Publications by authors named "Jialin Li"

199 Publications

Asiatic acid inhibits angiogenesis and vascular permeability through the VEGF/VEGFR2 signaling pathway to inhibit the growth and metastasis of breast cancer in mice.

Phytother Res 2021 Sep 19. Epub 2021 Sep 19.

First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China.

Anti-angiogenic medicines have been evaluated as anticancer therapies, however, their use remains limited in clinical practice due to associated adverse effects. Asiatic acid (AA) is known to have broad-spectrum anticancer properties, however, its effects on angiogenesis in breast cancer remain to be fully established. In this study, we analyzed the inhibitory effects of AA on angiogenesis using human umbilical vein endothelial cells (HUVECs) cultured in vitro and on the growth and metastasis of a subcutaneous breast cancer 4T1 tumor model and a lung metastasis model in vivo. AA significantly inhibited HUVECs proliferation, migration, and tube formation in vitro. In vivo, AA significantly reduced the microvascular density and blood vascular permeability in breast cancer tumors and inhibited growth and lung metastasis. AA inhibited the expression of vascular endothelial growth factor (VEGF) in HUVECs and subsequently downregulated the phosphorylation of vascular endothelial growth factor receptor 2 (VEGFR2) and its downstream target proteins including ERK1/2, Src, and FAK. These results indicate that AA significantly inhibits angiogenesis and blood vessel permeability through the VEGF/VEGFR2 signal axis to inhibit the growth and metastasis of breast cancer. Our data strongly demonstrate the potential applications of AA in the treatment of breast cancer.
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http://dx.doi.org/10.1002/ptr.7292DOI Listing
September 2021

α-Mangostin Induces Apoptosis and Inhibits Metastasis of Breast Cancer Cells via Regulating RXRα-AKT Signaling Pathway.

Front Pharmacol 2021 30;12:739658. Epub 2021 Aug 30.

School of Pharmaceutical Sciences, Xiamen University, Xiamen, China.

Mangostin, which has the function of anti-inflammatory, antioxidant, and anticancer, etc, is one of the main active ingredients of the hull of the mangosteen. The main objective of the study was to elucidate its anti-cancer function and possible mechanism. α-Mangostin was separated and structurally confirmed. MTT method was used to check the effect of mangostin on breast cancer cell proliferation. Then the effect of α-Mangostin on the transcriptional activity of RXRα was tested by dual-luciferase reporter gene assay. And Western blot (WB) was used to detect the expression of apoptosis-related proteins or cell cycle-associated proteins after treatment. Also, this study was to observe the effects of α-Mangostin on the invasion of breast cancer cell line MDA-MB-231. α-Mangostin regulates the downstream effectors of the PI3K/AKT signaling pathway by degrading RXRα/tRXRα. α-Mangostin can trigger PARP cleavage and induce apoptosis, which may be related to the induction of upregulated BAX expression and downregulation of BAD and cleaved caspase-3 expression in MDA-MB-231 cells through blockade of AKT signaling. The experiments verify that α-Mangostin have evident inhibition effects of invasion and metastasis of MDA-MB-231 cells. Cyclin D1 was involved in the anticancer effects of α-Mangostin on the cell cycle in MDA-MB-231 cells. α-Mangostin induces apoptosis, suppresses the migration and invasion of breast cancer cells through the PI3K/AKT signaling pathway by targeting RXRα, and cyclin D1 has involved in this process.
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http://dx.doi.org/10.3389/fphar.2021.739658DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8444262PMC
August 2021

Fecal multi-omics analysis reveals diverse molecular alterations of gut ecosystem in COVID-19 patients.

Anal Chim Acta 2021 Oct 26;1180:338881. Epub 2021 Jul 26.

Guangdong Provincial Key Laboratory of Biomedical Imaging and Guangdong Provincial Engineering Research Center of Molecular Imaging, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, 519000, China. Electronic address:

Gut ecosystem has profound effects on host physiology and health. Gastrointestinal (GI) symptoms were frequently observed in patients with COVID-19. Compared with other organs, gut antiviral response can result in more complicated immune responses because of the interactions between the gut microbiota and host immunity. However, there are still large knowledge gaps in the impact of COVID-19 on gut molecular profiles and commensal microbiome, hindering our comprehensive understanding of the pathogenesis of SARS-CoV-2 and the treatment of COVID-19. We performed longitudinal stool multi-omics profiling to systemically investigate the molecular phenomics alterations of gut ecosystem in COVID-19. Gut proteomes of COVID-19 were characterized by disturbed immune, proteolysis and redox homeostasis. The expression and glycosylation of proteins involved in neutrophil degranulation and migration were suppressed, while those of proteases were upregulated. The variable domains of Ig heavy chains were downregulated and the overall glycosylation of IgA heavy chain constant regions, IgGFc-binding protein, and J chain were suppressed with glycan-specific variations. There was a reduction of beneficial gut bacteria and an enrichment of bacteria derived deleterious metabolites potentially associated with multiple types of diseases (such as ethyl glucuronide). The reduction of Ig heave chain variable domains may contribute to the increase of some Bacteroidetes species. Many bacteria ceramide lipids with a C17-sphingoid based were downregulated in COVID-19. In many cases, the gut phenome did not restore two months after symptom onset. Our study indicates widely disturbed gut molecular profiles which may play a role in the development of symptoms in COVID-19. Our findings also emphasis the need for ongoing investigation of the long-term gut molecular and microbial alterations during COVID-19 recovery process. Considering the gut ecosystem as a potential target could offer a valuable approach in managing the disease.
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http://dx.doi.org/10.1016/j.aca.2021.338881DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310733PMC
October 2021

New azaphilones from mangrove endophytic fungus SCNU-F0040.

Nat Prod Res 2021 Sep 9:1-9. Epub 2021 Sep 9.

School of Chemistry, Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, South China Normal University, Guangzhou China.

