Publications by authors named "Jie Chen"

5,802 Publications

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Simultaneous Extraction of Planetary Boundary-Layer Height and Aerosol Optical Properties from Coherent Doppler Wind Lidar.

Sensors (Basel) 2022 Apr 29;22(9). Epub 2022 Apr 29.

Key Laboratory of Atmospheric Optics, Anhui Institute of Optics and Fine Mechanics, HFIPS, Chinese Academy of Sciences, Hefei 230031, China.

Planetary boundary-layer height is an important physical quantity for weather forecasting models and atmosphere environment assessment. A method of simultaneously extracting the surface-layer height (SLH), mixed-layer height (MLH), and aerosol optical properties, which include aerosol extinction coefficient (AEC) and aerosol optical depth (AOD), based on the signal-to-noise ratio (SNR) of the same coherent Doppler wind lidar (CDWL) is proposed. The method employs wavelet covariance transform to locate the SLH and MLH using the local maximum positions and an automatic algorithm of dilation operation. AEC and AOD are determined by the fitting curve using the SNR equation. Furthermore, the method demonstrates the influential mechanism of optical properties on the SLH and MLH. MLH is linearly correlated with AEC and AOD because of solar heating increasing. The results were verified by the data of an ocean island site in China.
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http://dx.doi.org/10.3390/s22093412DOI Listing
April 2022

KAT2A affects tumor metabolic reprogramming in colon cancer progression through epigenetic activation of E2F1.

Hum Cell 2022 May 18. Epub 2022 May 18.

Department of Hernia and Abdominal Wall Surgery, Beijing Chao-Yang Hospital, Capital Medical University, No. 5 Jingyuan Road, Shijingshan District, Beijing, 100043, People's Republic of China.

Lysine acetyltransferase 2 A (KAT2A) has been implicated in tumorigenesis; nevertheless, the mechanism underlying its tumor-initiating effect remains elusive. In the present study, we aimed to identify the possible role of KAT2A in regulating metabolic reprogramming, a hallmark of cancer, in colon cancer (CC). KAT2A was found to be overexpressed in CC and correlated with metastases. KAT2A induced proliferation, migration, invasion, and epithelial-mesenchymal transition of CC cells, along with elevated cellular glycolytic capacity and mitochondrial stress. Functional enrichment analyses predicted and ChIP assays verified that KAT2A activated E2F transcription factor 1 (E2F1) by modifying the acetylation of H3K9. Rescue experiments revealed that E2F1 downregulation inhibited cellular activity, aerobic glycolysis and mitochondrial respiration in CC in the presence of KAT2A. Moreover, KAT2A/E2F1 promoted tumorigenic activity and lung metastases of CC cells in mice. Taken together, our findings demonstrate the substantial role of KAT2A in the modulation of post-translational modifications of E2F1 in CC, suggesting that knockdown of KAT2A may be a potential strategy for CC treatment.
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http://dx.doi.org/10.1007/s13577-022-00707-3DOI Listing
May 2022

HDL Mimetic Peptides.

Adv Exp Med Biol 2022 ;1377:141-151

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing, China.

Since therapeutic strategies designed to raise HDL failed to exert the expected effective cardiovascular disease (CVD) outcomes in clinical trials, how to improve HDL function rather than its plasma level has become a new focus of scientists' attention. Numerous HDL mimetic peptides have been designed and investigated in various animal models in recent years. Although the underlying mechanisms are not fully understood, the peptides' antiatherogenic effects, such as acceleration of RCT and improvement of natural HDL function without necessarily raising its level, showed a promising therapeutic role in the prevention of atherosclerosis and other diseases. This chapter reviews recent studies on the roles and potentials of HDL mimetic peptides in atherosclerosis-related CVD.
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http://dx.doi.org/10.1007/978-981-19-1592-5_11DOI Listing
May 2022

HDL and Endothelial Function.

Adv Exp Med Biol 2022 ;1377:27-47

Lipid Research Group, School of Medical Sciences, Level 4E, Wallace Wurth Building, University of New South Wales Sydney, Sydney, NSW, Australia.

Plasmid high-density lipoprotein (HDL) is a critical biomarker in predicting cardiovascular diseases. Endothelial cells are physically located in the intima of blood vessels, which directly contact with circulating substances. Numerous previous studies have demonstrated that HDL exert protective effects on maintaining endothelial integrity and enhance anti-inflammatory functions, etc. In this chapter, we introduced how HDL benefit endothelial functions. We summarized the function of HDL on endothelial cell, such as endothelial permeability, proliferation, migration, apoptosis, etc. In addition, we discussed the effects of HDL on classical endothelial functions, such as coagulation and vasodilation. Although HDL have huge effects on endothelial functions, lots of cardiovascular diseases such as atherosclerosis could not be fully prevented and treated. Thus, a further understanding of the relationship between HDL and endothelial cell is needed, which would create a potential therapeutic approach to cardiovascular diseases.
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http://dx.doi.org/10.1007/978-981-19-1592-5_3DOI Listing
May 2022

Utilizing network pharmacological analysis to investigate the key targets and mechanisms of kaempferol against oxaliplatin-induced neurotoxicity.

Toxicol Mech Methods 2022 May 15:1-9. Epub 2022 May 15.

Department of General Surgery, Xiangya Hospital, Central South University, Changsha Hunan, China.

This study investigated the pharmacological mechanism of kaempferol in the treatment of oxaliplatin-induced neuropathic pain by network pharmacological method and cells experiment. The kaempferol and disease target genes were obtained from several databases, including TCMSP, SwissTargetPrediction, GeneCards, and CTD. Then, the common target genes of drugs and diseases were obtained using Venny online tools. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional analyses were carried out to obtain the enriched molecular pathways associated with the kaempferol and disease. Finally, we constructed a neuropathic pain cell experiment to confirm the findings. 138 intersection targets were identified between targets of kaempferol and oxaliplatin-induced neurotoxicity. Enrichment analyses revealed that the IL-17 signaling pathway was associated with the therapeutic effects of kaempferol. Kaempferol down-regulated the mRNA expression levels of TNF-α, IL-6, and CCL2 in oxaliplatin-treated astrocytes. Our findings showed that kaempferol alleviated oxaliplatin-induced neurotoxicity via regulation of inflammation-related genes.
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http://dx.doi.org/10.1080/15376516.2022.2069531DOI Listing
May 2022

MR-Guided Microwave Ablation for Lung Malignant Tumor: A Single Center Prospective Study.

