Publications by authors named "Hui Li"

8,716 Publications

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Passivation of lead and cerium in soil facilitated by biochar-supported phosphate-doped ferrihydrite: Mechanisms and microbial community evolution.

J Hazard Mater 2022 May 6;436:129090. Epub 2022 May 6.

School of Resources & Environment, Northeast Agricultural University, Harbin150030, China. Electronic address:

The massive exploitation and application of heavy metals and rare earth elements (REEs) lead to their exceeding the standard in soil. Herein, a new type of biochar supported phosphorus doped ferrihydrite ([email protected]) has been designed and enhance passivation of Pb and Ce in soil. SEM images of [email protected] showed P-FH nanoparticles adhered to the natural cavity and large pore diameter on the surface of biochar, which greatly avoided the agglomeration of nanoparticles. The residual state of lead or cerium increased 161.4% or 43.9% by adding 3% [email protected] after 90 days of incubation in 500 mg/kg lead or cerium simulated contaminated soil. The passivation of cerium by [email protected] is obviously inhibited with the coexistence of lead. The results of [email protected] magnetically separated from the soil characterization indicate that complexation, co-precipitation and the formation of secondary minerals mainly contribute to the high efficiency passivation ability of [email protected] for lead and cerium. By changing the addition of [email protected], the soil pH can be adjusted and the soil organic matter and P contents can be improved. Moreover, [email protected] is an environmentally friendly material without ecotoxicity. And bacterial richness and diversity in soil were improved after passivation of Pb and Ce by adding [email protected]
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http://dx.doi.org/10.1016/j.jhazmat.2022.129090DOI Listing
May 2022

Construction of Pd Single Site Anchored on Nitrogen-Doped Porous Carbon and Its Application for Total Antioxidant Level Detection.

Nanoscale Res Lett 2022 May 20;17(1):54. Epub 2022 May 20.

School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, People's Republic of China.

Natural enzymes have excellent catalytic activity. However, due to their unstable nature and high cost, current research has turned to the synthesis and development of enzyme-like nanomaterials and single-atomic nanozymes. In this study, a single-atomic palladium-loaded nitrogen-doped porous carbon catalyst (SA-Pd/NPC) was prepared and used as a mimetic peroxidase to catalyze the substrates oxidation. The catalytic capability of the SA-Pd/NPC was tested by the TMB-HO system, and it expressed a superior catalytic capability owing to the plentiful catalytic centers of the single-atom Pd, its high porosity, the large specific surface area, and the strong electron transfer capability of the NPC. For the color reaction of TMB, thiol antioxidants (e.g., glutathione, GSH) and non-thiol antioxidants (e.g., ascorbic acid, AA) are suitable for different inhibition mechanisms. GSH and AA are typical substances of these two main antioxidant types, respectively. Here, we demonstrate that this prepared catalyst could be used to simultaneously determine a variety of major known physiologically relevant thiol-containing and thiol-free antioxidants, accompanied by a blue color gradient change with UV-Vis spectra at 652 nm through the SA-Pd/NPC-catalyzed TMB-HO system. Linear responses to GSH and AA could be obtained in the concentration ranges of 0.01-0.10 mM and 1-13 μM (both R values were greater than 0.970), respectively, while the limits of detection were 3 μM and 0.3 μM, respectively. The ability of the nanozyme to detect overall antioxidant levels (TAL) was also confirmed in subsequent tests on artificial saliva and biological samples.
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http://dx.doi.org/10.1186/s11671-022-03693-5DOI Listing
May 2022

Lactic acid bacteria isolated from Kazakh traditional fermented milk products affect the fermentation characteristics and sensory qualities of yogurt.

Food Sci Nutr 2022 May 19;10(5):1451-1460. Epub 2022 Apr 19.

China-Australia Joint Research Center for Dairy Future Technology Beijing Key Laboratory of Nutrition, Health & Food Safety Beijing Engineering Laboratory for Geriatric Nutrition Food Research COFCO Nutrition & Health Research Institute Beijing China.

Lactic acid bacteria (LAB) play a crucial role in the development of the taste, texture, and aroma of traditional fermented milk products. Five LABs from Kazakh traditionally prepared dairy products showed continuous subculture stability, as well as proper acidification and coagulation ability. They were identified as (1-5, 1-7), (1-19), and (1-12, 1-15). Their coagulation time and acidity values ranged from 5.97 to 12.78 h and 76.47 to 89.39°T. Yogurts prepared with were more condensed and textural integrity than those with and . Determination of the volatile compound profiles suggested a higher diversity of volatile compounds than the control. The sensory evaluation presented positive overall sensory quality scores for the yogurts prepared with 1-12 and 1-15. The results provide additional information regarding the contributions of native LABs to the unique flavor and sensory qualities of traditionally prepared milk products. They may help to select starters or adjunct starters for developing distinctive, traditional nomadic fermented milk to satisfy consumer demand and increase market acceptability.
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http://dx.doi.org/10.1002/fsn3.2755DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9094460PMC
May 2022

Mimicking Metabolic Disturbance in Establishing Animal Models of Heart Failure With Preserved Ejection Fraction.

Front Physiol 2022 3;13:879214. Epub 2022 May 3.

Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.

Heart failure (HF), the terminal state of different heart diseases, imposed a significant health care burden worldwide. It is the last battlefield in dealing with cardiovascular diseases. HF with preserved ejection fraction (HFpEF) is a type of HF in which the symptoms and signs of HF are mainly ascribed to diastolic dysfunction of left ventricle, whereas systolic function is normal or near-normal. Compared to HF with reduced ejection fraction (HFrEF), the diagnosis and treatment of HFpEF have made limited progress, partly due to the lack of suitable animal models for translational studies in the past. Given metabolic disturbance and inflammatory burden contribute to HFpEF pathogenesis, recent years have witnessed emerging studies focusing on construction of animal models with HFpEF phenotype by mimicking metabolic disorders. These models prefer to recapitulate the metabolic disorders and endothelial dysfunction, leading to the more detailed understanding of the entity. In this review, we summarize the currently available animal models of HFpEF with metabolic disorders, as well as their advantages and disadvantages as tools for translational studies.
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http://dx.doi.org/10.3389/fphys.2022.879214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110887PMC
May 2022

Influence of Solvent-Dependent Morphology on Molecular Doping and Charge Transport in Conductive Thiophene Polymer.

Materials (Basel) 2022 May 4;15(9). Epub 2022 May 4.

State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.

