Publications by authors named "Jun Xiang"

303 Publications

A Machine Learning Model for Predicting a Major Response to Neoadjuvant Chemotherapy in Advanced Gastric Cancer.

Front Oncol 2021 1;11:675458. Epub 2021 Jun 1.

Department of Gastrointestinal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Aims: To develop and validate a model for predicting major pathological response to neoadjuvant chemotherapy (NAC) in advanced gastric cancer (AGC) based on a machine learning algorithm.

Method: A total of 221 patients who underwent NAC and radical gastrectomy between February 2013 and September 2020 were enrolled in this study. A total of 144 patients were assigned to the training cohort for model building, and 77 patients were assigned to the validation cohort. A major pathological response was defined as primary tumor regressing to ypT0 or T1. Radiomic features extracted from venous-phase computed tomography (CT) images were selected by machine learning algorithms to calculate a radscore. Together with other clinical variables selected by univariate analysis, the radscores were included in a binary logistic regression analysis to construct an integrated prediction model. The data obtained for the validation cohort were used to test the predictive accuracy of the model.

Result: A total of 27.6% (61/221) patients achieved a major pathological response. Five features of 572 radiomic features were selected to calculate the radscores. The final established model incorporates adenocarcinoma differentiation and radscores. The model showed satisfactory predictive accuracy with a C-index of 0.763 and good fitting between the validation data and the model in the calibration curve.

Conclusion: A prediction model incorporating adenocarcinoma differentiation and radscores was developed and validated. The model helps stratify patients according to their potential sensitivity to NAC and could serve as an individualized treatment strategy-making tool for AGC patients.
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http://dx.doi.org/10.3389/fonc.2021.675458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204104PMC
June 2021

Co-hydrothermal carbonization of swine manure and cellulose: Influence of mutual interaction of intermediates on properties of the products.

Sci Total Environ 2021 May 29;791:148134. Epub 2021 May 29.

School of Material Science and Engineering, University of Jinan, Jinan 250022, China. Electronic address:

Co-hydrothermal carbonization (HTC) of livestock manure and biomass might improve the fuel properties of the hydrochar due to the high reactivity of the biomass-derived intermediates with the abundant oxygen-containing functionalities. However, the complicated compositions make it difficult to explicit the specific roles of the individual components of biomass played in the co-HTC process. In this study, cellulose was used for co-HTC with swine manure to investigate the influence on the properties of the hydrochar. The yield of hydrochar obtained from co-HTC reduced gradually with the cellulose proportion increased, and the solid yield was lower than the theoretical value. This was because the cellulose-derived intermediates favored the stability of the fragments from hydrolysis of swine manure. The increased temperature resulted in the reduction of the hydrochar yield whereas the prolonged time enhanced the formation of solid product. The interaction of the co-HTC intermediates facilitated the formation of O-containing species, thus making the solid more oxygen- and hydrogen-rich with a higher volatility. In addition, the co-HTC affected the evolution of functionalities like -OH and CO during the thermal treatment of the hydrochar and altered its morphology by stuffing the pores from swine manure-derived solid with the microspheres from HTC of cellulose. The interaction of the varied intermediates also impacted the formation of amines, ketones, carboxylic acids, esters, aromatics and the polymeric products in distinct ways.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148134DOI Listing
May 2021

Retrieval of 30 Lymph Nodes Is Mandatory for Selected Stage II Gastric Cancer Patients.

Front Oncol 2021 30;11:593470. Epub 2021 Apr 30.

Department of Gastrointestinal Surgery, The Sixth Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.

Background: According to the 8th edition AJCC staging manual, a least of 16 lymph nodes retrieval (LNRs) is the minimal requirement for lymph nodes (LNs) dissection of gastric cancer surgery. Previous studies have shown that increasing the number of LNRs (≥30) prolongs survival for selected patients. However, the necessity of retrieving 30 or more LN for stage II gastric cancer patients is still under debate.

Aim: This study aims to explore the impact of retrieving 30 or more lymph nodes on the survival of stage II cancer patients.

Methods: A total of 1,177 patients diagnosed with stage II gastric cancer were enrolled in this study. The clinicopathological parameters and the impact of different LNRs (<30 or ≥30) and positive lymph node ratio (NR) on overall survival (OS) were retrospectively analyzed.

Results: The mean number of LNRs was 34 ± 15. A total of 44% (518/1,177) of patients had an LNRs <30, while 56% (659/1,177) of patients had an LNRs ≥30. The 5-year survival rate was 81% for all patients, 76% for the LNRs <30 group, and 86% for LNRs ≥30 group, respectively (P = 0.003). The survival benefit of retrieving 30 lymph nodes was significant in certain subgroups: age >60 years/male/underwent total gastrectomy/stage IIB. For N+ patients, higher NR was significantly correlated with poor survival.

Conclusion: The survival benefit of retrieving 30 LNs varies in different subgroups. An LNRs of 30 is mandatory for selected stage II gastric cancer patients.
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http://dx.doi.org/10.3389/fonc.2021.593470DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121255PMC
April 2021

growth of ZIF-8 on gold nanoparticles/magnetic carbon nanotubes for the electrochemical detection of bisphenol A.

Anal Methods 2021 05;13(20):2338-2344

College of Chemistry and Chemical Engineering, Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, Central South University, Changsha 410083, China.

We herein report a facile and scalable strategy for the fabrication of a metal-organic framework (MOF) based composite by in situ growing ZIF-8 on gold nanoparticle (AuNP) loaded magnetic carbon nanotubes (mCNTs). AuNPs were firstly loaded on PEI (polyethylenimine) modified mCNTs by electrostatic forces, and then AuNPs/mCNTs were encapsulated into the ZIF-8 frame through in situ self-assembling of zinc ions and 2-methylimidazole. The morphology, spectroscopy and structural properties of the AuNP/[email protected] nanocomposites were systematically characterized. The conductivity-strain tests revealed that the in situ insertion of AuNPs/mCNTs in ZIF-8 could not only shorten the electron transfer distance between active sites and mCNTs, but also increase the dispersion of mCNTs, which would benefit the electron and mass transfer. Besides, by adopting the AuNP/[email protected] nanocomposite-modified glassy carbon electrode (GCE) as the working electrode, a novel electrochemical sensor was successfully developed for the detection of bisphenol A (BPA). A linear range of BPA detection from 1 μM to 100 μM with a limit of detection of 690 nM was favorably obtained. Moreover, the developed sensor exhibited satisfactory reproducibility and superior stability with excellent anti-interference ability, and was successfully applied in the detection of BPA in real samples.
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http://dx.doi.org/10.1039/d1ay00324kDOI Listing
May 2021

Authors' response.

