Publications by authors named "Javier Nuñez"

59 Publications

Laparoscopic inguinal hernia repair: cost-effectiveness analysis of trend modifications of the technique.

Updates Surg 2021 Mar 3. Epub 2021 Mar 3.

Department of Advanced Medical and Surgical Sciences, Università Degli Studi Della Campania "Luigi Vanvitelli", Naples, Italy.

The aim of this study is to evaluate the cost-effectiveness of different modifications of the trans-abdominal pre-peritoneal (TAPP) repair of groin hernia. Data were collected prospectively for all consecutive patients who underwent TAPP unilateral inguinal hernia repair between November 2017 and March 2019, and who completed a minimum of 1 year of follow-up. Costs and quality adjusted life year (QALY) gained were collected. Three TAPP variations were assessed: mesh fixation and peritoneal closure with staples (group 1); mesh fixation with fibrin glue and peritoneal closure with sutures (group 2); and mesh fixation and peritoneal closure with fibrin glue (group 3). A matched group of open repairs was established. The incremental cost-effectiveness ratio (ICER) and main intra-operative and post-operative outcomes were assessed. Overall 120 patients were included (group 1 n = 31; group 2 n = 27; group 3 n = 33; open group: 29). Operative time was shorter for groups 2 and 3, and the main post-operative outcomes were similar. The overall mean total cost of the open group (1185.95€) was lower compared with the laparoscopic group (group 1: 1682.39; group 2: 1538.54€; group 3: 1510.1€) (p = 0.026). However, the mean ICERs of groups 2 and 3 were significantly higher compared with group 1 (p = 0.021) and the open group (p = 0.032). At simulations analysis, the probability of cost-effectiveness was 33.32%, 36.26%, and 36.7% in TAPP groups 1, 2, and 3. In the long term, laparoscopic repair of groin hernia is cost-effective compared with open surgery. The use of fibrin glue for mesh fixation and/or for closing the peritoneum is the most cost-effective option and shortens operative times.
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http://dx.doi.org/10.1007/s13304-021-01005-7DOI Listing
March 2021

Inertial flywheel knee- and hip-dominant hamstring strength exercises in professional soccer players: Muscle use and velocity-based (mechanical) eccentric overload.

PLoS One 2020 2;15(10):e0239977. Epub 2020 Oct 2.

Qatar Football Association, Doha, Qatar.

The primary aim of the present study was to analyze mechanical responses during inertial knee- and hip-dominant hamstring strengthening exercises (flywheel leg-curl and hip-extension in conic-pulley), and the secondary aim was to measure and compare regional muscle use using functional magnetic resonance imaging. Mean power, peak power, mean velocity, peak velocity and time in the concentric (CON) and eccentric (ECC) phases were measured. The transverse relaxation time (T2) shift from pre- to post-exercise were calculated for the biceps femoris long (BFl) and short (BFs) heads, semitendinosus (ST) and semimembranosus (SM) muscles at proximal, middle and distal areas of the muscle length. Peak and mean power in flywheel leg-curl were higher during the CON than the ECC phase (p<0.01). ECC peak power was higher than CON phase (p<0.01) in conic-pulley hip-extension exercise, while mean power was higher during the CON than ECC phase (p<0.01). Flywheel leg-curl showed a higher T2 values in ST and BFs and BFl (p<0.05), while the conic-pulley hip-extension had a higher T2 values in the proximal region of the ST and BFl (p<0.05). In conclusion, ECC overload was only observed in peak power during the conic-pulley hip-extension exercise. Flywheel leg-curl involved a greater overall use of the 4 muscle bellies, more specifically in the ST and BFs, with a selective augmented activity (compared with the conic-pulley) in the 3 regions of the BFs, while conic-pulley hip-extension exercise selectively targeted the proximal and medial regions of the BFl. Physiotherapists and strength and conditioning coaches should consider this when optimizing the training and recovery process for hamstring muscles, especially after injury.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0239977PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7531833PMC
November 2020

Periodontal regeneration using a xenogeneic bone substitute seeded with autologous periodontal ligament-derived mesenchymal stem cells: A 12-month quasi-randomized controlled pilot clinical trial.

J Clin Periodontol 2020 11;47(11):1391-1402

ETEP (Etiology and Therapy of Periodontal and Peri-implant Diseases) Research Group, University Complutense, Madrid, Spain.

Aim: To evaluate the safety and efficacy of autologous periodontal ligament-derived mesenchymal stem cells (PDL-MSCs) embedded in a xenogeneic bone substitute (XBS) for the regenerative treatment of intra-bony periodontal defects.

Material And Methods: This quasi-randomized controlled pilot phase II clinical trial included patients requiring a tooth extraction and presence of one intra-bony lesion (1-2 walls). Patients were allocated to either the experimental (XBS + 10 × 10 PDL-MSCs/100 mg) or the control group (XBS). Clinical and radiographical parameters were recorded at baseline, 6, 9 and 12 months. The presence of adverse events was also evaluated. Chi-square, Student's t test, Mann-Whitney U, repeated-measures ANOVA and regression models were used.

Results: Twenty patients were included. No serious adverse events were reported. Patients in the experimental group (n = 9) showed greater clinical attachment level (CAL) gain (1.44, standard deviation [SD] = 1.87) and probing pocket depth (PPD) reduction (2.33, SD = 1.32) than the control group (n = 10; CAL gain = 0.88, SD = 1.68, and PPD reduction = 2.10, SD = 2.46), without statistically significant differences.

Conclusion: The application of PDL-MSCs to XBS for the treatment of one- to two-wall intra-bony lesions was safe and resulted in low postoperative morbidity and appropriate healing, although its additional benefit, when compared with the XBS alone, was not demonstrated.
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http://dx.doi.org/10.1111/jcpe.13368DOI Listing
November 2020

Strength Conditioning Program to Prevent Adductor Muscle Strains in Football: Does it Really Help Professional Football Players?

