is 3.56 ±5.28mmhg, which is better than other single models and meets the AAMI standards.

Conclusion: The combined model determined by core and coritivity has higher prediction performance for blood pressure.
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http://dx.doi.org/10.2174/1386207324666210705113121DOI Listing
July 2021

Atomically Dispersed Platinum Modulated by Sulfide as an Efficient Electrocatalyst for Hydrogen Evolution Reaction.

Adv Sci (Weinh) 2021 Jun 13;8(12):2100347. Epub 2021 May 13.

Faculty of Materials and Manufacturing Key Laboratory of Advanced Functional Materials Education Ministry of China Beijing University of Technology Beijing 100124 P. R. China.

Catalytically active metals atomically dispersed on supports presents the ultimate atom utilization efficiency and cost-effective pathway for electrocatalyst design. Optimizing the coordination nature of metal atoms represents the advanced strategy for enhancing the catalytic activity and the selectivity of single-atom catalysts (SACs). Here, we designed a transition-metal based sulfide-NiS with abundant exposed Ni vacancies created by the interaction between chloride ions and the functional groups on the surface of Ni3S2 for the anchoring of atomically dispersed Pt (Pt-NiS). The theoretical calculation reveals that unique Pt-NiS support interaction increases the orbital electron occupation at the Fermi level and leads to a shift-down of the -band center, which energetically enhances HO adsorption and provides the optimum H binding sites. Introducing Pt into Ni position in NiS system can efficiently enhance electronic field distribution and construct a metallic-state feature on the Pt sites by the orbital hybridization between S-3p and Pt-5d for improved reaction kinetics. Finally, the fabricated Pt-NiS SAC is supported by Ag nanowires network to construct a seamless conductive three-dimensional (3D) nanostructure ([email protected] NWs), and the developed catalyst shows an extremely great mass activity of 7.6 A mg with 27-time higher than the commercial Pt/C HER catalyst.
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http://dx.doi.org/10.1002/advs.202100347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224416PMC
June 2021

Eleven routine clinical features predict COVID-19 severity uncovered by machine learning of longitudinal measurements.

Comput Struct Biotechnol J 2021 17;19:3640-3649. Epub 2021 Jun 17.

Shaoxing People's Hospital, Shaoxing Hospital, Zhejiang University School of Medicine, Shaoxing 312000, Zhejiang Province, China.

Severity prediction of COVID-19 remains one of the major clinical challenges for the ongoing pandemic. Here, we have recruited a 144 COVID-19 patient cohort, resulting in a data matrix containing 3,065 readings for 124 types of measurements over 52 days. A machine learning model was established to predict the disease progression based on the cohort consisting of training, validation, and internal test sets. A panel of eleven routine clinical factors constructed a classifier for COVID-19 severity prediction, achieving accuracy of over 98% in the discovery set. Validation of the model in an independent cohort containing 25 patients achieved accuracy of 80%. The overall sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were 0.70, 0.99, 0.93, and 0.93, respectively. Our model captured predictive dynamics of lactate dehydrogenase (LDH) and creatine kinase (CK) while their levels were in the normal range. This model is accessible at https://www.guomics.com/covidAI/ for research purpose.
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http://dx.doi.org/10.1016/j.csbj.2021.06.022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225590PMC
June 2021

The transcription factor RUNX2 fuels YAP1 signaling and gastric cancer tumorigenesis.

Cancer Sci 2021 Jun 23. Epub 2021 Jun 23.

Department of Cancer Center, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Despite considerable efforts in the detection and treatment of gastric cancer (GC), the underlying mechanism of the progression of GC remains unknown. Our previous work has demonstrated the remarkable role of Runt-related transcription factor 2 (RUNX2), in fueling the invasion and metastasis of GC. The present study aimed to elucidate the role of RUNX2 in tumorigenesis of GC. We assessed Runx2 expression and its clinical significance via bioinformatic analysis of the Cancer Genome Atlas and Gene Expression Omnibus databases. Roles for Runx2 in self-renewal and tumorigenesis were examined in vitro and in vivo. Further bioinformatic analysis was applied to study the mechanism of GC progression. We found that Runx2 was highly expressed in the early stage of GC and positively correlated with a poor clinical outcome of patients. Runx2 was also significantly correlated with clinicopathological features, such as Hp infection, new neoplastic events, primary therapeutic outcome, ethnicity, race, and tumor stage. Multivariate analysis revealed that together with Runx2, age, cancer status, M stage, and T stage were independent prognostic factors for the outcome of GC patients. RUNX2 overexpression induced increased anchorage-independent colony formation, sphere formation, and tumorigenesis in GC cells in vitro and in vivo. Mechanistically, bioinformatic analysis indicated that yes1 associated transcriptional regulator (YAP1) might be a downstream target of RUNX2. Specific knockdown of YAP1 reduced the tumor-initiating ability of GC cells induced by ectopic Runx2 expression. Our findings support the hypothesis that RUNX2 exerts oncogenic properties via YAP1 regulation, highlighting essential roles for RUNX2 and YAP1 in gastric carcinogenesis and suggesting potential therapeutic targets.
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http://dx.doi.org/10.1111/cas.15045DOI Listing
June 2021

Glutathione-mediated formation of disulfide bonds modulates the properties of myofibrillar protein gels at different temperatures.

Food Chem 2021 Jun 12;364:130356. Epub 2021 Jun 12.

Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230601, China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China. Electronic address:

The present study illustrated modulation of protein aggregation by affecting disulfide/sulfhydryl exchange reactions by adding different concentrations of free thiol represented by reduced-glutathione (GSH) for modulating myofibrillar protein (MP) gel properties at 75 °C or 95 °C. Gel strength and rheological results showed the effects of GSH were dependent on the concentrations (5, 10, 20, 40, and 80 g/kg) and heating temperatures. SEM results showed that the addition of GSH improved the gel microstructure at 95 °C. AFM and DLS results indicated that protein aggregation was also inhibited. At 75 °C, the addition of GSH influenced both MP aggregation and gel properties. Low concentrations (5, 10 g/kg) of GSH promoted aggregation, whereas high concentrations (20, 40, and 80 g/kg) of GSH inhibited this. By analyzing the protein structure and cross-linking pattern changes of MP and MP/GSH composites, a pathway involving GSH influencing MP gel properties was determined.
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http://dx.doi.org/10.1016/j.foodchem.2021.130356DOI Listing
June 2021

Exosomale microRNA-21 Promotes Keloid Fibroblast Proliferation and Collagen Production by inhibiting Smad7.