Am J Orthod Dentofacial Orthop 2021 04;159(4):407-408

Morgantown, WVa, and New Hope, Pa, and Cleveland, Ohio.

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http://dx.doi.org/10.1016/j.ajodo.2020.12.005DOI Listing
April 2021

Skin-inspired nanofibrillated cellulose-reinforced hydrogels with high mechanical strength, long-term antibacterial, and self-recovery ability for wearable strain/pressure sensors.

Carbohydr Polym 2021 Jun 3;261:117894. Epub 2021 Mar 3.

College of Biomass Science and Engineering, Sichuan University, Chengdu, 610065, PR China; National Engineering Research Center of Clean Technology in Leather Industry, Sichuan University, Chengdu, 610065, PR China. Electronic address:

The advent of electric skins (E-skin) with tactile sensation, flexibility, and human affinity characteristics have attracted considerable attention in extensive research fields, including intelligent robots and health monitoring, etc. To improve the intrinsic brittleness of hydrogels, a multifunctional E-skin was fabricated involving a TEMPO-NFC and a covalently cross-linked polyacrylamide (PAM) network. In this work, silver nanoparticles (AgNPs) as long-term antibacterial agent and conductive fillers were coated onto NFC nanofibers. Subsequently, this nanocomposite hydrogel was synthesized by free radical copolymerization of AM monomers with PNAg fibers as interpenetrating fibers network. Importantly with NFC present, the nanocomposite hydrogel exhibited superior mechanical performance and excellent self-recovery ability. The obtained sensor with excellent mechanical stability and sensing performance could detect mechanotransduction signal of human movements. This work provides a practicable method to prepare high antibacterial efficiency, excellent mechanical performance, and dual-modal nanocellulose-based hydrogel sensor for the broad-range application in human-motion detection and intelligence skins.
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http://dx.doi.org/10.1016/j.carbpol.2021.117894DOI Listing
June 2021

Multidisciplinary team consultation for resectable Gastric Cancer: A propensity score matching analysis.

J Cancer 2021 30;12(7):1907-1914. Epub 2021 Jan 30.

Department of Gastrointestinal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510655, China.

Previous studies proposed that the multidisciplinary team (MDT) consultation could improve tumor staging accuracy and outcomes of patients with gastric malignancy. However, evidence-based reports remain limited. This study aimed to determine the effectiveness of MDT for tumor staging accuracy and outcomes of patients with resectable gastric cancer, and to explore the potential factors affecting its effectiveness. This retrospective study enrolled 719 gastric cancer patients who underwent gastrectomy in our hospital. After propensity score matching, 378 patients were selected, including 189 in the non-MDT group and 189 in the MDT group. Data regarding baseline characteristics, staging, treatments, and survival were analyzed. The data showed that the staging accuracy in the MDT group and non-MDT group was comparable (53% vs 61% for T stage, 46.1% vs 35.3% for N stage, and 78.3% vs 78.7% for M stage). The MDT group had a higher proportion of preoperative chemotherapy (39.2% vs 28%, p=0.03) and laparoscopic surgery (82.5% vs 72%, p=0.02) than the non-MDT group. However, the achievement of R0 resection was similar in the two groups (93.7% vs 88.9%, p=0.73). There was no significant difference in the 1-year and 3-year overall survival rates between the two groups. Moreover, we observed poor patient compliance when the MDT recommended further examinations, radiotherapy, or chemotherapy before surgical interventions. MDT consultation has limited effects on improving the staging accuracy and treatment outcomes including survival of patients with resectable gastric cancer. Poor patient compliance may be a factor affecting the effectiveness of MDT consultation.
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http://dx.doi.org/10.7150/jca.53365DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7974536PMC
January 2021

Thiamine combined with vitamin C in sepsis or septic shock: a systematic review and meta-analysis.

Eur J Emerg Med 2021 Jun;28(3):189-195

Emergency Department, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing.

Background: Thiamine and vitamin C have been increasingly used in patients with sepsis or septic shock because of their potential for improving metabolism and reducing mortality.

Objective: We aim to determine if thiamine combined vitamin C can reduce mortality in patients with sepsis or septic shock.

Evidence Sources And Study Selection: We comprehensively searched the PubMed, Embase, Cochrane Library, and Web of Science databases from their inception dates through 1 January 2021. Literature works evaluating the efficacy of thiamine combined vitamin C in patients with sepsis or septic shock were considered.

Data Extraction And Outcome Measurements: Two reviewers extracted data and assessed study quality. A meta-analysis was performed to calculate an odds ratio (OR), 95% confidence intervals (CIs), and P values for in-hospital mortality (primary outcome). Secondary outcomes included duration of ICU stay, duration of hospital stay, duration of vasopressor use, and change in sequential organ failure assessment (SOFA) scores.

Results: Seven randomized controlled trials were identified, encompassing a total of 868 patients. There was no statistical difference between groups for in-hospital mortality (OR: 1.11; 95% CI [0.79-1.56]; P = 0.55). Other than improving SOFA score during the first 72 h after enrollment and duration of vasopressor use, we found no other significant associations.

Conclusions: Despite widespread enthusiasm for thiamine combined with vitamin C for sepsis and septic shock, we only found an association with reduced SOFA score and time of vasopressor use. There was no association with in-hospital mortality.
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http://dx.doi.org/10.1097/MEJ.0000000000000812DOI Listing
June 2021

Identification of differentially expressed genes and signaling pathways in papillary thyroid cancer: a study based on integrated microarray and bioinformatics analysis.

