Publications by authors named "Du Xiang"

173 Publications

Artificially created interfacial states enabled van der Waals heterostructure memory device.

Nanotechnology 2022 Jan 13. Epub 2022 Jan 13.

Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 117543, SINGAPORE.

Two-dimensional (2D) interface plays a predominate role in determining the performance of a device that is configured as a van der Waals heterostructure (vdWH). Intensive efforts have been devoted to suppressing the emergence of interfacial states during vdWH stacking process, which facilitates the charge interaction and transfer between the heterostructure layers. However, the effective generation and modulation of the vdWH interfacial states could give rise to a new design and architecture of 2D functional devices. Here, we report a 2D non-volatile vdWH memory device enabled by the artificially created interfacial states between hexagonal boron nitride (hBN) and molybdenum ditelluride (MoTe2). The memory originates from the microscopically coupled optical and electrical responses of the vdWH, with the high reliability reflected by its long data retention time over 10^4 s and large write-erase cyclic number exceeding 100. Moreover, the storage currents in the memory can be precisely controlled by the writing and erasing gates, demonstrating the tunability of its storage states. The vdWH memory also exhibits excellent robustness with wide temperature endurance window from 100 K to 380 K, illustrating its potential application in harsh environment. Our findings promise interfacial-states engineering as a powerful approach to realize high performance vdWH memory device, which opens up new opportunities for its application in 2D electronics and optoelectronics.
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http://dx.doi.org/10.1088/1361-6528/ac4b2fDOI Listing
January 2022

Friend or Foe? The Roles of Antioxidants in Acute Lung Injury.

Antioxidants (Basel) 2021 Dec 7;10(12). Epub 2021 Dec 7.

Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, Wuhan 430071, China.

Acute lung injury (ALI) is an acute hypoxic respiratory insufficiency caused by various intra- and extra-pulmonary injury factors. The oxidative stress caused by excessive reactive oxygen species (ROS) produced in the lungs plays an important role in the pathogenesis of ALI. ROS is a "double-edged sword", which is widely involved in signal transduction and the life process of cells at a physiological concentration. However, excessive ROS can cause mitochondrial oxidative stress, leading to the occurrence of various diseases. It is well-known that antioxidants can alleviate ALI by scavenging ROS. Nevertheless, more and more studies found that antioxidants have no significant effect on severe organ injury, and may even aggravate organ injury and reduce the survival rate of patients. Our study introduces the application of antioxidants in ALI, and explore the mechanisms of antioxidants failure in various diseases including it.
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http://dx.doi.org/10.3390/antiox10121956DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8750496PMC
December 2021

Ionizing Radiation-Induced RPL23a Reduction Regulates Apoptosis RPL11-MDM2-p53 Pathway in Mouse Spermatogonia.

Biomed Environ Sci 2021 Oct;34(10):789-802

College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, Gansu, China.

Objective: The expression patterns of ribosomal large subunit protein 23a (RPL23a) in mouse testes and GC-1 cells were analyzed to investigate the potential relationship between RPL23a expression and spermatogonia apoptosis upon exposure to X-ray.

Methods: Male mice and GC-1 cells were irradiated with X-ray, terminal dUTP nick end-labelling (TUNEL) was performed to detect apoptotic spermatogonia . Apoptotic rate and cell cycle phase of GC-1 cells were analyzed with flow cytometry. Protein interactions were detected by Immunoprecipitation and protein localization as studied by immunofluorescence. Immunoblotting and real-time PCR were applied to analyze to protein and gene expression.

Results: Ionizing radiation (IR) increased spermatogonia apoptosis, the expression of RPL11, MDM2 and p53, and decreased RPL23a expression in mice spermatogonia and . RPL23a knockdown weakened the interaction between RPL23a and RPL11, leading to p53 accumulation. Moreover, knockdown and IR decreased RPL23a that induces spermatogonia apoptosis RPL23a-RPL11-MDM2-p53 pathway in GC-1 cells.

Conclusion: These results suggested that IR reduced RPL23a expression, leading to weakened the RPL23a-RPL11 interactions, which may have activated p53, resulting in spermatogonia apoptosis. These results provide insights into environmental and clinical risks of radiotherapy following exposure to IR in male fertility. The graphical abstract was available in the web of www.besjournal.com.
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http://dx.doi.org/10.3967/bes2021.108DOI Listing
October 2021

Erratum to "The Inhibition of P-Selectin Reduced Severe Acute Lung Injury in Immunocompromised Mice".

Oxid Med Cell Longev 2021 28;2021:9827438. Epub 2021 Sep 28.

Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University; Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation, Wuhan 430071, China.

[This corrects the article DOI: 10.1155/2020/8430465.].
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http://dx.doi.org/10.1155/2021/9827438DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8492243PMC
September 2021

[Effect on the core symptoms in children with autism spectrum disorder treated with acupuncture at "ghost points" combined with the acupoints selected by syndrome differentiation].

Zhen Ci Yan Jiu 2021 Aug;46(8):695-9

Children Encephalopathy Rehabilitation Center of the Third People's Hospital of Hubei Province Affiliated to Jianghan University, Wuhan 430033, China.

Objective: To explore the effect on the core symptoms in children with autism spectrum disorder (ASD) treated with acupuncture at "ghost points", combined with the acupoints selected by syndrome differentiation.

Methods: A total of 124 ASD children were divided into a control group and a treatment group according to random number table, 62 cases in each group. In the control group, the routine rehabilitation treatment was provided in all of children, while in the treatment group, acupuncture was exerted at "ghost points", combined with the acupoints selected by syndrome differentiation. Acupuncture treatment was conducted for 15 min each time, 5 days weekly, for 3 months totally. Before and after treatment, the children were assessed with autism spectrum rating scale (ASRS) in two groups.

