Publications by authors named "Janice Izabel Druzian"

37 Publications

Novel bioactive nanoparticles from crude palm oil and its fractions as foodstuff ingredients.

Food Chem 2022 Mar 9;373(Pt B):131252. Epub 2021 Oct 9.

Center for Agrarian Sciences, Federal University of Santa Catarina, Admar Gonzaga Highway, 1346, Itacorubi, Florianópolis, Santa Catarina 88034-000, Brazil. Electronic address:

Novel bioactive nanoparticles derived from crude palm oil (CPO), palm olein, and palm stearin for use in foodstuff products were produced, and their physicochemical characteristics and stability were evaluated. The nanoparticles were prepared by homogenization, using biodegradable casein or gum arabic as an encapsulating material. The encapsulation efficiency (EE), morphology, long-term stability, particle size, polydispersity index, zeta potential, pH, apparent viscosity, color parameters, total carotenoids, and antioxidant activity were determined. All nanoparticles methods produced spherical nanoparticles with EE higher than 85%. Highly homogeneous small particles (<300 nm) showing a tendency toward a yellow color were observed after 60 days of storage at 4 °C. The nanoparticles showed a carotenoid retention index higher than 40% and an antioxidant activity higher than 1,000 µM Trolox/g oil. The bioactive nanoparticles retained the carotenoids and are proposed as a green innovative product to replace synthetic colorants and antioxidants in foodstuffs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2021.131252DOI Listing
March 2022

Innovative methodological approach using CIELab and dye screening for chemometric classification and HPLC for the confirmation of dyes in cassava flour: A contribution to product quality control.

Food Chem 2021 Dec 24;365:130446. Epub 2021 Jun 24.

Department of Bromatological Analysis, Faculty of Pharmacy, Federal University of Bahia - UFBA, 40170-115 Salvador, Bahia, Brazil; Department of Food Science, School of Nutrition, Federal University of Bahia - UFBA, 40110-907 Salvador, Bahia, Brazil. Electronic address:

A variety of methods for producing cassava flour exist, resulting in very heterogeneous products that exhibit various colours, textures, granulometries, and flavours. To improve its attractiveness to consumers, some producers dye cassava flour with turmeric or tartrazine; however, this practice is illegal in Brazil. In this study, cassava flour samples were collected and evaluated for possible adulteration by the addition of dyes. Flours were analysed by CIELab and dye screening (paper chromatography and the turmeric-identification method) and a classification tree was developed using these data. Positive results for curcuminoid pigments or tartrazine were confirmed by HPLC-DAD or HPLC-UV-Vis, respectively. The developed approach is an innovative alternative chemometric-analysis method that facilitates highly practical screening; adulterated cassava flour, a product of great human-food importance, can be identified using CIELab parameters.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2021.130446DOI Listing
December 2021

Starch chemical modifications applied to drug delivery systems: From fundamentals to FDA-approved raw materials.

Int J Biol Macromol 2021 Aug 16;184:218-234. Epub 2021 Jun 16.

Northeast Biotechnology Network (RENORBIO), Institute of Health Sciences, Federal University of Bahia, Reitor Miguel Calmon, Canela, 40, 231-300 Salvador, BA, Brazil; Faculty of Pharmacy, Federal University of Bahia, Barão de Jeremoabo, 147, Campus Universitário de Ondina, 40, 170-115 Salvador, BA, Brazil.

Starch derivatives are versatile compounds that are widely used in the pharmaceutical industry. This article reviews the advances in the research on hydrophilic and hydrophobic starch derivatives used to develop drug delivery systems over the last ten years, specifically microparticles, nanoparticles, nanocrystals, hydrogels, and scaffolds using these materials. The fundamentals of drug delivery systems, regulatory aspects, and chemical modifications are also discussed, along with the synthesis of starch derivatives via oxidation, etherification, acid hydrolysis, esterification, and cross-linking. The chemical modification of starch as a means to overcome the challenges in obtaining solid dosage forms is also reviewed. In particular, dialdehyde starches are potential derivatives for direct drug attachment; carboxymethyl starches are used for drug encapsulation and release, giving rise to pH-sensitive devices through electrostatic interactions; and starch nanocrystals have high potential as hydrogel fillers to improve mechanical properties and control drug release through hydrophilic interactions. Starch esterification with alginate and acidic drugs could be very useful for site-specific, controlled release. Starch cross-linking with other biopolymers such as xanthan gum is promising for obtaining novel polyelectrolyte hydrogels with improved functional properties. Surface modification of starch nanoparticles by cross-linking and esterification reactions is a potential approach to obtain novel, smart solid dosages.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2021.06.077DOI Listing
August 2021

A Patent Data Analysis in Nanotechnology Applied to Essential Oils.

Recent Pat Nanotechnol 2022 ;16(2):92-106

Faculty of Pharmacy, Federal University of Bahia, Salvador, Brazil.

Background: Essential oils (EOs) are natural substances that serve as sources of bioactive compounds with antioxidant and antimicrobial properties.

Objective: This objective is to understand intellectual property related to patent documents on EOs and nanotechnology.

Methods: The nanotechnology growth curve applied to EOs demonstrated that the period from 2015 to 2017 was the most prominent, with a peak in 2016. China is the dominant country, mainly through research developed in the academic area.

Results: The food industry area had the highest number of patents filed, highlighting the preservation line. Ginger essential oil, chitosan and Tween 80 were preferentially used as a core, wall material, and emulsifier, respectively. In the market, the use of EOs is more associated with the pharmaceutical/ cosmetics industry. In addition, the food industry market bets more on products based on hemp oil nanocapsules.

