Publications by authors named "Filipe Modolo"

28 Publications

  • Page 1 of 1

Evaluation of two classification systems for oral epithelial dysplasia.

Oral Dis 2021 Apr 4. Epub 2021 Apr 4.

Department of Pathology, Health Sciences Center, Federal University of Santa Catarina, Florianopolis, Brazil.

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http://dx.doi.org/10.1111/odi.13867DOI Listing
April 2021

Anesthetic Efficacy of an Alternative Inferior Alveolar Nerve Block Technique Using an Extra-Short Needle: A Double-Blind Randomized Non-Inferiority Trial.

J Oral Maxillofac Surg 2021 May 30;79(5):1025.e1-1025.e8. Epub 2020 Dec 30.

Professor, Department of Dentistry, Federal University of Santa Catarina (UFSC), Florianópolis, Santa Catarina, Brazil.

Purpose: Inferior alveolar nerve block (IANB) is a frequently used anesthetic technique for restorative and surgical procedures in the mandible and its success rate usually ranges from 80 to 85%. Thus, this study aimed to compare the anesthetic efficacy of an alternative technique named superficial nerve block (SNB)-which consists of a modified approach using an extra-short needle inserted 10 mm deep and on a higher injection site to the conventional technique (CT) for IANB in healthy individuals.

Methods: About 20 participants received both SNB and CT with 1.8 mL of 2% lidocaine and 1:100,000 epinephrine. Thermal tests were performed 6 times, every 2 min, on the inferior canine and first molar to determine the success rate and anesthesia onset. Assessments were repeated at 20, 40, and 60 min to determine anesthesia duration. Sensitivity of lips and tongue was tested by clamp pressure. Pain perception during injection was assessed by a 100 mm visual analog scale (VAS).

Results: The success rate in accordance with anatomical location was molars (SNB = 90%; CT = 85%), canines (SNB = 25%; CT = 70%), lips (SNB = 45%; CT = 95%), and tongue (SNB = 85%; CT = 95%). Significant differences were found for canines (P = .012) and lips (P < .002). Moreover, median anesthesia onset was: molars (SNB = 6 min; CT = 4 min), canines (SNB = 6 min; CT = 6 min), lips (SNB = 10 min; CT = 6 min), and tongue (SNB = 8 min; CT = 4 min), whereas median duration was molars (SNB = 60 min; CT = 60 min), canines (SNB = 20 min; CT = 60 min), lips (SNB = 60 min; CT = 60 min), and tongue (SNB = 60 min; CT = 60 min). A significant difference was found for anesthesia onset on molars (P = .024) and lips (P = .009). Pain scores on VAS were SNB (median = 8.5 mm) and CT (median = 10.0 mm) (P = .398).

Conclusions: In healthy individuals, the anesthetic effects of SNB were noninferior to CT in molars and tongue, although efficacy was considerably inferior in anterior teeth and lips.
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http://dx.doi.org/10.1016/j.joms.2020.12.038DOI Listing
May 2021

Dystrophic calcification and respiratory metaplasia in the epithelial lining of odontogenic cysts: a study of 362 odontogenic cysts in a Brazilian population and literature review.

Pathol Res Pract 2020 Jun 18;216(6):152975. Epub 2020 Apr 18.

Department of Pathology, Federal University of Santa Catarina (UFSC), Florianopolis, Santa Catarina, Brazil. Electronic address:

Purpose: Main study: undertake a histological study of odontogenic cysts (OC) to determine the prevalence of dystrophic calcification and metaplasia to respiratory epithelium on a Brazilian population.

Literature Review: to review the literature for studies that investigated the prevalence of respiratory metaplasia and dystrophic calcification on OC.

Methods: Main study: a retrospective histopathological evaluation was made of the archives from a pathology laboratory. A total of 362 cases diagnosed as OC were identified; they were analyzed by two expert observers to determine the presence of dystrophic calcification and respiratory metaplasia. The association with sex, age and anatomic location was performed through statistical analysis.

Literature Review: a critical literature review was undertaken. Two main electronic databases (PubMed and LILACS) were searched. Retrospective studies of histological evaluation that determined the prevalence of epithelial metaplasia and dystrophic calcification on OC, with at least 10 cases, were included; their findings were summarized and discussed.

Results: Main study: the histological evaluation of OC revealed the presence of respiratory epithelium in 25 cases (6.9%) and dystrophic calcification in 24 cases (6.6%). Positive association was found to dystrophic calcification on residual cyst and age; respiratory metaplasia on OC and sex; respiratory metaplasia on residual cyst and gnatic bone; respiratory metaplasia in OC and gnatic bone; dystrophic calcification in OC and anatomic site of mandible.

Literature Review: eleven studies were included in the literature review, and respiratory metaplasia ranged from 0.0% to 19.2% while dystrophic calcification ranged from 2.5% to 40.5%.