Front Oncol 2022 27;12:856340. Epub 2022 Apr 27.

Department of Interventional Radiology, First Affiliated Hospital of Fujian Medical University, Fuzhou, China.

Objectives: To prospectively investigate the feasibility and efficacy of MRI-guided MWA for lung malignant tumor in our single center.

Materials And Methods: 22 patients [mean age, 56.86 ± 13.05(23-73)years] with 23 malignant lung tumors were enrolled in the study. 21 patients had a single lesion and 1 patient had 2 lesions in the ipsilateral lung. The average maximum diameter of the lesion was 1.26 ± 0.65 (0.50-2.58)cm. Percutaneous MWA was guided by 1.5T MRI scanner using a MR-compatible microwave antenna to the target the lung lesions and ablation area was monitored intraoperatively by using a shielded MR-compatible microwave device and then follow-up.

Results: All patients were successfully treated under MR-guided MWA for lung tumors. Average operation time was 72.21 ± 24.99 (36-158) mins. T2WI signal intensity of the lesion gradually decreased over the course of MWA. The center of the ablated zones showed a short T1 and short T2 signals with the ring-like of long T1 and long T2 signals surrounded after immediately evaluation. No serious complications occurred. The average follow-up period was 12.89 ± 4.33 (2.0-19.6) months. Local recurrence occurred in one patient, representing a technical efficacy of 95.5% (21/22).

Conclusion: Magnetic resonance-guided microwave ablation for lung malignant tumor was feasible and demonstrated unique advantages in efficacy evaluation.
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http://dx.doi.org/10.3389/fonc.2022.856340DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092249PMC
April 2022

Corrigendum: Application of and Clinical Research on Enhanced Recovery After Surgery in Perioperative Care of Patients With Supratentorial Tumors.

Front Oncol 2022 28;12:863458. Epub 2022 Apr 28.

Department of Nursing, Ningbo First Hospital, Ningbo, China.

[This corrects the article DOI: 10.3389/fonc.2021.697699.].
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http://dx.doi.org/10.3389/fonc.2022.863458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097871PMC
April 2022

Aligning Student-Faculty Mentorship Expectations and Needs to Promote Professional Identity Formation in Undergraduate Medical Education.

J Med Educ Curric Dev 2022 Jan-Dec;9:23821205221096307. Epub 2022 May 11.

Department of Medicine, Duke University Hospital, Durham, NC, USA.

Background: During professional identity formation, medical students integrate their newly developing professional identities with their longstanding personal identities. Longitudinal mentorship has been shown to aid students in this process. Lack of clear relationship expectations among students and faculty is a barrier to effective longitudinal mentorship relationships.

Methods: A cross-sectional, survey-based study collected information about experiences from both students (mentees) and faculty (mentors). Surveys focused on collecting participants' attitudes and expectations regarding ideal and actual mentorship experiences. Descriptive statistics and Fisher's exact test analyses were used to compare the responses within and between students and faculty.

Results: A total of 234 faculty and 181 medical students completed the survey. There were 187 faculty respondents (79.9%) who had previously mentored students. Faculty who had versus had not previously mentored students differed significantly in their responses on the importance of mentors discussing aspects of their personal lives (71.1% vs. 54.3%, respectively,  = 0.0491), a quality valued by the majority of student respondents. As students progressed through medical school, they expressed increasing needs for personal mentorship and conversations regarding work/life integration and wellness (M1: 12.2%, M2: 18.8%, M3: 29.3%, M4: 51.7%). A minority of students (27% of M3 and 14.8% of M4 students) reported meeting faculty mentors through their clinical year experiences.

Conclusions: Faculty mentoring experience may improve student-faculty value alignment, which may in turn help to address student-identified needs pertaining to personal development and professional identity formation in medical school. By contrasting student-identified mentorship expectations with those of faculty at various stages of mentorship experience, this study lays the groundwork for the formation of more effective longitudinal mentorship programs.
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http://dx.doi.org/10.1177/23821205221096307DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102129PMC
May 2022

A --rearranged gastric mesenchymal tumor accompanied by gastritis cystica profunda and with probable benign behavior: a case report.

Transl Cancer Res 2022 Apr;11(4):959-966

Department of Pathology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Genomic rearrangements involving and the family of transcription factors are increasingly detected in an array of mesenchymal neoplasms, most of which are malignant. Gastritis cystica profunda (GCP) is a rare disease characterized by cystic dilatation of gastric glands into the submucosa and generally regarded as a precursor to tumor. Herein, we report a peculiar case in which an -rearranged gastric mesenchymal tumor was admixed with GCP in a 64-year-old woman. All layers of the gastric wall were invaded, although no lymph node or neural invasion, or tumoral vascular emboli was noted. The mass showed readily distinguishable epithelial and mesenchymal components. The epithelial component consisted mainly of glandular structures with some showing metaplastic growth. The epithelial cells showed focally atypical hyperchromatic nuclei, slightly eosinophilic cytoplasm, and infrequent mitosis. The mesenchymal component consisted of monomorphic, ovoid-shaped cells with scanty cytoplasm, regular nuclei, and rare mitotic figures. Immunohistochemically, the epithelial cells were uniformly positive for cytokeratins, and the mesenchymal neoplasm showed focal positivity for CD10, CD117 and CD56. An - fusion was identified with genomic profiling and confirmed with fluorescence in situ hybridization (FISH) in the tumor. Given the low mitotic activity, absence of nodal or distant spread and vascular or neural invasion, and disease-free status at 28-month follow-up, both lesions were likely benign. To our knowledge, this is the first to report an fusion in a gastric mesenchymal tumor with accompanying GCP.
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http://dx.doi.org/10.21037/tcr-21-2331DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9091036PMC
April 2022

Methyltransferase-like 3 Aggravates HCC Development via Mediating N6-Methyladenosine of Ubiquitin-Specific Protease 7.

J Oncol 2022 5;2022:6167832. Epub 2022 May 5.

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006 ., China.