Conclusion: EO nanotechnology is promising for the development of sustainable food systems. However, this nanotechnology in Brazil has not yet advanced enough in the food industry, although there are government incentives that may change this paradigm in the future. The profile of the patent documents and the products in the market differ between the application and the types of oils. In addition, there is a gap between the volume of patent documents investigated and the transfer of technology to the commercial sector, but this constitution could be better explored, given the properties of EOs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2174/1872210515666210217090541DOI Listing
March 2022

Combination of carotenoids from Spirulina and PLA/PLGA or PHB: New options to obtain bioactive nanoparticles.

Food Chem 2021 Jun 8;346:128742. Epub 2020 Dec 8.

Nutrition School, Federal University of Bahia, Rua Basilio da Gama-s/n-Campus Canela, Salvador, Bahia 40110-907, Brazil; Department of Food Science and Technology, Federal University of Santa Catarina, Admar Gonzaga Highway, 1346, Itacorubi, Florianópolis, Santa Catarina 88034-000, Brazil. Electronic address:

The use of poly-β-hydroxybutyrate (PHB) is an alternative polymer that can be considered environment friendly and renewable to prepare nanoparticles of carotenoids. This study aimed to develop and characterize aqueous dispersion nanoparticles and lyophilized nanoparticles of carotenoid extract obtained from Spirulina sp. LEB 18 by nanoprecipitation, using poly lactic acid (PLA)/poly lactic-co-glycolic acid (PLGA) (75:25 w/w) or PHB as encapsulants. The samples were characterized for the particle size, polydispersity index, zeta potential, apparent viscosity, pH, color parameters, ultraviolet-visible (UV/Vis) spectrophotometry, carotenoid profile, encapsulation efficiency, morphology, and thermal analysis. Nanoparticles containing microalgae carotenoid extract showed average particle diameter on a nanoscale (<200 nm), high homogeneity and stability, high thermal stability, and encapsulation efficiency carotenoid (>80%) when compared to nanoparticles containing β-carotene synthetic. PHB or PLA/PLGA as encapsulating material in the production of nanoparticles from microalgae carotenoids can be a polymeric alternative capable of promoting greater stability and application of carotenoids.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2020.128742DOI Listing
June 2021

Microbial Colorants Production in Stirred-Tank Bioreactor and Their Incorporation in an Alternative Food Packaging Biomaterial.

J Fungi (Basel) 2020 Nov 2;6(4). Epub 2020 Nov 2.

Department of Engineering Bioprocess and Biotechnology, School of Pharmaceutical Sciences, Universidade Estadual Paulista-UNESP, Araraquara 14800-903, Brazil.

Natural colorants from microbial fermentation have gained significant attention in the market to replace the synthetic ones. spp. produce yellow-orange-red colorants, appearing as a potential microorganism to be used for this purpose. In this work, the production of natural colorants by in a stirred-tank bioreactor is studied, followed by its application as additives in bio-based films. The effect of the pH-shift control strategy from 4.5 to 8.0 after 96 h of cultivation is evaluated at 500 rpm, resulting in an improvement of natural colorant production, with this increase being more significant for the orange and red ones, both close to 4-fold. Next, the fermented broth containing the colorants is applied to the preparation of cassava starch-based films in order to incorporate functional activity in biodegradable films for food packaging. The presence of fermented broth did not affect the water activity and total solids of biodegradable films as compared with the standard one. In the end, the films are used to pack butter samples (for 45 days) showing excellent results regarding antioxidant activity. It is demonstrated that the presence of natural colorants is obtained by a biotechnology process, which can provide protection against oxidative action, as well as be a functional food additive in food packing biomaterials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/jof6040264DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712370PMC
November 2020

Supercritical Extraction of Red Propolis: Operational Conditions and Chemical Characterization.

Molecules 2020 Oct 20;25(20). Epub 2020 Oct 20.

School of Pharmacy, Federal University of Bahia (UFBA), Barão de Jeremoabo Street, 147, Salvador 40110-100, Bahia, Brazil.

The objective of this study was to determine the best operational conditions for obtaining red propolis extract with high antioxidant potential through supercritical fluid extraction (SFE) technology, using carbon dioxide (CO) as the supercritical fluid and ethanol as the cosolvent. The following parameters were studied: overall extraction curve, S/F (mass of CO/mass of sample), cosolvent percentage (0, 1, 2 and 4%) and global yield isotherms as a function of different pressures (250, 350 and 450 bar) and temperatures (31.7, 40 and 50 °C). Within the investigated parameters, the best conditions found were an S/F of 131 and the use of ethanol at the highest concentration (4% /), which resulted in higher extract yields and higher content of antioxidant compounds. Formononetin, the main biomarker of red propolis, was the compound found at the highest amounts in the extracts. As expected, the temperature and pressure conditions also influenced the process yield, with 350 bar and 40 °C being the best conditions for obtaining bioactive compounds from a sample of red propolis. The novel results for red propolis found in this study show that it is possible to obtain extracts with high antioxidant potential using a clean technology under the defined conditions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules25204816DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7587948PMC
October 2020

Development of arrowroot flour fermented by kefir grains.

J Food Sci 2020 Nov 29;85(11):3722-3730. Epub 2020 Sep 29.

Biology Department, Microbiology Sector, Federal University of Lavras (UFLA), Lavras, MG, 37200-000, Brazil.

