Publications by authors named "Hui Zhou"

1,561 Publications

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Semiconducting polymer nano-radiopharmaceutical for combined radio-photothermal therapy of pancreatic tumor.

J Nanobiotechnology 2021 Oct 24;19(1):337. Epub 2021 Oct 24.

State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection & School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, 215123, Jiangsu, China.

Background: Pancreatic ductal adenocarcinoma (PDAC) is a devastatingly malignant tumor with a high mortality. However, current strategies to treat PDAC generally have low efficacy and high side-effects, therefore, effective treatment against PDAC remains an urgent need.

Results: We report a semiconducting polymer nano-radiopharmaceutical with intrinsic photothermal capability and labeling with therapeutic radioisotope Lu (Lu-SPN-GIP) for combined radio- and photothermal therapy of pancreatic tumor. Lu-SPN-GIP endowed good stability at physiological conditions, high cell uptake, and long retention time in tumor site. By virtue of combined radiotherapy (RT) and photothermal therapy (PTT), Lu-SPN-GIP exhibited enhanced therapeutic capability to kill cancer cells and xenograft tumor in living mice compared with RT or PTT alone. More importantly, Lu-SPN-GIP could suppress the growth of the tumor stem cells and reverse epithelial mesenchymal transition (EMT), which may greatly reduce the occurrence of metastasis.

Conclusion: Such strategy we developed could improve therapeutic outcomes over traditional RT as it is able to ablate tumor with relatively lower doses of radiopharmaceuticals to reduce its side effects.
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http://dx.doi.org/10.1186/s12951-021-01083-0DOI Listing
October 2021

Effect of Rosin Modification on the Visual Characteristics of Round Bamboo Culm.

Polymers (Basel) 2021 Oct 12;13(20). Epub 2021 Oct 12.

Department of Biomaterials, International Center for Bamboo and Rattan, Beijing 100102, China.

Rosin was used to treat round bamboo culm using the impregnation method. The quantitative color and gloss measurements combined with a qualitative eye tracking experiment were used to evaluate the effect of rosin treatment under different temperatures on the visual characteristics of the bamboo surface. Surface morphology analysis was also used to explore the mechanism of modification. The results showed that proper heating of the modified system was conducive to the formation of a continuous rosin film, which increased the gloss value. The maximum gloss value of 19.6 achieved at 50 °C was 122.7% higher than the gloss value of the control group. Heating decreased the brightness of the bamboo culm and changed the color from the green and yellow tones to red and blue. Additionally, at temperatures higher than 60 °C, the bamboo epidermal layer was damaged or shed, and stripes formed on the culm surface. The density of these stripes increased with an increase in treatment temperature. Eye movement experiment and subjective evaluation showed that high gloss would produce dazzling feeling, such as at 50 °C, while low gloss will appear dim, such as at 80 °C, while the gloss at 40 °C and 60 °C were appropriate. Additionally, the solid color surface below 60 °C had a large audience of about 73%, and the striped surface above 60 °C was preferred by 27% of the subjects.
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http://dx.doi.org/10.3390/polym13203500DOI Listing
October 2021

A 3D-2D Multibranch Feature Fusion and Dense Attention Network for Hyperspectral Image Classification.

Micromachines (Basel) 2021 Oct 18;12(10). Epub 2021 Oct 18.

Key Laboratory of Water Big Data Technology of Ministry of Water Resources, Hohai University, No. 8 Focheng Road, Nanjing 211100, China.

In recent years, hyperspectral image classification (HSI) has attracted considerable attention. Various methods based on convolution neural networks have achieved outstanding classification results. However, most of them exited the defects of underutilization of spectral-spatial features, redundant information, and convergence difficulty. To address these problems, a novel 3D-2D multibranch feature fusion and dense attention network are proposed for HSI classification. Specifically, the 3D multibranch feature fusion module integrates multiple receptive fields in spatial and spectral dimensions to obtain shallow features. Then, a 2D densely connected attention module consists of densely connected layers and spatial-channel attention block. The former is used to alleviate the gradient vanishing and enhance the feature reuse during the training process. The latter emphasizes meaningful features and suppresses the interfering information along the two principal dimensions: channel and spatial axes. The experimental results on four benchmark hyperspectral images datasets demonstrate that the model can effectively improve the classification performance with great robustness.
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http://dx.doi.org/10.3390/mi12101271DOI Listing
October 2021

Therapeutic Interaction of Apatinib and Chidamide in T-Cell Acute Lymphoblastic Leukemia through Interference with Mitochondria Associated Biogenesis and Intrinsic Apoptosis.

J Pers Med 2021 Sep 29;11(10). Epub 2021 Sep 29.

Department of Hematology, The First Affiliated Hospital of Xiamen University and Institute of Hematology, School of Medicine, Xiamen University, Xiamen 361003, China.

T-cell acute lymphoblastic leukemia (T-ALL) shows poor clinical outcome and has limited therapeutic options, indicating that new treatment approaches for this disease are urgently required. Our previous study demonstrated that apatinib, an orally selective VEGFR-2 antagonist, is highly effective in T-ALL. Additionally, chidamide, a histone deacetylase inhibitor, has proven to be cytotoxic against T-ALL in preclinical and clinical settings. However, whether the therapeutic interaction of apatinib and chidamide in T-ALL remains unknown. In this study, apatinib and chidamide acted additively to decrease cell viability and induce apoptosis in T-ALL in vitro. Notably, compared with apatinib or chidamide alone, the combinational regimen was more efficient in abrogating the leukemia burden in the spleen and bone marrow of T-ALL patient-derived xenograft (PDX) models. Mechanistically, the additive antileukemia effect of apatinib and chidamide was associated with suppression of mitochondrial respiration and downregulation of the abundance levels of several rate-limiting enzymes that are involved in the citric acid cycle and oxidative phosphorylation (OXPHOS). In addition, apatinib enhanced the antileukemia effect of chidamide on T-ALL via activation of the mitochondria-mediated apoptosis pathway and impediment of mitochondrial biogenesis. Taken together, the study provides a potential role for apatinib in combination with chidamide in the management of T-ALL and warrants further clinical evaluations of this combination in patients with T-ALL.
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http://dx.doi.org/10.3390/jpm11100977DOI Listing
September 2021

Valorization of Polysaccharides Obtained from Dark Tea: Preparation, Physicochemical, Antioxidant, and Hypoglycemic Properties.