Conclusions: the histological evaluation of this study found 6.9% of prevalence of respiratory metaplasia and 6.6% of dystrophic calcification, which is in accordance with the literature reviewed. Therefore, these phenomena must be taken into account in routine diagnosis services.
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http://dx.doi.org/10.1016/j.prp.2020.152975DOI Listing
June 2020

Hybrid ameloblastoma and central giant cell lesion: Challenge of early diagnosis.

J Clin Exp Dent 2020 Feb 1;12(2):e204-e208. Epub 2020 Feb 1.

Department of Pathology, Federal University of Santa Catarina, Florianopolis, Santa Catarina, Brazil.

Hybrid lesions encompass the occurrence of different entities in one lesion. A 67-year-old woman was referred to the Oral and Maxillofacial Surgery Service for treatment of mandibular Central Giant Cell Lesion (CGCL) previously diagnosed. Intraoral examination revealed edentulism and a painless swelling extending from the alveolar ridge to the buccal vestibule with hard consistency covered by normal mucosae, with unknown duration. Panoramic radiograph revealed a large, multilocular and well-defined radiolucent lesion extending from the region of left mandibular lateral incisor teeth to right mandibular first molar with no evidence of osseous perforation. Initially, a treatment with intralesional injection of corticosteroids was performed. After 18 months of treatment, an increase in size of the osteolytic lesion was noted. An incisional biopsy was carried out and the microscopic examination revealed a unicystic ameloblastoma associated to CGCL. It was performed marsupialization and later the enucleation of residual lesion. The follow-up remains being performed. Hybrid lesion, central giant cell lesion, ameloblastoma.
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http://dx.doi.org/10.4317/jced.56441DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7018476PMC
February 2020

Human Dental Pulp Stem Cells in Rat Mandibular Bone Defects.

Cells Tissues Organs 2019 15;207(3-4):138-148. Epub 2019 Nov 15.

Departamento de Ciências Morfológicas, Universidade Federal de Santa Catarina, Florianópolis, Brazil,

This study aimed to evaluate the use of human dental pulp stem cells (hDPSCs) in non-critical-sized mandibular bone defects in rats. hDPSCs from permanent teeth were isolated and engrafted in mandibular bone defects in rats for 7, 14, and 28 days; bone defects without cells formed the control group. Samples were evaluated by scanning electron microscopy (SEM), light microscopy (hematoxylin and eosin staining), and the regeneration area was measured by the Image J program. Before surgery procedures, the human dental pulp cells were characterized as dental pulp stem cells: fusiform morphology, plastic-adherent; expression of CD105, CD73, and CD90; lack of expression of CD45 and CD34, and differentiated into osteoblasts, adipocytes, and chondroblasts. The results indicated that within 7 days the control group presented a pronounced bone formation when compared with the treated group (p < 0.05). After 14 days, the treated group showed an increase in bone formation, but with no statistical difference among the groups (p > 0.05). In the final evaluated period there was no difference between the control group and the treated group (p > 0.05). There was a significant difference between 7 and 14 days (p < 0.05) and between 7 and 28 days (p < 0.05) in the treated group. In conclusion, there is no evidence that the use of hDPSCs in the conditions of this study could improve bone formation in non-critical-sized mandibular bone defects.
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http://dx.doi.org/10.1159/000502513DOI Listing
July 2020

Effects of sclerosing agents on head and neck hemangiomas: A systematic review.

J Clin Exp Dent 2019 Nov 1;11(11):e1033-e1044. Epub 2019 Nov 1.

Department of Pathology, Federal University of Santa Catarina (UFSC), Florianopolis, Brazil.

Background: The aim of this study was to systematically review the literature for studies that investigated the effects of sclerosing agents on head and neck hemangiomas.

Material And Methods: Clinical trials, cohort studies, and descriptive studies were considered eligible and selected in a two-phase process. Six main electronic databases, in addition to three grey literature databases, were searched. Risk of bias (RoB) was assessed using the "Meta-Analysis of Statistics Assessment and Review Instrument" checklist. From fifty-six considered eligible, five were finally included.

Results: One article were judged at low, one at moderate, and three at high RoB. The sclerosing agents investigated were sodium tetradecyl sulphate (n=2), ethanolamine oleate (n=1), pingyangmycin (n=1) and bleomycin (n=1). Overall, good results were achieved on the treatment of head and neck hemangiomas with intralesional sclerotherapy. Most commonly reported adverse effects included pain, swelling, fever, necrosis, transient facial palsy, and anorexia.