Results: After treatment, the total score (T-score) of ASRS and the score of each sub-scale, e.g. peer socialization, adult socialization, social/emotional reciprocity, atypical language, stereotypy, repetitive behavior, sensory sensitivity, attention/self-regulation were all reduced remarkably as compared with those before treatment in intra-group comparison (<0.05, <0.01). Compared with the control group, the scores aforementioned were all significantly reduced in the treatment group (<0.05).

Conclusion: Acupuncture at "ghost poitns" combined with the acupoints selected by syndrome differentiation effectively relieves the core symptoms of ASD in children and improves the holistic therapeutic effect.
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http://dx.doi.org/10.13702/j.1000-0607.200652DOI Listing
August 2021

[A method of marking neurons on brain slices using patch clamp technique].

Zhongguo Ying Yong Sheng Li Xue Za Zhi 2021 Jul;37(4):445-448

Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, Taiyuan 030001, China.

To introduce a method of marking neurons using patch clamp technique. The brain slices of the target area was cut with a vibrating microtome. The glass microelectrode was perfused with the electrode liquid containing Neurobiotin Tracer, and the whole-cell patch-clamp recording was performed. After recording, the brain slices were fixed and rinsed with 4% paraformaldehyde. After stained in phosphate buffer with Streptavidin-Texas Red and Triton X-100 for at least 2 hours, the neurons can be observed under a fluorescence microscope. The cell membrane voltage was clamped at -70 mV, and the neuron showed a gradually increasing membrane current after step stimulation. When recording in the current clamp mode, the step stimulus caused the neuron to depolarize to the threshold potential and then burst into action potentials. The morphology of intact neurons with clear cell body and protrusions of a neuron could be observed under a fluorescence microscope. This method is suitable for observing the morphological features of the recorded neuron after patch clamp experiments, which is easy to operate, and the image is intuitive and clear.
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http://dx.doi.org/10.12047/j.cjap.6035.2021.031DOI Listing
July 2021

Protective Effects of Estrogen on Cardiovascular Disease Mediated by Oxidative Stress.

Oxid Med Cell Longev 2021 28;2021:5523516. Epub 2021 Jun 28.

Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, Wuhan 430071, China.

Perimenopause is an important stage of female senescence. Epidemiological investigation has shown that the incidence of cardiovascular disease in premenopausal women is lower than that in men, and the incidence of cardiovascular disease in postmenopausal women is significantly higher than that in men. This phenomenon reveals that estrogen has a definite protective effect on the cardiovascular system. In the cardiovascular system, oxidative stress is considered important in the pathogenesis of atherosclerosis, myocardial dysfunction, cardiac hypertrophy, heart failure, and myocardial ischemia. From the perspective of oxidative stress, estrogen plays a regulatory role in the cardiovascular system through the estrogen receptor, providing strategies for the treatment of menopausal women with cardiovascular diseases.
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http://dx.doi.org/10.1155/2021/5523516DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260319PMC
January 2022

RNA-binding protein IMP3 is a novel regulator of MEK1/ERK signaling pathway in the progression of colorectal Cancer through the stabilization of MEKK1 mRNA.

J Exp Clin Cancer Res 2021 Jun 21;40(1):200. Epub 2021 Jun 21.

Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China.

Background: MEK1/ERK signaling pathway plays an important role in most tumor progression, including colorectal cancer (CRC), however, MEK1-targeting therapy has little effective in treating CRC patients, indicating there may be a complex mechanism to activate MEK1/ERK signaling pathway except RAS activated mechanism.

Methods: To investigate the clinical significance of IMP3, we analyzed its expression levels in publicly available dataset and samples from Fudan University Shanghai Cancer Center. The effects of IMP3 on proliferation, migration, and invasion were determined by in vitro and in vivo experiments. To investigate the role of IMP3 in colon carcinogenesis, conditional IMP3 knockout C57BL/6 mice was generated. The IMP3/MEKK1/MEK/ERK signaling axis in CRC was screened and validated by RNA-sequencing, RNA immunoprecipitation, luciferase reporter and western blot assays.

Results: We find RNA binding protein IMP3 directly bind to MEKK1 mRNA 3'-UTR, which regulates its stability, promote MEKK1 expression and sequentially activates MEK1/ERK signaling. Functionally, IMP3 promote the malignant biological process of CRC cells via MEKK1/MEK1/ERK signaling pathway both in vitro and in vivo, Moreover, IMP3 mice show decreased the expression of MEKK1 as well as colorectal tumors compared with wild-type mice after treatment with azoxymethane/dextran sodium sulfate. Clinically, the expression of IMP3 and MEKK1 are positive correlated, and concomitant IMP3 and MEKK1 protein levels negatively correlate with metastasis in CRC patients. In addition, MEK1 inhibitor in combination with shRNA-IMP3 have a synergistic effect both in vitro and in vivo.

Conclusion: Our study demonstrates that IMP3 regulates MEKK1 in CRC, thus activating the MEK1/ERK signaling in the progression of colorectal cancer, Furthermore, these results provide new insights into potential applications for combining MEK1 inhibitors with other target therapy such as IMP3 in preclinical trials for CRC patients.
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http://dx.doi.org/10.1186/s13046-021-01994-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8215736PMC
June 2021

Advances in the applications of polymer biomaterials for in vitro follicle culture.

Biomed Pharmacother 2021 Aug 5;140:111422. Epub 2021 Jun 5.

Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation, 169 Donghu Road, Wuhan, Hubei 430071, China. Electronic address:

The ovarian reserve (OR) indicates ovarian function by representing the quantity and quality of ovarian follicles, and it gradually decreases with increasing age. With the prolongation of women's lives, the protection provided by estrogen is lost for decades in postmenopausal women, and the related cardiovascular and cerebrovascular diseases, osteoporosis, and decreased immunity are the main risk factors affecting women's quality of life and longevity. Pharmacologic hormone replacement therapy (PHRT) has been controversial, and the construction of artificial ovary (AO) has attracted increasing attention. The most critical step of AO generation is the establishment of an in vitro culture (IVC) system to support the development of isolated follicles. This article mainly compares the advantages and disadvantages of different polymer biomaterials for use in follicle IVC, provides theoretical support for the development and construction of the follicle IVC system using natural biological materials, and provides a theoretical basis for establishing mature AO technology.
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http://dx.doi.org/10.1016/j.biopha.2021.111422DOI Listing
August 2021

Dielectric Engineered Two-Dimensional Neuromorphic Transistors.

Nano Lett 2021 04 9;21(8):3557-3565. Epub 2021 Apr 9.

Department of Chemistry, National University of Singapore, Singapore 117543, Singapore.

Two-dimensional (2D) materials, which exhibit planar-wafer technique compatibility and pure electrically triggered communication, have established themselves as potential candidates in neuromorphic architecture integration. However, the current 2D artificial synapses are mainly realized at a single-device level, where the development of 2D scalable synaptic arrays with complementary metal-oxide-semiconductor compatibility remains challenging. Here, we report a 2D transition metal dichalcogenide-based synaptic array fabricated on commercial silicon-rich silicon nitride (sr-SiN) substrate. The array demonstrates uniform performance with sufficiently high analogue on/off ratio and linear conductance update, and low cycle-to-cycle variability (1.5%) and device-to-device variability (5.3%), which are essential for neuromorphic hardware implementation. On the basis of the experimental data, we further prove that the artificial synapses can achieve a recognition accuracy of 91% on the MNIST handwritten data set. Our findings offer a simple approach to achieve 2D synaptic arrays by using an industry-compatible sr-SiN dielectric, promoting a brand-new paradigm of 2D materials in neuromorphic computing.
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http://dx.doi.org/10.1021/acs.nanolett.1c00492DOI Listing
April 2021

Insights into measurements of water-soluble ions in PM and their gaseous precursors in Beijing.

J Environ Sci (China) 2021 Apr 1;102:123-137. Epub 2020 Oct 1.

Institute of Urban Meteorology, China Meteorological Administration, Beijing 100089, China.

To better understand the characteristics and transformation mechanisms of secondary inorganic aerosols, hourly mass concentrations of water-soluble inorganic ions (WSIIs) in PM and their gaseous precursors were measured online from 2016 to 2018 at an urban site in Beijing. Seasonal and diurnal variations in water-soluble ions and gaseous precursors were discussed and their gas-particle conversion and partitioning were also examined, some related parameters were characterized. The (TNH) was also defined to describe the variations of the excess NH in different seasons. In addition, a sensitivity test was carried out by using ISORROPIA II to outline the driving factors of gas-particle partitioning. In Beijing, the relative contribution of nitrate to PM has increased markedly in recent years, especially under polluted conditions. In the four seasons, only a small portion of NO in the atmosphere was converted into total nitrate (TNO) and more than 80% of TNO occurred in the form of nitrate due to the abundant ammonia. The concentration of total ammonia (TNH) was much higher than that required to neutralize acid gases, and most of the TNH occurred as gaseous NH. The nitrous acid (HONO) concentration was highly correlated with NH concentration and had increased significantly in Beijing compared with previous studies. The total chloride (TCl) was the highest in winter, and ε(Cl) was more sensitive to variations in the ambient temperature (T) and relative humidity (RH) than ε(NO).
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http://dx.doi.org/10.1016/j.jes.2020.08.031DOI Listing
April 2021

Broadband Spin-Dependent Directional Coupler via Single Optimized Metallic Catenary Antenna.

Materials (Basel) 2021 Jan 10;14(2). Epub 2021 Jan 10.

College of Physics and Electronic Technology, Anhui Normal University, Wuhu 241000, China.

With the rapid development of on-chip optics, integrated optical devices with better performance are desirable. Waveguide couplers are the typical integrated optical devices, allowing for the fast transmission and conversion of optical signals in a broad working band. However, traditional waveguide couplers are limited by the narrow operation band to couple the spatial light into the chip and the fixed unidirectional transmission of light flow. Furthermore, most of the couplers only realize unidirectional transmission under the illumination of the linear polarized light. In this work, a broadband polarization directional coupler based on a metallic catenary antenna integrated on a silicon-on-insulator (SOI) waveguide has been designed and demonstrated under the illumination of the circularly polarized light. By applying the genetic algorithm to optimize the multiple widths of the metallic catenary antenna, the numerical simulation results show that the extinction ratio of the coupler can be maintained larger than 18 dB in a wide operation band of 300 nm (from 1400 to 1700 nm). Moreover, the coupler can couple the spatial beam into the plane and transmit in the opposite direction by modulating the rotation direction of the incident light. The broadband polarization directional coupler might have great potential in integrated optoelectronic devices and on-chip optical devices.
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http://dx.doi.org/10.3390/ma14020326DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827409PMC
January 2021

Hyoid bone position in subjects with different facial growth patterns of different dental ages.

Cranio 2021 Jan 11:1-7. Epub 2021 Jan 11.

Department of Orthodontics, Hospital of Stomatology, Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, Xi'an Jiaotong University, Xi'an, China.