Int J Environ Res Public Health 2020 09 2;17(17). Epub 2020 Sep 2.

Department of Sports and Informatics, Sport Faculty, University of Pablo de Olavide of Sevilla, 41013 Sevilla, Spain.

Coaches at the professional level are often concerned about negative side effects from testing and intensive resistance training periods, and they are not willing to base their training prescriptions on data obtained from semiprofessional or amateur football players. Consequently, the purpose of this study was to analyze the reliability and effectiveness of two adductor injury active prevention programs using the adductor/abductor ratio and deficit between legs, on the basis of adduction-abduction power output during the exercises proposed, in professional football players. Forty-eight professional football players undertook complementary strength training for the adductor and abductor muscles in their dominant and non-dominant legs, once or twice a week throughout the playing season. The volume of the session was determined by the adductor/abductor ratio and the deficit between legs in the last session training measured. The number and severity of muscle injuries per 1000 h of exposure were recorded. Both prevention programs showed a very low rate of adductor injury (0.27 and 0.07 injuries/1000 h) with mild-to-moderate severity, maintaining a balance in percentage asymmetry between dominant and non-dominant legs for adductor (10.37%) and in the adductor/abductor ratio (0.92) in top professional football players throughout the season. The strength conditioning program proposed can help to prevent adductor muscle injuries in top professional football players.
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http://dx.doi.org/10.3390/ijerph17176408DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7504263PMC
September 2020

Hard and soft tissue changes after guided bone regeneration using two different barrier membranes: an experimental in vivo investigation.

Clin Oral Investig 2021 Apr 26;25(4):2213-2227. Epub 2020 Aug 26.

Postgraduate Periodontology Clinic, Faculty of Odontology, University Complutense of Madrid, Madrid, Spain.

Objective: To assess the contour and volumetric changes of hard and soft tissues after guided bone regeneration (GBR) using two types of barrier membranes together with a xenogeneic bone substitute in dehiscence-type defects around dental implants.

Material And Methods: In 8 Beagle dogs, after tooth extraction, two-wall chronified bone defects were developed. Then, implants were placed with a buccal dehiscence defect that was treated with GBR using randomly: (i) deproteinized bovine bone mineral (DBBM) covered by a synthetic polylactic membrane (test group), (ii) DBBM plus a porcine natural collagen membrane (positive control) and (iii) defect only covered by the synthetic membrane (negative control group). Outcomes were evaluated at 4 and 12 weeks. Micro-CT was used to evaluate the hard tissue volumetric changes and STL files from digitized cast models were used to measure the soft tissues contour linear changes.

Results: Test and positive control groups were superior in terms of volume gain and contour changes when compared with the negative control. Soft tissue changes showed at 4 weeks statistically significant superiority for test and positive control groups compared with negative control. After 12 weeks, the results were superior for test and positive control groups but not statistically significant, although, with a lesser magnitude, the negative control group exhibited gains in both, soft and hard tissues.

Conclusions: Both types of membranes (collagen and synthetic) attained similar outcomes, in terms of hard tissue volume gain and soft tissue contours when used in combination with DBBM CLINICAL RELEVANCE: Synthetic membranes were a valid alternative to the "gold standard" natural collagen membrane for treating dehiscence-type defects around dental implants when used with a xenogeneic bone substitute scaffold.
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http://dx.doi.org/10.1007/s00784-020-03537-5DOI Listing
April 2021

Inline Infrared Chemical Identification of Particulate Matter.

Sensors (Basel) 2020 Jul 28;20(15). Epub 2020 Jul 28.

The Netherlands Organisation for Applied Scientific Research, HTC25, 5656AE Eindhoven, The Netherlands.

The health and environmental effects of particulate matter (PM) in the air depend on several parameters. Besides particle size, shape, and concentration, the chemical nature of the PM is also of great importance. State-of-the-art PM sensors only detect the particle size and concentration. Small, low-cost sensors only identify PM according to PM2.5 and PM10 standards. Larger detectors measure the complete particle size distribution. However, the chemical composition of PM is not often assessed. The current paper presents the initial stages of the development of an infrared-based detector for the inline assessment of the chemistry of PM in the air. By combining a mini cyclone that is able to concentrate the particles at least a thousand fold and a hollow waveguide that aligns the flow of particles with infrared light, the feasibility of the concept was shown in this study. A clear differentiation between amorphous and crystalline silica was demonstrated at outdoor PM levels of lower than 1 mg per cubic meter.
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http://dx.doi.org/10.3390/s20154193DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7435449PMC
July 2020

Early Prediction of Subsequent Molecular Response to Nilotinib in Patients with Chronic Myeloid Leukemia: Comparison of the Quantification of BCR-ABL1 Ratios Using ABL1 or GUSB Control Genes.

J Mol Diagn 2020 10 17;22(10):1217-1224. Epub 2020 Jul 17.

