Publications by authors named "Kai Wang"

4,059 Publications

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Perinatal DEHP exposure induces sex- and tissue-specific DNA methylation changes in both juvenile and adult mice.

Environ Epigenet 2021 10;7(1):dvab004. Epub 2021 May 10.

Department of Computational Medicine and Bioinformatics, University of Michigan, 500 S State St., Ann Arbor, MI 48109, USA.

Di(2-ethylhexyl) phthalate (DEHP) is a type of phthalate plasticizer found in a variety of consumer products and poses a public health concern due to its metabolic and endocrine disruption activities. Dysregulation of epigenetic modifications, including DNA methylation, has been shown to be an important mechanism for the pathogenic effects of prenatal exposures, including phthalates. In this study, we used an established mouse model to study the effect of perinatal DEHP exposure on the DNA methylation profile in liver (a primary target tissue of DEHP) and blood (a common surrogate tissue) of both juvenile and adult mice. Despite exposure ceasing at 3 weeks of age (PND21), we identified thousands of sex-specific differential DNA methylation events in 5-month old mice, more than identified at PND21, both in blood and liver. Only a small number of these differentially methylated cytosines (DMCs) overlapped between the time points, or between tissues (i.e. liver and blood), indicating blood may not be an appropriate surrogate tissue to estimate the effects of DEHP exposure on liver DNA methylation. We detected sex-specific DMCs common between 3-week and 5-month samples, pointing to specific DNA methylation alterations that are consistent between weanling and adult mice. In summary, this is the first study to assess the genome-wide DNA methylation profiles in liver and blood at two different aged cohorts in response to perinatal DEHP exposure. Our findings cast light on the implications of using surrogate tissue instead of target tissue in human population-based studies and identify epigenetic biomarkers for DEHP exposure.
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http://dx.doi.org/10.1093/eep/dvab004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107644PMC
May 2021

regulates the bone metabolism of human bone marrow mesenchymal stem cells from patients with idiopathic necrosis of femoral head through Wnt signaling pathways: A preliminary report.

Exp Ther Med 2021 Jul 22;22(1):666. Epub 2021 Apr 22.

Department of Orthopedics, Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. China.

Idiopathic necrosis of the femoral head (INFH) is a common disease with unknown cause. Its successful treatment relies on the repair of the necrotic bone. The application of autologous mesenchymal stem cells (MSCs) has shown great promise in saving the patients from undergoing total hip arthroplasty. Leucine-rich repeat-containing 17 () is less expressed in patients with femoral head necrosis and can inhibit bone degradation. However, it remains unknown whether plays a role in the pathogenesis of INFH. The present study aimed to investigate the potential role and mechanism of in the pathogenesis and treatment of INFH. It was found that despite the similar cell morphology and MSC surface marker expressions of human bone marrow MSCs (BMSCs) isolated from patients with INFH (INFH-hBMSC) and femoral neck fracture (FNF) (FNF-hBMSC), INFH-hBMSC had higher percentage of apoptosis (P<0.05), as well as lower osteogenic potential and higher adipogenic potential (both P<0.05). However, there was no difference in cell proliferation between FNF-hBMSC and INFH-hBMSC (P>0.05). It was also confirmed that the expression of was lower in the bone tissue and hBMSCs from patients with INFH compared with patients with FNF (P<0.05). Overexpression of promoted osteogenesis and inhibited the adipogenesis in hBMSCs, accompanied with the increase of Wnt3a and β-catenin expressions, and the decrease of Wnt5a and receptor activator of nuclear factor κ-B ligand (Rankl) expressions (all, P<0.05). Furthermore, knockout of in hBMSCs inhibited the expression levels of osteogenic and promoted adipogenic markers, while decreasing Wnt3a and β-catenin expressions, and increasing Wnt5a and Rankl expressions (all, P<0.05). The present preliminary study suggested that imbalanced bone metabolism may be involved in the pathogenesis of INFH. The modulation of the gene may delay or even restore the balance of osteogenic and adipogenic differentiation in autologous BMSCs derived from patients with INFH, providing a new target for the treatment of INFH.
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http://dx.doi.org/10.3892/etm.2021.10098DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8112125PMC
July 2021

Key Characteristics of Nitrous Oxide-Induced Neurological Disorders and Differences Between Populations.

Front Neurol 2021 27;12:627183. Epub 2021 Apr 27.

Department of Neurology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

Nitrous oxide (NO), commonly known as laughing gas, is inhaled recreationally because it produces the feelings of euphoria and freedom from pain. The risk of neurological dysfunction secondary to NO abuse and its clinical diagnosis are, however, not yet sufficiently recognized, especially in China. Here, we have summarized the key clinical characteristics of NO-induced neurological disorders. We recruited 20 patients with NO-induced neurological disorders and analyzed their clinical features, laboratory data, magnetic resonance imaging and electromyography. We also carried out a literature review and compared 99 previously reported patients with our case series to confirm our results. Subgroup analysis was performed to explore the difference in demographical and clinical characteristics of NO abuse between Asian and non-Asian patients. The most common initial symptoms of NO-induced neurological disorders were weakness and/or paresthesia. Most patients presented with myelopathy and/or peripheral neuropathy. The most commonly involved segment of the spinal cord was the cervical spinal cord, extending over 4-6 vertebral levels, but more than half of the patients with myelopathy had no sensory change at the corresponding spinal level. Homocysteine was found to be the most sensitive and practical indicator for diagnosis. Subgroup analysis showed that the Asian patients (median: 22.0 years old, Q1-Q3:19.0-26.0 years old) with NO abuse were younger than non-Asian patients [26.0 (22.3-31.0) years old, = 2.8 × 10]. The incidence of myelopathy combined with peripheral neuropathy was significantly higher in Asian patients than in non-Asian patients, who had myelopathy or peripheral neuropathy ( = 2 × 10). Key clinical characteristics of NO abuse are longitudinally extensive cervical myelopathy and peripheral neuropathy. Recognition of these traits in young people in the age group of 20-30 years will provide important guidance for accurate diagnosis of neurological disease associated with NO abuse. The clinical manifestations differ in Asian patients and non-Asian patients.
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http://dx.doi.org/10.3389/fneur.2021.627183DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110825PMC
April 2021

Hydrological management affected dissolved organic matter chemistry and organic carbon burial in the Three Gorges Reservoir.

Water Res 2021 Apr 29;199:117195. Epub 2021 Apr 29.

