Publications by authors named "Hui Wang"

8,096 Publications

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Localized management of non-indigenous animal domesticates in Northwestern China during the Bronze Age.

Sci Rep 2021 Aug 3;11(1):15764. Epub 2021 Aug 3.

Department of Anthropology, Washington University in St. Louis, 1 Brookings Dr., St. Louis, MO, 63130, USA.

The movements of ancient crop and animal domesticates across prehistoric Eurasia are well-documented in the archaeological record. What is less well understood are the precise mechanisms that farmers and herders employed to incorporate newly introduced domesticates into their long-standing husbandry and culinary traditions. This paper presents stable isotope values (δC, δN) of humans, animals, and a small number of plants from the Hexi Corridor, a key region that facilitated the movement of ancient crops between Central and East Asia. The data show that the role of animal products in human diets was more significant than previously thought. In addition, the diets of domestic herbivores (sheep/goat, and cattle) suggest that these two groups of domesticates were managed in distinct ways in the two main ecozones of the Hexi Corridor: the drier Northwestern region and the wetter Southeastern region. Whereas sheep and goat diets are consistent with consumption of naturally available vegetation, cattle exhibit a higher input of C plants in places where these plants contributed little to the natural vegetation. This suggests that cattle consumed diets that were more influenced by human provisioning, and may therefore have been reared closer to the human settlements, than sheep and goats.
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http://dx.doi.org/10.1038/s41598-021-95233-xDOI Listing
August 2021

Characteristics of Fertility Transition Response to the Cumulative Effective Low Temperature in a Two-Line Male Sterile Rice Cultivar.

Rice (N Y) 2021 Aug 3;14(1):71. Epub 2021 Aug 3.

National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou, 510642, People's Republic of China.

Background: Photo-thermo-sensitive genic male sterile (PTGMS) rice (Oryza sativa L.) is usually considered two-line male sterile rice because of its dual-purpose in two-line hybrid rice system: under short days and low temperatures, it is fertile and used for self-propagation, but under long days and high temperatures, it is sterile and used for hybrid seed production. Therefore, photoperiod and temperature conditions are extremely important for the fertility transition of two-line male sterile rice. In recent years, there have been frequent occurrences of abnormally low-temperature (ALT) resulting in failure of two-line hybrid rice seed production. The daily average temperature (DAT) during ALT events is sometimes higher than the critical sterility-inducing temperature (CSIT) of two-line male sterile rice, of which the night temperature is lower than the CSIT. DAT has been traditionally used as the single indicator of pollen fertility transition, but it is unknown why the fertility of two-line male sterile rice in seed production restored fertility under ALT conditions.

Results: For Hang93S (H93S), a newly released PTGMS line, we hypothesized fertility transition is determined mainly by the cumulative effective low temperature (ELT) and only a certain duration of low temperature is required every day during the fertility-sensitive period. This study simulated ALTs where the DAT was higher than the CSIT while some segments of night temperature were lower than the CSIT. The results showed H93S exhibited a fertility transition to varying degrees. Moreover, fertility was restored under simulated ALT conditions and pollen fertility increased with increasing cumulative ELT, indicating that the fertility transition was affected primarily by the cumulative ELT. Results also indicated that pollen fertility increased as the number of treatment days increased.

Conclusions: The fertility transition is caused mainly by the cumulative ELT. In two-line male sterile rice breeding, the effects of day length, ALT at night, and continuous response days should be considered together. The present study provides new insight into fertility transition so breeders can more effectively utilize the two-line male sterile rice, H93S, in breeding programs.
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http://dx.doi.org/10.1186/s12284-021-00514-8DOI Listing
August 2021

An analysis of curative effect of combined transvaginal and hysteroscopic electrocauterization of partial endometrium to treat previous cesarean scar diverticulum.

Minerva Surg 2021 Aug 3. Epub 2021 Aug 3.

Department of Gynecology and Obstetrics, The Third Affiliated Hospital of Soochow University, Changzhou, China.

Background: To explore the curative effect of the combined transvaginal and hysteroscopic electrocauterization of partial endometrium to treat previous cesarean scar diverticulum.

Methods: The retrospective study method is adopted. 28 cases of cesarean scar diverticulum patients were selected as research objects and divided into two groups: one is combined hysteroscopic and transvaginal surgery group (n=15) and the other is hysteroscopic electrocauterization group (n=13). The curative effects of the two groups are observed and compared, including clinical treatment indicators (operation duration, intraoperative blood loss, fever, length of hospital stay (LOS), etc.), and follow-up visits are carried out to check the differences of diverticulum repair and menostaxis improvement one to six months after surgery.

Results: The average operation duration, length of stay, and hospitalization expenses for the hysteroscopic electrocauterization group are respectively (28.84 ± 12.61) min, (2.93 ± 1.04) d, and (7916.57 ± 1948.45) yuan, all significantly lower than the combined hysteroscopic and transvaginal surgery group, those are respectively 73.93 ± 15.46 min, 5.93 ± 1.22 d, and 10,130.10 ± 1,037.75 yuan. The differences are statistically significant (P < 0.05). The follow-up visits in six months after operation find that the effective rate of menstrual period (menostaxis) improvement of the combined hysteroscopic and transvaginal surgery group is 93.3% (14 / 15), and that of scar diverticulum repair is 73.3% (11/15), which are notably higher than the hysteroscopic electrocauterization group, those are respectively 46.2% (6/13) and 23.1% (3/13). The differences are also statistically significant (P < 0.05).

Conclusions: The repairing effect of the combined transvaginal and hysteroscopic electrocauterization of partial endometrium to treat previous cesarean scar diverticulum [previous cesarean scar defect (PCSD)] is obvious. As it can fully remove previous cesarean scars and the surrounding ectopic intimae and reduce the recurrence rate, it is worthy of clinical promotion and application.
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http://dx.doi.org/10.23736/S2724-5691.21.08999-1DOI Listing
August 2021

Quantitative analysis of renal blood flow during thoracic endovascular aortic repair in type B aortic dissection using iFlow.

Quant Imaging Med Surg 2021 Aug;11(8):3726-3734

State Key Laboratory of Cardiovascular Disease, Center of Vascular Surgery, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Currently, the thoracic endovascular aortic repair is the recommended clinical treatment for type B aortic dissections. Unfortunately, malperfusion or ischemia of the kidneys is a major complication of type B aortic dissections. Despite this, few studies have focused on the effects of thoracic endovascular aortic repair on blood flow in renal arteries and parenchyma. This current investigation used novel real-time imaging software to quantitatively analyze the hemodynamic changes in renal artery blood flow and perfusion before and after stent graft placement.

