Publications by authors named "Kai Zhou"

530 Publications

Corrigendum: Comparative respiratory tract microbiome between carbapenem-resistant colonization and ventilator associated pneumonia.

Front Microbiol 2022 20;13:985295. Epub 2022 Jul 20.

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

[This corrects the article DOI: 10.3389/fmicb.2022.782210.].
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http://dx.doi.org/10.3389/fmicb.2022.985295DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355586PMC
July 2022

Hierarchical Heterostructure Engineering of Layered Double Hydroxides on Nickel Sulfides Heteronanowire Arrays as Efficient Cathode for Alkaline Aqueous Zinc Batteries.

Small 2022 Jul 30:e2202799. Epub 2022 Jul 30.

Guangzhou Key Laboratory of Sensing Materials & Devices, Center for Advanced Analytical Science, c/ o School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou, 510006, P. R. China.

Aqueous alkaline rechargeable nickel-zinc (Ni-Zn) batteries possess great potential for large-scale energy storage systems because of their high output voltage, cheap cost, and intrinsic safety. However, the practical applicability of Ni-Zn batteries has been limited by traditional Ni-based cathodes with low capacity and poor cycle stability. Rational design of electrode structure and composition is highly desired but still significantly challenging. Herein, uniform self-supported hierarchical heterostructure composites interacting NiCo-layered double hydroxide with 1D nickel sulfides heteronanowire rooted on Ni foam (NF\Ni S /[email protected]) are successfully developed by a hydrothermal sulfurization-electrodeposition process. The self-supported 3D hierarchical heterostructured composites nanoarray provides abundant reactive sites, rapid ion diffusion channels, and fast electron transfer routes, as well as strong structural stability. More significantly, the strong interfacial charge transfer between Ni S /NiS heteronanowire and NiCo-LDH effectively modifies the electronic structure of the composites and thereby improving the reaction kinetics. Consequently, the NF\Ni S /[email protected] electrode presents a superior capacity of 434.5 mAh g (1.73 mAh cm ) at 3 mA cm . In addition, the fabricated NF\Ni S /[email protected]//Zn battery can offer a maximal energy density and power density as large as 556.3 Wh kg and 26.3 kW kg , respectively, as well as an exceptional cycling performance.
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http://dx.doi.org/10.1002/smll.202202799DOI Listing
July 2022

Quality of Life and Mental Health Status in Recovered COVID-19 Subjects at Two Years after Infection in Taizhou, China: A Longitudinal Cohort Study.

Brain Sci 2022 Jul 18;12(7). Epub 2022 Jul 18.

Department of Clinical Laboratory, Taizhou Hospital, Zhejiang University, Linhai 317000, China.

According to previous studies, mental status in 1-year COVID-19 survivors might range from 6-43%. Longer-term psychological consequences in recovered COVID-19 subjects are unknown, so we analyzed longer-term quality of life and mental status in recovered COVID-19 subjects at 2 years after infection. Among 144 recovered COVID-19 subjects in the Taizhou region, 73 and 45 completed face-to-face follow-ups at the first year and second year after infection, respectively, with a 61.7% follow-up rate. The questionnaire, which was administered at both follow-ups, included questions about quality of life, psychological health, and post-traumatic stress disorder (PTSD). The Mann-Whitney U test was used to the differences of each scale between the first and second year. Among the 45 people who completed both follow-up visits, the incidence of psychological problems was 4.4% (2/45) in the first year, and no new psychological abnormalities were observed in the second year. Quality of life improved, while the General Health Questionnaire (GHQ-12) and Impact of Event Scale-Revised (IES-R) scores did not improve over time. The incidence of mental disorders was lower than those in previous studies. Multidisciplinary management for COVID-19 in this study hospital may have reduced the frequency to a certain extent. However, among those with mental health problems, such problems may exist for a long time, and long-term attention should be given to the psychological status of recovered COVID-19 subjects.
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http://dx.doi.org/10.3390/brainsci12070939DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316455PMC
July 2022

Effect of Xuanwei Ham Proteins with Different Ripening Periods on Lipid Metabolism, Oxidative Stress, and Gut Microbiota in Mice.

Mol Nutr Food Res 2022 Jul 25:e2101020. Epub 2022 Jul 25.

School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230601, China.

Scope: Consumption of dry-cured ham may have a cardiovascular disease preventive capacity. However, whether ham protein in the maintenance of health and prevention of disease is dependent on ripening periods need to be clarified.

Methods And Results: Fifty C57BL/6 mice consume a diet that contains either Casein, raw ham protein (XWH0), or Xuanwei ham protein after 1-, 2-, or 3-years of maturation (XWH1, XWH2, or XWH3) for 4 weeks. Results show that longer ripening periods in the Xuanwei ham groups are negatively correlated with body weight gain, adipose tissue weight, total cholesterol (TC), and triglycerides levels in serum and liver, and these proteins intake could maintain liver health, reduce lipid deposition and oxidative stress, regulate lipid metabolism-related genes expression, alter the gut microbiota, and promote short-chain fatty acid production. Remarkably, XWH3 intake maintains lower TC and low-density lipoprotein cholesterol levels in serum and has high antioxidative activity as well. This regulation of lipid metabolism and oxidative stress may be related to the AMPK/Nrf2 signaling.

Conclusion: Xuanwei ham dietary proteins that have endured a specific ripening period may have preventive effects on metabolic syndrome, which provide a basis for new ideas for nutritional health interventions.
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http://dx.doi.org/10.1002/mnfr.202101020DOI Listing
July 2022

Coordination of zygotic genome activation entry and exit by H3K4me3 and H3K27me3 in porcine early embryos.

Genome Res 2022 Jul 22. Epub 2022 Jul 22.