We aimed to investigate the role of methyltransferase-like 3 (METTL3) in regulating HCC by mediating m6A level of ubiquitin-specific protease (USP7). METTL3 levels and m6A contents in HCC tissues and cells were detected. Potential correlations between METTL3 level and lymphatic metastasis, tumor size, tumor staging, and overall survival of HCC patients were analyzed. Moreover, its regulatory effects on proliferative, migratory, and invasive rates of HCC cells were examined. Potential methylation of USP7 in HCC was predicted using an online software, and the correlation between USP7 and METTL3 was assessed. METTL3 and m6A were increased both in HCC cells and tissues. High level of METTL3 was associated with the incidence of lymphatic metastasis, large tumor size, advanced tumor staging, and low overall survival of HCC. Silencing of METTL3 reduced proliferation, migration, and invasion rates. USP7 was predicted to have a methylation site regulated by METTL3. It was upregulated in HCC and associated with METTL3 level positively. USP7 silencing decreased proliferation, migration, and invasion rates of HCC cells. METTL3 promotes HCC to proliferate, migrate, and invade by regulating m6A methylation of USP7.
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http://dx.doi.org/10.1155/2022/6167832DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098324PMC
May 2022

UDP/P2Y contributes to enhancing LPS-induced acute lung injury by regulating neutrophil migration.

Cell Immunol 2022 Apr 30;376:104530. Epub 2022 Apr 30.

Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, China. Electronic address:

Neutrophils play a prominent role in the inflammatory response and are a critical factor in the pathogenesis of acute lung injury (ALI). Despite a deep understanding of neutrophil accumulation in the pulmonary microvasculature during the process of this disease, the regulatory mechanism of neutrophil recruitment remains unclear. This study aimed to explore the functions and signaling pathways of the purinergic receptor P2Y in mediating the innate immune response in ALI. P2Y-deficient mice, bone marrow chimeras, and neutrophilic chimeras were created in this work to explore the function of P2Y in ALI. The results indicated that the extracellular nucleotide UDP was released as a dangerous signal and activated P2Y to promote the inflammatory response and pulmonary damage during the process of ALI. P2Y deficiency may mitigate deterioration of this disease, including reduced ALI-related inflammatory factor release and immune cell invasion. Bone marrow and neutrophil chimeras and adoptive transfer in mice showed that P2Y expression on neutrophils contributed to neutrophil infiltration into lung tissues induced by UDP. Further work indicated that P2Y was involved in the neutrophil migration capability through the ErK signaling pathway by mediating the deformation of F-actin filaments and pseudopodia formation during cell recruitment to pulmonary tissue. Here, we provide evidence for the mechanism by which the purinergic receptor P2Y contributes to ALI development by regulating neutrophil infiltration into lung tissues. These data indicated that P2Y might be a potential therapeutic target for the treatment of this acute severe disease.
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http://dx.doi.org/10.1016/j.cellimm.2022.104530DOI Listing
April 2022

Effect of human follicle-stimulating hormone on immunomodulatory function of decidual mesenchymal stem cells by reducing interleukin-6 levels.

J Ovarian Res 2022 May 13;15(1):60. Epub 2022 May 13.

Reproductive Centre, Sanya Women and Children's Hospital Managed By Shanghai Children's Medical Center, 339 Yingbin Road, Sanya, 572000, China.

Objective: Women with an elevated basal FSH indicate diminished ovarian reserve and reduced oocyte and embryo numbers. DMSCs are likely to be involved in immune tolerance of pregnancy maintenance. We investigate the effect of follicle-stimulating hormones on the immunomodulatory functions of DMSCs.

Methods: DMSCs were primary cultured from decidual tissue. Pretreated DMSCs with mitomycin C, combined with CD4 T lymphocytes, DMSCs + CD4T co-culture system was established. Different physiological dose FSH (3 ng/ml,10 ng/ml,30 ng/ml,100 ng/ml) were used to co-culture system. Cytokines (IFN-γ, IL-2, IL-4, IL-6, IL-10, TNF-α) and other proteins (FSHR, MyD88) were measured.

Results: Compared with the control group (FSH (0 ng/mL) + CD4T + DMSCs), the FSH concentration was 10, 30, and 100 ng/ml, IL-6 levels were significantly reduced (P < 0.05). IL-6, MyD88 protein expression was remarkably decreased (P < 0.05).

Conclusion: FSH/FSHR could negatively regulate the immunosuppressive function of DMSCs by reducing secretion of IL-6 levels through MyD88 pathways, but upstream and downstream signalling pathways require further validation.
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http://dx.doi.org/10.1186/s13048-022-00993-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102716PMC
May 2022

Identification and validation of TNFRSF4 as a high-profile biomarker for prognosis and immunomodulation in endometrial carcinoma.

BMC Cancer 2022 May 13;22(1):543. Epub 2022 May 13.

Department of Pathology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Science, Beijing, 100730, China.

Background: The interaction between tumor microenvironment (TME) and tumors offers various targets in mounting anti-tumor immunotherapies. However, the prognostic biomarkers in endometrial carcinoma (EC) are still limited. Here, we aimed to analyze the TME features and identify novel prognostic biomarkers for EC.

Methods: ESTIMATE, CIBERSORT, protein-protein interaction (PPI) network, univariate and multivariate Cox regression, and functional enrichment analysis were performed to identify immune- and survival-related hub genes as well as possible molecular mechanisms. The limma package and deconvolution algorithm were adopted to estimate the abundance of tumor-infiltrating immune cells (TICs) and their relationship with the target gene. In the validation section, tissue microarrays (TMAs) of EC and multiplex immunohistochemistry (m-IHC) were evaluated to validate the expression of TNFRSF4, and its correlation with immune markers, including CD4, CD8, and FOXP3. Besides, the receiver operating characteristic (ROC) curve was plotted to determine the diagnostic performance of TNFRSF4, CD4, CD8, and FOXP3 in EC.

Results: Two genes, TNFRSF4 and S1PR4, were screened out from 386 intersection differential expression genes (DEGs) shared by ImmuneScore and StromalScore in EC. Highlighted by TNFRSF4, we found that it was not only positively correlated with the TICs (mainly CD4 T cells, CD8 T cells, and Tregs) but significantly related to the prognosis in patients of EC, both verified by data from The Cancer Genome Altas (TCGA)-EC database and clinical samples. At the same time, the expression trend of TNFRSF4 was further confirmed by an integrated meta-analysis based on six microarrays from the Gene Expression Omnibus database (GEO).