Methods: A total of 51 patients with type B aortic dissection undergoing thoracic endovascular aortic repair between April 2017 and September 2019 were retrospectively recruited. The pre-and post-procedural digital subtraction angiography images were converted into color-coded maps using iFlow for quantitative comparison. Time-intensity curves and related parameters, including the average peak ratio (avg.Pr), average delayed time to peak (avg.dTTP), and average area under the curve ratio (avg.AUCr) of the renal arteries and renal cortex were obtained and analyzed. Wilcoxon signed-rank test was used to compare iFlow parameters before and after endovascular repair. Spearman correlation analyses were performed to study iFlow parameters and renal function parameters and the estimated glomerular filtration rate (eGFR) and blood urea nitrogen (BUN).

Results: A total of 102 images including 51 pre-operative and 51 post-operative image datasets were successfully post-processed. Following endovascular repair, iFlow showed a significant 33.0% increase in avg.Pr (P<0.001) and a significant 35.1% increase in avg.AUCr (P<0.001) in the renal artery. Additionally, there was a significant 12.2% decrease in the avg.dTTP (P=0.001), a significant 24.5% increase in avg.Pr (P=0.004), and a significant 38.3% increase in avg.AUCr (P=0.009) in the renal cortex. Spearman correlation analysis showed that after endovascular repair there was a significant correlation between the avg.Pr of the renal artery and eGFR (r=0.30; P=0.0349), the avg.Pr of the renal cortex and eGFR (r=0.30; P=0.0300), and the avg. AUCr of the renal cortex and BUN (r=0.31; P=0.0289).

Conclusions: iFlow provided a novel quantitative method for evaluating renal hemodynamic changes in patients with type B aortic dissection undergoing endovascular treatment. Time-intensity curve parameters may facilitate the intraprocedural evaluation of renal blood flow and perfusion to complement the color-coded map.
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http://dx.doi.org/10.21037/qims-20-992DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245938PMC
August 2021

Tumor elastography and its association with cell-free tumor DNA in the plasma of breast tumor patients: a pilot study.

Quant Imaging Med Surg 2021 Aug;11(8):3518-3534

Shenzhen Key Laboratory of Viral Oncology, Center for Clinical Research and Innovation (CCRI), Shenzhen Hospital, Southern Medical University, Shenzhen, China.

Background: Breast tumor stiffness, which can be objectively and noninvasively evaluated by ultrasound elastography (UE), has been useful for the differentiation of benign and malignant breast lesions and the prediction of clinical outcomes. Liquid biopsy analyses, including cell-free tumor DNA (ctDNA), exhibit great potential for personalized treatment. This study aimed to investigate the correlations between the UE and ctDNA for early breast cancer diagnosis.

Methods: Breast tumor stiffness in 10 patients were assessed by shear wave elastography (SWE), and the ctDNA of eight collected plasma specimens with different tumor stiffness were analyzed by whole-genome sequencing (WGS). Subsequently, the distribution of carcinoma-associated fibroblasts (CAFs) was investigated by detecting the expression levels of alpha-smooth muscle actin (α-SMA) in tissues of breast lesions. We validated the function of discoidin domain receptor 2 (DDR2) in breast tumor CAFs by knockout of fibroblast activation protein (FAP) with different tumor stiffness during cancer progression and .

Results: The UE estimates of tumor stiffness positively correlated with CAF-rich (α-SMA) tumors (P<0.05). Copy number profiles and percent genome alterations were remarkably different between benign and malignant breast lesions. Somatic genomic alterations or structural variants of , , , and genes were identified in ctDNA of plasma from breast lesions with high SWE values and an increase in the CAF content obtained from clinical samples. Deletion of FAP in breast tumor CAFs by CRISPR/Cas9-mediated gene knockout and decreased tumor stiffness resulted in downregulated expression of (P<0.05), which in turn led to decreasing the tumor stiffness and carcinogenesis process and .

Conclusions: These results have established proof of principle that WGS analysis of ctDNA could complement current UE approaches to assess tumor stiffness changes for the early diagnosis and prognostic assessment of breast cancer.
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http://dx.doi.org/10.21037/qims-20-443DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245960PMC
August 2021

Exploring the Clinical Characteristics of COVID-19 Clusters Identified Using Factor Analysis of Mixed Data-Based Cluster Analysis.

Front Med (Lausanne) 2021 16;8:644724. Epub 2021 Jul 16.

Department of Integrated Chinese Traditional and Western Medicine, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, China.

The COVID-19 outbreak has brought great challenges to healthcare resources around the world. Patients with COVID-19 exhibit a broad spectrum of clinical characteristics. In this study, the Factor Analysis of Mixed Data (FAMD)-based cluster analysis was applied to demographic information, laboratory indicators at the time of admission, and symptoms presented before admission. Three COVID-19 clusters with distinct clinical features were identified by FAMD-based cluster analysis. The FAMD-based cluster analysis results indicated that the symptoms of COVID-19 were roughly consistent with the laboratory findings of COVID-19 patients. Furthermore, symptoms for mild patients were atypical. Different hospital stay durations and survival differences among the three clusters were also found, and the more severe the clinical characteristics were, the worse the prognosis. Our aims were to describe COVID-19 clusters with different clinical characteristics, and a classifier model according to the results of FAMD-based cluster analysis was constructed to help provide better individualized treatments for numerous COVID-19 patients in the future.
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http://dx.doi.org/10.3389/fmed.2021.644724DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8323882PMC
July 2021

Sinensetin Reduces Osteoarthritis Pathology in the Tert-Butyl Hydroperoxide-Treated Chondrocytes and the Destabilization of the Medial Meniscus Model Mice the AMPK/mTOR Signaling Pathway.

Front Pharmacol 2021 16;12:713491. Epub 2021 Jul 16.

Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.

As a common degenerative disease, osteoarthritis (OA) usually causes disability in the elderly and socioeconomic burden. Previous studies have shown that proper autophagy has a protective effect on OA. Sinensetin (Sin) is a methylated flavonoid derived from citrus fruits. Studies have shown that Sin is a good autophagy inducer and has shown excellent therapeutic effects in a variety of diseases; however, its role in the treatment of OA is not fully understood. This study proved the protective effect of Sin on OA through a series of and experiments. experiments have shown that Sin may inhibit chondrocyte apoptosis induced by tert-butyl hydroperoxide (TBHP); at the same time, it might also inhibit the production of MMP13 and promote the production of aggrecan and collagen II. Mechanism studies have shown that Sin promotes chondrocyte autophagy by activating AMPK/mTOR signaling pathway. On the contrary, inhibition of autophagy can partially abolish the protective effect of Sin on TBHP-treated chondrocytes. experiments show that Sin may protect against DMM-induced OA pathogenesis. These results provide evidence that Sin serves as a potential candidate for the treatment of OA.
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http://dx.doi.org/10.3389/fphar.2021.713491DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322586PMC
July 2021

Neuroprotective effect of combined use of nicotine and celecoxib by inhibiting neuroinflammation in ischemic rats.