Huazhong Agricultural University

Histone modifications are critical epigenetic indicators of chromatin state associated with gene expression. Although the reprogramming patterns of H3K4me3 and H3K27me3 have been elucidated in mouse and human preimplantation embryos, the relationship between these marks and zygotic genome activation (ZGA) remains poorly understood. By ultra-low-input native chromatin immunoprecipitation and sequencing, we profiled global H3K4me3 and H3K27me3 in porcine oocytes and in vitro fertilized (IVF) embryos. We found that promoters of ZGA genes occupied sharp H3K4me3 peaks in oocytes, and these peaks became broader after fertilization, and reshaped into sharp again during ZGA. By simultaneous depletion of H3K4me3 demethylase KDM5B and KDM5C, we determined that broad H3K4me3 domain maintenance impaired ZGA gene expression, suggesting its function to prevent premature ZGA entry. By contrast, broad H3K27me3 domains underwent global removal upon fertilization, followed by a re-establishment for H3K4me3/H3K27me3 bivalency in morulae. We also found that bivalent marks were deposited at promoters of ZGA genes, and inhibiting this deposition was correlated with the activation of ZGA genes. It suggests that promoter bivalency contributes to ZGA exit in porcine embryos. Moreover, we demonstrated that aberrant reprogramming of H3K4me3 and H3K27me3 triggered ZGA dysregulation in somatic cell nuclear transfer (SCNT) embryos, whereas H3K27me3-mediated imprinting did not exist in porcine IVF and SCNT embryos. Our findings highlight two previously unknown epigenetic reprogramming modes coordinated with ZGA in porcine preimplantation embryos. Finally, the similarities observed between porcine and human histone modification dynamics suggest that the porcine embryo may also be a useful model for human embryo research.
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http://dx.doi.org/10.1101/gr.276207.121DOI Listing
July 2022

Frequent convergence of and carbapenemase genes in complex driven by epidemic plasmids and host incompatibility.

Emerg Microbes Infect 2022 Dec;11(1):1959-1972

Shenzhen Institute of Respiratory Diseases, Second Clinical Medical College (Shenzhen People's Hospital), Jinan University; the First Affiliated Hospital (Shenzhen People's Hospital), Southern University of Science and Technology, Shenzhen, People's Republic of China.

Convergence of and carbapenemase genes has been sporadically detected in complex (ECC) with an upward trend. However, the state of the epidemic and underlying mechanism of such convergence has been poorly understood. In this study, the co-occurrence of MCR and carbapenemases was systematically analyzed in 230 clinical ECC isolates collected between 2000 and 2018 together with a global dataset consisting of 3,559 ECC genomes compiled from GenBank. We identified 48 /-positive isolates (MCR-ECC) (20.9%) in our collection, and a comparable ratio of MCR-ECC (720/3559, 20.2%) was detected in the global dataset. A high prevalence of carbapenemase-producing MCR-ECC (MCR-CREC) was further identified in the MCR-ECC of both datasets (16/48, 33.3%; 388/720, 53.9%), demonstrating a frequent convergence of and carbapenemase genes in ECC worldwide. An epidemic IncHI2/2A plasmid with a highly conserved backbone was identified and largely contributed to the dissemination of in ECC worldwide. A highly conserved IncX3-type NDM-1-carrying plasmid and IncN-type IMP-4-carrying plasmid were additionally detected in MCR-CREC isolated in China. Our surveillance data showed that MCR-CREC emerged (in 2013) much later than MCR-ECC (in 2000), indicating that MCR-CREC could be derived from MCR-ECC by additional captures of carbapenemase-encoding plasmids. Tests of plasmid stability and incompatibility showed that the -encoding plasmids with the NDM-1-encoding plasmids stably remained in ECC but incompatible in , suggesting that the convergence was host-dependent. The findings extend our concern on the convergence of resistance to the last resort antibiotics and highlight the necessity of continued surveillance in the future.
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http://dx.doi.org/10.1080/22221751.2022.2103456DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359198PMC
December 2022

Osteoarthritis and APOE4 Interact to Influence Memory Decline in Non-Demented Older People.

Authors:
Kai Zhou Peng Wu

J Alzheimers Dis 2022 Jul 15. Epub 2022 Jul 15.

Department of Orthopaedics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Background: The question that whether the presence of osteoarthritis (OA) can modify the effects of apolipoprotein E4 (APOE4) genotype on longitudinal change in cognitive performance among non-demented older people remains unclear.

Objective: To examine whether the association of APOE4 genotype with change in verbal episodic memory over time is modified by the presence of OA among non-demented older people.

Methods: Longitudinal data from 1,400 non-demented older people were obtained from the Alzheimer's Disease Neuroimaging Initiative database. The sample included 466 healthy individuals and 934 mild cognitive impairment. The effects of the OA×APOE4 genotype interaction term on longitudinal change in cognitive performance were examined using linear mixed-effects regression models. Global cognition was assessed by the Mini-Mental State Examination score and Clinical Dementia Rating-Sum of Boxes. Verbal episodic memory was evaluated by the Rey Auditory Verbal Learning Test (RAVLT) immediate recall and delayed recall score.

Results: We found that OA interacted with APOE4 genotype to influence longitudinal change in verbal episodic memory (as assessed by RAVLT immediate recall score) but not global cognition. Specifically, the OA-/APOE4+ group had a steeper decline in RAVLT immediate recall score compared with the OA+/APOE4+ group. However, there was no difference in RAVLT immediate recall score between OA-/APOE4-and OA+/APOE4-individuals.

Conclusion: Our study suggested that the association of APOE4 genotype with change in RAVLT immediate recall score over time is modified by the presence of OA at earliest stages of the disease.
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http://dx.doi.org/10.3233/JAD-220138DOI Listing
July 2022

The evaluation of the immune status of COVID-19 recovered subjects with persistent abnormal lung CT after one year: A longitudinal cohort study.

Int Immunopharmacol 2022 Jul 6;110:109019. Epub 2022 Jul 6.

Department of Clinical Laboratory, Taizhou Hospital of Zhejiang Province affiliated to Wenzhou Medical University, Linhai, Zhejiang, China. Electronic address:

Objectives: COVID-19 is an immune-related disease caused by novel Coronavirus SARS-COV-2. Lung lesions persist in some recovered patients, making long-term follow-up monitoring of their health necessary. The mechanism of these abnormalities is still unclear. In this study, the immune status was observed to explore the immune mechanism of persistent lung CT abnormalities in one-year COVID-19 recovered subjects.

Methods: One-year follow-up of 73 recovered patients from COVID-19 confirmed in Taizhou City, Zhejiang Province, was conducted to collect laboratory indicators such as blood immune cells, cytokines, complement series, immunoglobulin, and lung imaging; According to the results of lung CT, 60 patients were divided into normal CT group (n = 40) and abnormal CT group (n = 20). We compared the dynamic changes of immune indexes at three timepoints namely onset (T1), discharge (T2), and 1-year follow-up (T3), and studied the relationship between immune indexes and pulmonary sequelae.