Organic Geochemistry Unit, Key Laboratory of Geoscience Big Data and Deep Resource of Zhejiang Province, School of Earth Sciences, Zhejiang University, Hangzhou, 310027, China. Electronic address:

With the linkage between dissolved organic matter (DOM) and the characteristics of natural ecosystem assessed extensively, the properties of DOM in reservoirs, the typical human interrupted ecosystems, have been focused on in recent years, which is critical for the understanding of human impacts on watershed ecosystems and carbon cycling. This study aims to analyze the effect of hydrological management on the DOM chemistry and organic carbon burial in Daning River tributary of the world's largest Three Gorges Reservoir (TGR). Based on the application of a combined approach including bulk geochemical analyses, optical spectroscopy, and ultrahigh-resolution Fourier transform ion cyclotron resonance mass spectrometry, various sources of DOM (terrestrial, anthropogenic, and autochthonous sources) were revealed. An increasing trend of terrestrial and recalcitrant DOM was observed along the upstream to downstream transect of Daning River tributary, which was mainly caused by the water intrusion with a higher terrestrial and recalcitrant signature from mainstream to tributary resulted from hydrological management of TGR. Integrated with the analysis of sedimentary organic matter in Daning River tributary in the past decade (after the construction of TGR), our work suggests that organic carbon burial in the reservoir could be enhanced by hydrological management-induced variation in DOM chemistry. Further studies are needed to better constrain the effects of damming reservoirs on carbon cycling considering their booming all over the world.
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http://dx.doi.org/10.1016/j.watres.2021.117195DOI Listing
April 2021

The effect of local intraoperative corticosteroid application on postoperative dysphagia following anterior cervical spine surgery.

Neurosurg Rev 2021 May 12. Epub 2021 May 12.

Department of Neurosurgery, Xuanwu Hospital, China International Neuroscience Institute, Capital Medical University, 45 Changchun Street, Beijing, 100053, China.

Dysphagia is a common complication following anterior cervical spine surgery (ACSS). Although several literatures have reported the potential benefit of local corticosteroid application on dysphagia, its safety and efficacy are still unclear. A systematic review was performed aiming to evaluate the evidence of local corticosteroid application in prevention or treatment of postoperative dysphagia following ACSS. A systematic search was performed in September 2018 in PubMed and Embase database. The following information was extracted: study investigator, year of publication, number of patients, study design, inclusion/exclusion criteria, administration protocol of steroid, type of surgical procedure, number of levels performed, assessment methodology of dysphagia, radiologic assessment of prevertebral soft tissue swelling (PSTS), follow-up time points, outcome of dysphagia, and corticosteroid-related complications. Qualitative synthesis was performed. Finally, 5 studies met the inclusion/exclusion criteria. Four studies found that local corticosteroid application could decrease the incidence and magnitude of postoperative dysphagia while 1 study showed no effect on dysphagia significantly at 6 weeks and 3 months follow-up time. A total of 2325 patients received local corticosteroid intraoperatively; no early corticosteroid-related complication was reported. Totally, 4 adverse events occurred in long-term follow-up time, including 2 bone nonunion at 1.5 and 2.5 years postoperatively, 2 esophageal perforation at 2 months and 11 months of follow-up, respectively. Local corticosteroid application can reduce the incidence and severity of dysphagia following ACSS without increasing early corticosteroid-related complications. But further high-quality study is necessary to analyze potential delayed complications.
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http://dx.doi.org/10.1007/s10143-019-01207-zDOI Listing
May 2021

Causal Effects of N-6 Polyunsaturated Fatty Acids on Age-related Macular Degeneration: A Mendelian Randomization Study.

J Clin Endocrinol Metab 2021 May 13. Epub 2021 May 13.

Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, Zhejiang Province, China.

Purpose: Although the role of n-6 polyunsaturated fatty acids (PUFAs) in age-related macular degeneration (AMD) has been studied in previous observational studies, the precise manner in which one or more n-6 PUFAs account for this relationship remains unclear. Using genetic instruments for n-6 PUFAs traits implemented through mendelian randomization (MR), we aimed to study possible causal associations between n-6 PUFAs and AMD.

Methods: The two-sample MR method was used to obtain unconfounded causal estimates. We selected genetic variants strongly associated (P < 5×10 -8) with circulating linoleic acid (LA) and arachidonic acid (AA) from a study involving 8,631 individuals and applied to an AMD case-control study (33,526 participants and 16,144 cases). The weighted median and MR Egger methods were used for the sensitivity analysis.

Results: Our MR analysis suggested that circulating LA was a causal protective factor for AMD, with an odds ratio (OR) estimate of 0.967 (95% confidence interval [CI] 0.945 to 0.990; P = 0.005) per percentage in total fatty acid increase in LA. In contrast, higher genetically predicted circulating AA causally increased the AMD risk (OR = 1.034; 95% CI 1.012 to 1.056; P = 0.002). Sensitivity analysis provided no indication of unknown pleiotropy. The findings from different single-nucleotide polymorphism selections and analytic methods were consistent, suggesting the robustness of the causal associations.

Main Conclusions: Our study provided genetic evidence that circulating LA accounted for protective effects of n-6 PUFAs against the risk of AMD, whereas AA was responsible for deleterious effects on higher AMD risk.
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http://dx.doi.org/10.1210/clinem/dgab338DOI Listing
May 2021

Comparison of C:N:P Stoichiometry in the Plant-Litter-Soil System Between Poplar and Elm Plantations in the Horqin Sandy Land, China.

Front Plant Sci 2021 26;12:655517. Epub 2021 Apr 26.

College of Environmental Sciences and Engineering, Liaoning Technical University, Fuxin, China.

