Publications by authors named "Jia Lin"

530 Publications

Association of Pain With Atrial Fibrillation and Delirium After Cardiac Surgery: A DECADE Sub-Study.

J Cardiothorac Vasc Anesth 2021 May 15. Epub 2021 May 15.

Department of Outcomes Research, Cleveland Clinic, Cleveland, OH; Department of General Anesthesiology, Cleveland Clinic, Cleveland, OH. Electronic address:

Objective: The authors aimed to evaluate the effects of postoperative pain on the incidence of atrial fibrillation and delirium in patients having surgery with cardiopulmonary bypass (CPB).

Design: Post hoc analysis of the (An investigator-initiated, multicentre, double-blind trial (ClinicalTrials NCT02004613) (DECADE)), a randomized, placebo-controlled trial.

Setting: Tertiary, academic hospital.

Participants: Six hundred five adults from the DECADE enrolled at Cleveland Clinic Main Campus, who had had surgery with CPB.

Interventions: Dexmedetomidine versus placebo started before surgical incision and postoperatively was maintained until 24 hours.

Measurements: Primary outcomes were atrial fibrillation, diagnosed by clinicians in the intensive care unit (ICU), presence of delirium assessed with the Confusion Assessment Method for the ICU, data on pain scores, and opioid consumption, occurring between ICU admission and the earlier of postoperative day five or hospital discharge.

Results: Postoperative pain levels were similar among patients with or without atrial fibrillation. Two hundred six (34%) patients had atrial fibrillation and ninety-two (15%) had delirium before hospital discharge within the first five postoperative days. The risk of atrial fibrillation was not significantly different between groups (hazard ratio: 1.09; 97.5% confidence interval [CI]: 0.99, 1.20, p = 0.039); there were no associations between postoperative pain and the risk of postoperative delirium (hazard ratio: 0.96; 97.5% CI: 0.84-1.11; p = 0.57). Postoperative opioid consumption was neither significantly associated with postoperative atrial fibrillation nor delirium.

Conclusions: Atrial fibrillation and delirium was not associated with pain after cardiac surgery. Opioid use was not associated with atrial fibrillation and delirium. Because both atrial fibrillation and delirium have a multifactorial nature, further studies should be focused on other plausible mechanisms.
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http://dx.doi.org/10.1053/j.jvca.2021.05.013DOI Listing
May 2021

MXenes for Solar Cells.

Nanomicro Lett 2021 Feb 21;13(1):78. Epub 2021 Feb 21.

Key Laboratory of Special Function Materials and Structure Design of the Ministry of Education, and School of Physical Science and Technology, Lanzhou University, 222 South Tianshui Road, Lanzhou, 730000, People's Republic of China.

Application of two-dimensional MXene materials in photovoltaics has attracted increasing attention since the first report in 2018 due to their metallic electrical conductivity, high carrier mobility, excellent transparency, tunable work function and superior mechanical property. In this review, all developments and applications of the TiCT MXene (here, it is noteworthy that there are still no reports on other MXenes' application in photovoltaics by far) as additive, electrode and hole/electron transport layer in solar cells are detailedly summarized, and meanwhile, the problems existing in the related studies are also discussed. In view of these problems, some suggestions are given for pushing exploration of the MXenes' application in solar cells. It is believed that this review can provide a comprehensive and deep understanding into the research status and, moreover, helps widen a new situation for the study of MXenes in photovoltaics.
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http://dx.doi.org/10.1007/s40820-021-00604-8DOI Listing
February 2021

Improvisational Dance-Based Psychological Training of College Students' Dance Improvement.

Front Psychol 2021 26;12:663223. Epub 2021 May 26.

Faculty of the Professions, University of Adelaide, Adelaide, SA, Australia.

Dance creation involves complex psychological activities. Although previous studies have conducted extensive investigations on the psychological aspects of choreographers' creations, little is known regarding the psychological barrier of choreographers in terms of creativity. The study aims to explore the psychological barrier of innovation in dance choreography, which is a kind of situation between mental illness and mental problems. The research shows that improvisational dance is a free dance with the human body as a material carrier, and it is a dance form that the dancer's thinking is transformed into dance action to express their inner thoughts. The improvement of the potential creative thinking of dance creators through the improvisational dance movement and structure are explored. Meanwhile, the theoretical ideas of psychologists have been applied to the cultivation and improvement of improvisational dance creativity, which has made full certification and supplement. Moreover, through psychological experiments, it is proved that improvisational dance is an important way to release and develop creativity. Based on the theory of psychology, feasible suggestions are given to improve students' creative ability in the process of improvisational dance teaching.
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http://dx.doi.org/10.3389/fpsyg.2021.663223DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189290PMC
May 2021

Decreased Transfusion Requirements with Use of Acute Normovolemic Hemodilution in Open Aortic Aneurysm Repair.

J Vasc Surg 2021 May 31. Epub 2021 May 31.

Department of Vascular Surgery, Cleveland Clinic, Cleveland, OH. Electronic address:

Objectives: Acute normovolemic hemodilution (ANH) is an operative blood conservation technique involving the removal and storage of patient blood after induction of anesthesia, while maintaining normovolemia with crystalloid and/or colloid replacement. Developed and used predominately in cardiac surgery, ANH has been applied to the vascular surgery population but data regarding impact on transfusion requirements in this population is limited. The objective of this study is to compare transfusion requirements and coagulopathy in patients undergoing open abdominal aortic aneurysm repair (oAAA) employing ANH to those receiving only product replacement as clinically indicated.

Methods: This is a retrospective review of patients undergoing elective oAAA at a quaternary aortic referral center from 2017-2019. Those eligible for ANH, no active cardiac ischemia, no valvular disease, normal LV/RV function, CKD < stage 3, HCT > 38%, normal coagulation profile, were included in the study. Patient demographics and characteristics were collected as were operative variables including extent of aneurysm, clamp site, visceral/renal ischemia time, operative time, and transfusion requirements. Post-operative morbidity, mortality, and length of stay were analyzed. Patients with and without ANH were matched and compared. Continuous measures were analyzed with Wilcoxon rank sum tests and t-tests.