Conclusions: Considering the limited number of included studies, intralesional sclerotherapy on the management of HN hemangiomas presented overall good results with minor adverse reactions, especially in regards to smaller lesions. Sclerotherapy, sclerosing solutions, vascular neoplasms, hemangioma.
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http://dx.doi.org/10.4317/jced.56143DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825735PMC
November 2019

Primary intraosseous carcinoma of the maxilla arising from an odontogenic keratocyst: a case report and review of the literature.

Gen Dent 2019 Nov-Dec;67(6):26-32

A 47-year-old man was referred for treatment of a painful lesion of 5 months' duration located on the left side of the maxilla. A small perforation in the buccal cortex was observed during the intraoral examination. Cone beam computed tomography (CBCT) showed an extensive, well-delimited radiolucent lesion extending from the alveolar ridge to the nasal cavity. An incisional biopsy was performed, and a cystic lesion consistent with an odontogenic keratocyst (OKC) was observed microscopically. The initial treatment option was decompression to be followed by enucleation. However, 3 months after decompression of the lesion, the patient returned because there was a significant increase in the size of the perforation. A destructive lytic lesion that involved the left side of the maxilla and crossed the midline was evident in the CBCT. The examination of a second incisional biopsy specimen showed epithelial neoplasia comprising islands and projections toward the surface. There was abundant keratin deposition, resulting in the formation of pearls and plugs. A diagnosis of primary intraosseous carcinoma arising from an OKC was confirmed, and the patient underwent a maxillectomy. After 1 year of follow-up, there were no signs of recurrence.
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December 2019

Actinic cheilitis and lip squamous cell carcinoma: Literature review and new data from Brazil.

J Clin Exp Dent 2019 Jan 1;11(1):e62-e69. Epub 2019 Jan 1.

Department of Pathology, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.

Background: To investigate the prevalence of malignant and potentially malignant lesions of the lip in an oral pathology service and to compare these data with a literature review.

Material And Methods: A total of 3173 biopsy reports and histopathological records were analyzed. Cases with a histological diagnosis of actinic cheilitis (AC) with or without epithelial dysplasia, in situ carcinoma, or lip squamous cell carcinoma (LSCC) were included. A comprehensive literature review was conducted to investigate the prevalence of AC and/or LSCC.

Results: 124 cases (3.91%) were included, 75 (60.5%) had some degree of epithelial dysplasia and 31 (25.0%) were LSCC. Clinically, most of the lesions were diagnosed as AC (50.8%); however, eight cases clinically reported as AC were histologically diagnosed as LSCC. Regarding clinical characteristics, most individuals were fair-skinned male, with mean age of 54.3±12.3 years, and with a history of long-term solar exposure. Furthermore, 18 articles were selected from the literature, showing that the lower lip was predominantly affected and that most individuals were males, fair-skinned, and older than 40 years.

Conclusions: Since most of the cases diagnosed clinically as AC presented some degree of epithelial dysplasia, it is important to emphasize the value of biopsy and the histological evaluation of this lesion. Actinic cheilitis, Precancerous conditions, In situ carcinoma, Oral diagnosis, Mouth neoplasms.
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http://dx.doi.org/10.4317/jced.55133DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343992PMC
January 2019

Immunoexpression of SOX-2 in oral leukoplakia.

Oral Dis 2018 Nov 18;24(8):1449-1457. Epub 2018 Jul 18.

Pontifícia Universidade Católica do Paraná, Curitiba, Brasil.

Objective: This study was conducted to correlate and compare the immunoexpression of sex-determining region Y-box 2 (SOX-2) in oral leukoplakia (OL) lesions with that in normal buccal mucosa (control).

Materials And Methods: In this observational study, OL with low-risk (n = 34) and high-risk (n = 33) dysplasia and control samples (n = 25) were subjected to immunohistochemical analysis for SOX-2. In the epithelium, SOX-2 positive and negative cells, as well as semiautomatic segmentation of the immunopositive nuclear area were counted. Statistical tests included chi-square, one-way analysis of variance, Tukey, and Games-Howell. The level of significance was 5%.

Results: Groups with OL lesions (low and high-risk) showed higher mean numbers of SOX-2 positive cells (63.47 ± 25.70 and 68.18 ± 21.17) compared to the control group (45.85 ± 27.38) (p = 0.00). Groups with OL lesions (low and high-risk) exhibited higher mean positive nuclear area (0.24 ± 0.47 and 1.09 ± 2.06) compared to the control group (0.00 ± 0.01) (p = 0.01).

Conclusion: Oral leukoplakia lesions showed a higher expression of SOX-2, suggesting its contribution to the pathogenesis of OL.
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http://dx.doi.org/10.1111/odi.12922DOI Listing
November 2018

Expression of stem cell markers Nanog and Nestin in lip squamous cell carcinoma and actinic cheilitis.

Oral Dis 2018 Oct 28;24(7):1209-1216. Epub 2018 Jun 28.