Afforestation is among the most effective means of preventing and controlling desertification. Silver poplar () is commonly planted tree species for afforestation of the Horqin Sandy Land of China. However, this species has exhibited some drawbacks such as top shoot dieback, premature senescence and mortality, and soil and ecosystems degradation. In contrast, Siberian elm () rarely experiences these problems in the same regions. Ecological stoichiometry plays a vital role in exploring ecological processes and nutrient cycle relationships in plant-litter-soil systems. To explore the differences in the carbon (C), nitrogen (N), and phosphorus (P) balance, the stoichiometry characteristics and stoichiometric homeostasis in elm and poplar plantations in the Horqin Sandy Land, we measured C, N, and P concentrations in leaves, branches, roots, litter, and soils and analyzed N and P resorption efficiencies in the two plantations. The results showed that soil C and N concentrations, C:P, and N:P were greater in the elm plantation than in the poplar plantation. The leaf and root C:P and N:P during summer and litter N and P concentrations were greater, whereas N and P resorption efficiencies were lower, in the elm plantation than in the poplar plantation. Generally, elm exhibited greater N:P homeostasis than poplar. N and N:P homeostasis were greater in roots than in leaves and branches in the elm plantation, but they varied with soil N concentration and N:P in the poplar plantation. These findings indicate that poplar exhibited more developed internal nutrient conservation and allocation strategies but poor nutrient accumulation in soil, which may contribute to degradation of poplar plantation. In contrast, elm tended to return more nutrients to the soil, showing an improved nutrient cycle in the plant-litter-soil system and increased soil C and N accumulation in the elm plantation. Therefore, compared with poplar, elm may be a more suitable afforestation tree species for the Horqin Sandy Land, in terms of promoting the accumulation of soil nutrients and enhancing nutrient cycling in the plant-litter-soil system.
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http://dx.doi.org/10.3389/fpls.2021.655517DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107480PMC
April 2021

Application Value of Lipoprotein-Associated Phospholipase A2 Expression Level in Evaluating the Risk of Major Bleeding in Gestational Diabetes.

Clin Lab 2021 May;67(5)

Background: Studies have shown that obesity and lipid metabolism disorders can lead to increased Lp-PLA2 ac-tivity in the body. However, few studies have reported the correlation between Lp-PLA2 and postpartum hemorrhage in pregnant women with GMD.

Methods: Clinical data was collected from 43 pregnant women with gestational diabetes combined with postpartum hemorrhage and 50 pregnant women with postpartum diabetes without postpartum bleeding during the same period. Lp-PLA2 expression levels in the serum of pregnant women in both groups were detected. The predictive value of Lp-PLA2 expression level for postpartum hemorrhage was clarified.

Results: (1) From 20w to 32w pregnancy, the serum Lp-PLA2 levels of pregnant women in both groups showed a gradual increasing trend. (2) The serum Lp-PLA2 level of the pregnant women in the research group was significantly higher (p < 0.001). (3) Binary logistic regression analysis shows that Lp-PLA2 level has a good correlation with postpartum major bleeding. (4) Comparing of Lp-PLA2 levels in different gestational weeks with respect to its efficacy for predicting postpartum hemorrhage found that the sensitivity and specificity of 24w Lp-PLA2 levels are higher than other gestational weeks, and the area under the 24w parameter curve (0.955) > 32w area under the parameter curve (0.952) > area under the 20w parameter curve (0.940) > area under the 28w parameter curve (0.887). (5) The birth weight of the newborn in the research group was significantly heavier (p < 0.001).

Conclusions: Dynamic monitoring of the expression level of Lp-PLA2 in the serum of pregnant women can predict the risk of postpartum hemorrhage in pregnant women at an early stage and help optimize the rescue plan for postpartum hemorrhage.
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http://dx.doi.org/10.7754/Clin.Lab.2020.200906DOI Listing
May 2021

G2 and S phase-expressed-1 acts as a putative tumor promoter in cervical cancer by enhancing Wnt/β-catenin signaling via modulation of GSK-3β.

Environ Toxicol 2021 May 11. Epub 2021 May 11.

Department of Physiology and Pathophysiology, Air Force Medical University, Xi'an, China.

G2 and S phase-expressed-1 (GTSE1) is currently identified as a key regulator of carcinogenesis. However, the involvement of GTSE1 in cervical cancer is unclear. The aims of this work were to explore the relationship between GTSE1 and cervical cancer. Our data elucidated high GTSE1 expression in cervical cancer tissue, which predicted a poor prognosis in cervical cancer patients. GTSE1 knockdown had tumor-suppressive effects in cervical cancer cells by inhibiting cell proliferative and invasive abilities. GTSE1 knockdown decreased the level of phosphorylated glycogen synthase kinase-3β (GSK-3β) and active β-catenin, resulted in inactivation of Wnt/β-catenin signaling. Suppression of GSK-3β remarkably abolished the GTSE1-knockdown-induced inhibitory effects on Wnt/β-catenin signaling. Suppression of Wnt/β-catenin signaling abolished the GTSE1-overexpression-induced oncogenic effects. Notably, GTSE1 knockdown impeded the in vivo tumorigenicity of cervical cancer cells. In short, this work demonstrates that GTSE1 is overexpressed in cervical cancer and GTSE1 suppression exerts a tumor-inhibiting role in cervical cancer by down-regulating Wnt/β-catenin signaling. Our work underlines a crucial relevance between GTSE1 and cervical cancer progression and suggests GTSE1 as a promising therapeutic target for cervical cancer.
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http://dx.doi.org/10.1002/tox.23158DOI Listing
May 2021

Prostate stromal tumor with prostatic cysts after transurethral resection of the prostate: A case report.

World J Clin Cases 2021 Apr;9(12):2830-2837

Department of Urology, Affiliated Xiaoshan Hospital, Hangzhou Normal University, Hangzhou 311202, Zhejiang Province, China.

Background: A prostatic stromal tumor is deemed to be a rare oncology condition. Based on the retrospective analysis of clinical data and scientific literature review, a case of prostatic stromal tumor was reported in this article to explore the diagnosis, treatment and prognosis of this rare disease.

Case Summary: The present case involved an older male patient who was admitted to our department for a medical consultation of dysuria. Serum prostate-specific antigen was 8.30 ng/mL, Ultrasound and magnetic resonance imaging suggested evident enlargement of the prostate and multiple cystic developments internally. Considering that the patient was an elderly male with a poor health status, transurethral resection of the prostate was performed to improve the symptoms of urinary tract obstruction. Furthermore, based on histopathologic examination and immunohistochemical staining, the patient was pathologically diagnosed with prostatic stromal tumor. The patient did not receive any further adjuvant therapy following surgery leading to a clinical recommendation that the patient should be followed up on a long-term basis. However, during the recent follow-up assessment, the patient demonstrated recurrence of lower urinary tract symptoms and gross hematuria.

Conclusion: Referring to scientific literature review, we believe that the management of these lesions requires a thorough assessment of the patient. Furthermore, the treatment of prostate stromal tumors should be based on the imaging examination and pathological classification. Active surgical treatment is of great significance to the prognosis of patients, and subsequent surveillance after the treatment is warranted.
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http://dx.doi.org/10.12998/wjcc.v9.i12.2830DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058689PMC
April 2021

Facing coronavirus disease 2019: What do we know so far? (Review).

