Publications by authors named "Dan Zhang"

1,792 Publications

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SGLT2 promotes pancreatic cancer progression by activating the Hippo signaling pathway via the hnRNPK-YAP1 axis.

Cancer Lett 2021 Jul 24. Epub 2021 Jul 24.

Department of Pancreatic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China; Sino-German Laboratory of Personalized Medicine for Pancreatic Cancer, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. Electronic address:

SGLT2 is overexpressed in various cancers, including pancreatic cancer. However, the mechanisms underlying the tumorigenic effects of SGLT2 in pancreatic cancer remain unclear. In this study, we demonstrated that SGLT2 inhibition significantly suppressed the growth of pancreatic cancer cells in vitro and in vivo. RNA sequencing, real-time PCR, and Western blot analyses revealed that SGLT2 silencing or inhibition suppressed Hippo signaling activation by downregulating YAP1 expression. Liquid chromatography-mass spectrometry and immunoprecipitation analyses showed that SGLT2 interacted with hnRNPK, promoting its nuclear translocation and thereby enhancing hnRNPK-induced YAP1 transcription. Importantly, YAP1 inhibitor enhanced the anti-pancreatic cancer effect of SGLT2 inhibitor in mice bearing pancreatic tumors. These findings suggest that SGLT2 promotes pancreatic cancer progression by activating the Hippo signaling pathway through the hnRNPK-YAP1 axis. Hence, SGLT2 inhibition alone or combined with YAP1 inhibition may represent a promising therapeutic approach for pancreatic cancer.
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http://dx.doi.org/10.1016/j.canlet.2021.07.035DOI Listing
July 2021

Dual-enzymatically cross-linked gelatin hydrogel promotes neural differentiation and neurotrophin secretion of bone marrow-derived mesenchymal stem cells for treatment of moderate traumatic brain injury.

Int J Biol Macromol 2021 Jul 24;187:200-213. Epub 2021 Jul 24.

School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, PR China. Electronic address:

Traumatic brain injury (TBI) is one of the most devastating nervous injuries. Neural tissue engineering based on stem cells and bioactive scaffold is a promising but challenging approach for neural repair. A cutting-edge system with capability to control the fate of encapsulated stem cells is attractive to enhance neural regeneration after TBI. Herein, an injectable gelatin hydrogel dual-enzymatically cross-linked by horse radish peroxidase (HRP) and choline oxidase (ChOx) was performed as the neural scaffold to load murine bone marrow-derived mesenchymal stem cells (BMSC) for TBI treatment. The results of in vitro cellular experiments showed that low cross-linked gelatin hydrogel could obviously promote cellular viability, neural differentiation, and neurotrophins secretion of the loaded BMSC. In vivo tests on a TBI model of C57BL/6 mouse demonstrated that BMSC-laden gelatin hydrogel implants could significantly reduce the damaged area, ameliorate inflammation, attenuate neuronal apoptosis, facilitate survival and proliferation of endogenous neural cells, and promote the neurological function recovery of TBI mice. All data suggest that establishment of this three-dimensional (3D) gelatin hydrogel stem cell-loaded system is a promising therapeutic strategy for TBI or other neurological rehabilitation.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.07.111DOI Listing
July 2021

[Pollution Characteristics and Source Apportionment of Atmospheric VOCs During Ozone Pollution Period in the Main Urban Area of Chongqing].

Huan Jing Ke Xue 2021 Aug;42(8):3595-3603

Key Laboratory for Urban Atmospheric Environment Integrated Observation & Pollution Prevention and Control of Chongqing, Chongqing Research Academy of Eco-Environmental Sciences, Chongqing 401147, China.

In late August 2020, a period of O pollution occurred in the main urban area of Chongqing and lasted for approximately 2 weeks (till early September). Ambient air samples, collected using Summa Canisters and DNPH sampling columns at three observation sites in the main urban area, were used to study the composition, photochemical reaction activity, and source apportionment of volatile organic compounds (VOCs) during the period of O pollution. The results showed that the mean volume fraction of TVOCs in the main urban area of Chongqing during the observation period was 45.08×10, and the components were ranked by volume fraction in the following order:OVOCs, alkanes, halohydrocarbons, alkenes, aromatics, and alkynes. Formaldehyde, ethylene, and acetone made up the higher volume fraction of VOCs, together accounting for more than 30% of TVOCs. OVOCs and alkenes contributed more to · OH loss rate () and ozone formation potential (OFP) and were the key VOCs components for ozone generation. The main active species in the OVOCs component were formaldehyde, acetaldehyde, and acrolein; the main active species in the alkene component were isoprene, ethylene, and -butene. The ratio of xylene to ethylbenzene in VOCs was low, and they showed a significant correlation, indicating that the VOCs air mass in the main urban area was highly aging and affected by long-distance transmission from other areas. The source apportionment results of the PMF model showed five main sources of VOCs, namely secondary generation (27.67%), vehicle exhaust (26.56%), industrial emission (17.86%), plant (14.51%), and fossil fuel combustion (13.4%).
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http://dx.doi.org/10.13227/j.hjkx.202101119DOI Listing
August 2021

RNA-seq bulked segregant analysis combined with KASP genotyping rapidly identified PmCH7087 as responsible for powdery mildew resistance in wheat.