Dentistry Graduate Program, Federal University of Santa Catarina, Florianopolis, Brazil.

Cancer stem cell (CSC) proteins have been observed in several lesions and are associated with tumor beginning, evolution, and resistance to treatment.

Objectives: To investigate the presence of NANOG, NESTIN, and β-tubulin in lip squamous cell carcinoma (LSCC), actinic cheilitis (AC), and normal epithelium (NE).

Materials And Methods: Thirty cases of LSCC, thirty cases of AC (both analyzed according to the WHO classification and AC according to the binary classification), and twenty cases of NE were submitted to an immunohistochemical study.

Results: NANOG was more expressed in the nuclei of AC compared to NE (p = 0.007), as well as in high-risk AC cases (p = 0.017) and well-differentiated LSCCs (no significance). There was an accumulation of nuclear NANOG from mild to moderate and severe ACs. NESTIN was significantly less present in NE compared to AC (p = 0.001) and LSCC (p = 0.003). There was a higher expression in severe dysplasia or high-risk AC and well-differentiated LSCC. These results indicate an upregulation of NANOG and NESTIN in the early stages of carcinogenesis. β-tubulin was intensely present in all lesions.

Conclusion: The results suggest an upregulation of NANOG and NESTIN in the biological behavior these diseases, mainly in the transformation from AC to LSCC.
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http://dx.doi.org/10.1111/odi.12891DOI Listing
October 2018

Histone modifications: A review about the presence of this epigenetic phenomenon in carcinogenesis.

Pathol Res Pract 2017 Nov 28;213(11):1329-1339. Epub 2017 Jun 28.

Department of Pathology, Federal University of Santa Catarina, Florianopolis, Santa Catarina, Brazil. Electronic address:

Among the epigenetic changes, histone acetylation has been recognized as a fundamental process that strongly affects gene expression regulation. Disrupt of this phenomenon has been linked to carcinogenesis. In this review, we analysed studies reporting the process of histone modification, the enzymes associated and affected genes concerning human malignancies and histone enzyme inhibitor drugs used in cancer treatment. Variable degrees of expression of HDACs (histone deacetylases) and HATs (histone acetyltransferases) are found in many human malignant tissues and the histones acetylation seems to influence different processes including the progression of cell cycle, the dynamics of chromosomes, DNA recombination, DNA repair and apoptosis. Thus, the control of aberrant activity and/or expression of these proteins have been favorable in treatment of diseases as cancer. HDACi have shown efficacy in clinical trials in solid and hematological malignancies. Therefore, the development and use of HDACs inhibitors are increasing, leading to continue studying these enzyme expressions and behavior, aiming to determine tumors that will respond better to this type of treatment.
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http://dx.doi.org/10.1016/j.prp.2017.06.013DOI Listing
November 2017

Effect of organic silicon, methylsulfonylmethane, and glucosamine sulfate in mandibular bone defects in rats.

Microsc Res Tech 2017 Nov 28;80(11):1161-1166. Epub 2017 Jul 28.

Morphology Sciences Department and Dentistry Graduate Program, Federal University of Santa Catarina, CEP: 88040-970, Florianópolis, SC, Brazil.

Organic silicon (OS), glucosamine sulfate (GS), and methylsulfonylmethane (MSM) have been related to bone and connective tissue health and have been considered as basic therapy for osteoarthrosis disorders. Therefore, the aim was to analyze the effect of the association of these three components in mandibular bone defects in rats. Nine rats were used for histocompatibility test. In each animal was implanted the composition (70% OS, 15% GS, 15% MSM) and gutta percha (control) under the dorsal subcutaneous tissue. The samples were collected at 7, 14, and 21 days post-surgery and inflammatory events analyzed. In sequence, the composition was engrafted in mandibular bone defects of nine rats; bone defects without treatment were the control group. Analyses were performed at 7, 14, and 28 days post-surgery and samples were evaluated by scanning electron microscopy (SEM). For the histocompatibility test, both groups had a moderate inflammatory process at 7 days post-surgery and mild inflammatory process at 14 and 21 days. But in SEM analysis, the composition promotes an extensive reabsorption in cortical and crest alveolar bone, and great tooth root reabsorption. In conclusion, although the composition had positive result in the histocompatibility test, its direct application in mandibular bone defects caused intense resorption.
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http://dx.doi.org/10.1002/jemt.22911DOI Listing
November 2017

Immunohistochemical expression of DNA methyltransferases 1, 3a, and 3b in actinic cheilitis and lip squamous cell carcinomas.

J Oral Pathol Med 2016 Nov 9;45(10):774-779. Epub 2016 May 9.

Pathology Department and Dentistry Graduate Program, Federal University of Santa Catarina, Florianopolis, Santa Catarina, Brazil.