Gland Surg 2021 Feb;10(2):629-644

Department of Head and Neck Surgery, Fudan University Shanghai Cancer Center, Shanghai, China.

Background: The techniques of DNA microarray and bioinformatic analysis have exhibited efficiency in identifying dysregulated gene expression in human cancers. In this study, we used integrated bioinformatics analysis to improve our understanding of the pathogenesis of papillary thyroid cancer (PTC).

Methods: In this study, we integrated four Gene Expression Omnibus (GEO) datasets, GSE33630, GSE35570, GSE60542 and GSE29265, including 136 normal samples and 157 PTC specimens. The contents of the four datasets are based on GPL570, an Affymetrix Human Genome U133 Plus 2.0 array. Gene ontology (GO) analysis was used to identify characteristic the biological attributes of differentially expressed genes (DEGs) between PTC and normal samples. GO annotation was performed on the DEGs obtained, and the process relied on the DAVID online tool. Kyoto Encyclopedia of Genes and Genomes (KEGG) approach enrichment analyses were adopted to obtain the basic functions of the DEGs. The KOBAS online analysis database was used to complete DEG KEGG pathway comparison and analysis. The search tool (STRING) database was mainly used to search for interacting genes and complete the construction of protein-protein interaction (PPI) networks.

Results: Five hundred-ninety DEGs were consistently expressed in the four datasets; 327 of them were upregulated, while 263 were downregulated. Ten DEGs, including five upregulated () and five downregulated () genes, were randomly selected for q-PCR in our own tissue samples to validate the integrated data. The most highly enriched GO terms were extracellular exosome (GO:0070062), cell adhesion (GO:0070062), positive regulation of gene expression (GO:0010628), and extracellular matrix (ECM) organization (GO:0030198). KEGG pathway analysis was performed, and it was found that abnormally expressed genes effectively participated in pathways such as tyrosine metabolism, complement and coagulation cascades, cell adhesion molecules (CAMs), transcriptional misregulation and ECM-receptor interaction pathways.

Conclusions: Five hundred-ninety DEGs were identified in PTC by integrated microarray analysis. The GO and KEGG analyses presented here suggest that the DEGs were enriched in extracellular exosome, tyrosine metabolism, CAMs, complement and coagulation cascades, transcriptional misregulation and ECM-receptor interaction pathways. Functional studies of PTC should focus on these pathways.
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http://dx.doi.org/10.21037/gs-20-673DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944059PMC
February 2021

Waste tire heat treatment to prepare sulfur self-doped char via pyrolysis and KFeO-assisted activation methods.

Waste Manag 2021 Apr 6;125:145-153. Epub 2021 Mar 6.

State Key Laboratory of Coal Combustion, School of Energy and Power Engineering, Huazhong University of Science and Technology, Wuhan 430074, China; China-EU Institute for Clean and Renewable Energy, Huazhong University of Science and Technology, Wuhan 430074, China.

Waste tire was heat-treated to prepare sulfur self-doped chars via pyrolysis and activation processes. Pyrolytic waste tire chars were activated at different temperatures (600 °C, 800 °C, 1000 °C, and 1200 °C) with KFeO additive ratios (mass ratio of KFeO to char) being 0.5, 1, 2, and 3, respectively. The effective activation occurred over 600 °C with KFeO additive ratios over 0.5. The strongest activation occurred at 1000 °C with KFeO additive ratio of 3, and the specific capacitance increased to 129.5 F/g at 1 A/g, which was six times higher than that without KFeO. The activation mechanism revealed that higher KFeO additive ratio promoted the transformation of large aromatic ring systems (≥6 rings) to small ones and smaller pores formation. When KFeO additive ratio was less than 2, high ratio not only promoted alkyl-aryl C-C bonds formation, but also inhibited sulfur enrichment with S 2p (sulphide bridge) converting to S 2p (sulphone bridge). But when the ratio was further increased, slight decomposition of alkyl-aryl C-C bonds with the promoted conversion of S 2p to S 2p was witnessed. Furthermore, higher activation temperature promoted the conversion of aromatic ring systems and alkyl-aryl C-C bonds to form ordered graphitic structures. S 2p was enriched before 800 °C, but both S 2p and S 2p were released at higher temperature. Formation of smaller pores was promoted before 1000 °C, but the char structure was then destroyed to form larger pores when temperature was further increased.
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http://dx.doi.org/10.1016/j.wasman.2021.02.050DOI Listing
April 2021

Improving Bisphosphonate Use and Evaluation in a Rural Family Medicine Practice.

Sr Care Pharm 2021 Mar;36(3):152-158

Objective: To evaluate the prevalence of bisphosphonate use without a drug holiday and to assess the success of an interdisciplinary approach to manage and discontinue bisphosphonate therapy.

Setting: The study was completed at one rural family medicine center.

Practice Description: The practice employs two fulltime pharmacists. Clinical pharmacists' responsibilities include chronic care management as well as collaboration with the team during interdisciplinary clinics. Clinical pharmacists frequently collaborate with other professionals on medication evaluations and quality improvement projects.

Practice Innovation: The pharmacy team and medical resident collaborated to determine appropriateness of bisphosphonate use. This was a two-phase evaluation. In the first phase, therapies were evaluated based on duration and consistency with guideline recommendations based on a retrospective chart review. In the second phase, the pharmacy and medicine team determined if therapy warranted further continuation or if a drug holiday was needed. The team reached out to providers proactively and provided patient and provider education on discontinuing therapy.

Main Outcome Measurements: Patient demographics, bisphosphonate regimen and duration of prescription, frequency of drug holidays, and success rate of therapy discontinuation.

Results: Bisphosphonates were prescribed for an average of 4.2 years with 56 patients prescribed therapy for >5 years. Twenty-one of the 56 patients had a history of a drug holiday, and 13 of the 35 remaining patients had therapy discontinued or a drug holiday was initiated based on the team's recommendation. Patients were more likely to be prescribed therapy for >5 years if they were older and had more provider appointments.
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http://dx.doi.org/10.4140/TCP.n.2021.152DOI Listing
March 2021

Choosing Optimal Microcarcinoma Patients for Active Surveillance Study (Compass): Effectiveness Evaluation of a Preoperative Clinical Framework.