Conclusions: Collectively, TNFRSF4, a previously unrecognized key player in EC, could serve as a potential biomarker for prognosis prediction and immunomodulation of EC.
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http://dx.doi.org/10.1186/s12885-022-09654-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107201PMC
May 2022

Glioblastoma: Changing concepts in the WHO CNS5 classification.

Indian J Pathol Microbiol 2022 May;65(Supplement):S24-S32

Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri, USA.

Glioblastoma is the most common malignant central nervous system (CNS) tumor in adults. Acute common clinical symptoms include headache, seizure, behavior changes, focal neurological deficits, and signs of increased intracranial pressure. The classic MRI finding of glioblastoma is an irregularly shaped, rim-enhancing or ring-enhancing lesion with a central dark area of necrosis. This constellation of features correlates with microscopic findings of tumor necrosis and microvascular proliferation. Besides these common features, several well-recognized histological subtypes include giant cell glioblastoma, granular cell glioblastoma, gliosarcoma, glioblastoma with a primitive neuronal component, small cell glioblastoma, and epithelioid glioblastoma. While glioblastoma was historically classified as isocitrate dehydrogenase (IDH)-wildtype and IDH-mutant groups, the Consortium to Inform Molecular and Practical Approaches to CNS Tumor Taxonomy (cIMPACT-NOW) and the fifth edition of the WHO Classification of Tumors of the Central Nervous System clearly updated the nomenclature to reflect glioblastoma to be compatible with wildtype IDH status only. Therefore, glioblastoma is now defined as "a diffuse, astrocytic glioma that is IDH-wildtype and H3-wildtype and has one or more of the following histological or genetic features: microvascular proliferation, necrosis, Telomerase reverse transcriptase promoter mutation, Epidermal growth factor receptor gene amplification, +7/-10 chromosome copy-number changes (CNS WHO grade 4)."
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http://dx.doi.org/10.4103/ijpm.ijpm_1109_21DOI Listing
May 2022

Du13 encodes a C H zinc-finger protein that regulates Wx pre-mRNA splicing and microRNA biogenesis in rice endosperm.

Plant Biotechnol J 2022 May 13. Epub 2022 May 13.

State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, China.

Amylose content is a crucial physicochemical property responsible for the eating and cooking quality of rice (Oryza sativa L.) grain and is mainly controlled by the Waxy (Wx) gene. Previous studies have identified several Dull genes that modulate the expression of the Wx allele in japonica rice by affecting the splicing efficiency of the Wx pre-mRNA. Here, we uncover dual roles for a novel Dull gene in pre-mRNA splicing and microRNA processing. We isolated the dull mutant, du13, with a dull endosperm and low amylose content. Map-based cloning showed that Du13 encodes a C H zinc-finger protein. Du13 coordinates with the nuclear cap-binding complex to regulate the splicing of Wx transcripts in rice endosperm. Moreover, Du13 also regulates alternative splicing of other protein-coding transcripts and affects the biogenesis of a subset of microRNAs. Our results reveal an evolutionarily conserved link between pre-mRNA splicing and microRNA biogenesis in rice endosperm. Our findings also provide new insights into the functions of Dull genes in rice and expand our knowledge of microRNA biogenesis in monocots.
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http://dx.doi.org/10.1111/pbi.13821DOI Listing
May 2022

Assessment of the prognostic value of in clear cell renal cell carcinoma: a bioinformatics analysis.

Transl Androl Urol 2022 Apr;11(4):509-518

Department of Pharmacy, Traditional Chinese Medical Hospital of Zhuji, Zhuji, China.

Background: Clear cell renal cell carcinoma (ccRCC) is one of the most common urological malignancies, and once metastasis occurs, it often has a poor prognosis and lacks effective treatment. Therefore, there is an urgent need to screen some new biomarkers and explore their molecular mechanisms to improve the early clinical diagnosis and targeted therapy of ccRCC. (SPARC/osteonectin, CWCV and Kazal-like domains proteoglycan 1) is a conserved multi-domain proteoglycan that plays an important role in the development of multiple cancer types; however, its prognostic value in ccRCC has not been investigated. The study of the prognostic value of in ccRCC is a good complement to the study of ccRCC biomarkers.

Methods: Databases of this study included Oncomine, Kaplan-Meier Plotter, GEPIA, GeneMANIA, cBioPortal, and TIMER. Student's -test was used to analyze the differences in expression in ccRCC tissues compared with tumor-adjacent normal tissues. Kaplan-Meier curves for survival analysis were used to assess the correlation between the expression of and the prognostic outcomes. Correlation module drew the expression scatterplots between and immune cell infiltration in ccRCC, together with the Spearman's rho value and estimated statistical significance.

Results: The mRNA expression was significantly higher in ccRCC tissues (mean expression ± SD: 920.2±195.2) than in normal tissues (mean expression ± SD: 358.4±29.1, P=0.008), and high expression significantly and positively correlated with the pathological stage of ccRCC patients (F value =10.2, P<0.001). Higher expression of was also associated with significantly shorter overall survival (OS) and disease-free survival (DFS) in ccRCC patients (GEPIA: P=0.046, P<0.001, respectively; Kaplan-Meier Plotter: P=0.002, P=0.0022, respectively). The function of SPOCK1 is mainly related to tumor development and extracellular matrix remodeling, and it may participate in the epithelial-mesenchymal transition process. expression significantly and positively correlated with infiltration of several immune cells in ccRCC, including cancer-associated fibroblasts (CAFs) (Rho =0.333, P=2.16×10), tumor-associated macrophages (TAMs) (Rho =0.18, P=1.02×10), and tumor-associated neutrophils (TANs) (Rho =0.165, P=3.83×10). Conversely, there was a significant and negative correlation between expression and infiltration of CD4 T cells (Rho =-0.113, P=0.015).

Conclusions: may be a potential prognostic biomarker in ccRCC.
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http://dx.doi.org/10.21037/tau-22-161DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9085920PMC
April 2022

An artificial intelligent difficulty scoring system in stones removal during endoscopic retrograde cholangiopancreatography: a prospective validation.

Endoscopy 2022 May 12. Epub 2022 May 12.

Key Laboratory of Hubei Province for Digestive System Disease, Renmin Hospital of Wuhan University, Wuhan, China.