J Burn Care Res 2021 Jun 19. Epub 2021 Jun 19.

Department of Plastic and Burn Surgery, West China School of Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

In keloid fibroblasts, microRNA-21 (miR-21) enhances activation of the TGF-β-Smad-signaling pathway by downregulating Smad7 expression, thereby promoting keloid fibroblast proliferation and collagen production. However, it is unclear whether miR-21 performs the above-mentioned functions through exosomal transport. Here, we extracted exosomes from the culture supernatants of keloid and normal skin fibroblasts, and observed that exosomes from both cell types secreted exosomes; however, keloid fibroblasts secreted significantly more exosomal miR-21 than normal skin fibroblasts (P < 0.001). Interestingly, we also observed that exosomal miR-21 could enter target keloid fibroblasts. In addition, inhibiting exosomal miR-21 upregulated Smad7 protein expression and reduced Smad2 and Smad3 protein levels in target keloid fibroblasts. Furthermore, inhibiting exosomal miR-21 downregulated collagen I and collagen III expression in target keloid fibroblasts, increased the proportion of apoptotic cells, and reduced cell proliferation. Taken together, these results show that exosomal miR-21 promoted proliferation and collagen production in keloid fibroblasts by inhibiting Smad7. Thus, we identified regulatory roles for miR-21 in promoting keloid fibroblast proliferation and participating in keloid formation and development. These findings imply that miR-21 may serve as a novel target for controlling the development of keloids.
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http://dx.doi.org/10.1093/jbcr/irab116DOI Listing
June 2021

Platinum single-atom catalyst coupled with transition metal/metal oxide heterostructure for accelerating alkaline hydrogen evolution reaction.

Nat Commun 2021 Jun 18;12(1):3783. Epub 2021 Jun 18.

Faculty of Materials and Manufacturing, Beijing University of Technology, Beijing, P. R. China.

Single-atom catalysts provide an effective approach to reduce the amount of precious metals meanwhile maintain their catalytic activity. However, the sluggish activity of the catalysts for alkaline water dissociation has hampered advances in highly efficient hydrogen production. Herein, we develop a single-atom platinum immobilized NiO/Ni heterostructure (Pt-NiO/Ni) as an alkaline hydrogen evolution catalyst. It is found that Pt single atom coupled with NiO/Ni heterostructure enables the tunable binding abilities of hydroxyl ions (OH*) and hydrogen (H*), which efficiently tailors the water dissociation energy and promotes the H* conversion for accelerating alkaline hydrogen evolution reaction. A further enhancement is achieved by constructing Pt-NiO/Ni nanosheets on Ag nanowires to form a hierarchical three-dimensional morphology. Consequently, the fabricated Pt-NiO/Ni catalyst displays high alkaline hydrogen evolution performances with a quite high mass activity of 20.6 A mg for Pt at the overpotential of 100 mV, significantly outperforming the reported catalysts.
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http://dx.doi.org/10.1038/s41467-021-24079-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213696PMC
June 2021

Aberrant Methylation of Aging-Related Genes in Asthma.

Front Mol Biosci 2021 25;8:655285. Epub 2021 May 25.

Department of Respiratory Medicine, National Clinical Research Center for Respiratory Diseases, Xiangya Hospital, Central South University, Changsha, China.

Asthma is a complex pulmonary inflammatory disease which is common among older adults. Aging-related alterations have also been found in structural cells and immune cells of asthma patients. Nonetheless, the underlying mechanism by which differenced aging-related gene contributes to asthma pathology remains unclear. Of note, DNA methylation (DNAm) has been proven to play a critical mechanism for age-related gene expression changes. However, the methylation changes of aging-related genes in asthma patients are still obscure. First, changes in DNAm and gene expression were detected with multiple targeted bisulfite enrichment sequencing (MethTarget) and qPCR in peripheral blood of 51 healthy controls (HCs) and 55 asthmatic patients. Second, the correlation between the DNAm levels of specific altered CpG sites and the pulmonary function indicators of asthma patients was evaluated. Last, the receiver operator characteristic (ROC) curve and principal component analysis (PCA) were used to identify the feasibility of the candidate CpG sites as biomarkers for asthma. Compared with HCs, there was a differential mRNA expression for nine aging-related genes in peripheral blood of asthma patients. Besides, the methylation levels of the nine aging-related genes were also altered in asthma patients, and a total of 68 CpG sites were associated with the severity of asthma. Notably, 9 of the 68 CpG sites were significantly associated with pulmonary function parameters. Moreover, ROC curve and PCA analysis showed that the candidate differential methylation sites (DMSs) can be used as potential biomarkers for asthma. In summary, this study confirmed the differentially expressed mRNA and aberrant DNAm level of aging-related genes in asthma patients. DMSs are associated with the clinical evaluation indicators of asthma, which indicate the involvement of aging-related genes in the pathogenesis of asthma and provide some new possible biomarkers for asthma.
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http://dx.doi.org/10.3389/fmolb.2021.655285DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203316PMC
May 2021

Identification, pyramid and candidate genes of QTLs for associated traits based on a dense erect panicle rice CSSL-Z749 and five SSSLs, three DSSLs and one TSSL.

Rice (N Y) 2021 Jun 16;14(1):55. Epub 2021 Jun 16.

Rice Research Institute, Academy of Agricultural Sciences, Southwest University, 400715, Chongqing, PR China.

Background: Seed-set density is an important agronomic trait in rice. However, its genetic mechanism is complex. Chromosome segment substitution lines (CSSLs) are ideal materials for studying complex traits.

