Publications by authors named "He Zhang"

847 Publications

Analysis, occurrence, toxicity and environmental health risks of synthetic phenolic antioxidants: A review.

Environ Res 2021 Jun 16:111531. Epub 2021 Jun 16.

Department of Chemistry, College of Sciences, Northeastern University, Shenyang, 110819, China; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 100049, China.

The continuous improvement of living standards is related to higher requirements for the freshness and taste of food. For example, synthetic phenolic antioxidants (SPAs) are added to fats and fried foods as food additives to minimize the oxidative rancidity of oils and fats. Hence, the global use of SPAs is increasing year by year. Dibutyl hydroxytoluene is one of the widely used SPAs, often in combination with butyl hydroxyanisole or gallate SPAs. The extensive use of these compounds makes them and their transformation products to be widespread in various environmental media, including indoor dust, wastewater, river water, sewage sludge, and sediment, as well as human samples, such as nails and urine, at concentrations varying from nanogram per gram (ng/g) to microgram per gram (μg/g). Animal experiments have shown that high-dose SPA exposure is toxic, which may lead to DNA damage and mismatches and the development of cancerous tumors. Since the biosphere shares the same set of genetic codes, humans and animals have many identical or similar feedback mechanisms and information pathways. Therefore, the damage of SPAs to animals may also threaten human health. This review discusses the properties, occurrence, analysis, and environmental health risks of typical SPAs, including butyl hydroxyanisole, dibutyl hydroxytoluene, tert-butylhydroquinone, propyl gallate, octyl gallate, and lauryl gallate, used as food additives. In addition, AO2246, which is used in food packaging bags, is also considered. Future research directions on SPAs and their transformation products (TPs) are identified and discussed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envres.2021.111531DOI Listing
June 2021

Ultrasound-mediated molecular self-assemble of thymol with 2-hydroxypropyl-β-cyclodextrin for fruit preservation.

Food Chem 2021 Jun 9;363:130327. Epub 2021 Jun 9.

Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, PR China; Horticultural Products Cold Chain Logistics Technology and Equipment National-Local Joint Engineering Laboratory, Hangzhou 310058, PR China; Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University, Hangzhou 310058, PR China. Electronic address:

In this study, the inclusion complex (IC) of thymol with 2-hydroxypropyl-β-cyclodextrin (HPβCD) was fast synthetized by ultrasonic technology and its antifungal activities were evaluated. The thymol/HPβCD-IC was characterized by UV-vis absorption spectroscopy, fluorescence emission spectroscopy, powder X-ray diffraction, FT-IR, H-NMR, TGA and DSC. The phase solubility studies proved that the aqueous solubility of thymol was significantly improved by forming the inclusion complex with HPβCD, and the thermal stability analysis showed that thymol/HPβCD-IC had a better thermal stability than pure thymol. The in vitro antifungal activities of thymol/HPβCD-IC against Botrytis cinerea, Penicillium digitatum and Alternaria alternata were significantly improved compared with pure thymol. Furthermore, the gray mold rot of tomatoes was evidently inhibited by thymol/HPβCD-IC treatment in vivo study. Therefore, the complexation with HPβCD assisted by ultrasound is a promising approach to solubilize and stabilize thymol for application as an antifungal agent in fruit preservation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2021.130327DOI Listing
June 2021

Nanozyme Sensor Array Plus Solvent-Mediated Signal Amplification Strategy for Ultrasensitive Ratiometric Fluorescence Detection of Exosomal Proteins and Cancer Identification.

Anal Chem 2021 Jun 18. Epub 2021 Jun 18.

Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.

Tumor exosomes with molecular marker-proteins inherited from their parent cells have emerged as a promising liquid biopsy biomarker for cancer diagnosis. However, facile, robust, and sensitive detection of exosomal proteins remains challenging. Therefore, a nanozyme sensor array is constructed by using aptamer-modified CN nanosheets (Apt/CN NSs) together with a solvent-mediated signal amplification strategy for ratiometric fluorescence detection of exosomal proteins. Three aptamers specific to exosomal proteins are selected to construct Apt/CN NSs for high specific recognition of exosomal proteins. The adsorption of aptamers enhances the catalytic activity of CN NSs as a nanozyme for oxidation of -phenylenediamine (PD) to 2,3-diaminophenazine (DAP). In the presence of target exosomes, the strong affinity between aptamer and exosome leads to the disintegration of Apt/CN NSs, resulting in a decrease of catalytic activity, thereby reducing the production of DAP. The ratiometric fluorescence signal based on a photoinduced electron transfer (PET) effect between DAP and CN NSs is dependent on the concentration of DAP generated, thus achieving highly facile and robust detection of exosomal proteins. Remarkably, the addition of organic solvent-1,4-dioxane can sensitize the luminescence of DAP without affecting the intrinsic fluorescence of CN NSs, achieving the amplification of the aptamer-exosome recognition events. The detection limit for exosome is 2.5 × 10 particles/mL. In addition, the accurate identification of cancer can be achieved by machine learning algorithms to analyze the difference of exosomal proteins from different patients' blood. We hope that this facile, robust, sensitive, and versatile nanozyme sensor array would become a promising tool in the field of cancer diagnosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.analchem.1c02010DOI Listing
June 2021

Combined application of rhamnolipid and agricultural wastes enhances PAHs degradation via increasing their bioavailability and changing microbial community in contaminated soil.

J Environ Manage 2021 Jun 11;294:112998. Epub 2021 Jun 11.

