Publications by authors named "Fariba Khoshzaban"

11 Publications

  • Page 1 of 1

Effect of a Natural Eye Drop, Made of Mucilage on Improvement of Dry Eye Symptoms: A Randomized, Double-blind Clinical Trial.

Iran J Pharm Res 2019 ;18(3):1602-1611

Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Dry eye disease is a relatively common eye disorder associated with decrease in quality of life. In this study, efficacy of an eye drop of mucilage on symptoms of dry eye disease was evaluated. In a randomized, double-blind, placebo-controlled clinical trial, sixty dry eye patients with ocular symptoms and total Ocular Surface Disease Index (OSDI) score of ≥12 were randomly assigned to receive either a natural ophthalmic drop, made of mucilage or placebo 4 times a day for 6 weeks. The patients were evaluated at pretreatment (baseline), weeks 4 and 6 post-treatment. The evaluation of the efficacy and safety were conducted based on the OSDI questionnaire, the noninvasive tear film break-up time (NI-BUT) with keratograph, the Schirmer test without anesthesia, and the osmolarity test, as well as by monitoring possible adverse events. After 6 weeks, within group analysis showed a significant improvement in total OSDI score ( < 0.001). In addition, between group comparison revealed a significant improvement in the OSDI score of the intervention group ( < 0.001). Although, NI-BUT was significantly improved in the group ( = 0.004), however no statistically significant difference was observed in between group analysis. There were no significant differences between two groups, or significant changes within the groups in the Schirmer test without anesthesia and the osmolarity test. No serious adverse events were reported. In conclusion, mucilage is a natural, inexpensiveness, and safe lubricant polymer that could have beneficial ocular effects on subjective symptoms of the patients with dry eye disease.
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http://dx.doi.org/10.22037/ijpr.2019.1100717DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6934964PMC
January 2019

Evaluation of the effects of Rumex obtusifolius seed and leaf extracts against Acanthamoeba: An in vitro study.

Infect Disord Drug Targets 2020 Apr 22. Epub 2020 Apr 22.

Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran. Iran.

Background: Acanthamoebiasis treatment is a major and challenging problem due to the presence of resistant cyst form. Many herbal extracts and their derivatives have been used against trophozoites and cysts of Acanthamoeba, but no effective therapeutic agent has yet been discovered. Therefore, the present study aimed to evaluate the effect of Rumex obtusifolius (R. obtusifolius) extracts against a clinical strain of Acanthamoeba genotype T4 in vitro.

Methods: In this experimental study, after genotyping the clinical isolate, the hydroalcohlic extracts of R. obtusifolius seeds and leaves were prepared. Different concentrations (1.25, 2.5, 5 and 10 mg/ml) of extracts were tested in triplicate (24, 48 and 72h) on trophozoites and cysts of Acanthamoeba. Mortality of the parasite was assessed by trypan blue vital staining and flow cytometry analysis.

Results: Results showed that the extract of R. obtusifolius leaves at the concentration of 10 mg/ml killed 100% of trophozoites and cysts after 72 h. However, the seed extract of R. obtusifolius had weak inhibitory effects on trophozoites and cysts of Acanthamoeba. In the presence of 10 mg/ml of hydroalcohlic seed extract of R.obtusifolius in culture medium after 72 h, 28.6% of trophozoites and 0% of cysts of Acanthamoeba were killed. After analysis by flow cytometry, seeds and leaves extracts indicated apoptosis effect. Seed and leaf of extracts caused 2.6% and 0.4% percent apoptosis.

Conclusion: These extracts are not promising candidates for further medicine development on acanthamoebiasis. Nonetheless, further researches are necessary to clarify effective fractions of seed and leaf extracts of R. obtusifolius and their mechanisms of action.
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http://dx.doi.org/10.2174/1871526520666200422111044DOI Listing
April 2020

Contamination of swimming pools and hot tubs biofilms with Acanthamoeba.