Two new sclerotioramines ( and ) and a new natural product of sclerotioramine analog (), together with seven known compounds have been isolated from the mangrove endophytic fungus SCNU-F0040. Their structures were identified based on the 1 D, 2 D NMR and HRESIM spectra. The absolute configurations of new compounds were deduced by specific rotation data and electronic circular dichroism spectra. All the isolated new compounds were tested on anti-diabetes activity by using a-glucosidase inhibition assay and anti-inflammatory activity by using cyclooxygenase inhibition assay, respectively. Compounds and have a-glucosidase inhibition activity with IC values of 102.3 and 217.5 μM. Compound shows a moderate cyclooxygenase-2 inhibitory activity with an IC value of 47.8 μM.
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http://dx.doi.org/10.1080/14786419.2021.1959580DOI Listing
September 2021

In Situ Defect Induction in Close-Packed Lattice Plane for the Efficient Zinc Ion Storage.

Small 2021 Sep 1:e2101944. Epub 2021 Sep 1.

School of Materials Science and Engineering, Central South University, Changsha, Hunan, 410083, P. R. China.

In situ electrochemical activation brings unexpected electrochemical performance improvements to electrode materials, but the mechanism behind it still needs further study. Herein, an electrochemically in situ defect induction in close-packed lattice plane of vanadium nitride oxide (VN O ) in aqueous zinc-ion battery is reported. It is verified by theoretical calculation and experiment that the original compact structure is not suitable for the insert of Zn ion, while a highly active one after the initial electrochemical activization accompanied by the in situ defect induction in close-packed lattice plane of VN O exhibits efficient zinc ion storage. As expected, activated VN O can achieve very high reversible capacity of 231.4 mA h g at 1 A g and cycle stability upto 6000 cycles at 10 A g with a capacity retention of 94.3%. This work proposes a new insight for understanding the electrochemically in situ transformation to obtain highly active cathode materials for the aqueous zinc-ion batteries.
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http://dx.doi.org/10.1002/smll.202101944DOI Listing
September 2021

PM exposure and maternal glucose metabolism in early pregnancy: Associations and potential mediation of 25-hydroxyvitamin D.

Ecotoxicol Environ Saf 2021 Aug 17;224:112645. Epub 2021 Aug 17.

Global Health Institute, Fudan University, Shanghai 200032, China; Key Laboratory of Public Health Safety, Ministry of Education, School of Public Health, Fudan University, Shanghai 200032, China. Electronic address:

Gestational diabetes mellitus (GDM) has become a new global epidemic with a rapidly increasing prevalence. Previous studies have suggested that air pollution is associated with GDM risk, but the results are inconsistent, and mechanistic studies are limited. Based on a hospital-based cohort, a total of 6374 participants were included in this study. Individual daily PM exposure at a 1-km resolution was predicted using a full-spatiotemporal-coverage model. The results of multiple linear regression showed that glycated hemoglobin (HbA1c) was significantly associated with PM both in the 1-month preconception and in the first trimester of pregnancy. Additionally, HbA1c decreased 0.437% (95% CI: -0.629, -0.244) as the serum 25-hydroxyvitamin D (25(OH)D) increased by one interquartile range (IQR) (9.2 ng/ml). An IQR increase in PM exposure was also negatively associated with serum 25(OH)D (estimated change% and 95% CI: -7.249 (-9.054, -5.408) in the 1-month preconception and - 13.069 (-15.111, -10.979) in the first trimester of pregnancy). Mediation analysis showed that serum 25(OH)D status mediated the association between HbA1c and PM exposure both in the preconception and in the first trimester (mediated percent: 2.00% and 4.05% (Sobel p<0.001), respectively). The result suggested a vicious cycle among PM exposure, lower serum VD status and a higher HbA1c. More studies are warranted since the protective effect of 25(OH)D against glucose disorders associated with air pollution in this study was limited.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112645DOI Listing
August 2021

Construction of Proton Transport Highways Induced by Polarity-Driving in Proton Exchange Membranes to Enhance the Performance of Fuel Cells.

ACS Appl Mater Interfaces 2021 Sep 19;13(34):40673-40684. Epub 2021 Aug 19.

Alan G. MacDiarmid Institute, College of Chemistry, Jilin University, Changchun 130012, P. R. China.

The approach to constructing proton transport channels via direct adjustments, including hydrophilia and analytical acid concentration in hydrophilic domains, has been proved to be circumscribed when encouraging the flatter hydrophilic-hydrophobic microphase separation structures and reducing conductivity activation energy. Here, we propose a constructive solution by regulating the polarity of hydrophobic domains, which indirectly varies the aggregation and connection of hydrophilic ion clusters during membrane formation, enabling orderly self-assembly and homogeneously distributed microphase structures. Accordingly, a series of comb-shaped polymers were synthesized with diversified optimization, and more uniformly distributed ion cluster lattices were subsequently observed using high-resolution transmission electron microscopy. Simultaneously, combining with density functional theory calculations, we analyzed the mechanism of membrane degradations caused by hydroxyl radical attacks. Experimental results demonstrated that, facilitated by proper molecule polarity, beneficial changes of bond dissociation energy could extend the membrane lifetime more than the protection from side chains near ether bonds, which were deemed to reduce the probability of attacks by the steric effect. With the optimal strategy chosen among various trials, the maximum power density of direct methanol fuel cell and H/air proton exchange membrane fuel cell was enhanced to 95 and 485 mW cm, respectively.
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http://dx.doi.org/10.1021/acsami.1c11715DOI Listing
September 2021

Isolation, Structural Characterization and Antidiabetic Activity of New Diketopiperazine Alkaloids from Mangrove Endophytic Fungus sp. 16-5c.

Mar Drugs 2021 Jul 20;19(7). Epub 2021 Jul 20.

Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, School of Chemistry, South China Normal University, Guangzhou 510006, China.

Six new DIKETOPIPERAZINE alkaloids aspergiamides A-F (-), together with ten known alkaloids (-), were isolated from the mangrove endophytic fungus sp. 16-5c. The structures of the new compounds were elucidated based on 1D/2D NMR spectroscopic and HR-ESIMS data analyses. The absolute configurations of aspergiamides A-F were established based on the experimental and calculated ECD data. All the compounds were evaluated for the antidiabetic activity against α-glucosidase and PTP1B enzyme. The bioassay results disclosed compounds and exhibited significant α-glucosidase inhibitory with IC values of 18.2 and 7.6 μM, respectively; compounds , , and exhibited moderate α-glucosidase inhibition with IC values ranging from 40.7 to 83.9 μM; while no compounds showed obvious PTP1B enzyme inhibition activity.
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http://dx.doi.org/10.3390/md19070402DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304462PMC
July 2021

First-principles investigations on a two-dimensional SN/black phosphorene van der Waals heterostructure: mechanical, carrier transport and thermoelectric anisotropy.