The utility of a solvent is one of the key factors that impacts resultant film morphology. However, the effect of solvent-dependent morphology on the doping process and electrical conductivity has not been adequately elucidated. In this work, we compared the morphology of chloroform- and chlorobenzene-processed thiophene polymer films and investigated how the choice of solvent influences film morphology, doping level, charge transport properties, and thus electrical conductivity. It was found that the film drop-casted from chloroform exhibits better crystallinity than that drop-casted from chlorobenzene. The crystallinity has negligible impact on the doping level but significant impact on charge transport properties. As a result, the chloroform-processed film shows a higher electrical conductivity of up to 408 S cm due to a high carrier mobility related to the continuously crystalline domains in film. This finding indicates that the choice of solvent for preparation of film, which strongly correlated with molecular orientation, is a new strategy to optimize the electrical conductivity of doped polymers.
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http://dx.doi.org/10.3390/ma15093293DOI Listing
May 2022

Individual Tree Species Classification Based on Convolutional Neural Networks and Multitemporal High-Resolution Remote Sensing Images.

Sensors (Basel) 2022 Apr 20;22(9). Epub 2022 Apr 20.

College of Geodesy and Geomatics, Shandong University of Science and Technology, Qingdao 266590, China.

The classification of individual tree species (ITS) is beneficial to forest management and protection. Previous studies in ITS classification that are primarily based on airborne LiDAR and aerial photographs have achieved the highest classification accuracies. However, because of the complex and high cost of data acquisition, it is difficult to apply ITS classification in the classification of large-area forests. High-resolution, satellite remote sensing data have abundant sources and significant application potential in ITS classification. Based on Worldview-3 and Google Earth images, convolutional neural network (CNN) models were employed to improve the classification accuracy of ITS by fully utilizing the feature information contained in different seasonal images. Among the three CNN models, DenseNet yielded better performances than ResNet and GoogLeNet. It offered an OA of 75.1% for seven tree species using only the WorldView-3 image and an OA of 78.1% using the combinations of WorldView-3 and autumn Google Earth images. The results indicated that Google Earth images with suitable temporal detail could be employed as auxiliary data to improve the classification accuracy.
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http://dx.doi.org/10.3390/s22093157DOI Listing
April 2022

Syndrome of inappropriate antidiuretic hormone secretion is associated with different proton pump inhibitor use: a pharmacovigilance study.

BMC Nephrol 2022 May 19;23(1):191. Epub 2022 May 19.

Research Group of Jian Gong on Pharmacoepidemiology and Clinical Drug Evaluation, School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, P.O.Box 88, No.103 Wenhua Road, Shenyang, 110016, P.R. China.

Aim: The objective of this study was to evaluate the reported associations between the syndrome of inappropriate antidiuretic hormone secretion (SIADH) and a variety of proton pump inhibitors (PPI) through analysis of the reports extracted from the Food and Drug Administration Adverse Event Reporting System (FAERS).

Methods: FAERS reports from January 2004 to March 2020 were used to conduct disproportionality and Bayesian analyses. The definition of SIADH relied on the preferred terms provided by the Medical Dictionary for Regulatory Activities. The time to onset, mortality, and hospitalization rates of PPI-related SIADH were also investigated.

Results: The study identified a total of 273 reports of PPI-associated SIADH, which appeared to influence more elderly than middle-aged patients (71.1% vs. 12.5%). Women were more affected than men (48.7% vs. 41.8%). Rabeprazole had a stronger SIADH association than other PPIs based on the highest reporting odds ratio (reporting odds ratio = 13.3, 95% confidence interval (CI) = 7.2, 24.9), proportional reporting ratio (proportional reporting ratio = 13.3, χ = 113.7), and empirical Bayes geometric mean (empirical Bayes geometric mean = 13.3, 95% CI = 7.9). The median time to SIADH onset was 22 (interquartile range 6-692) days after PPI administration. PPI-associated SIADH generally led to a 2.95% fatality rate and a 79.7% hospitalization rate. The highest hospitalization death rate occurred in esomeprazole (91.2%).

Conclusion: According to our findings, more attention should be paid to SIADH within the first several months after the administration of PPIs. For women older than 65 years, dexlansoprazole may reduce the incidence of PPI-associated SIADH. Nonetheless, larger epidemiological studies are suggested to verify this conclusion.
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http://dx.doi.org/10.1186/s12882-022-02818-3DOI Listing
May 2022

Finite element analysis of 3D-printed personalized titanium plates for mandibular angle fracture.

Comput Methods Biomech Biomed Engin 2022 May 19:1-12. Epub 2022 May 19.

Department of Oral and Maxillofacial Surgery, The Second Hospital of Shandong University, Jinan, Shandong Province, People's Republic of China.

This paper discussed the size of 3 D-printed personalized titanium plates that can gain maximum stability of mandibular fracture and minimize stress shielding through finite element analysis. A 3 D virtual model of mandible with mandibular angle fracture was created from the CT data of patient. 3 D-printed personalized titanium plates varying in length and thickness were designed, and finite element analysis was performed under different loading conditions and fracture healing periods. After that, the stress distribution and deformation of the mandible with gonial fracture could be observed, and the stress shielding rate could be obtained. Finally, SPSS21.0 was used for statistical analysis. The results of finite element analysis indicated that as the thickness of titanium plates and the healing time decreased, the maximum displacement increased, under a certain condition in which the pore size, the width, the hole distance and the bridge spacing were 2.0 mm, 4.0 mm, 6.0 mm, 12.0 mm, respectively. What's more, in this condition, the thicker the titanium plate and the shorter the healing time were, the higher the stress shielding was at central occlusion. When the thickness of the personalized 3 D-printed titanium plate was 1.0 mm, the maximum displacement tended to be stable and the stress shielding was minimized. It can not only improve the bone stability after tension band fixation, but also minimize the stress shielding, which is expected to expand the indications of tension band fixation.
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http://dx.doi.org/10.1080/10255842.2022.2047952DOI Listing
May 2022

Effect of Early Electroacupuncture Combined with Enhanced Recovery after Surgery (ERAS) on Pain Perception and Dysfunction in Patients after Total Knee Arthroplasty (TKA).

Biomed Res Int 2022 9;2022:6560816. Epub 2022 May 9.

Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, China.

Objective: A retrospective case-control study was performed to observe the effect and clinical significance of early electroacupuncture combined with enhanced recovery after surgery (ERAS) on pain perception and dysfunction after total knee arthroplasty (TKA).