Exp Ther Med 2021 Jun 20;21(6):658. Epub 2021 Apr 20.

Department of Pharmacy, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.

Although the World Health Organization declared the outbreak of coronavirus disease 2019 (COVID-19), which originated in China, as a public health emergency of international concern as early as January 30, 2020, the current COVID-19 epidemic is spreading rapidly. As of April 19, 2020, total of 2,392,165 confirmed cases had been reported in 211 countries and regions, with 614,421 (25.68%) cured cases and 164,391 (6.87%) deaths. Scientists and clinicians have made great efforts to learn much about COVID-19 so that it can be controlled as soon as possible. Herein, this review will discuss the epidemiology, pathology, clinical features, diagnosis and treatment of COVID-19 based on the current evidence.
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http://dx.doi.org/10.3892/etm.2021.10090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097225PMC
June 2021

Deep-learning-enhanced light-field microscopy.

Authors:
Kai Wang

Nat Methods 2021 May;18(5):459-460

Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China.

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http://dx.doi.org/10.1038/s41592-021-01151-1DOI Listing
May 2021

Stage-dependent conditional survival and failure hazard of non-metastatic nasopharyngeal carcinoma after intensity-modulated radiation therapy: Clinical implications for treatment strategies and surveillance.

Cancer Med 2021 May 6. Epub 2021 May 6.

Department of Radiation Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Purpose: Conditional survival (CS) and failure hazard estimations can provide important dynamic prognostic information for clinical decision-making and surveillance counseling. The current study aimed to investigate the CS and dynamic failure hazard in non-metastatic nasopharyngeal carcinoma (NPC) treated with intensity-modulated radiotherapy (IMRT).

Methods: Conditional overall survival (COS) and progression-free survival (CPFS) estimates adjusted for age and gender against each AJCC 8th stage were calculated. Multivariable Cox regression (MCR) models were fitted in the entire population at baseline and subsequently separate MCR models were fitted in patients who have maintained event-free time of 1 to 10 years to generate respective hazard ratio (HR). Annual hazard rates of death and progression over 10 years for each stage were also estimated.

Results: A total of 1993 patients were eligible for analysis. The estimated 5-year OS and PFS for entire cohort were 79.0% and 70.7% at initial diagnosis. After 5 years of event-free follow-up, additional 5-year COS and CPFS increased to 85.9% and 85.5%, respectively. Stage I/II maintained dramatically favorable CS and low hazard (< 5%) of death and progression over time. Relative to stage I/II, stage III manifested non-significantly higher failure hazard for the first 3 years of survivorship and approached to similar level of stage I/II afterwards. Stage IVA presented most impressive improvement in terms of both COS (∆=9.8%) and CPFS (∆ = 16.8%) whereas still drastically inferior to that of stage I-III across all conditional time points. After 4 years of follow-up, progression hazard of stage IVA became relatively steady of approximate 6%.

Conclusions: Survival prospect of non-metastatic NPC improves over years with distinct dynamic patterns across stages, providing important implications for personalized decision-making in terms of both clinical management and surveillance counseling. Stage-dependent and hazard-adapted clinical management and surveillance are warranted.
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http://dx.doi.org/10.1002/cam4.3917DOI Listing
May 2021

BRD4 induces osteogenic differentiation of BMSCs via the Wnt/β-catenin signaling pathway.

Tissue Cell 2021 Apr 30;72:101555. Epub 2021 Apr 30.

Department of Joint Surgery, The Affiliated Hospital of Qingdao University, Qingdao, 266000, Shandong, China. Electronic address:

Bromodomain 4 (BRD4), an important epigenetic regulator, is involved in many bone-related pathologies via promoting osteoclast formation. However, whether and how it participates in the process of osteoblast formation remain unclear. This study aimed to investigate the potential role of BRD4 in osteogenic differentiation of bone marrow stromal cells (BMSCs). Our experiments revealed that an inhibitor of BRD4, JQ1, attenuated osteogenic differentiation of BMSCs. The recombinant adenoviruses for AdBRD4 and AdsiBRD4 could infect BMSCs with high efficiency. Exogenous BRD4 expression potentiated differentiation, and silencing endogenous BRD4 expression decreased it. In addition, the Wnt/β-catenin signaling pathway is known to be important for osteogenic differentiation. Our results showed that AdBRD4 increased the expressions of Wnt3a and β-catenin while AdsiBRD4 decreased the expressions. What's more, the recombinant adenovirus for Adsiβ-catenin, which obviously decreased in β-catenin expression, inhibited BRD4-induced osteogenic differentiation. Conclusion: Our data indicates that the epigenetic reader BRD4 participates in the process of BMSC osteogenic differentiation via the Wnt/β-catenin signaling pathway. This finding may pave the way into further understanding the mechanism of BMSC osteogenic differentiation.
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http://dx.doi.org/10.1016/j.tice.2021.101555DOI Listing
April 2021

Screening and Identification of LMNB1 and DLGAP5, Two Key Biomarkers in Gliomas.

Biosci Rep 2021 May 6. Epub 2021 May 6.

Insititute of Nervous System Diseases, Xuzhou Medical University, Xuzhou, China.

Glioma is the most common primary cancer in the central nervous system.  Despite advances in surgery, radiotherapy, and chemotherapy over the past decades, the prognosis of glioblastoma patients remains poor.  We aim to identify robust gene signatures to better understand the complex molecular mechanisms and to discover potential novel molecular biomarkers for glioma.  By exploring GSE16011, GSE4290 and GSE50161 data in Gene Expression Omnibus (GEO) database, we screened out 380 differentially expressed genes between non-tumor and glioma tissues, and further selected 30 hub genes through the Molecular Complex Detection (MCODE) plug-in in Cytoscape.  In addition, LMNB1 and DLGAP5 were selected for further analyses due to their high expression in gliomas and were verified by using our cohort.  Our study confirmed that LMNB1 and DLGAP5 were upregulated in gliomas and patients with high expression of LMNB1 or DLGAP5 had poor survival rate.  Furthermore, silence of LMNB1 and DLGAP5 inhibited the proliferation of glioma cells.  Together, LMNB1 and DLGAP5 were two potentially novel molecular biomarkers for diagnosis and prognosis of glioma.
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http://dx.doi.org/10.1042/BSR20210231DOI Listing
May 2021

A Network Pharmacology Approach to Predict the Proangiogenesis Mechanism of Huangqi-Honghua Herb Pair after Cerebral Ischemia.