Endocr Pract 2021 Feb 18. Epub 2021 Feb 18.

Department of Head and Neck Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China; Department of Head and Neck Surgery, Fudan University Shanghai Cancer Center, Fudan University, Shanghai, China. Electronic address:

Objective: Active surveillance (AS) is a management alternative for patients with low-risk papillary thyroid microcarcinoma (PTMC). To decide the best candidates for AS, clinicians can use a framework to classify PTMC patients as ideal, appropriate, or inappropriate. This study aimed to explore the correlation between the framework categories and surgical pathology.

Methods: This multicenter retrospective study was conducted between 2014 and 2016. We included 1997 patients who underwent thyroid surgery for the first time due to suspected PTMC and were confirmed as PTMC by postoperative pathology. The consistency of modified preoperative risk stratification and the pathologic condition were evaluated using a consistency ratio and the Kappa coefficient. Stratified analysis was also performed to test consistency in different age groups.

Results: Based on the decision-making framework, 558 (27.9%) patients could receive AS while 810 (40.6%) patients did not require immediate surgery according to the actual postoperative pathology. The sensitivity, false-positive rate, specificity, false-negative rate, and consistency rate were 82.39%, 56.91%, 43.09%, 17.61%, and 66.45%, respectively. The Kappa value was 0.268. Stratified analysis showed that the sensitivity was 87.7% among patients aged 18 to 59 years. In the group aged ≥60 years, the specificity was up to 87.5%, but the sensitivity was low.

Conclusion: The results of the modified risk-stratified clinical decision-making framework did not have a high consistency with the postoperative results. However, the framework showed a good effect in selecting patients for immediate surgery in the younger group and patients for AS in the older group.
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http://dx.doi.org/10.1016/j.eprac.2021.02.008DOI Listing
February 2021

Genome-wide analysis identifies critical DNA methylations within NTRKs genes in colorectal cancer.

J Transl Med 2021 02 16;19(1):73. Epub 2021 Feb 16.

Guangdong Institute of Gastroenterology, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Disease, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Background: Neurotrophic tropomyosin receptor kinases (NTRKs) are a gene family function as oncogene or tumor suppressor gene in distinct cancers. We aimed to investigate the methylation and expression profiles and prognostic value of NTRKs gene in colorectal cancer (CRC).

Methods: An analysis of DNA methylation and expression profiles in CRC patients was performed to explore the critical methylations within NTRKs genes. The methylation marker was validated in a retrospectively collected cohort of 229 CRC patients and tested in other tumor types from TCGA. DNA methylation status was determined by quantitative methylation-specific PCR (QMSP).

Results: The profiles in six CRC cohorts showed that NTRKs gene promoter was more frequently methylated in CRC compared to normal mucosa, which was associated with suppressed gene expression. We identified a specific methylated region within NTRK3 promoter targeted by cg27034819 and cg11525479 that best predicted survival outcome in CRC. NTRK3 promoter methylation showed independently predictive value for survival outcome in the validation cohort (P = 0.004, HR 2.688, 95% CI [1.355, 5.333]). Based on this, a nomogram predicting survival outcome was developed with a C-index of 0.705. Furthermore, the addition of NTRK3 promoter methylation improved the performance of currently-used prognostic model (AIC: 516.49 vs 513.91; LR: 39.06 vs 43.64, P = 0.032). Finally, NTRK3 promoter methylation also predicted survival in other tumors, including pancreatic cancer, glioblastoma and stomach adenocarcinoma.

Conclusions: This study highlights the essential value of NTRK3 methylation in prognostic evaluation and the potential to improve current prognostic models in CRC and other tumors.
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http://dx.doi.org/10.1186/s12967-021-02740-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7885252PMC
February 2021

AIEgen modulated per-functionalized flower-like IRMOF-3 frameworks with tunable light emission and excellent sensing properties.

Chem Commun (Camb) 2021 Mar 5;57(19):2392-2395. Epub 2021 Feb 5.

College of Chemistry and Chemical Engineering, The Hunan Provincial Key Laboratory of Water Environment and Agriculture Product Safety, Central South University, Changsha 410083, Hunan, China.

A series of functional IRMOF-3 frameworks with solid-state luminescence and tuneable light emission (from 490 to 608 nm) have been synthesized by per-functionalizing AIE-active Schiff-bases with zinc. These precursor AIE-active ligands endowed the functional frameworks with boosted fluorescence emission efficiencies (from 0.16% to 1.03%). IRMOF-3-h revealed a flower-like morphology attributed to the formation of J-aggregates, and could be used as a fluorescent probe for sensitive detection of copper(ii) (135 pM) and thiols (subnanomole).
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http://dx.doi.org/10.1039/d0cc08403dDOI Listing
March 2021

Low-Power Near-Infrared-Responsive Upconversion Nanovectors.

ACS Appl Mater Interfaces 2021 Feb 1;13(6):7094-7101. Epub 2021 Feb 1.

College of Biomass Science and Engineering, Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, P.R. China.

Activating upconversion nanoparticle-based photoresponsive nanovectors (UCPNVs) by upconversion visible light at low-power near-infrared (NIR) excitation can realize deeper biotissue stimulation with a minimized overheating effect and photodamage. Here, we demonstrate a facile strategy to construct new surface-decorated UCPNVs based on Passerini three-component reaction (P-3CR) in highly convenient and effective manners. Such UCPNVs materials have a tailored deprotecting wavelength that overlaps upconversion blue light. By using 3-perylenecarboxaldehyde, Tm/Yb ion-doped UCNP-containing isocyanides, and antitumor agent chlorambucil as the three components, the resulting monodisperse UCPNV exhibits an efficient release of caged chlorambucil under a very low 976 nm power. This approach expands the synthetic toolbox to enable quick development of UCPNVs for UCNP-assisted low-power NIR photochemistry.
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http://dx.doi.org/10.1021/acsami.0c21115DOI Listing
February 2021

The complete chloroplast genome sequence of (Blechnaceae).