The present study aims to produce arrowroot flour fermented by kefir grains, in addition to assessing the physicochemical, nutritional, and microbiological characteristics. Fermented arrowroot flour was produced at room temperature (approximately 25 to 28 °C). Fermentation was conducted in batch (6 kg of the substrate and 10% of kefir grains were added with homogenization every 3 hr). Samples were evaluated every 12 hr for both fermentation processes (fermentation process 1: 24 hr and fermentation process 2: 48 hr). The flours were evaluated for physicochemical, nutritional, and microbiological qualities, using a completely randomized design, considering only the variation in the duration of both fermentation processes (from 24 to 48 hr). The fermentation process positively modified the physicochemical, nutritional, and microbial characteristics of the flours. An increase in antioxidant activity (IC : control flour [CF] = 18.9 ± 0.13; arrowroot kefir flour [24 hr of fermentation; AKF1] = 15.36 ± 0.14; and arrowroot kefir flour [48 hr of fermentation; AKF2] = 13.84 ± 0.15), protein percentage (CF = 3.08 ± 0.12; AKF1 = 4.87 ± 0.33; and AKF2 = 6.00 ± 0.07), and organic acid (lactic, acetic, and propionic acids) production was observed, as well as modification in color (browning), the conformation of starch structures, and carbohydrate reduction. These results suggested that the "arrowroot kefir flours" open a new perspective for introduction in the market as a new product that can be used as food in nature or food ingredient for making bread, biscuits, pasta, and others, showing microbiological safety and functions properties. PRACTICAL APPLICATION: The fermented flours present improved nutritional characteristics due to the fermentation process, such as higher antioxidant activity and protein levels. Regarding the population growth and societal demand for healthier food, one possibility is to provide a fermented flour with added nutritional value and raise knowledge about the arrowroot. Thus, these flours can be used in various food items or as an ingredient in food preparations for consumers that desire a healthy diet.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/1750-3841.15472DOI Listing
November 2020

Effect of Drying Methods on Bioactive Compounds and Antioxidant Capacity in Grape Skin Residues from the New Hybrid Variety "BRS Magna".

Molecules 2020 Aug 14;25(16). Epub 2020 Aug 14.

Department of Biotechnology, Health Science Institute, Federal University of Bahia (UFBA), Salvador 40170-115, Bahia, Brazil.

The effects of the drying process using the conventional oven and freeze-drying on the thermogravimetric profile, proximate composition, color parameters, individual bioactive compounds, and antioxidant activity in the grape residue (skin) were evaluated. Twenty individual phenolic compounds were identified, where a variation in concentration was observed for flavonols, stilbenes, phenolic acids, flavonoids, procyanidins, and particularly anthocyanins (malvidin-3,5-di--glucoside of 253.2-536.9 mg/kg) due to the drying process. Drying in a conventional oven caused a decrease of 23% of the total polyphenols. The skin of the BRS magna grape has a high concentration of total phenolic content of 489.5-148.3 mg.GAE/100 g, total anthocyanin content of 124.9-260.1 mg.CE/100 g, and total flavonoid content of 12.7-26.0 mg.QE/100 g. The results of free radical scavenging activity (1.26-4.91 μg/mL, as EC) and ferric reducing antioxidant power (82.93-184.10 μmol/g of skin as equivalent to FeSO) indicate high antioxidant activity, independently of the drying process applied. It was concluded that, if the application is directed to anthocyanin compounds, the use of lyophilization is recommended. On the other hand, if the interest is in bioactive compounds that exert antioxidant activity, conventional oven-drying can be used.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules25163701DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466153PMC
August 2020

Preparation and characterization of C-phycocyanin coated with STMP/STPP cross-linked starches from different botanical sources.

Int J Biol Macromol 2020 Sep 18;159:739-750. Epub 2020 May 18.

Faculty of Pharmacy, Federal University of Bahia, Barão de Jeremoabo, 147, Campus Universitário de Ondina, 40.170-115 Salvador, BA, Brazil.

This work aimed to use sodium trimetaphosphate/sodium tripolyphosphate cross-linked potato, banana, corn, cassava, and breadfruit starches as wall materials for C-phycocyanin encapsulation, characterize them and evaluate their in vivo pharmacological effects in an inflammation model. The cross-linked starches were successfully obtained, characterized, and submitted to C-phycocyanin encapsulation by freeze-drying. The characterization of cross-linked starches-C-phycocyanin composites by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetry, and differential scanning calorimetry demonstrated that the C-phycocyanin was encapsulated between amorphous chains of cross-linked starches. Among the five preparations, the cross-linked potato starch presented the highest phosphorous content (0.084%), substitution degree (0.004), water uptake capacity (0.88 g g), and C-phycocyanin encapsulation efficiency (67.58%), thus was tested in vivo. The cross-linked potato starch-C-phycocyanin prolonged the antihyperalgesic effects attributed to C-phycocyanin, evaluated by complete Freund's adjuvant (CFA) model. Starch cross-linking promoted the formation of a hydrogel network in swollen state entrapping C-phycocyanin, thus, acting as a barrier to its release to the medium and promoting long-lasting in vivo effects. The combination of chemical modification of starches followed by physical treatment presented itself as a useful tool for the development of pharmaceutical formulations.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2020.05.111DOI Listing
September 2020

Spirulina sp. LEB 18 cultivation in a raceway-type bioreactor using wastewater from desalination process: Production of carbohydrate-rich biomass.

Bioresour Technol 2020 Sep 7;311:123495. Epub 2020 May 7.

Department of Bromatological Analysis, College of Pharmacy, Federal University of Bahia, Salvador, Brazil.