Methods: About 100 patients who diagnosed with TKA from February 2019 to April 2021 were enrolled in our hospital. The patients were arbitrarily assigned into control group and study group. The former group was cured with electroacupuncture in the early stage, and the latter group was intervened on the basis of early electroacupuncture combined with the concept of ERAS. The curative effect, the time of getting out of bed for the first time after operation, the time of postoperative rehabilitation, postoperative rehabilitation cost, pain score and knee joint function score, range of motion (ROM) of knee joint, low shear of whole blood viscosity, plasma viscosity, fibrinogen level, and postoperative complications were compared.

Results: There exhibited no statistical difference in clinical data. In terms of the treatment effects, there were 27 cases of markedly effective, 22 cases of effective, and 1 case of ineffective in the study group, and the total effective rate was 98.00%; in the control group, 15 cases were markedly effective, 28 cases were effective, and 13 cases were ineffective, and the total effective rate was 86.00%. Compared to the control group, the total effective rate of the study group was higher ( < 0.05). And the first time to get out of bed and the postoperative rehabilitation time in the study group were lower. Compared to the control group (10113.42 ± 524.83) yuan, the postoperative rehabilitation cost in the study group (12401.71 ± 530.77) yuan was higher. In terms of the scores of VAS and HSS, there exhibited no remarkable difference before treatment ( > 0.05). After treatment, the VAS score lessened and the HSS score augmented the study group VAS score (1.76 ± 0.28); the score in the control group was lower compared to the control group (3.45 ± 0.36), and HSS scoring (83.48 ± 11.23) points higher compared to the control group (65.82 ± 10.44) points ( < 0.05). The ROM of knee joint augmented successively at the 1st, 2nd, 4th, and 8th week after treatment comparison between groups, the ROM of the knee joint in the study group at the 1st, 2nd, 4th, and 8th week was (49.47 ± 3.60)°, (64.38 ± 5.32)°, (86.93 ± 6.72)°, and (104.20 ± 9.11)°, is higher compared to the control group (46.53 ± 3.41)°, (61.52 ± 5.20)°, (78.42 ± 6.45)°, and (98.77 ± 8.67)° ( < 0.05). One day after operation, there exhibited no remarkable difference in whole blood viscosity low shear, plasma viscosity, and fibrinogen level ( > 0.05). However, there exhibited no remarkable difference in plasma viscosity and fibrinogen level at 1 day and 7 days after operation ( > 0.05). Seven days after operation, the whole blood viscosity, plasma viscosity, and fibrinogen in the study group were lower ( < 0.05). The probability of postoperative complications was compared. In the study group, there were 2 cases of limb swelling and pain, 1 case of joint stiffness, and no swelling and pain complicated with deep venous thrombosis, and the total incidence was 6.00%. In the control group, there were 5 cases of limb swelling and pain, 3 cases of joint stiffness, and 3 cases of swelling and pain complicated with deep venous thrombosis, with a total incidence of 22.00%. The incidence of adverse reactions in the study group was lower (  = 5.317 < 0.05).

Conclusion: Early electroacupuncture combined with ERAS is of positive significance to the patients after TKA, which can reduce the pain, enhance the function of the knee joint, and promote the ROM of the knee joint, and can effectively shorten the first time out of bed and postoperative rehabilitation time and reduce whole blood viscosity low shear, plasma viscosity, and fibrinogen level, but the overall rehabilitation cost is high, and clinical application should be combined with the actual situation of patients.
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http://dx.doi.org/10.1155/2022/6560816DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110174PMC
May 2022

Current Progress of Mitochondrial Genome Editing by CRISPR.

Front Physiol 2022 2;13:883459. Epub 2022 May 2.

Guangdong Engineering Research Center for Marine Algal Biotechnology, Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Longhua Innovation Institute for Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, China.

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http://dx.doi.org/10.3389/fphys.2022.883459DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9108280PMC
May 2022

Whole mitochondrial genome analysis of the Daur ethnic minority from Hulunbuir in the Inner Mongolia Autonomous Region of China.

BMC Ecol Evol 2022 May 18;22(1):66. Epub 2022 May 18.

Department of Radiology, The First Affiliated Hospital of Shantou University Medical College, Shantou, 515041, China.

Background: Mitochondrial DNA (mtDNA) variations are often associated with bioenergetics, disease, and speciation and can be used to track the history of women. Although advances in massively parallel sequencing (MPS) technology have greatly promoted our understanding of the population's history (especially genome-wide data and whole Y chromosome sequencing), the whole mtDNA sequence of many important groups has not been fully studied. In this study, we employed whole mitogenomes of 209 healthy and unrelated individuals from the Daur group, a Mongolic-speaking representative population of the indigenous groups in the Heilongjiang River basin (also known as the Amur River basin).

Results: The dataset presented 127 distinct mtDNA haplotypes, resulting in a haplotype diversity of 0.9933. Most of haplotypes were assigned to eastern Eurasian-specific lineages, such as D4 (19.62%), B4 (9.09%), D5 (7.66%) and M7 (4.78%). Population comparisons showed that the Daurians do have certain connections with the ancient populations in the Heilongjiang River basin but the matrilineal genetic composition of the Daur group was also greatly influenced by other non-Mongolic groups from neighboring areas.

Conclusions: Collectively, the whole mtDNA data generated in the present study will augment the existing mtDNA database. Our study provides genetic links between the Daur population and the aborigine peoples from Siberia and the Amur-Ussuri Region. But on the whole, compared with other Mongolic-speaking groups, the modern Daur population is closer to the East Asian ancestry group.
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http://dx.doi.org/10.1186/s12862-022-02019-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9118598PMC
May 2022

Regioselective Synthetic Approach to Higher Alkenes from Lower Alkenes with Sulfoxides in the Fe/HO System Direct Alkylation or Arylation of the Csp-H Bond on the C═C Bond of Alkenes.

J Org Chem 2022 May 18. Epub 2022 May 18.

College of Chemistry and Chemical Engineering, Advanced Catalytic Engineering Research Center of the Ministry of Education, Hunan University, Changsha 410082, China.