Results: A rice CSSL, Z749, with a dense and erect panicle phenotype, was identified among progeny of the recipient parent Nipponbare and the donor parent Xihui 18. Z749 carried seven substitution segments (average length 2.12 Mb). Compared with Nipponbare, Z749 showed significant increases in the numbers of primary (NPB) and secondary branches (NSB), number of spikelets (SPP) and grains per panicle (GPP), seed-set density (SSD), and decrease in panicle length (PL). A secondary F population derived from a cross between Nipponbare and Z749 was used to map quantitative trait loci (QTLs) for associated traits. Fifteen QTLs distributed on chromosomes 5, 7, 8, and 10 were detected. The QTL qPL7 might be an allele of OsFAD8 and the remaining 14 QTLs (e.g., qSSD5 and qSSD10 etc.) might be novel. Fourteen QTLs were verified using five single-segment substitution lines (SSSLs). The seed-set density of Z749 was controlled predominantly by one major QTL (qSSD10) and two minor QTLs (qSSD5 and qSSD8). The QTLs qSSD10, qSSD5, and qSSD8 were fine-mapped to intervals of 1.05, 1.46, and 1.53 Mb on chromosomes 10, 5, and 8, respectively. Analysis of QTL additive effects indicated that qSSD5, qSSD8, and qSSD10 from Xihui18 increased seed-set density of Z749 by 14.10, 11.38, and 5.11 spikelets per 10 cm panicle, respectively. Analysis of QTL epistatic effects revealed that pyramiding of qSSD5 and qSSD8, qSSD5 and qSSD10, qSSD8 and qSSD10, and qSSD5, qSSD8 and qSSD10 produced novel genotypes with increased seed-set density.

Conclusions: Inheritance of seed-set density in Z749 was controlled predominantly by one major QTL (qSSD10) and two minor QTLs (qSSD5 and qSSD8). Then, they were fine-mapped to intervals of 1.05, 1.46, and 1.53 Mb on chromosomes 10, 5, 8, respectively. Two MAPK genes (OsMPK9 and OsMPK17) and one gene (candidate gene 6) involved in auxin metabolism might be candidate genes for qSSD5, and OsSAUR32 might be the candidate gene for qSSD8. Pyramiding of qSSD5, qSSD8, and qSSD10 enhanced seed-set density.
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http://dx.doi.org/10.1186/s12284-021-00496-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208356PMC
June 2021

Identification of TYR, TYRP1, DCT and LARP7 as related biomarkers and immune infiltration characteristics of vitiligo via comprehensive strategies.

Bioengineered 2021 12;12(1):2214-2227

Department of Dermatology, the First People's Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan, China.

This study aims to explore biomarkers associated with vitiligo and analyze the pathological role of immune cell infiltration in the disease. We used the robust rank aggregation (RRA) method to integrate three vitiligo data sets downloaded from gene expression omnibus database, identify the differentially expressed genes (DEGs) and analyze the functional correlation. Then, the comprehensive strategy of combined weighted gene coexpression network analysis (WGCNA) and logical regression of the selection operator (LASSO), support vector machine recursive feature elimination (SVM-RFE), and random forest (RF) machine learning algorithm are employed to screen and biomarkers associated with vitiligo. Finally, the immune cell infiltration of vitiligo was evaluated by CIBERSORT, and the correlation between biomarkers and infiltrating immune cells was analyzed. Herein, we identified 131 robust DEGs, and enrichment analysis results showed that robust DEGs and melanogenesis were closely associated with vitiligo development and progression. TYR, TYRP1, DCT and LARP7 were identified as vitiligo-related biomarkers. Immune infiltration analysis demonstrated that CD4 T Cell, CD8 T Cell, Tregs, NK cells, dendritic cells, and macrophages were involved in vitiligo's pathogenesis. In summary, we adopted a comprehensive strategy to screen biomarkers related to vitiligo and explore the critical role of immune cell infiltration in vitiligo.: TYR, Tyrosinase; TYRP1, Tyrosinase-related protein-1; DCT, dopachrome tautomerase; LARP7, La ribonucleoprotein domain family, member-7; RRA, robust rank aggregation; DEGs, differentially expressed genes; WGCNA, weighted gene coexpression network analysis; LASSO, logical regression of the selection operator; SVM-RFE, support vector machine recursive feature elimination; RF, random forest; GWAS, Genome-wide association study; FasL, Fas-Fas ligand; Tregs, T-regulatory cells; NK, natural killer; GEPCs, gene expression profiling chips; GO, gene ontology; GSEA, gene set enrichment analysis; FDR, false discovery rate; AUC, area under the curve; ROC, receiver-operating characteristic; BP, biological process; CC, cellular component; MF, molecular function.
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http://dx.doi.org/10.1080/21655979.2021.1933743DOI Listing
December 2021

Development of a One-Step Multiplex PCR Assay for Differential Detection of Four species (, , , and ) Belonging to Complex With Clinical Significance.

Front Cell Infect Microbiol 2021 18;11:677089. Epub 2021 May 18.

Shenzhen Institute of Respiratory Diseases, Second Clinical Medical College, Jinan University (Shenzhen People's Hospital); The First Affiliated Hospital, Southern University of Science and Technology (Shenzhen People's Hospital), Shenzhen, China.

complex (ECC) is composed of multiple species and the taxonomic status is consecutively updated. In last decades ECC is frequently associated with multidrug resistance and become an important nosocomial pathogen. Currently, rapid and accurate identification of ECC to the species level remains a technical challenge, thus impedes our understanding of the population at the species level. Here, we aimed to develop a simple, reliable, and economical method to distinguish four epidemiologically prevalent species of ECC with clinical significance, i.e., , , , and . A total of 977 ECC genomes were retrieved from the GenBank, and unique gene for each species was obtained by core-genome comparisons. Four pairs of species-specific primers were designed based on the unique genes. A total of 231 ECC clinical strains were typed both by typing and by species-specific PCRs. The specificity and sensitivity of the four species-specific PCRs ranged between 96.56% and 100% and between 76.47% and 100%, respectively. The PCR for showed the highest specificity and sensitivity. A one-step multiplex PCR was subsequently established by combining the species-specific primers. Additional 53 -typed ECC and 20 non-ECC isolates belonging to six species obtained from samples of patients, sewage water and feces of feeding animals were tested by the multiplex PCR. The identification results of both techniques were concordant. The multiplex PCR established in this study provides an accurate, expeditious, and cost-effective way for routine diagnosis and molecular surveillance of ECC strains at species level.
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http://dx.doi.org/10.3389/fcimb.2021.677089DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169972PMC
July 2021

A Comparison of Safety and Effectiveness Between Wingspan and Neuroform Stents in Patients With Middle Cerebral Artery Stenosis.