J Phys Condens Matter 2021 Aug 10;33(42). Epub 2021 Aug 10.

Hunan Key laboratory of Super Micro-structure and Ultrafast Process, School of Physics and Electronics, Central South University, Changsha 410083, People's Republic of China.

Isolated monolayer two-dimensional (2D) materials have attracted great attentions due to their unique optical, electrical, mechanical, thermoelectric properties and potential applications in nanoelectronic, optoelectronic and thermoelectric devices. However, it more and more difficult to find high performance and multifunctional monolayer 2D materials. The 2D van der Waals (vdW) heterostructure, which holds two different 2D materials together by vdW interactions, has opened up a new horizon in modulation of the energy band structure, the anisotropy of electrons and phonons, and the improvement of their thermoelectric properties for monolayer 2D materials. In this work, we theoretically investigated the anisotropy in the physical properties of 2D vdW heterostructure comprising of monolayer SNand black phosphorene (BP) using first-principles method. It is demonstrated that the ABstacking is the most stable dynamic and thermodynamics in the SN/BP heterostructure with vdW interaction between layers. The Young's modulus and Poisson's ratio of ABstacking along thedirection are 3 times of those along thedirection. Based on the Boltzmann transport theory within the relaxation time approximation, we demonstrated that ABstacking of SN/BP vdW heterostructure has significant anisotropy in the electron and phonon transport. Due to larger anharmonicity results in larger three-phonon scattering rates, the thermal conductivity of ABstacking of this heterostructure is half that of the pristine monolayer BP. We find the one with-type (-type) doping exhibits a peak figure of merit (ZT) value of 1.78 (0.52) at 300 K alongdirection, while those peak ZT value of 2.04 (0.69) alongdirection, exceeding the highest value of the monolayer BP doped with-type or-type doping. Our results would pave a way for applications to flexible and thermoelectric 2D materials.
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http://dx.doi.org/10.1088/1361-648X/ac1824DOI Listing
August 2021

COVID-19 negatively impacts on psychological and somatic status in frontline nurses.

J Affect Disord 2021 Nov 18;294:279-285. Epub 2021 Jul 18.

Department of Neurosurgery, The First Hospital of China Medical University, No. 155, Nanjing North Street, Heping District, Shenyang 110001, China. Electronic address:

Background: COVID-19 has become a public health emergency based on its clinical characteristics. Previous studies demonstrated that the onset of a sudden and immediately life-threatening illness could lead to extraordinary amounts of psychological pressure on nurses who play an important role in the illness. Whether COVID-19 pandemic has greater impacts on the psychological status and somatic symptoms from nurses who stand in the frontline of this crisis remain unclear.

Methods: We evaluated post-traumatic stress disorder (PTSD), anxiety and somatic symptoms in the frontline nurses (n = 438) who served in Wuhan, China, during COVID-19 crisis. Nurses who did not worked in the frontline of COVID-19 served as controls (n = 452). The investigation was processed by online questionnaires including: impact of event scale-revised (IES-R) , self-rating anxiety scale (SAS), and somatic symptoms.

Results: Prevalence of moderate and severe PTSD was significantly increased in the frontline nurses compared to non-frontline nurses. Prevalence of mild anxiety was significantly increased in frontline nurses compared to non-frontline nurses. There were more frontline nurses suffering from severe insomnia and losing weight compared to non-frontline nurses. Severity of PTSD (IES-R score), but not severity of anxiety (SAS score) was similarly positively correlated to incidence of insomnia and weight loss in both frontline and non-frontline nurses to a similar extent.

Limitations: The results only represented psychological statues and somatic symptom on one time point thus the development of psychological stress and somatic symptom during pandemic of COVID-19 in the frontline nurses were missing.

Conclusions: COVID-19 negatively impacted on psychological and somatic status in frontline nurses. PTSD may be the most reliability and validity criteria for evaluating psychological and somatic status for frontline nurses of COVID-19.
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http://dx.doi.org/10.1016/j.jad.2021.07.031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8286238PMC
November 2021

Identification of a circadian gene signature that predicts overall survival in lung adenocarcinoma.

PeerJ 2021 8;9:e11733. Epub 2021 Jul 8.

Department of Thoracic Surgery, The First Hospital of Jilin University, Changchun, Jilin Province, China.

Background: Lung adenocarcinoma (LUAD) is one of the most common subtypes of lung cancer which is the leading cause of death in cancer patients. Circadian clock disruption has been listed as a likely carcinogen. However, whether the expression of circadian genes affects overall survival (OS) in LUAD patients remains unknown. In this article, we identified a circadian gene signature to predict overall survival in LUAD.

Methods: RNA sequencing (HTSeq-FPKM) data and clinical characteristics were obtained for a cohort of LUAD patients from The Cancer Genome Atlas (TCGA). A multigene signature based on differentially expressed circadian clock-related genes was generated for the prediction of OS using Least Absolute Shrinkage and Selection Operator (LASSO)-penalized Cox regression analysis, and externally validated using the GSE72094 dataset from the GEO database.

Results: Five differentially expressed genes (DEGs) were identified to be significantly associated with OS using univariate Cox proportional regression analysis (P < 0.05). Patients classified as high risk based on these five DEGs had significantly lower OS than those classified as low risk in both the TGCA cohort and GSE72094 dataset (P < 0.001). Multivariate Cox regression analysis revealed that the five-gene-signature based risk score was an independent predictor of OS (hazard ratio > 1, P < 0.001). Receiver operating characteristic (ROC) curves confirmed its prognostic value. Gene set enrichment analysis (GSEA) showed that Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways related to cell proliferation, gene damage repair, proteasomes, and immune and autoimmune diseases were significantly enriched.