Results: Over the study period, 209 oAAA were performed, 76 patients met inclusion criteria; 27 patients underwent ANH while 49 did not. Patients with ANH had less PRBC transfusion intraoperative (Median [25,75]: 0 [0,1] vs. 1[0,2] units, p=0.02), at 24 hours(0[0,1] vs. 1[0,2] units, p=0.008), at 48 hours (0[0,1] vs. 1[0,2], p=0.007) and throughout the admission (0[0,1] vs. 2[0,2], p=0.011). There was no difference in intraoperative platelet or cryoprecipitate transfusions. At 48-hours, ANH had significantly higher platelet counts (142 ±35.8 vs. 124±37.6 x10(3)/mcL, p=0.044), lower PTT, and lower INR. There was no difference in MI, RTOR, or mortality (1 death overall). ANH patients had shorter LOS (7.0±2.7 vs 8.8±4.8 days, p=0.041).

Conclusions: ANH during oAAA results in less intraoperative and post-operative PRBC with improved coagulation parameters and a shorter hospital LOS.
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http://dx.doi.org/10.1016/j.jvs.2021.05.030DOI Listing
May 2021

Host-Seeking Behavior of Aphidius gifuensis (Hymenoptera: Braconidae) Modulated by Chemical Cues Within a Tritrophic Context.

J Insect Sci 2021 May;21(3)

Biological Control Research Institute, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.

Aphidius gifuensis Ashmaed is a generalist endoparasitoid that parasitizes a variety of aphid species. In China, it is widely used as a biological control agent to protect vegetables and tobaccos in open fields; control efficiency is largely dependent on its host-seeking ability. In this study, a six-choice olfactometer was used to investigate the olfactory responses of A. gifuensis to tobacco plants that had suffered damage (either varying degrees of mechanical damage or from aphid-feeding at different time intervals) and tobacco volatiles with different dosages. Furthermore, the regularity of A. gifuensis females' response toward an aphid/tobacco complex was monitored using a Y-tube olfactometer. Our findings suggest that tobacco plants are significantly attractive to A. gifuensis after they have been punctured with 50 holes, or housed with Myzus persicae (Sulzer) at a density of 400 aphids, except at an infestation time of 12 h. Moreover, aphid density had a more significant effect on the response than the time interval since aphid application. Aphidius gifuensis was found to be active during the daytime and preferred to search for their aphid hosts at 14:00 h. Five EAG-active tobacco volatiles (trans-2-hexenal, methyl salicylate, benzaldehyde, cis-3-hexen-1-ol, and 1-hexanal) were found to significantly attract A. gifuensis females at different concentration ranges. The practical implications of these results are discussed in the framework of the sustainable biological control of pest aphids in agricultural production systems.
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http://dx.doi.org/10.1093/jisesa/ieab036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161523PMC
May 2021

Multimodality Treatment Improves Locoregional Control, Progression-Free and Overall Survival in Patients with Anaplastic Thyroid Cancer: A Retrospective Cohort Study Comparing Oncological Outcomes and Morbidity between Multimodality Treatment and Limited Treatment.

Ann Surg Oncol 2021 May 25. Epub 2021 May 25.

University of Sydney Endocrine Surgical Unit, Royal North Shore Hospital, Northern Sydney Local Health District, St Leonards, Australia.

Background: Patients with anaplastic thyroid cancer (ATC) have poor overall survival, and the optimal management approach remains unclear. The aim of this study is to evaluate our experience with multimodality (MMT) versus limited treatment (LT) for ATC.

Patients And Methods: A cohort study of patients with ATC managed in a tertiary referral center was undertaken. The outcomes of MMT were compared with those of LT. The primary outcome measures were locoregional control and progression-free and overall survival. Secondary outcome measures were treatment-related complications and factors associated with improved survival.

Results: In total, 59 patients (35 females) with a median age of 73 years (range 39-99 years) and ATC stage IVA (n = 2), IVB (n = 28), or IVC (n = 29) were included. LT was utilized in 25 patients (42%), and 34 cases had MMT. MMT patients had a longer time of locoregional control (18.5 versus 1.9 months; p < 0.001), progression-free survival (3.5 versus 1.2 months; p < 0.001), and overall survival (6.9 versus 2.0 months; p < 0.001) when compared with LT. For patients with stage IVC ATC, locoregional control (p = 0.03), progression-free survival (p < 0.001), and overall survival (p < 0.001) were superior in the MMT cohort compared with LT. MMT had more treatment-related complications than LT (p < 0.001). An Eastern Cooperative Oncology Group performance status < 2 (HR 0.30; p = 0.001) and MMT (HR 0.35; p = 0.008) were associated with improved overall survival.

Conclusion: MMT is likely to improve locoregional control, progression-free survival, and overall survival in selected ATC patients including stage IVC tumors but comes with a greater complication risk.
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http://dx.doi.org/10.1245/s10434-021-10146-3DOI Listing
May 2021

Stent insertion for inoperable hilar cholangiocarcinoma: Comparison of radioactive and normal stenting.

Medicine (Baltimore) 2021 May;100(21):e26192

Department of Interventional Radiology, Jiangyin People's Hospital, Jiangyin, China.

Abstract: To assess effectiveness and safety associated with radioactive stenting for hilar cholangiocarcinoma (HCCA) patients.This single-center retrospective study compared baseline and treatment data of recruited consecutive patients with HCCA underwent either normal or radioactive stenting between January 2016 and December 2019. Clinical success was defined by total bilirubin (TBIL) levels falling below 70% of the preoperative baseline within 2 weeks post stent insertion.Sixty-five patients with inoperable HCCA underwent normal (n = 35) or radioactive (n = 30) stenting at our center. Technical success of both types of the normal and radioactive stent insertion was 100%. Each patient received 1 stent. In the radioactive stent group, each patient received 1 radioactive seed strand (RSS), containing 10 to 12 radioactive seeds. Clinical success rates were 86.8% and 100% in normal and radioactive groups, respectively (P = .495). We observed stent dysfunction in 9 patients (normal group) and 7 patients (radioactive group) (P = .824). Median duration of stent patency was 165 days (normal group) and 226 days (radioactive group) (P < .001). During follow-up, all patients died from tumor progression, with respective median survival of 198 days (normal group) and 256 days (radioactive group) (P < .001). Seven and 5 patients in the normal and radioactive groups suffered from stent-related complications (P = .730).Radioactive stenting is effective and safe for inoperable HCCA patient and may prolong stent patency and survival.
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http://dx.doi.org/10.1097/MD.0000000000026192DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154471PMC
May 2021

Chinese Aesthetic Mask: Three Forehead and Five Eyes-Holistic Processing and Facial Attractiveness.

Authors:
Jia Lin Guomei Zhou

Perception 2021 Jun 18;50(6):540-554. Epub 2021 May 18.

26469Sun Yat-Sen University, China.