Background: Epigenetic modifications, including DNA methylation of tumor suppressor genes carried out by DNA methyltransferases (DNMTs), are important events in carcinogenesis. Although there are studies concerning to its expression in several cancer types, DNMTs expression pattern is not known in photoinduced lip carcinogenesis. The aim of this study was to investigate the immunoexpression of DNMTs 1, 3a, and 3b in lip precancerous lesion (actinic cheilitis) and cancer.

Methods: Thirty cases of actinic cheilitis (AC), thirty cases of lip squamous cell carcinoma (LSCC), and twenty cases of non-neoplastic tissue (NNT) were selected for immunohistochemical investigation of DNMTs 1, 3a, and 3b.

Results: Nuclear DNMT 1 immunoreactivity was significantly higher in the LSCC group (68.6%) compared with NNT (47%), and nuclear DNMT 3b was higher in LSCC (70.9%) than in NNT (37.9%) and in AC (44%). Only DNMT 3a showed both higher nuclear and cytoplasmic expression in AC (35.9% and 35.5%, respectively) than in NNT (4.4% and 16.1%, respectively) and LSCC (8.8% and 13.2%, respectively) (P < 0.05).

Conclusions: The results suggested that DNMT 3a could play a key role in the methylation process of initial steps of UV carcinogenesis present in AC while DNMT 3b could be responsible for de novo methylation in already established lip cancer.
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http://dx.doi.org/10.1111/jop.12453DOI Listing
November 2016

Immunohistochemical expression of matrix metalloproteinase-1, matrix metalloproteinase-2 and matrix metalloproteinase-9, myofibroblasts and Ki-67 in actinic cheilitis and lip squamous cell carcinoma.

Int J Exp Pathol 2015 Oct 30;96(5):311-8. Epub 2015 Oct 30.

Pathology Department and Dentistry Graduate Program, Federal University of Santa Catarina, Florianopolis, Brazil.

Matrix metalloproteinases (MMPs), myofibroblasts (MFs) and epithelial proliferation have key roles in neoplastic progression. In this study immunoexpression of MMP-1, MMP-2 and MMP-9, presence of MFs and the epithelial proliferation index were investigated in actinic cheilitis (AC), lip squamous cell carcinoma (LSCC) and mucocele (MUC). Thirty cases of AC, thirty cases of LSCC and twenty cases of MUC were selected for immunohistochemical investigation of the proteins MMP-1, MMP-2, MMP-9, α-smooth muscle actin (α-SMA) and Ki-67. The MMP-1 expression in the epithelial component was higher in the AC than the MUC and LSCC. In the connective tissue, the expression was higher in the LSCC. MMP-2 showed lower epithelial and stromal immunostaining in the LSCC when compared to the AC and MUC. The epithelial staining for MMP-9 was higher in the AC when compared to the LSCC. However, in the connective tissue, the expression was lower in the AC compared to other lesions. The cell proliferation rate was increased in proportion to the severity of dysplasia in the AC, while in the LSCC it was higher in well-differentiated lesions compared to moderately differentiated. There were no statistically significant differences in number of MFs present in the lesions studied. The results suggest that MMPs could affect the biological behaviour of ACs and LSCCs inasmuch as they could participate in the development and progression from premalignant lesions to malignant lesions.
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http://dx.doi.org/10.1111/iep.12140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4693555PMC
October 2015

Evaluation of AgNORs in Oral Potentially Malignant Lesions.

J Oncol 2015 31;2015:218280. Epub 2015 Aug 31.

Department of Pathology, Health Sciences Center, Federal University of Santa Catarina, University Campus, Trindade, 88040-370 Florianópolis, SC, Brazil.

Oral squamous cell carcinoma (OSCC) is usually preceded by detectable mucosal changes, as leukoplakias and erythroplakia. Histologically, these lesions can range from hyperkeratosis and acanthosis to epithelial dysplasia and even OSCC. The aim of this study was to investigate the proliferative activity, using AgNORs quantification proteins, in low- and high-risk oral epithelial dysplasia, OSCC, and nondysplastic epithelium (inflammatory fibrous hyperplasia). The sample was divided into 4 groups: G1: 10 cases of inflammatory fibrous hyperplasia (IFH), G2: 11 cases of low-risk epithelial dysplasia (LD), G3: 10 cases of high-risk epithelial dysplasia (HD), and G4: 11 cases of OSCC. The quantitative analysis was performed using an image processing software in photomicrographs at 1000x magnification. The one-way ANOVA was used for comparison of the mean AgNORs counts between the study groups. The mean AgNORs count was significantly higher (P ≤ 0.01) in OSCC when compared to IFH and the LD; however, it was not statistically different from HD. The mean number of LD was significantly lower than the HD and OSCC, with no difference related to IFH. AgNORs quantification can be an important and cheap method to help in the determination of the degree of epithelial dysplasia and, consequently, in the analysis of their potential for malignant transformation.
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http://dx.doi.org/10.1155/2015/218280DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568384PMC
September 2015

A Rare Case of Mucoepidermoid Carcinoma ex Pleomorphic Adenoma arising in Minor Salivary Gland: Histopathological and Immunohistochemical Analysis.