Foods 2021 Sep 26;10(10). Epub 2021 Sep 26.

Development Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, 100 Guilin Road, Xuhui District, Shanghai 200234, China.

The structure and hypoglycemic activity of tea polysaccharides has been extensively studied, while there are few reports on the characterization and hypoglycemic activity of dark tea polysaccharides. The crude dark tea polysaccharide (CDTPS) was optimally extracted from Fuzhuan dark tea. Six polysaccharide fractions (namely DTPS-1, DTPS-2, DTPS-3, DTPS-4, DTPS-5, and DTPS-6) were isolated from CDTPS, and their physicochemical, structural, and biological properties were compared and analyzed. The results revealed that the compositions, structural characteristics, and biological properties of the six DTPSs were different. Therein, DTPS-4 and DTPS-6 had looser morphology, faster solubility, and a more stable structure. Additionally, DTPS-4 had the optimum in vitro antioxidant capabilities, and DTPS-6 had the strongest in vitro hypoglycemic capabilities. In addition, a correlation analysis revealed that the molecular weight and uronic acid content were significantly related to their antioxidant and hypoglycemic activities. Our results indicated that DTPS-4 and DTPS-6 could be further developed into functional foods or additives, respectively.
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http://dx.doi.org/10.3390/foods10102276DOI Listing
September 2021

Why are Individuals with Alexithymia Symptoms More Likely to Have Mobile Phone Addiction? The Multiple Mediating Roles of Social Interaction Anxiousness and Boredom Proneness.

Psychol Res Behav Manag 2021 8;14:1631-1641. Epub 2021 Oct 8.

Psychological Counseling Center of Student Affairs Office, Zhangzhou City College, Zhangzhou, People's Republic of China.

Purpose: Previous studies have investigated the relationship between alexithymia and problematic mobile phone use (PMPU). However, yet gaps in identifying the internal mechanisms of this relationship remain. Hence, based on the Interaction of Person-Affect-Cognition-Execution model, the current research examined the mediating roles of college students' social interaction anxiousness (SIA) and boredom proneness (BPS) in the relationship between alexithymia and PMPU.

Methods: A total of 1300 college students ( = 20.36 years, = 0.97) were recruited from two universities in the southeast of China to complete a series of questionnaires, including the Toronto alexithymia scale, SIA scale, BPS scale, and mobile phone addiction index questionnaire. In order to conduct conservative predictions, the demographic variables (ie, gender) were controlled as covariates.

Results: The results of multiple mediation analysis showed that (1) alexithymia was positively linked with PMPU; (2) both SIA and BPS mediated the link between alexithymia and PMPU; and (3) a serial indirect pathway emerged (ie, alexithymia → SIA → BPS → PMPU).

Conclusion: These findings indicated that alexithymia could influence PMPU in a simple indirect way (parallel mediation) and in a complex indirect way (serial mediation). Besides, these findings provide some insights into the prevention and intervention of PMPU.
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http://dx.doi.org/10.2147/PRBM.S328768DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8518138PMC
October 2021

Assessment of TMB, PD-L1, and lymphocyte to monocyte ratio as predictive potential in a phase Ib study of sintilimab in patients with advanced solid tumors.

Am J Cancer Res 2021 15;11(9):4259-4276. Epub 2021 Sep 15.

The First Affiliated Hospital, Zhejiang University School of Medicine Hangzhou, Zhejiang, China.

Background: Sintilimab is a humanized monoclonal antibody against the programmed cell death 1 (PD-L1). We aimed to assess the safety and activity of sintilimab monotherapy or in combination with chemotherapy in advanced solid tumors.

Methods: This phase Ib study included six cohorts. Cohort A-C were sintilimab monotherapy settings, and enrolled pretreated patients (2/3 L cohorts). Cohort D-F were treatment-naïve patients (1 L cohorts), and received sintilimab plus different chemotherapies. The primary endpoints were safety and objective response rate (ORR). Exploratory endpoints were potential biomarkers for the prognosis after treatment, such as tumor mutation burden scores (TMB), PD-L1 and lymphocyte-to-monocyte ratio (LMR).

Results: The ORR was 14.6% in the 2/3 L cohorts (n=146), and 73.2% in the 1 L cohorts (n=61). The incidence of grade 3-4 adverse events occurred in 55 patients (37.7%) in 2/3 L cohorts, and in 38 (62.3%) in 1 L cohorts. 157 patients had available TMB scores, and in 2/3 L cohorts, patients in the high TMB groups (TMB≥10) showed a longer progression-free survival (PFS) and overall survival (OS) than those in the low TMB groups (TMB<10). No significant differences in PFS and OS were observed across different PD-L1 groups in both 1 L and 2/3 L cohorts. A high LMR was significantly associated with an improved PFS in 1 L cohorts (P=0.022).

Conclusion: Sintilimab alone or combined with chemotherapy had a tolerable safety profile in solid tumors. The combination therapy showed a favorable activity with advanced non-small cell lung cancer and gastric or esophagogastric junction adenocarcinoma. LMR might be a prognostic factor for the combination regimen in these patients.

Trial Registration: ClinicalTrials.gov, number NCT02937116. Registered 18 October 2016.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8493392PMC
September 2021

Inhibition of progesterone receptor membrane component-1 exacerbates neonatal hypoxic-ischemic cerebral damage in male mice.

Exp Neurol 2021 Oct 12;347:113893. Epub 2021 Oct 12.