This study aimed to evaluate the biomass production of Spirulina sp. LEB 18 cultivated in wastewater from the desalination process. The outdoor cultivations (210 L) were performed using as culture medium 100% wastewater supplemented with 25% of Zarrouk constituents (Tcs). In parallel, it was performed a control assay using 100% Zarrouk constituents. The biomass production in Tcs assay (1.14 g L) was only 9% lower than the control assay (1.25 g L). The Tcs assay showed a higher content of carbohydrates (52.29%), lipids (12.79%) and ash (2.69%) compared to the control assay (47.91; 7.59 and 1.29%, respectively). The biomass from the control and Tcs assays had mostly monounsaturated fatty acids C15:1 and C18:2n6t. The Spirulina sp. LEB 18 could use efficiently the nutrients from the wastewater, showing high removal efficiency of NO (96.99%), PO (83.11%) and Z (96.43%). At the same time, high added value biomolecules were produced for different purposes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2020.123495DOI Listing
September 2020

Physicochemical and sensory profile of Beauregard sweet potato beer.

Food Chem 2020 May 24;312:126087. Epub 2019 Dec 24.

Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju, Sergipe CEP 49032-490, Brazil; Tiradentes University (UNIT), Av. Murilo Dantas, 300, Aracaju, Sergipe CEP 49032-490, Brazil.

Beer has been one of the most consumed alcoholic beverages worldwide. However, the incorporation of adjuncts in the beer can add new organoleptic and functional characteristics to the beverage. For this, Beauregard sweet potato shows high potential due to being a rich source of starch and many bioactive compounds. The aim of this study was to develop the best process condition to produce a Sweet potato beer with enhanced nutritional and antioxidant properties and good sensory characteristics. Beer samples showed increased antioxidant activity especially due to β-carotene and their total phenolic content. The phytochemical profile of sweet potato biocompounds demonstrated a direct effect of this adjunct on sensory and functional characteristics of the finished beer. In conclusion, it was found that Beauregard sweet potato is a promising adjunct for beer brewing with nutraceutical properties due to its rich composition of bioactive compounds.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2019.126087DOI Listing
May 2020

Brackish Groundwater from Brazilian Backlands in Spirulina Cultures: Potential of Carbohydrate and Polyunsaturated Fatty Acid Production.

Appl Biochem Biotechnol 2020 Mar 14;190(3):907-917. Epub 2019 Sep 14.

Laboratory of Biochemical Engineering, College of Chemistry and Food Engineering, Federal University of Rio Grande, Rio Grande, RS, Brazil.

The composition of brackish groundwater from Brazilian backlands contains important elements necessary for metabolism in microalgae. This study evaluated the use of 100% brackish groundwater with different amounts of Zarrouk nutrients for Spirulina sp. LEB 18 cultivation. The growth parameters and biomass composition, including the concentrations of proteins, carbohydrates, ash, lipids, and fatty acids, were evaluated. The best growth parameter results were obtained in the assay using 100% brackish groundwater and only 25% of Zarrouk nutrients, which were equal to those obtained for the control culture. The concentrations of carbohydrates and polyunsaturated fatty acids were increased by as much as 4- and 3.3-fold, respectively, when brackish groundwater was used in the cultures. The lipid profile demonstrated that the biomass had the potential for use in biodiesel production. The use of brackish groundwater is a sustainable, economical way to obtain high-quality biomass for different applications during Spirulina sp. LEB 18 cultivation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-019-03126-7DOI Listing
March 2020

Evaluation of the antioxidant profile and cytotoxic activity of red propolis extracts from different regions of northeastern Brazil obtained by conventional and ultrasound-assisted extraction.

PLoS One 2019 5;14(7):e0219063. Epub 2019 Jul 5.

University Center SENAI/CIMATEC, National Service of Industrial Learning - SENAI, Heath Institute of Technology (ITS CIMATEC), Salvador, Bahia, Brazil.

Propolis is a complex mixture of resinous and balsamic material collected from the exudates of plants, shoots, and leaves by bees. This study evaluated red propolis extracts obtained by conventional (ethanolic) extraction and ultrasound-assisted extraction of six samples from different regions of northeastern Brazil. The total phenolic compounds and flavonoids, in vitro antioxidant activity, concentration of formononetin and kaempferol and the cytotoxicity against four human tumor cell lines were determined for all twelve obtained extracts. Significant variations in the levels of the investigated compounds were identified in the red propolis extracts, confirming that the chemical composition varied according to the sampling region. The extraction method used also influenced the resulting propolis compounds. The highest concentration of the compounds of interest and the highest in vitro antioxidant activity were exhibited by the extracts obtained from samples from state of Alagoas. Formononetin and kaempferol were identified in all samples. The highest formononetin concentrations were identified in extracts obtained by ultrasound, thus indicating a greater selectivity for the extraction of this compound by this method. Regarding cytotoxic activity, for the HCT-116 line, all of the extracts showed an inhibition of greater than 90%, whereas for the HL-60 and PC3 lines, the minimum identified was 80%. In general, there was no significant difference (p>0.05) in the antiproliferative potential when comparing the extraction methods. The results showed that the composition of Brazilian red propolis varies significantly depending on the geographical origin and that the method used influences the resulting compounds that are present in propolis. However, regardless of the geographical origin and the extraction method used, all the red propolis samples studied presented great biological potential and high antioxidant activity. Furthermore, the ultrasound-assisted method can be efficiently applied to obtain extracts of red propolis more quickly and with high concentration of biomarkers of interest.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0219063PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6611595PMC
February 2020

Grape peel (Syrah var.) jam as a polyphenol-enriched functional food ingredient.

Food Sci Nutr 2019 May 12;7(5):1584-1594. Epub 2019 Apr 12.

Federal University of Bahia (UFBA) Salvador Brazil.