Acta Parasitol 2018 Mar;63(1):147-153

Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

The members of Acanthamoeba genus are ubiquitous amoeba which could be a pathogenic parasite. The amoeba is resistant to the common chlorine concentration that used for disinfecting the swimming pool water. Therefore, the pools can be suitable environments for the survival and multiplication of the amoeba. In this cross sectional study, 10 indoor recreational water centers from different regions of Tabriz city were selected and sampling was done from fixed and floating biofilms of the swimming pools and hot tubs. The samples were cultured and monitored for the presence of amoeba cyst or trophozoite. For molecular identification of Acanthamoeba, PCR (polymerase chain reaction) and sequencing were conducted based on genus specific fragment of 18S ribosomal DNA (Rns). Acanthamoeba contamination was observed in 6 centers of 10 recreational centers. Based on the amoeba isolation from fixed and floating biofilms, 2 (20%) swimming pools, and 5 (50%) hot tubs were contaminated. Based on the type of the sample, the highest contamination was found in the hot tub water (40%) and the least was found in the swimming pools water (10%) and fixed biofilms of the swimming pools (10%). Out of 8 isolates, 5 (62.5%) were shown expected product in PCR amplification. Sequence analysis showed that Acanthamoeba isolates belonged to the T3 and T4 genotypes. The study revealed a high degree of contamination in the indoor recreational water centers in Tabriz city. So, it is essential to pay closer attention to the hygiene of swimming pools and hot tubs.
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http://dx.doi.org/10.1515/ap-2018-0016DOI Listing
March 2018

Characterization of extracellular proteases of Acanthamoeba genotype T4 isolated from different sources in Iran.

Parasitol Res 2017 Dec 26;116(12):3373-3380. Epub 2017 Oct 26.

Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

The members of the genus Acanthamoeba are ubiquitous, free-living amoebae found in various environments. The amoebae can cause severe complications in both, immunocompetent and immunocompromised individuals. The aim of this study was to characterize extracellular proteases of Acanthamoeba isolates from different sources belonging to genotype T4 as well as the determination of the pathogenicity potential to correlate pathogenicity with protease activity and protease banding pattern. A total of 19 isolates (11 clinical and 8 environmental) were cultured axenically, then the pathogenicity of the isolates was assessed by osmo- and thermo- tolerance tests. An applied colorimetric method using azocasein as a substrate was used for the extracellular protease activity assay. Protease characterization was carried out by zymography analysis with and without protease inhibitors. Assessment of the pathogenicity potential using physical parameters revealed that 2 (25%), 2 (25%), and 4 (50%) of the environmental isolates were potential pathogens, weak potential pathogens, and non-pathogens, respectively. According to our results, this protease activity assay can be a powerful tool for differentiating pathogenic and non-pathogenic strains of Acanthamoeba.
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http://dx.doi.org/10.1007/s00436-017-5656-yDOI Listing
December 2017

Lifestyle Determinants on Prevention and Improvement of Dry Eye Disease from the Perspective of Iranian Traditional Medicine.

Iran J Med Sci 2016 May;41(3 Suppl):S39

Department of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Background: Dry eye disease is one of the most common public health problems in the field of ophthalmology that causes decreased physical, psychological, social and occupational functioning, and interferes with daily activities like reading, driving, computer work and watching TV. The effect of this disease on quality of life is similar to severe migraine and angina. In addition, the severity of the disease is closely related to depression. Outbreaks are increasing with the use of contact lenses and refractive surgery such as LASIK. The purpose of this paper is to assess the causes of dry eye and its prevention by the principles of hygiene.

Methods: In this review study, traditional medicine resources like Al canon fil tibb, Sharh-al-Al Asbab and Exir-e-azam have been studied with engine motors such as Google Scholar and PubMed.

Results: From the perspective of Iranian traditional medicine, the principles of maintaining health include air, food and drink, exercise and rest, sleep and wakefulness, mental state, retention of essential materials and depletion of wastes from the body. Proper performance of each principle is useful for the prevention of disease in different organs, including the eyes. Recommendations for the prevention or treatment of dry eye disease include the prevention of dryness in the eye or the entire body. In addition, the stomach and brain are important to eye health. Accordingly, one of the most effective managements of dry eye disease is avoiding foods that affect the eye in this way; for example garlic and onion.

Conclusion: Maintaining eye health is related to aspects of physical and mental health of the whole body. Therefore, codification of an integrated plan that contains eating and sleeping patterns, exercise, general clearing, eye clearing, and mental health is essential for treating dry eye disease.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5103546PMC
May 2016

Comparing the Healing Effects of Arnebia euchroma Ointment With Petrolatum on the Ulcers Caused by Fractional CO2 Laser: A Single-Blinded Clinical Trial.

Iran Red Crescent Med J 2014 Oct 5;16(10):e16239. Epub 2014 Oct 5.

Traditional Medicine Clinical Trial Research Center, Shahed University, Tehran, IR Iran.