Human face processing has been attributed to holistic processing. Here, we ask whether humans are sensitive to configural information when perceiving facial attractiveness. By referring to a traditional Chinese aesthetic theory-Three Forehead and Five Eyes-we generated a series of faces that differed in spacing between facial features. We adopted a two-alternative forced-choice task in Experiment 1 and a rating task in Experiment 2 to assess attractiveness. Both tasks showed a consistent result: The faces which fit the Chinese aesthetic theory were chosen or rated as most attractive. This effect of configural information on facial attractiveness was larger for faces with highly attractive features than for faces with low attractive features. These findings provide experimental evidence for the traditional Chinese aesthetic theory. This issue can be further explored from the perspective of culture in the future.
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http://dx.doi.org/10.1177/03010066211015542DOI Listing
June 2021

The atypical antipsychotic risperidone targets hypothalamic melanocortin 4 receptors to cause weight gain.

J Exp Med 2021 Jul 12;218(7). Epub 2021 May 12.

The Hypothalamic Research Center, Department of Internal Medicine, The University of Texas Southwestern Medical Center, Dallas, TX.

Atypical antipsychotics such as risperidone cause drug-induced metabolic syndrome. However, the underlying mechanisms remain largely unknown. Here, we report a new mouse model that reliably reproduces risperidone-induced weight gain, adiposity, and glucose intolerance. We found that risperidone treatment acutely altered energy balance in C57BL/6 mice and that hyperphagia accounted for most of the weight gain. Transcriptomic analyses in the hypothalamus of risperidone-fed mice revealed that risperidone treatment reduced the expression of Mc4r. Furthermore, Mc4r in Sim1 neurons was necessary for risperidone-induced hyperphagia and weight gain. Moreover, we found that the same pathway underlies the obesogenic effect of olanzapine-another commonly prescribed antipsychotic drug. Remarkably, whole-cell patch-clamp recording demonstrated that risperidone acutely inhibited the activity of hypothalamic Mc4r neurons via the opening of a postsynaptic potassium conductance. Finally, we showed that treatment with setmelanotide, an MC4R-specific agonist, mitigated hyperphagia and obesity in both risperidone- and olanzapine-fed mice.
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http://dx.doi.org/10.1084/jem.20202484DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8126977PMC
July 2021

AQP2 as a target of lycopene protects against atrazine-induced renal ionic homeostasis disturbance.

Food Funct 2021 Jun;12(11):4855-4863

College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, P. R. China. and Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Northeast Agricultural University, Harbin, 150030, P. R. China and Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Northeast Agricultural University, Harbin, 150030, P. R. China.

Atrazine (ATR), a ubiquitous environmental contaminant in water and soil, causes environmental nephrosis. To reveal the toxic effect of ATR on the kidney and the potential chemical nephroprotective effect of lycopene (LYC), Kun-Ming mice of specific pathogen-free (SPF) grade were treated with LYC (5 mg kg-1) and/or ATR (50 mg kg-1 or 200 mg kg-1) for 21 days. The degree of renal injury was evaluated by measuring the ion concentration, ATPase activities and the mRNA expressions/levels of associated ATPase subunits. In addition, the expression of renal aquaporins (AQPs) was analyzed. The results showed that the renal tubular epithelial cells of ATR-exposed mice were swollen, the glomeruli were significantly atrophied, and the ion concentrations were obviously changed. The activity of Na+-K+-ATPase and the transcription of its subunits were downregulated. The activity of Ca2+-Mg2+-ATPase and the transcription of its subunits were upregulated. The expression of AQPs, especially the critical AQP2, was affected. Notably, ATR-induced nephrotoxicity was significantly improved by LYC supplementation. Therefore, LYC could protect the kidney against ATR-induced nephrotoxicity via maintaining ionic homeostasis, reversing the changes in ATPase activity and controlling the expression of AQPs on the cell membrane. These results suggested that AQP2 was a target of LYC and protected against ATR-induced renal ionic homeostasis disturbance.
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http://dx.doi.org/10.1039/d0fo03214jDOI Listing
June 2021

Enhanced adsorption properties of organic ZnCr-LDH synthesized by soft template method for anionic dyes.

Environ Sci Pollut Res Int 2021 Apr 27. Epub 2021 Apr 27.

School of Chemistry and Chemical Engineering, Henan Key Laboratory of Coal Green Conversion, Henan Polytechnic University, Jiaozuo, 454000, China.

Organic ZnCr-LDH (ST-LDH) was synthesized by a facile one-step hydrothermal technique using methyl orange (MO) as a soft template agent, which can efficiently remove methyl orange (MO), Congo red (CR), and orange II (OII) from aqueous solution. The microstructure of ST-LDH by modifying changed obviously, from the cellular structure to the stacking structure formed by the face-face contact of hydrotalcite nanosheets, which resulted in much more exchangeable nitrate ions to remain in the interlayer space. The pre-insertion of benzene sulfonate as a pillar expanded the interlayer gallery, which facilitated the pollutant anions (MO, CR, and OII) into the interlayer of LDH in the subsequent adsorption process. The maximum adsorption capacity of ST-LDH for MO, CR, and OII was 4200.8 mg/g, 1252.0 mg/g, and 1670.6 mg/g, respectively, which is approximately 1.86 times, 1.8 times, and 2.32 times that of the pristine NO-LDH, respectively. The removal mechanism of anionic dyes was determined as anion exchange between NO anions and dye molecules. The adsorption behavior for MO and OII is multilayer adsorption, while the adsorption behavior for CR is monolayer adsorption. The adsorption process mainly was controlled by the chemical bonding between the dye molecules and adsorbent active sites. The LDH can be effectively regenerated by photocatalysis after MO adsorption. The ST-LDH has a great potential to be used as a high-efficient adsorbent to remove anionic dyes from aqueous solution. The schematic illustration of the synthetic process of soft template agent modified and unmodified hydrotalcites by one-pot hydrothermal method and the adsorption process of MO by ST-LDH were shown in Fig. 12. Modified hydrotalcite (ST-LDH) was prepared using methyl orange (MO) as a soft template agent. Compared with unmodified hydrotalcite (NO-LDH), the insertion of benzene sulfonate anions into the hydrotalcite layer resulted in the increase of the interlayer spacing from 8.269 to 8.654Å. The LDH host structure pre-intercalated by benzene sulfonate anions evolved into pillared materials in interlayer; benzene sulfonate anions as a column expanded the interlayer spacing of (003) base plane, which facilitated the pollutant anions (MO, CR, and OII) into the interlayer of ST-LDH and exchanged with NO anion in the subsequent adsorption process. It can be inferred that in the process of modification hydrotalcite by benzene sulfonate, a small amount of benzene sulfonate anions pre-inserted into the gallery of hydrotalcite with a monolayer model in the process of hydrotalcite modification, and its inclination angle is calculated to be about 29.1°. After ST-LDH sample adsorbed the MO molecules, dye molecules intercalated into the LDH host, and successful exchange with NO anions, the d value increased to 24.78 Å. A large amount of MO anions were intercalated into the gallery of ST-LDH with a bilayer model according to the Freundlich isotherm model, and the tilting angle increases to 53.6°. The adsorption capacity of MO by ST-LDH was significantly enhanced to 4200.8 mg/g, which was much higher than that of NO-LDH (2252.8 mg/g). Schematic illustration of the synthetic process of LDH materials and adsorption process of MO by ST-LDH.
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http://dx.doi.org/10.1007/s11356-021-14035-wDOI Listing
April 2021

