Publications by authors named "Jun Wang"

9,685 Publications

  • Page 1 of 1

Self-Distilled Supervised Contrastive Learning for diagnosis of breast cancers with histopathological images.

Comput Biol Med 2022 May 17;146:105641. Epub 2022 May 17.

Key Laboratory of Specialty Fiber Optics and Optical Access Networks, Joint International Research Laboratory of Specialty Fiber Optics and Advanced Communication, School of Communication and Information Engineering, Shanghai University, China; Shanghai Institute for Advanced Communication and Data Science, Shanghai University, China. Electronic address:

Convolutional neural networks (CNN) and its variants have been widely used for developing the histopathological image based computer-aided diagnosis (CAD). However, the annotated data are scarce in clinical practice, and limited training samples generally cannot well train the CNN model, resulting in degraded predictive performance. To this end, we propose a novel Self-Distilled Supervised Contrastive Learning (SDSCL) algorithm to improve the diagnostic performance of a CNN-based CAD for breast cancer. In particular, the original histopathological images are first decomposed into H and E stain views, which are served as the augmented sample pairs in the supervised contrastive learning (SCL). Due to the complementary characteristic of the two stain views, this data-driven SCL guide the CNN model to efficiently learn the discriminative features, alleviating the problem of small sample size. Furthermore, self-distillation is embedded into the SCL framework, in which the CNN model jointly distills itself and conducts SCL to further improve feature representation. The proposed SDSCL is evaluated on two public breast histopathological datasets, which outperforms all the compared algorithms. Its average classification accuracy, precision, recall, and F1 scores are 94.28%, 94.64%, 94.58%, 94.34%, respectively, on Bioimaging dataset, and 80.44%, 81.92%, 80.57%, 80.10% on Databiox dataset. The experimental results on two datasets indicate that SDSCL has the potential for the histopathological image based CAD.
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http://dx.doi.org/10.1016/j.compbiomed.2022.105641DOI Listing
May 2022

The CD8α-PILRα interaction maintains CD8 T cell quiescence.

Science 2022 May 26;376(6596):996-1001. Epub 2022 May 26.

Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.

T cell quiescence is essential for maintaining a broad repertoire against a large pool of diverse antigens from microbes and tumors, but the underlying molecular mechanisms remain largely unknown. We show here that CD8α is critical for the maintenance of CD8 T cells in a physiologically quiescent state in peripheral lymphoid organs. Upon inducible deletion of CD8α, both naïve and memory CD8 T cells spontaneously acquired activation phenotypes and subsequently died without exposure to specific antigens. PILRα was identified as a ligand for CD8α in both mice and humans, and disruption of this interaction was able to break CD8 T cell quiescence. Thus, peripheral T cell pool size is actively maintained by the CD8α-PILRα interaction in the absence of antigen exposure.
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http://dx.doi.org/10.1126/science.aaz8658DOI Listing
May 2022

Functional Analysis of Serum Long Noncoding RNAs in Patients with Atrial Fibrillation.

Dis Markers 2022 16;2022:2799123. Epub 2022 May 16.

Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Objectives: Long noncoding RNAs (lncRNAs) are closely related to diverse diseases. However, its role in atrial fibrillation (AF) pathogenesis needs further exploration.

Design: We performed microarray analysis on the serum samples from 70 healthy volunteers and 70 AF patients. This study was aimed at detecting the levels of serum lncRNAs and mRNAs and bioinformatically analyze them to establish potential marker(s) for AF diagnosis. Receiver operating curve (ROC) and area under the curve (AUC) were employed to address the AF diagnostic power of lncRNAs.

Results: In the AF serum samples, 753 lncRNAs and 802 mRNAs ( ≤ 0.05; fold change ≥ 2) were upregulated, and 315 lncRNAs and 153 mRNAs were downregulated, as opposed to healthy serum samples. Using bioinformatic analysis, we analyzed the top 4 differentially expressed (DE) lncRNAs, namely, , , , and , and found that the PI3K-AKT cell proliferation signaling pathway was most affected. This was in accordance with our functional analysis of DE mRNAs and adjacent lncRNAs. Notably, the elevated serum levels were strongly associated with AF incidence.

Conclusions: Our work highlights the role of lncRNAs in AF pathogenesis and provides a novel serum biomarker for AF diagnosis.
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http://dx.doi.org/10.1155/2022/2799123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9126683PMC
May 2022

Clinical analysis of subxiphoid single-port thoracoscopic surgery for simultaneous bilateral lung lesion resection.

BMC Surg 2022 May 25;22(1):203. Epub 2022 May 25.

Department of Thoracic Surgery, The First Affiliated Hospital of University of Science and Technology of China, Hefei, 230001, People's Republic of China.

Objective: To investigate the efficacy and safety of simultaneous subxiphoid single-port thoracoscopic resection of bilateral lung lesions.

Methods: This retrospective study analyzed the clinical data of 72 patients who underwent resection of bilateral lung lesions at the Department of Thoracic Surgery in the First Affiliated Hospital of University of Science and Technology of China between August 2020 and January 2022. Surgery-related parameters were compared between patients who underwent subxiphoid single-port thoracoscopy (subxiphoid group, 36 patients) and patients who underwent intercostal single-port thoracoscopy (intercostal group, 36 patients).

Results: Compared to the intercostal group, the subxiphoid group exhibited significantly better postoperative catheterization time (P = 0.013), postoperative thoracic drainage, postoperative visual analog scale pain scores at 24 and 48 h, and incision pain and numbness at 1 and 3 months after surgery (all P < 0.05). There were no significant differences in operation time, intraoperative blood loss, or postoperative complications between the two groups (all P > 0.05). There were no cases of perioperative mortality, conversion to thoracotomy, or serious complications in either group.

Conclusion: Subxiphoid single-port thoracoscopic surgery for simultaneous resection of bilateral lung lesions is safe and effective, reduces postoperative acute and chronic pain, decreases trauma, allows faster recovery, and is more consistent with the concept of minimally invasive surgery than bilateral intercostal single-port thoracoscopy. Thus, this subxiphoid single-port thoracoscopic surgery approach should be considered for clinical application.
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http://dx.doi.org/10.1186/s12893-022-01646-4DOI Listing
May 2022

Mitochondrial damage-associated molecular patterns trigger arginase-dependent lymphocyte immunoregulation.

