Publications by authors named "Ebrahim Zabihi"

52 Publications

Therapeutic effect of cold atmospheric plasma and its combination with radiation as a novel approach on inhibiting cervical cancer cell growth (HeLa cells).

Bioorg Chem 2021 Apr 7;111:104892. Epub 2021 Apr 7.

Cancer Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran. Electronic address:

Cervical cancer is one of the important cancers in women. Research on novel treatment approach can reduce the mortality and burden. Although radiotherapy is a common treatment, its negative side effects have concerned physician. In our study, we studied impact of cold atmospheric pressure plasma on the Hela cancer cells, as an alternative treatment. The effect of three different types of such plasma; dielectric barrier discharge (DBD), plasma jet, and afterglow plasma, on the cancer cells were studied. Moreover, some effective operating parameters such as exposure time, applied voltage, composition of working gas in plasma treatment were investigated on the survival of the afterglow plasma. Finally, treatments by the afterglow plasma, gamma radiation (1 Gy), and combination of both were compared. Analysis showed that DBD and plasma jet (direct exposure) effectively killed the cancer cells, even by a minimum applied voltage. But a fraction of the cells survived after the exposure of indirect diffused afterglow plasma. In the case of this plasma, we realized that higher applied voltage and exposure time led to less cell viability. Fewer fractions of survival cells were detected in the case of argon afterglow plasma comparing to oxygen afterglow. Cold atmospheric plasma and its combination with radiation therapy showed a significant decrease in viability of the cells, comparing to the radiation alone. Our research showed that plasma and its combination with radiation therapy have superiority over radiation therapy.
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http://dx.doi.org/10.1016/j.bioorg.2021.104892DOI Listing
April 2021

The Effects of Pre-Treatment and Post-Treatment of Thymol against tert-Butyl Hydroperoxide (t-BHP) Cytotoxicity in MCF-7 Cell Line and Fibroblast Derived Foreskin.

Rep Biochem Mol Biol 2020 Oct;9(3):338-347

Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I. R. Iran.

Background: Some recent studies have reported anti-tumor activity for Thymol, but the findings are inconsistent. This study aimed to investigate and compare Thymol's effects on MCF-7 cancer cells and fibroblasts while treated with tert-Butyl hydroperoxide (t-BHP).

Methods: In the pre-treatment, MCF-7 and fibroblast cells were treated with various Thymol concentrations and incubated for 24 h. Then, t-BHP was added to a final concentration of 50 μM, and the cells were incubated for one h. In the post-treatment, cells were incubated first with 50 μM t-BHP for one h and then treated with Thymol. Cell viability was tested by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Thymol's antioxidant capacity was measured by DPPH and FRAP assays, and lipid peroxidation levels were determined by the TBARS method.

Results: The thymol effects were dose-dependent, and despite their antioxidant properties, at concentrations of 100 µg/ml or more, increased t-BHP toxicity and reduced cancer cell viability. MTT assay result showed that pre-treatment and post-treatment with Thymol for 24 hours effectively reduced MCF-7 and fibroblast cell viability compared with the untreated control group. Both pre- and post-treatment of Thymol, normal fibroblast cell viability was significantly greater than that of the MCF-7 cells.

Conclusion: Our finding showed that Thymol appears to be toxic to MCF-7 cells at lower concentrations than fibroblasts after 24 hours of incubation. Pre-treatment with Thymol neutralized the oxidative effect of t-BHP in fibroblasts but was toxic for MCF-7 cells.
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http://dx.doi.org/10.29252/rbmb.9.3.338DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816789PMC
October 2020

Quinazolinone-dihydropyrano[3,2-b]pyran hybrids as new α-glucosidase inhibitors: Design, synthesis, enzymatic inhibition, docking study and prediction of pharmacokinetic.

Bioorg Chem 2021 Apr 8;109:104703. Epub 2021 Feb 8.

Nano Alvand Company, Avicenna Tech Park, Tehran University of Medical Sciences, Tehran, Iran. Electronic address:

A series of new quinazolinone-dihydropyrano[3,2-b]pyran derivatives 10A-L were synthesized by simple chemical reactions and were investigated for inhibitory activities against α-glucosidase and α-amylase. New synthesized compounds showed high α-glucosidase inhibition effects in comparison to the standard drug acarbose and were inactive against α-amylase. Among them, the most potent compound was compound 10L (IC value = 40.1 ± 0.6 µM) with inhibitory activity around 18.75-fold more than acarboase (IC value = 750.0 ± 12.5 µM). This compound was a competitive inhibitor into α-glucosidase. Our obtained experimental results were confirmed by docking studies. Furthermore, the cytotoxicity of the most potent compounds 10L, 10G, and 10N against normal fibroblast cells and in silico druglikeness, ADME, and toxicity prediction of these compounds were also evaluated.
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http://dx.doi.org/10.1016/j.bioorg.2021.104703DOI Listing
April 2021

Combined Effects of Radiofrequency Electromagnetic Fields and X-Ray in Renal Tissue and Function.

Res Rep Urol 2020 29;12:527-532. Epub 2020 Oct 29.

Department of Medical Physics Radiobiology and Radiation Protection, School of Medicine, Babol University of Medical Sciences, Babol, Iran.

Purpose: Biochemical and histopathological properties of renal tissues were reported to be affected by both radiofrequency electromagnetic fields (RF-EMF) and ionizing radiation. The radiation-induced changes in the kidney, including the serum levels of blood urea nitrogen (BUN) and creatinine (Cr), could lead to adverse health outcomes such as chronic kidney disease. These complications signify the importance of the research in this field. Thus, in this study, the effects of ionizing and non-ionizing radiations, as well as their combination, were assessed by evaluating the alteration in BUN, Cr, and histopathological changes in kidney tissue.

Materials And Methods: Ninety-six male Wistar rats were randomly divided into six groups and were exposed to either 900/1800MHz (mobile phone) or 2.4 GHz RF-EMF (Wi-Fi) radiation for 14 days, 8Gy x-ray, or their combination. Sera were collected from 2 mL of rat blood, then BUN and Cr levels were determined. Also, renal samples were stained with hematoxylin and eosin and evaluated histopathologically.