Results: Compared with the healthy control, there was no significant difference in immune-related indexes, and immune levels had recovered. Patients with elder age, high BMI, severe patients, and those with underlying diseases (hypertension or diabetes) had a higher CT abnormal rate after recovery. Longitudinal observation showed that immunoglobulin increased first and then decreased, immune cell TBNK decreased in the onset period and increased in the recovery period, cytokine level increased significantly in the onset period and decreased to the normal level in the recovery period, and complement series C1q, C3 and C4 increased at the onset and decreased during the one-year follow-up. Complement C3 remained at a high level in the CT abnormal group (CT normal group vs CT abnormal group; P = 0.036). Correlation analysis showed that C3 negatively correlated restrictive ventilation index (TLC-He (ratio) (r = -0.302, P = 0.017). The above results suggest that complement C3 is a negative factor correlating abnormal pulmonary function 1 year after the recovery.

Conclusion: After one year recovering from COVID-19, the subjects were with stable immune indicators. High levels of complement C3 were associated with persistent lung abnormalities in COVID-19 recovered subjects.
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http://dx.doi.org/10.1016/j.intimp.2022.109019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9257193PMC
July 2022

Epidemiology and Genomic Characteristics of Bloodstream Infection Caused by Carbapenem-Resistant With Decreased Susceptibility to Aztreonam/Avibactam in China.

Front Cell Infect Microbiol 2022 22;12:926209. Epub 2022 Jun 22.

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Aztreonam/avibactam (AZA), as one of the novel β-lactamases and β-lactamase inhibitor combinations, is considered to be a promising option for bloodstream infection (BSI) of carbapenem-resistant (CR-Kp). However, decreased susceptibility of AZA activity in has been reported. The aim of this study was to identify the mechanisms of BSI CR-Kp with decreased susceptibility of AZA (minimal inhibitory concentration above 16/4 mg/L) (AZAH-Kp). Nine BSI AZAH-Kp isolates were screened from 317 CR-Kp isolates in Blood Bacterial Resistant Investigation Collaborative System (BRICS) program. Whole genome sequencing, bioinformatics analysis, and the relative expression of , , and were explored for CR-Kp with decreased susceptibility to AZA. The results revealed that elevated inhibitory concentration of AZA has emerged in CR-Kp before previous clinical exposure. In addition, decreased AZA susceptibility was associated with higher KPC expression and changes in OmpK35-37.
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http://dx.doi.org/10.3389/fcimb.2022.926209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9257070PMC
July 2022

Analysis of the Turnover Tendency of College Teachers From the Perspective of Psychology.

Authors:
Ying Zhao Kai Zhou

Front Psychol 2022 21;13:771324. Epub 2022 Jun 21.

School of Cyber Security, Changchun University, Changchun, China.

University teachers are the core of university teaching and scientific research construction and an important link of double first-class construction. With the increasingly fierce academic competition among colleges and universities, the resignation behavior of college teachers is increasing, which has brought great impact on the construction of talents and discipline development in colleges and universities. The survey found that the departure of college teachers is not an isolated phenomenon, but a time-consuming process. Although the turnover tendency of college teachers has not evolved into resignation behavior in most cases, the resignation tendency of college teachers is always accompanied by negative psychology, complaints, and perfunctory work. These bad phenomena have brought double negative effects to colleges and universities and teachers themselves. According to this, psychological analysis of the turnover tendency of college teachers is conducive to building a benign flow mechanism for college teachers, thus supporting the long-term development of colleges and universities.
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http://dx.doi.org/10.3389/fpsyg.2022.771324DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9255425PMC
June 2022

[Research progress of lung aging in chronic respiratory diseases].

Sheng Li Xue Bao 2022 Jun;74(3):479-488

Department of Physiology, School of Basic Medical Science, Central South University, Changsha 410078, China.

Cell aging is an extremely complex process, which is characterized by mitochondrial structural dysfunction, telomere shortening, inflammatory microenvironment, protein homeostasis imbalance, epigenetic changes, abnormal DNA damage and repair, etc. Aging is usually accompanied by structural and functional damage of tissues and organs which further induces the occurrence and development of aging-related diseases. Aging includes physiological aging caused by increased age and pathological aging induced by a variety of factors. Noteworthy, as a target organ directly contacting with the outside air, lung is more prone to various stimuli, causing pathological premature aging which is lung aging. Studies have found that there is a certain proportion of senescent cells in the lungs of most chronic respiratory diseases. However, the underlying mechanism by which these senescent cells induce lung senescence and their role in chronic respiratory diseases is still obscure. This paper focuses on the causes and classification of lung aging, the internal mechanism of lung aging involved in chronic respiratory diseases, and the application of anti-aging treatments in chronic respiratory diseases. We hope to provide new research ideas and theoretical basis for the clinical prevention and treatment in chronic respiratory diseases.
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June 2022

Tuftelin 1 Facilitates Hepatocellular Carcinoma Progression through Regulation of Lipogenesis and Focal Adhesion Maturation.

J Immunol Res 2022 19;2022:1590717. Epub 2022 Jun 19.

State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200240, China.

Hepatocellular carcinoma (HCC) is the most common type of primary liver malignancy with poor prognosis worldwide. Emerging evidences demonstrated critical roles of lipid de novo synthesis in HCC progression, yet its regulatory mechanisms are not fully understood. Herein, we found that tuftelin 1 (TUFT1), an acidic phosphorylated glycoprotein with secretory capacity, was significantly upregulated in HCC and had an excellent correlation with patient survival and malignancy features. Through database mining and experimental validation, we found that TUFT1 was associated with fatty acid metabolism and promoted lipid accumulation in HCC cells. Further, we found that TUFT1 can interact with CREB1, a transcription factor for hepatic lipid metabolism, and regulate its activity and the transcriptions of key enzymes for lipogenesis. TUFT1 promoted HCC cell proliferation significantly, which was partially reversed by treatment of an inhibitor of CREB1, KG-501. Moreover, TUFT1 promoted the capacity of HCC cell invasion in vitro, which was likely mediated by its association with zyxin, a zinc-binding phosphoprotein responsible for the formation of fully mature focal adhesions on extracellular matrix. We found that TUFT1 can interact with ZYX and inhibit its expression and recruitments to focal complexes in HCC cells. Collectively, our study uncovered new regulatory mechanisms of TUFT1-mediated lipogenesis, cell proliferation, and invasion.
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http://dx.doi.org/10.1155/2022/1590717DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234046PMC
July 2022

Tumor-targeted/reduction-triggered composite multifunctional nanoparticles for breast cancer chemo-photothermal combinational therapy.