The regioselective synthetic approach to higher alkenes from lower alkenes by using sulfoxides as alkyl or aryl reagents in the Fe/HO system has been developed. This reaction realized direct alkylation or arylation of alkenes. In this reaction, sulfoxides afforded one Csp or Csp atom to the C═C bond of alkenes; one new Csp-Csp bond or Csp-Csp bond was formed. Nearly 40 products including di-, tri-, and tetra-substituted products were regioselectively synthesized. Both aliphatic and aromatic alkenes could participate in this reaction. Moreover, not only dimethyl sulfoxide but also three other sulfoxides can be applied to this reaction, including diethyl, dibenzyl, and diphenyl sulfoxide. The mechanism studies showed that this reaction may experience a coupling process radical addition-elimination and the Fe/HO system made the sulfoxides offered one alkyl or aryl radical to the C═C bond of alkenes.
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http://dx.doi.org/10.1021/acs.joc.2c00047DOI Listing
May 2022

Colony‑stimulating factor CSF2 mediates the phenotypic plasticity of small‑cell lung cancer by regulating the p‑STAT3/MYC pathway.

Oncol Rep 2022 Jul 18;48(1). Epub 2022 May 18.

Translational Cancer Research Lab, Jilin Cancer Hospital, Changchun, Jilin 130000, P.R. China.

Relapse and drug resistance are the main causes of mortality in patients with small‑cell lung cancer (SCLC). Intratumoral heterogeneity (ITH) is a key biological mechanism that leads to relapse and drug resistance. Phenotypic plasticity is an important factor that leads to ITH in SCLC, although its mechanisms and key regulatory factors remain to be elucidated. In the present study, cell proliferation and cell switch assay were measured using trypan blue. Alamar Blue was used to test drug sensitivity. Differential genes were screened by RNA sequencing. Reverse transcription‑quantitative PCR and western blotting were performed to assess the expressions of CSF2/p‑STAT3/MYC pathway related molecules, neuroendocrine (NE)/non‑neuroendocrine (non‑NE), transcription factors and drug‑related targets. The present study found that SCLC cell line NCI‑H69 exhibited adherent (H69A) and suspensive (H69S) phenotypes, which could switch back and forth. The two phenotypic cells had significant differences in cellular NE and non‑NE characteristics, drug sensitivity and expression of drug‑related targets. RNA sequencing showed that granulocyte‑macrophage colony‑stimulating factor [i.e., colony‑stimulating factor 2 (CSF2)] was the main differentially expressed gene between the two phenotypes and that H69A cells highly expressed CSF2. The inhibition of CSF2 promoted the transformation from H69A to H69S, increased drug sensitivity and NE marker expression and decreased the non‑NE marker expression in H69A. The STRING, Pathway Commons and Reactome databases showed a potential regulatory relationship between CSF2 and phosphorylated signal transducer and activator of transcription 3 (p‑STAT3)/MYC. p‑STAT3 and MYC expression was higher in H69A cells than in H69S cells and CSF2 silencing inhibited their expression. Taken together, these results indicated that CSF2 may regulate the phenotypic plasticity of SCLC through the phosphorylated STAT3/MYC pathway, thereby limiting the transformation between cell clones with different phenotypes and changing the sensitivity of specific cell clones to targeted drugs. Targeting CSF2 may be a potential therapeutic strategy to overcome drug resistance in SCLC treatment by influencing ITH.
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http://dx.doi.org/10.3892/or.2022.8333DOI Listing
July 2022

Genome-Based Analysis of Aspergillus niger Aggregate Species from China and Their Potential for Fumonisin B and Ochratoxin A Production.

Curr Microbiol 2022 May 17;79(7):193. Epub 2022 May 17.

China Center of Industrial Culture Collection (CICC), China National Research Institute of Food and Fermentation Industries Co., Ltd, Beijing, 100015, China.

Based on entire genome sequencing, this study focused on the classification of Aspergillus niger aggregation species and investigated their potential for fumonisin B (FB) and ochratoxin A (OTA) production. In the current study, 22 strains were used, namely 17 A. niger strains, four A. welwitschiae strains, and one A. lacticoffeatus (a synonym of A. niger) strain. Traditional multigene phylogenetic analysis, average nucleotide identity analysis (ANI), and the whole-genome single-nucleotide polymorphism (SNP) analyses were used to reconfirm the taxonomic status of A. niger, A. welwitschiae, and A. lacticoffeatus. The ability of A. niger to produce FB and OTA on five culture substrates was determined, and the association between FB and OTA gene clusters and toxin-producing abilities was explored. The results revealed that the ANI method could distinguish A. niger from A. welwitschiae, with an ANI value of < 98%. The SNP-based phylogenetic analysis suggested that A. niger and A. welwitschiae were two independent phylogenetic species. The ANI, SNP, and multigene phylogenetic analysis supported previous findings that A. lacticoffeatus was a synonymous species of A. niger. Aspergillus niger strains exhibited the varied potential of producing FB and OTA on different culture media. The A. niger genome sequence analysis revealed no significant difference in fumonisin gene clusters between FB-nonproducing isolates and FB-producing isolates, and the integrity of the ochratoxin biosynthesis genes cluster was clearly associated with OTA production. In conclusion, gene sequencing can be useful in assessing A. niger's ability to produce OTA, but it cannot reliably predict its ability to produce FB.
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http://dx.doi.org/10.1007/s00284-022-02876-8DOI Listing
May 2022

New insights into the immunologic role of oligodendrocyte lineage cells in demyelination diseases.

J Biomed Res 2022 Apr 28:1-10. Epub 2022 Apr 28.

Research Centre, the Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen 518107, China.

Oligodendrocyte lineage cells (OL-lineage cells) are a cell population that are crucial for mammalian central nervous system (CNS) myelination. OL-lineage cells go through developmental stages, initially differentiating into oligodendrocyte precursor cells (OPCs), before becoming immature oligodendrocytes, then mature oligodendrocytes (OLs). While the main function of cell lineage is in myelin formation, and increasing number of studies have turned to explore the immunological characteristics of these cells. Initially, these studies focused on discovering how OPCs and OLs are affected by the immune system, and then, how these immunological changes influence the myelination process. However, recent studies have uncovered another feature of OL-lineage cells in our immune systems. It would appear that OL-lineage cells also express immunological factors such as cytokines and chemokines in response to immune activation, and the expression of these factors changes under various pathologic conditions. Evidence suggests that OL-lineage cells actually modulate immune functions. Indeed, OL-lineage cells appear to play both "victim" and "agent" in the CNS which raises a number of questions. Here, we summarize immunologic changes in OL-lineage cells and their effects, as well as consider OL-lineage cell changes which influence immune cells under pathological conditions. We also describe some of the underlying mechanisms of these changes and their effects. Finally, we describe several studies which use OL-lineage cells as immunotherapeutic targets for demyelination diseases.
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http://dx.doi.org/10.7555/JBR.36.20220016DOI Listing
April 2022

Albendazole induces immunotherapy response by facilitating ubiquitin-mediated PD-L1 degradation.