J Contemp Dent Pract 2015 07 1;16(7):603-6. Epub 2015 Jul 1.

Department of Pathology, Health Sciences Center, Federal University of Santa Catarina, Santa Catarina, Brazil.

Mucoepidermoid carcinoma ex pleomorphic adenoma (MCxPA) is a rare salivary gland tumor predominantly found in major salivary glands. A case of MCxPA involving the soft tissue and bone of the retromolar region of a 26-year-old man is presented. The histopathological features revealed a neoplasm with predominance of pleomorphic adenoma (PA) elements, and presence of mucoepidermoid carcinoma malignant epithelial cells in several areas. Histochemical and immunohistochemical studies were positive for periodic acid Schiff, alcian blue, cytokeratins 7, 13, 14, and 19, Bcl-2, c-erbB-2, FGF-2 and maspin in the malignant areas. The patient underwent a partial resection of the left side of the mandible with neck dissection and MCxPA diagnosis was confirmed.
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http://dx.doi.org/10.5005/jp-journals-10024-1728DOI Listing
July 2015

Temporo-spatial analysis of Osterix, HNK1 and Sox10 during odontogenesis and maxillaries osteogenesis.

Tissue Cell 2015 Oct 29;47(5):465-70. Epub 2015 Jul 29.

Morphology Sciences Department, Federal University of Santa Catarina, Florianopolis, SC, Brazil. Electronic address:

Cell differentiation is essential for maxillaries and tooth development. Facial mesenchymal tissue is formed by neural crest cells (NC). These cells are highly migratory, giving rise to various cell types, considered with a high level of plasticity, indicating that they contain progenitor cells with a great power of differentiation. In this study, it was analyzed the presence of NC cell progenitors and mesenchymal stem cells (MSC) during maxillaries osteogenesis and odontogenesis in rats. Histological slides were collected in two phases: embryonic age of 15 and 17 days; 2, 4 and 7 days after birth. Immunohistochemistry for MSC markers (Osterix) and NC cells (Sox10, HNK1) was performed. The results showed positive expression for Osterix and HNK1 in undifferentiated ectomesenchymal cells in early and late stages; Sox10 was present only in early stages in undifferentiated cells. All markers were present in differentiated cells. Although the experiments performed do not allow us to explain a possible role for Osx, HNK1 and Sox10 in both differentiated and undifferentiated cells during osteogenesis and odontogenesis, it had shown important results not yet described: the presence of HNK1 and Sox10 in osteoblasts and odontoblasts in late development stages and in the tooth germ epithelial cells and ameloblasts.
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http://dx.doi.org/10.1016/j.tice.2015.07.007DOI Listing
October 2015

Immunolocalization of bone morphogenetic protein 2 during the early healing events after guided bone regeneration.

Oral Surg Oral Med Oral Pathol Oral Radiol 2012 Apr;113(4):533-41

Department of Stomatology, University of São Paulo, São Paulo, Brazil.

Objective: The objective of this study was to evaluate the immunolocalization of bone morphogenetic protein 2 (BMP-2) after autogenous block grafting covered or not with an e-PTFE membrane.

Study Design: Forty-eight rats were divided into 2 groups, autogenous block graft (B) and autogenous block graft + e-PTFE membrane (MB), and were evaluated by immunohistochemistry at baseline and 3, 7, 14, 21, and 45 days.

Results: The largest number of positive cells in the recipient bed was observed after 3 days in both groups. At the graft border, the largest number of positive cells was seen after 7 days in group B and after 14 days in group MB. The highest proportion of staining in the graft was observed after 3 days in group B and after 21 days in group MB.

Conclusions: High proportions of stain were related to intense revascularization and osteogenesis. Except for the interface, BMP-2 staining occurred later in group MB than in group B in all structures analyzed.
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http://dx.doi.org/10.1016/j.oooo.2011.09.016DOI Listing
April 2012

Immunohistochemical expression of Rho GTPases in ameloblastomas.

J Oral Pathol Med 2012 May 18;41(5):400-7. Epub 2011 Nov 18.

Pathology Department, Federal University of Santa Catarina, Florianopolis, SC, Brazil.