: To explore the correlation between hyoid bone (HB) positions and facial growth patterns (facial patterns) in Chinese adults; to identify any significant difference in HB position among subjects with different facial patterns in various dental ages.: Lateral cephalometric radiographs of 197 Chinese subjects were divided into nine groups based on their dental ages and facial patterns. Seven measurements were used to define HB position. Regression, correlation analyses, and one-way ANOVA were carried out.: Significant correlations were found between facial patterns and anteroposterior HB positions. The HB was more anterior in the horizontal group after mixed dentition and further away from the mandibular plane in the vertical group of adults. Vertical HB positions were insignificantly different in any stage.: HB position and facial patterns were correlated. There were significantly different HB positions among people with different facial patterns in various dental ages.
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http://dx.doi.org/10.1080/08869634.2020.1867471DOI Listing
January 2021

A standardized pathological proposal for evaluating microvascular invasion of hepatocellular carcinoma: a multicenter study by LCPGC.

Hepatol Int 2020 Dec 28;14(6):1034-1047. Epub 2020 Dec 28.

Department of Pathology, The First Affiliated Hospital of Dalian Medical University, Dalian, China.

Background And Aims: Microvascular invasion (MVI) is a key pathological factor that severely affects the postoperative prognosis of patients with hepatocellular carcinoma (HCC). However, no MVI classification schemes based on standardized gross sampling protocols of HCC are available at present.

Methods: 119 HCC specimens were sampled at multiple sites (3-, 7-, and 13 points) for the optimum MVI detection rate. 16,144 resected HCCs were graded as M0, M1 or M2 by adopting three-tiered MVI grading (MVI-TTG) scheme based on the seven-point sampling protocol (SPSP). Survival analyses were performed on 2573 patients to explore the advantages of MVI-TTG.

Results: The MVI detection rate determined by SPSP was significantly higher than that determined by the 3-point sampling method (34.5% vs. 47.1%, p = 0.048), but was similar to that determined by the 13-point sampling method (47.1% vs. 51.3%, p = 0.517). Among 16,144 resected HCCs, the proportions of M0, M1 and M2 specimens according to SPSP were 53.4%, 26.2% and 20.4%, respectively. Postoperative survival analysis in 2573 HCC patients showed that the 3-year recurrence rates in M0, M1 and M2 MVI groups were 62.5%, 71.6% and 86.1%, respectively (p < 0.001), and the corresponding 3-year overall survival (OS) rates were 94.1%, 87.5% and 67.0%, respectively (p < 0.001). M1 grade was associated with early recurrence, while M2 grade was associated with both early and late recurrence. MVI-TTG had a larger area under the curve and net benefit rate than the two-tiered MVI grading scheme for predicting time to recurrence and OS.

Conclusions: SPSP is a practical method to balance the efficacy of sampling numbers and MVI detection rates. MVI-TTG based on SPSP is a better prognostic predictor than the two-tiered MVI scheme. The combined use of SPSP and MVI-TTG is recommended for the routine pathological diagnosis of HCC.
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http://dx.doi.org/10.1007/s12072-020-10111-4DOI Listing
December 2020

Hypoxia-Inducible Factor-1: A Potential Target to Treat Acute Lung Injury.

Oxid Med Cell Longev 2020 17;2020:8871476. Epub 2020 Nov 17.

Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, Wuhan 430071, China.

Acute lung injury (ALI) is an acute hypoxic respiratory insufficiency caused by various intra- and extrapulmonary injury factors. Presently, excessive inflammation in the lung and the apoptosis of alveolar epithelial cells are considered to be the key factors in the pathogenesis of ALI. Hypoxia-inducible factor-1 (HIF-1) is an oxygen-dependent conversion activator that is closely related to the activity of reactive oxygen species (ROS). HIF-1 has been shown to play an important role in ALI and can be used as a potential therapeutic target for ALI. This manuscript will introduce the progress of HIF-1 in ALI and explore the feasibility of applying inhibitors of HIF-1 to ALI, which brings hope for the treatment of ALI.
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http://dx.doi.org/10.1155/2020/8871476DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7685819PMC
June 2021

Optically Controllable 2D Material/Complex Oxide Heterointerface.

Adv Sci (Weinh) 2020 Nov 20;7(21):2002393. Epub 2020 Aug 20.

Department of Chemistry National University of Singapore 3 Science Drive 3 Singapore 117543 Singapore.

Heterostructures play a vital role in functional devices on the basis of the individual constituents. Non-conventional heterostructures formed by stacking 2D materials onto structurally distinct materials are of great interest in achieving novel phenomena that are both scientifically and technologically relevant. Here, a heterostructure based on a 2D (molybdenum ditelluride) MoTe and an amorphous strontium titanium oxide (a-STO) thin film is reported. The heterostructure functions as a high-performance photodetector, which exhibits anomalous negative photoresponse in the pristine device due to the scattering effect from the light-induced O ions. The photoresponsivity and the specific detectivity are found to be >10 AW and >10 Jones, respectively, which are significantly higher than those in standard MoTe devices. Moreover, through tuning the light programming time, the photodetection behavior of the MoTe/a-STO heterostructure experiences a dynamic evolution from negative to positive. This is due to the optically controllable modulation of the interfacial states, which is further confirmed by the X-ray photoelectron spectroscopy and photoluminescence measurements. It is envisioned that the 2D material/a-STO heterostructure could be a potential platform for exploring new functional devices.
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http://dx.doi.org/10.1002/advs.202002393DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7610330PMC
November 2020

GCNT4 is Associated with Prognosis and Suppress Cell Proliferation in Gastric Cancer.

Onco Targets Ther 2020 25;13:8601-8613. Epub 2020 Aug 25.

Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai 200032, People's Republic of China.