Evid Based Complement Alternat Med 2021 14;2021:9834856. Epub 2021 Apr 14.

Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.

Objective: Huangqi-Honghua herb pair is known for its medicinal value to treat Qi deficiency and blood stasis syndrome with a long history in clinical practice. To understand its possible mechanism in a systematic study, a network pharmacological method was addressed.

Methods: Detailed information on the HH compounds was obtained from two public databases, and oral bioavailability (OB) and drug-like (DL) of the compounds were evaluated. A correlation between HH compounds, its potential targets, and known targets was extrapolated, and the herb-compound-target-disease (H-C-T-D) network was established. Next, the pathway enrichment and essential genes were analyzed. Then, three key genes (VEGFA, VEGFR2, and eNOS), highly associated with angiogenesis, were screened and verified through western blot assay.

Results: Out of 276 compounds, 21 HH compounds and 78 target genes regulating the major pathways associated with CI in the network are analyzed. The bioactive compounds in HH were active in various signal transduction pathways such as the toll-like receptor signaling pathway, VEGF signaling pathway, TNF signaling pathway, and HIF-1 signaling pathway are important pathways that may regulate anti-inflammatory, antiapoptotic, immune correlation, and antioxidative effects. The core genes are PTGS2, TNF, NOS2, IL6, BCL2, IL1B, SOD2, NOS3, SOD1, MMP9, and VEGFA. The in vitro results suggested that HH treatment could significantly elevate the expression of proangiogenic genes such as VEGFA, VEGFR2, and eNOS compared with OGD groups.

Conclusions: Our results predict that HH may regulate the expression of VEGFA, VEGFR2, and eNOS via the VEGF and HIF-1 signaling pathway to promote angiogenesis and alleviate cerebral ischemia injury.
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http://dx.doi.org/10.1155/2021/9834856DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064780PMC
April 2021

Ultrafast optical response and ablation mechanisms of molybdenum disulfide under intense femtosecond laser irradiation.

Light Sci Appl 2020 May 6;9(1):80. Epub 2020 May 6.

Department of Mechanical Engineering, University of Minnesota, Minneapolis, MN, 55455, USA.

Numerous valuable studies on electron dynamics have focussed on the extraordinary properties of molybdenum disulfide (MoS); however, most of them were confined to the level below the damage threshold. Here the electron dynamics of MoS under intense ultrafast laser irradiation was investigated by experiments and simulations. Two kinds of ablation mechanisms were revealed, which led to two distinct types of electron dynamics and final ablation morphology. At a higher fluence, the emergence of superheated liquid induced a dramatic change in the transient reflectivity and micro-honeycomb structures. At a lower fluence, the material was just removed by sublimation, and the ablation structure was relatively flat. X-ray photoelectron spectroscopic (XPS) measurements demonstrated that thermal decomposition only occurred at the higher fluence. Furthermore, a theoretical model was developed to deeply reveal the ultrafast dynamics of MoS ablation. The simulation results were in good agreement with the temporal and spatial reflectivity distribution obtained from the experiment. The electron and lattice temperature evolution was also obtained to prove the ablation mechanism. Our results revealed ultrafast dynamics of MoS above the damage threshold and are helpful for understanding the interaction mechanism between MoS and intense ultrafast lasers, as well as for MoS processing applications.
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http://dx.doi.org/10.1038/s41377-020-0318-8DOI Listing
May 2020

Risk prediction models for colorectal cancer: evaluating the discrimination due to added biomarkers.

Int J Cancer 2021 May 5. Epub 2021 May 5.

Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, MA, USA.

Most risk prediction models for colorectal cancer (CRC) are based on questionnaires and show a modest discriminatory ability. Therefore, we aim to develop risk prediction models incorporating plasma biomarkers for CRC to improve discrimination. We assessed the predictivity of 11 biomarkers in 736 men in the Health Professionals Follow-up Study and 639 women in the Nurses' Health Study. We used stepwise logistic regression to examine whether a set of biomarkers improved the predictivity on the basis of predictors in the National Cancer Institute's (NCI) Colorectal Cancer Risk Assessment Tool. Model discrimination was assessed using C-statistics. Bootstrap with 500 randomly sampled replicates was used for internal validation. The models containing each biomarker generated a C-statistic ranging from 0.50 to 0.59 in men and 0.50 to 0.54 in women. The NCI model demonstrated a C-statistic (95% CI) of 0.67 (0.62-0.71) in men and 0.58 (0.54-0.63) in women. Through stepwise selection of biomarkers, the C-statistic increased to 0.70 (0.66-0.74) in men after adding growth/differentiation factor 15, total adiponectin, sex hormone binding globulin, and tumor necrosis factor receptor superfamily member 1B (P for difference=0.008); and increased to 0.62 (0.57-0.66) in women after further including insulin-like growth factor 1 and insulin-like growth factor-binding protein 3 (P for difference=0.06). The NCI + selected biomarkers model was internally validated with a C-statistic (95% CI) of 0.73 (0.70-0.77) in men and 0.66 (0.61-0.70) in women. Circulating plasma biomarkers may improve the performance of risk factor-based prediction model for CRC.
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http://dx.doi.org/10.1002/ijc.33621DOI Listing
May 2021

Cordycepin inhibits the proliferation of malignant peripheral nerve sheath tumor cells through the p53/Sp1/tubulin pathway.

Am J Cancer Res 2021 15;11(4):1247-1266. Epub 2021 Apr 15.

Institute for Systems Biology Seattle, Washington, USA.