Acta Pharm Sin B 2022 Jun 10;12(6):2710-2730. Epub 2021 Sep 10.

State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Collaborative Innovation Center of Biotherapy, Chengdu 610041, China.

Breast cancer has become the most commonly diagnosed cancer type in the world. A combination of chemotherapy and photothermal therapy (PTT) has emerged as a promising strategy for breast cancer therapy. However, the intricacy of precise delivery and the ability to initiate drug release in specific tumor sites remains a challenging puzzle. Therefore, to ensure that the therapeutic agents are synchronously delivered to the tumor site for their synergistic effect, a multifunctional nanoparticle system (PCRHNs) is developed, which is grafted onto the prussian blue nanoparticles (PB NPs) by reduction-responsive camptothecin (CPT) prodrug copolymer, and then modified with tumor-targeting peptide cyclo(Asp-d-Phe-Lys-Arg-Gly) (cRGD) and hyaluronic acid (HA). PCRHNs exhibited nano-sized structure with good monodispersity, high load efficiency of CPT, triggered CPT release in response to reduction environment, and excellent photothermal conversion under laser irradiation. Furthermore, PCRHNs can act as a photoacoustic imaging contrast agent-guided PTT. studies indicate that PCRHNs exhibited excellent biocompatibility, prolonged blood circulation, enhanced tumor accumulation, allow tumor-specific chemo-photothermal therapy to achieve synergistic antitumor effects with reduced systemic toxicity. Moreover, hyperthermia-induced upregulation of heat shock protein 70 in the tumor cells could be inhibited by CPT. Collectively, PCRHNs may be a promising therapeutic way for breast cancer therapy.
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http://dx.doi.org/10.1016/j.apsb.2021.08.021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214336PMC
June 2022

Integrins are double-edged swords in pulmonary infectious diseases.

Biomed Pharmacother 2022 Jun 18;153:113300. Epub 2022 Jun 18.

Department of Respiratory Medicine, National Clinical Research Center for Respiratory Diseases, Xiangya Hospital, Central South University, Changsha, Hunan, China; Department of Physiology, School of Basic Medicine Science, Central South University, Changsha, Hunan, China; Research Center of China-Africa Infectious Diseases, Xiangya School of Medicine Central South University, Changsha, Hunan, China. Electronic address:

Integrins are an important family of adhesion molecules that are widely distributed on immune cells in the lungs. Of note, accumulating evidences have shown that integrins are double-edged swords in pulmonary infectious diseases. On one hand, integrins promote the migration of immune cells to remove the invaded pathogens in the infected lungs. However, on the other hand, integrins also act as the targets for pathogens to escape from host immune system, which is a potential factor leading to further tissue damage. Thus, the innovative therapeutic strategies based on integrins has inspired well-founded hopes to treat pulmonary infectious diseases. In this review, we illustrate the involvement of integrins in pulmonary infectious diseases, and further discuss the innovative therapeutic targets based on integrins.
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http://dx.doi.org/10.1016/j.biopha.2022.113300DOI Listing
June 2022

Hierarchical Accumulation of Histone Variant H2A.Z Regulates Transcriptional States and Histone Modifications in Early Mammalian Embryos.

Adv Sci (Weinh) 2022 Jun 19:e2200057. Epub 2022 Jun 19.

Institute of Stem Cell and Regenerative Biology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, P. R. China.

Early embryos undergo extensive epigenetic reprogramming to achieve gamete-to-embryo transition, which involves the loading and removal of histone variant H2A.Z on chromatin. However, how does H2A.Z regulate gene expression and histone modifications during preimplantation development remains unrevealed. Here, by using ultra-low-input native chromatin immunoprecipitation and sequencing, the genome-wide distribution of H2A.Z is delineated in mouse oocytes and early embryos. These landscapes indicate that paternal H2A.Z is removed upon fertilization, followed by unbiased accumulation on parental genomes during zygotic genome activation (ZGA). Remarkably, H2A.Z exhibits hierarchical accumulation as different peak types at promoters: promoters with double H2A.Z peaks are colocalized with H3K4me3 and indicate transcriptional activation; promoters with a single H2A.Z peak are more likely to occupy bivalent marks (H3K4me3+H3K27me3) and indicate development gene suppression; promoters with no H2A.Z accumulation exhibit persisting gene silencing in early embryos. Moreover, H2A.Z depletion changes the enrichment of histone modifications and RNA polymerase II binding at promoters, resulting in abnormal gene expression and developmental arrest during lineage commitment. Furthermore, similar transcription and accumulation patterns between mouse and porcine embryos indicate that a dual role of H2A.Z in regulating the epigenome required for proper gene expression is conserved during mammalian preimplantation development.
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http://dx.doi.org/10.1002/advs.202200057DOI Listing
June 2022

A RAC-alpha serine/threonine-protein kinase (CgAKT1) involved in the synthesis of CgIFNLP in oyster Crassostrea gigas.

Fish Shellfish Immunol 2022 Jun 14;127:129-139. Epub 2022 Jun 14.

Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Functional Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266235, China; Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China. Electronic address:

The RAC-alpha serine/threonine-protein kinase (AKT) is one of the most important protein kinases involved in many biological processes in eukaryotes. In the present study, a novel AKT homologue named CgAKT1 was identified from the Pacific oyster Crassostrea gigas. The open reading frame (ORF) of CgAKT1 cDNA was of 1482 bp encoding a peptide with 493 amino acid residues. There were classical domains in the predicted CgAKT1 protein, including an N-terminal pleckstrin homology domain, a central catalytic domain and a C-terminal hydrophobic domain. The mRNA transcripts of CgAKT1 were detected in all the examined tissues of C. gigas with higher level in gills (8.24-fold of that in mantle, p < 0.05) and haemocytes (3.62-fold of that in mantle, p < 0.05). After poly (I:C) stimulation, the mRNA expression of CgAKT1 decreased significantly in haemocytes from 3 h (0.44-fold of that in the control group, p < 0.001) to 24 h (0.20-fold of that in the control group, p < 0.001), and then increased significantly at 48 h (3.65-fold of that in the control group, p < 0.05). The expression level of CgAKT1 mRNA increased significantly at 6 h after rCgIFNLP stimulation, which was 3.60-fold of that in the control group (p < 0.001). The Alexa Fluor 488 positive signals of CgAKT1 protein were found to be distributed in the cytoplasm and cell membrane of haemocytes, while those in the cytoplasm became weaker after poly (I:C) stimulation. In CgAKT1-RNAi oysters, the mRNA expression of cyclic GMP-AMP synthase (CgcGAS) and TANK-binding kinase 1 (CgTBK1) did not change significantly, but the mRNA expression level of stimulator of interferon gene (CgSTING), interferon regulatory factor-1 (CgIRF-1), interferon regulatory factor-8 (CgIRF-8) and IFN-like protein (CgIFNLP) increased significantly, which was 1.40-fold, 1.53-fold, 1.72-fold and 1.99-fold of that in EGFP-RNAi oysters (p < 0.05), respectively. In CgIFNLP-RNAi oysters, the transcripts of CgAKT1 decreased significantly compared to those in EGFP-RNAi oysters (0.16-fold, p < 0.01). Moreover, the expression of p-CgTBK1, CgSTING and CgIFNLP at the protein level in the oysters treated with p-AKT1 activator (SC-79) was significantly suppressed after poly (I:C) stimulation. After the transfection of CgAKT1, the expression of p-cGAS protein in HEK293T cells increased significantly, while the cyclic GMP-AMP in the cells and the interferon (IFN-β) in the cell culture fluid decreased significantly compared with that in the control group. These results indicated that CgAKT1 might play a negative role in antiviral immunity of oyster by regulating the synthesis of CgIFNLP.
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http://dx.doi.org/10.1016/j.fsi.2022.05.057DOI Listing
June 2022

Method for Evaluating Fault Hydraulic Conductive Property and Its Application in Shandong, China.

ACS Omega 2022 Jun 25;7(22):18292-18305. Epub 2022 May 25.

School of Resources and Geosciences, China University of Mining and Technology, Xuzhou 221116, Jiangsu, China.

Coal remains the largest contributor to the energy structure of China. However, coal production is frequently threatened by groundwater inrush accidents caused by hydraulically conductive faults. Despite the threat of such accidents, research on methods for evaluating fault hydraulic conductive property without hydraulic tests has seldom been conducted. Many faults exist in coal mines in Shandong, China. However, due to economic and technical limitations, hydrological tests are rarely performed and can be performed on only a few faults. The hydraulic conductive property of many faults is unknown, which has prevented serious groundwater inrush accidents and casualties from being avoided. Using accessible geological exploration data, we propose a method for evaluating fault hydraulic conductive property in the Jining coalfield, Shandong, China. Mudstone smearing, lithologic contact relations on the fault plane, geostress, water pressure, plastic deformation of mudstone, and the argillaceous content of the fault zone were selected as factors, and six quantitative indicators were proposed: the shale gouge ratio (SGR), lithologic juxtaposition diagram (LJD), fault closure coefficient (FCC), water pressure coefficient (WPC), mudstone deformation coefficient (MDC), and shale smear factor (SSF). The fuzzy analytic hierarchy process (FAHP) was applied to calculate the weights and establish lateral and vertical hydraulic conductive property (L and V) evaluation models for faults. The fault hydraulic conductivities were then classified as weak, medium, or strong. The hydrochemical experiments and the limited number of exposed faults were used for validation. Hence, the evaluation models were considered effective at determining the hydraulic conductive property of faults in the Jining coalfield, China.
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http://dx.doi.org/10.1021/acsomega.2c00160DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178719PMC
June 2022

Urban coral communities and water quality parameters along the coasts of Guangdong Province, China.

Mar Pollut Bull 2022 Jul 7;180:113821. Epub 2022 Jun 7.

Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, PR China; Department of Biology, Hong Kong Baptist University, Hong Kong, China; HKBU Shenzhen Institute of Research and Continuing Education (IRACE), Shenzhen, PR China. Electronic address:

Coral communities in China's Great Bay Area (GBA) have experienced severe degradation, but only limited information is available about their community structure. We surveyed 20 sites across three regions (Daya Bay, Dapeng Bay, Wanshan Islands) in GBA to provide an updated baseline of these urban coral communities. Live coral cover varied substantially, with the lowest values (<2 %) found inside the highly urbanized Daya Bay, and highest values (40-47 %) from offshore islands that are less affected by human activities. The two sites with the lowest live coral cover had a high percentage of dead coral. Five groups of coral communities could be identified, with most of them characterized by dominance of massive and encrusting coral species. Both coral cover and generic richness were negatively correlated with dissolved inorganic nitrogen in the water column, indicating that nutrient pollution could potentially constrain the development of these urban coral communities.
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http://dx.doi.org/10.1016/j.marpolbul.2022.113821DOI Listing
July 2022

Case Report: PTEN Mutation Induced by anti-PD-1 Therapy in Stage IV Lung Adenocarcinoma.

Front Pharmacol 2022 23;13:714408. Epub 2022 May 23.

Department of Radiotherapy, The Third Affiliated Hospital of Shandong First Medical University, Jinan, China.

Lung cancer is the most common solid tumor in the worldwide. Targeted therapy and immunotherapy are important treatment options in advanced non-small cell lung cancer (NSCLC). The association of PTEN mutation and tumor immunotherapy is less established for patients with NSCLC. We present the case of an Asian woman diagnosed with stage IV lung adenocarcinoma harboring an ERBB2 mutation. She received Nivolumab treatment when her disease progresses after previous chemotherapy and Afatinib treatment. However, the patient did not response to Nivolumab. PTEN mutation was detected by next-generation sequencing (NGS) after treatment with Nivolumab. PTEN, a secondary mutation, may be served as a biomarker of resistance to anti-PD-1 immunotherapy in lung adenocarcinoma. The relationship between PTEN mutation and immunotherapy is complex and needs further study.
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http://dx.doi.org/10.3389/fphar.2022.714408DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168362PMC
May 2022

Vibrio cholerae senses human enteric α-defensin 5 through a CarSR two-component system to promote bacterial pathogenicity.

Commun Biol 2022 06 8;5(1):559. Epub 2022 Jun 8.

The Second Clinical Medical College, Jinan University, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen People's Hospital, Shenzhen Institute of Respiratory Diseases, Shenzhen, Guangdong, PR China.