Department of Neurology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, China; Institute of Neurological Diseases, Xuzhou Medical University, Xuzhou, China. Electronic address:

This study investigated the expression of progesterone receptor membrane component 1 (pgrmc1) in the brains of male and female mice, and the effect of inhibiting pgrmc1 on neonatal hypoxic-ischemic (HI) cerebral injury in male mice. A mouse model of neonatal HI brain injury was established, and AG205, a specific antagonist of pgrmc1, was injected into the left lateral cerebral ventricle 1 h before HI. Histological staining, behavior testing, Western blots, and quantitative PCR (qPCR) were employed to evaluate pgrmc1 expression, brain damage, neurological function, and molecular mechanisms. Results demonstrated that the mRNA and protein levels of pgrmc1 increased significantly in the cortex and hippocampus 72 h after HI without sex differences. The inhibition of pgrmc1 exacerbated the neonatal brain damage in the acute stage of HI in male mice as seen in the increase in brain water content, infarction area, and neuronal death. Inhibition of pgrmc1 also aggravated the neurological dysfunction and anxiety induced by HI brain injury. In addition, inhibition of pgrmc1 activated the NF-kB signaling and NF-κB-mediated cytokines, and inhibited BDNF/PI3K/AKT pathway in the brains of the newborn HI mice. The results indicated that pgrmc1 inhibition exacerbated the brain damage in newborn male mice subjected to HI by activating IκBα/NFκB signaling and inhibiting BDNF/PI3K/Akt pathway.
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http://dx.doi.org/10.1016/j.expneurol.2021.113893DOI Listing
October 2021

Analysis of Microbial Diversity and Dynamics During Bacon Storage Inoculated With Potential Spoilage Bacteria by High-Throughput Sequencing.

Front Microbiol 2021 28;12:713513. Epub 2021 Sep 28.

College of Food Science and Light Industry, Nanjing Tech University, Nanjing, China.

, , and were investigated for their roles in in the spoilage of sterilized smoked bacon. These five strains, individually and in combination, were applied as starters on sliced bacon at 4-5 log CFU/g using a hand-operated spraying bottle and stored for 45 days at 0-4°C. Dynamics, diversity, and succession of microbial community during storage of samples were studied by high-throughput sequencing (HTS) of the V3-V4 region of the 16S rRNA gene. A total of 367 bacterial genera belonging to 21 phyla were identified. Bacterial counts in all the inoculated specimens increased significantly within the first 15 days while the microbiota developed into more similar communities with increasing storage time. At the end of the storage time, the highest abundance of (96.46%) was found in samples inoculated with . Similarly, for samples inoculated with and , a sharp increase in and abundance was observed as they reached a maximum relative abundance of 97.95 and 81.6%, respectively. Hence, these species were not only the predominant ones but could also have been the more competitive ones, potentially inhibiting the growth of other microorganisms. By analyzing the bacterial load of meat products using the SSO model, the relationships between the microbial communities involved in spoilage can be understood to assist further research.
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http://dx.doi.org/10.3389/fmicb.2021.713513DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8506151PMC
September 2021

Long non-coding ribonucleic acid H19 and ten-eleven translocation enzyme 1 messenger RNA expression levels in uterine fibroids may predict their postoperative recurrence.

Clinics (Sao Paulo) 2021 11;76:e2671. Epub 2021 Oct 11.

Department of Obstetrics and Gynecology, The People's Hospital of Pingyi County, Linyi, Shandong, China.

Objectives: To investigate the predictive value of long non-coding RNA (lncRNA) H19 and the ten-eleven translocation enzyme 1 (TET1) transcriptional expression in postoperative recurrence of uterine fibroids (UFs).

Methods: Seventy-five patients with UF, who underwent surgical treatment, were enrolled in the treatment group, and 60 healthy individuals were enrolled in the control group. The relative expression levels of lncRNA H19 and TET1 mRNA in the serum and UF tissues were analyzed. The patients were further divided into a better curative (BC) group and a poor efficacy (PE) group to analyze the predictive value of lncRNA H19 and TET1 and the independent risk factors affecting the recurrence of UF.

Results: Compared with the control group, lncRNA H19 expression levels were significantly higher, while TET1 expression levels were significantly lower in the treatment group (p<0.001). The area under the receiver operating characteristic (ROC) curve (AUC) values of the two indicators for diagnostic importance were found to be 0.872 and 0.826, respectively. Compared with the PE group, lncRNA H19 expression levels were significantly lower, while TET1 expression levels were significantly higher in the BC group (p<0.001). The AUC values of the two indicators for their predictive efficacy were 0.788 and 0.812, respectively. Logistic regression analysis showed that age, menarche age, maximum diameter of UFs, number of UFs, lncRNA H19 levels, and TET1 levels were independent risk factors affecting UF recurrence. The AUC values of lncRNA H19 and TET1 for their predictive value for postoperative recurrence were 0.814 and 0.765, respectively.

Conclusions: The lncRNA H19 and TET1 have high diagnostic and predictive efficacy for determining the postoperative recurrence of UFs.
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http://dx.doi.org/10.6061/clinics/2021/e2671DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8478142PMC
October 2021

A Functionalized Octahedral Palladium Nanozyme as a Radical Scavenger for Ameliorating Alzheimer's Disease.

ACS Appl Mater Interfaces 2021 Oct 12. Epub 2021 Oct 12.

Department of Chemistry, College of Chemistry and Materials Science, Jinan University, Guangzhou 510632, P. R. China.

Oxidative stress is always mentioned as a pathologic appearance of Alzheimer's disease (AD). It is attributed to mitochondrial dysfunction closely linked to Aβ deposition and neurofibrillary tangles (NFTs). Octahedral palladium nanoparticles (Pd NPs) exhibited excellent antioxidant enzyme-like activity and outstanding biocompatibility, but the poor blood-brain barrier (BBB) permeability limits their application in the treatment of Alzheimer's disease. Herein, we constructed a borneol (Bor)-modified octahedral palladium ([email protected]@Bor) nanozyme platform to eliminate intracellular reactive oxygen species (ROS) and elevate epithelial cell penetrability. Based on in vitro and in vivo studies, we demonstrate that the [email protected]@Bor could efficiently reduce ROS and Ca contents, maintain the mitochondrial membrane potential, and further protect the mitochondria in SH-SY5Y cells. Furthermore, the nanozymes could quickly accumulate in the brain of AD mice and alleviate pathological characteristics such as Aβ plaque deposition, neuron loss, and neuroinflammation. The learning ability and memory function of AD mice are also significantly improved. Overall, this work indicates that the [email protected]@Bor nanozymes could delay the progression of AD by regulating ROS levels and also provides a new strategy for the treatment of AD.
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http://dx.doi.org/10.1021/acsami.1c06687DOI Listing
October 2021

Effect of Molecular Weight and Degree of Substitution on the Physical-Chemical Properties of Methylcellulose-Starch Nanocrystal Nanocomposite Films.