Neurofibromatosis type 1 (NF1) is one of the most common hereditary neurocutaneous disorders. In addition to skin pigmentation and cutaneous neurofibroma, some patients developed the plexiform neurofibroma since birth. Plexiform neurofibroma has abundant Schwann cells, fibroblasts, mast cells, blood vessels, and connective tissues, which increases the risk of developing a malignant peripheral nerve sheath tumor (MPNST). MPNST is a highly invasive cancer with no effective therapeutic agent. Cordycepin or 3'-deoxyadenosine is an extract from cordyceps militaris, which has been reported as an anti-inflammation and anti-tumor agent. Herein, we evaluated cordycepin's anti-proliferative effect on MPNST cell lines both and . Cordycepin inhibited the MPNST cell growth with an arrest of cell cycle at G2/M and S phases. The administration of naringin and pentostatin, inhibitors for adenosine deaminase (ADA), enzyme responsible for cordycepin degradation, did not show a synergistic effect in MPNST cells treated with cordycepin. However, the combined treatment enhanced the decrease of tumors in xenograft mouse model. Immunoblotting showed a decreased level of p53 protein in all MPNST cell lines, but S462TY cells. After cordycepin treatment, the levels of ERK, survivin, pAKT, and Sp1 proteins also decreased. The level of tubulin, but not actin or GAPDH, decreased in a dose-dependent manner. The microtubule network which is composed of tubulins was markedly decomposed in those treated MPNST cells. To elucidate the epigenetic control of transcription, ChIP-qPCR assay of the Sp1 and tubulin promoter regions revealed decreased Sp1 binding. The incorporation of 3'-doexyadenosine is detrimental for the process of poly(A) tail elongation. The poly(A) tail length assay showed the tail length in Sp1 and tubulin transcripts decreased in the treated cells. Nevertheless, the administration of SP1 protein to the treated cells could not rescue them completely. Furthermore, the p53-knocked-down cells (S462TY) where the expression of both p53 and Sp1 was suppressed, were vulnerable to cordycepin. The p53 protein could ameliorate the effect. In summary, cordycepin is effective to inhibit the growth of MPNST, probably through the pathway of p53/Sp1/tubulin.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085885PMC
April 2021

Decision making under ambiguity and risk in adolescent-onset schizophrenia.

BMC Psychiatry 2021 05 4;21(1):230. Epub 2021 May 4.

Anhui Province Key Laboratory of Cognition and Neuropsychiatric Disorders, Hefei, 230022, China.

Objective: Numerous studies have identified impaired decision making (DM) under both ambiguity and risk in adult patients with schizophrenia. However, the assessment of DM in patients with adolescent-onset schizophrenia (AOS) has been challenging as a result of the instability and heterogeneity of manifestations. The Iowa Gambling Task (IGT) and Game of Dice Task (GDT), which are frequently used to evaluate DM respectively under ambiguity and risk, are sensitive to adolescents and neuropsychiatric patients. Our research intended to examine the performance of DM in a relatively large sample of patients with AOS using the above-mentioned two tasks. We also aimed to take a closer look at the relationship between DM and symptom severity of schizophrenia.

Methods: We compared the performance of DM in 71 patients with AOS and 53 well-matched healthy controls using IGT for DM under ambiguity and GDT for DM under risk through net scores, total scores and feedback ration. Neuropsychological tests were conducted in all participants. Clinical symptoms were evaluated by using Positive and Negative Syndrome Scale (PANSS) in 71 patients with AOS. Pearson's correlation revealed the relationship among total score of DM and clinical and neuropsychological data.

Results: Compared to healthy controls, patients with AOS failed to show learning effect and had a significant difference on the 5th block in IGT and conducted more disadvantageous choices as well as exhibited worse negative feedback rate in GDT. Apart from DM impairment under risk, diminished DM abilities under ambiguity were found related to poor executive function in AOS in the present study.

Conclusions: Our findings unveiled the abnormal pattern of DM in AOS, mainly reflected under the risky condition, extending the knowledge on the performance of DM under ambiguity and risk in AOS. Inefficient DM under risk may account for the lagging impulse control and the combined effects of developmental disease. In addition, our study demonstrated that the performance on IGT was related to executive function in AOS.
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http://dx.doi.org/10.1186/s12888-021-03230-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8094464PMC
May 2021

Simultaneous or Staged Decompressions for Patients with Tandem Spinal Stenosis.

Orthop Surg 2021 May 4. Epub 2021 May 4.

Department of Orthopedics, The Second Hospital of Tianjin Medical University, Tianjin, China.

Objective: To compare the clinical effects of cervical decompression first, lumbar decompression first, or simultaneous decompression of both lesions in the treatment of tandem spinal stenosis (TSS).

Methods: This is a retrospective analysis. From January 2013 to December 2018, 51 TSS patients underwent our surgery and postoperative investigation. Among the 51 subjects, 27 females and 24 males, aged 49-77 years with an average age of 66.3 ± 6.8, were selected. According to the different operation sequences, all patients were divided into three groups. In simultaneous operation group, five patients underwent cervical and lumbar vertebrae surgery at the same time. In first cervical surgery group, 28 patients underwent cervical vertebra surgery first, followed by lumbar spine surgery after a period of recovery. And in first lumbar surgery group, 18 patients underwent lumbar vertebrae surgery first. The choice for neck surgery is posterior cervical single-door vertebroplasty, the surgery of lumber is plate excision and decompression needle-rod system internal fixation. The outcome measures are visual analogue scale (VAS), Japanese Orthopaedic Association cervical (JOA-C) and lumbar (JOA-L) scores, which were assessed at 3 months and 1 year after the operation by telephone interview. In addition, operative time, estimated blood loss, and hospital stay were also recorded.

Results: All the patients in the study had surgery performed successfully by the same group of orthopaedic surgeons. The preoperative VAS scores of simultaneous operation group, first cervical surgery group, and first lumbar surgery group were 8.00 ± 1.00, 8.36 ± 0.68, and 8.17 ± 0.71 (P > 0.05). The preoperative JOA-C scores were 7.00 ± 2.35, 6.54 ± 1.53, and 7.83 ± 1.04 (P < 0.05). And the preoperative JOA-L scores were 7.20 ± 2.17, 4.64 ± 2.36, and 5.78 ± 1.22 respectively (P < 0.05). During the final 1-year follow-up, the JOA-C improvement rates of simultaneous operation group, first cervical surgery group, and first lumbar surgery group were 85.68% ± 5.44%, 84.27% ± 5.02%, and 83.34% ± 10.25%, respectively (P > 0.05), and the JOA-L improvement rates were 80.04% ± 3.35%, 81.65% ± 3.74%, and 80.21% ± 4.76% (P > 0.05). The difference among them was not statistically significant. In addition, operation time (OP), blood loss (BL), and hospital stay (HS) in the simultaneous operation group were 245.00 ± 5.00 min, 480.00 ± 27.39 mL, and 16.60 ± 0.55 days, respectively. While those parameters in the first cervical surgery group were 342.50 ± 18.18 min, 528.21 ± 43.97 mL, and 22.75 ± 2.15 days, and in the first lumbar surgery group they were 346.11 ± 24.77 min, 519.44 ± 43.99 mL, and 22.89 ± 1.64 days. The average blood loss in simultaneous operation group was less (P > 0.05); meanwhile, the operation time and hospital stay time were significantly shorter in the simultaneous operation group than in the first cervical surgery group and first lumbar surgery group (P < 0.05). Only one case of fat liquefaction occurred in first cervical surgery group, which healed spontaneously after a regular change of dressing for 1 month.