Background: GCNT4 is a member of the glucosaminyl (N-acetyl) transferases family that has been implicated in multiple human malignancies. However, the role of GCNT4 in gastric cancer (GC) is unknown. In this present study, we aimed to explore the role and clinicopathological correlation of GCNT4 in GC.

Materials And Methods: We first evaluated the dysregulation of GCNT4 in The Cancer Genome Atlas (TCGA) and then we performed RT-qPCR and immunohistochemistry to validate the results in a cohort of in-house patients. The clinicopathological correlation and function of GCNT4 in GC were also analysed.

Results: GCNT4 was found to be significantly downregulated in GC. In addition, GCNT4 expression correlated with tumour depth, nervous invasion and pathological tumor-node-metastasis (pTNM) stage. Moreover, lower GCNT4 levels conferred poor overall survival (OS) and disease-free survival (DFS) to GC patients. Multivariate Cox regression analysis revealed that GCNT4 protein expression is an independent prognostic factor for OS in patients with GC. Further functional experimental results revealed that overexpression of GCNT4 appears to halt GC cell proliferation and the cell cycle.

Conclusion: Altogether, these findings indicated that GCNT4 regulates the GC cell cycle and have important implications for the selection of therapeutic targets to prevent tumour proliferation.
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http://dx.doi.org/10.2147/OTT.S248997DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7457769PMC
August 2020

Re-positive Cases of Nucleic Acid Tests in Discharged Patients With COVID-19: A Follow-Up Study.

Front Med (Lausanne) 2020 23;7:349. Epub 2020 Jun 23.

Center for Primary Health Care Research, Lund University/Region Skåne, Skåne University Hospital, Malmö, Sweden.

The frequent emergence of the re-positive patients with COVID-19 is a potential threat worldwide. This study aimed to describe data from admission to follow-up for patients with COVID-19 and analyze the possible causes for re-positive nucleic acid tests to provide more scientific basis for reducing the numbers of re-positive patients after discharge. We retrospectively recorded 15 patients with COVID-19 admitted to the Xianyang Central Hospital, China. The baseline, exposure histories, clinical syndromes, laboratory characteristics, nucleic acid, and follow-up tests were analyzed, and the radiological characteristics of re-positive patient at different periods were compared. Eight (53.33%) patients had the history of travel to Wuhan, four (26.67%) patients had close contact with confirmed patients, and one (6.67%) patient had close contact with suspected patients. After treatment, all patients had two consecutively negative nucleic acid tests and were discharged from hospital. All patients were followed up for more than 14 days, and the average time from discharge to the first follow-up was 14.67 ± 3.31 days (from 9 to 22 days). Most patients showed no clinical symptoms and negative nucleic acid tests, while one patient had an itchy throat, her CT scan showed a light density shadow in the right lower lobe of the lung, and the nucleic acid was once again positive. The second follow-up of the other 14 patients (except the re-positive one) was conducted 20.80 ± 7.78 days (from 13 to 30 days) after discharge, and all of them had negative nucleic acid tests. The positive patient was immediately readmitted and received a new round of treatment. Her family members and colleagues remained healthy until now. The quality of nucleic acid testing reagents should be enhanced, and the training of nucleic acid sampling operators should be strengthened to reduce the false-negative results in the nucleic acid of SARS-CoV-2; the clinical specimens of throat and nasopharynx swabs can be collected at the same time; IgM- and IgG-specific antibodies of SARS-CoV-2 should be carried out for discharged patients; the radiological characteristics should be evaluated strictly; and the discharge standard can be specified according to the baseline and severity of disease of patients.
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http://dx.doi.org/10.3389/fmed.2020.00349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324684PMC
June 2020

Development and Clinical Validation of a 90-Gene Expression Assay for Identifying Tumor Tissue Origin.

J Mol Diagn 2020 09 28;22(9):1139-1150. Epub 2020 Jun 28.

Cancer of Unknown Primary Group, Pathology Committee, Chinese Research Hospital Association, Shanghai, People's Republic of China; Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, People's Republic of China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, People's Republic of China. Electronic address:

The accurate identification of tissue origin in patients with metastatic cancer is critical for effective treatment selection but remains a challenge. The aim of this study is to develop a gene expression assay for tumor molecular classification and integrate it with clinicopathologic evaluations to identify the tissue origin for cancer of uncertain primary (CUP). A 90-gene expression signature, covering 21 tumor types, was identified and validated with an overall accuracy of 89.8% (95% CI, 0.87-0.92) in 609 tumor samples. More specifically, the classification accuracy reached 90.4% (95% CI, 0.87-0.93) for 323 primary tumors and 89.2% (95% CI, 0.85-0.92) for 286 metastatic tumors, with no statistically significant difference (P = 0.71). Furthermore, in a real-life cohort of 141 CUP patients, predictions by the 90-gene expression signature were consistent or compatible with the clinicopathologic features in 71.6% of patients (101/141). Findings suggest that this novel gene expression assay could efficiently predict the primary origin for a broad spectrum of tumor types and support its diagnostic utility of molecular classification in difficult-to-diagnose metastatic cancer. Additional studies are ongoing to further evaluate the clinical utility of this novel gene expression assay in predicting primary site and directing therapy for CUP patients.
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http://dx.doi.org/10.1016/j.jmoldx.2020.06.005DOI Listing
September 2020

Polarized Light-Emitting Diodes Based on Anisotropic Excitons in Few-Layer ReS.

Adv Mater 2020 Aug 30;32(32):e2001890. Epub 2020 Jun 30.

Department of Physics, National University of Singapore, 2 Science Drive 3, Singapore, 117551, Singapore.