Polymers (Basel) 2021 Sep 27;13(19). Epub 2021 Sep 27.

School of Food Science and Technology, Hunan Agricultural University, 410128 Hunan, China.

This research studied the effect of molecular weight (M) and degree of substitution (DS) on the microstructure and physicochemical characteristics of methylcellulose (MC) films with or without SNC. The M and DS of three types of commercial MC (trade name of M20, A4C, and A4M, respectively) were in the range of 0.826 to 3.404 × 10 Da and 1.70 to 1.83, respectively. M significantly affected the viscosity of methylcellulose solutions as well as the microstructure and tensile strength of methylcellulose films, while DS had a pronounced effect on their oxygen permeability properties. The incorporation of 15% () SNC resulted in the efficient improvement of tensile strength, water, and oxygen barrier properties of films, particularly for the A4C nanocomposite films. The results from SEM and FTIR illustrated that relatively homogenous dispersion of SNC was distinguished in A4C-15% () SNC films. Furthermore, microstructures of MC-SNC nanocomposite films were strongly dependent on both M and DS of MC. This work offers a convenient and green method to fabricate MC-based nanocomposite films with desirable mechanical, light, oxygen, and water vapor barrier properties.
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http://dx.doi.org/10.3390/polym13193291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8513015PMC
September 2021

Feasibility of ultrasound-induced blood-brain barrier disruption with a single-element transducer under three different frequencies in two non-human primates in vivo: Case report.

J Neurosci Methods 2021 Oct 8;365:109383. Epub 2021 Oct 8.

Paul C. Lauterbur Research Center for Biomedical Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China. Electronic address:

Background: Single-element focused transducers applied in blood-brain barrier (BBB) disruption experiments to optimize intravascular therapies in CNS diseases have the advantage of low cost and portability. Most of the in vivo studies on non-human primates report the use of single-element transducers with an annular spherical shape and a central frequency of 500 kHz. High-frequency ultrasound has smaller focal area and less standing-wave effect but lower transcranial penetration efficiency. Our study reports the feasibility and safety concerns of BBB opening by single-element spherical transducers with central frequencies of 300, 650 and 800 kHz on two rhesus macaques.

Methods: Pulsed ultrasound exposure (3-minute duration, 0.5-1% duty cycle) combined with microbubble injection (SonoVue, 0.2uL/g) was used to disrupt the BBB of the monkeys under the magnetic resonance imaging (MRI) guidance. Gadolinium contrast-enhanced MRI was used to confirm and evaluate the BBB opening after sonication. T2-weighted fast spin echo and T2 * -weighted gradient echo sequences were used to check the post-sonication complications, such as edema and micro-bleeding.

Results: Contrast enhancement was found on the post-sonication T1 weighted images for all experiments, showing that the BBB was successfully opened under all the three frequencies on both monkeys. The enhanced area was largest at the lowest frequency. No obvious hypo-intensity or hyper-intensity was observed on either the T2 * weighted gradient echo images or T2-weighted fast-spin echo images, implying the safety of the opening procedure. However, signal enhancement was also observed in the subarachnoid space of the sulci for all frequencies, indicating that the BBB was also disrupted in the propagation path outside the focal area.

Conclusions: The feasibility of BBB opening with single-element transducer under frequencies ranging from 300 kHz to 800 kHz was confirmed by experiments in two non-human primates in vivo. Further investigation into the off-target effects and transducer configurations is needed for safety optimization.
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http://dx.doi.org/10.1016/j.jneumeth.2021.109383DOI Listing
October 2021

[Clinical application of nasal mucosa flap in the repair of nasopharyngeal skull base bone exposure after radiotherapy for nasopharyngeal carcinoma].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2021 Oct;35(10):929-934

Department of Otorhinolaryngology Head and Neck Surgery,Affiliated Hospital of Guangdong Medical University,Guangzhou,524001,China.

To observe the effect of nasal mucosa flap in the repair of nasopharyngeal skull base bone exposure after radiotherapy for nasopharyngeal carcinoma, and to provide a basis for the repair with nasal mucosa flap in skull base bone exposure after radiotherapy. The clinical data of 8 patients who underwent nasal endoscopic surgery were analyzed retrospectively. The survival of mucosal flap, the mucosal epithelialization of bone defect or exposed site, the improvement of main clinical symptoms and complications were followed up after operation. Severe mucosal flap necrosis and bone exposure occurred in 1 case after operation, in the other 7 cases, the mucosal flap survived and the mucosal epithelium of nasopharynx recovered well. After operation, most of the patients' clinical symptoms such as headache and nasal odor were improved compared with those before operation. Nasal mucosal flap is a safe and minimally invasive autogenous material with good biocompatibility. It has a good application prospect in repairing bone defect or exposure of nasopharyngeal skull base after radiotherapy and is worth popularizing in clinic.
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http://dx.doi.org/10.13201/j.issn.2096-7993.2021.10.014DOI Listing
October 2021

Manufacturable low-crosstalk high-RCMF 13-core 5-LP mode fiber with graded-index core and stairway-index trench.