Background: A computer-assisted (CAD) system was developed to assess and score the technical difficulty factors to classify the technical difficult degree of common bile duct (CBD) stones removal during Endoscopic Retrograde Cholangiopancreatography (ERCP). Subsequently, the efficacy of the CAD system was assessed through a multi-center, prospective, observational study.

Method: All patients who met the inclusion criteria were included in this study. Based on cholangiogram images, the CAD system analyzed the extent of difficulty in stone removal and classified it into difficult and easy groups. Further, we compared the differences in clinical endpoints, including attempts of stone extraction, stone extraction time, the whole operation time, and stone clearance rates between the two groups.

Results: In total, 173 patients with CBD stones from three hospitals were included in the study. Consequently, the difficult group had more extraction attempts (7.20 vs. 4.20, P<0.001), more frequent machine lithotripsy (30.43% vs. 7.09%, P<0.001), longer stone extraction time (16.59min vs. 7.65min, P<0.001), lower single-session stone clearance rate (73.91 % vs. 94.49 %, P<0.001) and lower total stone clearance rate (87.13% vs. 97.64%, P=0.019) compared to the easy group.

Conclusion: The CAD system can effectively assess and classify the extent of technical difficulty in endoscopic stone extraction during ERCP. Besides, it automatically provides a quantitative evaluation of CBD and stones, which in turn would help endoscopists to apply suitable procedures and interventional methods to minimize the possible risks of endoscopic stone removal.
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http://dx.doi.org/10.1055/a-1850-6717DOI Listing
May 2022

Comparison of a preoperative MR-based recurrence risk score versus the postoperative score and four clinical staging systems in hepatocellular carcinoma: a retrospective cohort study.

Eur Radiol 2022 May 13. Epub 2022 May 13.

Department of Radiology, West China Hospital, Sichuan University, No. 37, GUOXUE Alley, Chengdu, 610041, Sichuan, China.

Objectives: To establish a risk score integrating preoperative gadoxetic acid-enhanced magnetic resonance imaging (EOB-MRI) and clinical parameters to predict recurrence after hepatectomy for patients with hepatocellular carcinoma (HCC) and to compare its performance with that of a postoperative score and four clinical staging systems.

Methods: Consecutive patients with surgically confirmed HCC who underwent preoperative EOB-MRI between July 2015 and November 2020 were retrospectively included. Two recurrence risk scores, one incorporating only preoperative variables and the other incorporating all preoperative and postoperative variables, were constructed via Cox regression models.

Results: A total of 214 patients (derivation set, n = 150; test set, n = 64) were included. Six preoperative variables, namely tumor number, infiltrative appearance, corona enhancement, alpha-fetoprotein (AFP) level, aspartate aminotransferase (AST) level, and sex, were independently associated with recurrence. After adding postoperative features, microvascular invasion and tumor differentiation were additional significant variables in lieu of corona enhancement and AFP level. Using the above variables, the preoperative score achieved a C-index of 0.741 on the test set, which was comparable with that of the postoperative score (0.729; p = 0.235). The preoperative score yielded a larger time-dependent area under the receiver operating characteristic curve at 1 year (0.844) than three existing systems (0.734-0.742; p < 0.05 for all). Furthermore, the preoperative score stratified patients into two prognostically distinct risk strata with low and high risks of recurrence (p < 0.001).

Conclusion: The preoperative score integrating EOB-MRI features, AFP and AST levels, and sex improves recurrence risk estimation in HCC.

Key Points: • The preoperative risk score incorporating three EOB-MRI findings, AFP and AST levels, and sex achieved comparable performance with that of the postoperative score for predicting recurrence after hepatectomy in patients with HCC. • Two risk strata with low and high risks of recurrence were obtained based on the preoperative score. • The preoperative score may help tailor pretreatment decision-making and facilitate candidate selection for adjuvant clinical trials.
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http://dx.doi.org/10.1007/s00330-022-08811-6DOI Listing
May 2022

Artificial Intelligence-Based Automated Treatment Planning of Postmastectomy Volumetric Modulated Arc Radiotherapy.

Front Oncol 2022 25;12:871871. Epub 2022 Apr 25.

Department of Radiation Ocology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Breast Cancer Prevention and Therapy, Tianjin Medical University, Ministry of Education, Tianjin, China.

As a useful tool, artificial intelligence has surpassed human beings in many fields. Artificial intelligence-based automated radiotherapy planning strategies have been proposed in lots of cancer sites and are the future of treatment planning. Postmastectomy radiotherapy (PMRT) decreases local recurrence probability and improves overall survival, and volumetric modulated arc therapy (VMAT) has gradually become the mainstream technique of radiotherapy. However, there are few customized effective automated treatment planning schemes for postmastectomy VMAT so far. This study investigated an artificial intelligence based automated planning using the MD Anderson Cancer Center AutoPlan (MDAP) system and Pinnacle treatment planning system (TPS), to effectively generate high-quality postmastectomy VMAT plans. In this study, 20 patients treated with PMRT were retrospectively investigated, including 10 left- and 10 right-sided postmastectomy patients. Chest wall and the supraclavicular, subclavicular, and internal mammary regions were delineated as target volume by radiation oncologists, and 50 Gy in 25 fractions was prescribed. Organs at risk including heart, spinal cord, left lung, right lung, and lungs were also contoured. All patients were planned with VMAT using 2 arcs. An optimization objective template was summarized based on the dose of clinical plans and requirements from oncologists. Several treatment planning parameters were investigated using an artificial intelligence algorithm, including collimation angle, jaw collimator mode, gantry spacing resolution (GSR), and number of start optimization times. The treatment planning parameters with the best performance or that were most preferred were applied to the automated treatment planning method. Dosimetric indexes of automated treatment plans (autoplans) and manual clinical plans were compared by the paired t-test. The jaw tracking mode, 2-degree GSR, and 3 rounds of optimization were selected in all the PMRT autoplans. Additionally, the 350- and 10-degree collimation angles were selected in the left- and right-sided PMRT autoplans, respectively. The uniformity index and conformity index of the planning target volume, mean heart dose, spinal cord D, mean lung dose, and V and V of the lung of autoplans were significantly better compared with the manual clinical plans. An artificial intelligence-based automated treatment planning method for postmastectomy VMAT has been developed to ensure plan quality and improve clinical efficiency.
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http://dx.doi.org/10.3389/fonc.2022.871871DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9084926PMC
April 2022

Probiotics improves abnormal behavior and hippocampal injury in pregnant-stressed offspring rats.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2022 Apr;47(4):443-452

Department of Anatomy and Neurobiology, School of Basic Medical Science, Central South University, Changsha 410013.