J Immunother Cancer 2022 May;10(5)

Molecular Biology Research Center, Center for Medical Genetics, Hunan Province Key Laboratory of Basic and Applied Hematology, School of Life Sciences, Central South University, Changsha, Hunan, China

Background: Immune checkpoint inhibitors (ICIs) have been increasingly used in patients with various cancers and have shown efficient therapeutic outcomes. However, fewer than 40% of cases across multiple cancer types show a response to ICIs. Therefore, developing more efficient combinational approaches with ICIs and revealing the underlying mechanisms are important goals for achieving rapid clinical transformation and application.

Methods: The effects on antitumor immunity activity of albendazole (ABZ) and the synergistic effects of ABZ with CD73 blockade were investigated in the melanoma B16F10 and the Lewis lung cancer tumor-bearing immune-competent mice models. The mechanism of ABZ reducing PD-L1 protein level through suppressing UBQLN4 was identified and validated through immunoprecipitation-mass spectrometry and molecular methods. Bioinformatics and anti-PD-1 therapy melanoma patients samples analysis were used to assess the level of UBQLN4/PD-L1 in the therapeutic efficacy of anti-PD-1 therapy.

Results: ABZ induces CD8 T cell activity and subsequent immunotherapy response associated with suppression of PD-L1 protein level. Mechanistically, we revealed that ABZ promotes ubiquitin-mediated degradation of PD-L1 via suppressing UBQLN4, which was bound to PD-L1 and stabilized PD-L1 protein. Preclinically, genetic deletion or target inhibition of CD73 showed synergistic effects with ABZ treatment in the immune-competent mice models. Significantly, UBQLN4 and PD-L1 levels were higher in the tumor region of responders versus non-responders and correlated with better progression-free survival and overall survival in anti-PD-1 therapy melanoma patients.

Conclusions: Our findings revealed a previously unappreciated role of ABZ in antitumor immunity by inducing ubiquitin-mediated PD-L1 protein degradation, identified predictors for assessing the therapeutic efficacy of anti-PD-1 therapy, and provided novel therapeutic possibility by combination treatment of ABZ and CD73 blockade in cancers.
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http://dx.doi.org/10.1136/jitc-2021-003819DOI Listing
May 2022

Liver transplantation for metastatic non-resectable gastrointestinal stromal tumor after molecular targeted therapies: A case report.

Int J Surg Case Rep 2022 May 11;95:107185. Epub 2022 May 11.

Department of Pathology, Xiang'an Hospital of Xiamen University, Xiamen 361000, China; Organ Transplantation Institute of Xiamen University, Fujian Provincial Key Laboratory of Organ and Tissue Regeneration, School of Medicine, Xiamen University, Xiamen 361000, China. Electronic address:

Introduction And Importance: Metastatic GIST (gastrointestinal stromal tumor) is most commonly seen in the liver. Surgical resection and Imatinib administration are the preferred treatment for localized and potentially resectable GIST. However, it is still a matter of debate about the optimal therapeutic management for unresectable, liver-confined, metastatic GIST even after the administration of imatinib. The present case illustrates the possibility of LT surgery maybe for unresectable GIST.

Case Presentation: A 56-year-old man revealed clinical symptoms such as abdominal pain, eating little, fullness of abdomen, and fatigue. A 6.0 cm tumor in the fundus of the stomach was found by gastroscopy, which was confirmed to be GIST by pathological biopsy and molecular testing. Abdominal enhanced CT scanning showed multiple hepatic mass occupying synchronous. Then the patient initiated on targeted drug therapy of Imatinib (400 mg daily). A year later, a follow-up abdominal enhanced CT scanning showed that no tumor was found in the gastric fundus except the thickened gastric wall with poor dilatation, and the liver tumors were significantly smaller than before. When the patient showed symptoms of drug resistance, he was refered to our hospital for LT. The surgery was very successful, and the patient is disease-free and there is no evidence of recurrence until the paper was finished.

Discussion And Conclusion: Metastatic GIST to the whole liver is a rare clinical entity requiring unique considerations for treatment. Treatment based on LT might be the last resort therapy for unresectable, liver-confined, metastatic GIST in transplant oncology.
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http://dx.doi.org/10.1016/j.ijscr.2022.107185DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9112116PMC
May 2022

Nitrogen-Doped and Surface Functionalized CDs: Fluorescent Probe for Cellular Imaging and Environmental Sensing of ClO.

J Fluoresc 2022 May 16. Epub 2022 May 16.

College of Chemistry and Chemical Engineering, Xi'an University of Science and Technology, Xi'an, 710054, People's Republic of China.

A novel nitrogen doped and surface functionalized fluorescent CDs (T1) was synthesized by one-step and green hydrothermal method, which exhibits a satisfactory fluorescence quantum yield and a series of admirable features such as good aqueous solubility, narrow particle size distribution, resistance to photobleaching as well as excitation-dependent behavior. Benefitting from above merits, T1 can be employed to serve as an outstanding sensing platform for sensitive and accurate detection of ClO by remarkable fluorescence "on-off" process with rapid and anti-interference. More notably, the good biocompatibility and photostability can ensure enormous bioimaging potential and successful application of T1 in monitoring of exogenous ClO in MG-63 cells. Meanwhile, T1 can also be regarded as a filter paper sensor providing a convenient and efficient analyzing technology for monitoring of free residual chlorine in practical environmental samples. All these results demonstrate that there exists promising possibility for practical applications of T1 in bioimaging systems and environmental monitoring.
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http://dx.doi.org/10.1007/s10895-022-02952-zDOI Listing
May 2022

2D phosphorus carbide as promising anode materials for Na/K-ion batteries from first-principles study.

J Mol Model 2022 May 16;28(6):152. Epub 2022 May 16.

School of Materials Science and Engineering, Changchun University of Science and Technology, Changchun, 130022, China.