Department of Hematology, Hospital de la Princesa, Madrid, Spain. Electronic address:

Molecular monitoring of BCR-ABL1 transcripts is a critical prognostic indicator of treatment response in chronic myeloid leukemia (CML). Quantification of BCR-ABL1 transcripts using ABL1 or GUSB as control genes on the early molecular response (MR) to frontline nilotinib was studied using data from 60 patients with chronic-phase CML from the Evaluating Nilotinib Efficacy and Safety in Clinical Trials as First-Line Treatment (ENEST1st) substudy. Effects of BCR-ABL1/ABL1 and BCR-ABL1/GUSB ratios at early time points as independent variables on subsequent MR were determined by logistic regression analyses and predictive cut-off values determined by receiver operating curve analyses. From day 45, concordance was found for both control genes' early transcript kinetics and ability to predict subsequent deep MR at 18 months. From baseline to 3 months, transcripts descended linearly with both control genes. Use of ABL1 allowed for an earlier prediction (2 months) of subsequent MR than with GUSB (3 months), with cut-off values of 1.5% and 0.19%, respectively. The dynamic determination of BCR-ABL1 transcripts using either internal control gene is valid and predictive of subsequent MR. The use of GUSB to predict an earlier and more accurate response than ABL1 is not supported in the results. Accurate early indicators of MR are essential to identify patients likely to have inferior outcomes who may benefit from treatment with an alternative tyrosine kinase inhibitor.
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http://dx.doi.org/10.1016/j.jmoldx.2020.06.016DOI Listing
October 2020

Bilateral Deficit and Bilateral Performance: Relationship with Sprinting and Change of Direction in Elite Youth Soccer Players.

Sports (Basel) 2020 Jun 3;8(6). Epub 2020 Jun 3.

Physical Performance and Sport Research, Pablo de Olavide University, 41013 Sevilla, Spain.

The purpose of the study was to examine the differences in bilateral deficit (BLD) at different loadings during the half-squat jump (SJ) and horizontal countermovement jump (HCMJ) to determine if there is a relationship with linear sprint or change of direction (COD). The second goal was to check if fast players were more powerful in SJ and HCMJ than slow players in bilateral performance (BP). Twenty-seven male youth soccer players participated in the study. Players were divided in two groups, faster and slower, according to their sprint performance (10 and 40 m). BLD average power with body weight (BW) and 25%BW were significantly higher than 50%BW ( < 0.01). BLD during HCMJ was significantly higher than BLD during SJ with BW, 25%BW and 50%BW ( < 0.01). There were no statistical relationships between BLD and sprint or COD performance ( > 0.05). Fast players showed significantly higher SJ power with all the different loads and HCMJ than slow players ( < 0.01), and fast players lost more time executing COD-90° than slow players ( < 0.01). There were no statistical differences between fast and slow players in BLD. BLD seems to be dependent on motor task, contraction type and load and could not be a proper measure to estimate sprint and COD performance. Faster players are confirmed to be more powerful players than slow players, and decrements in COD could be a key benchmark to identify deficit between linear and COD performance.
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http://dx.doi.org/10.3390/sports8060082DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7353648PMC
June 2020

Robotic-assisted right colectomy versus laparoscopic approach: case-matched study and cost-effectiveness analysis.

J Robot Surg 2021 Feb 4;15(1):115-123. Epub 2020 May 4.

Division of General Surgery, HM-Sanchinarro University Hospital, San Pablo University, calle oña 10, Madrid, Spain.

Aim: The aim of this study is to compare clinical and oncological outcomes of robot-assisted right colectomy with those of conventional laparoscopy-assisted right colectomy, reporting for the first time in literature, a cost-effectiveness analysis.

Methods: This is a case-matched prospective non-randomized study conducted from October 2013 to October 2017 at Sanchinarro University Hospital, Madrid. Patients with right-sided colonic adenocarcinoma or adenoma, not suitable endoscopic resection were treated with robot-assisted right colectomy and a propensity score-matched (1:1) was used to balance preoperative characteristics of a laparoscopic control group. Perioperative, postoperative, long-term oncological results and costs were analysed, and quality-adjusted life years (QALY), and the cost-effectiveness ratio (ICER) were calculated. The primary end point was to compare the cost-effectiveness differences between both groups. A willingness-to-pay of 20,000 and 30,000 per QALY was used as a threshold to recognize which treatment was most cost effective.

Results: Thirty-five robot-assisted right colectomies were included and a group of 35 laparoscopy-assisted right colectomy was selected. Compared with the laparoscopic group, the robotic group was associated with longer operation times (243 min vs. 179 min, p < 0.001). No significant difference was observed in terms of total costs between the robotic and laparoscopic groups (9455.14 vs 8227.50 respectively, p = 0.21). At a willingness-to-pay threshold of 20,000 and 30,000, there was a 78.78-95.04% probability that the robotic group was cost effective relative to laparoscopic group.

Conclusion: Robot-assisted right colectomy is a safe and feasible technique and is a cost-effective procedure.
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http://dx.doi.org/10.1007/s11701-020-01084-5DOI Listing
February 2021

Is possible an eccentric overload in a rotary inertia device? Comparison of force profile in a cylinder-shaped and a cone-shaped axis devices.

J Sports Sci 2020 Jul 17;38(14):1624-1628. Epub 2020 Apr 17.

Physical Performance & Sports Research, Universidad Pablo de Olavide , Seville, Spain.

The aims of this study were to compare the force profile of using a horizontal cylinder-shaped axis or a vertical cone-shaped axis to provide resistance in rotary inertia devices, and to report the evolution of kinetic and kinematic variables in experienced athletes during a half-squat exercise. Twenty-two healthy active men participated in the assessment of time, peak velocity, peak force, time to reach the peak force, average force, impulse, and range of movement, during a half-squat incremental test performed on conical inertial device (CP) and on cylinder inertial device (YY). The analysis showed that YY during CON-ECC phased generates substantial higher peak_force, mean_force, impulse, time, and a lower peak_velocity, than CP. We never obtained eccentric overload for peak_force or mean_force. CP offers less resistance to accelerate-decelerate the movement with respect to YY, we need checking whether eccentric overload it is being produced, and the impulse was the only kinetic variable that was able to discriminate between the inertias and devices.
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http://dx.doi.org/10.1080/02640414.2020.1754111DOI Listing
July 2020

Relationships between Change of Direction, Sprint, Jump, and Squat Power Performance.

Sports (Basel) 2020 Mar 19;8(3). Epub 2020 Mar 19.

Physical Performance and Sport Research, Pablo de Olavide University, 41013 Sevilla, Spain.