In this study, we evaluated the effects of the storage time on the physicochemical properties, bioactive compound content, and antioxidant capacity of jam prepared from grape peel extract to explore its potential as a supplementary food and/or functional ingredient. The ethanolic extract from Syrah var. grape peel exhibited high bioactive compound concentrations and antioxidant activity. The jam stability (prepared with 8.9% of extract) at 14°C was evaluated at 0, 15, 30, 45, and 60 days. The jam was found to contain high concentrations of polyphenolic compounds (137.0 ± 3.2 mg of gallic acid equivalent/100 g), total flavonoids (128.5 ± 23.0 mg of equivalent/100 g), and total anthocyanins (92.5 ± 4.0 mg of cyanidin equivalent/100 g). However, a large reduction in the flavonoid (70%-90%), anthocyanin (29%-35%), and phenolic (23%-30%) content was observed during storage. The free radical-scavenging activity (DPPH), ferric reducing antioxidant power (FRAP), and β-carotene-linoleic acid assays revealed the great antioxidant potential of the jam prepared from grape peel extract, which exhibited significant levels of radical-neutralizing activity, especially as determined by the DPPH method with EC values ranging from 2.3 ± 0.1 to 3.9 ± 0.1 µg/ml. High values (> 0.90) were obtained for the correlation between the DPPH results and the concentrations of the compounds of interest. In summary, the high bioactive compound contents and antioxidant capacity of the jam produced from grape peel suggest that it may provide health benefits as a source of natural antioxidants upon incorporation to several food industry products.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/fsn3.981DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6526673PMC
May 2019

Effect of Cellulose Nanocrystals from Different Lignocellulosic Residues to Chitosan/Glycerol Films.

Polymers (Basel) 2019 Apr 10;11(4). Epub 2019 Apr 10.

Department of Food and Beverages, National Service of Industrial Learning, Applied Research Laboratory of Biotechnology and Food, University Center SENAI CIMATEC, Salvador 41650-010, Brazil.

Interest in nanocellulose obtained from natural resources has grown, mainly due to the characteristics that these materials provide when incorporated in biodegradable films as an alternative for the improvement of the properties of nanocomposites. The main purpose of this work was to investigate the effect of the incorporation of nanocellulose obtained from different fibers (corncob, corn husk, coconut shell, and wheat bran) into the chitosan/glycerol films. The nanocellulose were obtained through acid hydrolysis. The properties of the different nanobiocomposites were comparatively evaluated, including their barrier and mechanical properties. The nanocrystals obtained for coconut shell (CS), corn husk (CH), and corncob (CC) presented a length / diameter ratio of 40.18, 40.86, and 32.19, respectively. Wheat bran (WB) was not considered an interesting source of nanocrystals, which may be justified due to the low percentage of cellulose. Significant differences were observed in the properties of the films studied. The water activity varied from 0.601 (WB Film) to 0.658 (CH Film) and the moisture content from 15.13 (CS Film) to 20.86 (WB Film). The highest values for tensile strength were presented for CC (11.43 MPa) and CS (11.38 MPa) films, and this propriety was significantly increased by nanocellulose addition. The results showed that the source of the nanocrystal determined the properties of the chitosan/glycerol films.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/polym11040658DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523815PMC
April 2019

Production and characterization of Spirulina sp. LEB 18 cultured in reused Zarrouk's medium in a raceway-type bioreactor.

Bioresour Technol 2019 Jul 30;284:340-348. Epub 2019 Mar 30.

Bioprospecting and Biotechnology Laboratory, Institute of Biology, Federal University of Bahia, Salvador, Bahia, Brazil.

The objective was to evaluate the effect of reusing Zarrouk's medium on a Spirulina sp. LEB 18 culture by determining kinetic parameters, chemical composition, biofuels, and thermal characterization. Cultivation was performed in a raceway bioreactor for 7 days, the supernatant was reused for four cycles. Culturing the microalga in the reused medium resulted in a cellular yield of 2.30 g L (control) and 2.04, 1.89, 1.73, and 1.15 g L for four cycles with no influence on cell growth and productivity. Biomass with high contents of carbohydrates (58.00%, 3rd cycle), phycocyanin (2.47 mg mL, 1st cycle), and saturated fatty acids (60.13%, 4th cycle) were obtained with an increase in the profiles of C16:0 (45.85%) and C18:2n6 (47.40%) in the 1st cycle. The reuse of Zarrouk's medium allowed obtaining biomass with reduced cost and differentiated characteristics, allowing the exploration of commercially important biomolecules by the completion of up to four cycles.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2019.03.144DOI Listing
July 2019

Microalgae as source of polyhydroxyalkanoates (PHAs) - A review.

Int J Biol Macromol 2019 Jun 16;131:536-547. Epub 2019 Mar 16.

Department of Bromatological Analysis, College of Pharmacy, Federal University of Bahia, Salvador, Bahia, Brazil.

Polyhydroxyalkanoates (PHA) are biopolymers synthesized by different microorganisms and considered substitute powers for petroleum-based plastics because they have similar mechanical properties as synthetic polymers, can be processed in a similar way and are fully biodegradable. Currently commercial PHAs are produced in fermenters using bacteria and large amounts of organic carbon sources and salts in the culture media, accounting for approximately 50% of the total production costs. A greater commercial application of the PHA is limited to a decrease in the cost of production. Several studies suggest that microalgae are a type of microorganisms that can be used to obtain PHAs at a lower cost because they have minimum nutrient requirements for growth and are photoautotrophic in nature, i.e. they use light and CO as their main sources of energy. Thus, this work aims to provide a review on the production of PHAs of different microalgae, focusing on the properties and composition of biopolymers, verifying the potential of using these bioplastics instead of petroleum based plastics. Studies of stimulation PHA synthesis by microalgae are still considered incipient. Still, it is clear that microalgae have the potential to produce biopolymers with lower cost and can play a vital role in the environment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2019.03.099DOI Listing
June 2019

Characterization of amylose and amylopectin fractions separated from potato, banana, corn, and cassava starches.