First-principles calculations based on density functional theory were used to investigate the electrochemical performance of monolayer γ-PC for Na- and K-ion batteries. Molecular dynamics simulations indicate that the monolayer γ-PC have the thermal and dynamic stability. A substantial charge transfer from the Na/K atoms to the γ-PC sheet enhances the electrical conductivity of γ-PC. The results show that the adsorption energies of Na and K are 1.53 eV and 2.04 eV, respectively, which are much higher than Na/K bulk cohesive energy and sufficiently ensure stability and safety. Additionally, the low diffusion barriers on γ-PC monolayer are 0.034 eV for Na and 0.027 eV for K, indicating excellent rate performance. The γ-PC sheet has a high theoretical capacity for both Na (519.9 mAh/g) and K (326.6 mAh/g) ion batteries, which can satisfy the requirement of energy storage devices to anode materials. Our results strongly suggest that 2D γ-PC monolayer is an exceedingly promising anode material for both NIBs and KIBs with high adsorption energies, high capacity, and low diffusion barriers.
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http://dx.doi.org/10.1007/s00894-022-05144-5DOI Listing
May 2022

Investigating the quasi-liquid layer on ice surfaces: a comparison of order parameters.

Phys Chem Chem Phys 2022 May 16. Epub 2022 May 16.

Department of Physics, King's College London, Strand, London WC2R 2LS, UK.

Ice surfaces are characterized by pre-melted quasi-liquid layers (QLLs), which mediate both crystal growth processes and interactions with external agents. Understanding QLLs at the molecular level is necessary to unravel the mechanisms of ice crystal formation. Computational studies of the QLLs heavily rely on the accuracy of the methods employed for identifying the local molecular environment and arrangements, discriminating between solid-like and liquid-like water molecules. Here we compare the results obtained using different order parameters to characterize the QLLs on hexagonal ice (Ih) and cubic ice (Ic) model surfaces investigated with molecular dynamics (MD) simulations in a range of temperatures. For the classification task, in addition to the traditional Steinhardt order parameters in different flavours, we select an entropy fingerprint and a deep learning neural network approach (DeepIce), which are conceptually different methodologies. We find that all the analysis methods give qualitatively similar trends for the behaviours of the QLLs on ice surfaces with temperature, with some subtle differences in the classification sensitivity limited to the solid-liquid interface. The thickness of QLLs on the ice surface increases gradually as the temperature increases. The trends of the QLL size and of the values of the order parameters as a function of temperature for the different facets may be linked to surface growth rates which, in turn, affect crystal morphologies at lower vapour pressure. The choice of the order parameter can be therefore informed by computational convenience except in cases where a very accurate determination of the liquid-solid interface is important.
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http://dx.doi.org/10.1039/d2cp00752eDOI Listing
May 2022

The Relationship Between Negative Life Events and Internet Addiction Disorder Among Adolescents and College Students in China: A Systematic Review and Meta-Analysis.

Front Psychiatry 2022 27;13:799128. Epub 2022 Apr 27.

School of Rehabilitation and Health Preservation, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Objective: Internet Addiction Disorder (IAD) has become a social problem. Literature suggests that negative life events can cause numerous problematic behaviors and part of them will result in IAD. However, there is a lack of evidence that elucidates the association between negative life events and IAD. Thereby, we performed a comprehensive analysis to further document the relationship between negative life events and IAD among adolescents and college students in China.

Methods: We searched ten electronic databases for relevant articles. We extracted correlation coefficient (r) values from each study and calculated 95% confidence interval (95% CI) after applying Fisher's . A random-effect or fixed-effect model was applied to analyze the data. Heterogeneity was examined using I statistics and the Cochran's Q statistics.

Results: A total of 31 studies were involved in this meta-analysis. Positive correlation was observed between negative life events and IAD. The subtype interpersonal relationship of negative life events was closely associationed with IAD.

Conclusion: There were significant positive association between negative life events and IAD. The findings can be used to guide IAD interventions.

Systematic Review Registration: [https://www.crd.york.ac.uk/prospero/#recordDetails].
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http://dx.doi.org/10.3389/fpsyt.2022.799128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9091506PMC
April 2022

The Potential of Pre-fermented Juice or Inoculants to Improve the Fermentation Quality of Mixed Silage of Agro-Residue and Lucerne.

Front Microbiol 2022 28;13:858546. Epub 2022 Apr 28.

Department of Grassland Science, College of Agronomy, Hunan Agricultural University, Changsha, China.

The objective of this study was to determine the effect of pre-fermented juice, , and . on chemical composition, fermentation, aerobic stability, dynamics of microbial community, and metabolic pathway of a mixture of lucerne, wheat bran (WB), and rice straw (RS). All mixtures were ensiled for 1, 3, 5, 7, 15, 30, and 45 days after treatment with uninoculated (control, C); . [LP, 1 × 10 cfu/g of fresh weight (FW)]; (LB, 1 × 10 cfu/g of FW); LP + LB (LPB, 1 × 10 cfu/g of FW of each inoculant); and pre-fermented juice (J; 2 × 10 cfu/g of FW). Four lactic acid bacteria (LAB) species from three genera were cultured from the pre-fermented juice, with being dominant. The inoculants increased lactic acid (LA), decreased pH and ammonia nitrogen (AN) compared to C silage at earlier stages of ensiling, and high dry matter (DM) and water-soluble carbohydrate (WSC) content in inoculated silages. Adding LPB increased the abundance of , , and , resulting in the lowest pH. Pre-fermented juice enriched , , , , and , mainly enhanced accumulation of acetic acid (AA) and LA, and decreased pH, crude protein losses, AN, and hemicellulose contents. and had a high abundance in LB-treated and J silages, respectively, inhibited undesirable bacteria, and improved aerobic stability with more than 16 days. In addition, the metabolic pathways changed with time and inoculants promoted global metabolism. In conclusion, inoculations altered bacterial succession and metabolic pathways in silage; LB and pre-fermented juice enhanced ensiling by promoting pH reductions, enhancing concentrations of LA and AA, and extending aerobic stability more than 16 days.
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http://dx.doi.org/10.3389/fmicb.2022.858546DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096938PMC
April 2022

Studies on Bioactive Components of Red Ginseng by UHPLC-MS and Its Effect on Lipid Metabolism of Type 2 Diabetes Mellitus.

Front Nutr 2022 27;9:865070. Epub 2022 Apr 27.

Changchun University of Chinese Medicine, Jilin Ginseng Academy, Changchun, China.

Objectives: Red ginseng is a processed product of C.A. Meyer, which is one of the widely used medicinal and edible herbs for the treatment of type 2 diabetes mellitus (T2DM). Ginsenosides are its main pharmacologically active ingredient. This study aims to clarify the material basis of total ginsenosides of red ginseng (RGW) and verify the activity of RGW in treating lipid metabolism disorders caused by T2DM.