The aim of the study was to investigate the relationships between countermovement jump (CMJ) height and inertial power in squat and sprint variables with change of direction (COD) performance. Fifty young healthy active males participated in the study. To determine these relationships, we carried out a 10-m linear sprint test (T 10 m), vertical jump tests (CMJ and CMJ Abalakov), an assessment of power relative to bodyweight in a flywheel squat (P), and 10-m COD sprints with two different turn types (COD-90° and COD-180°). T10 m showed statistically large and moderate correlations with T10 m COD-180° (r = 0.55) and T10-m COD-90° (r = 0.41), respectively. Moderate to large correlations between jumping height, linear sprinting, and sprints with COD were found (r = -0.43 to r = -0.59), and there were unclear correlations between jumping height and the loss of speed caused by executing COD (DEC-COD). P showed a large correlation with CMJ Abalakov and CMJ jump height (r = 0.65 and r = 0.57, respectively), and a moderate and large correlation with T 10 m, T 10 m COD-180°, and T10 m COD-90° (r = -0.33, r = -0.38, and r = -0.54, respectively). Despite the existence of substantial correlations between variables, straight linear sprinting, jumping performance, CODs and squat power were, for the most part, separate motor qualities (R from 14% to 34%), suggesting that all of them should be specifically assessed and trained.
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http://dx.doi.org/10.3390/sports8030038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7183057PMC
March 2020

Validity of Field Methods to Estimate Fat-Free Mass Changes Throughout the Season in Elite Youth Soccer Players.

Front Physiol 2020 12;11:16. Epub 2020 Feb 12.

Physical Performance and Sports Research, Pablo de Olavide University, Seville, Spain.

The aim of this study was to determine the most effective anthropometric equations or bioelectrical impedance analysis (BIA) devices for quantifying the sensitivity to change in fat-free mass (FFM) in elite young soccer players, in comparison with measurements using dual-energy X-ray absorptiometry (DXA), between the pre- and mid-season. A total of 40 elite youth soccer players participated in this study. DXA values provided a criterion measure of FFM. Correlation coefficients, biases, limits of agreement, and differences were used as measures of sensitivity to change. All body density, skinfold, and anthropometric equations and BIA devices used to obtain FFM data showed positive and very large correlations ( from 0.70 to 0.89) with DXA. A significant increase in FFM was shown between time points using DXA, BIA, and all anthropometric equations ( < 0.01). The magnitudes of differences were small for DXA, BIA inbody and all anthropometric equations except those of Faulkner (1966), Durnin and Rahaman (1967), Brook (1971), and Sarría et al. (1998). Six anthropometric equations [Faulkner (1966), Durnin and Womersley (1974), Carter (1982), Slaughter et al. (1988), Reilly et al. (2009), and Munguia-Izquierdo et al. (2018)] and BIA Tanita showed no statistical differences compared to DXA, with a low bias. We concluded that the equations developed by Durnin and Womersley (1974), Carter (1982), Slaughter et al. (1988), Reilly et al. (2009), and Munguia-Izquierdo et al. (2018) showed the best sensitivity in assessing FFM changes between pre- and mid-season in elite youth soccer players.
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http://dx.doi.org/10.3389/fphys.2020.00016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7029743PMC
February 2020

Remodeling of Intracellular Ca Homeostasis in Rat Hippocampal Neurons Aged In Vitro.

Int J Mol Sci 2020 Feb 24;21(4). Epub 2020 Feb 24.

Institute of Biology and Molecular Genetics (IBGM), University of Valladolid and National Research Council (CSIC), 47003 Valladolid, Spain.

Aging is often associated with a cognitive decline and a susceptibility to neuronal damage. It is also the most important risk factor for neurodegenerative disorders, particularly Alzheimer's disease (AD). AD is related to an excess of neurotoxic oligomers of amyloid β peptide (Aβo); however, the molecular mechanisms are still highly controversial. Intracellular Ca homeostasis plays an important role in the control of neuronal activity, including neurotransmitter release, synaptic plasticity, and memory storage, as well as neuron cell death. Recent evidence indicates that long-term cultures of rat hippocampal neurons, resembling aged neurons, undergo cell death after treatment with Aβo, whereas short-term cultures, resembling young neurons, do not. These in vitro changes are associated with the remodeling of intracellular Ca homeostasis with aging, thus providing a simplistic model for investigating Ca remodeling in aging. In vitro aged neurons show increased resting cytosolic Ca concentration, enhanced Ca store content, and Ca release from the endoplasmic reticulum (ER). Ca transfer from the endoplasmic reticulum (ER) to mitochondria is also enhanced. Aged neurons also show decreased store-operated Ca entry (SOCE), a Ca entry pathway related to memory storage. At the molecular level, in vitro remodeling is associated with changes in the expression of Ca channels resembling in vivo aging, including changes in N-methyl-D-aspartate NMDA receptor and inositol 1,4,5-trisphosphate (IP) receptor isoforms, increased expression of the mitochondrial calcium uniporter (MCU), and decreased expression of Orai1/Stim1, the molecular players involved in SOCE. Additionally, Aβo treatment exacerbates most of the changes observed in aged neurons and enhances susceptibility to cell death. Conversely, the solely effect of Aβo in young neurons is to increase ER-mitochondria colocalization and enhance Ca transfer from ER to mitochondria without inducing neuronal damage. We propose that cultured rat hippocampal neurons may be a useful model to investigate Ca remodeling in aging and in age-related neurodegenerative disorders.
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http://dx.doi.org/10.3390/ijms21041549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073228PMC
February 2020

Use of Phycobiliproteins from Atacama Cyanobacteria as Food Colorants in a Dairy Beverage Prototype.

Foods 2020 Feb 24;9(2). Epub 2020 Feb 24.