Opt Express 2021 Aug;29(17):26418-26432

We propose a novel scheme of high doped core and stairway-index trench structure to design a manufacturable graded-index 13-core 5-LP mode fiber with low inter-core crosstalk (ICXT) and large mode differential group delay (MDGD). By using the couple power theory and the finite element method (FEM), the change ICXT of with fiber parameters are investigated. The design of core graded profile and trench structure are optimized to achieve better performance and to meet the fabrication conditions. The numerical result demonstrate that this fiber achieves a low ICXT of lower than -30dB/km (Rb≤500 mm). The bending loss values satisfy the ITU-T recommendation G.655 in 195 µm cladding diameter. Furthermore, the dispersion and the MDGD dependences on wavelength are calculated. The relative core multiplicity factor (RCMF) is obtained as 75.17, which realizes the high density multiplexing. The fabrication methods of this fiber are briefly introduced. The designed fiber may be used for Space-division multiplexing (SDM) system to solve the problem of fiber capacity limitation.
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http://dx.doi.org/10.1364/OE.434654DOI Listing
August 2021

A zinc/PyBisulidine catalyzed asymmetric Mannich reaction of N-tosyl imines with 3-acyloxy-2-oxindoles.

Org Biomol Chem 2021 Sep 19;19(34):7460-7469. Epub 2021 Aug 19.

Chongqing Research Center for Pharmaceutical Engineering, College of Pharmacy, Chongqing Medical University, Chongqing 400016, China.

A Zn-PyBisulidine catalyzed asymmetric Mannich reaction of 3-acyloxy-2-oxindoles has been developed. Various quaternary substituted 3-acyloxy-2-oxindoles bearing vicinal amino alcohol motifs were obtained in good to excellent yields with moderate to excellent dr and excellent enantioselectivities. The utility of this reaction was demonstrated by the easy removal of the acyl group to give C3-hydroxy derivatives and their application as a key skeleton of the ligand for the Ni-catalyzed enantioselective Henry reaction.
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http://dx.doi.org/10.1039/d1ob01328aDOI Listing
September 2021

An elevated deoxycholic acid level induced by high-fat feeding damages intestinal stem cells by reducing the ileal IL-22.

Biochem Biophys Res Commun 2021 Nov 25;579:153-160. Epub 2021 Sep 25.

Department of Gastroenterology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai Key Laboratory of Pancreatic Diseases, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of Gastroenterology, Shanghai General Hospital, Nanjing Medical University, Nanjing, China. Electronic address:

Long-term high-fat diet (HFD) destroys the intestinal mucosal barrier by damaging intestinal stem cells (ISCs). A HFD can increase the concentration of intestinal deoxycholic acid (DCA) and decrease the secretion of interleukin-22 (IL-22), which plays an important role in the proliferation, repair and regeneration of ISCs. We hypothesized that increased level of intestinal DCA induced by a HFD leads to ISC dysfunction by reducing the IL-22 levels in intestinal tissues. In this study, 2 weeks of a DCA diet or a HFD damaged ileal ISC and its proliferation and differentiation, resulting in a decrease in Paneth cells and goblet cells. Importantly, 2 weeks of a DCA diet or a HFD also reduced ileal IL-22 concentration, accompanied by a decreased number of group 3 innate lymphoid cells in ileal mucosa, which produce IL-22 after intestinal injury. Concurrent feeding with bile acid binder cholestyramine prevented all these changes induced by a HFD. In addition, in vitro study further confirmed that exogenous IL-22 reversed the decline in the proliferation and differentiation of ileal ISCs induced by DCA stimulation. Collectively, these results revealed that the decrease in intestinal IL-22 induced by DCA may be a novel mechanism by which HFD damages ISCs. The administration of IL-22 or a bile acid binder may provide novel therapeutic targets for the metabolic syndrome caused by a HFD.
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http://dx.doi.org/10.1016/j.bbrc.2021.09.061DOI Listing
November 2021

Targeted elimination of myeloid-derived suppressor cells via regulation of the STAT pathway alleviates tumor immunosuppression in neuroblastoma.

Immunol Lett 2021 Sep 30;240:31-40. Epub 2021 Sep 30.

Departments of Pediatric Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China.

Neuroblastoma (NB) has high morality rates and is the most common malignant tumor found in children. High aggregation of myeloid-derived suppressor cells (MDSCs) in the tumor microenvironment results in immunosuppression and affects therapeutic effectiveness. At present, doxorubicin (DOX) and dopamine (DA) are the specific drugs used to selectively remove or mature MDSCs. The aim of the present study was to explore the feasibility and underlying mechanism of targeting elimination of MDSCs via DOX or DA administration to alleviate tumor immunosuppression in NB. In the present study, a BALB/c tumor-bearing mouse model was established, and mice were grouped into the control, DOX2.5, DOX5 and DA50 mg/kg groups. DOX or DA were injected intravenously on days 7 and 12 after inoculation, following which the parameters related to the signal transducer and activator of transcription (STAT) pathway in MDSCs, the proportion of MDSCs, T cell infiltration, programmed death-1 (PD-1) on the surface of T cells, the number of regulatory T cells (Tregs), polarization of tumor-related macrophages (TAMs) and tumor growth were compared between the groups on days 14, 17 and 23 after inoculation. The results demonstrated that following DOX or DA administration, STAT1/phosphorylated (p)-STAT1 decreased, whereas STAT3/p-STAT3, STAT5/p-STAT5 and STAT6/p-STAT6 increased, which was accompanied by a decrease in the MDSC proportion in each experimental group. Simultaneously, T cell infiltration in tumors was increased, whereas expression of PD-1, the number of Tregs, TAM polarization and tumor growth were inhibited. The most significant findings were observed in the DOX2.5 mg/kg group. To conclude, low dose DOX or DA administration could effectively regulate the STAT pathway to eliminate MDSCs, alleviate immunosuppression and improve the immune response against NB tumor cells.
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http://dx.doi.org/10.1016/j.imlet.2021.09.011DOI Listing
September 2021

Influence of mixture of spices on phospholipid molecules during water-boiled salted duck processing based on shotgun lipidomics.

Food Res Int 2021 11 21;149:110651. Epub 2021 Aug 21.