Rho GTPases are proteins that regulate cell cycle, shape, polarization, invasion, migration, and apoptosis, which are important characteristics of normal and neoplastic cells. Rho GTPases expression has been reported in normal tooth germ and several pathologies; however, it has not been evaluated in ameloblastomas. The aim of this study was to analyze the expression and distribution of RhoA, RhoB, Rac1, and Cdc42 Rho GTPases in solid and unicystic ameloblastomas. Three-micrometer sections from paraffin-embedded specimens were evaluated by using an avidin-biotin immunohistochemical method with antibodies against the proteins mentioned above. RhoA and RhoB staining was observed in a high number of cells (P < 0.05) and greater intensity in non-polarized ones. Rac1 was not observed, and Cdc42 did not show any statistical differences between the number of non-polarized and basal positive cells (P > 0.05). Upon comparing the studied ameloblastomas, a higher number of positive cells in the unicystic variant was observed than that in the solid one (P < 0,05). The results obtained suggest that these GTPases could play a role in the ameloblastoma neoplastic epithelial cell phenotype determination (polarized or non-polarized), as well as in variant (solid or unicystic) and subtype (follicular or plexiform) determination. Furthermore, they could participate in solid ameloblastoma invasion mechanisms.
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http://dx.doi.org/10.1111/j.1600-0714.2011.01108.xDOI Listing
May 2012

Techniques for precancerous lesion diagnosis.

J Oncol 2011 13;2011:326094. Epub 2011 Jan 13.

Postgraduate Program of the Federal University of Santa Catarina, 88040-370 Florianopolis, SC, Brazil.

The development of the oral squamous cell carcinoma (OSCC) is a multistep process that requires the accumulation of multiple genetic alterations usually preceded by detectable mucosal changes, most often leukoplakias and erythroplakias. The clinical appearance of oral precancerous lesions and their degree of epithelium dysplasia suggests the malignization potential. Several techniques have been developed to improve the clinical and cytological diagnosis of oral precancerous lesions. The present paper reviews the main techniques used to improve premalignant lesion diagnosis.
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http://dx.doi.org/10.1155/2011/326094DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3026985PMC
July 2011

Immunohistochemical expression of DNA methyltransferases 1, 3a and 3b in oral leukoplakias and squamous cell carcinomas.

Arch Oral Biol 2010 Dec 16;55(12):1024-30. Epub 2010 Oct 16.

School of Dentistry, Oral Medicine Division, Pontifical Catholic University of Rio Grande do Sul, Brazil.

Unlabelled: Over-expression of DNA methyltransferases DNMT1, DNMT3a and DNMT3b has been reported in various cancers and precancerous lesions.

Objective: To investigate DNMT1, DNMT3a and DNMT3b enzymes in oral squamous cell carcinoma (SCC) and leukoplakia, and their relationship with histopathologic/clinical parameters.

Study Design: Immunohistochemistry was carried out to evaluate the three DNMTs in 60 samples of oral SCC and 37 samples of oral leukoplakia.

Results: DNMT3a immunoreactivity in the three groups of oral SCC (39.8%) was significantly higher than in control (22.6%) (ANOVA, Student-Newman-Keuls test, P<0.05), but not when compared to oral leukoplakia groups (28.2%). For DNMT1 and DNMT3b, there were no statistically significant differences between oral SCC groups (65% and 74.7%), oral leukoplakia groups (68.3% and 70.9%) and control (65.4% and 76.5%). There was a significantly higher mean percentage of DNMT1 immunoreactivity in non-smokers (ANOVA, P=0.048), and a higher DNMT3a immunoreactivity in alcohol users (ANOVA, P=0.01).

Conclusions: Higher DNMT3a immunopositivity may be associated with oral SCC and alcohol use, whilst lower levels of DNMT1 may be related with smoking habit. However, there was a significantly higher mean percentage of DNMT1 immunoreactivity in non-smokers (ANOVA, P=0.048), and a higher DNMT3a immunoreactivity in alcohol users (ANOVA, P=0.010).
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http://dx.doi.org/10.1016/j.archoralbio.2010.08.009DOI Listing
December 2010

Expression of extracellular matrix proteins in adenomatoid odontogenic tumor.

J Oral Pathol Med 2010 Mar 11;39(3):230-5. Epub 2010 Jan 11.

Pathology Department, Health Sciences Center, Federal University of Santa Catarina, Florianopolis.

Altered expression of extracellular matrix (ECM) components has been reported in several pathologies; however, few ECM proteins have been evaluated in adenomatoid odontogenic tumor (AOT). The aim of this study was to analyze the expression and distribution of the ECM proteoglycans: biglycan and decorin; and glycoproteins: osteonectin, osteopontin, bone sialoprotein and osteocalcin in the AOT. Three-micrometer sections from paraffin-embedded specimens were evaluated employing a streptavidin-biotin immunohistochemical method with the antibodies against the proteins previously cited. Only the osteonectin was expressed in the epithelial cells. The eosinophilic amorphous material and the connective tissue showed expression of all components studied. The calcification foci expressed only osteopontin. In conclusion, the low expression of the components studied in neoplastic epithelial cells suggests that the epithelial cells act probably as stimulators of the expression by the stroma, which in turn can act as agonist or antagonist of the tumor growth. These results suggest that the components studied probably have a key role in the biological behavior of the AOT.
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http://dx.doi.org/10.1111/j.1600-0714.2009.00846.xDOI Listing
March 2010

Atypical presentation of oral basaloid squamous cell carcinoma.