College of Natural Resources and Environment, Northwest A&F University, Yangling, 712100, Shaanxi, PR China; Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China, Ministry of Agriculture, Yangling, 712100, Shaanxi, PR China. Electronic address:

Either biosurfactants or agricultural wastes were frequently used to enhance degradation of PAHs in soil, but there is still not clear whether combined application of biosurfactants and agricultural wastes is more efficient. Rhamnolipid and/or agricultural wastes (mushroom substrate or maize straw) were mixed with PAHs-contaminated soil to explore their performances in the removal of PAHs. The present study showed that rhamnolipid combined with mushroom substrate (MR, 30.36%) or maize straw (YR, 30.76%) significantly enhanced the degradation of soil PAHs compared with single application of mushroom substrate (M, 25.53%) or maize straw (Y, 25.77%) or no addition (19.38%). The addition of agricultural wastes significantly (p < 0.001) enhanced concentration of dissolved organic carbon (DOC) in soil. The combined application obviously improved the bioavailability of PAHs in soils and exhibited synergistic effects on concentration of organic acid-soluble HMW PAHs and the degradation rate of total HMW PAHs. Meanwhile, the combined application significantly (p < 0.01) enhanced the abundance of dominant bacterial and fungal genera being connected with PAHs degradation. The removal rate of PAHs was positively correlated with the dominant genera of bacteria (r = 0.539-0.886, p < 0.05) and fungi (r = 0.526-0.867, p < 0.05) related to PAHs degradation. Overall, the combined application exhibited a better performance in the removal of PAHs in contaminated soil via increasing their bioavailability and changing microbial communities in soil.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jenvman.2021.112998DOI Listing
June 2021

Potent and Persistent Antibody Response in COVID-19 Recovered Patients.

Front Immunol 2021 28;12:659041. Epub 2021 May 28.

CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

SARS-CoV-2 has caused a global pandemic with millions infected and numerous fatalities. Virus-specific antibodies can be detected in infected patients approximately two weeks after symptom onset. In this study, we set up ELISA technology coating with purified SARS-CoV-2 S and N proteins to study the antibody response of 484 serum samples. We established a surrogate viral inhibition assay using SARS-CoV-2 S protein pseudovirus system to determine the neutralization potency of collected serum samples. Here, we report robust antibody responses to SARS-CoV-2 in 484 recovered patients varying from 154 to 193 days, with 92% of recovered patients displaying a positive virus-specific spike glycoprotein IgG (s-IgG) response, while the ratio of positive spike glycoprotein IgM (s-IgM) reached 63%. Furthermore, moderate to potent neutralization activities were also observed in 62% of patients, correlating significantly with s-IgG response. This study strongly supports the long-term presence of antibodies in recovered patients against SARS-CoV-2, although all serum samples were collected from individuals with mild or moderate symptoms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.659041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193946PMC
May 2021

Targeting Endothelial Cell-Specific Molecule 1 Protein in Cancer: A Promising Therapeutic Approach.

Front Oncol 2021 24;11:687120. Epub 2021 May 24.

Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Despite the dramatic advances in cancer research in the past few years, effective therapeutic strategies are urgently needed. Endothelial cell-specific molecule 1 (ESM-1), a soluble dermatan sulfate proteoglycan, also known as endocan, serves as a diagnostic and prognostic indicator due to its aberrant expression under pathological conditions, including cancer, sepsis, kidney diseases, and cardiovascular disease. Significantly, ESM-1 can promote cancer progression and metastasis through the regulation of tumor cell proliferation, migration, invasion, and drug resistant. In addition, ESM-1 is involved in the tumor microenvironment, containing inflammation, angiogenesis, and lymph angiogenesis. This article reviews the molecular and biological characteristics of ESM-1 in cancer, the underlying mechanisms, the currently clinical and pre-clinical applications, and potential therapeutic strategies. Herein, we propose that ESM-1 is a new therapeutic target for cancer therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2021.687120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8181400PMC
May 2021

An Antisense Circular RNA Regulates Expression of RuBisCO Small Subunit Genes in .

Front Plant Sci 2021 24;12:665014. Epub 2021 May 24.

Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan University, Haikou, China.

Circular RNA (circRNA) is a novel class of endogenous long non-coding RNA (lncRNA) and participates in diverse physiological process in plants. From the dataset obtained by high-throughput RNA sequencing, we identified a circRNA encoded by the sense strand of the exon regions spanning two RuBisCO small subunit genes, and , in . We further applied the single specific primer-polymerase chain reaction (PCR) and Sanger sequencing techniques to verify this circRNA and named it ( RNA ). Using quantitative real-time PCR (qRT-PCR), we found that shares a similar rhythmic expression pattern with other genes. The expression level of is 10-40 times lower than the expression levels of genes in the photosynthetic organs in , whereas the root lacked expression. Furthermore, we used the delaminated layered double hydroxide lactate nanosheets (LDH-lactate-NS) to deliver synthesized into seedlings. Our results indicate that could significantly depress the expression of . Given that was expressed at low concentration , we suggest that may represent a fine-tuning mechanism to regulating the expression of genes and protein content in .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2021.665014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8181130PMC
May 2021

Influence of Peanut, Sorghum, and Soil Salinity on Microbial Community Composition in Interspecific Interaction Zone.

Front Microbiol 2021 24;12:678250. Epub 2021 May 24.

College of Agronomy, Shenyang Agricultural University, Shenyang, China.