Results: Both BUN and Cr levels raised non-significantly after exposure to 8 Gy x-rays. Moreover, all measurements in the samples of x-ray groups were in borderline or higher than normal values. The BUN levels of control, Wi-Fi, x-ray, and Wi-Fi+x-ray groups were not significantly different. However, Cr levels in the Wi-Fi group were significantly higher than those of the controls, and BUN to Cr ratio levels were significantly lower than those of the controls. Also, tubular atrophy and vessel wall thickening were associated with these exposures.

Conclusion: Exposure to 900/1800MHz, 2400 MHz EMF can alter the kidney function. However, pre-exposure to 900/1800MHz EMF could modulate the acute adverse effects of lethal x-ray dose, which addresses the adaptive response in the kidney.
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http://dx.doi.org/10.2147/RRU.S257365DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7605663PMC
October 2020

Assessment of function, histopathological changes, and oxidative stress in liver tissue due to ionizing and non-ionizing radiations.

Caspian J Intern Med 2020 May;11(3):315-323

Cancer Research Center, Health Research Institute, Babol University of Medical Sciences, Babol Iran.

Background: Compared to past decades, humans are exposed to rapidly increasing levels of radiofrequency electromagnetic radiations (RF-EMF). Despite numerous studies, the biological effects of human exposure to different levels of RF-EMF are not fully understood yet. This study aimed to evaluate the bioeffects of exposure to "900/1800 MHz" and "2.4 GHz" RF-EMFs, and x-rays alone as well as their potential interactions, i.e. inducing simple additive, adaptive, or synergistic effects.

Methods: 120 Wistar rats were randomly divided into ten groups of 12 each. The rats were exposed to RF-EMF, 10 cGy, and 8 Gy x-rays, a combination of these exposures, or only sham-exposed. The levels of liver enzymes were determined in serum samples by an auto-analyzer. Moreover, the histopathological changes, and the levels of malondialdehyde (MDA), nitric oxide, ferric reducing antioxidant power, total thiols, and protein carbonyl (PCO) were measured.

Results: Among the markers of liver function, gamma-glutamyltransferase was not associated with irradiation but, aspartate transaminase, alanine transaminase, and alkaline phosphatase showed some levels of association. MDA and PCO levels after 8 Gy irradiation increased, but pre-exposure to RF-EMF could modulate their changes. At the cellular level, the frequency of lobular inflammation was associated with the type of intervention.

Conclusion: The exposure to both ionizing and non-ionizing radiations could alter some liver function tests. A short term pre-exposure to RF-EMF before exposure to an 8 Gy challenging dose of x-rays caused the alterations in oxidative stress markers and liver function tests, which indicate that oxidative stress is possibly involved in the adaptive response.
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http://dx.doi.org/10.22088/cjim.11.3.315DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7442457PMC
May 2020

Decrease of intracellular ROS by arbutin is associated with apoptosis induction and downregulation of IL-1β and TNF-α in LNCaP; prostate cancer.

J Food Biochem 2020 09 2;44(9):e13360. Epub 2020 Jul 2.

Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

Increased reactive oxygen species (ROS) along with inflammation are involved in the prostate cancer (PCa). Therefore, this study was conducted to investigate the molecular mechanisms that were affected by arbutin as an antioxidant on prostate cancer cell line; LNCap. The intracellular ROS measurement confirmed that arbutin significantly (p < .05) decreased the ROS levels in a dose-dependent manner. Detection of cell death profile established that 1,000 μM of arbutin could remarkably induced apoptosis (p < .05), while tert-butyl hydroperoxide (tBHP) as ROS inducer prompted necrosis. In addition, 1,000 µM of arbutin successfully decreased expressions of IL-1β and TNF-α genes (p < .05). Furthermore, evaluation of the IL-1β protein level showed that arbutin could significantly decrease this cytokine (p < .05). In summary, reduction of ROS along with increasing apoptosis and decreasing expression of pro-inflammatory genes following arbutin treatment can open new visions in the treatment of prostate cancer using complementary medicine. PRACTICAL APPLICATIONS: Nowadays, arbutin as a glycosylated hydroquinone is available commercially in both natural and synthetic forms. Arbutin is of interest because of its skin-lightening effect, and used in cosmetic products for cutaneous hyperpigmentation. Arbutin inhibited tyrosinase in melanocytes competitively. Moreover, arbutin was able to attenuate oxidative stress and, its anti-inflammatory activities has been established. In addition, arbutin has represented useful activities for suppression of malignant melanoma development. In addition, arbutin exhibits several pharmacological effects, including antimicrobial, antihyperlipidemic, antihyperglycemic, and alpha amylase inhibitory effects. In this study, we showed its effect on prostate cancer in vitro. Therefore, it opens new insights in the complementary medicine that can maintain or improve human health.
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http://dx.doi.org/10.1111/jfbc.13360DOI Listing
September 2020

Systemic effects of starved fibroblast culture supernatant on immunosuppressed rats treated with cancer stem cells (LA7).

Caspian J Intern Med 2020 ;11(2):135-142

Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

Background: The present study aimed to investigate and compare the effect of starved fibroblast culture supernatant (SFS), DMEM and normal saline alone or along with LA7 on dexamethasone-treated immunosuppressed Wistar rats.

Methods: After the isolation of fibroblasts from the fresh foreskin of children, it was cultured in serum-free DMEM, and the supernatant collected after 16 hours (16h-SFS). This solution and the other treatments were injected subcutaneously into the rats from each group once daily for 14 days. The liver, intestine and lung histology along with blood cellular and biochemical characteristics were studied.

Results: The results showed that dexamethasone as immunosuppressant reduced the body weight. The histological change in the liver was mild fibrosis induced by LA7+16h-SFS. Also, among the different blood cellular and biochemical indices measured, the eosinophil percentage in the 16h-SFS treated rats , glucose levels in the 16h-SFS+LA7 group and triglyceride concentrations in the 16h-SFS group were changed (p<0.05).

Conclusion: This study showed that the secretions of starved fibroblasts especially that combined with LA7 cancer stem cells could induce some minor histological and biochemical changes in immunosuppressed rats, and also it opened a new window for subsequent investigations on unknown mechanisms related to this work.
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http://dx.doi.org/10.22088/cjim.11.2.135DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7265514PMC
January 2020

The Effect of Arbutin on The Expression of Tumor Suppressor , Ratio and Oxidative Stress Induced by Tert-Butyl Hydroperoxide in Fibroblast and LNcap Cell Lines.

Cell J 2021 Jan 22;22(4):532-541. Epub 2020 Apr 22.

Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Objective: Arbutin (p-hydroxyphenyl-β-D-glucopyranoside) possesses beneficial functions including antioxidant, antiinflammatory, and anti-tumoral activities. Due to the important role of oxidative stress and apoptosis in the successful treatment of cancer, understanding mechanisms that lead to apoptosis in cancer cells, is essential. The purpose of the current study was to evaluate the effect of arbutin on tert-butyl hydroperoxide (t-BHP)-induced oxidative stress and the related mechanisms in fibroblast and Lymph Node Carcinoma of the Prostate (LNCaP) cells.

Materials And Methods: In this experimental study, the LNCaP and fibroblast cell lines were pre-treated with arbutin (50, 250 and 1000 μM). After 24 hours, t-BHP (30 and 35 μM) was added to the cells. Viability was measured (at 24 and 48 hours) using MTT assay. The antioxidant effect of arbutin was measured by FRAP assay. The mRNA expression of and ratio were measured using quantitative polymerase chain reaction (PCR). The percentage of apoptotic or necrotic cells was determined using a double staining annexin V fluorescein isothiocyanate (FITC) apoptosis detection kit.

Results: Arbutin pre-treatment increased the total antioxidative power and cell viability in the MTT assay and reduced ratio, mRNA expression and necrosis in fibroblasts exposed to the oxidative agent (P<0.001). In addition, our results showed that arbutin can decrease cell viability, induce apoptosis and increase ratio in LNCaP cells at some specific concentrations (P<0.001).

Conclusion: Arbutin as a potential functional β-D-glucopyranoside has strong ability to selectively protect fibroblasts against t-BHP-induced cell damage and induce apoptosis in LNCaP cells.
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http://dx.doi.org/10.22074/cellj.2021.6902DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211292PMC
January 2021

Targeting LA7 breast cancer stem cells of rat through repressing the genes of stemness-related transcription factors using three different biological fluids.

Gene 2020 Apr 21;734:144381. Epub 2020 Jan 21.

Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran. Electronic address:

Down-regulation of stemness genes expression is important in differentiation therapy against cancer stem cells (CSCs). The aim of this study was to evaluate the Oct4 , Sox2, Nanog, and C-myc expression in rat breast cancer stem cells (LA7) which treated with human ovarian follicular fluid (FF), replicative senescent fibroblast culture supernatant (P14), and 16 h serum starved fibroblast supernatant (16 h-SFS). The cells were exposed to these biological fluids for 24 h, 72 h, and 7 days. Stem-loop RT-qPCR assay was used to quantify the expression of above mentioned genes. Results showed that FF had the least cytotoxic effect on the LA7 cells. Except for Nanog gene, exposure of LA7 cell line to 16 h-SFS and P14 decreased significantly expression of the three other genes after 24 h (P < 0.05). Nanog and Sox2 genes expression was also decreased in LA7 cells which have been already treated with FF for 24 h. Moreover, compared to the control solution, the expression of Oct4 increased significantly after 7 days exposure to FF (P < 0.05). Annexin V-PE /7-AAD-, acridine orange/ethidium bromide staining and doubling time assays revealed apoptosis and necrosis induction by these biological fluids in LA7 cells. Moreover, in an in vitro model of metastasis assay, i.e., scratch test, these fluids exhibited anti-LA7 migration activity which culminated in 16 h-SFS treated cells. Generally, this study showed that FF, 16 h-SFS, and P14 have positive effects on down-regulation of Nanog, Oct4, Sox2 and C-myc expression, and consequently can increase the differentiation of breast cancer stem cells. For the first time, this study provided some evidence indicating that some biological fluids have potential to differentiate the CSCs, show anti- survival, growth-, and cell migration activity.
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http://dx.doi.org/10.1016/j.gene.2020.144381DOI Listing
April 2020

A comparative study on immunophenotypic characterization and osteogenic differentiation of human mesenchymal stromal cells derived from periodontal ligament and gingiva.

J Periodontol 2020 09 17;91(9):1194-1202. Epub 2020 Jul 17.

Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

Background: Mesenchymal stem cells (MSCs) derived from periodontal ligament (PDL) and gingiva can be used for the development of cell-based regenerative approaches in dentistry and medicine. The purpose of this investigation was to establish a method for isolation of human stem cells from the PDL and gingiva, multilineage differentiation of those cells, and comparison of periodontal ligament mesenchymal stem cells (PDLMSCs) and gingival mesenchymal stem cells (GMSCs).

Methods: PDL and gingival tissues of third molar teeth were digested enzymatically and the proliferative potential of human PDLMSCs and GMSCs was compared by MTT assay. The expression of cell surface epitopes was analyzed by flow cytometry. To investigate the multilineage differentiation capacity of these stem cells, osteogenic and adipogenic differentiation was achieved. The specific staining of nodules was performed to evaluate differentiation, whereas the expression of alkalin phosphatase (ALP) and collagen A I (COL I) genes was analyzed by quantitative real-time polymerase chain reaction.

Results: The outgrown cells derived from PDL and gingival tissues were similar, fibroblast-like, and spindle-shaped. Further, the proliferation potential of GMSCs was greater than PDLMSCs. Both types of stem cells expressed MSC precursor markers, including CD73, CD90, and CD105, whereas they were negative for hematopoietic markers, including CD34 and CD45. PDLMSCs demonstrated more osteogenic potential compared to GMSCs with strong mineral noduls, and significantly greater expression of up-regulated bone-related markers ALP and COL I.

Conclusion: MSCs derived from PDL and gingiva demonstrated multipotent characteristics, suggesting new therapeutic approaches in tissue engineering and PDLMSCs are more appropriate candidates for this purpose.
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http://dx.doi.org/10.1002/JPER.19-0535DOI Listing
September 2020

Green synthesis, formulation and biological evaluation of a novel ZnO nanocarrier loaded with paclitaxel as drug delivery system on MCF-7 cell line.

Colloids Surf B Biointerfaces 2020 Feb 30;186:110686. Epub 2019 Nov 30.

Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran; Department of Pharmacology & Toxicology, School of Medicine, Babol University of Medical Sciences, Babol, Iran.