Conclusions: Under the condition of ensuring the surgical effect, the choice of staged surgery or concurrent surgery according to the patients' own symptoms of cervical and lumbar symptoms could both obtain satisfactory results, and the damage of simultaneous surgery was less than that of staged surgery.
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http://dx.doi.org/10.1111/os.12906DOI Listing
May 2021

Emerging functions of piwi-interacting RNAs in diseases.

J Cell Mol Med 2021 May 4. Epub 2021 May 4.

Institute for Translational Medicine, The Affiliated Hospital of Qingdao University, College of Medicine, Qingdao University, Qingdao, China.

PIWI-interacting RNAs (piRNAs) are recently discovered small non-coding RNAs consisting of 24-35 nucleotides, usually including a characteristic 5-terminal uridine and an adenosine at position 10. PIWI proteins can specifically bind to the unique structure of the 3' end of piRNAs. In the past, it was thought that piRNAs existed only in the reproductive system, but recently, it was reported that piRNAs are also expressed in several other human tissues with tissue specificity. Growing evidence shows that piRNAs and PIWI proteins are abnormally expressed in various diseases, including cancers, neurodegenerative diseases and ageing, and may be potential biomarkers and therapeutic targets. This review aims to discuss the current research status regarding piRNA biogenetic processes, functions, mechanisms and emerging roles in various diseases.
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http://dx.doi.org/10.1111/jcmm.16466DOI Listing
May 2021

Risk factors for early-onset seizures after stroke: A systematicreview and meta-analysis of 18 observational studies.

Brain Behav 2021 May 4:e02142. Epub 2021 May 4.

Shaanxi University of Chinese Medicine, Shaanxi, China.

Objectives: To systematically evaluate the risk factors of early-onset seizures after stroke, in order to better provide evidence-based results for early detection, identification, targeted prevention, and treatment of this disease.

Methods: PubMed, EMBASE, The Cochrane Library, CNKI, and WanFang databases were searched to collect relevant studies on the risk factors of early-onset seizures after stroke from January 2010 to January 2020. Meta-analysis of all included studies was performed by using RevMan version 5.3 and Stata version 14.0 software.

Results: Eighteen case-control studies with a total sample size of 13,289 cases, including 813 cases with early-onset seizures after stroke, and 12,476 cases with non-early-onset seizures after stroke were included. The results of meta-analysis showed that cortical involvement [Odds Ratio (OR) = 5.00, 95%Confidence Interval (CI) (2.85, 8.74), p < .00001], cerebral infarction with hemorrhagic transformation [OR = 2.77, 95%CI (1.87, 4.11), p < .00001] and intracerebral hemorrhage [OR = 1.83, 95%CI (1.13, 2.97), p = .01]-related factors showed greater association with the occurrence of early-onset seizures after stroke.

Conclusions: These findings suggest that cortical involvement, intracerebral hemorrhage, and cerebral infarction with hemorrhagic transformation are important predictors and risk factors for early seizures after stroke, while the patient's gender, age, NHISS score, alcoholism, smoking, high blood pressure, diabetes, atrial fibrillation, dyslipidemia, receiving surgical treatment, and reperfusion therapy showed no association with the occurrence of early-onset seizures after stroke.
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http://dx.doi.org/10.1002/brb3.2142DOI Listing
May 2021

Unusual characteristics of the carbon cycle during the 2015-2016 El Niño.

Glob Chang Biol 2021 May 1. Epub 2021 May 1.

Department of Biology and Graduate Degree Program in Ecology, Colorado State University, Fort Collins, USA.

The 2015-2016 El Niño was one of the strongest on record, but its influence on the carbon balance is less clear. Using Northern Hemisphere atmospheric CO observations, we found both detrended atmospheric CO growth rate (CGR) and CO seasonal-cycle amplitude (SCA) of 2015-2016 were much higher than that of other El Niño events. The simultaneous high CGR and SCA were unusual, because our analysis of long-term CO observations at Mauna Loa revealed a significantly negative correlation between CGR and SCA. Atmospheric inversions and terrestrial ecosystem models indicate strong northern land carbon uptake during spring but substantially reduced carbon uptake (or high emissions) during early autumn, which amplified SCA but also resulted in a small anomaly in annual carbon uptake of northern ecosystems in 2015-2016. This negative ecosystem carbon uptake anomaly in early autumn was primarily due to soil water deficits and more litter decomposition caused by enhanced spring productivity. Our study demonstrates a decoupling between seasonality and annual carbon cycle balance in northern ecosystems over 2015-2016, which is unprecedented in the past five decades of El Niño events.
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http://dx.doi.org/10.1111/gcb.15669DOI Listing
May 2021

Functional characterization of four TIR domain-containing adaptors, MyD88, TRIF, MAL, and SARM in mandarin fish Siniperca chuatsi.

Dev Comp Immunol 2021 Apr 29;122:104110. Epub 2021 Apr 29.

State Key Laboratory of Freshwater Ecology and Biotechnology, And Key Laboratory of Aquaculture Disease Control, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China; The Innovation Academy of Seed Design, Chinese Academy of Sciences, Wuhan, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, Shandong Province, 266237, China; School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong Province, 266109, China. Electronic address:

Toll/interleukin-1 receptor (TIR) domain-containing adaptors, serve as pivotal signal transduction molecules in Toll-like receptor (TLR) signalling pathway to mediate downstream signalling cascades. In this study, four TIR-domain containing adaptors, MyD88, TRIF, MAL and SARM, were identified in mandarin fish Siniperca chuatsi, and they all contain TIR domains, of which MyD88 and SARM had high sequence homology with their vertebrate homologues. The expression analysis at mRNA level indicated that these genes were ubiquitously distributed in different tissues, being high in immune- and mucosa-related tissues such as head-kidney and intestine. The transcripts of these adaptor genes were up-regulated by poly(I:C) and LPS stimulation in isolated head-kidney lymphocytes (HKLs) of mandarin fish. Fluorescence microscopy revealed that all these molecules were localized in cytoplasm, and further investigations showed that the over-expression of MyD88, TRIF and MAL activated the NF-κB, ISRE or type Ι IFN promoters and inhibited SVCV replication, whereas their antiviral effects were significantly impaired when co-transfected with SARM. It was also confirmed by co-immunoprecipitation (Co-IP) that SARM interacts separately with MyD88, TRIF and MAL, and MAL interacts with MyD88. However, the regulatory mechanisms of these adaptors involved in signalling pathways of different TLRs should be of interest for further research.
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http://dx.doi.org/10.1016/j.dci.2021.104110DOI Listing
April 2021

Associative memory improvement after 5 days of magnetic stimulation: A replication experiment with active controls.