Front Neurol 2021 20;12:527541. Epub 2021 May 20.

Department of Neurology, Xinqiao Hospital, Army Medical University, Chongqing, China.

Percutaneous transluminal angioplasty and stenting with the Wingspan stent has proven safe and effective in patients with middle cerebral artery stenosis (MCAS), but the off-label use of the Neuroform stent might be an alternative treatment. This study aimed to compare the safety and effectiveness of the above two intracranial stents in patients with MCAS. We retrospectively analyzed consecutive patients with symptomatic MCAS who had been treated with the Neuroform EZ or the Wingspan stent. A propensity score was generated to control for differences in baseline characteristics. The endpoints were the rate of peri-procedural complications within 30 days after stenting, the in-stent restenosis rate, and any target-vessel-related stroke or deaths during follow-up. After matching for propensity score, the peri-procedural complication rate in the Wingspan group was 7.4% compared with 5.6% in the Neuroform group ( = 1.00), while the follow-up in-stent restenosis rates were 23.3 vs. 14.3%, respectively ( = 0.41). In the restenosis group, the patients tended to be younger ( < 0.01) and the degree of artery stenosis before stenting was higher ( < 0.01). This study indicated that in patients with symptomatic MCAS, Neuroform EZ stents are an alternative to Wingspan. Moreover, younger age and higher degree of artery stenosis before stenting might be a risk factor of in-stent restenosis.
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http://dx.doi.org/10.3389/fneur.2021.527541DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177007PMC
May 2021

Proton therapy for craniopharyngioma in adults: a protocol for systematic review and meta-analysis.

BMJ Open 2021 06 1;11(6):e046043. Epub 2021 Jun 1.

Department of Neurosurgery, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China

Introduction: Craniopharyngioma is the most challenging to treat brain tumour with high recurrence rates, which can be effectively reduced by adjuvant radiotherapy. In recent years, proton therapy (PT), with its physical properties of heavy ion beam, that is, Prague peak phenomenon, has been more frequently used in patients with craniopharyngioma. Compared with conventional X-ray beam radiotherapy, PT can reduce the damage to normal tissues and enlarge the damage to tumours. Some studies have shown that PT has advantages in the treatment of craniopharyngioma in adults. However, the optimal management of craniopharyngioma remains controversial. The purpose of this study was to evaluate the efficacy and safety of PT for craniopharyngioma in adults.

Methods And Analysis: We will search six databases (MEDLINE, EMBASE, Web of Science, the Cochrane Library, Amed, Scopus), clinical research registration websites and grey literature, aiming to identify randomised controlled trials (RCTs) on PT for craniopharyngioma in adults between 1 January 1954 and 28 September 2021. In the RCTs, PT will be used as the intervention group, and conventional X-ray beam radiotherapy will be used as the comparator group. Tumour recurrence and survival will be the primary outcome, and treatment-related toxicity will be the secondary outcome. The study selection, data extraction, bias risk and quality evaluation will be operated by two to four researchers independently. We will use Review Manager V.5.2 (RevMan V.5.2) for data analysis. If there is significant heterogeneity, we will identify the source of heterogeneity by subgroup analysis.

Ethics And Dissemination: Our study is based on existing RCTs and does not require ethical approval. The results of the study will be published in a peer-reviewed journal or at a related conference.

Prospero Registration Number: CRD42020200909.
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http://dx.doi.org/10.1136/bmjopen-2020-046043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173282PMC
June 2021

Identification and treatment of a cervical sinus tract in a patient with 10 years of infertility.

Fertil Steril 2021 May 27. Epub 2021 May 27.

Department of Gynecology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, People's Republic of China. Electronic address:

Objective: To introduce a special case of endometrial cavity fluid (ECF), highlighting the application of hysteroscopy and laparoscopic surgical techniques in the treatment of cervical sinus tract.

Design: Narrated video featuring the diagnosis and surgical management of a case of recurrent ECF. Informed consent was obtained from the patient, and approval was granted by the ethics committee of the First Affiliated Hospital of the Wenzhou Medical University.

Setting: Academic tertiary hospital.

Patient(s): A 36-year-old woman, gravida 0, had menstrual spotting for 13 years after abdominal myomectomy of a 104 × 86 × 111-mm myoma on the posterior uterine wall near the cervix. She failed to conceive after her marriage for 10 years, and 5 operations, including hysteroscopy and laparoscopy, were performed to increase pregnancy opportunities. She also underwent in vitro fertilization and embryo transfer procedures many times, but failed. Transvaginal sonography preoperatively suggested that ECF sometimes appeared and sometimes disappeared. The local echo of the posterior wall of the cervix was enhanced. A 40-mm cystic dark area was found beside the right ovary, which seemed to connect with the cervical hyperechoic part. Additionally, a solid mass of the right adnexa with abundant blood supply was detected.

Intervention(s): First, hysteroscopy was performed to explore the ECF. A deep and narrow cervical sinus with a steady stream of accumulated blood overflowed in the lower part of the cervix, and a normal uterine cavity was found. Laparoscopic adhesiolysis and enucleation of the cystic structure that connected to the sinus tract then were performed. Hysteroscopy was repeated to determine the thinnest cervical region by the light transmission test. A horizontal incision was made on the thinnest layer. Scar tissues were removed. The incision was sutured in full layer intermittently and continuously under laparoscopy. The postoperative thickness of the muscular layer in the sinus was confirmed by light transmission test of hysteroscopy. The patient was discharged on the third day after operation, uneventfully. Histopathologic examination showed that the cystic structure and scar tissue contained smooth muscle tissue and were covered by both mucinous columnar epithelium of the cervical canal and endometrial glandular epithelium.