Soil microorganisms play important roles in crop production and sustainable agricultural management. However, soil conditions and crop selection are key determining factors for soil microbial communities. This study investigated the effect of plant types and soil salinity on the microbial community of interspecific interaction zone (II) based on the sorghum/peanut intercropping system. Microbial community diversity and composition were determined through PacBio single molecule, real-time sequencing of 16S rDNA and internal transcribed spacer (ITS) genes. Results showed Proteobacteria, Bacteroidota, and Acidobacteriota to be the dominant bacterial phyla in IP, II, and IS, whereas Ascomycota, Basidiomycota, and Mucoromycota were the dominant fungal phyla. Under salt-treated soil conditions, the plants-specific response altered the composition of the microbial community (diversity and abundance). Additionally, the interspecific interactions were also helpful for maintaining the stability and ecological functions of microbial communities by restructuring the otherwise stable core microbiome. The phylogenetic structure of the bacterial community was greatly similar between IP and II while that of the fungal community was greatly similar between IP and IS; however, the phylogenetic distance between IP and IS increased remarkably upon salinity stress. Overall, salinity was a dominant factor shaping the microbial community structure, although plants could also shape the rhizosphere microenvironment by host specificity when subjected to environmental stresses. In particular, peanut still exerted a greater influence on the microbial community of the interaction zone than sorghum.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2021.678250DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180576PMC
May 2021

Robust output-feedback torque controller design for series elastic actuators and its application in multi-level control frameworks.

ISA Trans 2021 May 31. Epub 2021 May 31.

State Key Laboratory of Robotics and System, Harbin Institute of Technology, Harbin 150001, China. Electronic address:

Robust output-feedback torque controller is developed for series elastic actuators (SEAs) in the presence of parameter uncertainties and external disturbances. The strong robustness of the proposed controller results from the filter-based observer which can estimate the velocity signals and the system lumped disturbance. The dynamic surface method is adopted to make the time-domain controller independent of any derivatives of the command reference, making the torque controller an ideal building block for multi-level control frameworks. The semiglobal stability of the closed-loop control system is proven under the assumption that only the state-independent uncertainty is bounded. The experimental results verify the effectiveness of the torque controller, and the implementation of two-level control frameworks, including the impedance control and SEA's load position control, further demonstrates its wide applicability.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.isatra.2021.05.035DOI Listing
May 2021

Abundance of Lactobacillus in porcine gut microbiota is closely related to immune response following PRRSV immunization.

Vet Microbiol 2021 May 26;259:109134. Epub 2021 May 26.

State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China. Electronic address:

Increasing evidence shows that gut microbiota plays a critical role in host immune system development and immune regulation, thus the composition of gut microbiota may affect how individuals respond to immunizations. Currently, little evidence is available on the correlation between porcine gut microbiota and vaccine immune response. Here, we investigated the influence of gut microbiota on immune response in pigs to porcine reproductive and respiratory syndrome virus (PRRSV) vaccine. Based on the antibody levels for PRRSV, the immunized pigs were divided into three groups (high, low, and others), and followed by virulent PRRSV challenge. The comprehensive analysis of microbial composition revealed that gut microbiota was similar in the richness and diversity among different groups before immunization. After immunization, the richness and diversity of gut microbial community in the high group were still similar to the low group, although there was a decrease in community diversity overtime. Interestingly, the antibody titer was positively correlated with the abundance of Lactobacillus in gut microbiota in immunized pigs. Further analysis indicated that gut microbial composition might be correlated to the clinical parameters such as body weight and rectal temperature after virus challenge. Taken together, our findings suggest that certain specific members of gut microbiota, such as Lactobacillus may serve as a mechanism for regulating the immune response following immunization in pigs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.vetmic.2021.109134DOI Listing
May 2021

Hepatitis C virus modulates signal peptide peptidase to alter host protein processing.

Proc Natl Acad Sci U S A 2021 Jun;118(22)

Institute for Advanced Co-Creation Studies, Osaka University, Osaka, 565-0871, Japan;

Immunoevasins are viral proteins that prevent antigen presentation on major histocompatibility complex (MHC) class I, thus evading host immune recognition. Hepatitis C virus (HCV) evades immune surveillance to induce chronic infection; however, how HCV-infected hepatocytes affect immune cells and evade immune recognition remains unclear. Herein, we demonstrate that HCV core protein functions as an immunoevasin. Its expression interfered with the maturation of MHC class I molecules catalyzed by the signal peptide peptidase (SPP) and induced their degradation via HMG-CoA reductase degradation 1 homolog, thereby impairing antigen presentation to CD8 T cells. The expression of MHC class I in the livers of HCV core transgenic mice and chronic hepatitis C patients was impaired but was restored in patients achieving sustained virological response. Finally, we show that the human cytomegalovirus US2 protein, possessing a transmembrane region structurally similar to the HCV core protein, targets SPP to impair MHC class I molecule expression. Thus, SPP represents a potential target for the impairment of MHC class I molecules by DNA and RNA viruses.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1073/pnas.2026184118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179148PMC
June 2021

MnO-graphene oxide hybrid nanomaterial with oxidase-like activity for ultrasensitive colorimetric detection of cancer cells.

Anal Bioanal Chem 2021 May 17. Epub 2021 May 17.

Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang, 110819, Liaoning, China.

Robust and sensitive cell-based enzyme-linked immunosorbent assay (CELISA) is of great significance in the diagnosis and screening of cancer. However, the method is limited by the high rate of negative results attributed to the instability of horseradish peroxidase (HRP), HO, and antibody. Here, we construct a folic acid-functionalized in situ-grown MnO nanosheet/graphene oxide hybrid (FA-MnO/GO) with oxidase-like activity instead of the anti-folate receptor antibody in traditional CELISA to resist the possible negative interference arising from unstable HRP, HO, and antibodies for more robust colorimetric detection of cancer cells. The functionalization of FA enables the selective binding between hybrid and cancer cells through the over-expressed folate receptor, and then the binding events are converted into quantitative colorimetric signals though the oxidation of the chromogenic substrate TMB catalyzed by MnO, allowing the detection of cancer cells with colorimetric method. Moreover, the construction of MnO/GO hybrid can synergistically enhance the oxidase-like activity of MnO and promote its dispersion in water, further ensuring the accuracy and sensitivity of the detection. A detection limit of 20 cancer cells is obtained by a plate reader, which is lower than those obtained by most reported CELISA methods for cancer cell detection, and as few as 75 cancer cells can be identified by the naked eye. This study not only provides a multifunctional sensing platform for robust and sensitive cancer cell detection, but also offers a promising oxidase-like mimic in the field of bioanalysis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00216-021-03399-0DOI Listing
May 2021

Retrospective surveillance of porcine circovirus 4 in pigs in Inner Mongolia, China, from 2016 to 2018.