Cell Rep 2022 May;39(8):110847

Department of Pathology, Dalhousie University, Halifax, NS, Canada; Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada; Beatrice Hunter Cancer Research Institute, Halifax, NS, Canada; Canadian Center for Vaccinology, IWK Health Centre, Halifax, NS, Canada. Electronic address:

Tissue damage leads to loss of cellular and mitochondrial membrane integrity and release of damage-associated molecular patterns, including those of mitochondrial origin (mitoDAMPs). Here, we describe the lymphocyte response to mitoDAMPs. Using primary cells from mice and human donors, we demonstrate that natural killer (NK) cells and T cells adopt regulatory phenotypes and functions in response to mitoDAMPs. NK cell-mediated cytotoxicity, interferon gamma (IFN-γ) production, T cell proliferation, and in vivo anti-viral T cell activation are all interrupted in the presence of mitoDAMPs or mitoDAMP-rich irradiated cells in in vitro and in vivo assays. Mass spectrometry analysis of mitoDAMPs demonstrates that arginase and products of its enzymatic activity are prevalent in mitoDAMP preparations. Functional validation by arginase inhibition and/or arginine add-back shows that arginine depletion is responsible for the alteration in immunologic polarity. We conclude that lymphocyte responses to mitoDAMPs reflect a highly conserved mechanism that regulates inflammation in response to tissue injury.
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http://dx.doi.org/10.1016/j.celrep.2022.110847DOI Listing
May 2022

Nkx2.8 promotes chemosensitivity in bladder urothelial carcinoma via transcriptional repression of MDR1.

Cell Death Dis 2022 May 24;13(5):492. Epub 2022 May 24.

Department of Urology, Sun Yat-Sen University Cancer Center, Guangzhou, PR China.

Multidrug resistance gene 1 (MDR1), a key factor contributing to drug insensitivity, has been associated with treatment failure and poor prognoses in various cancers, including bladder urothelial carcinoma (UC). Here we show that positive Nkx2.8 expression was associated with better prognosis of UC patients received chemotherapy. Patients with positive Nkx2.8 expression had promising prognosis from adjuvant chemotherapy. Enforced expression of Nkx2.8 promotes drug sensitivity of UC cells. Mechanistic investigations showed that Nkx2.8 negatively regulated expression of MDR1 by binds directly to the MDR1 promoter and transcriptionally represses MDR1 expression. P-gp inhibitor reversed chemosensitivity inhibition by Nkx2.8 scilencing. In clinical UC specimens, expression of Nkx2.8 inversely correlated with P-gp expression, and UC patients with Nkx2.8 positivity and low P-gp expression displayed the best prognosis. Our findings uncovered a new mechanism of chemosensitivity in UC cells and proposing Nkx2.8-MDR1 axis as a novel candidate target for therapeutic intervention of UC.
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http://dx.doi.org/10.1038/s41419-022-04947-xDOI Listing
May 2022

Design, synthesis and biological evaluation of novel pleuromutilin derivatives possessing 4-aminothiophenol linker as promising antibacterial agents.

Bioorg Chem 2022 May 10;126:105859. Epub 2022 May 10.

Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China. Electronic address:

A series of novel pleuromutilin derivatives containing 4-aminothiophenol moieties have been designed and synthesized as promising antibacterial agents against Methicillin-resistant Staphylococcus aureus (MRSA). The in vitro antibacterial activity of these semisynthetic derivatives against 4 strains of S. aureus (MRSA ATCC 43300, S. aureus ATCC 29213, S. aureus 144 and S. aureus AD3) was evaluated by the broth dilution method. Most of the synthesized derivatives displayed prominent in vitro activity (MIC ≤ 0.5 µg/mL). 12 Compounds possessed superior antibacterial activity against MRSA compared with valnemulin and retapamulin (MIC = 0.0625 µg/mL). Compounds 12, 16a, 16c and 19 exhibited the most effective antibacterial effect against MRSA (MIC = 0.015 µg/mL). Furthermore, the time-kill curves showed compounds 12 and 19 had a certain inhibitory effect against MRSA in vitro. Compounds 12 and 19 possessed longer PAE time (2.74 h and 3.11 h, respectively) than tiamulin (PAE = 2.04 h) against MRSA after exposure at 4 × MIC concentration for 2 h. Compounds 12 and 19 also displayed superior in vivo antibacterial efficacy (-1.20 log CFU/mL and -1.21 log CFU/mL, respectively) than tiamulin (-0.75 log CFU/mL) in reducing MRSA load in the mice thigh infection model. In addition, compound 19 had barely inhibitory effect on RAW 264.7 and 16HBE cells at 8 µg/mL. In molecular docking study, upon docking into the 50S ribosomal subunit, the binding free energy (ΔG) of compound 12 and 19 was calculated to be -9.02 kcal/mol and -9.89 kcal/mol, respectively.
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http://dx.doi.org/10.1016/j.bioorg.2022.105859DOI Listing
May 2022

Di-(2-ethylhexyl) phthalate exacerbated the toxicity of polystyrene nanoplastics through histological damage and intestinal microbiota dysbiosis in freshwater Micropterus salmoides.

Water Res 2022 May 16;219:118608. Epub 2022 May 16.

College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China; Institute of Eco-Environmental Research, Guangxi Academy of Sciences, Nanning, 530007, China; Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai 528478, China. Electronic address:

Organic pollutants such as di-(2-ethylhexyl) phthalate (DEHP) interact with nanoplastics (NPs) and change their bioavailability and toxicity to aquatic organisms. This study aims to assess the ecotoxicological impacts of NPs in the presence and absence of DEHP on juvenile largemouth bass (LMB) Micropterus salmoides. Therefore, LMB was fed with diets containing various concentrations (0, 2, 10, and 40 mg/g) of polystyrene nanoplastics (PSNPs) by the weight of diets. After a 21-day of PSNPs dietary exposure, LMB was treated with DEHP at 450 μg/L through waterborne exposure for three days. Our results showed that PSNPs were accumulated in the intestinal tissues, which significantly decreased the feeding and growth rates in LMB. The histopathological analysis showed the intestine and liver of LMB were subjected to various degrees of structural damage caused by PSNPs, and DEHP-PSNP co-exposure enhanced those histopathological damages in both tissues. Additionally, the co-exposure induced oxidative stress in terms of increased activities of glutathione S-transferase, catalase, and superoxide dismutase enzymes in the liver, intestine, spleen, and serum. Furthermore, the co-exposure significantly changed the intestinal microbial composition, i.e., the decrease in the abundance of probiotics (Bacteroidetes and Proteobacteria) and the increase in pathogenic bacteria (Firmicutes) posed a great threat to fish metabolism and health. Therefore, this study highlights that the presence of DEHP enhances the toxicity of NPs on LMB in freshwater and suggests the regulated use of plastic and its additives for improving the health status of aquaculture fish for food safety in humans.
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http://dx.doi.org/10.1016/j.watres.2022.118608DOI Listing
May 2022