Conclusion: A novel circadian gene signature for OS in LUAD was found to be predictive in both the derivation and validation cohorts. Targeting circadian genes is a potential therapeutic option in LUAD.
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http://dx.doi.org/10.7717/peerj.11733DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8272922PMC
July 2021

confers enhanced the resistance to by increasing proanthocyanidin biosynthesis in cucumber.

Phytopathology 2021 Jul 20. Epub 2021 Jul 20.

College of Horticulture Science and Engineering, Shandong Agricultural UniversityTaian, China, 271018;

Root rot caused by Fusarium solani is one of the most common fungal diseases in cucumber (Cucumis sativus L.). Proanthocyanidins (PAs) are known to play important roles in inhibiting the growth of phytopathogens. In addition, CsMYB60 is a known positive regulator of flavonol and PA biosynthesis in cucumber. However, it remains unclear that whether PAs could inhibit the growth of F. solani and whether CsMYB60 serves as a target gene for enhancing resistance to phytopathogens in cucumber. In this study, we demonstrated that PAs (or their building block, catechin) could enhance the resistance of cucumber seedlings to F. solani both in vitro and in vivo. The addition of catechins, or crude leaf extracts treated with different concentrations of catechins in culture medium, could significantly inhibit the hyphal growth of F. solani. On the other hand, the cucumber seedlings treated with catechins showed higher resistance to F. solani than the seedlings in control group. Moreover, the transgenic cucumber seedlings overexpressing CsMYB60, with the observed accumulation of PAs, were more resistant to F. solani than the non-transgenic siblings. Therefore, our results suggest that PAs (or catechin) can serve as a biological control agent to protect cucumber plants from the infection of F. solani. More importantly, CsMYB60 holds great promise as a target gene to confer disease resistance during the molecular breeding in cucumber.
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http://dx.doi.org/10.1094/PHYTO-05-21-0223-RDOI Listing
July 2021

CsMYB60 directly and indirectly activates structural genes to promote the biosynthesis of flavonols and proanthocyanidins in cucumber.

Hortic Res 2020 Jul 1;7(1):103. Epub 2020 Jul 1.

State Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huang-Huai Region, Ministry of Agriculture, College of Horticultural Science and Engineering, Shandong Agricultural University, Tai'an, 271018, Shandong, China.

Flavonols and proanthocyanidins (PAs) are the main pigments in the black spines of cucumber (Cucumis sativus) fruit, and CsMYB60 is a key regulator of the biosynthesis of flavonols and PAs. However, in cucumber, the tissue distribution pattern of flavonols and PAs and the mechanism of their biosynthesis regulated by CsMYB60 remain unclear. In this study, we clarified the tissue-specific distribution of flavonoids and the unique transcriptional regulation of flavonoid biosynthesis in cucumber. CsMYB60 activated CsFLS and CsLAR by binding to their promoters and directly or indirectly promoted the expression of CsbHLH42, CsMYC1, CsWD40, and CsTATA-box binding protein, resulting in the formation of complexes of these four proteins to increase the expression of Cs4CL and interact with CsTATA-box binding protein to regulate the expression of CsCHS, thereby regulating the biosynthesis of flavonols and PAs in cucumber. Our data provide new insights into the molecular mechanism of flavonoid biosynthesis, which will facilitate molecular breeding to improve fruit quality in cucumber.
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http://dx.doi.org/10.1038/s41438-020-0327-zDOI Listing
July 2020

A survey of Prototheca bovis infection in dairy farms of the Hubei province, China.

J Vet Med Sci 2021 Aug 29;83(8):1248-1255. Epub 2021 Jun 29.

College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.

Bovine mastitis, caused by Prototheca bovis, has received much attention worldwide. To investigate the status of P. bovis infection in dairy farms of Hubei, we collected 1,158 milk samples and 90 environmental samples from 14 dairy farms of Hubei, China. The isolates were identified with traditional biological methods and molecular biological techniques, and their pathogenicity was tested through mice infection experiments. Isolates from 57 milk and 20 environmental samples were identified as P. bovis. The mice infection tests proved that the isolated P. bovis could cause mastitis in mice, manifesting as severe red swelling of the mammary glands. Histopathological analysis of tissue sections showed necrosis and nodules lesions formed in the infected mice mammary tissue, accompanied by macrophage and neutrophil infiltration. These results suggested the existence of pathogenic P. bovis in dairy farms of the Hubei province, China, with brewer's grains and fresh feces possibly playing important roles in the spread of this disease.
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http://dx.doi.org/10.1292/jvms.21-0068DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8437709PMC
August 2021

Investigation on the Efficiency of Chinese Herbal Injections combined with Concurrent Chemoradiotherapy for Treating Nasopharyngeal Carcinoma based on Multidimensional Bayesian Network Meta-analysis.

Front Pharmacol 2021 11;12:656724. Epub 2021 Jun 11.

Department of Clinical Chinese Pharmacy, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.

Given the wide utilization of Chinese herbal injections in the treatment of nasopharyngeal carcinoma (NPC), this network meta-analysis (NMA) was devised to compare the clinical efficacy and safety of different Chinese herbal injections combined with concurrent chemoradiotherapy (CCRT) against NPC. Randomized controlled trials (RCTs) were retrieved from seven electronic databases from the date of database establishment to October 5, 2020. Study selection and data extraction conformed to a priori criteria. Focusing on clinical effective rate, performance status, grade ≥3 oral mucositis, nausea and vomiting, leukopenia, and thrombopenia, this NMA was performed with Review Manager 5.3.5, Stata 13.1, WinBUGS 1.4.3, and R 4.0.3 software. Ten inventions from 37 RCTs involving 2,581 participants with NPC that evaluated the clinical effective rate, nausea and vomiting, leukopenia, thrombopenia, and grade ≥3 oral mucositis were included. Compared with CCRT alone, Elemene injection and Compound Kushen injection were associated with significantly improved clinical effective rates, and Elemene injection plus CCRT had the highest probability in terms of clinical effective rate (78.07%) compared with the other interventions. Shenqifuzheng injection, Xiaoaiping injection, and Shenmai injection ranked the best in terms of performance status (79.02%), nausea and vomiting (86.35%), and grade ≥3 oral mucositis (78.14%) when combined with CCRT. Kangai injection combined with CCRT ranked ahead of the other injections in terms of leukopenia (90.80%) and thrombopenia (91.04%), and had a better impact on improving performance status and reducing leukopenia, thrombopenia, grade ≥3 oral mucositis, and nausea and vomiting in the multidimensional cluster analysis. Current clinical evidence indicates that Elemene injection combined with CCRT has the best clinical effective rate and that Kangai injection might have a comprehensively better impact on improving performance status and reducing adverse reactions against NPC. Additionally, due to the limitations of this NMA, more multicenter, high-quality, and head-to-head RCTs are needed to properly support our findings.
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http://dx.doi.org/10.3389/fphar.2021.656724DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226160PMC
June 2021