Arch Virol 2021 Jul 13;166(7):1951-1959. Epub 2021 May 13.

Institute of Military Veterinary medicine, Academy of Military Medical Sciences, Changchun, 130122, China.

A novel circovirus designated "porcine circovirus type 4" (PCV4) was recently reported in pigs with severe clinical disease in Hunan Province, China. Relatively little is known about the molecular epidemiology of this recently discovered virus. In order to assess the prevalence of PCV4 infection in pigs and to analyze its genomic characteristics, 1683 clinical samples were collected in Inner Mongolia, China, from 2016 to 2018. The overall infection rate of PCV4 was 1.6% (27/1683) at the sample level and 21.6% (11/51) at the farm level, with rates ranging from 3.2% (1/31) to 20.0% (6/30) on different PCV4-positive pig farms. In addition, the PCV4 infection rates at both the sample and farm level increased from 2016 to 2018. This also showed that PCV4 was present in pigs in 2016 in China and therefore did not arrive later than this date. Additionally, our findings showed that PCV4 infections had no association with PCV2 or PCV3 infections. We sequenced the complete genomes of three PCV4 strains and found that the PCV4 strains had a high degree of genetic stability but shared less than 80% sequence identity with other circoviruses. We identified six amino acid mutations in the Rep protein and seven in the Cap protein. Phylogenetic analysis based on Cap and Rep sequences confirmed that the PCV4 strains grouped in an independent branch. Our findings provide important information about the prevalence and genetic characteristics of PCV4 strains.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00705-021-05088-wDOI Listing
July 2021

Plumbagin Inhibits Proliferation, Migration, and Invasion of Retinal Pigment Epithelial Cells Induced by FGF-2.

Tissue Cell 2021 Apr 28;72:101547. Epub 2021 Apr 28.

Department of Ophthalmology, The second Hospital of Hebei Medical University, Shijiazhuang, 05000, China. Electronic address:

Proliferative vitreoretinopathy (PVR) is a serious ophthalmic disease and characterized by the formation of proliferative membranes by retinal pigment epithelial (RPE) cells. In PVR, the contraction and traction of the fibrocellular membranes cause retinal detachment, which can cause reduction surgery for retinal detachment to fail. Fibroblast growth factor-2 (FGF-2) causes RPE cells to form extracellular matrix (ECM), promotes chemotaxis, mitosis, and positively promotes the disease process of PVR. Plumbagin (PLB) is a plant small molecule naphthoquinone compound. It has the functions in anti-tumor, anti-inflammatory, inhibit proliferation. We tried to investigate the possible effects of PLB on the biological behavior of ARPE-19 cells induced by FGF-2 and its underlying mechanisms. Our study confirmed that proliferation, migration, and invasion of ARPE-19 cells induced by FGF-2 (10 ng/ml) were significantly inhibited by PLB. PLB also significantly inhibits the expression of MMP-2/-9, collagen I Alpha 1 (Col1A1), collagen IV Alpha 1 (Col4A1), collagen VI Alpha 1 (Col6A1), and the phosphorylation of FGF receptor (FGFR)-1, FGFR-2, ERK, p38, JNK of FGF-2-induced ARPE-19 cells. In summary, PLB inhibits FGF-2-stimulated proliferation, migration, and invasion of ARPE-19 cells, which may take place through inhibiting the expression of MMP-2/-9, Col1A1, Col4A1, Col6A1, and the mitogen-activated protein kinase (MAPK) pathway. PLB may have a preventive effect on proliferation, migration, and invasion of FGF-2-induced ARPE-19 cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tice.2021.101547DOI Listing
April 2021

Identifying functioning and nonfunctioning adrenal tumors based on blood serum surface-enhanced Raman spectroscopy.

Anal Bioanal Chem 2021 May 8. Epub 2021 May 8.

Department of Urology, First Affiliated Hospital, China Medical University, Shenyang, 110001, Liaoning, China.

Adrenal tumors are common tumors in urology and they can be further divided into functioning and nonfunctioning tumors according to whether there is uncommon endocrine function. In clinical practice, the early identification and accurate assessment of adrenal tumors are essential for the guidance of subsequent treatment. However, a nonfunctioning adrenal tumor often lacks obvious clinical symptoms, making it difficult to be timely and precisely diagnosed by conventional examinations. Therefore, a rapid and accurate method for identifying the functioning and nonfunctioning adrenal tumors is urgently required to achieve precise treatment of adrenal tumors. In this study, surface-enhanced Raman spectroscopy was investigated as a diagnostic tool to identify the blood serum samples from healthy volunteers as well as the patients with functioning and nonfunctioning adrenal tumors. Based on the SERS peak analysis, abnormal glycolysis, DNA/RNA, and amino acid metabolites were found to be potential biomarkers for identifying patients with adrenal tumors, while metabolites related to disordered protein catabolism and excessive hormone secretion were expected to further differentiate functioning adrenal tumors from nonfunctioning adrenal tumors. In addition, principal component analysis followed by support vector machine (PCA-SVM) was further applied on those serum SERS measurements, and the classification accuracies of 96.8% and 84.5% were achieved for differentiating healthy group versus adrenal tumor group and functioning adrenal tumor group versus nonfunctioning adrenal tumor group, respectively. The results have demonstrated the prodigious potential of precise adrenal tumor diagnosis by using the blood serum surface-enhanced Raman spectroscopy technique.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00216-021-03381-wDOI Listing
May 2021

Hierarchical Density-Aware Dehazing Network.