Departamento Biomédico, Laboratorio de Bioquímica, Facultad de Ciencias de la Salud and Centre for Biotechnology and Bioengineering (CeBiB), Universidad de Antofagasta, Avenida Angamos N° 601, Antofagasta 1270300, Chile.

The interest of the food industry in replacing artificial dyes with natural pigments has grown recently. Cyanobacterial phycobiliproteins (PBPs), phycoerythrin (PE) and phycocyanin (PC), are colored water-soluble proteins that are used as natural pigments. Additionally, red PE and blue PC have antioxidant capabilities. We have formulated a new food prototype based on PBP-fortified skim milk. PBPs from Andean cyanobacteria were purified by ammonium sulfate precipitation, ion-exchange chromatography, and freeze-drying. The stability of PE and PC was evaluated by changes in their absorption spectra at various pH (1-14) and temperature (0-80 °C) values. Purified PBPs showed chemical stability under pH values of 5 to 8 and at temperatures between 0 and 50 °C. The antioxidant property of PBP was confirmed by ABTS (2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical ion scavenging, and FRAP (Ferric Antioxidant Power) assays. The absence of PBP toxicity against was confirmed up to 1 mg PBP/mL. Skim milk fortified with PE obtained a higher score after sensory tests. Thus, a functional food based on skim milk-containing cyanobacterial PBPs can be considered an innovative beverage for the food industry. PBPs were stable at an ultra-high temperature (138 °C and 4 s). PBP stability improvements by changes at its primary structure and the incorporation of freeze-dried PBPs into sachets should be considered as alternatives for their future commercialization.
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http://dx.doi.org/10.3390/foods9020244DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074303PMC
February 2020

Eccentric-concentric Ratio: A Key Factor for Defining Strength Training in Soccer.

Int J Sports Med 2019 Nov 21;40(12):796-802. Epub 2019 Aug 21.

Faculty of Health Sciences, University of San Jorge, Zaragoza, Spain.

The aims of this study were to analyse the effect of chronic strength training over concentric power (CON), eccentric power (ECC), ECC/CON ratio, and 20 m linear sprint performance in elite young soccer players. Twenty young elite Spanish soccer players were assigned to an experimental group (CPG) which performed a front-step exercise using a conical pulley, 2-3 sets of 6 repetitions each leg, during 9 weeks (CPG, n=10) in addition to its usual strength training, or to a control group (CG, n=10). The improvements in the ECC mean power (36%, ES=1.61), and ECC / CON ratio (17%, ES=1.77) were substantially greater in the CPG than in the CG while the CON mean power (16%, ES=0.83) was substantially greater in the CG than in the CPG. The sprinting time for 10 m (2.8%, ES=0.78) and the 10 m flying time between 10-20 m (1.72%, ES=0.41) were substantially enhanced in CPG and CG respectively. To be efficient when defining a functional strength training and performance increments using an inertial device, the mean power output need to be measured during the CON and ECC phases and an analysis of the ECC / CON ratio should be included.
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http://dx.doi.org/10.1055/a-0977-5478DOI Listing
November 2019

Field Methods to Estimate Fat-free Mass in International Soccer Players.

Int J Sports Med 2019 Sep 31;40(10):619-624. Epub 2019 Jul 31.

University of Pablo de Olavide, Physical Performance & Sports Research, Seville, Spain.

Based on the high financial and logistical costs associated with the assessment of body composition with dual-energy X-ray absorptiometry (DXA), this study determined which field method has the best correlation with DXA data, and developed an equation to estimate fat-free mass (FFM) using the field anthropometric data in international soccer players. A total of 17 international soccer players participated in this study. DXA values provided a criterion measure of FFM. Correlation coefficients, biases, limits of agreement, and differences were used as validity measures, and regression analyses were used to develop the prediction equation. All field methods used to obtain FFM data showed positive correlations (r from 0.90-0.96) with DXA. Only the equation developed by Deurenberg et al. [6] showed no differences from DXA with a low bias. The main strength of this study was providing a valid and accurate equation to estimate FFM specifically in international soccer players.
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http://dx.doi.org/10.1055/a-0969-8591DOI Listing
September 2019

Who Differentiates by Skin Color? Status Attributions and Skin Pigmentation in Chile.

Front Psychol 2019 3;10:1516. Epub 2019 Jul 3.

Department of Economics, Faculty of Economics and Business, Universidad de Chile, Santiago, Chile.

A growing body of research has shown that phenotypes and skin pigmentation play a fundamental role in stratification dynamics in Latin American countries. However, the relevance of skin color on status attribution for different status groups has been little studied in the region. This article seeks to broaden the research on phenotypic status cues using Chile as a context for analysis - a Latin American country with a narrow although continuous spectrum of skin tones, marked status differences, and a mostly white elite. We draw on status construction theory to hypothesize that skin pigmentation in Chile has become a status cue, although its heuristic relevance could differ across status groups. Using visual stimuli and a repeated measure design, we studied this relationship and tested whether the use of skin pigmentation as a status cue is conditional upon the status of those categorizing others. The results reveal that participants attribute, on average, lower status to others of darker skin. Besides, skin pigmentation has a conditional effect on the social status of participants: whereas skin pigmentation does not work as a status cue for lower status participants, it is an important status marker for the categorizations that middle and especially higher status participants perform. The phenotypic composition of reference groups of low- and high-status individuals and system justification are discussed as potential explanations for these results.
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http://dx.doi.org/10.3389/fpsyg.2019.01516DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6618139PMC
July 2019

Accurate Phylogenetic Relationships Among Strains Circulating in France Based on Whole Genome Sequencing and Single Nucleotide Polymorphism Analysis.

Front Microbiol 2019 3;10:955. Epub 2019 May 3.