Main Outcome Measure(s): Restoration of normal anatomy, removal of uterine effusion, and symptomatic relief.

Result(s): At the 6-month follow-up, the patient's menstrual cycles returned to normal without the recurrence of menstrual spotting. The ultrasound scan also showed a symmetrical uterus without ECF.

Conclusion(s): Patients with ECF who underwent assisted reproductive surgeries were related to the poor prognosis. However, the treatment should be different according to the causes, appearance time, and accumulation amount, including expectant treatment, postponement of embryo transfer, transvaginal aspiration, laparoscopic salpingectomy, or proximal tubal occlusion. For patients with recurrent ECF and/or special appearance on ultrasound, endoscopic examination is necessary. In addition, patients with large myomas at difficult locations required a uniform strategy to reduce the intraoperative and postoperative complications, especially for the nulligravida women.
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http://dx.doi.org/10.1016/j.fertnstert.2021.05.074DOI Listing
May 2021

The influence of sagittal pelvic malrotation on transverse acetabular ligament guided cup orientation: a retrospective cohort study.

BMC Musculoskelet Disord 2021 May 28;22(1):495. Epub 2021 May 28.

Department of Orthopedics, Orthopedic Research Institute, West China Hospital, Sichuan University, 37# Guoxue Rd, 610041, Chengdu, China.

Background: Total hip arthroplasty (THA) candidates frequently present pelvic malrotation. The aim of this study is to analyze how pelvic malrotation influence transverse acetabular ligament (TAL) guided cup orientation and investigate whether pelvic malrotation produce different clinical outcomes after THA.

Methods: We retrospectively reviewed a consecutive series of THA patients (144 hips) who use TAL as a guidance for cup positioning from March 2017 to January 2020. The patients were divided into normal pelvis (NP) group and backward pelvis (BP) group by sagittal pelvic malrotation assessed by APPA, the angle between the vertical and the APP on standing lateral pelvic radiographs preoperatively. Cup anteversion and inclination and that out of the safe zones were measured and compared in two groups. The demographic data, clinical results, and complications of patients were also compared.

Results: Backward pelvic malrotation were found in 60.6 % of this cohort of THA candidates. The mean angle of both inclination and anteversion in BP group were significantly larger than that in NP group. The rate of cup for anteversion and inclination above the safe zone in BP group was significantly larger than that in NP group. There were 4 patients in BP group recording anterior hip dislocation after surgery. Other complications were not observed at last follow-up.

Conclusions: Backward pelvis malrotation may increase TAL guided cup inclination and anteversion, which were inclined to became outlier above the safe zone. This likely increase the rates of dislocation after THA. For the patients with pelvis malrotation, cup positioning should be performed individually instead of guided by TAL.
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http://dx.doi.org/10.1186/s12891-021-04391-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164297PMC
May 2021

Detection of a clinical carbapenem-resistant Citrobacter portucalensis strain and the dissemination of Citrobacter portucalensis in clinical settings.

J Glob Antimicrob Resist 2021 May 25. Epub 2021 May 25.

Shenzhen Institute of Respiratory Diseases, the First Affiliated Hospital (Shenzhen People's Hospital), Southern University of Science and Technology; Second Clinical Medical College (Shenzhen People's Hospital), Jinan University, Shenzhen, China.. Electronic address:

Objective: A clinically isolated carbapenem-resistant Citrobacter portucalensis was characterized by whole genome sequencing (WGS).

Methods: Strain 3839 was identified by Vitek 2.0 and matrix-associated laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Antibiotic susceptibility testing was performed by microbroth dilution method. WGS followed by bioinformatics analysis was conducted.

Results: Strain 3839 was initially identified as C. freundii by Vitek and MALDI-TOF MS, and later was demonstrated as C. portucalensis by WGS analysis. Through average-nucleotide identity analysis, we further detected 55 C. portucalensis genomes which were misidentified as C. freundii in GenBank, and at least 22 were clinical-associated, suggesting that the occurrence of C. portucalensis in the clinical setting might be underestimated by the conventional method. Strain 3839 was extremely drug-resistant, and the presence of multiple resistance determinants was detected by WGS, including bla, bla, bla, bla, qnrB9, qnrA1, aac(6')Ib-cr; aph(6)-Id_1, aph(3'')-Ib, aac(6')Ib-cr, tetA, tet34, and catB3. Forty-five insertion sequences, 8 phages, and 1 integron gene cassette were identified in this strain. The bla gene was carried by an IncX3 plasmid, which was identical to a plasmid detected in a C. freundii strain. The genetic context of bla was IS30-bla-ble-trpF-dsbD-cutA1-groES-groEL-IS91.

Conclusion: To our knowledge, this is the first report of carbapenem-resistant C. portucalensis isolated from clinical samples. The bla gene carried by C. portucalensis may transmit among Citrobacter spp. mediated by plasmids. Together with the underestimation of clinical occurrence caused by the misidentification, our study warrants the necessity of preventing the dissemination of such emerging drug-resistant species in clinical settings.
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http://dx.doi.org/10.1016/j.jgar.2021.04.027DOI Listing
May 2021

An alternatingly amphiphilic, resistance-resistant antimicrobial oligoguanidine with dual mechanisms of action.

Biomaterials 2021 May 17;275:120858. Epub 2021 May 17.

Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chem-/Bio-Sensing and Chemometrics, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, And School of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, 410082, China. Electronic address:

The increasing number of infections caused by multi-drug resistance (MDR) bacteria is an omen of a new global challenge. As one of the countermeasures under development, antimicrobial peptides (AMPs) and AMP mimics have emerged as a new family of antimicrobial agents with high potential, due to their low resistance generation rate and effectiveness against MDR bacterial strains resulted from their membrane-disrupting mechanism of action. However, most reported AMPs and AMP mimics have facially amphiphilic structures, which may lead to undesired self-aggregation and non-specific binding, as well as increased cytotoxicity toward mammalian cells, all of which put significant limits on their applications. Here, we report an oligomer with the size of short AMPs, with both hydrophobic carbon chain and cationic groups placed on its backbone, giving an alternatingly amphiphilic structure that brings better selectivity between mammalian and bacterial cell membranes. In addition, the oligomer shows affinity toward DNA, thus it can utilize bacterial DNA located in the vulnerable nucleoid as the second drug target. Benefiting from these designs, the oligomer shows higher therapeutic index and synergistic effect with other antibiotics, while its low resistance generation rate and effectiveness on multi-drug resistant bacterial strains can be maintained. We demonstrate that this alternatingly amphiphilic, DNA-binding oligomer is not only resistance-resistant, but is also able to selectively eliminate bacteria at the presence of mammalian cells. Importantly, the oligomer exhibits good in vivo activity: it cleans all bacteria on Caenorhabditis elegans without causing apparent toxicity, and significantly improves the survival rate of mice with severely infected wounds in a mice excision wound model study.
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http://dx.doi.org/10.1016/j.biomaterials.2021.120858DOI Listing
May 2021

Long-term results of combined porous tantalum augments and titanium-coated cups for Paprosky type III bone defects in acetabular revision.

Int Orthop 2021 07 21;45(7):1699-1706. Epub 2021 May 21.

Department of Orthopedics, West China Hospital/West China School of Medicine, Sichuan University, 37# Wuhou Guoxue Road, Chengdu, 610041, People's Republic of China.

Purpose: The use of porous tantalum augments and titanium-coated cups in primary total hip arthroplasty (THA) with acetabular defects has shown satisfactory outcomes in our centre. The aim of this study was to report the long-term radiological and clinical outcomes of using this combination for Paprosky type III acetabular bone defects in acetabular revision.

Patients And Methods: Between January 2007 and January 2015, 45 patients with Paprosky type III acetabular defects underwent acetabular revision using a combination of porous tantalum augments and titanium-coated cups. Among these, 41 patients (41 hips) had complete follow-up. Thirty-one patients had a Paprosky type IIIA defect, and ten patients had a Paprosky type IIIB defect. No patients had pelvic discontinuity. There were 20 males and 21 females with an average age of 63.2 years (range 35-80) at the time of revision surgery.

Results: The mean follow-up was 122.8 months (range 69-165). The Harris Hip Score (HHS) improved significantly from 32.1 points (range 17-58) pre-operatively to 85.3 points (63-98) at the last follow-up. The Short Form-12 (SF-12) and Hip Dysfunction and Osteoarthritis Outcome Score (HOOS) improved significantly for each item at the last follow-up. Two patients had a high hip centre post-operatively. One patient had recurrent dislocation but did not require re-revision. No further revision of the acetabular components was required, and all hips remained stable at the last follow-up with bony ingrowth of the acetabular components according to the criteria of Moore et al. CONCLUSION: The combination of tantalum augments and conventional titanium-coated cups achieved satisfactory long-term radiographic and clinical outcomes for Paprosky type III acetabular bone defects without pelvic discontinuity.
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http://dx.doi.org/10.1007/s00264-021-05075-5DOI Listing
July 2021

Sildenafil for pulmonary hypertension in neonates: An updated systematic review and meta-analysis.

Pediatr Pulmonol 2021 Aug 13;56(8):2399-2412. Epub 2021 May 13.

Department of Neonatology and Pediatrics, The First Affiliated Hospital, Jinan University, Guangzhou, China.

Objectives: To provide an updated review and meta-analysis on the efficacy and safety of sildenafil for treating persistent pulmonary hypertension in neonates (PPHN).

Methods: PubMed/Medline, SCOPUS, Cochrane Central Register of Controlled Trials, and Web of Science were searched from the inception of publication to January 2021. The principal outcomes include oxygenation parameters, hemodynamic metrics and echocardiographic measurements, as well as adverse outcomes.

Results: A total of eight studies were included with 216 term and premature neonates with PPHN. Compelling evidence showed the use of sildenafil could improve the prognosis of PPHN neonates, compared with baseline or placebo in neonates with PPHN, and a time-dependent pattern of the improvements can be observed. After 24 h of treatment, the Oxygenation index suggested a steady decrease (SD: -1.80, 95% confidence interval [CI]: -2.92, -0.67) and sildenafil exerted peak effects after 72 h of treatment (SD: -4.02, 95% CI: -5.45, -2.59). No clinically significant side effects were identified. Egger's test and funnel plots of the major outcomes were performed, and the publication bias was not significant.

Conclusion: Improvements were shown in oxygenation index, pulmonary arterial pressure, and adverse outcomes after using sildenafil for PPHN in neonates. However, future research with robust longitudinal or randomized controlled design is still needed.
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http://dx.doi.org/10.1002/ppul.25444DOI Listing
August 2021

[Identification of novel pathogenic variants of TRIOBP gene in a pedigree affected with non-syndromic deafness].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 May;38(5):454-457

Graduate School of Guangxi University of Traditional Chinese Medicine, Nanning, Guangxi 530200, China.

Objective: To explore the genetic basis for a Chinese pedigree affected with non-syndromic hearing loss (NSHL).

Methods: Commercialized gene chip was applied to detect common mutations associated with congenital deafness. Whole exome sequencing was carried out for patients for whom gene chip yielded a negative result. Candidate variants were verified by Sanger sequencing.

Results: Two patients from the pedigree were discovered to carry compound heterozygous variants of the TRIOBP gene, namely c.3299C>A and c.5185-2A>G. Their parents had normal hearing and were both heterozygous carriers of the above variants. Both variants had co-segregated with the disease phenotype in the pedigree and were unreported previously.

Conclusion: Pathogenic variants of the TRIOBP gene comprise an important factor for NSHL. The novel c.5185-2A>G and c.3299C>A variants discovered in this study have enriched the mutational spectrum of the TRIOBP gene and enabled molecular diagnosis and genetic counseling for the family.
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http://dx.doi.org/10.3760/cma.j.cn511374-20200609-00420DOI Listing
May 2021

Effects of low voltage electrostatic field on the microstructural damage and protein structural changes in prepared beef steak during the freezing process.

Meat Sci 2021 Sep 24;179:108527. Epub 2021 Apr 24.