IEEE Trans Cybern 2021 May 7;PP. Epub 2021 May 7.

The commonly used atmospheric model in image dehazing cannot hold in real cases. Although deep end-to-end networks were presented to solve this problem by disregarding the physical model, the transmission map in the atmospheric model contains significant haze density information, which cannot simply be ignored. In this article, we propose a novel hierarchical density-aware dehazing network, which consists of a the densely connected pyramid encoder, a density generator, and a Laplacian pyramid decoder. The proposed network incorporates density estimation but alleviates the constraint of the atmospheric model. The predicted haze density then guides the Laplacian pyramid decoder to generate a haze-free image in a coarse-to-fine fashion. In addition, we introduce a multiscale discriminator to preserve global and local consistency for dehazing. We conduct extensive experiments on natural and synthetic hazy images, which prove that the proposed model performs favorably against the state-of-the-art dehazing approaches.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TCYB.2021.3070310DOI Listing
May 2021

MicroRNA775 regulates intrinsic leaf size and reduces cell wall pectin levels by targeting a galactosyltransferase gene in Arabidopsis.

Plant Cell 2021 May;33(3):581-602

State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences and School of Advanced Agricultural Sciences, Peking University, Beijing 100871, China.

Plants possess unique primary cell walls made of complex polysaccharides that play critical roles in determining intrinsic cell and organ size. How genes responsible for synthesizing and modifying the polysaccharides in the cell wall are regulated by microRNAs (miRNAs) to control plant size remains largely unexplored. Here we identified 23 putative cell wall-related miRNAs, termed as CW-miRNAs, in Arabidopsis thaliana and characterized miR775 as an example. We showed that miR775 post-transcriptionally silences GALT9, which encodes an endomembrane-located galactosyltransferase belonging to the glycosyltransferase 31 family. Over-expression of miR775 and deletion of GALT9 led to significantly enlarged leaf-related organs, primarily due to increased cell size. Monosaccharide quantification, confocal Raman imaging, and immunolabeling combined with atomic force microscopy revealed that the MIR775A-GALT9 circuit modulates pectin levels and the elastic modulus of the cell wall. We also showed that MIR775A is directly repressed by the transcription factor ELONGATED HYPOCOTYL5 (HY5). Genetic analysis confirmed that HY5 is a negative regulator of leaf size that acts through the HY5-MIR775A-GALT9 repression cascade to control pectin levels. These findings demonstrate that miR775-regulated cell wall remodeling is an integral determinant of intrinsic leaf size in A. thaliana. Studying other CW-miRNAs would provide more insights into cell wall biology.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/plcell/koaa049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8136896PMC
May 2021

Synergistic activity of insulin combined with glucose on Toxoplasma gondii proliferation in Vero cells.

Chin Med J (Engl) 2021 May 4. Epub 2021 May 4.

Department of Immunology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan 450001, China Department of Pathology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, Henan 450008, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/CM9.0000000000001516DOI Listing
May 2021

Revealing the heating value characteristics of sludge-based hydrochar in hydrothermal process: from perspective of hydrolysate.

Water Res 2021 Jun 21;198:117170. Epub 2021 Apr 21.

Shanghai Institute for Design & Research on Environmental Engineering Co., Ltd; Shanghai Environmental Sanitation Engineering Design Institute Co., Ltd.

Hydrothermal treatment (HT) is a promising method to convert sewage sludge to hydrochar biofuel. The heating value is directly correlated to the carbon content in hydrochar; however, the release of organic matter from sludge to hydrolysate and the transfer of the Maillard reaction products generated in the hydrolysate to the solid phase alter the carbon content in hydrochar. In this study, the relationship between hydrolysate and heating value of sludge-based hydrochar was presented, aiming to explain how the calorific value of hydrochar was affected by HT conditions. We adopted a direct combustion test to verify its clean combustion features. Hydrochar derived at 260 °C and residence time of 4 h (HC 260-4) exhibited the highest calorific value (HHV = 26.23 MJ/kg) with an energy density of 1.43, and its fuel characteristics were similar to those of lignite. The increase in the HT temperature and residence time up to 260 °C and 4 h, respectively, was beneficial for enhancing HHV. Conversely, further increase of the HT temperature to 300 °C and HT time to 6 h yielded a decrease in HHV. Investigation of the underlying mechanism revealed that the protein and polysaccharide releasing from sludge to hydrolysate occurred the Maillard reaction (MR). The generated humic-like Maillard reaction product (MRP) was transferred to hydrochar, inducing an increase in the carbon content and calorific value and a decrease in the organic content of hydrolysate. As the carbohydrate content in the hydrolysate decreased, the MR was terminated, so no more MRP was transferred to hydrochar. At the same time, the protein was still continuously released at higher temperatures and longer residence times, yielding a decline in the HHV. Moreover, clean energy utilization was verified from the reduced nitrogen content in hydrochar and lower CO and NO emission of HC 260-4 in the combustion test. After the HT, increased hydrophobicity and a lower fraction of bound water improved the dewaterability, which is of great significance for applying hydrochar as biofuel. The findings of this study provided a new perspective to explain the heating value generation of hydrochar and more direct evidence to assess its clean combustion properties, with promising perspectives for practical applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.watres.2021.117170DOI Listing
June 2021

Insight Into Chromatin-Enriched RNA: A Key Chromatin Regulator in Tumors.