Two-Dimensional Palladium-Copper Alloy Nanodendrites for Highly Stable and Selective Electrochemical Formate Production.

Nano Lett 2021 May 21;21(9):4092-4098. Epub 2021 Apr 21.

Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou 215123, China.

Pd is the only metal that can catalyze electrochemical CO reduction to formate at close-to-zero overpotential. It is unfortunately subjected to severe poisoning by trace CO as the side product and suffers from deteriorating stability and selectivity with increasing overpotential. Here, we demonstrate that alloying Pd with Cu in the form of two-dimensional nanodendrites could enable highly stable and selective formate production. Such unique bimetallic nanostructures are formed as a result of the rapid in-plane growth and suppressed out-of-plane growth by carefully controlling a set of experimental parameters. Thanks to the combined electronic effect and nanostructuring effect, our alloy product catalyzes CO reduction to formate with remarkable stability and selectivity under the working potential as cathodic as -0.4 V. Our results are rationalized by computational simulations, evidencing that Cu atoms weaken the *CO adsorption and stabilize the *OCHO adsorption on neighboring Pd atoms.
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http://dx.doi.org/10.1021/acs.nanolett.1c01113DOI Listing
May 2021

QTL mapping of the genetic basis of stem diameter in soybean.

Planta 2021 Apr 19;253(5):109. Epub 2021 Apr 19.

Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Zhengzhou, 450002, China.

Main Conclusion: QTL mapping of stem diameter was carried out in three RIL populations using a high-density genetic map, and candidate genes related to stem diameter were predicted. Stem diameter is an important agronomic trait affecting soybean lodging and productivity. However, this trait is underexploited, and the underlying genetic mechanism in soybean remains unclear. In this study, three recombinant inbred line (RIL) populations, including 156 F lines from Nannong 94-156 × Bogao (N × B), 127 F lines from Dongnong 50 × Williams 82 (D × W), and 146 F lines from Suinong 14 × Enrei (S × E), were used to identify QTLs for soybean stem diameter across multiple environments. Phenotype analysis revealed that stem diameter exhibited strong positive correlations with plant height and 100-seed weight, two of the most important yield components. A total of 12 QTLs for stem diameter were identified on eight chromosomes across three RIL populations and five environments. The most influential QTL that was stably identified across all the populations and environments, q11, explained 12.58-26.63% of the phenotypic variation. Detection of several environment-specific QTLs, including q14, q16, and q20, suggests that environments may also have important effects in shaping the natural variation in soybean stem diameter. Furthermore, we predicted candidate genes underlying the QTLs and found that several promising candidate genes may be responsible for the variation in stem diameter in soybean. Overall, the markers/genes linked closely or underlying the major QTLs may be used for marker-assisted selection of soybean varieties to enhance lodging resistance and even yield. Our results lay the foundation for the fine mapping of stem development-related genes to reveal the molecular mechanisms.
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http://dx.doi.org/10.1007/s00425-021-03628-xDOI Listing
April 2021

[Clinical Blood Utilization of Patients at Different Ages for a Hospital in Sichuan from 2017 to 2018].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Apr;29(2):620-628

Department of Blood Transfusion, Sichuan Academy of Medical Sciences, Sichuan Provincial People's Hospital, Chengdu 610072, Sichuan Province,

Objective: To analyze the use of blood products in patients at different ages.

Methods: The clinical datas of the 10 784 patients transfused in Sichuan provincial people's hospital at 2017-2018 were retrospectively analyzed, and the basic condition of clinical blood using was statistically described. The patients were divided into the groups according to age and disease, then the use of various blood products in the patients with different diseases in different age groups was analyzed.

Results: The age of blood transfusion patients was mainly 40-80 years old, and the most common disease was tumor(about 28%). The average annual transfusion volumes of red blood cells(RBC) were 24 936.5 U, of platelets(PLT) were 3 795 therapeutic doses of plasma were 2 455 500 ml, of cryoprecipitate were 3 461.5 U in our hospital. Most patients with hematologic malignancies and liver cirrhosis were transfused with two or more blood productions. For the patients with hematologic malignancies, the irradiated RBC (76.4%), PLT (67.8%), and suspended RBC (59.9%) were commonly used. And for liver cirrhosis patients, the suspended RBC (64.2%) and fresh frozen plasma(FFP) (59.4%) were commonly used. For the patients with trauma and chronic kidney disease(CKD), the suspended RBC (95.7% and 91.5%, respectively) was commonly used. In hematologic malignancies patients, the transfusion volume of irradiated RBC, PLT and FFP in the patients aged ≥60 years old was lower than that in patients aged<60 years old (P<0.05); in trauma patients, the transfusion volume of suspended RBC in the patients aged ≥60 years old was lower than that in patients aged<60 years old (P<0.05). In hematologic malignancies, trauma and liver cirrhosis patients, the proportion of PLT and/or plasma transfusion in the patients aged ≥60 years old was lower than that in patients aged<60 years old (P<0.05), and the elderly patients were more likely to receive RBC transfusion only.

Conclusion: There is a difference in the distribution of blood product between the patients aged<60 years old and ≥60 years old in the same disease, and it is more likely that the elderly patients (aged ≥60 years old) receive RBC transfusion only, and correction of hypoxia is a major clinical consideration, so blood using plans should be made according to the patient population, moreover, the different transfusion strategies should be developed for different population to maximize the efficiency of blood using.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2021.02.050DOI Listing
April 2021

Gallbladder gangrene after percutaneous vertebroplasty, an uncommon presentation of vascular complication: a case report and analysis of the causes.