Vibrio cholerae (V. cholerae) is an aquatic bacterium responsible for acute and fatal cholera outbreaks worldwide. When V. cholerae is ingested, the bacteria colonize the epithelium of the small intestine and stimulate the Paneth cells to produce large amounts of cationic antimicrobial peptides (CAMPs). Human defensin 5 (HD-5) is the most abundant CAMPs in the small intestine. However, the role of the V. cholerae response to HD-5 remains unclear. Here we show that HD-5 significantly upregulates virulence gene expression. Moreover, a two-component system, CarSR (or RstAB), is essential for V. cholerae virulence gene expression in the presence of HD-5. Finally, phosphorylated CarR can directly bind to the promoter region of TcpP, activating transcription of tcpP, which in turn activates downstream virulence genes to promote V. cholerae colonization. In conclusion, this study reveals a virulence-regulating pathway, in which the CarSR two-component regulatory system senses HD-5 to activate virulence genes expression in V. cholerae.
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http://dx.doi.org/10.1038/s42003-022-03525-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178039PMC
June 2022

Emergence of an epidemic hypervirulent clone of Enterobacter hormaechei coproducing mcr-9 and carbapenemases.

Lancet Microbe 2022 Jul 1;3(7):e474-e475. Epub 2022 Jun 1.

Shenzhen Institute of Respiratory Diseases, Second Clinical Medical College (Shenzhen People's Hospital), Jinan University, Shenzhen, China; The First Affiliated Hospital (Shenzhen People's Hospital), Southern University of Science and Technology, Shenzhen, China. Electronic address:

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http://dx.doi.org/10.1016/S2666-5247(22)00122-7DOI Listing
July 2022

Potentiation of Vancomycin: Creating Cooperative Membrane Lysis through a "Derivatization-for-Sensitization" Approach.

J Am Chem Soc 2022 06 3;144(23):10622-10639. Epub 2022 Jun 3.

Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, and School of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China.

Gram-negative bacteria, especially the ones with multidrug resistance, post dire challenges to antibiotic treatments due to the presence of the outer membrane (OM), which blocks the entry of many antibiotics. Current solutions for such permeability issues, namely lipophilic-cationic derivatization of antibiotics and sensitization with membrane-active agents, cannot effectively potentiate the large, globular, and hydrophilic antibiotics such as vancomycin, due to ineffective disruption of the OM. Here, we present our solution for high-degree OM binding of vancomycin via a hybrid "derivatization-for-sensitization" approach, which features a combination of LPS-targeting lipo-cationic modifications on vancomycin and OM disruption activity from a sensitizing adjuvant. 10- to 10-fold potentiation of vancomycin and 20-fold increase of the sensitizer's effectiveness were achieved with a combination of a vancomycin derivative and its sensitizer. Such potentiation is the result of direct membrane lysis through cooperative membrane binding for the sensitizer-antibiotic complex, which strongly promotes the uptake of vancomycin and adds to the extensive antiresistance effectiveness. The potential of such derivatization-for-sensitization approach was also supported by the combination's potent antimicrobial efficacy in mouse model studies, and the expanded application of such strategy on other antibiotics and sensitizer structures.
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http://dx.doi.org/10.1021/jacs.2c03784DOI Listing
June 2022

Hydrothermal oxidation degradation of dioxins in fly ash with water-washing and added Ce-Mn catalyst.

J Environ Manage 2022 Sep 29;317:115430. Epub 2022 May 29.

School of Environment, Nanjing Normal University, Nanjing, 210046, PR China. Electronic address:

A comprehensive analysis of the effects of the temperature, reaction time, liquid-solid ratio (L/S), and initial pH on the hydrothermal degradation of polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) (which are both PCDD/Fs) in municipal solid waste incineration (MSWI) fly ash is presented. Consequently, the hydrothermal degradation reaction is catalyzed using Ce-Mn catalyst under low-temperature conditions to study the effect of the catalyst on the degradation efficiency of PCDD/Fs. The experimental results show that temperature is the most critical factor for the reaction. When the hydrothermal oxidation temperature reaches 280 °C (reaction time = 120 min, original pH = 8.5, L/S = 4 mL/g), the toxicity equivalent (I-TEQ) of PCDD/Fs is only 5.4 ng TEQ/kg, and the degradation efficiency reaches 99.71%. Under these conditions, 2,3,4,7,8-PCDF makes the highest contribution to I-TEQ degradation, reaching 37.4%. There are four main pathways for the reaction of 2,3,4,7,8-PCDF with hydroxyl radicals. A comparison of the PCDD/F concentrations of different products shows that the addition of 0.5%, 1.0%, and 1.5% of the Ce-Mn catalyst reduces the degradation efficiency by 8.79%, 1.40%, and 0.07%, respectively, which indicates that the addition of a small quantity of Ce-Mn catalyst does not facilitate the degradation of PCDD/Fs. The addition of the catalyst significantly decreases the degradation efficiency of low-chlorinated homologs but has a relatively small effect on that of high-chlorinated homologs. Therefore, it is concluded that Ce-Mn catalysts are more likely to promote resynthesis than degradation of PCDD/Fs.
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http://dx.doi.org/10.1016/j.jenvman.2022.115430DOI Listing
September 2022

Reduced Utilization Process Leaching Toxicity by Stabilizing Heavy Metals in Fly Ash from MSW Incineration Based on Hydrothermal Oxidation.

Bull Environ Contam Toxicol 2022 May 30. Epub 2022 May 30.

Nanjing Research Institute of Ecological and Environmental Protection, 175 Huju Road, Nanjing, 210013, China.

Hydrothermal oxidation is an effective approach to reduce leaching toxicity of fly ash from municipal solid waste incineration during utilization process. Herein, the effects of temperature, time, pH and Ce-Mn catalyst dosage on the stabilization of heavy metals in fly ash during hydrothermal oxidation were studied. The temperature of hydrothermal oxidation was positively correlated to the stabilization effect. However, the reaction time and pH emerged unstable effect. The amount of Ce-Mn catalyst had a slightly positive effect on the stabilizing at low doses, but it can be inhibited by excessive dose. The leaching concentrations of all heavy metals accorded with National Wastewater Discharge Standard of China (GB 8978-1996) under the optimal parameters, especially for Pb, Ni and Cu. The heavy metals were transformed from unstable fractions to residue fractions during hydrothermal process, among which the proportion of Cu and Zn residue fractions significantly increased and further reduced leaching toxicity.
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http://dx.doi.org/10.1007/s00128-022-03552-0DOI Listing
May 2022

A Novel Thunderbolt Z-Epicanthoplasty for Asians.

Aesthetic Plast Surg 2022 May 26. Epub 2022 May 26.