[Combination therapy based on PD-1/PD-L1 blocking in the treatment of non-small cell lung cancer].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2022 May;38(5):472-478

Department of Medical Microbiology and Parasitology, School of Basic Medicine, Air Force Medical University, Xi'an 710032, China. *Corresponding author, E-mail:

Non-small cell lung cancer (NSCLC) is one of the most serious malignancies and a serious threat to patients' quality of life. Traditional treatment have limitations such as side-effects, recurrence, drug resistance and lack of specificity. A novel therapy with immune checkpoint blocking has marked a breakthrough in the field of tumor therapy. It transforms the target of tumor therapy from a single molecule to immune cells, and provides a new strategy with a focus on the regulation of T cell function to control tumor progression. Programmed death 1 (PD-1) is the most well-studied immune checkpoint molecules. In recent years, profound progress was made in developing immunotherapies targeting the PD-1/PD-L1 signaling pathway, particularly in the treatment of NSCLC, given their high specificity and low side-effects. Nevertheless, there are still a considerable number of patients who have no significant therapeutic effect after receiving single antibodies that block the PD-1/PD-L1 pathway. Studies have shown that immunotherapy targeting the PD-1/PD-L1 pathway in the combination with other therapies can significantly improve survival rate and quality of life in patients. Such as radiotherapy and chemotherapy, dual immune checkpoint intervention, immunomodulator intervention, and even intestinal flora regulation can also be combined with anti-PD-1 /PD-L1 antibody for clinical treatment of NSCLC. These combination therapies have their own strengths and limitations, and their synergy mechanism requires further study. For NSCLC, more potential combined therapies based on PD-1/PD-L1 blockade are also being explored, laying a foundation for improving the survival rate and quality of life of NSCLC patients.
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May 2022

Paternal Radiofrequency Electromagnetic Radiation Exposure Causes Sex-Specific Differences in Body Weight Trajectory and Glucose Metabolism in Offspring Mice.

Front Public Health 2022 6;10:872198. Epub 2022 May 6.

Department of Gynecology and Obstetrics, Tangdu Hospital, Air Force Medical University, Xi'an, China.

Nowadays, concerns about the harmful effects of radiofrequency electromagnetic radiation (RF-EMR) on male fertility and offspring health are growing. In the present study, we investigated the effects of long-term exposure (at least 10 weeks) to the RF-EMR [2.0 GHz; power density, 2.5 W/m; whole-body specific absorption rate (SAR), 0.125-0.5 W/kg] on male mice fertility and F1 growth and glucose metabolism. No significant injuries were observed in testis organization, sperm quality, and pregnancy rate. However, mice exposed to RF-EMR exhibited a significantly elevated apoptosis rate in testis germ cells. Interestingly, paternal RF-EMR exposure resulted in sex-specific weight trajectory differences and glucose metabolism changes in male F1 mice but not in female F1 mice. The changed glucose metabolism in F1 male may result from the altered gene expression of liver Gck. These data collectively suggested that 2.0 GHz RF-EMR whole-body exposure of male mice does not cause obvious impairment in testis, sperm quality, and pregnancy rate. Paternal RF-EMR exposure causes male-specific alterations in body weight trajectories and glucose metabolism of F1.
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http://dx.doi.org/10.3389/fpubh.2022.872198DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120541PMC
May 2022

College Students with Oral Habits Exhibit Worse Psychological Status and Temporomandibular-Related Quality of Life: A Correlational Study.

Pain Res Manag 2022 11;2022:6079241. Epub 2022 May 11.

National Clinical Research Center for Oral Diseases, State Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, No. 14, Section 3, Ren Min South Road, Chengdu 610041, China.

Purpose: To evaluate the relationship between oral habits, psychological status, and temporomandibular-related quality of life among college students.

Materials And Methods: An online questionnaire was sent to college students who were willing to participate in this anonymous survey, which contained questions about the demographic characteristics of the participants, the Patient Health Questionnaire for Depression and Anxiety (PHQ-4), the Fonseca Anamnestic Index (FAI), and the Oral Health Impact Profile for temporomandibular disorders (OHIP-TMDs).

Results: A total of 505 valid questionnaires were collected from 200 males and 305 females (a mean age of 21.81 ± 2.81 years). The prevalence of oral habits in college students was 58% (294/505). Female gender (odds ratio (OR) 1.786) and having oral habits (OR 1.893) were associated with depression and anxiety. Medical students had significantly less depression and anxiety (OR 0.459) than nonmedical students. The possibility of suffering from temporomandibular disorder (TMDs) as evidenced by the OHIP-TMDs score was associated with female gender (OR 1.989) and having oral habits (OR 3.482). Students with oral habits had higher OHIP-TMDs scores.

Conclusion: More than half of the college students surveyed had specific oral habits, with a higher prevalence in women than in men. Having oral habits was related to a worse psychological status, higher risk of TMD, and worse temporomandibular-related quality of life.
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http://dx.doi.org/10.1155/2022/6079241DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117048PMC
May 2022

CoO crystal plane regulation to efficiently activate peroxymonosulfate in water: The role of oxygen vacancies.

J Colloid Interface Sci 2022 May 13;623:520-531. Epub 2022 May 13.

Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Materials Science and Chemical Engineering, Harbin Engineering University, Harbin 150001, China. Electronic address:

Crystal plane effect has attracted remarkable attention in the process of peroxymonosulfate (PMS) activation in water. In this work, nanocube-CoO (CoO-NC), nanoplate-CoO (CoO-NP) and nanorod-like CoO (CoO-NR) with (100), (111) and (110) plane predominant exposure is prepared by a facile hydrothermal method. CoO-NR with (110) plane exposed possesses more lattice defects (oxygen vacancies, Ov) and low oxidation state Co (Co), consequently, it exhibits a superior activity for PMS activation to efficiently remove bisphenol A (BPA) in water. Furthermore, it could be used in a widely water pH values ranging from 5.0 to 9.0 with an excellent PMS activited effects. During CoO-NR/PMS oxidation process, it is found that singlet oxygen (O) plays a dominant role in BPA degradation. However, CoO-NR treated by HO shows a poor PMS activation performance, confirming Ov acting as the active site during such oxidation process. The important effect of dissolved oxygen is tested by Ar introduction into the reaction system and the Ov-O* metastable intermediate is proposed. In situ Raman proves the interaction between dissolved oxygen and Ov and then the intermediate activates PMS to degrade BPA. This work not only explores the effect of different crystal plane exposures on PMS activation in CoO/PMS system, but investigates the evolution of Ov during the PMS activation.
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http://dx.doi.org/10.1016/j.jcis.2022.05.045DOI Listing
May 2022

Construction and validation of a novel apoptosis-associated prognostic signature related to osteosarcoma metastasis and immune infiltration.

Transl Oncol 2022 May 19;22:101452. Epub 2022 May 19.

Department of Orthopedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopedics, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, PR China. Electronic address:

Background: Apoptosis played vital roles in the formation and progression of osteosarcoma. However, no studies elucidated the prognostic relationships between apoptosis-associated genes (AAGs) and osteosarcoma.

Methods: The differentially expressed genes associated with osteosarcoma metastasis and apoptosis were identified from GEO and MSigDB databases. The apoptosis-associated prognostic signature was established through univariate and multivariate cox regression analyses. The Kaplan-Meier (KM) survival curve, ROC curve and nomogram were constructed to investigate the predictive value of this signature. CIBERSORT algorithm and ssGSEA were used to explore the relationships between immune infiltration and AAG signature. The above results were validated in another GEO dataset and the expression of AAGs was also validated in osteosarcoma patient samples by immunohistochemistry.

Results: HSPB1 and IER3 were involved in AAG signature. In training and validation datasets, apoptosis-associated risk scores were negatively related to patient survival rates and the AAG signature was regarded as the independent prognostic factor. ROC and calibration curves demonstrated the signature and nomogram were reliable. GSEA revealed the signature related to immune-associated pathways. ssGSEA indicated that one immune cell and three immune functions were significantly dysregulated. The immunohistochemistry analyses of patients' samples revealed that AAGs were significantly differently expressed between metastasis and non-metastasis osteosarcomas.

Conclusions: The present study identified and validated a novel apoptosis-associated prognostic signature related to osteosarcoma metastasis. It could serve as the potential biomarker and therapeutic targets for osteosarcoma in the future.
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http://dx.doi.org/10.1016/j.tranon.2022.101452DOI Listing
May 2022

KMT2B promotes the growth of renal cell carcinoma via upregulation of SNHG12 expression and promotion of CEP55 transcription.

Cancer Cell Int 2022 May 21;22(1):197. Epub 2022 May 21.

NHC Key Laboratory of Nuclear Technology Medical Transformation, Mianyang Central Hospital, School of Medicine, University of Electronic Science and Technology of China, No. 12 Changjia Lane, Jingzhong Street, Mianyang, 621000, People's Republic of China.

Background: This study aims to clarify the mechanistic action of long non-coding RNA (lncRNA) SNHG12 in the development of renal cell carcinoma (RCC), which may be associated with promoter methylation modification by KMT2B and the regulation of the E2F1/CEP55 axis.

Methods: TCGA and GEO databases were used to predict the involvement of SNHG12 in RCC. Knockdown of SNHG12/E2F1/CEP55 was performed. Next, SNHG12 expression and other mRNAs were quantified by RT-qPCR. Subsequently, CCK-8 was used to detect cell proliferation. Wound healing assay and Transwell assay were used to detect cell migration and invasion, respectively. The in vitro angiogenesis of human umbilical vein endothelial cells (HUVECs) was explored by matrigel-based capillary-like tube formation assay. ChIP assay was used to detect H3K4me3 in SNHG12 promoter region. The binding of E2F1 to CEP55 promoter region was analyzed with ChIP and dual luciferase reporter assays. RIP assay was used to detect the binding of SNHG12 to E2F1. Finally, the effect of SNHG12 on the tumor formation and angiogenesis of RCC was assessed in nude mouse xenograft model.

Results: SNHG12 was highly expressed in RCC tissues and cells, and it was related to the poor prognosis of RCC patients. SNHG12 knockdown significantly inhibited RCC cell proliferation, migration, and invasion and HUVEC angiogenesis. KMT2B up-regulated SNHG12 expression through modifying H3K4me3 in its promoter region. In addition, SNHG12 promoted CEP55 expression by recruiting the transcription factor E2F1. Knockdown of SNHG12 blocked E2F1 recruitment and down-regulated the expression of CEP55, thereby inhibiting tumor formation and angiogenesis in nude mice.

Conclusion: The evidence provided by our study highlighted the involvement of KMT2B in up-regulation of lncRNA as well as the transcription of CEP55, resulting in the promotion of angiogenesis and growth of RCC.
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http://dx.doi.org/10.1186/s12935-022-02607-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123657PMC
May 2022

Toxic effects of polystyrene nanoplastics and polybrominated diphenyl ethers to zebrafish (Danio rerio).

Fish Shellfish Immunol 2022 May 19;126:21-33. Epub 2022 May 19.