J Int Med Res 2021 Mar;49(3):3000605211001720

Department of Hepatobiliary, Pancreatic, and Splenic Surgery, 117872Hebei General Hospital, Shijiazhuang, China.

We present a case of an 81-year-old man with gallbladder gangrene after percutaneous vertebroplasty (PV) that was successfully treated via laparoscopic cholecystectomy (LC). The patient underwent multilevel, thoracic PV for painful osteoporotic compression fractures. PV performed at the T6 level was complicated by severe abdominal pain owing to direct embolization of the right T6 segmental artery with penetration of bone cement into the radicular artery beneath the pedicle. Cement leakage, especially arterial embolization of cement into the general circulation, is a known potential complication following PV. Serious complications related to PV augmentation procedures, such as vertebroplasty and kyphoplasty, are rare and most often result from local cement leakage or venous embolization. Combined with this case report, we reviewed the literature regarding the unusual occurrence of direct arterial cement embolization during PV and analyzed the causes to alert clinicians to this potentially rare vascular complication.
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http://dx.doi.org/10.1177/03000605211001720DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020101PMC
March 2021

Mortality predictors among patients with HIV-associated pulmonary tuberculosis in Northeast China: A retrospective cohort analysis.

J Med Virol 2021 Mar 31. Epub 2021 Mar 31.

Department of Respiratory and Infectious Diseases, Beijing You An Hospital, Capital Medical University, Beijing Institute of Hepatology, Beijing, China.

The coexistence of pulmonary tuberculosis (PTB) and human immunodeficiency virus (HIV) infection leads to high morbidity and mortality in these populations. Although antiretroviral therapy (ART) has decreased TB incidence in HIV-infected patients, this coexistence still prevails in China. Patients with HIV-PTB admitted to Beijing You An Hospital from 2014 to 2018 were retrospectively enrolled, and information on demographics, clinical characteristics, and laboratory findings were extracted from medical records. Predictors of death, including age (adjusted hazard ratio [AHR]: 1.03; 95% confidence interval [CI]: 1.00-1.05), tobacco use (AHR: 2.76; 95% CI: 1.54-4.94), history of tuberculosis (AHR: 3.53; 95% CI: 1.82-6.85), not being on ART (AHR: 2.94; 95% CI: 1.31-6.63), extrapulmonary tuberculosis (AHR: 2.391; 95% CI: 1.37-4.18), sputum smear positivity (AHR: 2.84; 95% CI: 1.61-4.99), CD4 T cell count ≤ 50 cells/µl (AHR: 3.45; 95% CI: 1.95-6.10), and initiating ART ≥ 8 weeks after the initiation of antituberculous therapy (odds ratio: 3.30; 95% CI: 1.09-10.04). By contrast, there were no deaths among the six patients who began ART within 8 weeks after the initiation of antituberculous therapy. Age, tobacco use, not being on ART, extrapulmonary tuberculosis, sputum smear positivity, and CD4 T cell count ≤50 cells/µl predict those patients at high risk of death among HIV-infected patients with PTB, and the time of initiating ART after the initiation of antituberculous therapy is also important for prognosis.
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http://dx.doi.org/10.1002/jmv.26977DOI Listing
March 2021

Smoking and nasopharyngeal cancer: individual data meta-analysis of six prospective studies on 334 935 men.

Int J Epidemiol 2021 Mar 31. Epub 2021 Mar 31.

School of Public Health, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong.

Background: The role of smoking in nasopharyngeal carcinoma (NPC) remains uncertain, especially in endemic regions. We conducted an individual participant data (IPD) meta-analysis of prospective cohort studies to investigate the associations between smoking exposure and risk of NPC.

Methods: We obtained individual participant data of 334 935 male participants from six eligible population-based cohorts in NPC-endemic regions, including two each in Guangzhou and Taiwan, and one each in Hong Kong and Singapore. We used one- and two-stage approaches IPD meta-analysis and Cox proportional hazard models to calculate hazard ratios (HRs) and 95% confidence intervals (CIs) of NPC for smoking exposure adjusting for age and drinking status.

Results: During 2 961 315 person-years of follow-up, 399 NPC evens were ascertained. Risks of NPC were higher in ever versus never smokers (HRone-stage = 1.32, 95% CI = 1.07-1.63, P = 0.0088; HRtwo-stage = 1.27, 1.01-1.60, 0.04). These positive associations appeared to be stronger in ever smokers who consumed 16+ cigarettes/day (HRone-stage = 1.67, 95% CI = 1.29-2.16, P = 0.0001), and in those who started smoking at age younger than 16 (2.16, 1.33-3.50, 0.0103), with dose-response relationships (P-values for trend = 0.0028 and 0.0103, respectively). Quitting (versus daily smoking) showed a small reduced risk (stopped for 5+ years: HRone-stage = 0.91, 95% CI = 0.60-1.39, P = 0.66; for former smokers: HRtwo-stage = 0.84, 0.61-1.14, 0.26).

Conclusions: This first IPD meta-analysis from six prospective cohorts in endemic regions has provided robust observational evidence that smoking increased NPC risk in men. NPC should be added to the 12-16 cancer sites known to be tobacco-related cancers. Strong tobacco control policies, preventing young individuals from smoking, would reduce NPC risk in endemic regions.
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http://dx.doi.org/10.1093/ije/dyab060DOI Listing
March 2021

There is no dose-response relationship between allogeneic blood transfusion and healthcare-associated infection: a retrospective cohort study.

Antimicrob Resist Infect Control 2021 03 29;10(1):62. Epub 2021 Mar 29.

Healthcare-Associated Infection Management Office, Sichuan Academy of Medical Sciences and Sichuan People's Hospital, Chengdu, 610072, Sichuan, People's Republic of China.

Background: The association between allogeneic blood transfusion and healthcare-associated infection (HAI) is considered dose-dependent. However, this association may be confounded by transfusion duration, as prolonged hospitalization stay increases the risk of HAI. Also, it is not clear whether specific blood products have different dose-response risks.

Methods: In this retrospective cohort study, a logistic regression was used to identify confounding factors, and the association between specific blood products and HAI were analyzed. Then Cox regression and restricted cubic spline regression was used to visualize the hazard of HAI per transfusion product.