Methods: An ultrahigh performance liquid chromatography coupled with quadrupole time of flight mass spectrometry (UHPLC-Q-TOF-MS) technology was applied to quantitatively analyze RGW. A T2DM rat model was established to verify the activity of RGW in treating lipid metabolism disorders caused by diabetes. First, the changes in diabetes-related parameters were observed, then the biochemical parameters of the rat serum and liver were measured, and finally, the pathological sections of the rat liver were observed, and the content of short-chain fatty acids in stools was measured. The activity of RGW was verified by fatty degenerated HepG2 cells.

Results: A total of 10 ginsenosides were identified and quantitatively analyzed in RGW. Experimental results demonstrated that RGW can improve lipid metabolism disorders. RGW significantly reduced the fasting blood glucose and TG and TC levels in T2DM rats, and hepatic steatosis was significantly ameliorated. experiments by RGW treatment also significantly attenuated lipid deposition in HepG2 cells. RGW upregulated the content of 5 short-chain fatty acids in rat stools, which are related to lipid oxidation and liver gluconeogenesis.

Conclusion: The total RGW were quantitatively analyzed by UHPLC-MS, and its effect on lipid metabolism of T2DM was studied. The experiment demonstrated that red ginseng can regulate lipid metabolism and improve lipid deposition, which provides a promising development for red ginseng as a functional food.
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http://dx.doi.org/10.3389/fnut.2022.865070DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9094573PMC
April 2022

Adenosine A2a Receptor Regulates Autophagy Flux and Apoptosis to Alleviate Ischemia-Reperfusion Injury the cAMP/PKA Signaling Pathway.

Front Cardiovasc Med 2022 29;9:755619. Epub 2022 Apr 29.

Department of Anesthesiology, Zhongnan Hospital of Wuhan University, Wuhan, China.

Exploring effective methods to lessen myocardial ischemia-reperfusion injury still has positive significance. The adenosine A2a receptor (A2aR) has played a crucial part in cardiac ischemia-reperfusion injury. Previous studies revealed that the adenosine A2a receptor regulated autophagy, but the specific mechanism in myocardial ischemia-reperfusion injury was still unclear. We established an ischemia-reperfusion model (30 min of ischemia and 2 h of reperfusion) and a model with oxygen-glucose deprivation for 6 h and reoxygenation for 18 h (OGDR) . The ischemia-reperfusion injury resulted in prolonged QTc interval, left ventricular systolic dysfunction, and myocardial infarction. model, we found that the OGDR-induced autophagosomes and apoptosis caused myocardial cell death, as evidenced by a significant increase in the generation of lactate dehydrogenase and creatine kinase-MB. Furthermore, overactivated autophagy with rapamycin showed an anti-apoptotic effect. The interaction between autophagy and apoptosis in myocardial ischemia-reperfusion injury was complex and variable. We discovered that the activation of adenosine A2a receptor could promote the expression of Bcl-2 to inhibit the levels of Beclin-1 and LC3II. The number of autophagosomes exceeded that of autolysosomes under OGDR, but the result reversed after A2aR activation. Activated A2aR with its agonist CGS21680 before reperfusion saved cellular survival through anti-apoptosis and anti-autophagy effect, thus improving ventricular contraction disorders, and visibly reducing myocardial infarction size. The myocardial protection of adenosine A2a receptor after ischemia may involve the cAMP-PKA signaling pathway and the interaction of Bcl-2-Beclin-1.
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http://dx.doi.org/10.3389/fcvm.2022.755619DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099415PMC
April 2022

Effects of NRF-1 and PGC-1α cooperation on HIF-1α and rat cardiomyocyte apoptosis under hypoxia.

Gene 2022 May 12:146565. Epub 2022 May 12.

School of Basic Medicine, Ningxia Medical University, 1160 Shengli South Street, Xingqing District, Yinchuan City, Ningxia Hui Autonomous Region, China. Electronic address:

Background: Hypoxia is a primary inducer of cardiomyocyte injury, its significant marker being hypoxia-induced cardiomyocyte apoptosis. Nuclear respiratory factor-1 (NRF-1) and hypoxia-inducible factor-1α (HIF-1α) are transcriptional regulatory elements implicated in multiple biological functions, including oxidative stress response. However, their roles in hypoxia-induced cardiomyocyte apoptosis remain unknown. The effect HIF-α, together with NRF-1, exerts on cardiomyocyte apoptosis also remains unclear.

Methods: We established a myocardial hypoxia model and investigated the effects of these proteins on the proliferation and apoptosis of rat cardiomyocytes (H9C2) under hypoxia. Further, we examined the association between NRF-1 and HIF-1α to improve the current understanding of NRF-1 anti-apoptotic mechanisms.

Results: The results show that NRF-1 and HIF-1α are important anti-apoptotic molecules in H9C2 cells under hypoxia, although their regulatory mechanisms differ. NRF-1 could bind to the promoter region of Hif1a and negatively regulate its expression. Additionally, HIF-1β exhibited competitive binding with NRF-1 and HIF-1α, demonstrating a synergism between NRF-1 and the peroxisome proliferator-activated receptor-gamma coactivator-1α.

Conclusion: These results indicate that cardiomyocytes can regulate different molecular patterns to tolerate hypoxia, providing a novel methodological framework for studying cardiomyocyte apoptosis under hypoxia.
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http://dx.doi.org/10.1016/j.gene.2022.146565DOI Listing
May 2022

NaCl salinity enhances tetracycline bioavailability to Escherichia coli on agar surfaces.

Chemosphere 2022 May 11;302:134921. Epub 2022 May 11.

Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI, 48824, United States. Electronic address:

Soil salinity is a worldwide problem and is damaging soil functions. Meanwhile, increasing amounts of anthropogenic antibiotics are discharged to agricultural soils. Little is known about how soil salinity (e.g., NaCl) could influence the bioavailability of antibiotics to bacteria. In this study, a tetracycline-responsive Escherichia coli bioreporter grew on the surfaces of agar microcosms at the same tetracycline concentration (200 μg/L), but various NaCl concentrations (0.5-19.2 g/L) with estimated osmotic potential of -0.18 to -1.80 MPa, and agar content (0.3%-5%) with estimated intrinsic permeability of 38 to 32,928 nm. These agar microcosms mimicked very fine textured soils with a range of NaCl salinity. Increasing agar content lowered the intrinsic permeability hence decreasing tetracycline bioavailability to E. coli, due likely to the reduced mass transfer of tetracycline via water flow. Intriguingly, tetracycline bioavailability increased with increasing NaCl concentration which caused the increase in osmotic stress. This is contradictory to the notion that osmotic stress reduces bacterial chemical uptake. Further analysis of E. coli membrane integrity demonstrated that the enhanced tetracycline bioavailability to bacteria could result from the compromised cell membranes and enhanced membrane permeability at higher NaCl salinity. Overall, this study suggests that high soil salinity (NaCl) may enhance the selection pressure exerted by antibiotics on bacteria.
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http://dx.doi.org/10.1016/j.chemosphere.2022.134921DOI Listing
May 2022

Changes in the intestinal microbiota of Pacific white shrimp (Litopenaeus vannamei) with different severities of Enterocytozoon hepatopenaei infection.