In this study we design green synthesis of a novel ZnO nanocarrier loaded with paclitaxel as a drug delivery system with high cytotoxicity against breast cancer cell line (MCF-7) and low side effects on the normal cell line (fibroblast). Paclitaxel is formulated in high concentration in Cremophor EL because of its low solubility. Zinc oxide nanoparticles (ZnO NPs) were prepared by the ethanolic extract of Camellia sinensis L., then coated with chitosan (Ch) and loaded with paclitaxel (PTX) to improve drug delivery. The physicochemical properties were observed by transmission electron microscopy (TEM), thermo-gravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), and Fourier transform infrared spectroscopy (FTIR). Drug loading on ZnO-Ch NPs was measured by high performance liquid chromatography (HPLC). In vitro apoptosis assay was assessed by flow cytometry. The cytotoxic effect of the nanocarrier drug was investigated using MTT assay in cancerous and normal cell lines. The PTX-loaded ZnO-Ch NPs showed cytotoxic effects on MCF-7 cells, with minimal detrimental effects on normal fibroblasts. The results of apoptosis assay were compliant with MTT findings. Generally, ZnO-Ch NPs could be used as a promising drug delivery platform for PTX with low side effect on normal cell line and high cytotoxic effect on breast cancer cell line.
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http://dx.doi.org/10.1016/j.colsurfb.2019.110686DOI Listing
February 2020

Anticancer properties of chitosan against osteosarcoma, breast cancer and cervical cancer cell lines.

Caspian J Intern Med 2019 ;10(4):439-446

Dental Materials Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

Background: Cancer is still the most common cause of morbidity in the world. Chitosan, a commonly used natural polymer, is consisted of different molecular weight with different biological activities.The purpose of this study was to determine cytotoxicity effect of high molecular weight (HMWC) and low molecular weight of chitosan (LMWC) on three cancerous cell lines MCF-7, HeLa and Saos-2 with different histological origin.

Methods: The anticancer property of two types of chitosan on three cancerous cell lines and human fibroblast as normal cell line, was evaluated by cytotoxic activity including their apoptosis induction properties. Chitosan solutions 2% (w/v) were prepared. The cells were treated by different concentration of chitosan and viability was determined by MTT assay after 24, 48 and 72 h .Also the mode of cell death-apoptosis vs necrosis ,was determined by Annexin V staining assay and analyzed by flow cytometry.

Results: While both types of chitosan were effective in inhibiting cell proliferation of three cancerous cell lines, fibroblast cells showed somehow more compatibility with chitosan. Despite of a significant decrease in all 3 cell lines viability, up to 90%, but we didn't see a concentration dependent difference between both types of chitosan (HMWC and LMWC) in their cytotoxic effects. Flow cytometry analysis showed necrosis more observable with MCF7 while the apoptosis pattern of death was more in Saos-2 and HeLa. Also, higher viability with both types of chitosan was seen in fibroblast as normal cells.

Conclusion: While chitosan is compatible with normal diploid fibroblast cells, it shows anticancerous effect against 3 cancerous cell lines. Furthermore, it seems that the molecular weight of chitosan does not affect its anticancerous property.
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http://dx.doi.org/10.22088/cjim.10.4.439DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6856915PMC
January 2019

Infertility as a risk factor for breast cancer: Results from a hospital-based case-control study.

J Cancer Res Ther 2019 Jul-Sep;15(5):976-980

Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Science, Babol, Iran.

Context: Breast cancer is the most common cancer in women worldwide and its prevalence is increasing.

Aims: The aim of this study was to investigate the extent to which a history of infertility can present as risk factors for breast cancer.

Settings And Design: This was a hospital-based case-control study.

Subjects And Methods: In this study, 1177 women with breast cancer were participated for assessing the risk for this cancer. The control was 1204 women with self-reported free-cancer history who were matched in terms of age and residence.

Statistical Analysis Used: Using logistic regression, it was examined whether infertility is a risk factor of case-control status in addition to marriage age, menarche age, body mass index, number of pregnancies, family history of breast cancer, and previous oral contraceptive use. The data were considered significant at P ≤ 0.05.

Results: Infertility history was reported in 12.5% (n = 147) of the cases and 5.8% (n = 70) of the controls. Infertility history was a relevant risk for case-control study in addition to other risk factors (odds ratio: 2.43; 95% confidence interval, 3.34-1.77).

Conclusions: This study found that infertility may be as the main risk factor for breast cancer in Iranian women, therefore, doing breast screening in women who have one or more risk factors, must receive more emphasis.
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http://dx.doi.org/10.4103/jcrt.JCRT_905_16DOI Listing
July 2020

Iranian propolis efficiently inhibits growth of oral streptococci and cancer cell lines.

BMC Complement Altern Med 2019 Oct 11;19(1):266. Epub 2019 Oct 11.

Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

Background: Propolis is a natural bee product with a wide range of biological activities that are related to its chemical composition. The present study investigated the quantification of quercetin (Q) in Ardabil ethanol extract of propolis (AEEP), and then compared its anti-bacterial, anti- biofilm and cytotoxic effects on cancer and normal cell lines.

Method: In the present study, the chemical composition of AEEP was determined through the high-performance liquid chromatography (HPLC). The AEEP and its main component, quercetin (Q), were evaluated in vitro against 57 oral streptococci by a broth micro-dilution method. The biofilm formation was assessed through the crystal violet staining and MTT assays. The impact of AEEP and Q anti-proliferative effect were evaluated on the fibroblast as normal and cancer cell lines (KB and A431).

Results: The Q concentration in the composition of AEEP was 6.9% of all its components. The findings indicated that the AEEP and Q were efficient against the cariogenic bacteria and were able to inhibit the S.mutans biofilm adherence at a sub-MIC concentration. Moreover, electron micrographs indicated the inhibition of biofilms compared to control biofilms. In addition, the AEEP and Q indicated a dose-dependent cytotoxic effect on A431 and KB cell lines. On the contrary, they had no cytotoxic effect on fibroblast cells.

Conclusion: The results indicated that the synergistic impact of main components of AEEP was related to the inhibition of the cancer cell proliferation, cariogenic bacteria and oral biofilm formation. It may play a promising role in the complementary medicine and, it is suggested to be used as food additives.
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http://dx.doi.org/10.1186/s12906-019-2677-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6788029PMC
October 2019

The potential use of mesenchymal stem cells for the treatment of multiple sclerosis.

Life Sci 2019 Oct 2;235:116830. Epub 2019 Sep 2.

Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran; Neuroscience Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran. Electronic address:

Multiple sclerosis (MS) is a chronic autoimmune inflammatory disease of the central nervous system (CNS). In attempt to identify an appropriate treatment for improving the neurological symptoms and remyelination process, autologous and allogenic transplantation of mesenchymal stem cells (MSCs) have been introduced as an effective therapeutic strategy in MS. MSCs are a heterogeneous subset of pluripotent non-hematopoietic stromal cells that are isolated from bone marrow, adipose tissue, placenta and other sources. MSCs have considerable therapeutic effects due to their ability in differentiation, migration, immune-modulation and neuroregeneration. To date, numerous experimental and clinical studies demonstrated that MSCs therapy improves the CNS repair and modulates functional neurological symptoms. Here, we provided an overview of the current knowledge about the clinical applications of MSCs in MS. Furthermore, the major challenges and risks of MSCs therapy in MS patients have been elucidated.
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http://dx.doi.org/10.1016/j.lfs.2019.116830DOI Listing
October 2019

Biscoumarin-1,2,3-triazole hybrids as novel anti-diabetic agents: Design, synthesis, in vitro α-glucosidase inhibition, kinetic, and docking studies.

Bioorg Chem 2019 11 16;92:103206. Epub 2019 Aug 16.

Nano Alvand Company, Avicenna Tech Park, Tehran University of Medical Sciences, Tehran 1439955991, Iran. Electronic address:

A novel series of biscoumarin-1,2,3-triazole hybrids 6a-n was prepared and evaluated for α-glucosidase inhibitory potential. All fourteen derivatives exhibited excellent α-glucosidase inhibitory activity with IC values ranging between 13.0 ± 1.5 and 75.5 ± 7.0 µM when compared with the acarbose as standard inhibitor (IC = 750.0 ± 12.0 µM). Among the synthesized compounds, compounds 6c (IC = 13.0 ± 1.5 µM) and 6g (IC = 16.4 ± 1.7 µM) exhibited the highest inhibitory activity against α-glucosidase and were non-cytotoxic towards normal fibroblast cells. Kinetic study revealed that compound 6c inhibits the α-glucosidase in a competitive mode. Furthermore, molecular docking investigation was performed to find interaction modes of the biscoumarin-1,2,3-triazole derivatives.
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http://dx.doi.org/10.1016/j.bioorg.2019.103206DOI Listing
November 2019

Antibacterial activity of high-molecular-weight and low-molecular-weight chitosan upon oral pathogens.

J Conserv Dent 2019 Mar-Apr;22(2):169-174

Social Determinants of Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

Context: One of the common oral bacterial infectious diseases is dental caries. Control of dental plaque formed by leads to prevention and treatment of caries. Chitosan (1-4, 2-amino-2-deoxy-b-D-glucan), a deacetylated derivative from chitin, is an antimicrobial polysaccharide that exerts broad-spectrum activity against pathogenic bacteria and has been suggested as a preventive and therapeutic material for dental caries.

Aim: The aim of this investigation is whether chitosan has effective antimicrobial and antibiofilm properties against common cariogenic microorganisms.

Materials And Methods: The effect of 0.019-5 mg/ml of high-molecular-weight (HMW) and low-molecular-weight (LMW) chitosan on and was evaluated, and minimal inhibitory concentration (MIC) and minimal bactericide concentration (MBC) were determined. In addition, the effects of HMW and LMW of chitosan on bacterial adhesion to surfaces and biofilm formation were assayed by tube method.

Results: The results showed that chitosan is capable of inhibiting and growth ( = 0.001). MIC of HMW chitosan for and was 0.62 mg/mL and MIC of LMW chitosan for and was 0.62 mg/mL, 1.25 mg/mL, respectively. MBC of HMW chitosan for and was 1.25 mg/mL, respectively, and MBC of LMW chitosan for and was 1.25 and 2.5 mg/ml, respectively. On the other hand, HMW chitosan was more effective than LMW chitosan. In addition, showed equal MIC and MBC values for both MWs chitosan, but was more resistant to LMW chitosan. Regarding biofilm growth, chitosan inhibited and adhesion and biofilm formation. The results of tube test showed weak adherence and biofilm formation in concentration of 0.312 and 0.625 mg/ml, but 1.25 and 2.5 mg/ml concentrations of both MWs could completely inhibit biofilm formation.

Conclusion: These results display the potential of chitosan to be used as an effective antibacterial and antibiofilm agent for oral hygiene and health care.
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http://dx.doi.org/10.4103/JCD.JCD_300_18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6519183PMC
May 2019

Immunotoxicity of estrogen and nonylphenol on apoptosis and expression of ERs in goldfish macrophage: Opening new avenue for discovering the role of experimental model systems and sexes.

Aquat Toxicol 2019 Apr 13;209:159-167. Epub 2019 Feb 13.

Department of Fisheries, Faculty of Marine Natural resources, Khorramshahr University of Marine Science and Technology, P.O. Box 669, Khorramshahr, Iran. Electronic address:

The expression of estrogen receptors (ERs) and their roles in important cell processes such as apoptosis in the macrophages exposed to estrogen/xenoestrogen have remained a complex secret. This study focused on the expression of estrogen receptors (ERs) and the stimulation of apoptosis in the macrophages from the two sexes of goldfish (Carassius auratus) exposed to 17-βestradiol (E2) and nonylphenol (NP) under in vivo and in vitro conditions. For the in vivo experiment, fish were exposed to NP (10 M and 10 M) and E2 (10 M) for 24 days. Then, the head kidney macrophages from the male and the female goldfish were isolated and assayed. For the in vitro experiments, the macrophages derived from the two sexes were cultured in L-15 medium and exposed to E2 (150 nM) and NP (10 nM and 150 nM) for 3 days. The results showed that the three isoforms of ERs (ERα, ERβ, ERβ) were expressed in the goldfish macrophages. After the exposure of macrophages to NP and E2, sex-specific increase of ERs expression and apoptosis were observed (P < 0.05). The expression of ERα after NP treatment showed the highest alteration, with the response being concentration-dependent. The most alteration of ERs expression were observed in the in vivo experiment. This study provides insight to understand how exposure of the goldfish macrophages to E2 and NP can up-regulate the transcript levels of estrogen receptor subtypes and stimulate apoptosis.
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http://dx.doi.org/10.1016/j.aquatox.2019.02.007DOI Listing
April 2019

Spontaneously contracting cell aggregates derived from grass carp heart as a promising tool in in vitro heart research.

Cytotechnology 2019 Feb 1;71(1):261-266. Epub 2019 Jan 1.