Brain Res Bull 2021 Jul 29. Epub 2021 Jul 29.

Department of Nuclear Medicine, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address:

Introduction: The contribution of neuroinflammation in cognitive impairment is increasingly recognized. Non-steroidal anti-inflammatory drugs had been proven that it could improve cognitive impairment in large dose but with more side effect, which limited the application. The main objective of this study was to investigate whether the combined use of nicotine and celecoxib could obtain synergistic neuroprotective effect in ischemic rats.

Methods: Twenty adult Sprague-Dawley (SD) rats underwent ischemic model surgery by injecting endothelin-1 into the left thalamus, which were classified into four groups with different interventions: nicotine(1.5 mg/kg/d), celecoxib(15 mg/kg/d), nicotine(1.5 mg/kg/d) +celecoxib(15 mg/kg/d), or saline after surgery. The other five SD rats also underwent same surgery by injecting saline instead of endothelin-1, as the control group. Morris water maze (MWM) test was adopted to assess the cognition. Micro PET/CT with 2-[F]-A-85380 were performed for αβ-nAChRs detection in vivo. Western blot, real-time PCR and immunohistochemical staining were adopted to detect the expression of αβ-nAChRs and inflammatory factors which included TNF-α, IL-1β, IL-6 in brain tissue. Microglial activation in the brain was monitored by immunofluorescence with IBA1 staining.

Results: The MWM test showed rats given with nicotine or celecoxib alone showed much better memory than rats with saline, no difference was observed between nicotine and celecoxib. The rat memory was recovered most significant when the nicotine and celecoxib were combined (p < 0.05). Micro-PET/CT showed much more tracer uptake in the left thalamus and whole brain in rats given with nicotine, or nicotine + celecoxib (nico + cele group) than saline treated rats, whereas the rats given celecoxib did not. Compared with saline treated rats, we found the proteins of α nAChR and βnAChR in rats given nicotine or nico + cele increased significantly, and mRNA/proteins of TNF-α, IL-1β and IL-6 decreased at the same time. The α nAChR and β nAChR proteins in rats given celecoxib is the same as saline treated rats, whereas the inflammatory factors decreased obviously compared with saline treated rats. Microglial activation was confirmed in saline treated rats, which was inhibited in rats give nicotine, celecoxib or both.

Conclusions: The study revealed the combined use of nicotine and celecoxib may improve the cognitive function in ischemic rats, with a better effect than either alone. Both nicotine and celecoxib can inhibit inflammation, but through different mechanisms: nicotine can activate αβ-nAChRs while celecoxib is cyclooxygenase-2 inhibitor. Our findings suggest the combined application of two drugs with different anti-inflammation mechanism could attenuate cognitive impairment more effectively in ischemic rats, which may hold therapeutic potential in the clinical practice.
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http://dx.doi.org/10.1016/j.brainresbull.2021.07.022DOI Listing
July 2021

The influence of visual supports and motivation on motor performance of the MABC-2 for Chinese school-aged children with autism spectrum disorder.

Sci Rep 2021 Jul 30;11(1):15557. Epub 2021 Jul 30.

Collaborative Innovation Center of Assessment for Basic Education Quality, Beijing Normal University, Beijing, China.

The influences of including visual supports and strategies to increase motivation for children with autism spectrum disorder (ASD) in motor assessments were examined. 97 children with ASD and 117 age-matched typically developing (TD) children performed the Movement Assessment Battery for Children, Second Edition (MABC-2) under traditional, visual support, motivation, and visual support plus motivation protocols. Results showed that children with ASD elicited lower MABC-2 scores than TD children. Moreover, in children with ASD, the visual support protocol, but not the motivation protocol, produced higher scores on ball and balance skills than the traditional protocol. These findings indicated that children with ASD were developmentally delayed in motor skills; however, their performance may be improved by including visual supports in motor assessments.
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http://dx.doi.org/10.1038/s41598-021-95155-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8324853PMC
July 2021

Dynamics of heat transfer and moisture in beef jerky during hot air drying.

Meat Sci 2021 Jul 24;182:108638. Epub 2021 Jul 24.

College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China. Electronic address:

The aim of this study was to investigate the surface temperature, moisture migration, muscle shrinkage and microstructure of beef jerky during hot air multi-stage drying. Temperatures were sequentially increased from 40 to 50 to 60 °C, and corresponding times were 0.5 h - 1 h - 2.5 h, 0.5 h - 2 h - 1.5 h, 1 h - 1 h - 2 h and 1 h - 2 h - 1 h in 4 groups. With increasing temperature, moisture content and diffusivity of the sample decreased, the surface temperature, moisture migration, muscle shrinkage and the gaps (spacing) between muscle fibres increased. The jerky in groups 3 and 4 attained high overall acceptability. The jerky dried at 40 °C for 1 h, 50 °C for 1 h and 60 °C for 2 h had highest quality. The results cover the range of temperature and time used in beef drying and can be used to optimize the drying process of beef.
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http://dx.doi.org/10.1016/j.meatsci.2021.108638DOI Listing
July 2021

Secondary aerosol formation from a Chinese gasoline vehicle: Impacts of fuel (E10, gasoline) and driving conditions (idling, cruising).

Sci Total Environ 2021 Jul 6;795:148809. Epub 2021 Jul 6.

State Key Joint Laboratory of Environmental Simulation and Pollution Control, International Joint Laboratory for Regional Pollution Control, Ministry of Education (IJRC), College of Environmental Sciences and Engineering, Peking University, Beijing 100871, PR China; Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, Nanjing University of Information Science & Technology, Nanjing 210044, PR China.