Plant Genome 2021 Jul 26:e20120. Epub 2021 Jul 26.

Institute of Pharmaceutical & Food Engineering, Shanxi Univ. of Chinese Medicine, Jingzhong, 030619, China.

Powdery mildew causes considerable yield losses in common wheat (Triticum aestivum L.) production. Mapping and cloning powdery mildew-resistant quantitative trait loci can benefit stable yield production by facilitating the breeding of resistant varieties. In this study, we used the powdery mildew resistance introgression line 'CH7087' (harboring the resistance gene PmCH7087) and developed a large F population and a corresponding F segregation population containing 2,000 family lines for molecular mapping of PmCH7087. Genetic analysis demonstrated that the resistance phenotype was controlled by a single dominant gene. According to the performance exhibited by the F lines, 50 resistant lines and 50 susceptible lines without phenotype segregation were chosen for pooling and bulked segregant RNA sequencing (BSR-Seq) analysis. A region spanning 42.77 Mb was identified, and genotyping of an additional 183 F lines with extreme phenotypes using 20 kompetitive allele-specific polymerase chain reaction (KASP) markers in the BSR-Seq mapping regions confirmed this region and narrowed it to 9.68 Mb, in which 45 genes were identified and annotated. Five of these transcripts harbored nonsynonymous single nucleotide polymorphisms between the two parents, with the transcripts of TraesCS2B01G302800 being involved in signal transduction. Furthermore, TraesCS2B01G302800.2 was annotated as the closest homologue of serine/threonine-protein kinase PBS1, a typical participant in the plant disease immune response, indicating that TraesCS2B01G302800 was the candidate gene of PmCH7087. Our results may facilitate future research attempting to improve powdery mildew resistance in wheat and to identify candidate genes for further verification and gene cloning.
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http://dx.doi.org/10.1002/tpg2.20120DOI Listing
July 2021

Metagenomic Sequencing Analysis of the Effects of Colistin Sulfate on the Pig Gut Microbiome.

Front Vet Sci 2021 2;8:663820. Epub 2021 Jul 2.

Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, China.

The gut microbiome plays important roles in maintaining host health, and inappropriate use of antibiotics can cause imbalance, which may contribute to serious disease. However, despite its promise, using metagenomic sequencing to explore the effects of colistin on gut microbiome composition in pig has not been reported. Herein, we evaluated the roles of colistin in gut microbiome modulation in pigs. Metagenomic analysis demonstrated that overall microbial diversity was higher in the colistin group compared with the control group. Antibiotic Resistance Genes Database analysis demonstrated that following colistin treatment, expression levels of , and were significantly upregulated, indicating that colistin may induce transformation of antibiotic resistance genes. Colistin also affected the microbiome distribution patterns at both genus and phylum levels. In addition, at the species level, colistin significantly reduced the abundance of , and and enhanced the abundance of and compared to the control group. Gene Ontology analysis demonstrated that following treatment with colistin, metabolic process, cellular process, and single-organism process were the dominant affected terms. Kyoto Encyclopedia of Genes and Genomes analysis showed that oxidative phosphorylation, protein processing in endoplasmic reticulum, various types of N-glycan biosynthesis, protein processing in endoplasmic reticulum, pathogenic infection, and mitogen-activated protein kinase signaling pathway-yeast were the dominant signaling pathways in the colistin group. Overall, our results suggested that colistin affects microbial diversity and may modulate gut microbiome composition in pig, potentially providing novel strategy or antibiotic rationalization pertinent to human and animal health.
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http://dx.doi.org/10.3389/fvets.2021.663820DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282896PMC
July 2021

Societal impact of research: a text mining study of impact types.

Scientometrics 2021 Jul 10:1-21. Epub 2021 Jul 10.

Department of Information Resources Management, Business School, Nankai University, 94 Weijin Road, Nankai District, Tianjin, 300071 China.