Background: Arnebia euchroma ointment (AEO) has been used in Iranian traditional medicine for burn wound healing.

Objectives: The aim of this study is to evaluate wound healing efficacy of AEO in burn wounds after fractional Co2 laser.

Patients And Methods: This split-face, single-blinded, single-center clinical study was performed in Shohada-e-Tajrish Hospital, Tehran, Iran. A total of 26 subjects with facial acne scar, who were to receive fractional CO2 laser resurfacing were recruited. After laser procedure, AEO was applied to one side of the face and petrolatum on the other side for wound healing. Digital photographs were taken from acne scar area before resurfacing and on each of the assessment sessions. Three researchers, who were unaware of the applied medications, assessed these digital photographs for erythema, edema, epithelial confluence, crusting/scabbing, and general wound appearance. Subject's irritations such as dryness and itching were evaluated on the second, fifth, and seventh days.

Results: Our study indicated higher epithelial confluence and general wound appearance scores (P = 0.045 for both) and less erythema and edema on fifth day in petrolatum (P = 0.009 and P = 0.034, respectively). The results showed less crusting and erythema (P = 0.016 and P = 0.035, respectively) and higher general wound appearance scores in petrolatum on the second day (P = 0.035 and P = 0.001, respectively). Dryness was the most common subjective complaint in both groups; however, it was more severe in AEO, especially on the second day (P = 0.023).

Conclusions: Despite the healing effects of AEO in burn wounds, petrolatum was more effective than AEO in post-laser wound.
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http://dx.doi.org/10.5812/ircmj.16239DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4270663PMC
October 2014

Diet and food in Iranian traditional medicine: hints for further research.

Int J Prev Med 2014 Nov;5(11):1480-1

Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, I.R. Iran.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4274557PMC
November 2014

Peganum harmala Aqueous and Ethanol Extracts Effects on Lesions Caused by Leishmania major (MRHO/IR/75/ER) in BALB/c Mice.

Jundishapur J Microbiol 2014 Jul 1;7(7):e10992. Epub 2014 Jul 1.

Department of Parasitology and Mycology, Faculty of Medical Sciences, Shahed University, Tehran, IR Iran.

Background: Leishmaniasis is one of the six most common parasitic infections in the tropical regions. There are different therapeutic modalities, however therapeutic resistance is developed and resulted in numerous problems. Therefore, evaluation of other therapeutic modalities is performed extensively.

Objectives: The current study aimed to compare the therapeutic response of cutaneous leishmaniasis with Glucantime and Peganum harmala extracts (aqueous and ethanol) in the animal model.

Materials And Methods: The therapeutic response of Leishmania major to Glucantime and P. harmala extracts (aqueous and ethanol) in animal model was studied in BALB/c mice. These mice were divided into four groups according to receiving either one of these three agents, and the control group. The therapeutic response was evaluated according to the parasitic load before and after treatment and also with measuring the size of the lesions.

Results: The results showed that ethanol extract of P. harmala had good therapeutic efficacy in treatment of lesions in mice (P < 0.05), and the efficacy was significant in the eighth week after the treatment. There was also a statistically significant difference between the groups regarding the parasitic load (P < 0.05).

Conclusions: According to the current study results, it may be concluded that ethanol extract of P. harmala is efficient in the treatment of cutaneous leishmaniasis, and the efficiency is comparable with that of Glucantime.
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http://dx.doi.org/10.5812/jjm.10992DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4216572PMC
July 2014

Effect of Arnebia euchroma ointment on post-laser wound healing in rats.

J Cosmet Laser Ther 2015 Feb 27;17(1):41-5. Epub 2014 Oct 27.

Traditional Medicine Clinical Trial Research Center, Shahed University , Tehran , Iran.

Introduction: Arnebia euchroma ointment has been used in Iranian Traditional Medicine for burn wound healing. The aim of this study is to evaluate wound healing efficacy of A. euchroma ointment on wounds induced after fractional CO2 laser in rats.

Material And Methods: In this study, after anesthetizing two bilateral burn wounds were induced on dorsal skin of the rat using fractional ablative CO2 laser. After applying laser, A. euchroma ointment, petrolatum, and silver sulfadiazine cream were used topically on wounds twice daily for 10 days. Digital photographs were captured from the wound surfaces every day. At the end of the study, two blinded dermatologists observed the photograph of 3rd, 5th, 7th, and 9th days after laser injury and assessed erythema, crusting/scabbing, epithelial confluence, and general wound appearance to determine the efficacy of wound healing. These wound-healing parameters were assessed using the 5-point scales.