Chassis dynamometer experiments were conducted to investigate the effect of vehicle speed and usage of ethanol-blended gasoline (E10) on formation and evolution of gasoline vehicular secondary organic aerosol (SOA) using a Gothenburg Potential Aerosol Mass (Go: PAM) reactor. The SOA forms rapidly, and its concentration exceeds that of primary organic aerosol (POA) at an equivalent photochemical age (EPA) of ~1 day. The particle effective densities grow from 0.62 ± 0.02 g cm to 1.43 ± 0.07 g cm with increased hydroxyl radical (OH) exposure. The maximum SOA production under idling conditions (4259-7394 mg kg-fuel) is ~20 times greater than under cruising conditions. There was no statistical difference between SOA formation from pure gasoline and its formation from E10. The slopes in Van Krevelen diagram indicate that the formation pathways of bulk SOA includes the addition of both alcohol/peroxide functional groups and carboxylic acid formation from fragmentation. A closure estimation of SOA based on bottom-up and top-down methods shows that only 16%-38% of the measured SOA can be explained by the oxidation of measured volatile organic compounds (VOCs), suggesting the existence of missing precursors, e.g. unmeasured VOCs and probably semivolatile or intermediate volatile organic compounds (S/IVOCs). Our results suggest that applying parameters obtained from unified driving cycles to model SOA concentrations may lead to large discrepancies between modeled and ambient vehicular SOA. No reduction in vehicular `SOA production is realized by replacing normal gasoline with E10.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148809DOI Listing
July 2021

Health inequities in COVID-19 vaccination among the elderly: Case of Connecticut.

J Infect Public Health 2021 Jul 22. Epub 2021 Jul 22.

Institute for Collaboration on Health, Intervention, and Policy (InCHIP), University of Connecticut, Storrs, CT 06269, USA; Department of Geography, University of Connecticut, Storrs, CT 06269, USA. Electronic address:

Background: In the United States, distribution plans for the COVID-19 vaccination were established at the state level. However, some states, such as Connecticut, followed an age-based strategy without considering occupations or co-morbid conditions due to its simplicity in implementation. This strategy raised concerns about exacerbating health inequities because it did not prioritize vulnerable communities, specifically, minorities and low-income groups. The study aims to examine the vaccination inequities among different population groups for people aged 65+.

Methods: A cross-sectional analysis of quantile-based independent sample t-test was employed to examine the relationship between eight social vulnerability indices (SVIs, i.e., below poverty, unemployed, without high school diploma, disability, minority, speaks English less than well, no vehicle, and mobile homes) and vaccination rates at the town level in Connecticut during the second phase of the vaccine distribution plan when individuals aged 65 and over were eligible. Negative binomial regressions were employed to further justify the relationships between SVIs and vaccination rates.

Results: The report shows that the differences in vaccination rates were statistically significant between the most vulnerable and the least vulnerable towns with respect to six SVIs (i.e., below poverty, without high school diploma, disability, minority, speaks English less than well, and no vehicle). The vaccination gap was greater for people aged 75+ than people aged 65-74. Among the selected SVIs, below poverty was negatively correlated with the vaccination rate for 75+, and without high school diploma was negatively correlated with both rates.

Conclusions: This report reveals the significant health inequities in COVID-19 vaccination among the elderly population at the early vaccination phase. It can shed insights into health policy initiatives to improve vaccination coverage in the elderly communities, such as promoting onsite scheduling and increasing at-home vaccination services.
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http://dx.doi.org/10.1016/j.jiph.2021.07.013DOI Listing
July 2021

Cadmium accelerates bacterial oleic acid production to promote fat accumulation in Caenorhabditis elegans.

J Hazard Mater 2021 Jul 22;421:126723. Epub 2021 Jul 22.

CAS Key Laboratory of Nutrition, Metabolism and Food safety, Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences, Shanghai 200025, China; State Key Laboratory of Oncogenes and Related Genes, Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Electronic address:

Environmental cadmium, with a high dietary intake and long biological half-life, is a severe health risk by harming physiological function directly or through gut microbiota. However, the toxicity mechanisms of environmental cadmium on microbes and host systems remain unclear. Herein, we established three C. elegans and E. coli cultivated systems to investigate the vital role of microorganisms in cadmium-induced lipid toxicity and depict the interaction between environmental cadmium, bacteria, and the host. We found that only nematodes in the system with live bacteria, rather than UV-killed bacteria or no bacteria, could be induced to fat accumulation by cadmium exposure, suggesting that bacteria mediated the effect of environmental cadmium on body fat. Cadmium caused perturbation of metabolite in bacteria, most notably oleic acid, elevated the synthesis genes expression, and enhanced the bacterial oleic acid production, which further promoted the expression of lipid metabolism-related genes and fat deposition in C. elegans regardless of the cultivated system. Finally, we showed the potential protective effect of Vitamin D3 which prevented cadmium- or oleic acid-induced fat storage significantly. In conclusion, this study illustrates the mechanism underlying cadmium-induced lipid accumulation in body through bacterial metabolites and reveals the interplay between environmental cadmium, microorganisms, and the host.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126723DOI Listing
July 2021

Elucidating the Relationship between ROS and Protein Phosphorylation through Fluorescence Imaging in the Pneumonia Mice.

Anal Chem 2021 Jul 29. Epub 2021 Jul 29.

College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Institute of Biomedical Sciences, Shandong Normal University, Jinan 250014, P. R. China.

Revealing the relationship between reactive oxygen species (ROS) and levels of protein phosphorylation is of great significance for understanding the pathogenesis of diseases. Although mass spectrometry is used as a classical method for protein phosphorylation analysis, there are still some challenges to realize protein phosphorylation recognition. Herein, we designed and prepared an metal-organic framework (MOF)-based fluorescent nanoprobe with Zr(IV) and boronate ester as an active center, which achieved simultaneous recognition of ROS and phosphorylation sites. The ROS unit was constructed by 1,8-naphthalimide and boronate ester as a fluorophore and a recognition group, respectively. The specific interaction between Zr(IV) and a phosphate group was used to realize fluorescence imaging of phosphorylation sites. Using the advantages of two-photon property of the ROS recognition unit, the nanoprobe can effectively reduce the background fluorescence and thus improve the imaging sensitivity. Finally, the MOF-based nanoprobe was successfully applied to reveal the relationship between ROS and levels of phosphorylation in pneumonia mice, which illustrated that the ROS and phosphorylation levels in the process of pulmonary inflammation were obviously higher than those of the normal mice. This work provides feasible fluorescence tools that have important significance for revealing pathogenesis of diseases.
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http://dx.doi.org/10.1021/acs.analchem.1c01690DOI Listing
July 2021

Rebalancing TGF-β/Smad7 signaling via Compound kushen injection in hepatic stellate cells protects against liver fibrosis and hepatocarcinogenesis.

Clin Transl Med 2021 Jul;11(7):e410

State Key Laboratory of Oncogenes and Related Genes, Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Liver fibrosis and fibrosis-related hepatocarcinogenesis are a rising cause for morbidity and death worldwide. Although transforming growth factor-β (TGF-β) is a critical mediator of chronic liver fibrosis, targeting TGF-β isoforms and receptors lead to unacceptable side effect. This study was designed to explore the antifibrotic effect of Compound kushen injection (CKI), an approved traditional Chinese medicine formula, via a therapeutic strategy of rebalancing TGF-β/Smad7 signaling.