Department of Marine Biology, Faculty of Marine Sciences, Khorramshahr University of Marine Science and Technology, P.O. Box 669, Khorramshahr, Iran.

Regarding challenges in isolation and maintenance of cultured heart cells, introduction of new in vitro heart model that is stable and exhibits long-term spontaneously contracting cell aggregates (SCCs), whose electrophysiological properties are comparable to the human heart, is required. This research aimed to establish cardiac primary cells and to evaluate the effects of culture conditions. Also the effect of fish age on beating SCC and cardiac cell morphology were studied. Twelve healthy grass carps (Ctenopharyngodon idella) were divided into four groups based on their age. Non-enzymatic explant culture was used and heart explants were incubated at 21-31 °C for 60 days. After proliferation of the cardiac primary cells, changes in their morphology and their beatings were recorded. The results showed that the explants derived from different age of grass carp fish are fully viable and proliferative with formation of SCC for a long period of time. By increasing the number of adhered cells, the cardiac primary cells became more flat and elongated. Increasing the medium temperature and fetal bovine serum concentration in culture medium led to decline and enhancement in beat frequencies of heart cell aggregates, respectively. Also, grass carp heart explant had high potential in regeneration (especially in young fish) and thus high feasibility to generate stable long-term cultures. In general, it seems that explant culture of heart from grass carp can be considered as a promising tool in heart research area.
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http://dx.doi.org/10.1007/s10616-018-0281-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368527PMC
February 2019

The assessment of radio-adaptive response in graves' hyperthyroidism patients following radioactive iodine uptake.

World J Nucl Med 2018 Oct-Dec;17(4):270-274

Department of Medical Physics Radiobiology and Radiation Protection, Babol University of Medical Sciences, Babol, Iran.

Low doses of radiation affect the response of cells to higher doses; this phenomenon is called radio-adaptive response, which leads to increased resistance to subsequent higher doses. We have investigated the adaptive response using 0.37 MBq priming dose of I-131 followed by 296-444 MBq challenging dose in peripheral human lymphocyte cells. The study was performed on 42 patients with Graves' disease and 29 healthy adult persons as a control group. The patients were divided into two groups. In the first group, patients were referred for radioactive iodine therapy with a specific dose, and iodine was given to them on the day of referral. In the second group, patients were referred for radioactive iodine uptake and radioactive iodine therapy, and iodine uptake was initially performed, then 24 h later, iodine therapy was done. In both groups, 1 month after treatment, blood samples were taken to cytokinesis-block micronucleus (MN) assay. The number of MN in binuclear lymphocyte cells was counted as an end point test. The mean frequency of MN in first, second, and control groups was 75.86 ± 12.68, 71.45 ± 12.56, and 20.06 ± 7.30, respectively. Our results showed that the frequency of total chromosome aberration in both radiation groups was higher than controls. However, in the first group was higher than another group, but their difference was not statistically significant. According to the results, we cannot approve the hypothesis that 0.37 MBq I-131 administration before iodine therapy could induce a radio-adaptive response in lymphocytes of Graves' patients.
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http://dx.doi.org/10.4103/wjnm.WJNM_83_17DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6216742PMC
December 2018

Interruption of immune responses in primary macrophages exposed to nonylphenol provides insights into the role of ER and NF-KB in immunotoxicity of Persian sturgeon.

Fish Shellfish Immunol 2019 Mar 16;86:125-134. Epub 2018 Nov 16.

Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

The severe decline in population of sturgeons due to pollution highlighted poor understanding about the immunotoxicological responses of sturgeons. This study was designed in three experiments to find out how nonylphenol (NP) interrupts some pro-inflammatory immune parameters in macrophages from Persian sturgeon (Acipencer persicous) as the oldest vertebrate model conserving intact innate immune system. After determination of IC values of NP (200 μM), some pro-inflammatory immune parameters and induced apoptosis in macrophages at low dose (10 nM) and high dose (100 nM) of NP and of 17β estradiol (E2) (positive control) were determined after 6, 24 and 48 h of the exposure (as the first experiment). The two doses of NP induced pro-inflammatory reaction and apoptosis with strong correlations, whereas this result was observed more obviously in high dose of E2. In the second experiments, the macrophages were exposed to the two doses of NP along with estrogen receptor alpha (ERα) antagonist, which consequently decreased the induction of pro-inflammatory reactions. Similarly, in the third experiment, NF-KB and ERα antagonists were used and pro-inflammatory reactions decreased compared to the control group (P < 0.05). Decreasing correlation between immune parameters following the second and third experiments verified interaction between ERα and NF-KB pathways. Thus, NP could be immune disrupter and apoptosis inducer in sturgeon macrophages in vitro, even in low dose. For the first time, this study revealed that NP can induce pro-inflammatory reactions in macrophages derived from sturgeons.
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http://dx.doi.org/10.1016/j.fsi.2018.11.038DOI Listing
March 2019

Optimization of macrophage isolation from the Persian sturgeon and the Caspian kutum fish: a comparative study.

Cytotechnology 2018 Dec 10;70(6):1643-1654. Epub 2018 Oct 10.

Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

The aim of this research was a comparative study on the isolation and culture of head kidney macrophages derived from Acipenser persicous and Rutilus frisii kutum as teleost and chondrostei species of fish. The macrophages were isolated by density gradient sedimentation, followed by adherence to a plastic surface. They exhibited strong phagocytic activity against bacteria. The effect of cell density, incubation time, FBS percentage, pH and temperatures on the cell number and viability were determined and compared. Also, the effect of light/dark regimen on viability, adherence, release of reactive oxygen species (ROS) and nitric oxide (NO) in the macrophages was determined. The results showed that the Caspian kutum macrophages were more sensitive to FBS percentage and cell density whereas the Persian sturgeon macrophages were more sensitive to pH of the cell culture media. The adherence and viability of the macrophages from both fish species firstly increased (P < 0.05) after exposure to a light/dark regimen, but then significantly decreased as did ROS and NO productions. For the first time, this study has determined the optimal conditions for primary culture of macrophages derived from sturgeons, and shows the unique effect of light on the biology of fish immune cells.
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http://dx.doi.org/10.1007/s10616-018-0256-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269369PMC
December 2018

Nanostructured akermanite glass-ceramic coating on Ti6Al4V for orthopedic applications.

J Appl Biomater Funct Mater 2019 Apr-Jun;17(2):2280800018793819. Epub 2018 Aug 24.