College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China; Institute of Eco-Environmental Research, Guangxi Academy of Sciences, Nanning, 530007, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangzhou, 510006, China. Electronic address:

Nanoplastics (NPs) are good carriers of persistent organic pollutants (POPs) such as polybrominated diphenyl ethers (PBDEs), and can alter their bioavailability and toxic impacts to aquatic organisms. This study highlights the single and combined toxic effects of polystyrene nanoplastics (PS-NPs) and 2,2',4,4'-tetrabromodiphenyl ether (BDE-47, one of the dominant congeners of PBDEs) on zebrafish embryos after an exposure duration of up to 120 hpf. Results showed that PS-NPs and BDE-47 co-exposure exacerbated the morphological deformities in terms of pericardial edema, yolk sac edema and curved tail in zebrafish larvae. Compared to BDE-47 single exposure, the combined exposure caused lower survival rates, shorter body lengths, and accelerated spontaneous movements. Further, PS-NPs were quickly aggregated on the surface of the embryonic chorions covered almost the entire membrane at 12 and 48 hpf, and concentration dependent accumulation was also found in the brain, mouth, trunk, gills, heart, liver and gastrointestinal tract at the larval stages. During the recovery period (7 days), PS-NPs were released from all the organs, with the highest elimination from the gastrointestinal tract. Histopathological examination revealed that co-exposure caused greater damage to retinal structures, muscle fibers and cartilage tissues. Responses of hypothalamic-pituitary-thyroid axis (CRH, TSHβ, NIS, TTR, Dio2, TG, TRα and TRβ) and reproduction (Esr2 and Vtg1) related genes were also investigated, and results showed that the co-exposure induced more significant upregulated expressions of TSHβ, TG, Doi 2, and TRβ, compared to BDE-47 single exposure. In conclusion, co-exposure to NPs and BDE-47 exacerbated developmental and thyroid toxicity in zebrafish, generally elucidating the toxicological effects mediated by complex chemical interactions between NPs with POPs in the freshwater environment.
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http://dx.doi.org/10.1016/j.fsi.2022.05.025DOI Listing
May 2022

Ghrelin-Ghrelin receptor (GSHR) pathway via endocannabinoid signal affects the expression of NPY to promote the food intake of Siberian sturgeon (Acipenser baerii).

Horm Behav 2022 May 18;143:105199. Epub 2022 May 18.

Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211#, Huimin Road, Chengdu, China. Electronic address:

Previous data suggested that activation of endocannabinoid receptor 1 (CB1) was necessary for the orexigenic effect of Ghrelin in rodents, but the information is limited in teleosts. To investigate the feeding regulation pathway of Ghrelin and CB1 in Siberian sturgeon (Acipenser baerii), this study first identified the Ghrelin (345 bp, complete coding sequence) and Ghrelin receptor (GHSR, 500 bp, partial coding sequence) sequences, and then detected their tissue distribution patterns, which showed that Ghrelin is mainly distribution in peripheral tissues, while GSHR is mainly in different brain divisions. Besides, the qPCR before and after feeding showed that the mRNA expressions of Ghrelin and GHSR were inhibited after feeding in telencephalon, diencephalon and mesencephalon. Subsequently, the food intake and appetite factor expressions were measured by i.c.v. co-injection of Ghrelin and GSHR antagonist. The results showed that Ghrelin promoted the food intake of Siberian sturgeon, which was reversed by its receptor antagonist. Besides, i.c.v. injection of Ghrelin decreased telencephalon CART expression while increased NPY expression in the three brain regions. In addition, to further explore the relationship of Ghrelin and CB1 signal regulating feeding, the co-injection of Ghrelin and CB1 antagonists was performed. The results showed that AM6545 (CB1 peripheral restricted antagonist) failed to affect the orexigenic effect of Ghrelin and the expression pattern of NPY mRNA in the telencephalon. While in the diencephalon, the increase of food intake and NPY mRNA expression induced by Ghrelin was completely reversed by Rimonabant (CB1 global antagonist). These results indicate Ghrelin-GSHR pathway promotes the food intake of Siberian sturgeon by inducing the expression of NPY in the diencephalon, and the stimulating effect will be reversed by cannabinoid receptor antagonism. This study provides a foundation for understanding the pathways Ghrelin and CB1 signals in appetite regulation of the teleost.
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http://dx.doi.org/10.1016/j.yhbeh.2022.105199DOI Listing
May 2022

A review on the remediation of microplastics using constructed wetlands: Bibliometric, co-occurrence, current trends, and future directions.

Chemosphere 2022 May 17;303(Pt 1):134990. Epub 2022 May 17.

College of Resources and Environment, Key Laboratory of Agricultural Environment, Shandong Agricultural University, Tai'an, Shandong, 271000, PR China.

Massive prevalence of microplastics (MPs) in the environment has become one of the world's most serious environmental concerns. Human dependence on plastics has created a constant flow of MPs from different sources into natural environment, which has raised public concern regarding consequences of MPs coming into contact with the natural environment. Deploying constructed wetlands (CWs) to reduce MPs pollution is considered a promising method, however there are still barriers for breakthroughs in this technology, particularly knowledge gaps in the mechanisms affect removal process. Recognising this, we provide a comprehensive summary of current advances and theories regarding the mechanisms of occurrence in this research area. In this work, the bibliometric methods were first used to identify annual publication trends and topical topics of research interest. The selected documents were then statistically analyzed using VOSviewer and the 'bibliometrix' package in R to derive the annual productivity of countries or organizations, the most relevant affiliations, the most relevant authors, the most relevant sources, textual analysis, co-occurrence analysis, and cluster analysis of keywords. Finally, detailed information concerning the removal of MPs by CWs was summarised, covering the most common operational and design parameters (i.e., structure types, wetland plants, substrate materials, and microbial communities), to reveal how these parameters can be adjusted for more efficient MPs removal rate. Challenges and future directions were additionally proposed. It is hoped that the review will help identify current research trends, provide insight into the mechanisms of the removal process, and contribute further to the development of this important area.
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http://dx.doi.org/10.1016/j.chemosphere.2022.134990DOI Listing
May 2022

Transgenerational effects of parental bisphenol S exposure on zebrafish (Danio rerio) reproduction.

Food Chem Toxicol 2022 May 18;165:113142. Epub 2022 May 18.