Front Cell Dev Biol 2021 14;9:649605. Epub 2021 Apr 14.

Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Science and Technology, Tongji University, Shanghai, China.

Chromatin-enriched RNAs (cheRNAs) constitute a special class of long noncoding RNAs (lncRNAs) that are enriched around chromatin and function to activate neighboring or distal gene transcription. Recent studies have shown that cheRNAs affect chromatin structure and gene expression by recruiting chromatin modifiers or acting as bridges between distal enhancers and promoters. The abnormal transcription of cheRNAs plays an important role in the occurrence of many diseases, particularly tumors. The critical effect of cancer stem cells (CSCs) on the formation and development of tumors is well known, but the function of cheRNAs in tumorigenesis, especially in CSC proliferation and stemness maintenance, is not yet fully understood. This review focuses on the mechanisms of cheRNAs in epigenetic regulation and chromatin conformation and discusses the way cheRNAs function in CSCs to deepen the understanding of tumorigenesis and provide novel insight to advance tumor-targeting therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2021.649605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079759PMC
April 2021

Beneficial Effects of Exogenous Melatonin on Overcoming Salt Stress in Sugar Beets ( L.).

Plants (Basel) 2021 Apr 28;10(5). Epub 2021 Apr 28.

College of Agriculture, Northeast Agricultural University, Harbin 150030, China.

Melatonin has been regarded as a promising substance that enhances the abiotic stress tolerance of plants. However, few studies have devoted attention to the role of melatonin in improving salt tolerance in sugar beets. Here, the effects of different application methods (foliar application (100 μM), root application (100 μM), and combined foliar and root application) of melatonin on the morphological and physiological traits of sugar beets exposed to salt stress were investigated. The results showed that melatonin improved the growth of sugar beet seedlings, root yield and sugar content, synthesis of chlorophyll, photosystem II (PS II) activity, and gas exchange parameters under salt stress conditions. Moreover, melatonin enhanced the capacity of osmotic adjustment by increasing the accumulation of osmolytes (betaine, proline, and soluble sugar). At the same time, melatonin increased the H-pump activities in the roots, thus promoting Na efflux and K influx, which maintained K/Na homeostasis and mitigated Na toxicity. In addition, melatonin strengthened the antioxidant defense system by enhancing the activities of antioxidant enzymes, modulating the ASA-GSH cycle, and mediating the phenylalanine pathway, which removed superoxide anions (O) and hydrogen peroxide (HO) and maintained cell membrane integrity. These positive effects were more pronounced when melatonin was applied by combined foliar and root application. To summarize, this study clarifies the potential roles of melatonin in mitigating salt stress in sugar beets by improving photosynthesis, water status, ion homeostasis, and the antioxidant defense system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/plants10050886DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146524PMC
April 2021

Fabrication and Property Regulation of Small-Size Polyamine Microcapsules via Integrating Microfluidic T-Junction and Interfacial Polymerization.

Materials (Basel) 2021 Apr 5;14(7). Epub 2021 Apr 5.

National Engineering Research Center of Novel Equipment for Polymer Processing, Key Laboratory of Polymer Processing Engineering (SCUT), Ministry of Education, South China University of Technology, Guangzhou 510641, China.

The self-healing system based on microencapsulated epoxy-amine chemistry is currently the self-healing system with the most practical application potential. It can be widely used in many epoxy-based materials with a size restriction for the microcapsules, such as fiber-reinforced composites, anti-corrosion coatings, etc. Although epoxy microcapsules of different sizes can be fabricated using different techniques, the preparation of polyamine microcapsules with suitable sizes and good performance is the prerequisite for further developing this self-healing system. In this investigation, based on the novel microencapsulation technique via integrating microfluidic T-junction and interfacial polymerization, the feasibility of preparing small-size polyamine microcapsules and the process regulation to optimize the properties of the small-size microcapsules were studied. We show that polyamine microcapsules with sizes smaller than 100 μm can be obtained through the T-junction selection and the feeding rate control of the polyamine. To regulate the small-size microcapsules' quality, the effects of the concentration of the shell-forming monomer and the solvent with different polarity in the reaction solution and the reaction condition were studied. It shows that dry, free-flowing small-size microcapsules can still be obtained when the shell-forming monomer concentration is higher and the solvent's polarity is lower, compared with the preparation of larger polyamine microcapsules. Although the change of reaction conditions (reaction temperature and duration) has a certain effect on the microcapsules' effective core content, it is relatively small. The results of this investigation further promote the potential application of the self-healing systems based on microencapsulated epoxy-amine chemistry in materials with a size restriction for the microcapsules.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ma14071800DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8038612PMC
April 2021

Interfacial Electron Engineering of Palladium and Molybdenum Carbide for Highly Efficient Oxygen Reduction.

J Am Chem Soc 2021 May 29;143(18):6933-6941. Epub 2021 Apr 29.

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.