Segregating domain-general from emotional context-specific inhibitory control systems - ventral striatum and orbitofrontal cortex serve as emotion-cognition integration hubs.

Neuroimage 2021 09 15;238:118269. Epub 2021 Jun 15.

The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Laboratory for Neuroinformation, Center for Information in Medicine, University of Electronic Science and Technology of China, Chengdu, China. Electronic address:

Inhibitory control hierarchically regulates cognitive and emotional systems in the service of adaptive goal-directed behavior across changing task demands and environments. While previous studies convergently determined the contribution of prefrontal-striatal systems to general inhibitory control, findings on the specific circuits that mediate emotional context-specific impact on inhibitory control remained inconclusive. Against this background we combined an evaluated emotional Go/No Go task with fMRI in a large cohort of subjects (N=250) to segregate brain systems and circuits that mediate domain-general from emotion-specific inhibitory control. Particularly during a positive emotional context, behavioral results showed a lower accuracy for No Go trials and a faster response time for Go trials. While the dorsal striatum and lateral frontal regions were involved in inhibitory control irrespective of emotional context, activity in the ventral striatum (VS) and medial orbitofrontal cortex (mOFC) varied as a function of emotional context. On the voxel-wise whole-brain network level, limbic and striatal systems generally exhibited highest changes in global brain connectivity during inhibitory control, while global brain connectivity of the left mOFC was less decreased during emotional contexts. Functional connectivity analyses moreover revealed that negative coupling between the VS with inferior frontal gyrus (IFG)/insula and mOFC varied as a function of emotional context. Together these findings indicate separable domain- general as well as emotional context-specific inhibitory brain systems which specifically encompass the VS and its connections with frontal regions.
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http://dx.doi.org/10.1016/j.neuroimage.2021.118269DOI Listing
September 2021

Asiatic Acid Induces Endoplasmic Reticulum Stress and Activates the Grp78/IRE1α/JNK and Calpain Pathways to Inhibit Tongue Cancer Growth.

Front Pharmacol 2021 26;12:690612. Epub 2021 May 26.

First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China.

Asiatic acid (AA) has been shown to induce apoptotic death in a range of cancers, but the mechanisms whereby it can inhibit tongue cancer growth have yet to be clarified. Herein, we explored the effects of AA on tongue cancer cells and found that it induced their apoptotic death and while additionally impairing xenograft tumor growth From a mechanistic perspective, AA treatment was associated with increases in levels of calcium and the calcium- dependent protease calpain, and it further induced endoplasmic reticulum (ER) stress and consequent Grp78-related IRE1α and JNK phosphorylation, ultimately driving caspase-3 activation and apoptotic death. Together, these results highlight AA as a promising tool for the therapeutic treatment of tongue cancer in clinical practice.
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http://dx.doi.org/10.3389/fphar.2021.690612DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187906PMC
May 2021

Research on the coordination of energy in China's economic growth.

PLoS One 2021 11;16(6):e0251824. Epub 2021 Jun 11.

State Power Investment Group Corporation, Beijing, China.

This study uses the improved Cobb-Douglas two-factor production function model to explore the potential relationship between economic growth and energy consumption through the multiple co-integration test on the panel data of China from 1985 to 2018. The results show that there is a positive long-term balance between energy consumption and economic growth: economic growth of 1%, total energy consumption growth of 1.53%, which means that economic growth needs higher energy support in the former short term. At the same time, the error correction term will converge energy consumption to a long-term equilibrium state with an adjustment intensity of 134.59%. From the results of variance decomposition, we can also see that as the number of periods increases, the part of real economic growth explained by energy consumption gradually increases.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0251824PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195397PMC
June 2021

Adaptive Neural Network Control for a Class of Fractional-Order Nonstrict-Feedback Nonlinear Systems With Full-State Constraints and Input Saturation.

IEEE Trans Neural Netw Learn Syst 2021 Jun 8;PP. Epub 2021 Jun 8.

This article addresses an adaptive neural network (NN) constraint control scheme for a class of fractional-order uncertain nonlinear nonstrict-feedback systems with full-state constraints and input saturation. The radial basis function (RBF) NNs are used to deal with the algebraic loop problem from the nonstrict-feedback formation based on the approximation structure. In order to overcome the problem of input saturation nonlinearity, a smooth nonaffine function is applied to approach the saturation function. To arrest the violation of full-state constraints, the barrier Lyapunov function (BLF) is introduced in each step of the backstepping procedure. By using the fractional-order Lyapunov stability theory and the given conditions, it proves that all the states remain in their constraint bounds, the tracking error converges to a bounded compact set containing the origin, and all signals in the closed-loop system are ensured to be bounded. Finally, the effectiveness of the proposed control scheme is verified by two simulation examples.
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http://dx.doi.org/10.1109/TNNLS.2021.3082984DOI Listing
June 2021

A systematic review of quantitative and qualitative literature on health professionals' experiences communicating with Chinese immigrants.

Int J Nurs Pract 2021 May 19:e12960. Epub 2021 May 19.

Charles Perkins Centre, Sydney Nursing School, University of Sydney, Sydney, New South Wales, Australia.

Aim: The aim of this study is to determine health professionals' experiences communicating with Chinese immigrants and identify potential education barriers.

Background: Health professionals caring for Chinese immigrants often encounter communication barriers, leading to uncertainty of quality of care.