Objectives: During pregnancy, pregnant women are prone to stress reactions due to external stimuli, affecting their own health and fetal development. At present, there is no good treatment for the stress reactions from pregnant women during pregnancy. This study aims to explore the effect of probiotics on abnormal behavior and hippocampal injury in pregnant stressed offspring.

Methods: SD pregnant rats were divided into a control group, a stress group, and a probiotics group, with 6 rats in each group. The control group was untreated; the stress group was given restraint stress on the 15th-20th day of pregnancy; the probiotics group was given both bifidobacterium trisporus capsules and restraint stress on the 15th-20th day of pregnancy, and the offspring continued to be fed with probiotics until 60 days after birth (P60). The offspring rats completed behavioral tests such as the open field test, the elevated plus maze test, the new object recognition test, and the barnes maze test at 60-70 d postnatally. Nissl's staining was used to reflect the injury of hippocampal neurons; immunohistochemical staining was used to detect the expression of microglia marker ionized calcium binding adapter molecule 1 (IBA-1) which can reflect microglia activation; ELISA was used to detect the content of plasma TNF-α and IL-1β; Western blotting was used to detect the expression of Bax, Bcl-2, and caspase-3.

Results: The retention time of offspring rats in the stress group in the central area of the open field was significantly less than that in the control group (<0.01), and the retention time of offspring rats in the probiotic group in the central area of the open field was significantly more than that in the stress group (<0.05). The offspring rats in the stress group stayed in the open arm for a shorter time than the control group (<0.05) and entered the open arm less often than the control group (<0.01); the offspring rats in the probiotic group stayed in the open arm for a longer time than the stress group and entered the open arm more often than the stress group (both <0.05). The discrimination ratio for new to old objects in the offspring rats of the stress group was significantly lower than that of the control group (<0.01), and the discrimination ratio for new to old objects in the offspring rats of the probiotic group was significantly higher than that of the stress group (<0.05). The offspring rats in the stress group made significantly more mistakes than the control group (<0.05), and the offspring rats in the probiotic group made significantly fewer mistakes than the stress group (<0.05). Compared with the control group, the numbers of Nissl bodies in CA1, CA3, and DG area were significantly reduced in the offspring rats of the stress group (all <0.001), the number of activated microglia in DG area of hippocampus was significantly increased (<0.01), the contents of TNF-α and IL-1β in peripheral blood were significantly increased (<0.05 or <0.01), the protein expression level of Bcl-2 was significantly down-regulated, and the protein expression levels of Bax and caspase-3 were significantly up-regulated (all <0.001). Compared with the stress group, the numbers of Nissl bodies in CA1, CA3, and DG area were significantly increased in the probiotic group offspring rats (<0.001, <0.01, <0.05), the number of activated microglia in the DG area of hippocampus was significantly reduced (<0.05), and the TNF-α and IL-1β levels in peripheral blood were significantly decreased (both <0.05), the protein expression level of Bcl-2 was significantly up-regulated, and the protein expression levels of Bax and caspase-3 were significantly down-regulated (all <0.001).

Conclusions: Probiotic intervention partially ameliorated anxiety and cognitive impairment in rats offspring of pregnancy stress, and the mechanism may be related to increasing the number of neurons, inhibiting the activation of hippocampal microglia, and reducing inflammation and apoptosis.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2022.210231DOI Listing
April 2022

Long-term exposure to air pollution and mortality in a Danish nationwide administrative cohort study: Beyond mortality from cardiopulmonary disease and lung cancer.

Environ Int 2022 Apr 12;164:107241. Epub 2022 Apr 12.

National Institute of Public Health, University of Southern Denmark, Copenhagen, Denmark.

Background: The association between long-term exposure to air pollution and mortality from cardiorespiratory diseases is well established, yet the evidence for other diseases remains limited.

Objectives: To examine the associations of long-term exposure to air pollution with mortality from diabetes, dementia, psychiatric disorders, chronic kidney disease (CKD), asthma, acute lower respiratory infection (ALRI), as well as mortality from all-natural and cardiorespiratory causes in the Danish nationwide administrative cohort.

Methods: We followed all residents aged ≥ 30 years (3,083,227) in Denmark from 1 January 2000 until 31 December 2017. Annual mean concentrations of fine particulate matter (PM), nitrogen dioxide (NO), black carbon (BC), and ozone (warm season) were estimated using European-wide hybrid land-use regression models (100 m × 100 m) and assigned to baseline residential addresses. We used Cox proportional hazard models to evaluate the association between air pollution and mortality, accounting for demographic and socioeconomic factors. We additionally applied indirect adjustment for smoking and body mass index (BMI).

Results: During 47,023,454 person-years of follow-up, 803,881 people died from natural causes. Long-term exposure to PM (mean: 12.4 µg/m), NO (20.3 µg/m), and/or BC (1.0 × 10/m) was statistically significantly associated with all studied mortality outcomes except CKD. A 5 µg/m increase in PM was associated with higher mortality from all-natural causes (hazard ratio 1.11; 95% confidence interval 1.09-1.13), cardiovascular disease (1.09; 1.07-1.12), respiratory disease (1.11; 1.07-1.15), lung cancer (1.19; 1.15-1.24), diabetes (1.10; 1.04-1.16), dementia (1.05; 1.00-1.10), psychiatric disorders (1.38; 1.27-1.50), asthma (1.13; 0.94-1.36), and ALRI (1.14; 1.09-1.20). Associations with long-term exposure to ozone (mean: 80.2 µg/m) were generally negative but became significantly positive for several endpoints in two-pollutant models. Generally, associations were attenuated but remained significant after indirect adjustment for smoking and BMI.

Conclusion: Long-term exposure to PM, NO, and/or BC in Denmark were associated with mortality beyond cardiorespiratory diseases, including diabetes, dementia, psychiatric disorders, asthma, and ALRI.
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http://dx.doi.org/10.1016/j.envint.2022.107241DOI Listing
April 2022

Distributed Stochastic Gradient Tracking Algorithm With Variance Reduction for Non-Convex Optimization.

IEEE Trans Neural Netw Learn Syst 2022 May 10;PP. Epub 2022 May 10.

