John Aach - Harvard Medical School

John Aach

Harvard Medical School

United States

John Aach - Harvard Medical School

John Aach

Introduction

Primary Affiliation: Harvard Medical School - United States

Education

Sep 2017

Publications

35Publications

1647Reads

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4813PubMed Central Citations

Optimizing complex phenotypes through model-guided multiplex genome engineering.

Genome Biol 2017 05 25;18(1):100. Epub 2017 May 25.

Department of Genetics, Harvard Medical School, Boston, MA, USA.

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http://dx.doi.org/10.1186/s13059-017-1217-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5445303PMC
May 2017
65 Reads
4 Citations

Addressing the ethical issues raised by synthetic human entities with embryo-like features.

Elife 2017 03 21;6. Epub 2017 Mar 21.

Department of Genetics, Harvard Medical School, Boston, United States.

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http://dx.doi.org/10.7554/eLife.20674DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5360441PMC
March 2017
84 Reads
7 Citations
8.520 Impact Factor

Engineering and optimising deaminase fusions for genome editing.

Nat Commun 2016 11 2;7:13330. Epub 2016 Nov 2.

Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.

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http://dx.doi.org/10.1038/ncomms13330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5097136PMC
November 2016
74 Reads
11 Citations
10.742 Impact Factor

Genome-wide inactivation of porcine endogenous retroviruses (PERVs).

Science 2015 Nov 11;350(6264):1101-4. Epub 2015 Oct 11.

Department of Genetics, Harvard Medical School, Boston, MA, USA. Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA, USA. eGenesis Biosciences, Boston, MA 02115, USA.

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http://arep.med.harvard.edu/pdf/Yang_Science_2015.pdf
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http://science.sciencemag.org/content/early/2015/10/09/scien
Web Search
http://www.sciencemag.org/cgi/doi/10.1126/science.aad1191
Publisher Site
http://dx.doi.org/10.1126/science.aad1191DOI Listing
November 2015
220 Reads
75 Citations
31.480 Impact Factor

Improved cell-free RNA and protein synthesis system.

PLoS One 2014 2;9(9):e106232. Epub 2014 Sep 2.

Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0106232PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4152126PMC
May 2015
37 Reads
12 Citations
3.234 Impact Factor

Highly efficient Cas9-mediated transcriptional programming.

Nat Methods 2015 Apr 2;12(4):326-8. Epub 2015 Mar 2.

1] Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, Massachusetts, USA. [2] Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA.

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http://dx.doi.org/10.1038/nmeth.3312DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4393883PMC
April 2015
37 Reads
152 Citations
32.072 Impact Factor

Fluorescent in situ sequencing (FISSEQ) of RNA for gene expression profiling in intact cells and tissues.

Nat Protoc 2015 Mar 12;10(3):442-58. Epub 2015 Feb 12.

1] Wyss Institute, Harvard Medical School, Boston, Massachusetts, USA. [2] Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA.

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http://dx.doi.org/10.1038/nprot.2014.191DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4327781PMC
March 2015
41 Reads
56 Citations

Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells.

Nucleic Acids Res 2015 Feb 20;43(3):e21. Epub 2014 Nov 20.

Department of Genetics, Harvard Medical School, Boston, MA 02115, USA Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA

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http://dx.doi.org/10.1093/nar/gku1246DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4330342PMC
February 2015
6 Reads
37 Citations
9.112 Impact Factor

Multiplex single-molecule interaction profiling of DNA-barcoded proteins.

Nature 2014 Nov 21;515(7528):554-7. Epub 2014 Sep 21.

1] Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA [2] Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, USA.

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http://dx.doi.org/10.1038/nature13761DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4246050PMC
November 2014
8 Reads
13 Citations
42.351 Impact Factor

Targeted and genome-wide sequencing reveal single nucleotide variations impacting specificity of Cas9 in human stem cells.

Nat Commun 2014 Nov 26;5:5507. Epub 2014 Nov 26.

1] Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA [2] Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, USA.

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http://dx.doi.org/10.1038/ncomms6507DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4352754PMC
November 2014
53 Reads
45 Citations
10.742 Impact Factor

Rational optimization of tolC as a powerful dual selectable marker for genome engineering.

Nucleic Acids Res 2014 Apr 22;42(7):4779-90. Epub 2014 Jan 22.

Department of Genetics and Wyss Institute for Biologically Inspired Engineering, Harvard Medical School, Boston, MA 02115, USA, Program in Chemical Biology, Harvard University, Cambridge, MA 02138, USA, Biological and Biomedical Sciences, Harvard Medical School, Boston, MA 02115, USA and Molecular, Cellular, Developmental and Systems Biology Institute, Yale University, New Haven, CT 06516, USA.

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http://dx.doi.org/10.1093/nar/gkt1374DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985617PMC
April 2014
7 Reads
15 Citations
9.112 Impact Factor

Optimization of scarless human stem cell genome editing.

Nucleic Acids Res 2013 Oct 31;41(19):9049-61. Epub 2013 Jul 31.

Department of Genetics, Harvard Medical School, Boston, 02115 MA, USA, Biological and Biomedical Sciences Program, Harvard Medical School, Boston, 02115 MA, USA, Children's Hospital, Boston, 02115 MA, USA, Chemistry and Chemical Biology program, Harvard, 02138 Cambridge, MA, USA and Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, 02138 MA, USA.

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http://dx.doi.org/10.1093/nar/gkt555DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3799423PMC
October 2013
40 Reads
122 Citations
9.112 Impact Factor

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering.

Nat Biotechnol 2013 Sep 1;31(9):833-8. Epub 2013 Aug 1.

1] Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA. [2].