Interfacial electron engineering between noble metal and transition metal carbide is identified as a powerful strategy to improve the intrinsic activity of electrocatalytic oxygen reduction reaction (ORR). However, this short-range effect and the huge structural differences make it a significant challenge to obtain the desired electrocatalyst with atomically thin noble metal layers. Here, we demonstrated the combinatorial strategies to fabricate the heterostructure electrocatalyst of MoC-coupled Pd atomic layers (AL-Pd/MoC) by precise control of metal-organic framework confinement and covalent interaction. Both atomic characterizations and density functional theory calculations uncovered that the strong electron effect imposed on Pd atomic layers has intensively regulated the electronic structures and d-band center and then optimized the reaction kinetics. Remarkably, AL-Pd/MoC showed the highest ORR electrochemical activity and stability, which delivered a mass activity of 2.055 A mg at 0.9 V, which is 22.1, 36.1, and 80.3 times higher than Pt/C, Pd/C, and Pd nanoparticles, respectively. The present work has developed a novel approach for atomically noble metal catalysts and provides new insights into interfacial electron regulation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/jacs.1c00656DOI Listing
May 2021

Enhancing Q-Switched Fiber Laser Performance Based on Reverse Saturable and Saturable Absorption Properties of CuCrO Nanoparticle-Polyimide Films.

ACS Appl Mater Interfaces 2021 May 29;13(18):21748-21755. Epub 2021 Apr 29.

State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, 2699 Qian-Jin Street, Changchun 130012, People's Republic of China.

We demonstrate CuCrO (CCO) nanoparticle (NP)-polyimide (PI) composite film as a saturable absorber (SA) to regulate the output characteristics of passively Q-switched fiber laser at 1.55 μm. Based on the reverse saturable and saturable absorptions of the CCO NP-PI film, the passively Q-switched fiber laser expressed two stages with the increase of pump power for substantial performance enhancement. Reverse saturation absorption is observed to introduce appropriate cavity loss, which constructs effective pathways for promoting both the modulation depth and over threshold degree, as well as reducing the photon lifetime. In particular, our results realized the pulse duration and repetition rate compressing simultaneously for the first time. The second stage output laser exhibits a peak power of 1016 mW and a single pulse energy of 183 nJ, which are about 88 and 9 times higher than those of the first stage. Furthermore, the optical-optical conversion efficiency is up to 1270%. All of these can evidently demonstrate the importance of the appropriate cavity loss design for optimizing the Q-switched pulse laser output characteristics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c02762DOI Listing
May 2021

Effects of Metal Ions on the Precipitation of Penta-O-galloyl-β-d-glucopyranose by Protein.

J Agric Food Chem 2021 May 25;69(17):5059-5066. Epub 2021 Apr 25.

Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, China.

In this study, the effects of metal ions (Al, Fe, Cu, and Zn) on precipitation of a purified gallotannin 1,2,3,4,6-penta-O-galloyl-β-d-glucopyranose (PGG) by bovine serum albumin (BSA) were quantitatively analyzed. The stoichiometric ratios of the complexation of metal ions to PGG and methyl gallate (MeG) which can be defined as gallotannins monomer were also explored. The results showed that the addition of metal ions could reduce the solubility of PGG-protein complex and increase the PGG-protein precipitation. Precipitation studies showed that Al and Fe with a higher stoichiometric ratio to PGG and MeG had greater effects on PGG-protein precipitation than Cu and Zn. The results of this study suggested that metal ions could combine with PGG to form PGG-metal complex and interact with protein to form PGG-metal-protein ternary complexes, which resulted in the increase of PGG-protein precipitation. Consequently, a model of interaction between metal ions and PGG-protein precipitation was proposed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.1c01185DOI Listing
May 2021

Clinical laboratory characteristics of patients with obstructive jaundice accompanied by dyslipidemia.

Clin Biochem 2021 Apr 21. Epub 2021 Apr 21.

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China. Electronic address:

Background: Abnormal lipid metabolism manifests as hypercholesterolemia in patients with obstructive jaundice due to lipoprotein X (LpX). Our aim was to explore the clinical laboratory characteristics of patients with obstructive jaundice accompanied by dyslipidemia in a large number of samples.

Methods: A total of 665 patients with obstructive jaundice were included and categorized into two groups (with/without dyslipidemia) based on the ratio of the sum of HDL-c and LDL-c to total cholesterol [(HDL-c + LDL-c)/TC] with a cut-off value of 0.695. Laboratory liver, kidney, and blood lipid parameters were determined. Cholesterol composition assessment was performed by ultracentrifugation and high-performance liquid chromatography (UC-HPLC), and serum protein profiles were analyzed by capillary electrophoresis.

Results: Liver function in patients with obstructive jaundice accompanied by dyslipidemia was more aggravated than that in patients with simple obstructive jaundice (P < 0.05). The (HDL-c + LDL-c)/TC ratio was negatively correlated with bilirubin levels (P < 0.05). In addition, the difference in ApoB/LDL-c ratios was statistically significant between the obstructive jaundice accompanied by dyslipidemia group and healthy control group (P < 0.05). The LDL-c concentration determined by the UC-HPLC method was more than five times that determined by the enzymatic method (P < 0.05). Bisalbuminemia was found in 43 of 60 patients with obstructive jaundice accompanied by hypercholesterolemia.

Conclusions: In patients with obstructive jaundice, the decreased (HDL-c + LDL-c)/TC ratio may be a novel marker to identify dyslipidemia secondary to LpX. The decreased ratio was associated with poor liver function and indicated disease progression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.clinbiochem.2021.04.017DOI Listing
April 2021

Predictive Factors for In-Hospital Preoperative Rupture in Hyperacute Type A Aortic Dissection.

Heart Surg Forum 2021 04 23;24(2):E379-E386. Epub 2021 Apr 23.

Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing China.

Objective: This study aims to figure out risk factors of in-hospital preoperative rupture of hyperacute type A aortic dissection (haTAAD) patients and build a prediction and risk stratification model.

Methods: From January 2011 to December 2019, 830 patients diagnosed as haTAAD from Nanjing Drum Tower Hospital were enrolled. Among them, 799 patients received prompt surgery and 31 suffered aortic rupture before operation. The association between in-hospital preoperative rupture and perioperative parameters were examined. Best subset selection was used for feature selection and ROC curve was used to identify the model.