4 Department of Mechanical Engineering, Politecnico di Milano, Milan, Italy.

Glass ceramics are widely used to enhance the functionality of inert metallic materials typically used for hard-tissue engineering. Biofunctionality of glass ceramics can in turn be significantly boosted with the addition of trace element dopants. Herein, we synthesized a nanostructured glass ceramic and used magnesium (Mg), which is known to promote osteoblast adhesion and proliferation, for further functionalization. The nanostructured akermanite glass ceramic (CaMgSiO) was used to coat Ti6Al4V substrates by the sol-gel method. Scanning and transmission electron microscopy as well as X-ray diffraction were used to assess the structural morphology and phase composition of the coating, respectively. The micrographs showed a uniform and crack-free coating structure. Atomic force microscopy observation revealed a disordered surface roughness for coated samples. In vitro cytocompatibility tests revealed that Saos-2 cells cultured on bare samples adopted a rounded morphology, whereas cells cultured on the coated samples represented a more spread out configuration and also increased proliferation. The characterizing tests confirmed the efficiency of the synthesis method and the in vitro biocompatibility of the synthesized coating, indicating its suitability to be used for bone implants.
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http://dx.doi.org/10.1177/2280800018793819DOI Listing
December 2019

A comparative study on cytotoxicity and genotoxicity of the hydroxyapatite-bioactive glass and fluorapatite-bioactive glass nanocomposite foams as tissue scaffold for bone repair.

J Biomed Mater Res A 2018 10 8;106(10):2605-2612. Epub 2018 Sep 8.

Department of Biostatistics and Epidemiology, School of Medicine, Babol University of Medical Sciences, Babol, Iran.

Considering to the possibility of cellular and genetic damage by the implant materials in the patient and the clinician, the safety of the biomaterials should be evaluated. The purpose of this study was to compare the cytotoxicity and genotoxicity induced by two nanocomposites, hydroxyapatite/bioactive glass (HA/BG) and fluorapatite/bioactive glass (FA/BG) in vitro. Biomaterial extracts (BMEX, 100%) were prepared by incubating 100 mg/mL of each biomaterial powder in complete culture medium (RPMI1640 + 10% FBS) for 72 h. Saos-II cells were exposed to different concentrations of BMEXs for different periods of time and evaluated at the end of each period. According to 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay results, both BMEXs at low concentrations (<25%) has no inhibitory effects on the cells growth. After 24 h of exposure, only HA/BG BMEX at 100% concentration showed significant cytotoxic effect. After 48 and 72 h, both HA/BG and FA/BG BMEXs showed similar cytotoxic effect at concentration higher than 75 and 50%, respectively. The results of the comet assay showed that the tail elongation, and proportionally DNA damage, increased in a dose/time dependently fashion with BMEXs exposure. Based on low and similar cytotoxicity and genotoxicity profiles on the Saos-II cell line, it could be concluded that FA/BG, like HA/BG, could be a good candidate for further in vivo biocompatibility studies to be used in bone tissue repair. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2605-2612, 2018.
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http://dx.doi.org/10.1002/jbm.a.36452DOI Listing
October 2018

CYP1A gene expression as a basic factor for fipronil toxicity in Caspian kutum fish.

Toxicol Rep 2018 14;5:113-124. Epub 2017 Dec 14.

Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

The aim of this study was to assess the effects of fipronil insecticide on the Caspian kutum fish at different levels of biological organizations and to find possible relationship between these biomarkers. Different doses of fipronil (65, 130 and 200 mg/kg) were intraperitoneally administered to the fish for 2 weeks. After 7 and 14 days of exposure, alterations in organ-somatic index, tissue and DNA structure, oxidative stress and CYP1A gene expression in gill, liver, brain and kidney were studied. Determination of these parameters in the liver showed that the degree of tissue change (DTC), comet tail, superoxide dismutase (SOD) and relative CYP1A mRNA expression increased mostly in a time dependent manner whereas in the kidney increased mostly in a dose dependent manner. These parameters in the gill increased more in time and dose dependent manner. Apart from the changes in CYP1A expression and oxidative stress, no alterations was observed in the brain. Multiple regression analysis showed that the CYP1A had the most correlation with the organ-somatic index (R = 0.76) and comet tail (R = 0.89) in the liver, and with DTC (R = 0.93) and oxidative stress (R = 0.87) in the kidney. Generally, this study showed that CYP1A gene expression can be considered as one basic factor for fipronil toxicity in this fish. However, other possible factors also should be considered for future research.
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http://dx.doi.org/10.1016/j.toxrep.2017.12.014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5978015PMC
December 2017

Effect of MTA and CEM on Mineralization-Associated Gene Expression in Stem Cells Derived from Apical Papilla.

Iran Endod J 2018 ;13(1):94-101

Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

Introduction: This study assessed the effect of mineral trioxide aggregate (MTA) and calcium-enriched mixture (CEM) cement on odontogenic differentiation and mineralization of stem cells.

Methods And Materials: After confirmation of stemness and homogeneity of stem cells derived from apical papilla (SCAPs) using flow cytometry, the cells were exposed for 3 weeks to either osteogenic medium (OS) or CEM extract+OS (CEM+OS) or MTA extract in OS (MTA+OS) or DMEM based regular culture media (negative control). Relative expression of alkaline phosphatase (ALP), dentine sialophosphoprotein (DSPP), osteocalcin (OSC), and osterix (SP7) were measured at days 14 and 21 using RT-qPCR method. At the same time points Alizarin Red staining method was used to assess mineralization potential of SCAPS. Gene expression changes analysis were made automatically using REST® software and a <0.05 was considered significant.

Results: After 2 weeks of exposure, expression of all genes were between 3 and 52 times the expression of GADPH (all were upregulated except SP7 in the control, <0.05). After 3 weeks, relative expressions of the genes: ALP, SP7, DSPP, and OSC were respectively 275.9, 528.3, 98.4, and 603.7 times the expression of GADPH in the control group (OS). These were respectively 17.405, 29.2, 11.8, and 6.5 in CEM+OS group, and 163.8, 119.7, 102.5, and 723.9 in MTA+OS group. All of these were confirmed as upregulated (<0.05) except for ALP and OSC of DM+CEM group. After 2 weeks, alizarin red staining showed similar mineralized nodules in OS, MTA+OS, and CEM+OS. In third week, larger nodules were seen in MTA+OS and OS, but not in CEM+OS.