Results: Of 215,338 inpatients observed, 4.16% were transfused with a single component blood product. With regard to these transfused patients, 480 patients (5.36%) developed a HAI during their hospitalization stay. Logistic regression showed that red blood cells (RBCs) transfusion, platelets transfusion and fresh-frozen plasmas (FFPs) transfusion were risk factors for HAI [odds ratio (OR) 1.893, 95% confidence interval (CI) 1.656-2.163; OR 8.903, 95% CI 6.646-11.926 and OR 1.494, 95% CI 1.146-1.949, respectively]. However, restricted cubic spline regression analysis showed that there was no statistically dose-response relationship between different transfusion products and the onset of HAI.

Conclusions: RBCs transfusion, platelets transfusion and FFPs transfusion were associated with HAI, but there was no dose-response relationship between them.
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http://dx.doi.org/10.1186/s13756-021-00928-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008558PMC
March 2021

Effectiveness and safety of ear acupuncture for allergic rhinitis: A protocol of randomized controlled trial.

Medicine (Baltimore) 2021 Mar;100(12):e24943

Ningbo Yinzhou District Baizhang Dongjiao Street Community Health Service Center.

Background: Allergic rhinitis is a global problem. About 10% to 40% of the global population is affected by allergic rhinitis and is on the rise, which has a significant health and economic impact on society. Ear acupuncture is a non-invasive acupuncture therapy, which has been used in the treatment of allergic rhinitis, and some positive results have been reported, but there is not enough evidence to prove its efficacy and safety.

Methods: This is a single-center, randomized, single-blind, sham-controlled trial. With the approval of the ethics committee of our hospital, participants with allergic rhinitis will be randomly assigned to receive either real or sham ear acupuncture once a week for 8 weeks, followed by 12 weeks of follow-up. Evaluate the patient's nasal symptom score and Standardised Rhinoconjunctivitis Quality of Life Questionnaire score, and monitor adverse events. Finally, the data are analyzed by SPSS 22.0 software.

Discussion: The results of this study will determine the efficacy and safety of ear acupuncture in the treatment of allergic rhinitis and provide a basis for promoting the application of ear acupuncture in the treatment of allergic rhinitis.

Trial Registration: OSF Registration number: DOI 10.17605/OSF.IO/MVEF7.
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http://dx.doi.org/10.1097/MD.0000000000024943DOI Listing
March 2021

Multidecadal records of microplastic accumulation in the coastal sediments of the East China Sea.

Chemosphere 2021 May 17;270:128658. Epub 2020 Oct 17.

Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-Sen University, Zhuhai, 519082, China; Southern Laboratory of Ocean Science and Engineering (Guangdong, Zhuhai), Zhuhai, 519000, China. Electronic address:

Microplastics are an emerging hazard in the marine environment, and considered to eventually sink into sediments. An investigation into the long-term variation of microplastic accumulation in sediment cores is essential for understanding the historical trend of this contamination and its response to human activities. In this study, the multidecadal changes of microplastic abundances in two sediment cores from the inner shelf of the East China Sea (ECS) were revealed by two methods, i.e., a visual enumeration method based on scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDS) and a quantitative method based on microplastic-derived carbon (MPC) abundances. The features of microplastics were determined via SEM-EDS and micro-Fourier transform infrared spectroscopy (μ-FTIR). The results reveal a multidecadal increasing trend of microplastic accumulation in the coastal sediments of the ECS since the 1960s, which may be jointly governed by the release of plastic wastes and oceanographic dynamics. Meanwhile, the breakpoint of the exponential growth of microplastics in the ECS occurs in 2000 AD, which well matches the rapid increasing of plastic production and consumption in China. Further, based on the MPC contents in sediments, the influence of microplastics on the quantitative evaluation of carbon storage in the ECS has been examined for the first time, revealing an insignificant (<2% before 2014 AD) but potentially-increasing (6.8% by 2025 AD) contribution of microplastics to carbon burial. Our results may provide the important data for evaluating and mitigating the impact of microplastics on the marine environment.
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http://dx.doi.org/10.1016/j.chemosphere.2020.128658DOI Listing
May 2021

Cyr61 mediates oxaliplatin resistance in colorectal cancer cells by regulating Bcl-xL expression.

J Cancer 2021 30;12(7):1952-1959. Epub 2021 Jan 30.

Department of Clinical Laboratory, Fujian Medical University Union Hospital, 29 Xinquan Road, Fuzhou, Fujian 350001, China.

Although the clinical application of oxaliplatin (L-OHP) has improved the survival of colorectal cancer (CRC) patients, approximately half of patients with CRC fail to achieve good clinical outcomes, indicating resistance to L-OHP therapy. Cysteine-rich protein 61 (Cyr61), a multifunctional extracellular matrix protein, is highly expressed in a variety of tumors; increased Cyr61 expression is known to be closely involved in the chemotherapeutic resistance of many tumors, but its role in the L-OHP resistance of CRC cells has not been studied. In this study, we aimed to investigate the role of Cyr61 in the L-OHP resistance of CRC cells and examine the underlying mechanism. Our findings showed that the mRNA and protein levels of Cyr61 in L-OHP-resistant cells were significantly increased compared with those in nonresistant cells. Knockdown of Cyr61 enhanced the chemosensitivity of L-OHP-resistant cells to L-OHP. Mechanistically, we found that overexpression of Cyr61 decreased L-OHP-induced apoptosis in drug-resistant CRC cells through the regulation of Bcl-xL. Collectively, our results revealed for the first time that Cyr61 plays a crucial role in the resistance of CRC cells to L-OHP and indicated that targeting Cyr61 may be a promising therapeutic strategy to overcome L-OHP resistance in CRC.
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http://dx.doi.org/10.7150/jca.48891DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7974528PMC
January 2021

Role of serum amitriptyline concentration and CYP2C19 polymorphism in predicting the response to low-dose amitriptyline in irritable bowel syndrome.

Dig Liver Dis 2021 Mar 19. Epub 2021 Mar 19.

Department of Gastroenterology, Guangzhou First People's Hospital, The Second Affiliated Hospital of South China University of Technology, Guangzhou, Guangdong Province , China.

Background: Low-dose amitriptyline (AMT) is an effective treatment for diarrhea-dominant irritable bowel syndrome (IBS-D). Its efficacy depends upon its serum concentration and the patient's CYP2C19 genotype.

Aims: To identify the association between serum AMT and nortriptyline (NT) concentration and CYP2C19 polymorphism and the clinical response in IBS-D patients.

Methods: Ninety IBS-D patients were treated of AMT for 6 weeks. Efficacy was evaluated by the results of the Adequate Relief question each week and an IBS severity scoring system (IBS-SSS) at 0, 3, and 6 weeks. CYP2C19 genotyping was performed by direct sequencing. AMT and NT steady-state serum concentrations were detected by high-performance liquid chromatography.