Methods: A meta-analysis was performed to evaluate CKI intervention on viral hepatitis-induced fibrosis or cirrhosis in clinical randomized controlled trials (RCTs). Mice were given carbon tetrachloride (CCl ) injection or methionine-choline deficient (MCD) diet to induce liver fibrosis, followed by CKI treatment. We examined the expression of TGF-β/Smad signaling and typical fibrosis-related genes in hepatic stellate cells (HSCs) and fibrotic liver tissues by qRT-PCR, Western blotting, RNA-seq, immunofluorescence, and immunohistochemistry.

Results: Based on meta-analysis results, CKI improved the liver function and relieved liver fibrosis among patients. In our preclinical studies by using two mouse models, CKI treatment demonstrated promising antifibrotic effects and postponed hepatocarcinogenesis with improved liver function and histopathologic features. Mechanistically, we found that CKI inhibited HSCs activation by stabilizing the interaction of Smad7/TGF-βR1 to rebalance Smad2/Smad3 signaling, and subsequently decreased the extracellular matrix formation. Importantly, Smad7 depletion abolished the antifibrotic effect of CKI in vivo and in vitro. Moreover, matrine, oxymatrine, sophocarpine, and oxysophocarpine were identified as material basis responsible for the antifibrosis effect of CKI.

Conclusions: Our results unveil the approach of CKI in rebalancing TGF-β/Smad7 signaling in HSCs to protect against hepatic fibrosis and hepatocarcinogenesis in both preclinical and clinical studies. Our study suggests that CKI can be a candidate for treatment of hepatic fibrosis and related oncogenesis.
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http://dx.doi.org/10.1002/ctm2.410DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255064PMC
July 2021

Probable Causes and Risk Factors for Positive SARS-CoV-2 Testing in Recovered Patients: Evidence From Guangzhou, China.

Front Med (Lausanne) 2021 12;8:684101. Epub 2021 Jul 12.

Department of Epidemiology, School of Public Health, Southern Medical University, Guangzhou, China.

Some patients retested positive for SARS-CoV-2 following negative testing results and discharge. However, the potential risk factors associated with redetectable positive testing results in a large sample of patients who recovered from COVID-19 have not been well-estimated. A total of 745 discharged patients were enrolled between January 30, 2020, and September 9, 2020, in Guangzhou, China. Data on the clinical characteristics, comorbidities, drug therapy, RT-PCR testing, and contact modes to close contacts were collected. Patients who tested positive for SARS-CoV-2 after discharge were confirmed by guidelines issued by China. The repositive rate in different settings was calculated. Among 745 discharged patients, 157 (21.1%; 95% CI, 18.2-24.0%) tested repositive and the repositive rate was 16.8% (95% CI, 14.1-24.0%) for nasopharyngeal swabs and 9.7% (95% CI, 7.0-12.5%) for anal swabs. Among them, 55 (35.0%) were asymptomatic, 15 (9.6%) had mild symptoms, 83 (52.9%) had moderate symptoms, and 4 (2.6%) had severe symptoms at the first admission. The days from discharge to repositivity was 8.0 (IQR, 8.0-14.0). Most repositive patients were without clinical symptoms, and lymphocyte cell counts were higher than before being discharged. The likelihood of repositive testing for SARS-CoV-2 RNA was significantly higher among patients who were of younger age (OR, 3.88; 95% CI, 1.74-8.66, 0-17 years old), had asymptomatic severity (OR, 4.36; 95% CI, 1.47-12.95), and did not have clinical symptoms (OR, 1.89; 95% CI, 1.32-2.70, without fever). No other positive patients emerged within the families or close contacts of repositive patients. Our findings support prolonged but intermittent viral shedding as the probable cause for this phenomenon; we need to familiarize with the possibility that the virus will remain endemic.
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http://dx.doi.org/10.3389/fmed.2021.684101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311025PMC
July 2021

A dominant spotted leaf gene TaSpl1 activates endocytosis and defense-related genes causing cell death in the absence of dominant inhibitors.

Plant Sci 2021 Sep 21;310:110982. Epub 2021 Jun 21.

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Shaanxi, 712100, PR China; Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Shaanxi, 712100, PR China. Electronic address:

The spotted leaf lesion mimic trait simulates cell death in a plant responding to pathogen infection. Some spotted leaf genes are recessive, while others are dominant. A small number of plants with a lesion mimic phenotype appeared in a segregating population obtained by crossing two normal green wheat strains, XN509 and N07216. Here, we clarified the genetic model and its breeding value. Phenotyping of the consecutive progeny populations over six cropping seasons showed that the spotted leaf lesion mimic phenotype was controlled by a dominant gene designated TaSpl1, which was inhibited by two other dominant genes, designated TaSpl1-I and TaSpl1-I. Using bulked segregant analysis RNA-seq (BSR-Seq) and newly developed KASP-PCR markers, the TaSpl1 and TaSpl1-I loci in N07216 were mapped to the end of chromosomes 3DS and 3BS, respectively. Plants with the spotted phenotype showed lower levels of stripe rust and powdery mildew than those with the normal green phenotype. Compared with normal leaves, the differentially expressed genes in spotted leaves were significantly enriched in plant-pathogen interaction and endocytosis pathways. There were no differences in the yield parameters of the F and F sister lines, N13039S with TaSpl1 and N13039 N without TaSpl1. These results provide a greater understanding of spotted leaf phenotyping and the breeding value of the lesion mimic allele in developing disease-resistance varieties.
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http://dx.doi.org/10.1016/j.plantsci.2021.110982DOI Listing
September 2021

Critical role of VGLL4 in the regulation of chronic normobaric hypoxia-induced pulmonary hypertension in mice.

FASEB J 2021 08;35(8):e21822

Institute of Hypoxia Medicine, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, China.