This article proposes a distributed stochastic algorithm with variance reduction for general smooth non-convex finite-sum optimization, which has wide applications in signal processing and machine learning communities. In distributed setting, a large number of samples are allocated to multiple agents in the network. Each agent computes local stochastic gradient and communicates with its neighbors to seek for the global optimum. In this article, we develop a modified variance reduction technique to deal with the variance introduced by stochastic gradients. Combining gradient tracking and variance reduction techniques, this article proposes a distributed stochastic algorithm, gradient tracking algorithm with variance reduction (GT-VR), to solve large-scale non-convex finite-sum optimization over multiagent networks. A complete and rigorous proof shows that the GT-VR algorithm converges to the first-order stationary points with O(1/k) convergence rate. In addition, we provide the complexity analysis of the proposed algorithm. Compared with some existing first-order methods, the proposed algorithm has a lower O(PMε⁻¹) gradient complexity under some mild condition. By comparing state-of-the-art algorithms and GT-VR in numerical simulations, we verify the efficiency of the proposed algorithm.
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http://dx.doi.org/10.1109/TNNLS.2022.3170944DOI Listing
May 2022

Effects of Intravenous Analgesia Using Tramadol on Postoperative Depression State and Sleep Quality in Women Undergoing Abdominal Endoscopic Surgery: A Randomized Controlled Trial.

Drug Des Devel Ther 2022 2;16:1289-1300. Epub 2022 May 2.

Department of Anesthesiology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, People's Republic of China.

Purpose: This study aimed to explore the effects of intravenous analgesia using tramadol on postoperative depression, anxiety, and sleep in women undergoing abdominal endoscopic surgery.

Patients And Methods: Two hundred female patients (100 in each group) who underwent abdominal endoscopic surgery were recruited to randomly receive intravenous analgesia with sufentanil combined with tramadol (tramadol group) or sufentanil (control group). The primary outcome was the incidence of postoperative depression, which was assessed at 1, 2, and 3 days after surgery using the 13-item Beck Depression Inventory. The secondary outcomes were the incidence of anxiety and sleep quality, which were assessed using the 20-item Self-Rating Anxiety Scale and Richards-Campbell Sleep Questionnaire.

Results: The incidence of depression (Beck depression scale≥4) during the 3-day follow-up in the control group was 51%, which was significantly higher than that in the tramadol group of 28% (relative risk [RR]=0.55; 95% confidence interval [CI], 0.38-0.79; =0.001). No difference was found in the incidence of anxiety state (Self-Rating Anxiety Scale≥40) between the tramadol and control groups (7%vs 5%; RR=1.40; 95% CI, 0.46-4.25; =0.552). All of the Richards-Campbell sleep scales of patients in the tramadol group at 1 (77.4±15.2 vs 64.2±20.1, <0.001), 2 (84.1±14.9 vs 71.8±18.8, <0.001), and 3 days (87.0±12.2 vs 70.3±21.0, <0.001) after surgery were higher than those in the control group.

Conclusion: Intravenous analgesia using tramadol can effectively improve the postoperative depression and sleep status of women undergoing abdominal endoscopic surgery. Tramadol is recommended for use in postoperative analgesia when improving postoperative mood, and sleep is needed in clinical practice.
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http://dx.doi.org/10.2147/DDDT.S357773DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9075899PMC
May 2022

MiR-144 regulates adipogenesis by mediating formation of C/EBPα-FOXO1 protein complex.

Biochem Biophys Res Commun 2022 Apr 28;612:126-133. Epub 2022 Apr 28.

College of Animal Science & Technology, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China. Electronic address:

CeRNA effect was an important regulation mode of miRNA mediated bio-activities, however, most of the researches of ceRNA were on ncRNAs synergetic with mRNAs, the exploration of ceRNA effect regulated mRNA interaction was still lack of. Besides, C/EBPα was one of the most crucial adipogenic regulators, which has been demonstrated to form a protein complex with FOXO1 to mediate AdipoQ expression. So that, we try to explore whether the ceRNA effect mediated the interaction of C/EBPα and FOXO1, and identified the key miRNAs of their ceRNA effect. In this paper, we found the ceRNA effect of C/EBPα and FOXO1 mediated their protein complex formation, furthermore regulated its transcriptional role for AdipoQ, thereby influencing pre-adipocytes adipogenesis. More importantly, we demonstrated that the miR-144 was the decisive factor that mediated the ceRNA effect of C/EBPα and FOXO1 to influence AdipoQ, thus regulated pre-adipocytes adipogenesis. This research will provide a new supplementary idea of the miRNA role in mediating coding RNA interaction that regulates pre-adipocyte adipogenesis.
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http://dx.doi.org/10.1016/j.bbrc.2022.04.093DOI Listing
April 2022

Metabolic perturbations and health impact from exposure to a combination of multiple harmful Maillard reaction products on Sprague-Dawley rats.

Food Funct 2022 May 6. Epub 2022 May 6.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.

The present study aimed to investigate the metabolic perturbations and health impact of the co-accumulation of Maillard reaction products (MRPs), including acrylamide, harmane, and -(carboxymethyl)lysine (CML), serum biochemical and histopathological examinations as well as metabolomic analysis. Sprague-Dawley rats were treated with acrylamide (2 mg per kg body weight [bw]), harmane (1 mg per kg bw), CML (2 mg per kg bw), and combinations of these MRPs. Harmane did not cause adverse effects on the health of rats, whereas acrylamide and CML resulted in significantly ( < 0.05) decreased insulin sensitivity (HOMA-IR > 1), increased oxidative stress levels, and pathological injuries to the pancreas, liver, and gastrocnemius. Owing to the antioxidant and anti-diabetic activities of harmane, the effects of the combination of the MRPs on oxidative stress levels, blood glucose metabolism, and pathological injuries to the pancreas and gastrocnemius were relieved. However, new health problems, including pathological injury of the kidneys and increased cancer risk, were observed. Metabolomic analysis revealed that this may be related to the effects of MRPs on the arginine biosynthesis pathway, which resulted in the abnormal metabolism of fumaric acid and the tricarboxylic acid cycle. These results indicated that the mechanisms of the combined effect of MRPs and their effects on health cannot be predicted from the effects of individual MRPs.
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http://dx.doi.org/10.1039/d2fo00143hDOI Listing
May 2022

A Stimuli-Responsive Small-Molecule Metal-Carrying Prochelator: A Novel Prodrug Design Strategy for Metal Complexes.