ISP, INRA, UMR 1282, Université de Tours, Nouzilly, France.

In recent years the diversity of the French population responsible for bovine tuberculosis (bTB) outbreaks since 1970 has been described in detail. To further understand bTB evolution in France, we used single nucleotide polymorphisms (SNPs) based on whole genome sequence versus classical genotyping methods in order to identify accurate phylogenetic relationships between strains. Whole genome sequencing was carried out on a selection of 87 strains which reflect the French population's genetic diversity. Sequences were compared to the reference genome AF2122/97. Comparison among the 87 genomes revealed 9,170 sites where at least one strain shows a SNP with respect to the reference genome; 1,172 are intergenic and 7,998 in coding sequences, of which 2,880 are synonymous and 5,118 non-synonymous. SNP-based phylogenetic analysis using these 9,170 SNP is congruent with the cluster defined by spoligotyping and multilocus variable number of tandem repeat analysis typing. In addition, some SNPs were identified as specific to genotypic groups. These findings suggest new SNP targets that can be used for the development of high-resolving methods for genotyping as well as for studying evolution and transmission patterns. The detection of non-synonymous SNPs on virulence genes enabled us to distinguish different clusters. Our results seem to indicate that genetically differentiated clusters could also display distinctive phenotypic traits.
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http://dx.doi.org/10.3389/fmicb.2019.00955DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6509552PMC
May 2019

Cellular therapy in periodontal regeneration.

Periodontol 2000 2019 02;79(1):107-116

Faculty of Odontology, Complutense University of Madrid, Madrid, Spain.

Periodontitis is a chronic inflammatory condition leading to destruction of the tooth supporting tissues, which if left untreated may cause tooth loss. The treatment of periodontitis mainly aims to arrest the inflammatory process by infection control measures, although in some specific lesions a limited periodontal regeneration can also be attained. Current regenerative approaches are aimed to guide the cells with regenerative capacity to repopulate the lesion and promote new cementum and new connective tissue attachment. The first phase in periodontal tissue regeneration involves the differentiation of mesenchymal cells into cementoblasts to promote new cementum, thus facilitating the attachment of new periodontal ligament fibers to the root and the alveolar bone. Current regenerative approaches limit themselves to the confines of the lesion by promoting the self-regenerative potential of periodontal tissues. With the advent of bioengineered therapies, several studies have investigated the potential use of cell therapies, mainly the use of undifferentiated mesenchymal cells combined with different scaffolds. The understanding of the origin and differentiation patterns of these cells is, therefore, important to elucidate their potential therapeutic use and their comparative efficacy with current technologies. This paper aims to review the in vitro and experimental studies using cell therapies based on application of cementoblasts and mesenchymal stem cells isolated from oral tissues when combined with different scaffolds.
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http://dx.doi.org/10.1111/prd.12250DOI Listing
February 2019

Ridge alterations after implant placement in fresh extraction sockets or in healed crests: An experimental in vivo investigation.

Clin Oral Implants Res 2019 Apr 3;30(4):353-363. Epub 2019 Apr 3.

Section of Periodontology, Faculty of Odontology, University Complutense of Madrid, Madrid, Spain.

Objectives: To study the early and late bone healing around implants placed according to a flapless immediate (test) or flapped delayed (control) implant surgical protocol.

Materials And Methods: In eight beagle dogs, immediate and delayed implants were placed. Test and control implants were randomly assigned, and horizontal and vertical bone remodelling, as well as bone-to-implant contact (BIC), were assessed with histology at 2 and 8 weeks. Teeth were used as negative controls.

Results: Similar results were attained in the two groups when the vertical resorption of the buccal bone crest was evaluated (approximately 0.5 mm). On the other hand, a marked horizontal buccal bone resorption was observed. Immediate implants attained a thinner buccal bone crest as compared to the delayed implant and the tooth. De novo bone formation on the implant surface appeared to be more pronounced at delayed sites at 2 weeks, whereas at 8 weeks, no difference in BIC was observed.

Conclusions: Ridge alterations occurred at both implant placement protocols, with similar limited amounts of vertical buccal bone reduction and a pronounced horizontal bone reduction. The process of horizontal bone remodelling differed between the two surgical protocols.
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http://dx.doi.org/10.1111/clr.13421DOI Listing
April 2019

A novel methodological approach using superimposed Micro-CT and STL images to analyze hard and soft tissue volume in immediate and delayed implants with different cervical designs.

Clin Oral Implants Res 2018 Oct 23;29(10):986-995. Epub 2018 Sep 23.

Section of Periodontology, Faculty of Odontology, University Complutense of Madrid, Madrid, Spain.

Objectives: To study the hard and soft tissue volume after placing immediate (IMI) or delayed implants (DLI) with a triangular coronal macro-design (Test/T) or a conventional cylindrical design (Control/C).

Material And Methods: T/C implants were inserted in healed ridges or in fresh extraction sockets of eight beagle dogs. Biopsies were processed for Micro-CT analysis and dental stone casts were optically scanned to obtain STL files revealing the soft tissue contours at 12 weeks. Image analysis software was utilized to match common landmarks superimposing the two sets of data. Three distinct volumes were calculated; buccal bone volume (B-BV), soft tissue volume below the implant shoulder (EC-STV), and the soft tissue volume above the implant shoulder (SC-STV). Using linear measurements, the soft tissue height (STH), the mucosal thickness (MT-IS), and the distance from the implant shoulder to the bone crest (I-BC) were assessed in the digital images and in conventional histology to assess the concordance, reproducibility, and reliability.

Results: There were no significant differences between test and control implants regarding the buccal bone volume, although test implants had greater B-BV in all locations except for PM2. The soft tissue volume was similar at T/C implants. The surgical approach influenced the distribution of the total tissue volume. In the IMI, a low position of the bone crest was correlated with low values of B-BV, SC-STV, MT-IS, and STH. Linear measurements showed a high correlation between the histology and digital measurements and high inter and intra examiner agreement.