Design: This study is a quantitative and qualitative systematic review.

Data Sources: MEDLINE, Scopus, CINAHL, PubMed and Google Scholar were searched, limited to 1980 to October 2020.

Review Methods: Articles were included if they reported results about health professional communication with Chinese patients. Quality was appraised using Consolidated Criteria for Reporting Qualitative Research guidelines and thematic synthesis conducted.

Results: Of 1363 articles, seven studies were included. These described provider-patient communication in primary care, oncology and palliative settings only. Three core themes were identified: (1) family-centred health communication where family controls provider-patient information exchange; (2) mismatch of provider-patient health beliefs and knowledge on diet, nutrition, traditional medicine, place for death and disease prevention and (3) mismatch of language and resources as skilled providers proficient in specific dialects are limited; communication resources are perceived as infrequently available and content is insufficient.

Conclusion: Studies describing health professionals' experiences communicating with Chinese immigrants are limited. Key barriers identified included cultural and language disparities and communication resources are inadequate to support health professionals' needs.
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http://dx.doi.org/10.1111/ijn.12960DOI Listing
May 2021

Environmental and Genetic Factors in the Pathogenesis of COPD in the Road-Working Population.

Dis Markers 2021 29;2021:9953234. Epub 2021 Apr 29.

State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.

Background: Chronic obstructive pulmonary disease (COPD) is a typical heterogeneous condition caused by environmental and genetic risk factors.

Objectives: We investigated extrinsic (environmental) and intrinsic (genetic) factors contributing to the development of COPD in a nonsmoker road-working population in Northeast China.

Method: The target population was divided into a COPD group and an exposed control group. Another healthy nonroad working nonsmoker control group was also included for environmental factor comparison. Peripheral blood was collected and analyzed using inductively coupled plasma mass spectrometry for inorganic elements of PM2.5, and microarray, rt-PCR, and Multiplex ELISA for genetic factors.

Results: Forty-three COPD road workers, thirty-nine non-COPD road workers, and 52 age and gender-matched healthy nonroad workers were enrolled. There were significantly higher levels in all 24 inorganic elements in the COPD group compared with the healthy control group except potassium and manganese, while the majority of inorganic elements were similar between the COPD group and the exposed control group except in aluminum and cobalt. There were 39 genes showing significant differences between the COPD group and the exposed control group. Collagen, type XV, alpha 1 (COL15A1), Meis homeobox 1 (MEIS1), carbonyl reductase 3 (CBR3), and amine oxidase, copper containing 3 (AOC3) were confirmed by rt-PCR to be differentially expressed. Their correlations with blood cytokines were also evaluated.

Conclusions: Aluminum might contribute to the development of COPD in the road-working population. CBR3 and AOC3 seem expressed in different patterns than previously reported, evidenced by their correlation with proinflammatory and anti-inflammatory cytokines.
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http://dx.doi.org/10.1155/2021/9953234DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105110PMC
April 2021

Improving the activity and stability of Bacillus clausii alkaline protease using directed evolution and molecular dynamics simulation.

Enzyme Microb Technol 2021 Jun 24;147:109787. Epub 2021 Mar 24.

Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, National Engineering Laboratory for Industrial Enzymes, The College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, PR China. Electronic address:

Detergent enzymes have been developed extensively as eco-friendly substitutes for the harmful chemicals in detergent. Among them, alkaline protease accounts for a large share of detergent enzyme sales. Thus, improving the specific activity of alkaline protease could play an important role in reducing the cost of detergent enzymes. In our study, alkaline protease from Bacillus clausii (PRO) was used to construct a mutant library through directed evolution using error-prone PCR, and a variant (G95P) with 9-fold enhancement in specific activity was obtained. After incubation at a pH of 11.0 for 70 h, G95P maintained 67 % of its maximal activity, which was 46 % more than wild-type PRO (WT), indicating that G95P has better alkaline stability than WT. The thermostability of G95P was also enhanced, as G95P achieved 17 % initial activity after incubation for 50 h at 60 °C, while WT lost its activity. The MD simulation results verified that variant G95P possessed improved stability of its Gly95-Gly100 loop region and Arg19-Asp265 salt bridge, leading to enhanced stability and catalytic efficiency. This work enhances the understanding of the structure-function relationship of PRO and provides an academic foundation for improving the enzymatic properties of PRO to satisfy industrial requirements using protein engineering.
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http://dx.doi.org/10.1016/j.enzmictec.2021.109787DOI Listing
June 2021

The transcriptional regulator Zur regulates the expression of ZnuABC and T6SS4 in response to stresses in Yersinia pseudotuberculosis.

Microbiol Res 2021 Aug 10;249:126787. Epub 2021 May 10.

State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, 712100, China. Electronic address:

Zinc homeostasis is crucial for the development and stress resistance of bacteria in the environment. Serial zinc sensing transcriptional regulators, zinc transporters and zinc binding proteins were found to maintain the zinc homeostasis in bacteria. Zur is a zinc uptake regulator that is widely distributed in species, and ZnuABC, as well as the Type VI Secretion System (T6SS4) function in zinc acquisition. Here, we report that the regulator Zur inhibits the expression of the ZnuABC which inhibition could be eliminated at low zinc level, and upregulates the T6SS4 operon in Yersinia pseudotuberculosis to facilitate Zn uptake and oxidative stress resistance. Zur regulates the expression of ZnuABC and T6SS4 by directly binding to their promoter regions. Zur senses the Zn concentration and represses ZnuABC in a Zn-containing environment. Zur works as an auxiliary regular activator of T6SS4, facilitating oxidative stress resistance. This study revealed the dual function of regulator Zur on ZnuABC and T6SS4, and enriched the knowledge of Zn homeostasis maintenance in Y. pseudotuberculosis.
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http://dx.doi.org/10.1016/j.micres.2021.126787DOI Listing
August 2021

Carrier type induces anammox biofilm structure and the nitrogen removal pathway: Demonstration in a full-scale partial nitritation/anammox process.