J Invertebr Pathol 2022 May 11;191:107763. Epub 2022 May 11.

Jiangsu Marine Fisheries Research Institute, Nantong 226007, China.

The intestinal microbiota of the Pacific white shrimp Litopenaeus vannamei during Enterocytozoon hepatopenaei (EHP) infection was investigated by 16S rRNA gene-based analysis. The results showed that bacterial diversity in the intestine of L. vannamei was high, but it decreased with increasing severity of EHP infection. The relative abundances of the phyla Planctomycetes, Actinobacteria and Acidobacteria decreased significantly with a decrease in body size or EHP infection severity (P < 0.05). The most abundant genera were Pseudomonas, Methylobacterium, Bradyrhizobium, Bacteroides, Vibrio, Prevotella and so on. In addition, the relative abundances of some bacteria, such as Pseudomonas, Bradyrhizobium, Bacteroides and Vibrio, increased significantly with a decrease in body size or EHP infection severity (P < 0.05). These findings suggest that changes in the intestinal microbiota occur depending on the severity of EHP infection.
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http://dx.doi.org/10.1016/j.jip.2022.107763DOI Listing
May 2022

Enabling Long Cycle Life and High Rate Iron Difluoride Based Lithium Batteries by In Situ Cathode Surface Modification.

Adv Sci (Weinh) 2022 May 14:e2201419. Epub 2022 May 14.

School of Materials Science and Engineering, Xiangtan University, Xiangtan, Hunan, 411105, P. R. China.

Metals fluorides (MFs) are potential conversion cathodes to replace commercial intercalation cathodes. However, the application of MFs is impeded by their poor electronic/ionic conductivity and severe decomposition of electrolyte. Here, a composite cathode of FeF and polymer-derived carbon (FeF @PDC) with excellent cycling performance is reported. The composite cathode is composed of nanorod-shaped FeF embedded in PDC matrix with excellent mechanical strength and electronic/ionic conductivity. The FeF @PDC enables a reversible capacity of 500 mAh g with a record long cycle lifetime of 1900 cycles. Remarkably, the FeF @PDC can be cycled at a record rate of 60 C with a reversible capacity of 107 mAh g after 500 cycles. Advanced electron microscopy reveals that the in situ formation of stable Fe O layers on the surface of FeF prevents the electrolyte decomposition and leaching of iron (Fe), thus enhancing the cyclability. The results provide a new understanding to FeF electrochemistry, and a strategy to radically improve the electrochemical performance of FeF cathode for lithium-ion battery applications.
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http://dx.doi.org/10.1002/advs.202201419DOI Listing
May 2022

Relationship between HER2 overexpression and long-term outcomes of early gastric cancer: a prospective observational study with a 6-year follow-up.

BMC Gastroenterol 2022 May 13;22(1):238. Epub 2022 May 13.

Department of Gastroenterology and Hepatology, First Medical Center of Chinese PLA General Hospital, Beijing, 100853, China.

Background: Studies have confirmed the prognostic value of the expression status of human epidermal growth factor receptor 2 (HER2) in advanced gastric cancer. However, its role in early gastric cancer (EGC) remains largely unknown. This study explored the association between HER2 overexpression and clinical outcomes of patients with EGC.

Methods: A total of 211 patients who had undergone endoscopic treatment for pN0 EGC were enrolled. The HER2 expression status was assessed using immunohistochemistry (IHC).

Results: The prevalence of HER2 overexpression was 14.2%. HER2 overexpression showed a significant correlation with tumor location (P = 0.033). Multivariate analysis showed that HER2 overexpression was significantly associated with an increased risk of tumor recurrence in pN0 EGC (hazard ratio [HR] = 3.97; 95% confidence interval [CI] 1.30-12.14; P = 0.016) but not overall survival (OS) or disease-specific survival (DSS). Of the included patients, age was associated with OS (HR = 1.11; 95% CI 1.04-1.18; P = 0.002], whereas lymphovascular invasion was significantly associated with poor DSS (HR = 33.66; 95% CI 3.05-371.25; P = 0.004).

Conclusion: This study shows that HER2 overexpression is significantly associated with tumor recurrence in pN0 EGC. Hence, Her2 testing at diagnosis is important and differential treatment and/or follow up strategies for patients with Her2 overexpression may merit future study.
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http://dx.doi.org/10.1186/s12876-022-02309-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102633PMC
May 2022

Comparative Transcriptome Analysis Reveals Molecular Insights in Overwintering Monochamus alternatus (Coleoptera: Cerambycidae).

J Insect Sci 2022 May;22(3)

Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, 210037, China.

Monochamus alternatus, the dominant vector of Bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae), has caused immense damage to forest resources. In China, this vector was native to the southern regions but has spread northward recently. To adapt to more challenging environments in the northern winter, M. alternatus has evolved an intricate strategy for overwintering, which remains largely unknown. Herein, we compared the transcriptome data of the overwintering and non-overwintering larvae of M. alternatus larvae to investigate the molecular mechanisms in overwintering. A total of 53.10 GB clean bases and 28, 245 unigenes were obtained by RNA-seq. Analysis of 2597 upregulated and 2429 downregulated unigenes, as well as the enrichment of DEGs showed that many genes and pathways were jointly involved in the overwintering period. Besides, the accuracy of the RNA-seq data was tested by using qPCR experiment involving 13 selected genes. The results revealed that the overwintering process relied largely on the energy allocation trade-off. Specifically, overwintering M. alternatus inhibited energy-intensive activities, such as growth and molting, detoxification, and trehalose transport, and the reserved energy was skewed towards the synthesis of antifreeze compounds and immune response to cope with the deleterious effects of winter.
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http://dx.doi.org/10.1093/jisesa/ieac025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105011PMC
May 2022
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