Conclusion: The superimposition of Micro-CT/STL allowed the analysis of soft and hard tissue volumes. Reduction of the implant buccal aspect resulted in nonsignificant higher bone volume although similar soft tissue volume while the surgical approach influenced soft tissue response.
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http://dx.doi.org/10.1111/clr.13365DOI Listing
October 2018

A Two-Step Surgical Approach with Flattening of the Root Surface to Treat Localized Gingival Recessions Affecting Mandibular Incisors: A Case Series Report.

Int J Periodontics Restorative Dent 2018 Sep/Oct;38(5):683-690

Root coverage in the anterior mandible is challenging due to a thin gingiva, shallow vestibule, and/or high frenulum. This case series reports on the flattening of the root surface to create a new emergence profile conceived with a two-step surgical approach aimed at providing more space for the graft, increasing the thickness of the gingival margin, and getting extra soft tissue in the open area of the recession. A total of 10 patients with recessions affecting the mandibular incisors were treated to evaluate this two-step approach, which included odontoplasty of the root followed by a connective tissue graft. At 1 year, the mean coverage was 100% in Class II recessions, and 80.5% in Class III. The mean keratinized tissue increase was 5.80 ± 1.75 mm. This surgical approach could be proposed as an alternative when treating mandibular anterior teeth with root prominence or with a buccally tilted position.
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http://dx.doi.org/10.11607/prd.3566DOI Listing
February 2019

Effect of different oxidative stress degrees generated by hydrogen peroxide on motility and DNA fragmentation of zebrafish (Danio rerio) spermatozoa.

Reprod Domest Anim 2018 Dec 29;53(6):1498-1505. Epub 2018 Aug 29.

Departament de Biologia Cellular, Biologia Funcional i Antropologia Fisica. Universitat de València, Burjassot, Spain.

An increase in reactive oxygen species (ROS) or decrease in antioxidant barriers can provoke lipid peroxidation of the membranes or DNA damage of the spermatozoa. The aim of this work is to study the effect of the different degrees of oxidative stress generated by H O incubation on total motility, kinetics, and DNA fragmentation of zebrafish (Danio rerio) spermatozoa. For this process, experimental groups were incubated in 50 µM (Low; L) and 200 µM (High; H) H O , respectively, for 20 min at 4ºC. Sperm motility parameters were obtained with a computer-assisted sperm analysis (CASA) system. Sperm DNA fragmentation (SDF) was assessed using the sperm chromatin dispersion test. Both low and high H O concentration groups showed lower motility than control groups. Progressive motility of spermatozoa incubated in the H group dropped rapidly in comparison with other groups. Regarding SDF, the control and L groups had significantly lower values than the H group (25.0% and 31.6% vs. 48.1% fragmented sperm for C, L, and H groups, respectively; p < 0.05). Sperm motility, mostly progressive motility, decreased as H O concentration increased, mainly when time after sperm activation increased. SDF increased as the H O concentration increased. However, measurements of the halo area did not agree with the subjective SDF rate.
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http://dx.doi.org/10.1111/rda.13296DOI Listing
December 2018

Cell therapy with allogenic canine periodontal ligament-derived cells in periodontal regeneration of critical size defects.

J Clin Periodontol 2018 04 29;45(4):453-461. Epub 2018 Jan 29.

Section of Periodontology, Faculty of Odontology, University Complutense of Madrid, Madrid, Spain.

Aim: The objective of this in vivo experimental study to evaluate the regenerative potential of a cell therapy combining allogenic periodontal ligament-derived cells within a xenogeneic bone substitute in a similar experimental model.

Methods: In nine beagle dogs, critical size 6-mm supra-alveolar periodontal defects were created around the PIII and PIV. The resulting supra-alveolar defects were randomly treated with either 1.4 × 10 allogenic canine periodontal ligament-derived cells seeded on de-proteinized bovine bone mineral with 10% collagen (DBBM-C) (test group) or DBBM-C without cells (control group). Specimens were obtained at 3 months, and histological outcomes were studied.

Results: The histological analysis showed that total furcation closure occurred very seldom in both groups, being the extent of periodontal regeneration located in the apical third of the defect. The calculated amount of periodontal regeneration at the furcation area was comparable in both the test and control groups (1.93 ± 1.14 mm (17%) versus 2.35 ± 1.74 mm (22%), respectively (p = .37). Similarly, there were no significant differences in the amount of new cementum formation 4.49 ± 1.56 mm (41%) versus 4.97 ± 1.05 mm (47%), respectively (p = .45).

Conclusions: This experimental study was unable to demonstrate the added value of allogenic cell therapy in supra-crestal periodontal regeneration.
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http://dx.doi.org/10.1111/jcpe.12863DOI Listing
April 2018

Genetic analysis of a rabies virus host shift event reveals within-host viral dynamics in a new host.

Virus Evol 2017 Jul 13;3(2):vex038. Epub 2017 Dec 13.

Wildlife Zoonoses & Vector-Borne Diseases Research Group, Animal and Plant Health Agency, New Haw, Addlestone, Surrey, KT15 3NB, UK.