Int J Biol Macromol 2019 Jul 14;132:32-42. Epub 2019 Mar 14.

Faculty of Pharmacy, Federal University of Bahia, Rua Barão de Jeremoabo, 147, Campus Universitário de Ondina, 40. 170-115, Salvador, BA, Brazil. Electronic address:

Analytical techniques such HPSEC, DSC, and TGA have been employed for amylose determination in starch samples, though spectrophotometry by iodine binding is most commonly used. The vast majority of these techniques require an analytical curve, using amylose and amylopectin standards with physicochemical properties similar to those found in the original starch. The current study aimed to obtain the amylose and amylopectin fractions from potato, banana, corn, and cassava starches, characterize them, and evaluate their behavior via thermogravimetric curves. Blue amylose iodine complex and HPSEC-DRI methods have obtained high purity amylose and amylopectin fractions. All molecular weights of the obtained amylose and amylopectin fractions were similar to those presented in other reports. Different results were obtained by deconvolution of the amylopectin polymodal distribution. All amyloses presented as semi-crystalline V-type polymorphs, while all amylopectin fractions were amorphous. The T of all V presented were directly proportional to their respective crystalline index. TGA evaluations have shown that selective precipitation of amylose with 1-butanol strongly changes its thermal behavior. Therefore, the separation procedure used was an ineffective pathway for obtaining standards for thermal studies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2019.03.086DOI Listing
July 2019

Cocoa's Residual Honey: Physicochemical Characterization and Potential as a Fermentative Substrate by AWRI726.

ScientificWorldJournal 2019 3;2019:5698089. Epub 2019 Feb 3.

Department of Bromatological Analysis, Faculty of Pharmacy, Federal University of Bahia, Barão de Geremoabo Street, s/n, Ondina, 40171-970, Salvador, BA, Brazil.

This study aims to characterize the physicochemical properties of cocoa's residual honey and evaluate its fermentative capacity as a substrate, using AWRI726 as the starter culture for alcoholic fermentation. The research hypothesis was that cocoa's residual honey can be used for the production of fermented beverages. Cocoa's honey has 14.14 g.100 mL of dry material, containing 11.80 g.100 mL of carbohydrates and 1.20% crude protein, in addition to other minor components, such as pectin, lipids, and Fe, Mn, Na, and Zn, with a carbon-to-nitrogen (C/N) ratio (9.8) most suitable for fermentation. Fermentation at 20°C for 240 hours produced a liquid with 16% v/v ethanol (14 g.L in 144 h). However, 24 hours of fermentation produced the maximum ethanol yield (0.373 g.g) and volumetric productivity (0.168 g.L.h), which were associated with a significant increase in the specific cell growth rate. AWR1726 performed satisfactorily in the alcoholic fermentation of cocoa's residual honey, similar to that observed in other fruit beverages, thus suggesting the suitability of cocoa's residual honey for future commercial applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2019/5698089DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6377945PMC
June 2019

Light emitting diodes applied in Synechococcus nidulans cultures: Effect on growth, pigments production and lipid profiles.

Bioresour Technol 2019 May 2;280:511-514. Epub 2019 Feb 2.

Laboratory of Biochemical Engineering, College of Chemistry and Food Engineering, Federal University of Rio Grande, Rio Grande, Brazil. Electronic address:

Researches about light emitting diodes (LEDs) as energy source in microalgae cultivations has been growing in recent years due to its spectral quality, durability and reduced energy consumption. In this study, green, red and yellow LEDs were evaluated as energy source in Synechococcus nidulans LEB 115 cultures. Productivities and specific growth rates were up to 2.5 times greater than in cultures using fluorescent light. The different LED colors evaluated did not influence the chlorophyll, carotenoid or lipid productions. Biomass cultivated with LEDs showed high amounts of saturated fatty acids (above 48%), which is desirable for biodiesel production. In addition to the Synechococcus nidulans LEB 115 growth stimulation, the application of green, red and yellow LEDs in the cultivations produces potential biomass for biodiesel synthesis and other industrial interest biomolecules utilization.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2019.02.009DOI Listing
May 2019

Innovative functional nanodispersion: Combination of carotenoid from Spirulina and yellow passion fruit albedo.

Food Chem 2019 Jul 6;285:397-405. Epub 2019 Feb 6.

Nutrition School, Federal University of Bahia, Rua Basilio da Gama-s/n-Campus Canela, Salvador, Bahia 40110-907, Brazil; Center for Agrarian Sciences, Federal University of Santa Catarina, Admar Gonzaga Highway, 1346, Itacorubi, Florianópolis, Santa Catarina 88034-000, Brazil. Electronic address:

The nanoencapsulation of carotenoids is presented as a positive alternative to broaden the application of these pigments in the food industry. In this study, we investigated the use of yellow passion fruit albedo flour as an encapsulating material for the production of nanodispersions of carotenoid extract obtained from Spirulina sp. LEB 18. Nanodispersions were characterized for their physicochemical properties, antioxidant activity, stability, and retention of carotenoids after 60 days of storage (4 °C). The nanodispersions made from yellow passion fruit albedo flour and microalgae carotenoid extract presented an average particle diameter at the nanometer scale, high antioxidant activity, and carotenoid retention compared to nanodispersions containing synthetic β-carotene. Yellow passion fruit albedo flour as a polymeric material in the production of carotenoid nanodispersions is an alternative to commercial polymers and allows greater stability and broader application of these pigments.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2019.01.181DOI Listing
July 2019

Valorization of crude glycerol based on biological processes for accumulation of lipophilic compounds.