Mitochondrial DNA B Resour 2020 May 12;5(3):2034-2035. Epub 2020 May 12.

Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang Normal University, Huanggang, China.

(Hooker) J. Smith, a member of Blechnaceae, is a rare and endangered species in tropical Asia. Here we assembled and annotated the complete chloroplast (cp) genome. It is 149,730 bp in length and encodes 88 protein-coding genes, 36 transfer RNA (tRNA) genes and eight ribosomal RNA (rRNA) genes. This chloroplast genome sequencing offers a useful resource for future conservation genetics and phylogenetic studies.
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http://dx.doi.org/10.1080/23802359.2020.1756959DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782652PMC
May 2020

Evaluating the impact of rehabilitation nursing intervention on quality of life in patients with burn injuries: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Jan;100(1):e23879

Department of Burns, Tongren Hospital of Wuhan University & Wuhan Third Hospital, Wuhan, Hubei, P. R. China.

Background: Despite the availability of pharmacological intervention, patients with burn injuries experience pain during the treatment of wounds. Supplementary rehabilitation nursing intervention are required to enhance the wellbeing of patients sustaining injuries from burns. The present study aims to conduct a systematic exploration of the impact of rehabilitation nursing intervention on the wellbeing in patients sustaining burn injuries.

Methods: The electronic databases listed below will be searched systematically: PubMed, EMBASE, CINAHL, Cochrane Library, Web of Science, Scopus, China National Knowledge Infrastructure, and WanFang database. All the databases will be searched from their inauguration to November 2020. There will be no language constraints. Independent undertaking by 2 authors will select studies, extract data from selected studies, and assess the quality of the included studies. All disagreements will be resolved through discussion, or by consulting a third independent author. This study will make use of RevMan 5.3 software to perform statistical analysis.

Results: The present protocol summarizes high-quality evidence to assess the impact of rehabilitation nursing intervention on the wellbeing of patients sustaining burn injuries.

Conclusion: The results of the present protocol has the potential to present evidence to assess whether rehabilitation nursing intervention can enhance the wellbeing of patients sustaining burn injuries.

Registration Number: November 17, 2020.osf.io/t6b8c/. (https://osf.io/t6b8c/).
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http://dx.doi.org/10.1097/MD.0000000000023879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793314PMC
January 2021

Fabric Retrieval Based on Multi-Task Learning.

IEEE Trans Image Process 2021 11;30:1570-1582. Epub 2021 Jan 11.

Due to the potential values in many areas such as e-commerce and inventory management, fabric image retrieval, which is a special case in Content Based Image Retrieval (CBIR), has recently become a research hotspot. It is also a challenging issue with serval obstacles: variety and complexity of fabric appearance, high requirements for retrieval accuracy. To address this issue, this paper proposes a novel approach for fabric image retrieval based on multi-task learning and deep hashing. According to the cognitive system of fabric, a multi-classification-task learning model with uncertainty loss and constraint is presented to learn fabric image representation. Then we adopt an unsupervised deep network to encode the extracted features into 128-bits hashing codes. Further, the hashing codes are regarded as the index of fabrics image for image retrieval. To evaluate the proposed approach, we expanded and upgraded the dataset WFID, which was built in our previous research specifically for fabric image retrieval. The experimental results show that the proposed approach outperforms the state-of-the-art.
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http://dx.doi.org/10.1109/TIP.2020.3043877DOI Listing
January 2021

Complete chloroplast genome sequence and phylogenetic analysis of , a highly ornamental species endemic in central China.

Mitochondrial DNA B Resour 2020 Jan 20;5(1):720-722. Epub 2020 Jan 20.

Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang Normal University, Huanggang, China.

Z. Z. Zhao et Z. W. Xie is a species with high horticultural and medicinal value, which found only in Dabie Mountain of central China. In this study, we sequenced, assembled and annotated the complete chloroplast (cp) genome of . The length of complete cp genome was 160,104 bp, with a typical quadripartite structure comprising a pair of inverted repeat regions (IRs; 26,591 bp), a large single copy region (LSC; 88,110 bp) and a small single copy region (SSC; 18,812 bp). The whole cp genome contained 129 unique genes, including 79 protein-coding genes (PCGs), 30 transfer RNA (tRNA) genes and four ribosomal RNA (rRNA) genes. The maximum-likelihood (ML) phylogenetic analysis indicated that was relatively closed to the . This chloroplast genome would provide a valuable genetic resource for .
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http://dx.doi.org/10.1080/23802359.2020.1714511DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7748855PMC
January 2020

Improving Chronic Obstructive Pulmonary Disease (COPD) Symptoms Using a Team-Based Approach.

J Am Board Fam Med 2020 Nov-Dec;33(6):978-985

From the Department of Family Medicine, West Virginia University School of Medicine, Robert C. Byrd Health Sciences Center, Morgantown (SP, MMA, AMA, JX, DEK); School of Pharmacy, West Virginia University School of Medicine, Robert C. Byrd Health Sciences Center, Morgantown (MMA, BS).

Background: Chronic obstructive pulmonary disease (COPD) is the third leading cause of death in the United States (US), with West Virginia bearing a disproportionate disease burden. Complex COPD cases can be difficult to manage during a standard primary care provider (PCP) visit, and pharmacological treatment regimens should be individually tailored to each patient.

Methods: To address these needs, the West Virginia University Department of Family Medicine created an interdisciplinary COPD specialty clinic that uses a team-based approach to treat patients with COPD. In order to evaluate the effectiveness of the specialty clinic, we conducted a retrospective chart review to examine the impact of the clinic on patient hospitalizations, emergency department visits, and urgent care visits six months and one year before and after initiating care at the clinic. We also examined the impact of the clinic on patients' self-reported nicotine dependency, COPD symptoms, and tobacco use behavior. Patients referred to the clinic and having at least one visit from February 2015 to February 2019 were included in this study (n = 149).

Results: Patients treated at the COPD specialty clinic had significantly fewer hospital admissions and ED visits six months after and one year after initiating care at the clinic as compared to six months before and one year before, respectively. Patients at this clinic also reported smoking significantly fewer cigarettes per day with significantly fewer self-identifying as smokers and experiencing significantly reduced COPD symptoms.