Pulmonary hypertension (PH), a rare but deadly cardiopulmonary disorder, is characterized by extensive remodeling of pulmonary arteries resulting from enhancement of pulmonary artery smooth muscle cell proliferation and suppressed apoptosis; however, the underlying pathophysiological mechanisms remain largely unknown. Recently, epigenetics has gained increasing prominence in the development of PH. We aimed to investigate the role of vestigial-like family member 4 (VGLL4) in chronic normobaric hypoxia (CNH)-induced PH and to address whether it is associated with epigenetic regulation. The rodent model of PH was established by CNH treatment (10% O , 23 hours/day). Western blot, quantitative reverse transcription polymerase chain reaction, immunofluorescence, immunoprecipitation, and adeno-associated virus tests were performed to explore the potential mechanisms involved in CNH-induced PH in mice. VGLL4 expression was upregulated and correlated with CNH in PH mouse lung tissues in a time-dependent manner. VGLL4 colocalized with α-smooth muscle actin in cultured pulmonary arterial smooth muscle cells (PASMCs), and VGLL4 immunoactivity was increased in PASMCs following hypoxia exposure in vitro. VGLL4 knockdown attenuated CNH-induced PH and pulmonary artery remodeling by blunting signal transducer and activator of transcription 3 (STAT3) signaling; conversely, VGLL4 overexpression exacerbated the development of PH. CNH enhanced the acetylation of VGLL4 and increased the interaction of ac-H3K9/VGLL4 and ac-H3K9/STAT3 in the lung tissues, and levels of ac-H3K9, p-STAT3/STAT3, and proliferation-associated protein levels were markedly up-regulated, whereas apoptosis-related protein levels were significantly downregulated, in the lung tissues of mice with CNH-induced PH. Notably, abrogation of VGLL4 acetylation reversed CNH-induced PH and pulmonary artery remodeling and suppressed STAT3 signaling. Finally, STAT3 knockdown alleviated CNH-induced PH. In conclusion, VGLL4 acetylation upregulation could contribute to CNH-induced PH and pulmonary artery remodeling via STAT3 signaling, and abrogation of VGLL4 acetylation reversed CNH-induced PH. Pharmacological or genetic deletion of VGLL4 might be a potential target for therapeutic interventions in CNH-induced PH.
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http://dx.doi.org/10.1096/fj.202002650RRDOI Listing
August 2021

MicroRNA-93-5p participates in type 2 diabetic retinopathy through targeting Sirt1.

Int Ophthalmol 2021 Jul 27. Epub 2021 Jul 27.

Department of Ophthalmology, Shijiazhuang People's Hospital, No. 365, Jianhua South Street, Yuhua District, Shijiazhuang, 050030, Hebei Province, China.

Objective: To investigate the role of miR-93-5p in rats with type 2 diabetic retinopathy (DR) through targeting Sirt1.

Methods: The targeting correlation between miR-93-5p and Sirt1 was validated by dual-luciferase reporter gene assay. Type 2 diabetes mellitus (T2DM) rat models were received intravitreal injection of antagomir NC (negative control), miR-93-5p antagomir, miR-93-5p agomir and/or recombinant Sirt1, followed by observation of pathological changes in retina via HE staining. Besides, retinal vascular permeability was determined by fluorescein isothiocyanate-bovine serum albumin (FITC-BSA), while the retinal vasculature was observed through retinal trypsin digestion. Expression of miR-93-5p and Sirt1 was measured by qRT-PCR and Western blotting, while the levels of VEGF, proinflammatory cytokines and anti-oxidative indicators were determined using corresponding kits.

Results: MiR-93-5p could target Sirt1 as analyzed by the luciferase reporter gene assay. Rats in the T2DM group presented the up-regulation of miR-93-5p and down-regulation of Sirt1 in the retina, and miR-93-5p inhibition could up-regulate Sirt1 expression in the T2DM rats. Recombinant Sirt1 decreased retinal vascular permeability and acellular capillaries with improved pathological changes in retina from T2DM rats, which was abolished by miR-93-5p agomir. Moreover, miR-93-5p inhibition or Sirt1 overexpression decreased the levels of VEGF and proinflammatory cytokines while enhancing the activity of anti-oxidative indicators. However, indicators above had no significant differences between T2DM group and T2DM + agomir + Sirt1 group.

Conclusion: MiR-93-5p, via targeting Sirt1, could affect the vascular permeability and acellular capillaries and mitigate the inflammation and oxidative stress in the retinas, which may play a critical role in DR.
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http://dx.doi.org/10.1007/s10792-021-01953-4DOI Listing
July 2021

Identification of a Metacaspase Gene in the Bloom-Forming Dinoflagellate Prorocentrum minimum and its Putative Function Involved in Programmed Cell Death.

Curr Microbiol 2021 Jul 27. Epub 2021 Jul 27.

Department of Biotechnology, Sangmyung University, Seoul, 03016, South Korea.

Programmed cell death (PCD) in dinoflagellates has been introduced as a new concept that facilitates the demise of harmful algal blooms. Metacaspases (MCAs) play a role in PCD, but their function in dinoflagellates is unclear. Here, we cloned a novel MCA gene (PmMCA) from the harmful dinoflagellate Prorocentrum minimum and examined its molecular characteristics and gene expression during cell death. The gene was encoded in the nuclear genome with two introns. The putative protein contained 288 amino acids and three conserved MCA signature motifs. Phylogenetic analysis showed that PmMCA may have the same ancestor as other dinoflagellates. PmMCA expression and cell apoptosis were significantly induced under copper exposure, considerably affecting cell growth. These results suggest that PmMCA could be involved in PCD triggered by copper stress.
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http://dx.doi.org/10.1007/s00284-021-02617-3DOI Listing
July 2021

Copper-Catalyzed Synthesis of 5-Aryl-6-(Trifluoromethyl)-2,3-Dihydropyrazolo[1,2-a]Pyrazol-1(5H)-One.

Chem Asian J 2021 Jul 26. Epub 2021 Jul 26.

Soochow University, Testing and Analysis Center, CHINA.

An efficient protocol for the synthesis of 5-aryl-6-(trifluoromethyl)-2,3-dihydropyrazolo[1,2- a ]pyrazol-1(5 H )-one derivatives through a copper-catalyzed [3 + 2]-cycloaddition of azomethine imines with 3,3,3-trifluoropropyne (generated in situ from dehydrobromination of 2-bromo-3,3,3-trifluoropropene under base conditions) is developed. The advantages of this transformation are the broad substrate scope and the good functional group compatibility. The subsequent oxidation and nucleophilic substitution/aromatization provide a new approach to 4-trifluoromethylated pyrazol-1-yl propanoic acids.
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http://dx.doi.org/10.1002/asia.202100693DOI Listing
July 2021

Characteristics of patients with ST-segment elevated myocardial infarction (STEMI) at the initial stage of the COVID-19 pandemic: a systematic review and meta-analysis.

Infect Dis (Lond) 2021 Jul 27:1-11. Epub 2021 Jul 27.

Department of Ultrasound, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, P. R. China.

Background: The COVID-19 pandemic has become a new challenge to the medical system in various countries. The patients with ST-segment elevated myocardial infarction (STEMI) were also affected.

Methods: We used a random-effects mode to analyze the differences of the baseline characteristics and therapeutic features between STEMI patients admitted before and after the start of the COVID-19 pandemic.