Int J Biol Macromol 2019 May 14;129:728-736. Epub 2019 Feb 14.

Department of Bromatological Analysis, College of Pharmacy, Federal University of Bahia, Salvador, Bahia, Brazil.

Bacteria that are capable of accumulating lipids in their cells as storage compounds can also produce polyhydroxyalkanoates of high technological value, depending on the specific culture conditions. The objective of this study was to utilize crude glycerol from biodiesel (CGB) as a substrate, which is a major byproduct from biodiesel production, to produce lipophilic compounds. Bacillus megaterium INCQS 425 was cultivated and evaluated for the production of lipophilic compounds and the properties of these compounds were investigated. Cultivation of the bacteria in a medium with a C:N ratio of 0.60:1 favored the accumulation of lipids by (17.5%) comprising mainly palmitic acid (13.08%), palmitoleic (39.48%), and especially oleic acid (37.02%), which imparts good characteristics to biodiesel. Meanwhile, cultivation of the bacteria in a medium with a C:N ratio of 4:1 favored the accumulation of polyhydroxyalkanoates (PHA) (3.31gL) mainly comprising medium and long chain PHA. Low crystallinity (<30%) and excellent thermal properties make them suitable for processes that demand high temperatures, such as extrusion. The lipids produced in the present study had satisfactory oxidative stability for the production of quality biodiesel. The polyhydroxyalkanoates produced in the study are of low cost and have promising thermal properties that justify its technological potential, thereby configuring highly competitive bioproducts.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2019.02.077DOI Listing
May 2019

Pilot-scale isolation and characterization of extracellular polymeric substances (EPS) from cell-free medium of Spirulina sp. LEB-18 cultures under outdoor conditions.

Int J Biol Macromol 2019 Mar 3;124:1106-1114. Epub 2018 Dec 3.

College of Pharmacy, Federal University of Bahia, Salvador, Brazil.

This objective of this work was to monitor the EPS production during the growth of Spirulina sp. LEB-18, evaluate the productivity and to characterize the exopolymers obtained on pilot-scale under outdoor conditions. The production of crude EPS occurred in all phases of biomass growth and was approximately ten folds higher than that biomass concentration of Spirulina sp. LEB-18 at the end cultivation, demonstrating the importance of the use of supernatant after harvesting of Spirulina to obtain high value bioproducts. The EPS extracted by Spirulina sp. LEB-18 are typically heteropolymers with one high molecular weight fraction (polysaccharides) with potential to be utilized as an alternative bioflocculant and another fraction of lower molecular mass (proteins). The presence of uronic acids, pyruvates and acyl groups of carbohydrates or carboxylic groups of amino acids in protein moiety is the main responsible for overall negative charge of EPS, which is also of biotechnological importance. Moreover, due to the pseudoplastic behavior of the solutions and high thermal stability, the obtained EPS can be widely applied in several industrial sectors, thus determining its technological and market potentiality.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2018.12.016DOI Listing
March 2019

Influence of nitrogen on growth, biomass composition, production, and properties of polyhydroxyalkanoates (PHAs) by microalgae.

Int J Biol Macromol 2018 Sep 12;116:552-562. Epub 2018 May 12.

Department of Bromatological Analysis, College of Pharmacy, Federal University of Bahia, Salvador, Bahia, Brazil.

This study sought to evaluate influence of nitrogen availability on cell growth, biomass composition, production, and the properties of polyhydroxyalkanoates during cultivation of microalgae Chlorella minutissima, Synechococcus subsalsus, and Spirulina sp. LEB-18. The cellular growth of microalgae reduced with the use of limited nitrogen medium, demonstrating that nitrogen deficiency interferes with the metabolism of microorganisms and the production of biomass. The biochemical composition of microalgae was also altered, which was most notable in the degradation of proteins and chlorophylls and the accumulation of carbonaceous storage molecules such as lipids and polyhydroxyalkanoates. Chlorella minutissima did not produce these polymers even in a nitrogen deficient environment. The largest accumulations of the polyhydroxyalkanoates occurred after a 15 days culture, with a concentration of 16% (dry cell weight) produced by the Synechococcus subsalsus strain and 12% by Spirulina sp. LEB-18. Polyhydroxyalkanoates produced by Synechococcus subsalsus and Spirulina sp. LEB-18 presented different thermal and physical properties, indicating the influence of producing strain on polyhydroxyalkanoates properties. The polymers obtained consisted of long chain monomers with 14 to 18 carbon atoms. This composition is novel, as it has not previously been found in PHAs obtained from Synechococcus subsalsus and Spirulina sp. LEB-18.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2018.05.064DOI Listing
September 2018

Validation of analytical method for rhynchophorol quantification and stability in inorganic matrix for the controlled release of this pheromone.

Chem Cent J 2018 May 10;12(1):54. Epub 2018 May 10.

Faculty of Pharmacy/RENORBIO, Federal University of Bahia, Rua Barão de Jeremoabo, 147, Campus Universitário de Ondina, Salvador, BA, 40170-115, Brazil.