Conclusion: An interdisciplinary, team-based approach was effective for improving the health of COPD patients in an Appalachian academic primary care practice.
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http://dx.doi.org/10.3122/jabfm.2020.06.200176DOI Listing
April 2020

Glucosamine/Chondroitin and Mortality in a US NHANES Cohort.

J Am Board Fam Med 2020 Nov-Dec;33(6):842-847

From the Department of Family Medicine, West Virginia University, Morgantown.

Background: Limited previous studies in the United Kingdom or a single US state have demonstrated an association between intake of glucosamine/chondroitin and mortality. This study sought to investigate the association between regular consumption of glucosamine/chondroitin and overall and cardiovascular (CVD) mortality in a national sample of US adults.

Methods: Combined data from 16,686 participants in National Health and Nutrition Examination Survey 1999 to 2010, merged with the 2015 Public-use Linked Mortality File. Cox proportional hazards models were conducted for both CVD and all-cause mortality.

Results: In the study sample, there were 658 (3.94%) participants who had been taking glucosamine/chondroitin for a year or longer. During followup (median, 107 months), there were 3366 total deaths (20.17%); 674 (20.02%) were due to CVD. Respondents taking glucosamine/chondroitin were less likely to have CVD mortality (hazard ratio [HR] = 0.51; 95% CI, 0.28-0.92). After controlling for age, use was associated with a 39% reduction in all-cause (HR = 0.61; 95% CI, 0.49-0.77) and 65% reduction (HR = 0.35; 95% CI, 0.20-0.61) in CVD mortality. Multivariable-adjusted HR showed that the association was maintained after adjustment for age, sex, race, education, smoking status, and physical activity (all-cause mortality, HR = 0.73; 95% CI, 0.57-0.93; CVD mortality, HR = 0.42; 95% CI, 0.23-0.75).

Conclusions: Regular intake of glucosamine/chondroitin is associated with lower all-cause and CVD mortality in a national US cohort and the findings are consistent with previous studies in other populations. Prospective studies to confirm the link may be warranted.
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http://dx.doi.org/10.3122/jabfm.2020.06.200110DOI Listing
May 2020

A novel three-dimensional FeSnC/C hybrid nanofiber absorber for lightweight and highly-efficient microwave absorption.

Phys Chem Chem Phys 2020 Nov;22(45):26104-26108

School of Science, Jiangsu University of Science and Technology, Zhenjiang 212003, China.

3D Fe3SnC/C hybrid nanofibers are proposed as a novel high-performance microwave absorber. At only 20 wt% filler loading, the optimal reflection loss reaches -119.2 dB at 17.1 GHz and the effective absorption bandwidth is 7.4 GHz with a thickness of 2.3 mm, outperforming most of the reported absorbers.
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http://dx.doi.org/10.1039/d0cp04594bDOI Listing
November 2020

Sustainable Advanced Fenton-like Catalysts Based on Mussel-Inspired Magnetic Cellulose Nanocomposites to Effectively Remove Organic Dyes and Antibiotics.

ACS Appl Mater Interfaces 2020 Nov 10;12(46):51952-51959. Epub 2020 Nov 10.

Beijing Key Laboratory of Ionic Liquids Clean Process, CAS Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.

The development of biocompatible advanced Fenton-like catalysts with high catalytic activity, good stability, and recyclability using sustainable biosourced materials is of considerable interest yet remains a challenge. Herein, we develop a novel mussel-inspired magnetic cellulose nanocomposite (MCNF/PDA) with carboxylated cellulose nanofibers (CNF) and explore as advanced Fenton-like catalysts to effectively degrade organic dyes and antibiotics. The MCNF/PDA nanocomposites were prepared by anchoring FeO nanoparticles to CNFs via chemical deposition followed with PDA coatings. The composites exhibit an excellent degradation activity toward methylene blue (MB) in a wide pH range of 2-10 in the presence of HO and have a maximum degradation capacity of 2265 mg/g. Moreover, the MCNF/PDA nanocatalysts are highly stable and can be easily regenerated. After four cycles, it can still achieve the removal rate as high as 95%. In addition, the MCNF/PDA nanocatalysts also demonstrate an excellent degradation performance to the antibiotic tetracycline. This work provides new insights into fabricating biocompatible cellulosic-based advanced Fenton catalysts with sustainable biomass-derived materials to efficiently remove organic pollutants from wastewater.
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http://dx.doi.org/10.1021/acsami.0c14820DOI Listing
November 2020

Reconstruction of the ocular surface by autologous transplantation of rabbit adipose tissue-derived stem cells on amniotic membrane.

Ann Transl Med 2020 Sep;8(17):1062

Department of Ophthalmology, Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, China.

Background: Corneal disease is the second most common cause of blindness in China. Clinically, treatment options for corneal diseases with limbal stem cell deficiency (LSCD) are limited due to a shortage of organ donors and inevitable immune rejection. This study aims to determine the efficacy of reconstructing the ocular surface using autologous cultivated adipose tissue-derived stem cells (ADSCs) and to develop a new clinical treatment for patients with LSCD.

Methods: A rabbit LSCD model was first established. Two weeks later, the animals were divided into three groups, including the sham group, the amniotic membrane transplantation group, and the ADSC combined with amniotic membrane transplantation group, and underwent surgery. The efficacy of reconstructing the ocular surface using ADSCs was evaluated using immunofluorescent staining, confocal microscopy (CM) observation, H&E staining, immunohistochemical staining, and scanning transmission electron microscopy observation one, two and four weeks after surgery.

Results: Evaluations of immunofluorescent staining of the cornea pre- and post-surgery yielded significantly lower scores for the corneas in the ADSCs transplantation group than for those in the sham group (F=-7, P=0.002, <0.05) and the amniotic membrane transplantation group (F=-4.67, P=0.018, <0.05) two weeks after surgery. Four weeks after surgery, the corneas of the ADSC combined with amniotic membrane transplantation group were scored significantly lower than those in the sham group (F=-8, P=0.007, <0.05) and the amniotic membrane transplantation group (F=-5.33, P=0.046, <0.05). The data suggest that the use of ADSCs to treat LSCD showed greater efficacy than the other treatment methods. The growth of ADSCs on the corneal surface was examined using confocal and electron microscopes. K3/K12 expression in the corneal epithelium, which was reconstructed by ADSCs, was negative, as shown by immunohistochemical staining.