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http://dx.doi.org/10.1038/nbt.2675DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3818127PMC
September 2013
88 Reads
504 Citations
41.514 Impact Factor

Barcoding cells using cell-surface programmable DNA-binding domains.

Nat Methods 2013 May 17;10(5):403-6. Epub 2013 Mar 17.

Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA.

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http://dx.doi.org/10.1038/nmeth.2407DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3641172PMC
May 2013
9 Reads
13 Citations
32.072 Impact Factor

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems.

Nucleic Acids Res 2013 Apr 4;41(7):4336-43. Epub 2013 Mar 4.

Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA.

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http://dx.doi.org/10.1093/nar/gkt135DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3627607PMC
April 2013
86 Reads
323 Citations
9.112 Impact Factor

RNA-guided human genome engineering via Cas9.

Science 2013 Feb 3;339(6121):823-6. Epub 2013 Jan 3.

Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

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http://arep.med.harvard.edu/pdf/Mali_Sci_12.pdf
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http://mali.ucsd.edu/uploads/3/1/0/0/31002267/science_2013.p
Web Search
http://www.sciencemag.org/cgi/doi/10.1126/science.1232033
Publisher Site
http://dx.doi.org/10.1126/science.1232033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3712628PMC
February 2013
307 Reads
2139 Citations
31.480 Impact Factor

Proteome-wide systems analysis of a cellulosic biofuel-producing microbe.

Mol Syst Biol 2011 Jan;7:461

Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

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http://dx.doi.org/10.1038/msb.2010.116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3049413PMC
January 2011
3 Reads
30 Citations
10.872 Impact Factor

Personal genomes in progress: from the human genome project to the personal genome project.

Dialogues Clin Neurosci 2010 ;12(1):47-60

European Centre for Public Health Genomics, FHML, Maastricht University, Maastricht, The Netherlands.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3181947PMC
April 2010
4 Reads
24 Citations

Multiplex padlock targeted sequencing reveals human hypermutable CpG variations.

Genome Res 2009 Sep 12;19(9):1606-15. Epub 2009 Jun 12.

Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.

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http://dx.doi.org/10.1101/gr.092213.109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2752131PMC
September 2009
19 Reads
30 Citations
14.630 Impact Factor

Digital RNA allelotyping reveals tissue-specific and allele-specific gene expression in human.

Nat Methods 2009 Aug 20;6(8):613-8. Epub 2009 Jul 20.

Department of Bioengineering, University of California at San Diego, La Jolla, California, USA.

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http://dx.doi.org/10.1038/nmeth.1357DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2742772PMC
August 2009
31 Reads
82 Citations
32.072 Impact Factor

Genome-wide identification of human RNA editing sites by parallel DNA capturing and sequencing.

Science 2009 May;324(5931):1210-3

Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.

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http://dx.doi.org/10.1126/science.1170995DOI Listing
May 2009
2 Reads
216 Citations
31.480 Impact Factor

Identification of molecular markers of bipolar cells in the murine retina.

J Comp Neurol 2008 Apr;507(5):1795-810

Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.

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http://dx.doi.org/10.1002/cne.21639DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2665264PMC
April 2008
6 Reads
52 Citations
3.230 Impact Factor

Quantitative morphological signatures define local signaling networks regulating cell morphology.

Science 2007 Jun;316(5832):1753-6

Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

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http://dx.doi.org/10.1126/science.1140324DOI Listing
June 2007
15 Reads
100 Citations
31.480 Impact Factor

Global gene expression of Prochlorococcus ecotypes in response to changes in nitrogen availability.

Mol Syst Biol 2006 3;2:53. Epub 2006 Oct 3.

Department of Biology, MIT/WHOI Joint Program in Oceanography, Cambridge, MA, USA.

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http://dx.doi.org/10.1038/msb4100087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1682016PMC
November 2006
109 Reads
48 Citations
10.872 Impact Factor

Long-range polony haplotyping of individual human chromosome molecules.

Nat Genet 2006 Mar 19;38(3):382-7. Epub 2006 Feb 19.

Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.

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http://dx.doi.org/10.1038/ng1741DOI Listing
March 2006
2 Reads
20 Citations
29.352 Impact Factor

Accommodation or prediction?

Science 2005 Jun;308(5727):1409-12; author reply 1409-12

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June 2005
3 Reads
31.480 Impact Factor

Mathematical models of diffusion-constrained polymerase chain reactions: basis of high-throughput nucleic acid assays and simple self-organizing systems.

J Theor Biol 2004 May;228(1):31-46

Department of Genetics and Lipper Center for Computational Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, New Research Building, Rm 238, Boston, MA 02115, USA.

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http://dx.doi.org/10.1016/j.jtbi.2003.12.003DOI Listing
May 2004
3 Citations
2.120 Impact Factor

Computational and experimental identification of C. elegans microRNAs.

Mol Cell 2003 May;11(5):1253-63

The Lipper Center for Computational Genetics and Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

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http://dx.doi.org/10.1016/s1097-2765(03)00153-9DOI Listing
May 2003
5 Reads
102 Citations
14.020 Impact Factor

Automated modelling of signal transduction networks.

BMC Bioinformatics 2002 Nov 1;3:34. Epub 2002 Nov 1.

Dept of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts, 02115, USA.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC137599PMC
http://dx.doi.org/10.1186/1471-2105-3-34DOI Listing
November 2002
92 Reads
68 Citations
2.580 Impact Factor

Measuring absolute expression with microarrays with a calibrated reference sample and an extended signal intensity range.

Proc Natl Acad Sci U S A 2002 May;99(11):7554-9

Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.

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http://dx.doi.org/10.1073/pnas.112683499DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC124281PMC
May 2002
4 Reads
78 Citations
9.810 Impact Factor