Results: Thirty eight studies involving 79,753 patients were included in this analysis. The number of hospitalized STEMI patients decreased by 26% after the start of the COVID-19 pandemic. There were no differences in age, sex, prevalence of diabetes, hypertension, dyslipidemia or percutaneous coronary intervention rate between the STEMI patients before and after the start of the COVID-19 pandemic. However, the STEMI patients admitted after the start of the COVID-19 pandemic had a significantly increased time from symptom onset to first medical contact (standard mean difference: 0.51, 95% confidence interval: 0.24-0.78,  < .001) and an increased in-hospital mortality (odds ratio: 1.70, 95% confidence interval:1.14-2.56,  < .001); The in-hospital mortality of the STEMI patients with COVID-19 was 24% (95% confidence interval: 0.15-0.33); The in-hospital mortality of the STEMI patients with COVID-19 was significantly higher than that of the STEMI patients without COVID-19 at the initial stage of the COVID-19 pandemic (odds ratio: 7.28, 95% confidence interval: 2.75-19.28,  < .001).

Conclusion: The number of admitted STEMI patients was reduced while the in-hospital mortality and the time from symptom onset to first medical contact were increased during the COVID-19 pandemic.
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http://dx.doi.org/10.1080/23744235.2021.1953131DOI Listing
July 2021

Non-asthmatic eosinophilic bronchitis is characterized by proximal airway eosinophilic inflammation as compared with classic asthma and cough variant asthma.

Clin Exp Allergy 2021 Jul 26. Epub 2021 Jul 26.

State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University.

Non-asthmatic eosinophilic bronchitis (NAEB) is a common cause of chronic cough . NAEB involves eosinophilic airway inflammation, which manifests as sputum eosinophilia similar to that in cough variant asthma (CVA), in which cough presents as the sole manifestation, or in classic asthma (CA) which is characterized by wheezing, dyspnea and cough . However, NAEB lacks the airway hyperresponsiveness associated with asthma. The mechanism underlying these different clinical features remains unclear. A few studies have investigated pathological changes in the airways of patients with NAEB . A higher degree of mast cell infiltration was showed in the airway smooth muscle of asthmatic patients than that of NAEB , indicating that the site and degree of inflammatory cell infiltration may differ between NAEB and asthma. However, biopsies in previous studies were taken at a single site within the airway, and this may have prevented a full view of the differences in both the proximal airway and the periphery airway. Whether the degree of inflammation at different sites within the airway were different between NAEB and asthma remains unknown.
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http://dx.doi.org/10.1111/cea.13992DOI Listing
July 2021

Anaerobic phenanthrene biodegradation by a newly isolated sulfate-reducer, strain PheS1, and exploration of the biotransformation pathway.

Sci Total Environ 2021 Jul 20;797:149148. Epub 2021 Jul 20.

State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China. Electronic address:

Phenanthrene is a widespread and harmful polycyclic aromatic hydrocarbon that is difficult to anaerobically biodegrade. Current challenges in anaerobic phenanthrene bioremediation are a lack of degrading cultures and limited knowledge of biotransformation pathways. Under sulfate-reducing conditions, pure-cultures and biotransformation processes for anaerobic phenanthrene biodegradation are poorly understood. In this study, strain PheS1, which is phylogenetically closely related to Desulfotomaculum, was found to be a sulfate-reducing phenanthrene-degrading bacterium. Anaerobic phenanthrene biodegradation using PheS1 was proposed based on metabolite and genome analyses, and the initial step was identified as carboxylation based on the detection of 2-phenanthroic acid, [C]-2-phenanthroic acid, and [D9]-2- phenanthroic acid when phenanthrene+HCO, phenanthrene+HCO, and [D10]-phenanthrene+HCO were used as the substrate, respectively. PheS1 genome ubiD gene encoding of carboxylase putatively involved in the biodegradation was performed. Next, benzene ring reduction and cleavage that produced benzene compounds and cyclohexane derivative were reported to occur in the downstream biotransformation processes. Additionally, benzene, naphthalene, benz[a]anthracene, and anthracene can be utilised by PheS1, whereas pyrene and benz[a]pyrene cannot. We discovered a new phenanthrene-degrading sulfate-reducer and provided the anaerobic phenanthrene biotransformation pathway under sulfate-reducing conditions, which can act as a reference for practical applications in bioremediation and for studying the molecular mechanisms of phenanthrene in anaerobic zones.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149148DOI Listing
July 2021

What and Where: Learn to Plug Adapters via NAS for Multidomain Learning.

IEEE Trans Neural Netw Learn Syst 2021 Jul 26;PP. Epub 2021 Jul 26.

As an important and challenging problem, multidomain learning (MDL) typically seeks a set of effective lightweight domain-specific adapter modules plugged into a common domain-agnostic network. Usually, existing ways of adapter plugging and structure design are handcrafted and fixed for all domains before model learning, resulting in learning inflexibility and computational intensiveness. With this motivation, we propose to learn a data-driven adapter plugging strategy with neural architecture search (NAS), which automatically determines where to plug for those adapter modules. Furthermore, we propose an NAS-adapter module for adapter structure design in an NAS-driven learning scheme, which automatically discovers effective adapter module structures for different domains. Experimental results demonstrate the effectiveness of our MDL model against existing approaches under the conditions of comparable performance.
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http://dx.doi.org/10.1109/TNNLS.2021.3082316DOI Listing
July 2021

Clinical observation of octreotide combined with diclofenac sodium in preventing ERCP-related pancreatitis.

Am J Transl Res 2021 15;13(6):7179-7185. Epub 2021 Jun 15.

Department of Gastrointestinal Surgery, Nanhua Hospital, Nanhua University Hengyang, Hunan Province, China.

Objective: To elucidate the efficacy of octreotide in combination with diclofenac sodium in the prevention of ERCP-related pancreatitis, and investigate its impact on patients' serum amylase, white blood cell (WBC) count, adverse effects, hyperamylasemia and hemorheology.

Methods: The prospective study was conducted, in which 124 patients who underwent endoscopic retrograde cholangiopancreatography (ERCP) in our hospital were evenly divided into 2 groups, the observation group (n=62) and the control group (n=62), via a random number table method. The control group was administered diclofenac sodium lidocaine hydrochloride via injection after ERCP, while the observation group was given octreotide acetate on the basis of the control group. The incidence of pancreatitis, serum amylase level, WBC count, incidence of adverse effects and hyperamylasemia, and hemorheology levels were compared between these two groups of patients.