Bioresour Technol 2021 May 4;334:125249. Epub 2021 May 4.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China. Electronic address:

In this study, two typical carrier types, microporous and macroporous carriers, were collected from a full-scale partial nitritation/anammox reactor for analysis and comparison of the biofilm structure characteristics, performance and removal nitrogen pathway. For microporous carriers, a thicker biofilm (>5 mm) was obtained with higher biomass and abundance of anammox bacteria as well as a higher nitrogen removal efficiency due to the integration of denitrifying and anammox bacteria. In addition, higher microbial community stability can be expected under varying environmental conditions. In comparison, macroporous carrier biofilm exhibited a lower thickness (0.4-2.3 mm) and lower microbial richness, with a strong network correlation among genera. Analysis showed that the mainly positive correlation between anammox bacteria and ammonium oxidizing bacteria, enhancing coupling partial nitritation and anammox. These findings help further our understanding of the mechanisms of anammox biofilm nitrogen removal and provide a baseline for optimization of the design of carrier structures.
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http://dx.doi.org/10.1016/j.biortech.2021.125249DOI Listing
May 2021

Prenatal exposure to ambient fine particulate matter and early childhood neurodevelopment: A population-based birth cohort study.

Sci Total Environ 2021 Sep 24;785:147334. Epub 2021 Apr 24.

Key Lab of Health Technology Assessment, National Health Commission of the People's Republic of China (Fudan University), China; Key Laboratory of Public Health Safety, Ministry of Education, School of Public Health, Fudan University, Shanghai 200032, China. Electronic address:

Although previous studies have reported the adverse effect of air pollution exposure during pregnancy on neurodevelopment in children, epidemiological evidence is limited, and the results are inconsistent. This study aimed to explore the association between prenatal ambient fine particulate matter (PM) exposure and early childhood neurodevelopment in a large birth cohort study of 4009 maternal-child pairs. Prenatal daily PM exposure concentrations at 1 km spatial revolution were estimated using high-performance machine-learning models. Neurodevelopmental outcomes of children at ages 2, 6, 12, and 24 months were assessed using the Ages and Stages Questionnaire (ASQ). Distributed lag nonlinear models were used to identify critical windows of prenatal PM exposure. General linear mixed models with binomially distributed errors were used to estimate the effect of prenatal PM exposure on suspected developmental delay (SDD) in five developmental domains based on the longitudinal design. Prenatal PM exposure was significantly associated with decreased scores for all neurodevelopmental domains of children at ages 2, 6, and 24 months. Each 10-μg/m increase in PM exposure was significantly associated with increased risk of SDD for all subjects (RR: 1.52 95% CI: 1.19, 2.03), specifically, in problem-solving domain for girls (RR: 2.23, 95% CI: 1.22, 4.35). Prenatal PM exposure in weeks 18 to 34 was significantly associated with both ASQ scores and SDDs. Our study proposed that prenatal PM exposure affected early childhood neurodevelopment evaluated with the ASQ scale. PM exposure might increase the risk of SDD for boys and girls, specifically in the problem-solving domain for girls.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147334DOI Listing
September 2021

Co-Cropping Indian Mustard and Silage Maize for Phytoremediation of a Cadmium-Contaminated Acid Paddy Soil Amended with Peat.

Toxics 2021 Apr 21;9(5). Epub 2021 Apr 21.

The Institute of Agriculture, UWA School of Agriculture and Environment, The University of Western Australia, Perth, WA 6009, Australia.

Co-cropping is an eco-friendly strategy to improve the phytoremediation capacity of plants growing in soils contaminated with heavy metals such as cadmium (Cd). This study was conducted to investigate the effects of co-cropping Indian mustard () and silage maize () and applying peat on the phytoremediation of a Cd-contaminated acid paddy soil via characterizing plant growth and Cd uptake in pot experiments. There were six planting patterns (Control: no plants; MI-2 and MI-4: mono-cropping of Indian mustard at low and high densities, respectively; MS: mono-cropping of silage maize; CIS-2 and CIS-4: co-cropping of Indian mustard at low and high densities with silage maize, respectively) and two application rates of peat (NP: 0; WP: 30 g kg). When Indian mustard and silage maize were co-cropped, the shoot biomass of Indian mustard plants per pot was significantly ( < 0.05) lower than that obtained in the mono-cropping systems, with a substantial reduction (55-72%) in the same plant density group. The shoot biomass of silage maize plants in the mono-cropping systems did not differ significantly from that in the co-cropping systems regardless of the density of Indian mustard. The growth-promoting effect of the peat application was more pronounced in Indian mustard than silage maize. Under the low density of Indian mustard, the co-cropping systems significantly ( < 0.05) decreased Cd uptake by silage maize. Additionally, soil amendment with peat significantly ( < 0.05) increased shoot Cd removal rate and Cd translocation factor value in the co-cropping systems. Taken together, the results demonstrated that silage maize should be co-cropped with Indian mustard at an appropriate density in Cd-polluted soils to achieve simultaneous remediation of Cd-contaminated soils (via Indian mustard) and production of crops (here, silage maize). Peat application was shown to promote the removal of Cd from soil and translocation of Cd into shoots and could contribute to enhanced phytoremediation of Cd-contaminated acid paddy soil.
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http://dx.doi.org/10.3390/toxics9050091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143100PMC
April 2021

Surface-Preferred Crystal Plane for a Stable and Reversible Zinc Anode.

Adv Mater 2021 May 17;33(21):e2100187. Epub 2021 Apr 17.

School of Materials Science and Engineering, Central South University, Changsha, Hunan, 410083, China.

Aqueous zinc-ion batteries are largely restricted by the unsatisfactory performance of zinc (Zn) anodes, including their poor stability and irreversibility. In particular, the mechanism behind the electrochemical contrast caused by the surface crystal plane, which is a decisive factor of the electrochemical characteristics of the hostless Zn anode, is still relatively indistinct. Hence, new insight into a novel anode with a surface-preferred (002) crystal plane is provided. The interfacial reaction and morphology evolution are revealed by theoretical analysis and post-mortem/operando experimental techniques, indicating that Zn anodes with more exposed (002) basal planes exhibit free dendrites, no by-products, and weak hydrogen evolution, in sharp contrast to the (100) plane. These features benefit the Zn (002) anode by enabling a long cyclic life of more than 500 h and a high average coulombic efficiency of 97.71% for symmetric batteries, along with delivering long cycling stability and reversibility with life spans of over 2000 cycles for full batteries. This work provides new insights into the design of high-performance Zn anodes for large-scale energy storage and can potentially be applied to other metal anodes suffering from instability and irreversibility.
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http://dx.doi.org/10.1002/adma.202100187DOI Listing
May 2021

Simulation study on the migration of F in soil around Chagan Lake, China.