School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China; Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230601, China. Electronic address:

This study investigated the effect of low voltage electrostatic field (LVEF) on the microstructure damage and protein structure changes of prepared beef steak during freezing. The scanning electron microscopy results showed that LVEF-assisted freezing (LVEFF) minimized the gaps in the cross section between muscle fibers induced by freezing and thus improved fiber compactness. Furthermore, LVEFF reduced the length of the enlarged sarcomere, repaired the Z-line fractures, and intensified the dismission of the A band in the air-blast freezing (AF) process. The decreased carbonyl content and increased total sulfhydryl content indicated that LVEFF reduced protein oxidation in the freezing process. In addition, the results of Raman spectroscopy and fluorescence spectroscopy revealed that LVEFF minimized the changes in protein secondary and tertiary structures during freezing. In conclusion, utilization of LVEF in the freezing of prepared beef steak could reduce both the microstructure damage and protein structure changes in the freezing process.
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http://dx.doi.org/10.1016/j.meatsci.2021.108527DOI Listing
September 2021

Elevated LOXL2 expression by LINC01347/miR-328-5p axis contributes to 5-FU chemotherapy resistance of colorectal cancer.

Am J Cancer Res 2021 15;11(4):1572-1585. Epub 2021 Apr 15.

Department of Oncology, 960th Hospital of PLA Jinan 250031, China.

Chemotherapy resistance after curative surgery is a major contributor to the mortality of colorectal cancer (CRC). Detailed mechanism studies of specific molecular alterations are critical to improving the available therapies for long-term disease administration. We explored the functional role of LINC01347 in chemotherapy resistance of CRC. Elevated LINC01347 expression was correlated with CRC disease progression during chemotherapy treatment. However, the functional role of LINC01347 and mechanism remained undefined. In this study, we demonstrated that elevated LINC01347 expression was correlated with late clinical stage and poor prognosis in CRC tumor tissues with TCGA data. Exogenous LINC01347 expression promoted cell proliferation and 5-FU resistance of CRC cells, while LINC01347 knockdown attenuated cell growth and 5-FU resistance in vitro and in vivo. Molecular analysis indicated that LINC01347 participated in the transcriptional regulation of LOXL2 by sponging miR-328-5p. LOXL2 knockdown impaired the LINC01347 overexpression induced 5-FU resistance in CRC cells. The clinical analysis supported miR-328-5p/LOXL2 as a candidate biomarker for chemotherapy resistance of CRC patients. Our study provided a molecular basis for the development of 5-FU based chemotherapy resistance in CRC by LINC01347/miR-328/LOXL2 axis. We identified LINC01347 as a prognostic biomarker and potential therapeutic target against 5-FU based chemotherapy resistance of CRC.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085882PMC
April 2021

Magnetic recyclable heterogeneous catalyst FeO/g-CN for tetracycline hydrochloride degradation via photo-Fenton process under visible light.

Environ Technol 2021 May 8:1-14. Epub 2021 May 8.

Key Laboratory of Nanominerals and Pollution Control of Higher Education Institutes, School of Resources and Environmental Engineering, Hefei University of Technology, Hefei, People's Republic of China.

Antibiotic pollution of water resources is a global problem, and the development of new treatments for destroying antibiotics in water is a priority research. We successfully manufactured recyclable magnetic FeO/g-CN through the electrostatic self-assembly method. Selecting tetracycline (TC) as the target pollutant, using FeO/g-CN and HO developed a heterogeneous optical Fenton system to remove TC under visible light. FeO/g-CN was systematically characterized by SEM, TEM, XRD, FTIR, XPS, DRS, and electrochemical methods. The removal efficiency of 7% FeO/g-CN at pH = 3, HO = 5 mM, and catalyst dosage of 1.0 g/L can reach 99.8%. After magnetic separation, the FeO/g-CN photocatalyst can be recycled five times with minimal efficiency loss. The excellent degradation performance of the prepared catalyst may be attributed to the proper coupling interface between FeO and g-CN which promotes the separation and transfer of photogenerated electrons. Photogenerated electrons can also accelerate the conversion of Fe to Fe, thereby producing more ˙OH. The new FeO/g-CN can be used as a raw material for advanced oxidation of water contaminated by refractory antibiotics.
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http://dx.doi.org/10.1080/09593330.2021.1921052DOI Listing
May 2021

Adsorption behaviours and transfer simulation of levofloxacin in silty clay.

Environ Sci Pollut Res Int 2021 Apr 16. Epub 2021 Apr 16.

Institute of Hydrogeology and Environmental Geology, Chinese Academy of Geological Sciences, Shijiazhuang, 050000, Hebei Province, China.

Fluoroquinolone antibiotics in soil can cause serious antibiotic pollution. Adsorption is the main factor that influences their destination and transport of antibiotics. Therefore, research on the behaviour of antibiotics once they reach the soil environment is meaningful to design appropriate measures to reduce their potential risks. This research took levofloxacin (LVFX) as the research object and used a static adsorption experiment to study the adsorption behaviour of the vadose zone of silty clay on the North China Plain. The results showed that LVFX had high retention in silty clay, with an average adsorption ratio of more than 90%. Adsorption of LVFX on silty clay reached equilibrium in 24 h with an adsorption amount of 93.5 mg/kg at an initial LVFX concentration of 10 mg/L. Acidity, cations and soil organic matter could affect the adsorption of LVFX, with adsorption variation ratio of 3.3%, 3.4% and 0.6%, respectively. In addition, numerical simulation with Hydrus-1D was utilized, and the results show that LVFX may infiltrate into underground water through silty clay after 28 days and completely penetrate in 100 days.
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http://dx.doi.org/10.1007/s11356-021-13955-xDOI Listing
April 2021

Inhibition of syndecan-4 reduces cartilage degradation in murine models of osteoarthritis through the downregulation of HIF-2α by miR-96-5p.

Lab Invest 2021 Aug 13;101(8):1060-1070. Epub 2021 Apr 13.

Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, PR China.