J Contemp Dent Pract 2009 Mar 1;10(2):98-104. Epub 2009 Mar 1.

Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil.

Aim: The purpose of this report is to present the clinical and histological features of a basaloid squamous cell carcinoma (BSCC) occurring in the retromolar trigone of a 59-year-old man and to relate its immunohistochemical characteristics.

Background: BSCC is an aggressive distinct variant of squamous cell carcinoma (SCC) requiring recognition as a separate entity from SCC due to its peculiar behavior.

Case Report: A clinical examination revealed a 12x07x07 mm nodular mass with a rubbery consistency, defined borders, covered by reddish mucosa and an absence of bleeding upon palpation. Histologically, nests and cords of closely packed, moderately pleomorphic basaloid cells with nuclear palisading along the periphery of the neoplastic nests surrounded by a fibrous stroma were found.

Summary: Since this tumor can mimic other neoplasms such as adenoid cystic carcinoma, neuroendocrine carcinoma, and basal cell adenocarcinoma, histological features are essential to differentiate between them. Furthermore, immunohistochemical testing can provide valuable diagnostic information that can have a profound impact on treatment options and the prognosis.

Clinical Significance: BSCC needs to be differentiated from other neoplasms as early as possible because of its adverse prognosis. Clinicians are advised to conduct a mucosal evaluation during oral examinations and take a thorough medical history which could ultimately save the life of a patient.
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March 2009

Painful deviation of the mandible.

Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009 Jun 20;107(6):749-53. Epub 2009 Feb 20.

Department of Oral and Maxillofacial Surgery, Federal University of Santa Catarina, Florianópolis, Brazil.

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http://dx.doi.org/10.1016/j.tripleo.2009.01.002DOI Listing
June 2009

Recurring acinic cell carcinoma of the buccal mucosa: a case report.

Quintessence Int 2007 Apr;38(4):289-94

Division of Oral Medicine, Department of Stomatology, University of São Paulo, Brazil.

A case of acinic cell adenocarcinoma of the left facial area of 10-years' duration in a 29-year-old man is presented. The patient reported surgical resection of a nodular lesion in the left buccal mucosa 8 years earlier in another hospital. Since then, the lesion recurred 3 times within 2 years. The first lesion and 2 recurrent ones were surgically removed. With the third recurrent lesion, the patient did not return promptly for treatment and was directed to our clinic after 6 years. The clinical, tomographic, immunohistochemical, and therapeutic aspects are analyzed.
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April 2007

Idiopathic hyperplasia of the sublingual glands in totally or partially edentulous individuals.

Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2007 Mar 25;103(3):374-7. Epub 2006 Sep 25.

Department of Oral Diagnosis, School of Dentistry, University of São Paulo, São Paulo, SP, Brazil.

Objective: To determine the possible etiologic factors associated with the development of idiopathic hyperplasia of the sublingual gland (IHSG).

Study Design: Fifty partially or totally edentulous patients were divided into 2 groups, each with 25 subjects, matched by sex. Group 1 was made up of patients with IHSG, whereas group 2 consisted of patients without IHSG. Patients of both groups were investigated with respect to the use of prostheses, systemic medication, systemic diseases, smoking habits, alcohol consumption, and xerostomia. In addition, patients of group 1 were examined by occlusal radiography, and in 6 of them, a biopsy of the sublingual gland swelling was taken for histopathologic investigation. The Fisher exact test was used, with significance set for P < or = .05.

Results: The comparative analysis revealed no statistical difference between the 2 groups concerning all factors investigated (P > .05). Radiographic examination did not detect any presence of salivary calculi; the histopathologic findings revealed either chronic sialadenitis (4 cases) or normal glandular tissue (2 cases).

Conclusion: Our clinical and histopathologic analyses confirm that IHSG is a harmless condition for which no surgical treatment is required. The etiologic investigation did not find any local or systemic factors significantly associated with the development of IHSG. The absence of posterior teeth remains as the only known predisposing factor to be strongly linked to this condition.
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http://dx.doi.org/10.1016/j.tripleo.2006.04.012DOI Listing
March 2007

Congenital teratoid cyst of the floor of the mouth.

Otolaryngol Head Neck Surg 2007 Jan;136(1):134-6

Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil.

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http://dx.doi.org/10.1016/j.otohns.2006.03.021DOI Listing
January 2007