Department of Burn and Plastic Surgery, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Wuhou District, Chengdu City, 61000, Sichuan Province, People's Republic of China.

Background: Although many epicanthoplasty techniques have been proposed, prominent hypertrophic scarring in the medial canthal region remains a problem. The aim of this study was to develop a novel design that has a less prominent scar with minimal tension.

Methods: A total of 489 patients underwent thunderbolt Z-epicanthoplasty from July 2015 to April 2019, with or without blepharoplasty. A triangular myocutaneous flap was lifted from the upper part of the epicanthal fold. The surrounding area was dissected to remove the rigid connective tissue between the orbicularis muscle and the skin, which creates skin tension. A Z-shaped flap toward the inferomedial canthal portion was added to create space for the triangular flap to be transposed to change the straight incision into a curved zigzag incision (final scar in the shape of a "thunderbolt"), making the scar irregular and less conspicuous.

Results: Postoperatively, all patients were followed up for ≥ 12 months. Among the patients, epicanthus tarsalis (60.12%) and palpebralis (36.19%) were the commonest epicanthus types, followed by epicanthus supraciliaris (3.07%) and inversus (0.61%). The average preoperative intercanthal distance was 42.25 ± 1.66 mm. This distance decreased significantly to 37.14 ± 1.78 mm (average, 5.11 ± 0.21 mm; p = 0.036) at the 12-month postoperative follow-up. Mild cicatricial redness was observed in the medial canthal area in six patients (1.2%) during the early postoperative period. The redness diminished within 6 months postoperatively. All patients obtained natural and aesthetically pleasing results without prominent hypertrophic scarring or other complications in the medial canthal area.

Conclusion: The thunderbolt Z-epicanthoplasty is safe and effective for treating medial epicanthal folds. It is potentially helpful in minimizing postoperative medial canthal scarring and can be applied to various types of epicanthal folds with long-lasting results.

Level Of Evidence Iv: This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 . Non-Surgical Aesthetic IV.
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http://dx.doi.org/10.1007/s00266-022-02934-3DOI Listing
May 2022

A Redox-Activatable and Targeted Photosensitizing Agent to Deliver Doxorubicin for Combining Chemotherapy and Photodynamic Therapy.

Mol Pharm 2022 07 26;19(7):2441-2455. Epub 2022 May 26.

School of Pharmacy, Nanjing Tech University, 30th South Puzhu Road, Nanjing, 211816, China.

Currently, tumors have become a serious disease threatening human health and life in modern society. Photo-chemo combination therapy is considered to be an important method to improving the efficiency of tumor treatment, especially in the treatment of multi-drug-resistant tumors. However, the application of photo-chemo combination therapy has been limited by the poor water solubility of photosensitizers, low tumor targeting, and high side effects of chemotherapy drugs. In order to solve these problems, a smart nano drug delivery platform FA-PEG-ss-PLL(--Ce6) designed and synthesized by us. The smart nano drug carrier uses folic acid (FA) as the targeting group, polyethylene glycol (PEG) as the hydrophilic end, Ce6-grafted polylysine (PLL(--Ce6)) as the hydrophobic end, and Chlorin e6 (Ce6) as the photosensitizer of photodynamic therapy, and it connects PEG to PLL by a redox-responsive cleavable disulfide linker (-ss-). Finally, the combination of tumor chemotherapy and photodynamic therapy (PDT) is realized by loading with anticancer drug doxorubicin (DOX) to the intelligent carrier. In vitro experiments showed that the drug loading content (DLC%) of [email protected](--Ce6) nanoparticles ([email protected] NPs) was as high as 14.83%, and the nanoparticles had good serum stability, reduction sensitivity and hemocompatibility. From the cytotoxicity assays in vitro, we found that under 664 nm laser irradiation [email protected] NPs showed stronger toxicity to MCF-7 cells than did DOX, Ce6 + laser, and DOX + Ce6 + laser. Moreover, the antitumor efficiency in vivo and histopathological analysis showed that [email protected] NPs under 664 nm laser irradiation exhibited higher antitumor activity and lower systemic toxicity than single chemotherapy. These results suggested that the FA-PEG-ss-PLL(--Ce6) nano drug delivery platform has considerable potential for the combination of chemotherapy and PDT.
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http://dx.doi.org/10.1021/acs.molpharmaceut.1c00855DOI Listing
July 2022

Neutrophil Count Predicts Malignant Cerebellar Edema and Poor Outcome in Acute Basilar Artery Occlusion Receiving Endovascular Treatment: A Nationwide Registry-Based Study.

Front Immunol 2022 3;13:835915. Epub 2022 May 3.

Department of Neurology, Xinqiao Hospital and The Second Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, China.

Background: Acute basilar artery occlusion (ABAO) is known to have a poor outcome with a high rate of morbidity and mortality despite endovascular treatment (EVT), highlighting the necessities of exploring factors to limit the efficacy of EVT in these patients. Cerebellar infarctions in ABAO might progress to malignant cerebellar edema (MCE), a life-threatening complication after reperfusion, posing a secondary injury to the brainstem by mass effects. Therefore, the present research aimed to explore the impacts of MCE on a long-term outcome and investigate the prognostic factors for MCE among ABAO after EVT.

Methods: In the national BASILAR registry, a total of 329 ABO patients with cerebellar infarctions treated by EVT met the inclusion criteria. The presence of MCE defined by the Jauss scale ≥4 points, was evaluated on the computed tomography performed 72 h after EVT. The adjusted odds ratio and 95% CI were obtained by logistic regression models. A favorable outcome was defined as a 90-day modified Rankin Scale score of 0-3.

Results: MCE was statistically associated with the decreased incidence of a favorable outcome [adjusted odds ratio, 0.35(95% CI, 0.18-0.68), P=0.002]. The baseline National Institutes of Health Stroke Scale score, collateral circulation, neutrophil count at admission, and recanalization status were predictors for MCE and a favorable functional status at 90 days (all P<0.05). Among all inflammatory factors, the neutrophil count achieved the highest accuracy, sensitivity, and specificity for MCE. Adding the neutrophil count status into the baseline model obviously enhanced its prediction ability for MCE and favorable outcome by increasing the area under curve and achieving both net reclassification and integrated discrimination improvement (all P<0.05). Mediation analysis indicated that MCE mediated the association between the increased neutrophil count and worse functional outcome (P=0.026).