China Light Industry Key Laboratory of Meat Microbial Control and Utilization, Hefei University of Technology, Hefei 230009, China; School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China; Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230601, China. Electronic address:

This study aimed to evaluate the influence of spices on individual phospholipid molecules of water-boiled salted duck (WSD) processing. Shotgun lipidomics was used to determine the structure of individual phospholipid molecules in raw duck meat and changes of phospholipids in processed-WSD with or without spices. A total of 118 phospholipid molecules were determined during the whole processing. Spices had a significant effect on the changes of most individual phospholipid molecules during the processing, but the overall effect on the phospholipid profile was not obvious. Nine phospholipid molecule markers were screened by partial least squares discriminant analysis, which can be used to distinguish with or without spice treatment. The effect of spices on most phospholipid molecules began on the first day of dry-ripening, and gradually became more obvious in the subsequent processing. Spice's main function was to delay the degradation of individual phospholipid molecules.
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http://dx.doi.org/10.1016/j.foodres.2021.110651DOI Listing
November 2021

Diffuse painful erythema on right thigh.

JAAD Case Rep 2021 Oct 23;16:120-123. Epub 2021 Aug 23.

Department of Dermatology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

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http://dx.doi.org/10.1016/j.jdcr.2021.06.032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452784PMC
October 2021

[Effects of Combination of Organic and Inorganic Nitrogen on Nitrification and Denitrification in Two Salinized Soils].

Huan Jing Ke Xue 2021 Oct;42(10):5010-5020

College of Water Conservancy and Civil Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China.

Focusing on typical mildly saline soil, S (EC, 0.62 dS·m), and moderately saline soil, S (EC, 1.17 dS·m), in Hetao Irrigation District of Inner Mongolia, the response of nitrification and denitrification process to salinization degree and the proportion of organic and inorganic nitrogen application were studied. The experimental treatments consisted of(1) no nitrogen(CK), (2) only inorganic nitrogen(U), and(3) organic nitrogen(UO, UO, UO, and O) compared to 25%, 50%, 75%, and 100% inorganic nitrogen. The results showed that increasing salinity reduced the soil nitrification potential and increased the soil denitrification capacity. The soil nitrification potential of the S soil was 28.81%-69.67% higher than that of the S soil, while the denitrification capacity was reduced by 17.16%-88.91%. With an increase in salinity, the AOB abundance and nitrification contribution rate were reduced, but the AOA abundance and nitrification contribution rate were increased. Furthermore, an increase in salinity increased the abundance of and bacteria, and increased NO/(NO+N) production, but reduced the abundance of . In the S soil, the nitrification potential and denitrification capacity of UO were highest, increasing by as much as 18.59% and 15.87%, respectively, compared to the U treatment. In the S soil, the difference in the soil nitrification potential between the various fertilization treatments was not significant, and the denitrification capacity of the O treatment was highest. The S and S saline soils treated with UO and O, respectively, had higher AOB gene abundance and nitrification contribution rates, increased and gene abundances, and significantly reduced NO/(NO+N) product ratios. Our findings suggested that mildly saline soils(120 kg·hm urea+120 kg·hm organic fertilizer) and moderately saline soils(240 kg·hm organic fertilizer) are more conducive to soil nitrification and denitrification processes compared to soils to which inorganic nitrogen is applied alone.
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http://dx.doi.org/10.13227/j.hjkx.202103013DOI Listing
October 2021

Recent advances in therapeutic nucleic acids and their analytical methods.

J Pharm Biomed Anal 2021 Nov 16;206:114368. Epub 2021 Sep 16.

Institute of Drug Metabolism and Pharmaceutical Analysis, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China; Cancer Center of Zhejiang University, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, Hangzhou, China. Electronic address:

Therapeutic nucleic acids are various chemically modified RNA or DNA with different functions, which mainly play roles at the gene level. Owing to its accurately targeting at pathogenic genes, nucleic acid based therapeutics have a wide range of application prospects. Recently, the improvement on chemical synthesis and delivery materials accelerated the development of therapeutic nucleic acids rapidly. Up to now, 17 nucleic acid based therapeutics approved by Food and Drug Administration (FDA) or European Medicines Agency (EMA). The development of therapeutics raised higher requirements for analytical methods, both in quality control and in clinical research. The first part of this review introduces different classes of therapeutic nucleic acids, including antisense oligonucleotide (ASO), RNA interference (RNAi) therapy, mRNA, aptamer and other classes which are under research. The second part reviews the therapeutic nucleic acids commercialized from 2019 to now. The third part discusses the analytical methods for nucleic acid based therapeutics, including liquid chromatography-based methods, capillary gel electrophoresis (CGE), hybridization enzyme-linked immunosorbent assay (ELISA) and other infrequently used methods. Finally, the advantages and shortcomings of these methods are summarized, and the future development of analysis methods are prospected.
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http://dx.doi.org/10.1016/j.jpba.2021.114368DOI Listing
November 2021

OncoSplicing: an updated database for clinically relevant alternative splicing in 33 human cancers.

Nucleic Acids Res 2021 Sep 23. Epub 2021 Sep 23.

Institute of Urology of Hubei Province, Wuhan 430030, China.

Alternative splicing (AS) represents a crucial method in mRNA level to regulate gene expression and contributes to the protein complexity. Abnormal splicing has been reported to play roles in several diseases, including cancers. We developed the OncoSplicing database for visualization of survival-associated and differential alternative splicing in 2019. Here, we provide an updated version of OncoSplicing for an integrative view of clinically relevant alternative splicing based on 122 423 AS events across 33 cancers in the TCGA SpliceSeq project and 238 558 AS events across 32 cancers in the TCGA SplAdder project. The new version of the database contains several useful features, such as annotation of alternative splicing-associated transcripts, survival analysis based on median and optimal cut-offs, differential analysis between TCGA tumour samples and adjacent normal samples or GTEx normal samples, pan-cancer views of alternative splicing, splicing differences and results of Cox'PH regression, identification of clinical indicator-relevant and cancer-specific splicing events, and downloadable splicing data in the SplAdder project. Overall, the substantially updated version of OncoSplicing (www.oncosplicing.com) is a user-friendly and registration-free database for browsing and searching clinically relevant alternative splicing in human cancers.
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http://dx.doi.org/10.1093/nar/gkab851DOI Listing
September 2021

Label-free quantification of soft tissue alignment by polarized Raman spectroscopy.