Results: This study showed significantly less erythema and crusting (P = 0.024 and P = 0.004, respectively) on 9th day and higher epithelial confluence and general wound appearance scores on 7th (P = 0.037 and p = 0.016, respectively) and 9th days (P = 0.008 and P = 0.016, respectively) in A. euchroma ointment compared with other groups.

Conclusion: This study showed A. euchroma ointment has good healing effects on post-laser wounds in rats.
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http://dx.doi.org/10.3109/14764172.2014.968583DOI Listing
February 2015

Enhancement of immune response induced by DNA vaccine cocktail expressing complete LACK and TSA genes against Leishmania major.

APMIS 2013 Apr 18;121(4):290-8. Epub 2012 Sep 18.

Department of Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Leishmaniasis is an important disease in humans. Leishmania homologue of receptor for Activated C Kinase (LACK) and thiol specific antioxidant (TSA) as immuno-dominant antigens of Leishmania major are considered the most promising molecules for a DNA vaccine. We constructed a DNA cocktail, containing plasmids encoding LACK and TSA genes of Leishmania major and evaluated the immune response and survival rate in BALB/c mice. IgG and Interferon gamma values were noticeably increased in the immunized group with DNA cocktail vaccine, which were significantly higher than those in the single-gene vaccinated and control groups (p < 0.05) following the immunization and after challenging with Leishmania major. Interleukin 4 values were decreased in all immunized groups, but only in DNA vaccine cocktail and single-gene vaccination with pc-LACK there were statistical differences with control groups (p > 0.05). The immunized mice with the cocktail DNA vaccine presented a considerable reduction in diameter of lesion compared to other groups and a significant difference was observed (p < 0.05) in this regard. The survival time of the immunized mice with the cocktail DNA vaccine was significantly higher than that in the other groups (p < 0.05) after their being challenged with Leishmania major. The findings of this study indicated that the cocktail DNA vaccine increased the cellular response and survival rate and induced protection against infection with Leishmania in the mice.
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http://dx.doi.org/10.1111/j.1600-0463.2012.02968.xDOI Listing
April 2013

Comparing the effect of IL-12 genetic adjuvant and alum non-genetic adjuvant on the efficiency of the cocktail DNA vaccine containing plasmids encoding SAG-1 and ROP-2 of Toxoplasma gondii.

Parasitol Res 2012 Jul 21;111(1):403-11. Epub 2012 Feb 21.

Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Various methods are available for enhancing the potency of DNA vaccines, including employment of different forms of adjuvant. The current study was carried out to evaluate and compare the effects of genetic and non-genetic adjuvants on the immune response stimulated by DNA vaccine. Thus, two adjuvants, IL-12 (genetic adjuvant) and aluminum hydroxide (alum, non-genetic adjuvant), were used with cocktail DNA vaccine containing plasmids encoding complete rhoptry antigen 2 (ROP-2) and surface major antigen 1 (SAG-1) of Toxoplasma gondii. The efficacy of pcROP2+pcSAG1 in stimulation of the immune response against toxoplasmosis with and without adjuvant was evaluated in female BALB/c mice by measuring the level of total IgG antibody and cytokines. The results obtained indicated that after challenging the mice with the fatal RH strain of T. gondii, the survival rates of mice immunized with pcROP2+pcSAG1 (DNA cocktail), pcSAG1+pcROP2+alum, and pcSAG1+pcROP2+IL-12 were significantly greater than that of the control groups (p<0.05). Moreover, measurement of total IgG antibody indicated the significant difference between the control and experimental groups (p<0.05). Finally, the results obtained by measurement of cytokines (IFN-γ and IL-4) showed high levels of IFN-γ and low levels of IL-4 in groups vaccinated with pcROP2+pcSAG1 (DNA cocktail), pcSAG1+pcROP2+alum, and pcSAG1+pcROP2+IL-12 as the experiment groups, in comparison with the controls groups (PBS, pc-DNA3, alum+PBS, and pCAGGS-IL-12+pcDNA3). The results of the study showed that use of adjuvants (IL-12 and alum) coincident with DNA cocktail leads to significant change in the survival rates of the experiment groups in comparison with control groups. Also, there is no significant difference between adjuvants to induce immune responses.
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http://dx.doi.org/10.1007/s00436-012-2852-7DOI Listing
July 2012