Results: The CYP2C19 polymorphism exhibited a significant influence on the NT serum concentration but did not predict the clinical efficacy of AMT for treating IBS-D. The NT steady-state and dose-corrected serum concentrations were significantly correlated with an improvement in the IBS-SSS score after 6 weeks, whereas the AMT serum concentration was not correlated with clinical improvement. The cut-off NT steady-state serum concentration of 2.91 ng/ml may help distinguish responders from non-responders.

Conclusions: NT serum concentration but not CYP2C19 polymorphism may be correlated with the clinical efficacy of AMT for treating IBS-D, and such a response may occur at the upper NT threshold of 2.91 ng/ml.
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http://dx.doi.org/10.1016/j.dld.2021.02.020DOI Listing
March 2021

Ectopic CD137 expression by rhabdomyosarcoma provides selection advantages but allows immunotherapeutic targeting.

Oncoimmunology 2021 02 4;10(1):1877459. Epub 2021 Feb 4.

Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.

Rhabdomyosarcoma (RMS) is a heterogeneous soft tissue neoplasm most frequently found in children and adolescents. As the prognosis for recurrent and metastatic RMS remains poor, immunotherapies are hoped to improve quality of life and survival. CD137 is a member of tumor necrosis factor receptor family and a T cell costimulatory molecule which induces potent cellular immune responses that are able to eliminate malignant cells. Therefore, it was puzzling to find expression of CD137 on an RMS tissue microarray by multiplex staining. CD137 is not only expressed by infiltrating T cells but also by malignant RMS cells. Functional in vitro experiments demonstrate that CD137 on RMS cells is being transferred to adjacent antigen-presenting cells by trogocytosis, where it downregulates CD137 ligand, and thereby reduces T cell costimulation which results in reduced killing of RMS cells. The transfer of CD137 and the subsequent downregulation of CD137 ligand is a physiological negative feedback mechanism that is likely usurped by RMS, and may facilitate its escape from immune surveillance. In addition, CD137 signals into RMS cells and induces IL-6 and IL-8 secretion, which are linked to RMS metastasis and poor prognosis. However, the ectopic expression of CD137 on RMS cells is an Achilles' heel that may be utilized for immunotherapy. Natural killer cells expressing an anti-CD137 chimeric antigen receptor specifically kill CD137-expressing RMS cells. Our study implicates ectopic CD137 expression as a pathogenesis mechanism in RMS, and it demonstrates that CD137 may be a novel target for immunotherapy of RMS.
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http://dx.doi.org/10.1080/2162402X.2021.1877459DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7872024PMC
February 2021

Autophagy activation and photoreceptor survival in retinal detachment.

Exp Eye Res 2021 Apr 18;205:108492. Epub 2021 Feb 18.

Department of Ophthalmology and Visual Sciences, University of Michigan, Kellogg Eye Center, Ann Arbor, MI, United States. Electronic address:

We assess the effect of autophagy inhibition on photoreceptor (PR) survival during experimental retinal detachment (RD) and examine the and examine the relationship between autophagy and the expression of glycolytic enzymes HK2 and PKM2 in the retina. We find that inhibiting autophagy by genetic knock out of the autophagy activator Atg5 in rod PRs resulted in increased apoptotic and necroptotic cell death during RD, demonstrated by elevated terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive cells, caspase 8 activity, transcript levels of Fas receptor and RIPK3 as compared to controls. The absence of autophagy in rods resulted in downregulation of hexokinase 2 and pyruvate kinase muscle isozyme 2 levels. More than 460 proteins were identified by mass spectroscopy in autophagosomes isolated from detached retinas compared with less than 150 proteins identified in autophagosomes from attached retinas. Among various cellular compartments, proteins from cytoskeleton, cytoplasm and intracellular organelles constituted a large portion of increased autophagosome contents. These proteins represent numerous biological processes, including phototransduction, cell-cell signaling, metabolism and inflammation. Our findings suggest that competent autophagy machinery is necessary for PR homeostasis and improving PR survival during periods of nutrient deprivation.
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http://dx.doi.org/10.1016/j.exer.2021.108492DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8043966PMC
April 2021

Metal-Organic Framework-Derived Hierarchical MnO/Co with Oxygen Vacancies toward Elevated-Temperature Li-Ion Battery.

ACS Nano 2021 Mar 19;15(3):4594-4607. Epub 2021 Feb 19.

Guangzhou Key Laboratory of Materials for Energy Conversion and Storage, Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, School of Chemistry, South China Normal University, Guangzhou 510006, China.

Transition metal oxides for high-temperature lithium-ion batteries have captivated orchestrated efforts for next-generation high-energy-density anodes. However, due to inherent low tap density, poor conductivity, and structural instability, their poor cyclability capacity and rate performance at elevated temperatures hinder further implementation. Oxygen vacancies (O) engineered by manipulating the active sites and electrical conductivity is a promising method for superior lithium storage. Herein, hierarchical MnO/Co nanoparticle-embedded -doped carbon nanotube (CNT)-assembled carbonaceous micropolyhedrons (O-MnO/Co NCPs) are constructed by a "4S" self-assembly, self-template, self-adaptive, and self-catalytic metal-organic framework template method with oxygen vacancies introduced. Impressively, the internal nanoparticles with metallic Co and the external -doped carbonaceous matrix entangled by fluffy self-generated CNTs synchronously constructed hierarchical micro/nano-secondary hybrids, facilitating highly compacted density, staggered conductive network, multidirectional diffusion pathways, and accelerated electrochemical kinetics. Experimental and density functional theory investigations systematically manifested that the O alongside the local built-in electric field within the crystal lattice induced the boosted electrical conductivity, additional active sites, and alleviated structural expansion, further achieving the exceptional diffusivity coefficient and pseudocapacitive capacity. Benefiting from the integrated structural and compositional optimization, the O-MnO/Co NCPs achieved distinguished "3C" performance with superior ultralong cyclability (a volumetric capacity of 1713.5 mAh cm at 1 A g up to 1000 cycles), good rate capacity (a well-maintained capacity of 670.2 mAh g even at 10 A g), and considerable high-temperature capability at 60 °C.
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http://dx.doi.org/10.1021/acsnano.0c08808DOI Listing
March 2021

Family veto in organ donation: the experiences of Organ and Tissue Donation Coordinators in Ontario.

Can J Anaesth 2021 May 11;68(5):611-621. Epub 2021 Feb 11.