Conclusions: Ocular surface reconstruction can be improved by using ADSCs as seed cells and the amniotic membrane as a carrier, thus providing a new therapeutic strategy for patients with LSCD.
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http://dx.doi.org/10.21037/atm-20-4368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7575941PMC
September 2020

Gut microbiota modulation and anti-inflammatory properties of Xuanbai Chengqi decoction in septic rats.

J Ethnopharmacol 2021 Mar 1;267:113534. Epub 2020 Nov 1.

Department of Emergency Medicine, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, PR China. Electronic address:

Ethnopharmacological Relevance: Xuanbai Chengqi decoction (XBCQ), a traditional Chinese medicine formulation, was reported to have a protective role in a variety of pulmonary infection diseases. However, its mechanism remains uncertain. In the current study, we investigated the potential mechanism of XBCQ, its therapeutic effects on organ injuries induced by sepsis and gut microbiota modulation.

Material And Methods: 80 Male Sprague Dawley rats were performed cecal ligation and puncture (CLP) for sepsis model and 60 of them were treated with different doses of XBCQ (3.78, 7.56, 15.12 g/Kg, 20 rats per group) twice per day. After the most valid dose was determined, another 40 rats were divided randomly into four groups: sham group, sham + XBCQ group, sepsis group, sepsis + XBCQ group. The sepsis + XBCQ group was treated with XBCQ by intragastric administration and then twice per day. Feces of the rats were collected and the gut microbiota constituents were analyzed by 16S rDNA sequencing. Histological changes were observed by H&E staining. Occludin content in the colon was determined by immunohistochemical analysis. The concentrations of cytokines were determined by enzyme-linked immunosorbent assay (ELISA) kits.

Results: The survival rate of septic rats was increased significantly at the dose of 7.56 g/Kg from 50% to 80% at 72 h. The gut microbiota richness and composition were disturbed in septic rats. XBCQ altered the gut microbiota, involving alpha diversity changes, significantly reducing the relative abundance of Bacteroidaceae and ClostridiumXI and increasing that of Firmicutes and Actinobacteria. Furthermore, the relative abundances of Lactobacillus, Butyricicoccus and Bifidobacterium were increased by XBCQ. Moreover, the gut barrier dysfunction was improved by XBCQ through restoring the impaired tight conjunction protein Occludin. The concentration of diamine oxidase was decreased, while the D-lactate level was elevated. Meanwhile, the level of myeloperoxidase (MPO) in the lung tissue of the XBCQ-treated group was reduced. Lung injury was also alleviated by decreased levels of tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β) and interleukin 10 (IL-10) in bronchoalveolar lavage fluids (BALFs). The relative abundance of potential microbial biomarkers in four groups significantly correlated with the concentration of inflammatory factors in BALFs.

Conclusions: Our results suggested that XBCQ had a protective role against sepsis by modulating the gut microbiota, restoring the intestinal epithelial barrier and decreasing inflammatory responses.
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http://dx.doi.org/10.1016/j.jep.2020.113534DOI Listing
March 2021

The correlation between plasma total homocysteine level and gestational diabetes mellitus in a Chinese Han population.

Sci Rep 2020 10 29;10(1):18679. Epub 2020 Oct 29.

Department of Maternal and Child Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

To assess the correlation between plasma total homocysteine (tHcy) level and gestational diabetes mellitus (GDM) in a Chinese Han population. This case-control study included 350 GDM patients and 346 gestational week-matched normal glucose tolerance (NGT) pregnant women. Plasma tHcy and insulin levels were analyzed by HPLC and ELISA respectively. Logistic regression analysis was used to investigate the correlation between plasma tHcy level and risk of GDM. Women with GDM had a higher plasma tHcy level than NGT women (6.61 ± 1.32 vs. 6.17 ± 1.29 μmol/L, P = 0.001)). The GDM risk was 1.79 (OR = 1.79, 95% CI 1.18-2.72, P = 0.006) times higher in women whose plasma tHcy level was ≥ 7.29 μmol/L compared to women with plasma tHcy level < 5.75 μmol/L. Stratified analysis showed the GDM risk were much higher when HOMA-IR index ≥ 2 (OR = 5.42, 95% CI 2.51-11.74, P < 0.001), age ≥ 30 years (OR = 5.14, 95% CI 2.78-9.52, P < 0.001), or women with a family history of type 2 diabetes mellitus (T2DM) (OR = 4.13, 95% CI 1.78-9.56, P = 0.001). In the Chinese Han population, an elevated plasma tHcy level may increase the overall risk of GDM especially in women with a high HOMA-IR index, increasing age or with family history of T2DM.
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http://dx.doi.org/10.1038/s41598-020-75797-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7596467PMC
October 2020

Survey report on keratoplasty in China: A 5-year review from 2014 to 2018.

PLoS One 2020 15;15(10):e0239939. Epub 2020 Oct 15.

Corneal Disease Group of Ophthalmological Society of Chinese Medical Association (China Cornea Society), Jinan, China.