An on-chip polarized light source is desirable in signal processing, optical communication, and display applications. Layered semiconductors with reduced in-plane symmetry have inherent anisotropic excitons that are attractive candidates as polarized dipole emitters. Herein, the demonstration of polarized light-emitting diode based on anisotropic excitons in few-layer ReS , a 2D semiconductor with excitonic transition energy of 1.5-1.6 eV, is reported. The light-emitting device is based on minority carrier (hole) injection into n-type ReS through a hexagonal boron nitride (hBN) tunnel barrier in a metal-insulator-semiconductor (MIS) van der Waals heterostack. Two distinct emission peaks from excitons are observed at near-infrared wavelength regime from few-layer ReS . The emissions exhibit a degree of polarization of 80% reflecting the nearly 1D nature of excitons in ReS .
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http://dx.doi.org/10.1002/adma.202001890DOI Listing
August 2020

THE FREQUENCIES OF X-RAY EXAMINATIONS AND CT SCANS: FINDINGS FROM A SAMPLE INVESTIGATION IN JIANGSU, CHINA.

Radiat Prot Dosimetry 2020 Aug;190(1):38-44

Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu, China.

Objective: The present study was performed to evaluate the frequencies of two kinds of examinations using a proportional sample of 262 medical institutions and to observe the factors affecting the amount of examination-related exposure. The other aim of the present study was to observe the relationship between X-ray and CT frequency with GDP per capita, which could indicate the connection between medical exposure practice and economy.

Methods: A random sample was taken from a pool of 316 medical institutions, and correlation analyses were performed to identify the factors affecting the amount of examination-related exposure. A representative sample of 262 medical institutions, proportional to the distribution of hospitals across grades, was used, and a multiple linear regression model was constructed.

Results: The frequencies of X-ray examinations and CT scans were 523 per 1000 people and 223 per 1000 people, respectively. The two kinds of radio-diagnostic examinations showed different patterns in their relationships with GDP per capita. The factors correlated with the amounts of exposure due to the two kinds of examinations and the outpatient and equipment numbers showed distinctive patterns in the group of grade three institutions.

Conclusions: The improvement in the economy has caused a rapid increase in the use of radio-diagnostic examinations. The differences in factors correlated with the two types of examinations may stem from the workload statuses of CT scans and X-ray examinations in grade three hospitals.
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http://dx.doi.org/10.1093/rpd/ncaa076DOI Listing
August 2020

Elevated activity of plasma superoxide dismutase in never-treated first-episode schizophrenia patients: Associated with depressive symptoms.

Schizophr Res 2020 08 22;222:291-296. Epub 2020 May 22.

CAS Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing, China; Department of Psychology, University of Chinese Academy of Sciences, Beijing, China. Electronic address:

Oxidative stress in excess may be engaged in the pathophysiological development of schizophrenia (SCZ). Previous research showed altered activity of superoxide dismutase (SOD) in patients suffering from SCZ, with inconsistent results. However, few studies have analyzed the relationship between SOD activity and psychopathological symptoms in never-treated first-episode (NTFE) patients with SCZ. The activities of manganese SOD (MnSOD) and total SOD were measured in a large sample of 166 NTFE patients with SCZ, and 133 healthy controls. The patients' symptoms were evaluated by the Positive and Negative Syndrome Scale (PANSS), as well as the depressive and cognitive factors originated from the PANSS five-factor model. NTFE patients had significantly higher activities of MnSOD and total SOD than healthy controls (both p < 0.01). Correlation analysis displayed a notably positive correlation between both MnSOD or total SOD activities and the PANSS depressive factor, as well as between MnSOD activity and the PANSS general psychopathology subscale score (all p < 0.05). Stepwise multiple regression analysis revealed that both MnSOD and total SOD were independent factors affecting PANSS depressive factor and PANSS general psychopathology subscale score. Our findings suggest that increased SOD activity may be associated with comorbid depressive symptoms in NTFE patients with SCZ.
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http://dx.doi.org/10.1016/j.schres.2020.05.032DOI Listing
August 2020

Hypothermic machine perfusion after static cold storage improves ovarian function in rat ovarian tissue transplantation.

J Assist Reprod Genet 2020 Jul 20;37(7):1745-1753. Epub 2020 May 20.

Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation, Wuhan, Hubei, 430071, China.

Objective: This study was performed to investigate the effect of hypothermic machine perfusion (HMP) after cold storage (CS) on ovarian transplantation.

Methods: Rats aged 8-10 weeks were used as the donors and recipients for allotransplantation. Eighteen donor rats were divided into three groups: the fresh control (n = 6), cold storage (CS; n = 6), and hypothermic machine perfusion (HMP; n = 6) groups. The preservation solution contained Dulbecco's modified Eagle's medium/Ham's F-12 (1:1, v/v), 10% fetal bovine serum, 10 μg/ml insulin, 10 μg/ml transferrin, and 50 mIU/ml follicle-stimulating hormone (FSH). The donor ovaries in the CS and HMP groups were excised and then respectively subjected to 4 h of CS and 2 h of CS combined with 2 h of HMP at 4 °C, and then transplanted beneath the recipient's left renal capsule. At 7 days after transplantation, the ovaries were removed and blood samples were obtained for histological analysis, immunohistochemistry for CD31 and Ki67, and serum anti-Mullerian hormone (AMH) level estimation.

Results: The HMP group showed significant increases in serum AMH and CD31-positive areas when compared to these values in the CS group (P < 0.05). However, no differences were noted in the total number of follicles or the Ki67-positive areas among the three groups.