Discussion: MCE acted essential roles in worsening prognosis for ABAO after EVT. A high neutrophil count at admission was linked to MCE and a poor outcome among ABAO patients, which could be further incorporated into the clinical decision-making system and guide immunomodulation therapy.
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http://dx.doi.org/10.3389/fimmu.2022.835915DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9111017PMC
May 2022

Electropolymerized Molecularly Imprinted Polymer Synthesis Guided by an Integrated Data-Driven Framework for Cortisol Detection.

ACS Appl Mater Interfaces 2022 Jun 10;14(22):25972-25983. Epub 2022 May 10.

Department of Chemical Engineering, Michigan Technological University, 1400 Townsend Drive, Houghton, Michigan 49931, United States.

Molecularly imprinted polymers (MIPs), often called "synthetic antibodies", are highly attractive as artificial receptors with tailored biomolecular recognition to construct biosensors. Electropolymerization is a fast and facile method to directly synthesize MIP sensing elements on the working electrode, enabling ultra-low-cost and easy-to-manufacture electrochemical biosensors. However, due to the high dimensional design space of electropolymerized MIPs (e-MIPs), the development of e-MIPs is challenging and lengthy based on trial and error without proper guidelines. Leveraging machine learning techniques in building the quantitative relationship between synthesis parameters and corresponding sensing performance, e-MIPs' development and optimization can be facilitated. We herein demonstrate a case study on the synthesis of cortisol-imprinted polypyrrole for cortisol detection, where e-MIPs are fabricated with 72 sets of synthesis parameters with replicates. Their sensing performances are measured using a 12-channel potentiostat to construct the subsequent data-driven framework. The Gaussian process (GP) is employed as the mainstay of the integrated framework, which can account for various uncertainties in the synthesis and measurements. The Sobol index-based global sensitivity is then performed upon the GP surrogate model to elucidate the impact of e-MIPs' synthesis parameters on sensing performance and interrelations among parameters. Based on the prediction of the established GP model and local sensitivity analysis, synthesis parameters are optimized and validated by experiment, which leads to remarkable sensing performance enhancement (1.5-fold increase in sensitivity). The proposed framework is novel in biosensor development, which is expandable and also generally applicable to the development of other sensing materials.
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http://dx.doi.org/10.1021/acsami.2c02474DOI Listing
June 2022

QTL Analysis of Z414, a Chromosome Segment Substitution Line with Short, Wide Grains, and Substitution Mapping of qGL11 in Rice.

Rice (N Y) 2022 May 9;15(1):25. Epub 2022 May 9.

Rice Research Institute, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.

Most agronomic traits of rice (Oryza sativa), such as grain length, are complex traits controlled by multiple genes. Chromosome segment substitution lines (CSSLs) are ideal materials for dissecting these complex traits. We developed the novel rice CSSL 'Z414', which has short, wide grains, from progeny of the recipient parent 'Xihui 18' (an indica restorer line) and the donor parent 'Huhan 3' (a japonica cultivar). Z414 contains four substitution segments with an average length of 3.04 Mb. Z414 displays seven traits that significantly differ from those of Xihui 18, including differences in grain length, width, and weight; degree of chalkiness; and brown rice rate. We identified seven quantitative trait loci (QTL) that are responsible for these differences in an F population from a cross between Xihui 18 and Z414. Among these, six QTL (qPL3, qGW5, qGL11, qRLW5, qRLW11, and qGWT5) were detected in newly developed single-segment substitution lines (SSSLs) S1-S6. In addition, four QTL (qGL3, qGL5, qCD3, and qCD5) were detected in S1 and S5. Analysis of these SSSLs attributed the short, wide grain trait of Z414 to qGL11, qGL3, qGL5, and qGW5. Substitution mapping delimited qGL11 within an 810-kb interval on chromosome 11. Sequencing, real time quantitative PCR, and cell morphology analysis revealed that qGL11 might be a novel QTL encoding the cyclin CycT1;3. Finally, pyramiding qGL3 (a = 0.43) and qGL11 (a =  - 0.37) led to shorter grains in the dual-segment substitution line D2 and revealed that qGL11 is epistatic to qGL3. In addition, S1 and D2 exhibited different grain sizes and less chalkiness than Z414. In conclusion, the short grain phenotype of the CSSL Z414 is controlled by qGL11, qGL3, and qGL5. qGL11 might be a novel QTL encoding CycT1;3, whose specific role in regulating grain length was previously unknown, and qGL11 is epistatic to qGL3. S1 and D2 could potentially be used in hybrid rice breeding.
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http://dx.doi.org/10.1186/s12284-022-00571-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9085999PMC
May 2022

The GntR-like transcriptional regulator HutC involved in motility, biofilm-forming ability, and virulence in Vibrio parahaemolyticus.

Microb Pathog 2022 Jun 2;167:105546. Epub 2022 May 2.

Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Minhang, Shanghai, PR China. Electronic address:

As a halophilic food-borne pathogen, Vibrio parahaemolyticus continueo be a major health issue worldwide. The pathogenic mechanisms of V. parahaemolyticus are still not fully understood. One of the most abundant and widely distributed groups of helix-turn-helix transcription factors is the GntR family of regulators, which are involved in the regulation of various biological processes in bacteria, but little is known about their functions in V. parahaemolyticus. Here, we identified a gene designated as hutC in V. parahaemolyticus SH112 that encodes a member belongs to the HutC subfamily of the large GntR transcriptional regulator family. Compared to the wild type, the hutC mutant strain was significantly more sensitive to acid, bile salt, Triton X-100, and sodium dodecyl sulfate stresses. Our results showed that HutC is required for optimal swimming motility but not necessary for the swarming of V. parahaemolyticus. In addition, inactivation of hutC in V. parahaemolyticus SH112 led to decreased biofilm formation, reduced cytotoxicity in Coca-2 cells, and defective virulence in vivo compared to the wild-type strain. Furthermore, transcriptome sequencing (RNA-Seq) analysis and real-time PCR indicated 4 upregulated and 14 downregulated genes in the hutC mutant strain. Functional analysis revealed that 4 upregulated genes were related to the histidine metabolism pathway. The 14 downregulated genes were mostly related to the cellular metabolic process, binding, and membrane part. This study presents evidence that HutC is involved in bacterial survival under conditions of stress, swimming motility, biofilm formation, cytotoxicity, virulence, and gene regulation of V. parahaemolyticus during infection.
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http://dx.doi.org/10.1016/j.micpath.2022.105546DOI Listing
June 2022
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