College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China. Electronic address:

Bisphenol S (BPS) is extensively used for production of polycarbonates and other commodities, and is often detected in environment and biota. Parental BPS exposure has been reported to interfere with reproductive development of offspring, but limited information is available on its multigenerational reproductive toxicity. In our present study, zebrafish (Danio rerio) were exposed to BPS (1 and 100 μg/L) from 3 hpf to 120 dpf, and the effects on reproduction, sex steroid hormones, DNA methylation levels and gene transcription involved in steroidogenesis and DNA methylation were investigated in unexposed F1-2 offspring. The results showed that 100 μg/L BPS exposure increased DNA methylation in F1 testes, and 1 μg/L BPS led to DNA methylation in F2 ovaries. The increased DNA methylation levels led to decreased expression of steroidogenic enzymes, including cyp11a, cyp17 and 3βhsd, which might be a main reason for the elevated plasma 17β-estradiol and decreased testosterone levels. In addition, sex ratio indicated a female dominance trend, and reproductive capacity of male fish was severely impaired. Overall, these findings suggest that parental BPS exposure impairs reproductive development of unexposed offspring via DNA methylation and BPS-induced epigenetic modification inheritance has a long-term effect on the fitness and sustainability of fish populations.
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http://dx.doi.org/10.1016/j.fct.2022.113142DOI Listing
May 2022

Multivariate time series forecasting method based on nonlinear spiking neural P systems and non-subsampled shearlet transform.

Neural Netw 2022 May 7;152:300-310. Epub 2022 May 7.

School of Computer and Software Engineering, Xihua University, Chengdu, 610039, China.

Multivariate time series forecasting remains a challenging task because of its nonlinear, non-stationary, high-dimensional, and spatial-temporal characteristics, along with the dependence between variables. To address this limitation, we propose a novel method for multivariate time series forecasting based on nonlinear spiking neural P (NSNP) systems and non-subsampled shearlet transform (NSST). A multivariate time series is first converted into the NSST domain, and then NSNP systems are automatically constructed, trained, and predicted in the NSST domain. Because NSNP systems are used as nonlinear prediction models and work in the NSST domain, the proposed prediction method is essentially a multiscale transform (MST)-based prediction method. Therefore, the proposed prediction method can process nonlinear and non-stationary time series, and the dependence between variables can be characterized by the multiresolution features of the NSST transform. Five real-life multivariate time series were used to compare the proposed prediction method with five state-of-the-art and 28 baseline prediction methods. The comparison results demonstrate the effectiveness of the proposed method for multivariate time-series forecasting.
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http://dx.doi.org/10.1016/j.neunet.2022.04.030DOI Listing
May 2022

Nanopore Sequencing of Full rRNA Operon Improves Resolution in Mycobiome Analysis and Reveals High Diversity in Both Human Gut and Environments.

Mol Ecol 2022 May 20. Epub 2022 May 20.

CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

High-throughput sequencing has substantially improved our understanding of fungal diversity. However, the short read (<500 bp) length of current second-generation sequencing approaches provides limited taxonomic and phylogenetic resolution for species discrimination. Longer sequences containing more information are highly desired to provide greater taxonomic resolution. Here, we amplified full-length rRNA operons (~ 5.5 kb) and established a corresponding Fungal rRNA Operon Database for ONT sequences (FRODO), which contains ONT sequences representing 8 phyla, 41 classes, 109 orders, 256 families, 524 genera and 1,116 species. We also benchmarked the optimal method for sequence classification and determined that the RDP classifier based on our FRODO database was capable of improving the classification of ONT reads, with an average of 98%-99% reads correctly classified at the genus or species level. We investigated the applicability of our approach in three representative mycobiomes, namely, the soil, marine and human gut mycobiomes, and found that the gut contains the largest number of unknown species (over 90%), followed by the marine (42%) and soil (33.8%) mycobiomes. We also observed a distinct difference in the composition of the marine and soil mycobiomes, with the highest richness and diversity detected in soils. Overall, our study provides a systematic approach for mycobiome studies and revealed that the previous methods might have underestimated the diversity of mycobiome species. Future application of this method will lead to a better understanding of the taxonomic and functional diversity of fungi in environmental and health-related mycobiomes.
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http://dx.doi.org/10.1111/mec.16534DOI Listing
May 2022

Spatial metabolomics for evaluating response to neoadjuvant therapy in non-small cell lung cancer patients.

Cancer Commun (Lond) 2022 May 20. Epub 2022 May 20.

Research Unit Analytical Pathology, Helmholtz Zentrum München - German Research Center for Environmental Health, Neuherberg, 85764, Germany.

Background: The response to neoadjuvant chemotherapy (NAC) differs substantially among individual patients with non-small cell lung cancer (NSCLC). Major pathological response (MPR) is a histomorphological read-out used to assess treatment response and prognosis in patients NSCLC after NAC. Although spatial metabolomics is a promising tool for evaluating metabolic phenotypes, it has not yet been utilized to assess therapy responses in patients with NSCLC. We evaluated the potential application of spatial metabolomics in cancer tissues to assess the response to NAC, using a metabolic classifier that utilizes mass spectrometry imaging combined with machine learning.

Methods: Resected NSCLC tissue specimens obtained after NAC (n = 88) were subjected to high-resolution mass spectrometry, and these data were used to develop an approach for assessing the response to NAC in patients with NSCLC. The specificities of the generated tumor cell and stroma classifiers were validated by applying this approach to a cohort of biologically matched chemotherapy-naïve patients with NSCLC (n = 85).

Results: The developed tumor cell metabolic classifier stratified patients into different prognostic groups with 81.6% accuracy, whereas the stroma metabolic classifier displayed 78.4% accuracy. By contrast, the accuracies of MPR and TNM staging for stratification were 62.5% and 54.1%, respectively. The combination of metabolic and MPR classifiers showed slightly lower accuracy than either individual metabolic classifier. In multivariate analysis, metabolic classifiers were the only independent prognostic factors identified (tumor: P = 0.001, hazards ratio [HR] = 3.823, 95% confidence interval [CI] = 1.716-8.514; stroma: P = 0.049, HR = 2.180, 95% CI = 1.004-4.737), whereas MPR (P = 0.804; HR = 0.913; 95% CI = 0.445-1.874) and TNM staging (P = 0.078; HR = 1.223; 95% CI = 0.977-1.550) were not independent prognostic factors. Using Kaplan-Meier survival analyses, both tumor and stroma metabolic classifiers were able to further stratify patients as NAC responders (P < 0.001) and non-responders (P < 0.001).

Conclusions: Our findings indicate that the metabolic constitutions of both tumor cells and the stroma are valuable additions to the classical histomorphology-based assessment of tumor response.
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http://dx.doi.org/10.1002/cac2.12310DOI Listing
May 2022

Myeloid cell-specific topoisomerase 1 inhibition using DNA origami mitigates neuroinflammation.