A fast method for the identification and stability evaluation of the aggregation pheromone rhynchophorol, which is the main substance used for chemical communication by the beetle Rhynchophorus palmarum L., was validated. In addition, the technique was applied to the evaluation of two inorganic matrices, with the objective of using them as controlled-release devices. The analytical method showed good linearity (R = 0.9978), precision (CV% < 1.79), recovery (84-105%) and limits of detection (0.2 mg mL) and quantification (0.3 mg mL); in compliance with the validation legislation established by ANVISA. In the interaction study, the inorganic matrices zeolite L and Na-magadiite showed high rates of pheromone recovery without promoting its degradation for a period of 180 days, which is not reported in the literature for other matrices. The structures of the zeolite L/rhynchophorol and Na-magadiite/rhynchophorol composites showed slower release kinetics during the storage period when compared with pure pheromone, which is desirable since it extends the period of rhynchophorol release and decreases the negative effects caused by the environmental parameters.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13065-018-0426-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5945565PMC
May 2018

Xanthan Gum Production by Xanthomonas campestris pv. campestris IBSBF 1866 and 1867 from Lignocellulosic Agroindustrial Wastes.

Appl Biochem Biotechnol 2018 Nov 5;186(3):750-763. Epub 2018 May 5.

Department of Chemical Engineering, Federal University of Bahia, Aristides Novis, N°2, Federação, Salvador, BA, 40210-360, Brazil.

This study aimed to evaluate the properties of xanthan gum produced by Xanthomonas campestris pv. campestris 1866 and 1867 from lignocellulosic agroindustrial wastes. XG was produced using an orbital shaker in a culture medium containing coconut shell (CS), cocoa husks (CH), or sucrose (S) minimally supplemented with urea and potassium. The XG production results varied between the CS, CH, and S means, and it was higher with the CH in strains 1866 (4.48 g L) and 1867 (3.89 g L). However, there was more apparent viscosity in the S gum (181.88 mPas) and the CS gum (112.06 mPas) for both 1866 and 1867, respectively. The ability of XG and XG to emulsify different vegetable oils was similar to the ability of XG. All gums exhibited good thermal stability and marked groups in the elucidation of compounds and particles with rough surfaces.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-018-2765-8DOI Listing
November 2018

Investigation of cellular fatty acid composition of Xanthomonas spp. as chemical markers of productivity and quality of xanthan gum.

Carbohydr Polym 2018 Jul 23;192:291-298. Epub 2018 Mar 23.

Department of Bromatological Analysis, College of Pharmacy, Federal University of Bahia, Salvador, Bahia, Brazil. Electronic address:

In this study, we investigated the cellular fatty acid profiles of different Xanthomonas pathovars producing xanthan gum and explored the fatty acid composition to identify chemical markers of xanthan gum productivity and quality. Three Xanthomonas pathovars were studied. The fermentation was conducted for 168 h. Samples from the fermented medium were collected for extraction, quantification, and characterization of xanthan. The unsaturated/saturated (U/S) fatty acid ratio in Xanthomonas cells during fermentation was correlated with production, viscosity, and molecular weight of the gum obtained at each 24 h. The Xanthomonas axonopodis pv manihotis 290 strain showed a higher U/S ratio for major cell fatty acids (C16:1ω7/C16:0) as compared with the other two strains; this high ratio was directly associated with xanthan production. No correlation was observed between cellular fatty acid composition and characteristics of xanthan synthesized. Thus, it was possible to determine a production chemical marker for xanthan gum in Xanthomonas strains.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.carbpol.2018.03.078DOI Listing
July 2018

In vitro and in silico studies of 3-hydroxy-3-methyl-glutaryl coenzyme A reductase inhibitory activity of the cowpea Gln-Asp-Phe peptide.

Food Chem 2018 Sep 29;259:270-277. Epub 2018 Mar 29.

School of Pharmacy, Federal University of Bahia, Barão de Jeremoabo Street, 40170-115 Salvador, BA, Brazil. Electronic address:

Previous studies have shown that cowpea protein positively interferes with cholesterol metabolism. In this study, we evaluated the ability of the fraction containing peptides of <3 kDa, as well as that of the Gln-Asp-Phe (QDF) peptide, derived from cowpea β-vignin protein, to inhibit HMG-CoA reductase activity. We established isolation and chromatography procedures to effectively obtain the protein with a purity above 95%. In silico predictions were performed to identify peptide sequences capable of interacting with HMG-CoA reductase. In vitro experiments showed that the fraction containing peptides of <3 kDa displayed inhibition of HMG-CoA reductase activity. The tripeptide QDF inhibits HMG-CoA reductase (IC = 12.8 μM) in a dose-dependent manner. Furthermore, in silico studies revealed the binding profile of the QDF peptide and hinted at the molecular interactions that are responsible for its activity. Therefore, this study shows, for the first time, a peptide from cowpea β-vignin protein that inhibits HMG-CoA reductase and the chemical modifications that should be investigated to evaluate its binding profile.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2018.03.132DOI Listing
September 2018

Outdoor pilot-scale cultivation of Spirulina sp. LEB-18 in different geographic locations for evaluating its growth and chemical composition.

Bioresour Technol 2018 May 9;256:86-94. Epub 2018 Feb 9.

Department of Bromatological Analysis, College of Pharmacy, Federal University of Bahia, Salvador, Brazil.

This study evaluated whether outdoor cultivation of Spirulina sp. in different geographical locations affected its growth and biomass quality, with respect to the chemical composition, volatile compound and heavy metal content, and thermal stability. The positive effect of solar radiation and temperature on biomass productivity in Spirulina sp. cultivated in the northeast was directly related to its improved nutritional characteristics, which occurred with an increase in protein, phycocyanin, and polyunsaturated fatty acid (mainly γ-linolenic) content. The biomass produced in Northeast and South Brazil showed high thermal stability and had volatile compounds that could be used as biomarkers of Spirulina, and their parameters were within the limits of internationally recognized standards for food additives; hence, they have been considered safe foods. However, the growth of crops in south Brazil occurred at lower rates due to low temperatures and luminous intensities, indicative of the robustness of microalgae in relation to these parameters.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2018.01.149DOI Listing
May 2018
-->