Brain Res 2021 Apr 29;1765:147510. Epub 2021 Apr 29.

Department of Neurology, The First Affiliated Hospital of Anhui Medical University, The School of Mental Health and Psychological Sciences, Anhui Medical University, Hefei, 230032, China; Institute of Artificial Intelligence, Hefei Comprehensive National Science Center, Hefei 230088, China. Electronic address:

Associative memory (AM) is an essential function of everyday life, but is often disrupted in many neurological diseases. Recent studies have found that repetitive transcranial magnetic stimulation (rTMS) can effectively enhance AM and have shown its potential in clinical applications. In this study, we aimed to reproduce the 5-day rTMS effect on AM in a Chinese version of a face-cued word recall task. In an open-label experiment, AM scores were significantly improved after active 20-Hz rTMS on individualized inferior parietal lobule (IPL) targets. To exclude the placebo effect, we performed a second experiment and added rTMS of the pre-supplementary motor area (preSMA) as an active control. In this within-subject crossover experiment, participants received active rTMS on IPL and preSMA targets, separated by at least 2 weeks. A Stroop task was included as a control test, which was more likely to be modulated by preSMA stimulations. We found that stimulations on IPL targets significantly improved AM, but this change did not significantly higher than that induced by preSMA stimulations. No significant change in Stroop measures were found in either IPL or preSMA condition. In summary, this study did not support that the 5 days of rTMS on individualized IPL targets could improve AM more than placebo rTMS. Further work is required to improve the rTMS paradigms to enhance the aftereffects in memory.
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http://dx.doi.org/10.1016/j.brainres.2021.147510DOI Listing
April 2021

GSTZ1 sensitizes hepatocellular carcinoma cells to sorafenib-induced ferroptosis via inhibition of NRF2/GPX4 axis.

Cell Death Dis 2021 Apr 30;12(5):426. Epub 2021 Apr 30.

Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.

Increasing evidence supports that ferroptosis plays an important role in tumor growth inhibition. Sorafenib, originally identified as an inhibitor of multiple oncogenic kinases, has been shown to induce ferroptosis in hepatocellular carcinoma (HCC). However, some hepatoma cell lines are less sensitive to sorafenib-induced ferroptotic cell death. Glutathione S-transferase zeta 1 (GSTZ1), an enzyme in the catabolism of phenylalanine, suppresses the expression of the master regulator of cellular redox homeostasis nuclear factor erythroid 2-related factor 2 (NRF2). This study aimed to investigate the role and underlying molecular mechanisms of GSTZ1 in sorafenib-induced ferroptosis in HCC. GSTZ1 was significantly downregulated in sorafenib-resistant hepatoma cells. Mechanistically, GSTZ1 depletion enhanced the activation of the NRF2 pathway and increased the glutathione peroxidase 4 (GPX4) level, thereby suppressing sorafenib-induced ferroptosis. The combination of sorafenib and RSL3, a GPX4 inhibitor, significantly inhibited GSTZ1-deficient cell viability and promoted ferroptosis and increased ectopic iron and lipid peroxides. In vivo, the combination of sorafenib and RSL3 had a synergic therapeutic effect on HCC progression in Gstz1 mice. In conclusion, this finding demonstrates that GSTZ1 enhanced sorafenib-induced ferroptosis by inhibiting the NRF2/GPX4 axis in HCC cells. Combination therapy of sorafenib and GPX4 inhibitor RSL3 may be a promising strategy in HCC treatment.
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http://dx.doi.org/10.1038/s41419-021-03718-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087704PMC
April 2021

A neuralized feature engineering method for entity relation extraction.

Neural Netw 2021 Apr 16;141:249-260. Epub 2021 Apr 16.

Xi'an Jiaotong University, Xi'an, China. Electronic address:

Making full use of semantic and structure information in a sentence is critical to support entity relation extraction. Neural networks use stacked neural layers to perform designated feature transformations and can automatically extract high-order abstract feature representations from raw inputs. However, because a sentence usually contains several pairs of named entities, the networks are weak when encoding semantic and structure information of a relation instance. In this paper, we propose a neuralized feature engineering approach for entity relation extraction. This approach enhances the neural network by manually designed features, which have the advantage of using prior knowledge and experience developed in feature-based models. Neuralized feature engineering encodes manually designed features into distributed representations to increase the discriminability of a neural network. Experiments show that this approach considerably improves the performance compared to that of neural networks or feature-based models alone, exceeding state-of-the-art performance by more than 8% and 16.5% in terms of F1-score on the ACE corpus and the Chinese literature text corpus, respectively.
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http://dx.doi.org/10.1016/j.neunet.2021.04.010DOI Listing
April 2021

A neuralized feature engineering method for entity relation extraction.

Neural Netw 2021 Apr 16;141:249-260. Epub 2021 Apr 16.

Xi'an Jiaotong University, Xi'an, China. Electronic address:

Making full use of semantic and structure information in a sentence is critical to support entity relation extraction. Neural networks use stacked neural layers to perform designated feature transformations and can automatically extract high-order abstract feature representations from raw inputs. However, because a sentence usually contains several pairs of named entities, the networks are weak when encoding semantic and structure information of a relation instance. In this paper, we propose a neuralized feature engineering approach for entity relation extraction. This approach enhances the neural network by manually designed features, which have the advantage of using prior knowledge and experience developed in feature-based models. Neuralized feature engineering encodes manually designed features into distributed representations to increase the discriminability of a neural network. Experiments show that this approach considerably improves the performance compared to that of neural networks or feature-based models alone, exceeding state-of-the-art performance by more than 8% and 16.5% in terms of F1-score on the ACE corpus and the Chinese literature text corpus, respectively.
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http://dx.doi.org/10.1016/j.neunet.2021.04.010DOI Listing
April 2021