Conclusion: After 2 weeks, gene expressions were almost comparable in OS, CEM+OS, and MTA+OS. After 3 weeks, OS and MTA+OS upregulated genes much greater than in 2nd week. However, upregulation in CEM+OS might not increase in 3rd week compared to those in 2nd week.
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http://dx.doi.org/10.22037/iej.v13i1.17860DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5800449PMC
January 2018

Comparison of waterborne and intraperitoneal exposure to fipronil in the Caspian white fish ( on acute toxicity and histopathology.

Toxicol Rep 2017 23;4:348-357. Epub 2017 Jun 23.

Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

Fipronil is an effective insecticide widely used in agriculture with potential ecotoxicological consequences. The median lethal dose (LD) and concentration (LC) of fipronil in 16.3 g Caspian white fish, fingerlings were determined. To determine the LD, a total of 133 fish were assigned to 19 tanks (7 fish/tank) including one control and 6 treatment groups (300, 450, 550, 650, 750, 850 mg/kg). Fish were injected intraperitoneally and monitored at 96 h. The LD of fipronil was 632 mg/kg suggesting it was slightly toxic to the Caspian white fish. To determine LC, 114 fish were assigned to 19 tanks (6 fish/tank) including one control and 6 treatment groups (300, 400, 500, 600, 700, 800 μg/L). The LC of fipronil was 572 μg/L, which was highly toxic to the fish. The degree of tissue change (DTC) in vital organs from moribund fish exposed via waterborne exposure showed severe damage (DTC: 71 ± 52 for 700 μg/L) in the gill, including aneurisms, extensive fusion and necrosis. The fish exposed through the intraperitoneal route seemed to have severe lesions (DTC: 66 ± 50 for 750 mg/kg) in the kidney, involving hemorrhage, tubular degeneration and necrosis. The liver had no significant differences in DTC values between the two routes and showed pyknosis and sinusoid dilation. Hematoxylin and eosin staining did not show any histological alterations in the brain but nissl staining showed some alterations in distribution of purkinje cells. Generally, this study showed that the route of exposure to fipronil not only affects its acute toxicity but also determines the main target organs of toxicity and histopathological alterations in Caspian white fish.
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http://dx.doi.org/10.1016/j.toxrep.2017.06.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615146PMC
June 2017

Cytochrome P-450 1B1 Leu432Val Polymorphism Does Not Show Association With Breast Cancer in Northern Iranian Women With a History of Infertility.

Biomark Insights 2017 20;12:1177271916689058. Epub 2017 Feb 20.

Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

The Cytochrome P-4501B1 () Leu432Val polymorphism has been previously shown to be associated with some types of cancer and affects -mediated metabolism of various infertility drugs. To establish the frequency of Leu432Val polymorphism among women with a history of infertility drug use, we studied the genotypes of 147 patients with breast cancer with a history of infertility and 150 cancer-free, infertile women (control group) in Northern Iran. A polymerase chain reaction-based restriction fragment length polymorphism assay was used to detect GG (Val/Val), CG (Leu/Val), and CC (Leu/Leu) genotype frequencies, which did not vary significantly between the 2 patient groups ( = .847). We established for the first time that the incidence of Leu432Val polymorphism is 46.6% among women with infertility history and breast cancer in Northern Iran. Finally, our results do not show any significant association between Leu432Val polymorphism and breast cancer in infertile women in this region, who have also received infertility treatment.
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http://dx.doi.org/10.1177/1177271916689058DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5391986PMC
February 2017

Human serum miR-34a as an indicator of exposure to ionizing radiation.

Radiat Environ Biophys 2016 11 25;55(4):423-429. Epub 2016 Aug 25.

Cellular and molecular biology research center, Health research institute, Babol University of Medical Sciences, Babol, Iran.

Radiation exposure in industrial accidents or nuclear device attacks is a major public health concern. There is an urgent need for markers that rapidly identify people exposed to ionizing radiation (IR). Finding a blood-based marker is advantageous because of the ease of sample collection. This study was designed to test the hypothesis that serum miR-34a could serve as an indicator of exposure to IR. Therefore, 44 women with breast cancer, where radiotherapy was part of their therapeutic protocol, were investigated in this study. After demonstrating the appropriateness of our microRNA (miRNA) extraction efficiency and miRNA assay in human serum, we analyzed the miR-34a level in paired serum samples before and after radiotherapy. Fifty Gy X-ray irradiation in daily dose fractions of 2 Gy, 5 days per week, was used in this study. We demonstrated that IR significantly increased serum level of miR-34a. By measuring miR-34a in serum, we could distinguish irradiated patients with sensitivity of 65 % and specificity of 75 %. According to this study, serum miR-34a has the potential to be used as an indicator of radiation exposure.
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http://dx.doi.org/10.1007/s00411-016-0661-6DOI Listing
November 2016

Effects of Fenton Reaction on Human Serum Albumin: An In Vitro Study.

Electron Physician 2016 Sep 20;8(9):2970-2976. Epub 2016 Sep 20.

Ph.D. of Pharmacology and Toxicology, Associate Professor, Cellular and Molecular Biology Research Center, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.

Introduction: Human serum albumin (HSA) is a critical protein in human blood plasma, which can be highly damaged by oxidative stress. The aim of this study was to analyze modifications of this protein after oxidation using a Fenton system.

Methods: In this 2015 experiment, different ratios of Fenton reagent (Fe2+/HO) was incubated with one concentration of human serum albumin (1mg/ml). Hence, HSA was incubated 30 min with various combinations of a Fenton system and quantified oxidation products such as carbonyl groups, fragmentations, degradations, and oxidized free thiol group using reliable techniques. Image and data analysis were carried out using ImageJ software and Excel (version 2007), respectively.

Results: An SDS-PAGE profile showed no cross link and aggregation. However, protein band intensity has decreased to 50% in the highest ratio of HO/Fe. Carbonylation assay indicated carbonyl/protein (molc/molp) ratio increased linearly in lower ratios and the values plateau at higher levels of HO/Fe 2+. The only free sulfhydryl group on HSA was oxidized in all ratios of the Fenton system.

Conclusion: To sum, the structure of HSA has been changed following treatment with Hydroxyl Radical as the main product of Fenton reaction. These data confirm the antioxidant activity of HSA.
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http://dx.doi.org/10.19082/2970DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5074758PMC
September 2016