Environ Sci Pollut Res Int 2021 Sep 16;28(33):45155-45167. Epub 2021 Apr 16.

Key Laboratory of Groundwater Resources and Environment, Ministry of Education, Jilin University, Changchun, 130021, China.

Chagan Lake is located in the high-fluorine area of western Jilin, with high fluoride content in surface water, soil, and groundwater around it. Due to its unique topography and hydrogeological conditions, Chagan Lake collects surrounding water and is closely connected with groundwater. The complex surrounding water not only affects the groundwater quality through Chagan Lake, but also affects groundwater through the infiltration of vadose zone. In order to further study the characteristics of the migration of F in the soil around Chagan Lake along with water flow in the vadose zone and its impact on groundwater, soil column experiments were carried out using soil collected in the field, combined with HYDRUS-1D to simulate the migration characteristics of F. The model was verified by measured data, the sensitivity of each parameter was analyzed by the single-factor disturbance method, and the effect of F on groundwater was simulated and predicted. The results showed that (1) the soil column experiment was carried out using transport solutions of different pH value. The time required for F to penetrate the soil column under alkaline conditions was shorter, and the HYDRUS model used has a high degree of fitting. (2) The single-factor disturbance method is used to analyze the sensitivity of the parameters. The parameters that have a greater impact on the migration of fluoride ions in the soil are saturated hydraulic conductivity, adsorption coefficient, and soil bulk density. (3) In the prediction scenario, due to the adsorption and interception of the vadose zone, as the depth increases, the time it takes for F to accumulate is also longer. The groundwater around Chagan Lake is relatively shallow, and surface F in alkaline environment reaches the underground aquifer within 2 days. The F concentration in the groundwater reaches its maximum before the end of the irrigation period. In neutral environment, the F concentration in groundwater did not reach the maximum before the end of the irrigation period.
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http://dx.doi.org/10.1007/s11356-021-13635-wDOI Listing
September 2021

Identification and analysis of key genes associated with acute myocardial infarction by integrated bioinformatics methods.

Medicine (Baltimore) 2021 Apr;100(15):e25553

Department of Clinical Chinese Pharmacy, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.

Background: Acute myocardial infarction (AMI) is a common disease leading threat to human health around the world. Here we aimed to explore new biomarkers and potential therapeutic targets in AMI through adopting integrated bioinformatics tools.

Methods: The gene expression Omnibus (GEO) database was used to obtain genes data of AMI and no-AMI whole blood. Furthermore, differentially expressed genes (DEGs) were screened using the "Limma" package in R 3.6.1 software. Functional and pathway enrichment analyses of DEGs were performed via "Bioconductor" and "GOplot" package in R 3.6.1 software. In order to screen hub DEGs, the STRING version 11.0 database, Cytoscape and molecular complex detection (MCODE) were applied. Correlation among the hub DEGs was evaluated using Pearson's correlation analysis.

Results: By performing DEGs analysis, 289 upregulated and 62 downregulated DEGs were successfully identified from GSE66360, respectively. And they were mainly enriched in the terms of neutrophil activation, immune response, cytokine, nuclear factor kappa-B (NF-κB) signaling pathway, IL-17 signaling pathway, and tumor necrosis factor (TNF) signaling pathway. Based on the data of protein-protein interaction (PPI), the top 10 hub genes were ranked, including interleukin-8 (CXCL8), TNF, N-formyl peptide receptor 2 (FPR2), growth-regulated alpha protein (CXCL1), transcription factor AP-1 (JUN), interleukin-1 beta (IL1B), platelet basic protein (PPBP), matrix metalloproteinase-9 (MMP9), toll-like receptor 2 (TLR2), and high affinity immunoglobulin epsilon receptor subunit gamma (FCER1G). What's more, the results of correlation analysis demonstrated that there was positive correlation between the 10 hub DEGs.

Conclusion: Ten DEGs were identified as potential candidate diagnostic biomarkers for patients with AMI in present study. However, further experiments are needed to confirm the functional pathways and hub genes associated with AMI.
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http://dx.doi.org/10.1097/MD.0000000000025553DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052032PMC
April 2021

Evaluation of patient-reported outcome measures in intermittent self-catheterization users: A systematic review.

Arch Phys Med Rehabil 2021 Apr 8. Epub 2021 Apr 8.

School of Nursing, Tianjin Medical University, Observatory Road, Heping District, Tianjin 300070, People's Republic of China. Electronic address:

Objective: To identify patient-reported outcome measurements (PROMs) for intermittent self-catheterization (ISC) users, critically assess and summarize the quality of the measurement properties, and describe the application scenarios on each instrument.

Data Sources: PubMed, EMBASE, Medline, PsycINFO and relevant reference lists were systematically searched until December 2019 (updated May 2020).

Study Selection: Two reviewers independently identified original English language publications that evaluated the psychometric properties of specific PROMs used in ISC patients.

Data Extraction: The following data were obtained: author and publication year, content of domains/subscales, number of items, response options, constructs measured, language and information on measurement properties.

Data Synthesis: Eleven publications were deemed eligible, including 6 PROMs for measuring patients' ISC-related quality of life, self-confidence, satisfaction, difficulties, acceptance and adherence to treatment. The Intermittent Self-Catheterization Questionnaire provided the most detail, and the Intermittent Catheterization Acceptance Test could be evaluated on the most COSMIN properties.

Conclusion: Several tools are available for ISC users, but at present there is no comprehensive, concise and robust instrument with good psychometric properties. Further research on psychometric properties is needed to verify the remaining properties of existing scales and to develop novel tools for clinicians, researchers and patients.
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http://dx.doi.org/10.1016/j.apmr.2021.03.020DOI Listing
April 2021
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