Results: Age, winter season, back pain, preoperative hypotension, albumin and globulin ratio, high serum phosphorus level are risk factors for in-hospital preoperative rupture of haTAAD. On the basis of six variables with AUC 0.828, a nomogram was established. According to the robustness test, actual in-hospital preoperative ruptures were fitted well.

Conclusions: The in-hospital rupture prediction model was developed using logistic regression analysis. High serum phosphorus level is one of the strongest predictors. This nomogram may be useful when evaluating the risk of aortic dissection in-hospital rupture in future trials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1532/hsf.3765DOI Listing
April 2021

Advance of African swine fever virus in recent years.

Res Vet Sci 2021 May 14;136:535-539. Epub 2021 Apr 14.

College of Veterinary Medicine, Key Laboratory for Clinical Diagnosis and Treatment of Animal Diseases at the Ministry of Agriculture, Inner Mongolia Agricultural University, Inner Mongolia Autonomous Region, Huhhot 010018, People's Republic of China.

African swine fever (ASF) is one of the most devastating hemorrhagic infectious diseases that affect pigs and wild suids due to the lack of a vaccine or an effective treatment. The large dsDNA genome of African swine fever virus (ASFV) contains up to 167 ORFs that are predicted to encode proteins. Since its introduction to China in 2018, this genome has aroused the enthusiasm of researchers throughout the world. Here, we review the research progress on ASFV in recent years. Given the importance of this disease, this review will highlight recent discoveries in basic virology, focusing mainly on epidemiology, virulence, pathogenic mechanisms, diagnosis, vaccine development, and treatment; this will help in understanding virus-host interactions and disease prevention regarding ASFV.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.rvsc.2021.04.004DOI Listing
May 2021

Drug-resistant cancer cell-derived exosomal EphA2 promotes breast cancer metastasis via the EphA2-Ephrin A1 reverse signaling.

Cell Death Dis 2021 04 20;12(5):414. Epub 2021 Apr 20.

Public Laboratory, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, 300060, China.

Tumor metastasis induced by drug resistance is a major challenge in successful cancer treatment. Nevertheless, the mechanisms underlying the pro-invasive and metastatic ability of drug resistance remain elusive. Exosome-mediated intercellular communications between cancer cells and stromal cells in tumor microenvironment are required for cancer initiation and progression. Recent reports have shown that communications between cancer cells also promote tumor aggression. However, little attention has been regarded on this aspect. Herein, we demonstrated that drug-resistant cell-derived exosomes promoted the invasion of sensitive breast cancer cells. Quantitative proteomic analysis showed that EphA2 was rich in exosomes from drug-resistant cells. Exosomal EphA2 conferred the invasive/metastatic phenotype transfer from drug-resistant cells to sensitive cells. Moreover, exosomal EphA2 activated ERK1/2 signaling through the ligand Ephrin A1-dependent reverse pathway rather than the forward pathway, thereby promoting breast cancer progression. Our findings indicate the key functional role of exosomal EphA2 in the transmission of aggressive phenotype between cancer cells that do not rely on direct cell-cell contact. Our study also suggests that the increase of EphA2 in drug-resistant cell-derived exosomes may be an important mechanism of chemotherapy/drug resistance-induced breast cancer progression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41419-021-03692-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058342PMC
April 2021

Validation of claims-based indicators used to identify flare-ups in inflammatory bowel disease.

Therap Adv Gastroenterol 2021 31;14:17562848211004841. Epub 2021 Mar 31.

Department of Dermatology and Allergy, Herlev and Gentofte Hospital, University of Copenhagen, Hellerup, Denmark.

Background & Aims: There are currently no validated claims-based indicators for identifying a worsening of disease in patients with inflammatory bowel disease (IBD). Therefore, we aimed to develop and validate indicators that identify flare-ups of IBD using data from Danish nationwide registries.

Methods: Using Danish nationwide administrative data, we identified all patients with Crohn's disease (CD) or ulcerative colitis (UC) who had at least one measurement of faecal calprotectin between 1 January 2015 and 31 June 2017. We tested several different claims-based indicators of disease flare-ups against levels of faecal (F-)calprotectin (no flare-up: <250 mg/kg; mild flare-up: 250-1000 mg/kg; severe flare-up: ⩾1000 mg/kg). A generalised estimating equation was used to evaluate whether the proposed indicators could predict disease activity.

Results: A total of 890 children and 4719 adults with CD, and 592 children and 5467 adults with UC were included in the study. During the observation period, 48-61% and 48-55% of the CD and UC patients, respectively, had no flare-up, 26-29% (CD) and 24-26% (UC) experienced a mild flare-up, and 12-23% (CD) and 21-27% (UC) experienced a severe flare-up. Combinations of indicators that could predict a flare-up in CD and UC adults included hospitalisation, surgery, initiation or switch of biological therapy, treatment with systemic steroids, locally acting steroids or topical 5-aminosalicylates, colonoscopy/sigmoidoscopy, and magnetic resonance imaging/computed tomography. In children, only the number of gastroenterology visits was significant as an indicator among UC patients, and none were seen in children with CD. Overall, the indicator combinations resulted in a predictive ability of 0.62-0.67.

Conclusion: Administrative claims data can be useful for identifying patients exhibiting (F-calprotectin defined) flare-ups of their IBD. Clinically relevant events captured in the Danish national patient registry are associated with increased levels of calprotectin and hence increased disease activity, and can be used as valid outcomes in future studies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/17562848211004841DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020737PMC
March 2021