Conclusion: Hypothermic machine perfusion after static cold storage is more effective than static CS alone for the short-term preservation of whole ovaries during transport. Whole ovary transplantation with vascular pedicle is our future research direction. Graphical Abstract The black rectangle in the figure shows the place where ligation and disconnection are required, the black dotted line shows the place where vascular forceps are used to clamp, and the black circle shows the place where the cannula is inserted This diagram was made for reviewers to understand more intuitively how my hypothermia mechanical perfusion model was built. Organs obtained in this way can be used for subsequent perfusion and whole ovarian transplantation.
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http://dx.doi.org/10.1007/s10815-020-01797-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7376786PMC
July 2020

The Inhibition of P-Selectin Reduced Severe Acute Lung Injury in Immunocompromised Mice.

Oxid Med Cell Longev 2020 23;2020:8430465. Epub 2020 Apr 23.

Zhongnan Hospital of Wuhan University; Institute of Hepatobiliary Diseases of Wuhan University; Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation, Wuhan 430071, China.

In an immunocompetent host, excess infiltration of immune cells in the lung is a key factor in infection-induced severe acute lung injury. Kidney transplant patients are immunocompromised by the use of immunosuppressive drugs. Immune cell infiltration in the lung in a renal transplant recipient suffering from pulmonary infection is significantly less than that in an immunocompetent host; however, the extent of lung injury in renal transplant patients is more serious than that in immunocompetent hosts. Therefore, we explored the role of platelet activation in a Klebsiella pneumoniae-induced lung injury model with P-selectin gene knockout mice or wild-type mice. Our study suggested that the inhibition of platelets reduced severe acute lung injury and increased survival after acute lung infection in mice. In addition, P-selectin expression on the surface of platelets in mice increased after administration of immunosuppressive drugs, and the extent of lung injury induced by infection decreased in P-selectin gene knockout mice. In conclusion, p-selectin plays a key role in severe acute lung injury in immunocompromised mice by reducing platelet activation and inflammatory processes.
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http://dx.doi.org/10.1155/2020/8430465DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7196163PMC
February 2021

Native Oxide Seeded Spontaneous Integration of Dielectrics on Exfoliated Black Phosphorus.

ACS Appl Mater Interfaces 2020 May 12;12(21):24411-24418. Epub 2020 May 12.

Department of Physics, National University of Singapore, Singapore 117543, Singapore.

Two-dimensional (2D) semiconductors have been a central focus for next-generation electronics and optoelectronics owing to their great potential to extend the scaling limits in a silicon transistor. However, due to the lack of surface dangling bonds in most 2D semiconductors, such as graphene and transition metal dichalcogenides (TMDs), the direct growth of the high-κ film on these 2D materials via an atomic layer deposition (ALD) technique often produces dielectrics with poor quality, which hinders their integration in the modern semiconductor industry. Here, we comprehensively investigate the ALD growth of the AlO layer on 2D exfoliated black phosphorus (BP). Intriguingly, we found that the 2D BP with "silicon-like" characteristics possesses a native surface oxide layer PO after air exposure. The PO-induced surface dangling bonds enable the spontaneous integration of the high-quality AlO layer on the BP flake without any pretreatments to functionalize the surface. Additionally, the AlO layer could effectively passivate BP to prevent its degradation in ambient conditions, which addresses the most serious problem of the BP material. Moreover, the AlO-encapsulated BP field-effect transistor (FET) exhibits good electrical transport performance, with a high hole mobility of ∼420 cm V s and electron mobility of ∼80 cm V s. Moreover, the high-quality AlO layer can also be integrated into the top-gated BP transistor and inverter. Our findings reveal the silicon-like characteristics of BP for the high-κ ALD dielectric growth technology, which promises the seamless integration of 2D BP in the modern semiconductor industry.
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http://dx.doi.org/10.1021/acsami.0c01161DOI Listing
May 2020

Aerosol hygroscopicity based on size-resolved chemical compositions in Beijing.

Sci Total Environ 2020 May 1;716:137074. Epub 2020 Feb 1.

Institute of Urban Meteorology, China Meteorology Administration, Beijing 100089, China.

Hygroscopicity is an important property of aerosols, which cannot be obtained for a wide range of particle sizes by online observation. A cascade impactor sampler, an essential method for obtaining the size-resolved chemical compositions of ambient aerosols, enables the acquisition of the size-resolved hygroscopicity for particles in multiple modes. A micro-orifice uniform deposit impactor (MOUDI-122, MSP) was used to collect the size-resolved aerosol samples during 2016 and 2017. The water-soluble components in these samples were analysed for different pollution levels in two periods. Then, the hygroscopicity parameter (κ) was calculated. The changing hygroscopicity in different size ranges was directly influenced by variations in the distribution of the water-soluble components. The contribution of sulfate to the κ was much higher in the summer period than that in the winter period due to the higher SO concentration in the summer. During the summer period, the contribution of nitrate to the κ of particle sizes above 0.56 μm was significantly higher than that of particles smaller than 0.56 μm, while in the winter period, the contribution of nitrate in finer particles with sizes below 1.8 μm was much higher than that in coarse particles. The contribution of chloride to the κ increased significantly in the winter period due to the influence of heating coal emissions. For particles below 1.0 μm, the contribution and fraction of water-soluble organic compounds (WSOCs) to the aerosol hygroscopicity increased with decreasing particle size. Compared with the aerosol hygroscopicity during the first stage of a pollution episode, the hygroscopicity of particles above 0.18 μm was significantly enhanced during the stages of pollution accumulation and formation. The results in this study were in good agreement with the results of other similar studies or data derived by other methods, indicating that the hygroscopicity based on size-resolved water-soluble components is reliable and can be used in the study of activation, radiation force, and heterogeneous reaction mechanism of particles with multiple sizes.
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http://dx.doi.org/10.1016/j.scitotenv.2020.137074DOI Listing
May 2020

[Size Distributions of Different Carbonaceous Components in Ambient Aerosols].