Host shift events play an important role in epizootics as adaptation to new hosts can profoundly affect the spread of the disease and the measures needed to control it. During the late 1990s, an epizootic in Turkey resulted in a sustained maintenance of rabies virus (RABV) within the fox population. We used Bayesian inferences to investigate whole genome sequences from fox and dog brain tissues from Turkey to demonstrate that the epizootic occurred in 1997 (±1 year). Furthermore, these data indicated that the epizootic was most likely due to a host shift from locally infected domestic dogs, rather than an incursion of a novel fox or dog RABV. No evidence was observed for genetic adaptation to foxes at consensus sequence level and dN/dS analysis suggested purifying selection. Therefore, the deep sequence data were analysed to investigate the sub-viral population during a host shift event. Viral heterogeneity was measured in all RABV samples; viruses from the early period after the host shift exhibited greater sequence variation in comparison to those from the later stage, and to those not involved in the host shift event, possibly indicating a role in establishing transmission within a new host. The transient increase in variation observed in the new host species may represent virus replication within a new environment, perhaps due to increased replication within the CNS, resulting in a larger population of viruses, or due to the lack of host constraints present in the new host reservoir.
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http://dx.doi.org/10.1093/ve/vex038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5729694PMC
July 2017

Corrigendum: Genome Reduction for Niche Association in , A Cause of Spotty Liver Disease in Poultry.

Front Cell Infect Microbiol 2017 14;7:480. Epub 2017 Nov 14.

Bacteriology, Animal and Plant Health Agency Weybridge, Addlestone, United Kingdom.

[This corrects the article on p. 354 in vol. 7, PMID: 28848714.].
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http://dx.doi.org/10.3389/fcimb.2017.00480DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5694627PMC
November 2017

Genome Reduction for Niche Association in , A Cause of Spotty Liver Disease in Poultry.

Front Cell Infect Microbiol 2017 11;7:354. Epub 2017 Aug 11.

Bacteriology, Animal and Plant Health Agency WeybridgeAddlestone, United Kingdom.

The term "spotty liver disease" (SLD) has been used since the late 1990s for a condition seen in the UK and Australia that primarily affects free range laying hens around peak lay, causing acute mortality and a fall in egg production. A novel thermophilic SLD-associated was reported in the United Kingdom (UK) in 2015. Subsequently, similar isolates occurring in Australia were formally described as a new species, . We describe the comparative genomics of 10 isolates recovered from 5 geographically distinct poultry holdings in the UK between 2010 and 2012. Hierarchical gene-by-gene analyses of the study isolates and representatives of 24 known species indicated that is most closely related to the major pathogens and . We observed low levels of within-farm variation, even between isolates collected over almost 3 years. With respect to genome features, we noted that the study isolates had a ~140 Kb reduction in genome size, ~144 fewer genes, and a lower GC content compared to . The most notable reduction was in the subsystem containing genes for iron acquisition and metabolism, supported by reduced growth of in an iron depletion assay. Genome reduction is common among many pathogens and in has likely been driven at least in part by specialization following the occupation of a new niche, the chicken liver.
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http://dx.doi.org/10.3389/fcimb.2017.00354DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5554493PMC
April 2018

Simultaneous lateral bone augmentation and implant placement using a particulated synthetic bone substitute around chronic peri-implant dehiscence defects in dogs.

J Clin Periodontol 2017 Nov 21;44(11):1172-1180. Epub 2017 Sep 21.

Faculty of Odontology, Department of Periodontology, University Complutense of Madrid, Madrid, Spain.

Aim: To determine the histological outcomes when using a highly porous synthetic bone substitute and a cross-linked collagen membrane for lateral bone augmentation of peri-implant dehiscence defects.

Methods: In eight dogs, three treatment groups were randomly allocated at each peri-implant dehiscence defect (mean height × depth = 3 × 1 mm) as follows: (i) synthetic bone substitute covered by a cross-linked collagen membrane (test group), (ii) deproteinized bovine bone mineral covered by a natural collagen membrane (positive control), and (iii) no treatment (negative control). Two healing periods (8 and 16 weeks) were applied.

Results: The differences in healing outcomes between the test and positive control groups were not significant at 8 weeks. Horizontal bone augmentation 2 mm below the implant shoulder was significantly greater in the test group (1.22 ± 0.53 mm) than in the positive and negative controls (0.42 ± 0.51 and 0.36 ± 0.50 mm, respectively) at 16 weeks. Similarly, the augmented tissue thickness at 0, 1, and 2 mm apical to the implant shoulder was significantly greater in the test group than in the positive control group.

Conclusion: The test group showed significantly better histological outcomes for lateral bone augmentation and tissue thickness at 16 weeks compared to both the positive and negative control groups.
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http://dx.doi.org/10.1111/jcpe.12802DOI Listing
November 2017

Hard and soft tissue integration of immediate and delayed implants with a modified coronal macrodesign: Histological, micro-CT and volumetric soft tissue changes from a pre-clinical in vivo study.

J Clin Periodontol 2017 Aug 26;44(8):842-853. Epub 2017 Jul 26.

Section of Periodontology, Faculty of Odontology, University Complutense of Madrid, Madrid, Spain.

Aim: To study the healing of peri-implant tissues around implants with a triangular coronal third (test) or cylindrical (control).

Materials And Methods: In eight beagle dogs, immediate and delayed implants were placed. Test and control implants were randomly assigned and the hard and soft tissue healing was evaluated with histology and micro-CT analysis at 4 and 12 weeks. The soft tissue contour changes were assessed by image analysis software.

Results: When measured at the implant shoulder level, the buccal crestal width (primary outcome assessed in mm) attained similar values in test and control implants. More apically (3 mm) test implants had greater buccal crestal width in delayed and immediate sites. For vertical soft and hard tissue measurements, no significant differences were found between Test and Control. Micro-CT evaluation of the buccal volume of interest showed less volume of implant component in T implants in all sites, although bone volume was not significantly different between T/C. Soft tissue contours were similar around T/C implants.

Conclusion: Triangular implants showed similar percentage of osseointegration, buccal bone volume and soft tissue contours, although attaining greater buccal crestal bone width. No differences were found in regard to soft tissue dimensions and the position of the first bone-to-implant contact.
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http://dx.doi.org/10.1111/jcpe.12747DOI Listing
August 2017