Angew Chem Int Ed Engl 2022 May 5:e202203500. Epub 2022 May 5.

School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, China.

Selective activation of prodrugs is an important approach to reduce the side effects of disease treatment. We report a prodrug design concept for metal complexes, termed "metal-carrying prochelator", which can co-carry a metal ion and chelator within a single small-molecule compound and remain inert until it undergoes a specifically triggered intramolecular chelation to synthesize a bioactive metal complex in situ for targeted therapy. As a proof-of-concept, we designed a H O -responsive small-molecule prochelator, DPBD, based on the strong chelator diethyldithiocarbamate (DTC) and copper. DPBD can carry Cu (DPBD-Cu) and respond to elevated H O levels in tumor cells by releasing DTC, which rapidly chelates Cu from DPBD-Cu affording a DTC-copper complex with high cytotoxicity, realizing potent antitumor efficacy with low systemic toxicity. Thus, with its unique intramolecularly triggered activation mechanism, this concept based on a small-molecule metal-carrying prochelator can help in the prodrug design of metal complexes.
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http://dx.doi.org/10.1002/anie.202203500DOI Listing
May 2022

Antibiotic-Like Activity of Atomic Layer Boron Nitride for Combating Resistant Bacteria.

ACS Nano 2022 May 5. Epub 2022 May 5.

State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou Medical College, Soochow University, Suzhou 215123, Jiangsu, China.

The global rise of antimicrobial resistance (AMR) that increasingly invalidates conventional antibiotics has become a huge threat to human health. Although nanosized antibacterial agents have been extensively explored, they cannot sufficiently discriminate between microbes and mammals, which necessitates the exploration of other antibiotic-like candidates for clinical uses. Herein, two-dimensional boron nitride (BN) nanosheets are reported to exhibit antibiotic-like activity to AMR bacteria. Interestingly, BN nanosheets had AMR-independent antibacterial activity without triggering secondary resistance in long-term use and displayed excellent biocompatibility in mammals. They could target key surface proteins (, FtsP, EnvC, TolB) in cell division, resulting in impairment of Z-ring constriction for inhibition of bacteria growth. Notably, BN nanosheets had potent antibacterial effects in a lung infection model by (AMR), displaying a 2-fold increment of survival rate. Overall, these results suggested that BN nanosheets could be a promising nano-antibiotic to combat resistant bacteria and prevent AMR evolution.
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http://dx.doi.org/10.1021/acsnano.1c11353DOI Listing
May 2022

GraphTGI: an attention-based graph embedding model for predicting TF-target gene interactions.

Brief Bioinform 2022 May;23(3)

College of Computer Science and Software Engineering, ShenZhen University, 3688 Nanhai Avenue, Shenzhen, China.

Motivation: Interaction between transcription factor (TF) and its target genes establishes the knowledge foundation for biological researches in transcriptional regulation, the number of which is, however, still limited by biological techniques. Existing computational methods relevant to the prediction of TF-target interactions are mostly proposed for predicting binding sites, rather than directly predicting the interactions. To this end, we propose here a graph attention-based autoencoder model to predict TF-target gene interactions using the information of the known TF-target gene interaction network combined with two sequential and chemical gene characters, considering that the unobserved interactions between transcription factors and target genes can be predicted by learning the pattern of the known ones. To the best of our knowledge, the proposed model is the first attempt to solve this problem by learning patterns from the known TF-target gene interaction network.

Results: In this paper, we formulate the prediction task of TF-target gene interactions as a link prediction problem on a complex knowledge graph and propose a deep learning model called GraphTGI, which is composed of a graph attention-based encoder and a bilinear decoder. We evaluated the prediction performance of the proposed method on a real dataset, and the experimental results show that the proposed model yields outstanding performance with an average AUC value of 0.8864 +/- 0.0057 in the 5-fold cross-validation. It is anticipated that the GraphTGI model can effectively and efficiently predict TF-target gene interactions on a large scale.

Availability: Python code and the datasets used in our studies are made available at https://github.com/YanghanWu/GraphTGI.
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http://dx.doi.org/10.1093/bib/bbac148DOI Listing
May 2022

Inhibition of TFEB promotes tumor-educated dendritic cells activation to enhance antitumor immune responses.

Mol Immunol 2022 Apr 30;147:30-39. Epub 2022 Apr 30.

Medical Research Center, The Affiliated Hospital of Nanjing Medical University, Changzhou No.2 People's Hospital, Changzhou 213003, China; Oncology Institute, The Affiliated Hospital of Nanjing Medical University, Changzhou No.2 People's Hospital, Changzhou 213003, China. Electronic address:

Tumors can induce the generation and accumulation of immunosuppressive cells in -the tumor microenvironment (TME). Among them, tumor-educated dendritic cells (TEDCs) involved in tolerance induction contribute greatly to the progression of tumors. However, the mechanisms governing the immunosuppressive function of dendritic cells in the TME are unclear. In this study, we found that the expression of transcription factor EB (TFEB) was significantly increased in TEDCs induced by cancer cell supernatant. TFEB knockdown significantly promoted the differentiation and maturation of TEDCs, with upregulated expression of CD11c and costimulatory molecules (CD86 and MHC-II) but reduced expression of the inhibitory molecule PD-L1, and enhanced their ability to induce Th1 proliferation and differentiation. Moreover, TEDCs with TFEB knockdown significantly reduced tumor growth with increased infiltration of CD11cMHC-II dendritic cells and effector T cells in tumor masses, thus leading to a delay in tumor progression. These findings demonstrate a critical role of TFEB in regulating the immunosuppression of TEDCs, with potential implications as an antitumor immune therapeutic approach.
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http://dx.doi.org/10.1016/j.molimm.2022.04.011DOI Listing
April 2022

Correction: the characteristics of video capsule endoscopy in pediatric Henoch-Schönlein purpura with gastrointestinal symptoms.

Pediatr Rheumatol Online J 2022 May 2;20(1):33. Epub 2022 May 2.

Department of gastroenterology, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, 3333 Bin Sheng Road, Hangzhou, 310052, Zhejiang Province, China.

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http://dx.doi.org/10.1186/s12969-022-00683-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9063330PMC
May 2022
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