Acta Biomater 2021 Sep 17. Epub 2021 Sep 17.

J. Crayton Pruitt Family Department of Biomedical Engineering, Herbert Wertheim College of Engineering, University of Florida, Gainesville, Florida, USA. Electronic address:

The organization of proteins is an important determinant of functionality in soft tissues. However, such organization is difficult to monitor over time in soft tissue with complex compositions. Here, we establish a method to determine the alignment of proteins in soft tissues of varying composition by polarized Raman spectroscopy (PRS). Unlike most conventional microscopy methods, PRS leverages non-destructive, label-free sample preparation. PRS data from highly aligned muscle layers were utilized to derive a weighting function for aligned proteins via principal component analysis (PCA). This trained weighting function was used as a master loading function to calculate a principal component score (PC1 Score) as a function of polarized angle for tendon, dermis, hypodermis, and fabricated collagen gels. Since the PC1 Score calculated at arbitrary angles was insufficient to determine level of alignment, we developed an Amplitude Alignment Metric by fitting a sine function to PC1 Score with respect to polarized angle. We found that our PRS-based Amplitude Alignment Metric can be used as an indicator of level of protein alignment in soft tissues in a non-destructive manner with label-free preparation and has similar discriminatory capacity among isotropic and anisotropic samples compared to microscopy-based image processing method. This PRS method does not require a priori knowledge of sample orientation nor composition and appears insensitive to changes in protein composition among different tissues. The Amplitude Alignment Metric introduced here could enable convenient and adaptable evaluation of protein alignment in soft tissues of varying protein and cell composition. STATEMENT OF SIGNIFICANCE: Polarized Raman spectroscopy (PRS) has been used to characterize the of organization of soft tissues. However, most of the reported applications of PRS have been on collagen-rich tissues and reliant on intensities of collagen-related vibrations. This work describes a PRS method via a multivariate analysis to characterize alignment in soft tissues composed of varying proteins. Of note, the highly aligned muscle layer of mouse skin was used to train a master function then applied to other soft tissue samples, and the degree of anisotropy in the PRS response was evaluated to obtain the level of alignment in tissues. We have demonstrated that this method supports convenient and adaptable evaluation of protein alignment in soft tissues of varying protein and cell composition.
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http://dx.doi.org/10.1016/j.actbio.2021.09.015DOI Listing
September 2021

Two-dimensional molybdenum carbide 2D-MoC as a superior catalyst for CO hydrogenation.

Nat Commun 2021 Sep 17;12(1):5510. Epub 2021 Sep 17.

Department of Mechanical and Process Engineering, ETH Zürich, CH 8092, Zürich, Switzerland.

Early transitional metal carbides are promising catalysts for hydrogenation of CO. Here, a two-dimensional (2D) multilayered 2D-MoC material is prepared from MoCT of the MXene family. Surface termination groups T (O, OH, and F) are reductively de-functionalized in MoCT (500 °C, pure H) avoiding the formation of a 3D carbide structure. CO hydrogenation studies show that the activity and product selectivity (CO, CH, C-C alkanes, methanol, and dimethyl ether) of MoCT and 2D-MoC are controlled by the surface coverage of T groups that are tunable by the H pretreatment conditions. 2D-MoC contains no T groups and outperforms MoCT, β-MoC, or the industrial Cu-ZnO-AlO catalyst in CO hydrogenation (evaluated by CO weight time yield at 430 °C and 1 bar). We show that the lack of surface termination groups drives the selectivity and activity of Mo-terminated carbidic surfaces in CO hydrogenation.
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http://dx.doi.org/10.1038/s41467-021-25784-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8448824PMC
September 2021

Water quality and health risk assessment of the water bodies in the Yamdrok-tso basin, southern Tibetan Plateau.

J Environ Manage 2021 Dec 13;300:113740. Epub 2021 Sep 13.

School of Environmental Science and Engineering, Southern University of Science and Technology, 518055, Shenzhen, China; Shenzhen Municipal Engineering Lab of Environmental IoT Technologies, Southern University of Science and Technology, 518055, Shenzhen, China.

Water resources in good quality guarantee the primary condition for the maintenance and development of the natural ecosystem and human society. Water quality status and health risk of the lake water bodies in the national nature reserve, the Yamdrok-tso basin, in the southern Tibetan Plateau are assessed by 25 water parameters including 12 heavy metal(loid)s. Results reveal that the lake water bodies possess relatively high pH (9.68), high concentrations of F (1.66 mg/L), Cu (13.92 μg/L), As (41.60 μg/L), Pb (26.69 μg/L), and U (19.53 μg/L), and a low value of dissolved oxygen (19.30%). The pollution indices (heavy metal pollution index of 0.88-22.88, heavy metal evaluation index of 0.18-3.75, and the degree of contamination of -8.82 to -5.25) demonstrate that the lake water bodies are in a low pollution level with respect to heavy metal(loid)s. The evaluation of water quality based on the fuzzy comprehensive assessment method suggests that 75.56% of the water samples meet the regulation of the China National Standard for water resources in national nature reserves. Health risk assessment shows that potential hazards exist on this region when the residents under long-term exposure to the lake water through oral and dermal pathways, of which children and adults are mostly exposed to As and F for non-carcinogenic and As for carcinogenic risks, especially for children. Results of this study contribute to targeted water resources management in the national nature reserves.
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http://dx.doi.org/10.1016/j.jenvman.2021.113740DOI Listing
December 2021

Oligopeptides from Jinhua ham prevent alcohol-induced liver damage by regulating intestinal homeostasis and oxidative stress in mice.

Food Funct 2021 Oct 19;12(20):10053-10070. Epub 2021 Oct 19.

School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China.