EMBO Rep 2022 May 20:e54499. Epub 2022 May 20.

Applied Immunology and Immunotherapy, Department of Clinical Neuroscience, Karolinska Institutet, Center for Molecular Medicine, Karolinska University Hospital, Stockholm, Sweden.

Targeting myeloid cells, especially microglia, for the treatment of neuroinflammatory diseases such as multiple sclerosis (MS), is underappreciated. Our in silico drug screening reveals topoisomerase 1 (TOP1) inhibitors as promising drug candidates for microglial modulation. We show that TOP1 is highly expressed in neuroinflammatory conditions, and TOP1 inhibition using camptothecin (CPT) and its FDA-approved analog topotecan (TPT) reduces inflammatory responses in microglia/macrophages and ameliorates neuroinflammation in vivo. Transcriptomic analyses of sorted microglia from LPS-challenged mice reveal an altered transcriptional phenotype following TPT treatment. To target myeloid cells, we design a nanosystem using β-glucan-coated DNA origami (MyloGami) loaded with TPT (TopoGami). MyloGami shows enhanced specificity to myeloid cells while preventing the degradation of the DNA origami scaffold. Myeloid-specific TOP1 inhibition using TopoGami significantly suppresses the inflammatory response in microglia and mitigates MS-like disease progression. Our findings suggest that TOP1 inhibition in myeloid cells represents a therapeutic strategy for neuroinflammatory diseases and that the myeloid-specific nanosystems we designed may also benefit the treatment of other diseases with dysfunctional myeloid cells.
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http://dx.doi.org/10.15252/embr.202154499DOI Listing
May 2022

The evaluation of portal hypertension in cirrhotic patients with spectral computed tomography.

Acta Radiol 2022 May 20:2841851221101356. Epub 2022 May 20.

Department of Interventional Oncology, Renji Hospital, 71140Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.

Background: Iodine concentrations measured using dual-energy spectral CT (DESCT) have been recently proposed as providing good performance for examining tissues hemodynamics.

Purpose: To evaluate the diagnostic efficacy of DESCT-derived parameters in evaluating portal venous pressure in patients with liver cirrhosis.

Material And Methods: A total of 71 patients with liver cirrhosis who underwent percutaneous transhepatic portal vein puncture procedures were included in this study. All participants underwent DESCT and gastrointestinal endoscopy within one month before the operation. The direct portal venous pressure of each participant was measured preoperatively.

Results: Stepwise multivariate linear regression analysis showed that the iodine concentrations in the portal vein and hepatic parenchyma during the portal venous phase and the platelet count were independently correlated with the direct portal venous pressure ( < 0.001,  < 0.001, and  = 0.030, respectively). Receiver operating characteristic analysis revealed that the normalized iodine concentration of the hepatic parenchyma had the best performance for identifying clinically significant portal hypertension (≥10 mmHg), esophageal varices, and high-risk esophageal varices (the area under the curve values were 0.951, 0.932, and 0.960, respectively).

Conclusion: The normalized iodine concentration of the hepatic parenchyma is a reliable parameter to non-invasively assess portal venous pressure in patients with liver cirrhosis.
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http://dx.doi.org/10.1177/02841851221101356DOI Listing
May 2022

Seismic Performance of Steel-Reinforced Concrete Columns with Q690 High-Strength Steel.

Materials (Basel) 2022 Apr 19;15(9). Epub 2022 Apr 19.

School of Civil Engineering, Northeast Forestry University, Harbin 150000, China.

In this paper, based on the low-cycle loading tests of 11 steel-reinforced concrete (SRC) frame columns with built-in Q690 steel and 5 SRC frame columns with built-in Q235 steel, a systematic study on their seismic performance was carried out. The design parameters of the specimens were the steel strength, axial compression ratio, shear span ratio, steel content, and stirrup ratio. The failure modes, stress characteristics, hysteresis curve, skeleton curve, displacement ductility performance, energy dissipation capacity, and other main seismic indicators of the specimens with different parameters were analyzed, and the corresponding relationship between the displacement ductility performance of the specimen and the energy dissipation capacity and design parameters was obtained. The results show that the load-displacement curve of the specimens is relatively full, the descending section is gentle, and various seismic performance indicators are relatively excellent, reflecting good seismic performance. Equipped with high-strength steel SRC frame columns, they can better bear the horizontal load, the displacement ductility performance is improved, and the energy dissipation capacity is slightly lower than that of ordinary-strength steel SRC frame columns. The increase in the shear span ratio, steel content, and stirrup ratio of the specimens helps to improve their seismic performance, whereas an increase in the axial compression ratio makes their seismic performance worse.
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http://dx.doi.org/10.3390/ma15092979DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104870PMC
April 2022

Performance of PROPELLER FSE TWI in reducing metal artifacts of material porcelain fused to metal crown: a clinical preliminary study.

Sci Rep 2022 May 19;12(1):8442. Epub 2022 May 19.

Department of Radiology, The Second Hospital of Shanxi Medical University, NO.382 Wuyi Road, Taiyuan, 030001, Shanxi, China.

This study aimed to compare MRI quality between conventional fast spin echo T weighted imaging (FSE TWI) with periodically rotated overlapping parallel lines with enhanced reconstruction (PROPELLER) FSE TWI for patients with various porcelain fused to metal (PFM) crown and analyze the value of PROPELLER technique in reducing metal artifacts. Conventional FSE TWI and PROPELLER FSE TWI sequences for axial imaging of head were applied in participants with different PFM crowns: cobalt-chromium (Co-Cr) alloy, pure titanium (Ti), gold-palladium (Au-Pd) alloy. Two radiologists evaluated overall image quality of section in PFM using a 5-point scale qualitatively and measured the maximum artifact area and artifact signal-to-noise ratio (SNR) quantitatively. Fifty-nine participants were evaluated. The metal crown with the least artifacts and the optimum image quality shown in conventional FSE TWI and PROPELLER FSE TWI were in Au-Pd alloy, Ti, and Co-Cr alloy order. PROPELLER FSE TWI was superior to conventional FSE TWI in improving image quality and reducing artifact area for Co-Cr alloy (17.0 ± 0.2% smaller artifact area, p < 0.001) and Ti (11.6 ± 0.7% smaller artifact area, p = 0.005), but had similar performance compared to FSE TWI for Au-Pd alloy. The SNRs of the tongue and masseter muscle were significantly higher on PROPELLER FSE TWI compared with conventional FSE TWI (tongue: 29.76 ± 8.45 vs. 21.54 ± 9.31, p = 0.007; masseter muscle: 19.11 ± 8.24 vs. 15.26 ± 6.08, p = 0.016). Therefore, the different PFM crown generate varying degrees of metal artifacts in MRI, and the PROPELLER can effectively reduce metal artifacts especially in the PFM crown of Co-Cr alloy.
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http://dx.doi.org/10.1038/s41598-022-12402-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120134PMC
May 2022

An improved calibrated mass spectrometry for absolute copper isotope-ratio measurement using two strategies for mass bias correction factor.