Huan Jing Ke Xue 2019 Sep;40(9):3849-3855

Institute of Urban Meteorology, China Meteorological Administration, Beijing 100089, China.

It is important to obtain the size distribution of carbonaceous components in aerosols for studying the formation and transformation mechanisms and radiation characteristics of regional aerosols. However, only a few studies on the size distribution of aerosol carbonaceous fractions have been conducted in Beijing. In this study, a Micro-Orifice Uniform Deposit Impactor (MOUDI)-120 sampler was used to collect size-resolved aerosol samples in three seasons in Beijing, and the concentrations of different types of carbonaceous fractions were analyzed. Furthermore, the size distribution, characteristics, sources, and interrelationship of each carbonaceous component in different seasons and under different pollution levels were systematically studied. The results show that the carbonaceous components were concentrated mainly in fine particles, and the proportion of carbonaceous components in fine particles in autumn and winter was higher than that in summer. The carbonaceous components are distributed in two main modes:accumulation mode and coarse mode. Organic carbon fraction 1 (OC1) and OC2 were distributed mainly in the accumulated mode, with a higher proportion in the range of 0.056-0.56 μm, and OC3+OC4 was more abundant in the coarse mode. The concentration of Soot-elemental carbon (EC) was low but was highest in the 0.10-0.18 μm size range, which indicates that the EC emitted by high temperature combustion was distributed mainly in the ultra-fine particle size range. The Char-EC concentration was much higher, accounting for the majority of EC. The distribution appearances of the main carbonaceous components were essentially the same in the daytime and at night. Summer and winter were more conducive to the formation of SOC, and the OC/EC ratio was significantly higher than that in autumn. The OC/EC values varied greatly in different particle sizes because the water-soluble organic compounds (WSOC) were distributed mainly in the range of 0.056-0.10 μm, with significantly higher OC/EC values than other particle sizes. Sunlight and high temperature were beneficial to the oxidation of gaseous organic matter to SOC, resulting in the OC/EC ratio in summer in daytime to be significantly higher than that at night. Among the carbonaceous components, EC1 and OC1 had the strongest interrelation. In addition, EC1 also had stronger interrelation with potassium.
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http://dx.doi.org/10.13227/j.hjkx.201903057DOI Listing
September 2019

Interaction of BDNF and cytokines in executive dysfunction in patients with chronic schizophrenia.

Psychoneuroendocrinology 2019 10 12;108:110-117. Epub 2019 Jun 12.

CAS Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences; Department of Psychology, University of Chinese Academy of Sciences, Beijing, China.

Multiple lines of evidence indicate that patients with chronic schizophrenia (SCZ) display executive dysfunction across the illness course. However, the potential molecular pathophysiologic mechanisms remain poorly elucidated. Neurodevelopmental changes caused by alterations of inflammatory mediators and neurotrophins have been shown to occur in the earliest stages of SCZ, and be associated with executive dysfunction (ED) in SCZ. Therefore, the current study was to investigate whether the interplay between BDNF and inflammatory mediators was involved in the disruption of executive function of long-term hospitalized patients with chronic SCZ. Serum cytokines and BDNF levels were measured in 112 long-term hospitalized patients with chronic SCZ and 44 healthy normal controls. Executive functions were assessed by verbal fluency tests (VFT), the Stroop word-color test (Stroop), and the Wisconsin card sorting tests (WCST).The results showed that the patients had higher IL-2, IL-6, IL-8, but lower TNF-α and BDNF compared to control subjects. In the patient group, BDNF was positively associated with IL-2 and IL-8 levels, while lower BDNF levels were correlated with ED measured by VFT and WCST tests. Multiple stepwise regression analyses confirmed that BDNF × IL-8 and BDNF × TNF-α were factors influencing the total score of VFT, while BDNF × IL-8 and BDNF × TNF-α were recognized as influencing factors for WCST scores. Our results suggest complex interactions between BDNF and cytokines were involved in the pathophysiology of executive function impairments in patients with SCZ.
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http://dx.doi.org/10.1016/j.psyneuen.2019.06.006DOI Listing
October 2019

Estimated Average Glandular Dose for 1,828 Mammography Procedures in China: A Multicenter Study.

Biomed Environ Sci 2019 Apr;32(4):242-249

Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 210009, Jiangsu, China.

Objective: To understand the distribution of the average glandular dose (AGD) in mammography by investigating 1,828 exposure parameters of 8 mammography machines in three cities, by using random sampling.

Methods: A survey of 8 mammography machines in three different cities, sampled using stratified random sampling methods, was performed, and 1,828 mammography exposure parameters were recorded. Incident air kerma (k) was measured by Quality-Assurance (QA) dosimeters, and AGD was calculated by series conversion coefficients based on a 3D detailed Monte Carlo breast model, published by Wang et al. RESULTS: The distribution of compressed breast thickness (CBT) fitted a normal distribution, while that of AGD fitted a skewed distribution. The mean value of CBT in a medio-lateral oblique (MLO) view was about 5.6% higher than that in the craniocaudal (CC) view, with significant statistical difference; mean value of AGD and CBT in the sample was 1.3 mGy and 4.6 cm, respectively. The AGD trended upward with increasing CBT, similar to the results of other researches.

Conclusion: The mean AGD and CBT levels in our study for mammography practice in China were 1.3 mGy and 4.6 cm, respectively. AGD is influenced by manufacturer-specific variation as machine response to CBT changes and target/filter combination. The present study can provide evidence for establishing a diagnostic reference level in China.
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http://dx.doi.org/10.3967/bes2019.034DOI Listing
April 2019
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