The current study aimed to evaluate the protective activity of peptides isolated from Jinhua ham (JHP) against alcoholic liver disease (ALD) and the mechanisms by which JHP prevents against ALD. The tangential flow filtration (TFF) combined with size exclusion chromatography (SEC) and reversed-phase high performance liquid chromatography (RP-HPLC) were used to isolate the JHP. Then the hepatoprotective activity of peptides was evaluated through experiments in mice. The primary structure of the peptide with the strongest liver protective activity was Lys-Arg-Gln-Lys-Tyr-Asp (KRQKYD) and the peptide was derived from the myosin of Jinhua ham, which were both identified by LC-MS/MS. Furthermore, the mechanism of KRQKYD prevention against ALD was attributed to the fact that KRQKYD increases the abundance of in the gut and decreases the abundance of Proteobacteria (especially ). The LPS-mediated liver inflammatory cascade was reduced by protecting the intestinal barrier, increasing the tight connection of intestinal epithelial cells and reducing the level of LPS in the portal venous circulation. KRQKYD could inhibit the production of ROS by upregulating the expression of the / antioxidant defense system and by reducing oxidative stress injury in liver cells. This study can provide a theoretical foundation for the application of JHP in the protection of liver from ALD.
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http://dx.doi.org/10.1039/d1fo01693hDOI Listing
October 2021

Role of Bclaf1 in Promoting Adrenocortical Carcinoma Proliferation: A Study Combining the Use of Bioinformatics and Molecular Events.

Cancer Manag Res 2021 31;13:6785-6795. Epub 2021 Aug 31.

Department of Occupational Health and Occupational Medicine, School of Public Health, Southern Medical University, Guangzhou, People's Republic of China.

Purpose: Adrenocortical carcinoma (ACC) is a rare malignancy with poor prognosis, and researchers are interested in further studying its diagnosis and treatment. Our study aims to identify new potential therapeutic targets in ACC.

Patients And Methods: The core genes CDK1 and CCNB1 were previously screened using ACC data from The Cancer Genome Atlas (TCGA) as the most relevant to Bclaf1 and tumour prognosis. We used siRNA- or shRNA-based models to explore the role of Bcl-2-associated transcription factor 1 (Bclaf1) in SW-13 cell lines. Western blotting and qPCR were used to determine the effects of Bclaf1 on CDK1 and Cyclin B1.

Results: Based on biological information analysis, we found that Bcl-2-associated transcription factor 1 (Bclaf1) affected the progression of ACC and was associated with the cell cycle. Downregulated Bclaf1 expression inhibited the proliferation of SW-13 cells and affected the cell cycle process of SW-13 cells. BCLAF1 was correlated with CDK1 and CCNB1 and can regulate their mRNA and protein levels.

Conclusion: Bclaf1 might promote the development of ACC by regulating CDK1 and Cyclin B1 to drive mitosis.
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http://dx.doi.org/10.2147/CMAR.S316599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418367PMC
August 2021

Molecular dynamics study of CDC25B mutant causing the activity decrease of CDC25B.

J Mol Graph Model 2021 Sep 6;109:108030. Epub 2021 Sep 6.

Tianjin Key Laboratory of Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin, 300070, People's Republic of China. Electronic address:

Cell division cycle 25B (CDC25B) was responsible for regulating the various stages of cell division in the cell cycle. R492L was one of the common types of CDC25B mutants. Researches showed that compared to CDC25B, CDC25B mutant had a ∼100-fold reduction in the rate constant for forming phosphatase intermediate (k). However, the molecular basis of how the CDC25B mutant influenced the process of binding between CDC25B and CDK2/CyclinA was not yet known. Therefore, the optimizations of three-dimensional structure of the CDC25B-CDK2/CyclinA system and the CDC25B-CDK2/CyclinA system were constructed by ZDOCK and RDOCK, and five methods were employed to verify the reasonability of the docking structure. Then the molecular dynamics simulations on the two systems were performed to explore the reason why CDC25B mutant caused the weak interactions between CDC25B and CDK2/CyclinA, respectively. The remote docking site (Arg488-Tyr497) and the second active site (Lys538-Arg544) of CDC25B were observed to have high fluctuations in the CDC25B-CDK2/CyclinA system with post-analysis, where the high fluctuation of these two regions resulted in weak interactions between CD25B and CDK2. In addition, Asp38-Glu42 and Asp206-Asp210 of CDK2 showed the slightly descending fluctuation, and CDK2 revealed an enhanced the self-interaction, which made CDK2 keep a relatively stable state in the CDC25B-CDK2/CyclinA system. Finally, Leu492 of CDC25B was speculated to be the key residue, which had great effects on the binding between CDC25B and CDK2 in the CDC25B-CDK2/CyclinA system. Consequently, overall analyses appeared in this study ultimately offered a helpful understanding of the weak interactions between CDC25B and CDK2.
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http://dx.doi.org/10.1016/j.jmgm.2021.108030DOI Listing
September 2021

Liubao brick tea activates the PI3K-Akt signaling pathway to lower blood glucose, metabolic disorders and insulin resistance via altering the intestinal flora.

Food Res Int 2021 10 10;148:110594. Epub 2021 Jul 10.

Institute of Food Engineering, College of Life Sciences, Shanghai Normal University, 100 Guilin Road, Xuhui District, Shanghai 200234, China. Electronic address:

Diabetes and its related metabolic disorders are worldwide public health issues. Many studies have shown that changes in the structure and composition of the intestinal flora are closely related to the host's physiological and pathological processes. In this study, we aim to explore the effect of Liubao tea (LBT) extract on hyperglycemic mice with metabolic disorders and intestinal flora dysbiosis and to further study its regulatory effect on insulin resistance and its potential regulatory mechanism. Our results show that LBT had a good hypoglycemic effect and could significantly alleviate the metabolic disorder evoked by hyperglycemia. The gut microbial sequencing showed that LBT treatment increased the diversity of intestinal flora, increased the abundance of beneficial bacteria, and reduced the abundance of harmful or conditional pathogenic bacteria, as well as significantly altered 39 of the top 50 OTUs with abundance. Besides, LBT could activate the PI3K-Akt-PPARs-GLUT2 cascade signaling pathway to improve metabolic disorders, thereby alleviating insulin resistance. These results suggest that LBT has excellent potential to become a natural functional food for the prevention of hyperglycemia and insulin resistance.
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http://dx.doi.org/10.1016/j.foodres.2021.110594DOI Listing
October 2021
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