Results: The incidence of pancreatitis in the observation group was significantly lower than that in the control group (P<0.05). After treatment, the serum amylase level at 24 h post-surgery in the observation group was notably lower than that in the control group (P<0.001). The WBC count at 2 h and 24 h post-surgery in the observation group were both significantly lower than those in the control group (all P<0.001). The incidence of total adverse reactions in the observation group was remarkably lower than that in the control group (P<0.01). The incidence of hyperamylasemia in the observation group was considerably lower than that in the control group (P<0.001). Twenty-four hours post-surgery, the whole blood viscosity at high shear rate, whole blood viscosity at low shear rate, and plasma viscosity in the observation group were all significantly lower than those in the control group (all P<0.001).

Conclusion: The combination of octreotide and diclofenac sodium could effectively prevent the occurrence of ERCP-related pancreatitis, which reduced the incidence of hyperamylasemia, decreased the WBC count and serum amylase level, improved the hemorheology, and lowered the incidence of adverse effects in patients after ERCP. Therefore, this therapeutic strategy is worthy of clinical propagation and application.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290821PMC
June 2021

circMYC promotes cell proliferation, metastasis, and glycolysis in cervical cancer by up-regulating MET and sponging miR-577.

Am J Transl Res 2021 15;13(6):6043-6054. Epub 2021 Jun 15.

Department of Radiation Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer Tianjin, China.

Objective: To analyze the role of circMYC in cervical cancer.

Methods: Protein and RNA expression was detected by RT-qPCR and western blotting. Transwell, CCK8, and colony formation assays were used for measuring metastasis, cell viability, and proliferation, respectively. Lactate production, glucose uptake, and ATP generation were examined to evaluate cell glycolysis. Interactions between circMYC, miR-577, and MET were determined by RNA pull-down and immunoprecipitation, and dual-luciferase reporter assays. Xenografts were established in mice to evaluate the functions of circMYC .

Results: circMYC was overexpressed in tumor tissue, which was related to poor prognosis. CircMYC knockdown reduced proliferation, colony formation, metastasis, and glycolysis in cervical cancer cells as well as inhibiting tumor growth . Mechanistically, circMYC targeted miR-577, and the effects of circMYC knockdown could be reversed by miR-577 inhibition. Moreover, miR-577 downregulated the expression of MET. Therefore, the oncogenic role of circMYC in cervical cancer was achieved by sponging miR-577 and maintaining MET expression.

Conclusion: circMYC promotes cervical cancer progression through regulation of the miR-577/MET axis. circMYC may thus be a potential target for diagnosing and treating cervical cancer.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290748PMC
June 2021

GGC Repeat Expansion in the Gene Is Associated With a Phenotype of Predominant Motor-Sensory and Autonomic Neuropathy.

Front Genet 2021 7;12:694790. Epub 2021 Jul 7.

Department of Neurology, Peking University First Hospital, Beijing, China.

There is still a considerable proportion of patients with inherited peripheral neuropathy (IPN) whose pathogenic genes are unknown. This study was intended to investigate whether the GGC repeat expansion in the is presented in some patients with IPN. A total of 142 unrelated mainland Chinese patients with highly suspected diagnosis of IPN without any known causative gene were recruited. Repeat-primed polymerase chain reaction (RP-PCR) was performed to screen GGC repeat expansion in , followed by fluorescence amplicon length analysis-PCR (AL-PCR) to determine the GGC repeat size. Detailed clinical data as well as nerve, muscle, and skin biopsy were reviewed and analyzed in the -related IPN patients. In total, five of the 142 patients (3.52%) were found to have pathogenic GGC expansion in , with repeat size ranging from 126 to 206 repeats. All the -related IPN patients presented with adult-onset motor-sensory and autonomic neuropathy that predominantly affected the motor component of peripheral nerves. While tremor and irritating dry cough were noted in four-fifths of the patients, no other signs of the central nervous system were presented. Electrophysiological studies revealed both demyelinating and axonal changes of polyneuropathy that were more severe in lower limbs and asymmetrically in upper limbs. Sural nerve pathology was characterized by multiple fibers with thin myelination, indicating a predominant demyelinating process. Muscle pathology was consistent with neuropathic changes. P62-positive intranuclear inclusions were observed in nerve, skin, and muscle tissues. Our study has demonstrated that GGC expansion in is associated with IPN presenting as predominant motor-sensory and autonomic neuropathy, which expands the phenotype of the -related repeat expansion spectrum. Screening of GGC repeat expansions in the should be considered in patients presenting with peripheral neuropathy with tremor and irritating dry cough.
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http://dx.doi.org/10.3389/fgene.2021.694790DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293674PMC
July 2021

The regulation of 1-methylcyclopropene treatment on the subfamily genes expression of ethylene response factors in peaches during storage.

Acta Sci Pol Technol Aliment 2021 Jul-Sep;20(3):313-323

College of Food Science and Technology, Nanjing Agricultural University, Jiangsu, PR China.

Background: Ethylene response factors (ERFs) perform diverse functions in fruit development, ripening and senescence. However, the effects of postharvest treatments on ERF genes have not been widely investigated due to the lack of peach ERF genomic information. The aim of this study was to investigate the ERF genes' expression of freshly harvested peach during storage after 1-methylcyclopropene (1-MCP) treatment.

Methods: 10 μL L-1 1-MCP was used to fumigate peaches. Treated peaches and control peaches were stored at 20°C for 9 days. Fruit firmness, ethylene production and the transcript abundance of ERFs were evaluated during storage.

Results: 127 AP2/ERF genes were identified genome using RNA-sequencing (RNA-seq). Expression profiles of 39 ERF genes were considered at day 0, 3, 5 and 7. Results showed that 1-MCP inhibited some ERF genes' expression (e.g., Prupe.5G117800), some genes were generally up-regulated responding to 1-MCP (e.g., Prupe.6G039700), while the other ERF genes displayed no significant difference between the two groups (e.g., Prupe.1G130300).

Conclusions: These data revealed that peach ERF genes perform diverse functions during fruit growth, ripening and senescence. The different responses of ERF genes to postharvest 1-MCP treatment may be useful to understand the roles of ethylene and ERF genes in controlling technological aspects of postharvest peach conservation.
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http://dx.doi.org/10.17306/J.AFS.0943DOI Listing
July 2021

The unaddressed mental health burden among cancer patients in China: a call to action.

Lancet Psychiatry 2021 08;8(8):646-647

Department of Social and Behavioral Sciences, School of Global Public Health, New York University, New York, NY, USA; New York University Shanghai, Shanghai, China; Department of Health, Behavior and Society, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.

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http://dx.doi.org/10.1016/S2215-0366(21)00180-2DOI Listing
August 2021
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