Canadian Donation and Transplantation Research Program, Edmonton, AB, Canada.

Purpose: In Ontario, an individual's registered wish for organ donation is legally valid consent following death. Family veto occurs when the deceased donor's substitute decision-maker (SDM) overrides this consent to donate, evoking a legal and ethical conflict. The objective of this study was to examine the experiences of Organ and Tissue Donation Coordinators (OTDCs) working with SDMs who vetoed a deceased donor's consent for organ donation.

Methods: Qualitative focus groups were conducted with ten OTDCs in Ontario, Canada who reported experience with family veto. An interpretative phenomenological approach informed data analysis. Themes emerged through team consensus and were further refined through collaborative and reflexive engagement.

Results: Four themes emerged regarding family veto: 1) the significance of the OTDC role, 2) emotional distress and the "understandable" family veto, 3) barriers contributing to family veto, and 4) strategies towards a culture of organ donation. Findings highlighted the importance of patient advocacy in the OTDC role, while revealing the emotional distress of experiencing family veto. OTDCs identified timing and healthcare providers' perceived ambivalence toward organ donation as critical barriers to family authorization. Value-positive language, role reframing, and increased education were offered as strategies to address these barriers and reduce family veto.

Conclusion: This study highlights important considerations about organ donation authorization processes in Ontario. Findings support practice changes towards reducing family veto and further research nationally. Collaborations with key stakeholders are warranted to align healthcare practices, donation policies, and education initiatives towards a shared goal of increasing organ donation.
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http://dx.doi.org/10.1007/s12630-021-01928-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878166PMC
May 2021

Recent advances in proteome-wide label-free target deconvolution for bioactive small molecules.

Med Res Rev 2021 Feb 3. Epub 2021 Feb 3.

Department of Urology, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen Urology Minimally Invasive Engineering Center, Shenzhen, Guangdong, China.

Small-molecule drugs modulate biological processes and disease states through engagement of target proteins in cells. Assessing drug-target engagement on a proteome-wide scale is of utmost importance in better understanding the molecular mechanisms of action of observed beneficial and adverse effects, as well as in developing next generation tool compounds and drugs with better efficacies and specificities. However, systematic assessment of drug-target engagement has been an arduous task. With the continuous development of mass spectrometry-based proteomics instruments and techniques, various chemical proteomics approaches for drug target deconvolution (i.e., the identification of molecular target for drugs) have emerged. Among these, the label-free target deconvolution approaches that do not involve the chemical modification of compounds of interest, have gained increased attention in the community. Here we provide an overview of the basic principles and recent biological applications of the most important label-free methods including the cellular thermal shift assay, pulse proteolysis, chemical denaturant and protein precipitation, stability of proteins from rates of oxidation, drug affinity responsive target stability, limited proteolysis, and solvent-induced protein precipitation. The state-of-the-art technical implications and future outlook for the label-free approaches are also discussed.
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http://dx.doi.org/10.1002/med.21788DOI Listing
February 2021

Shentong Zhuyu Decoction Inhibits Inflammatory Response, Migration, and Invasion and Promotes Apoptosis of Rheumatoid Arthritis Fibroblast-like Synoviocytes via the MAPK p38/PPAR/CTGF Pathway.

Biomed Res Int 2021 15;2021:6187695. Epub 2021 Jan 15.

College of Integrated Chinese and Western Medicine, Hebei Medical University, Shijiazhuang, China.

Introduction: The current study is aimed at exploring the effect of Shentong Zhuyu Decoction on the proliferation, migration, invasion, and apoptosis of rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS) and its underlying molecular mechanism.

Materials And Methods: The type II collagen-induced arthritis (CIA) model was established. Subsequently, the RA-FLS were isolated from the CIA rat model and identified by immunohistochemistry. The viability, apoptosis, cell cycle, migration, and invasion of RA-FLS were detected by the cell counting kit 8 (CCK-8) assay, flow cytometry, wound-healing assay, and transwell invasion assay, respectively. The levels of MAPK p38, PPAR, CTGF, Bcl-2, Bax, caspase-3, IL-1, MMP-3, CDK4, and cyclin D1 were determined by qRT-PCR and western blotting, respectively.

Results: After treatment with Shentong Zhuyu Decoction medicated serum, the OD value, migrative and invasive abilities, and the secretion of IL-1, MMP-3 were remarkably decreased in RA-FLS, while the apoptosis rate was increased. Further, results showed that Shentong Zhuyu Decoction inhibited the transition from the G1 phase to S phase. Additionally, Shentong Zhuyu Decoction significantly inhibited the expression of Bcl-2, CDK4, cyclin D1, MAPK p-p38, and CTGF, whereas elevated the levels of Bax, caspase-3, and PPAR. Importantly, the effects of Shentong Zhuyu Decoction were consistent with the trends of MAPK P38 inhibitor (SB203580) and PPAR agonist (GW1929).

Conclusions: Shentong Zhuyu Decoction inhibited viability, inflammatory response, migration, invasion, and transition from the G1 phase to S phase and promoted apoptosis of RA-FLS via the MAPK p38/PPAR/CTGF pathway.
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http://dx.doi.org/10.1155/2021/6187695DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826240PMC
June 2021

Progress on genic male sterility gene in soybean.

Yi Chuan 2021 Jan;43(1):52-65

School of Life Sciences, Guangzhou University, Guangzhou 510006, China.

Male sterility refers to the phenomenon that stamens cannot grow normally and produce viable pollen grains in plants. Hybrid seed production by taking advantage of the trait of male sterility is an effective and quick strategy to increase crop yield. Up to date, the yield of rice (Oryza sativa L.), maize (Zea mays L.), wheat (Triticum aestivum L.) and other crops has been greatly increased based on hybrid vigor utilization. Soybean (Glycine max (L.) Merr.) is a self-pollination species, artificial emasculation is not only time-consuming, but also labor-intensive and economically impracticable. So far, large scale hybrid breeding has not been performed in soybean due to the shortage of male sterile lines suitable for hybrid production. Therefore, it is urgent to identify a stable male sterile system for the rapid utilization of heterosis in soybean. In this review, we summarize the progress on the discovery of soybean genic male sterility (GMS) mutants and GMS genes. Combining with the investigation of GMS genes in Arabidopsis, rice and maize, we provide important insights into the identification and potential utilization of GMS genes in soybean in the perspective of reverse genetics.
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http://dx.doi.org/10.16288/j.yczz.20-357DOI Listing
January 2021