Anal Chim Acta 2022 Jun 3;1211:339905. Epub 2022 May 3.

Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing, 100029, PR China.

Modern advances in multi-collector inductively coupled plasma mass spectrometry (MC-ICPMS) have greatly promoted the investigation of copper isotopes in various research fields. To meet the demand, an improved calibrated mass spectrometry for the absolute determination of copper isotopic composition was developed in this study. The method has made progress in the investigation of instrumental mass bias correction factor for copper isotopic analysis using MC-ICPMS through employing two independent strategies. One is the conventional mathematical iterative method based on the MC-ICPMS measurement data; the other one is direct calculation using the absolute copper isotopic compositions in isotopically enriched Cu and Cu materials by developing a TE-TIMS method. The results of K = 0.95563(16) and K = 0.95551(17) were consistent in their standard uncertainties, demonstrated the first cross-validation of these two strategies. Thereafter, the proposed calibrated mass spectrometry were applied to re-measure the copper isotope amount ratios in three isotopic reference materials, resulted in R = 0.44547(16), R = 0.44549(16), and R = 0.44557(16), respectively. These expended uncertainties were only one third or one fifth of the stated uncertainties in their certificates. Furthermore, the absolute copper isotopic compositions in four natural-abundance samples and a new copper isotopic reference material GBW04624 were determined, given an average atomic weight of copper as A(Cu) = 63.5456(4). The δCu values of GBW04624 relative to NIST SRM 976, ERM®-AE633 and ERM®-AE647 were also applied by implementing the C-SSBIN calibration model. This provides an additional independently certified and SI-traceable copper isotopic reference material, in support of various scientific researches.
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http://dx.doi.org/10.1016/j.aca.2022.339905DOI Listing
June 2022

A new species from Xinjiang Uygur Autonomous Region (China) with a key to Xinjiang freshwater gammarids (Crustacea, Amphipoda, Gammaridae).

Zookeys 2022 25;1090:129-147. Epub 2022 Mar 25.

Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affair/ Engineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China Huazhong Agricultural University Wuhan China.

A new species of the genus Fabricius, 1775 is described and illustrated from Xinjiang Uygur Autonomous Region, China. is characterized by pereopods III-IV with long straight setae on posterior margins; inner ramus of uropod III more than twice as long as peduncle, reaching 0.7 times the length of outer ramus; inner ramus with plumose setae, and outer ramus with both plumose setae and long simple setae. Detailed morphological comparisons with related species are discussed. The K2P distances for each marker (CO1, 16S, 28S, and EF1α) of the new species differ from those of other species in Xinjiang. Both phylogenetic trees based on separate (CO1, 16S, 28S, and EF1α) and combined (CO1+16S+28S+EF1α) markers show that the new species is an independent branch. A key to identify species in Xinjiang is provided.
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http://dx.doi.org/10.3897/zookeys.1090.78834DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8975802PMC
March 2022

Exploring the spatiotemporal changes in carbon storage under different development scenarios in Jiangsu Province, China.

PeerJ 2022 13;10:e13411. Epub 2022 May 13.

Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.

Carbon storage (CS) is closely linked to the global challenge of climate change. Land use/cover (LULC) change is the main factor driving changes in CS, and evaluating the impact of LULC changes on CS is important for carbon balance. Taking Jiangsu Province as an example, we used the Integrated Valuation of Ecosystem Services and Trade-offs model to analyze the spatiotemporal changes in CS during 2000-2015. Then we coupled it with the patch-generating land use simulation model to simulate and predict LULC and CS in 2050 under four different development plans. The results showed that LULC change in Jiangsu Province was manifested mainly as transformation of cropland to construction land (3,485 km) and cropland to water body (470 km). The high value area for CS was concentrated mainly in forest land, water body and grassland, whereas the low value area was concentrated mainly in construction land. During 2000-2015, CS decreased by 0.23 Tg, and during 2015-2050, CS was predicted to decrease by 0.16, 1.69, 0.02, and 0.10 Tg under the baseline, fast, slow and harmonious development scenarios. The conversion of a large amount of cropland to construction land was the main cause of CS loss. In all scenarios, the carbon loss was the largest in southern Jiangsu and lowest in central Jiangsu. It is necessary to balance the conflict between economic development and ecological protection during the process of urbanization. This study can provide an important reference for decision makers during the formulation of regional development models and ecological management strategies.
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http://dx.doi.org/10.7717/peerj.13411DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9109690PMC
May 2022

The Wnt gene family in Tenebrio molitor and other coleopterans.

Arch Insect Biochem Physiol 2022 May 18:e21915. Epub 2022 May 18.

Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming, China.

The Wnt gene family is involved in a wide range of developmental processes. Despite its significance, the evolution and function of Wnt genes remain largely unclear. Here, an exhaustive survey of Wnt genes was conducted in Tenebrio molitor and 17 other beetle genomes. A total of 146 Wnt genes were identified, creating a comprehensive coleopteran Wnt gene catalog. Comparative genomics indicates that dynamic evolutionary patterns of Wnt gene loss and duplication occurred in Coleoptera, leading to the diverse Wnt gene repertoire in various beetles. A striking loss of particular Wnt gene subfamilies occurs in Coleoptera. Remarkably, Wnt gene duplication was discovered for the first time in insects. Further analysis of Wnt gene expression in T. molitor indicates that each Wnt gene, including the duplicated ones, has a unique spatial or temporal expression pattern. The current study provides valuable insight into the evolution and functional validation of Wnt genes in Coleoptera.
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http://dx.doi.org/10.1002/arch.21915DOI Listing
May 2022
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