The membranous receptor syndecan-4 (SDC-4) and the nuclear transcription factor hypoxia-induced factor-2α (HIF-2α) play critical roles in the pathogenesis of osteoarthritis (OA). The aim of this study was to determine whether SDC-4 inhibition downregulates HIF-2a expression by microRNA-96-5p (miR-96-5p) in murine chondrocyte and cartilage tissue. The OA model was induced surgically in mice, and SDC-4 polyclonal antibody, HIF-2α small interfering RNA (siRNA) and its control, miR-96-5p mimics and its scrambled controls or anti-miR-96-5p and its control were then injected into the knee joints. At 2 and 4 weeks after surgery, OA progression was evaluated microscopically, histologically, radiographically and immunohistochemically in these mice. Real-time polymerase chain reaction (RT-PCR) and western blotting were performed after treating with antibody and transfecting with miRNA mimic or siRNA to determine their effects on OA-related mediators. The potential miRNAs related to OA development were identified by using miRNA microarray analysis. Whether miRNAs play a pivotal role in OA development in vivo or in vitro was also investigated. MiR-96-5p expression was upregulated by SDC-4-specific antibodies in chondrocytes and cartilage tissue, and miR-96-5p directly targeted the 3'-UTR of HIF-2α to inhibit HIF-2α signaling in murine chondrocytes. Moreover, we demonstrated that anti-SDC-4-attenuated IL-1β-induced chondrocyte hypertrophy and cartilage degradation by inhibiting HIF-2α signaling by a miR-96-5p-dependent mechanism. Our study revealed that the inhibition of SDC-4 exerts its effects on both cartilage homeostasis and the chondrocyte hypertrophy phenotype by inducing miR-96-5p expression, which results in targeting HIF-2α 3'-UTR sequences and inhibiting HIF-2α in murine cartilage tissue and chondrocytes.
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http://dx.doi.org/10.1038/s41374-021-00595-5DOI Listing
August 2021

Identification and Pyramiding of QTLs for Rice Grain Size Based on Short-Wide Grain CSSL-Z563 and Fine-Mapping of qGL3-2.

Rice (N Y) 2021 Apr 13;14(1):35. Epub 2021 Apr 13.

Rice Research Institute, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.

Background: Chromosome segment substitution lines (CSSLs) can be used to dissect complex traits, from which single-segment substitution lines (SSSLs) containing a target quantitative trait loci (QTL) can be developed, and they are thus important for functional analysis and molecular breeding.

Results: A rice line with short wide grains, CSSL-Z563, was isolated from advanced-generation backcross population (BCF) derived from 'Xihui 18' (the recipient parent) and 'Huhan 3' (the donor parent). Z563 carried seven segments from 'Huhan 3', distributed on chromosomes 3, 7, and 8, with average substitution length of 5.52 Mb. Eleven QTLs for grain size were identified using secondary F population of 'Xihui 18'/Z563. The QTLs qGL3-1, qGL3-2, and qGL7 control grain length in Z563 and have additive effects to reduce grain length; qGW3-1 and qGW3-2 control grain width in Z563 and have additive effects to increase grain width. Four SSSLs, three double-segment substitution lines (D1-D3), and two triple-segment substitution lines (T1 and T2) were developed containing the target QTLs. The genetic stability of eight QTLs, including qGL3-2, qGL3-1, and qGL7, was verified by the SSSLs. D1 (containing qGL3-2 and qGL3-1), D2 (qGL3-1 and qGL7), and T1 (qGL3-2, qGL3-1, and qGL7) had positive epistatic effects on grain length, and their grain length was shorter than that of the corresponding SSSLs. The QTL qGL3-2 was fine-mapped to a 696 Kb region of chromosome 3 containing five candidate genes that differed between 'Xihui 18' and Z563. These results are important for functional research on qGL3-2 and molecular breeding of hybrid rice cultivars.

Conclusions: The short and wide grain of Z563 was mainly controlled by qGL3-1, qGL3-2, qGL7, qGW3-1 and qGW3-2. The major QTL qGL3-2 was fine-mapped to a 696 Kb region of chromosome 3 containing five candidate genes. Different QTLs pyramiding displayed various phenotypes. In essence, the performance after pyramiding of genes depended on the comparison between the algebraic sum of the additive and epistatic effects of QTLs in the pyramidal line and the additive effect value of the single QTL. The results lay good foundation in the functional analysis of qGL3-2 and molecular design breeding of novel hybrid rice cultivars.
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http://dx.doi.org/10.1186/s12284-021-00477-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044274PMC
April 2021

Increased sensitivity to TNF-α promotes keloid fibroblast hyperproliferation by activating the NF-κB, JNK and p38 MAPK pathways.

Exp Ther Med 2021 May 17;21(5):502. Epub 2021 Mar 17.

Department of Plastic and Burn Surgery, West China School of Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.

Hyperproliferation of fibroblasts is the main cause of keloid formation. However, the pathogenesis of keloids has yet to be fully elucidated. Tumor necrosis factor (TNF)-α may play an important role in the formation and proliferation of keloids, as it is implicated in the pathogenesis of various fibrous disorders. In the present study, the expression level of TNF-α and its receptors, soluble TNF receptor (sTNFR)1 and sTNFR2, in the peripheral blood and skin tissues was detected by ELISA, reverse transcription-quantitative PCR or immunohistochemistry. There was no statistically significant difference in the expression of TNF-α and sTNFR2 in the peripheral blood and skin tissues between patients with keloids and healthy participants (P>0.05), while the sTNFR1 mRNA level in fibroblasts cultured and its protein level in keloid skin samples were significantly higher compared with those in normal skin (P<0.05). Subsequently, TNF-α recombinant protein was used to treat keloid-derived and normal skin fibroblasts, and it was observed that TNF-α promoted the proliferation of keloid fibroblasts (KFs), but had little effect on normal skin fibroblasts. Furthermore, it was observed that TNF-α stimulation led to the activation of the nuclear factor (NF)-κB, c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MAPK) pathways in KFs. In conclusion, KFs exhibited increased expression of sTNFR1, which may contribute to the increased sensitivity to TNF-α, resulting in low concentrations of TNF-α activating the NF-κB, JNK and p38 MAPK pathways, thereby promoting the sustained and excessive proliferation of KFs.
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http://dx.doi.org/10.3892/etm.2021.9933DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005672PMC
May 2021