To provide the general information on corneal transplantation (CT) in China, China Cornea Society designed a questionnaire on CT from 2014 to 2018 and entrusted it to 31 committee members for implementation of the survey nationwide. This article presents the results of the survey and compares the indicators used in the survey and those in the annual statistical report released by the Eye Bank Association of America (EBAA). The number of corneal transplantations completed by the 64 hospitals from 2014 to 2018 was respectively 5377, 6394, 7595, 8270 and 8980, totally 36,616 (22,959 male and 13,657 female). The five largest hospitals by the number of corneal transplantations completed 15,994 surgeries in total, accounting for 43.68% of all the surgeries performed in the 64 hospitals. The most common indication for corneal transplantations was corneal leukoma (7683, 20.98%), followed by bacterial keratitis (4209, 11.49%), corneal dystrophies (4189, 11.44%), keratoconus (3578, 9.77%) and corneal perforation (2839, 7.75%). The main surgical techniques were penetrating keratoplasty (PK) (19,896, 54.34%), anterior lamellar keratoplasty (ALK) (13,869, 37.88%). The proportion of PK decreased from 57.97% in 2014 to 52.88% in 2018 while the proportion of ALK increased from 36.04% in 2014 to 37.92% in 2018. The geographical distribution of keratoplasties performed in China is unbalanced. PK and ALK were the main techniques of CT and corneal leukoma, bacterial keratitis and corneal dystrophies were the main indications for CT in China.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0239939PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7561196PMC
November 2020

Research on treatment and mechanism of salicylhydroxamic acid flotation wastewater by O-BAF process.

Water Sci Technol 2020 Sep;82(5):861-876

School of Chemical and Environmental Engineering, China University of Mining & Technology, Beijing, China E-mail:

Salicylhydroxamic acid is an effective and selective collector for tungsten and molybdenum ores. However, the salicylhydroxamic acid flotation wastewater discharge may cause damage to the water environment for the residual processing reagents with poor biodegradability. Combined O and biological aerated filter (BAF) has a well-known potential for removing refractory or toxic organic pollutants. Combined process of O and BAF (O-BAF) was applied to treat the simulated wastewater from W-Mo mineral processing in this study. Compared single ozonation to O-BAF, various influencing factors were discussed like O dosage, reaction time, initial pH value, gas-water ratio and organic loading. Meanwhile, degradation mechanism of salicylhydroxamic acid was reduced. Under the optimal experiment conditions as pH value 8, O dosage 1.3 mg·L, reaction time 15 min, the five-day biochemical oxygen demand (BOD)/chemical oxygen demand of potassium dichromate (CODCr) value increased to from 0.19 to 0.35. The effluent was pumped to the following BAF process, when the optimal experiment conditions was organic load = 0.82 kgCODCr (m·d), gas-water ratio = 6:1, CODCr concentration of effluent was 28.92 mg·L and the removal ratio was 86.26%, while the removal ratio could higher to 91.12% for the O-BAF combined process. The effluent could meet the discharge and reuse emission standards requirements in China. UV-vis absorption spectra and high performance liquid chromatography showed the degradation pathway of salicylhydroxamic acid by ozone oxidation was salicylhydroxamic acid → salicylic acid + hydroxylamine → catechol → maleic → small molecular organic acid → carbon dioxide + water.
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http://dx.doi.org/10.2166/wst.2020.388DOI Listing
September 2020

When EGFR inhibitor meets autoimmune disease: Severe corneal complications in a patient with Sjögren syndrome after erlotinib treatment.

Eur J Ophthalmol 2020 Sep 21:1120672120958300. Epub 2020 Sep 21.

Department of Ophthalmology and Visual Science, Eye, Ear, Nose and Throat Hospital, Shanghai Medical College of Fudan University, Shanghai, China.

Introduction: To report the first case of severe corneal complications in a patient with Sjögren syndrome after receiving erlotinib treatment.

Case Description: A 51-year-old woman with Sjögren syndrome presented with persistent corneal epithelial defects, which did not respond to conservative therapies. She had been diagnosed with lung cancer and was being treated with erlotinib, a kind of epidermal growth factor receptor (EGFR) inhibitor, for over 2 years. Cornea stromal melting and perforation were not avoided and a total of four penetrating keratoplasties were performed. Stable corneal surface was achieved after the erlotinib treatment was paused.

Conclusion: This report, to the best of our knowledge, is the first description of severe ocular complications present in a patient with Sjögren syndrome after receiving the EGFR inhibitor. The underlying ocular or system diseases that were thought to be irrelevant upon receiving the EGFR inhibitors might negatively influence the tumor patients planning to take these kinds of targeted medication. Therefore, it is important to have eye examinations before and during the EGFR inhibitors treatment and supplement the relative contraindications (such as Sjögren syndrome) to EGFR inhibitor treatments as necessary.
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http://dx.doi.org/10.1177/1120672120958300DOI Listing
September 2020

Calcitriol inhibits apoptosis via activation of autophagy in hyperosmotic stress stimulated corneal epithelial cells in vivo and in vitro.

Exp Eye Res 2020 11 5;200:108210. Epub 2020 Sep 5.

Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, China; NHC Key Laboratory of Myopia, Fudan University, Shanghai, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China. Electronic address:

Background: Previously, calcitriol has been demonstrated as a potential therapeutic agent for dry eye, whilst its role on corneal epithelium death remains unclear. This study aims to investigate the relationship between apoptosis and autophagy on dry eye related scenario, as well as the effect of calcitriol and its potential mechanism.

Methods: In vitro, immortalized human corneal epithelial cells (iHCEC) were cultured in hyperosmotic medium with or without various concentrations of calcitriol and other reagents. In vivo, Wistar rats were applied with benzalkonium chloride to induce dry eye. Then rats were topically treated with calcitriol (10 M) for 14 days. Autophagy flux (LC3B-II and SQSTM1/P62) was examined by western blotting or immunostaining. To test cell apoptosis, western blotting for cleaved caspase-3, Annexin V/PI double staining and TUNEL assay were used. CCK-8 assay was performed to detect the cell viability. Small interfering RNA was used to knock down the expression of vitamin D receptor in iHCECs.

Results: Autophagy activation could protect iHCECs against HS induced apoptosis in vitro, and calcitriol was able to augment autophagy flux via VDR signaling, shown as the remarkably elevated expression of LC3B-II, as well as the declined p62 expression. In vivo results further supported the protective role of calcitriol on corneal epithelium apoptosis through promoting autophagy in dry eye rats.

Conclusion: The current study indicated that autophagy was an adaptive change of corneal epithelial cells in response to hyperosmotic stress and calcitriol could prevent cells from apoptosis via further activation of autophagy through VDR pathway.
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http://dx.doi.org/10.1016/j.exer.2020.108210DOI Listing
November 2020