In addition to academic impact, researchers are increasingly concerned with understanding and demonstrating the practical impact of research outside academia. Several frameworks capturing key impact types have been developed based on project experiences, expert opinions, and surveys. This empirical study seeks to contribute to this development by identifying impact types documented in 6,882 case studies submitted to impact evaluation groups in Australia (Engagement and Impact Assessment) and the United Kingdom (Research Excellence Framework). The results of text mining indicate three emerging impact types that extend existing frameworks in terms of the recognition of new opportunities, the length of use, and experience improvement, thereby allowing a variety of researchers, not just those who address popular, short-term, and instrumental issues, to understand and demonstrate their practice impact.
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http://dx.doi.org/10.1007/s11192-021-04096-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8272605PMC
July 2021

Atractylenolide III induces apoptosis by regulating the Bax/Bcl-2 signaling pathway in human colorectal cancer HCT-116 Cells in vitro and in vivo.

Anticancer Drugs 2021 Jul 12. Epub 2021 Jul 12.

Department of Pharmacology, School of Pharmacy, Southwest Medical University Pharmaceutical Department of Traditional Chinese Medicine, School of Pharmacy, Southwest Medical University Department of Pharmacy, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China.

Atractylodes is the dry root of atractylodes macrocephala koidz and has been commonly used as a traditional Chinese medicine (TCM). Atractylenolide III, a main component of atractylodes, has displayed significant effects on anti-inflammation and anticancer. However, the effects of atractylenolide III on growth inhibition and apoptosis induction in colon cancer remain unclear. The results showed that atractylenolide III significantly inhibited the cell growth and induce cellular apoptosis in HCT-116 cells in a concentration dependence manner in vitro. Mechanistic studies further showed that atractylenolide III could regulate the Bax/Bcl-2 apoptotic signaling pathway through promoting the expression of proapoptotic related gene/proteins Bax, caspase-9 and caspase-3 but inhibiting the expression of antiapoptotic related gene/protein Bcl-2 in HCT-116 cells. Furthermore, atractylenolide III also significantly inhibited the tumor growth of HCT-116 tumor xenografts bearing in nude mice through inducing apoptosis by upregulation of the expressions of Bax, cleaved caspase-3 and p53 but downregulation of the expressions of Bcl-2 in HCT-116 tumor tissues in vivo. The studies may provide the scientific rationale for the understanding of the anticancer effect of atractylenolide III. Therefore, atractylenolide III may have the potential to be developed as a promising novel anticancer agent for the treatment of colorectal cancer clinically.
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http://dx.doi.org/10.1097/CAD.0000000000001136DOI Listing
July 2021

The Aqueous Extract of Hance Prevents Cholesterol Gallstone in Mice by Affecting the Intestinal Microflora.

J Microbiol Biotechnol 2021 Jul 15;31. Epub 2021 Jul 15.

College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing 400016, China.

With changes in dietary patterns, the proportion of high-fat and high-cholesterol foods in the daily diet has increased. As a result, the incidence rate of cholelithiasis among people is increasing rapidly. Many studies have reported on the crucial role that the intestinal microflora plays in the progression of gallstones. Although the whole herb of , a traditional Chinese medicine, has long been extensively used as a remedy for cholelithiasis in China, its effects on the intestinal microflora remain unknown. Hence, this study aimed to test the ability of the aqueous extract of (LAE) to prevent cholesterol gallstones (CGSs) in model animals by affecting the intestinal microflora. The effects of LAE on body weight, serum lipid profile, visceral organ indexes, and histomorphology were studied in male C57BL/6J mice, which were induced by a lithogenic diet. After the 8-week study, CGSs formation was greatly reduced after LAE treatment. LAE also reduced body weight gain and hyperlipidemia and restored the histomorphological changes. Moreover, the intestinal microflora exhibited significant variation. In the model group fed the lithogenic diet, the abundances of the genera , and decreased, but in contrast, dramatically increased compared with the control check group, which was fed a normal diet; the administration of LAE reversed these changes. These results imply that can be considered an efficient therapy for eliminating CGSs induced by a high-fat and high-cholesterol diet, which may be achieved by influencing the intestinal microflora.
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http://dx.doi.org/10.4014/jmb.2106.06043DOI Listing
July 2021

Phytoremediation of secondary saline soil by halophytes with the enhancement of γ-polyglutamic acid.

Chemosphere 2021 Jul 7;285:131450. Epub 2021 Jul 7.

College of Resources and Environment, Southwest University, Chongqing Key Laboratory of Agricultural Resources and Environment, Chongqing, 400715, China. Electronic address:

Soil salinization has severely affected the quality of tillage land in China, and most greenhouse soils in Shanghai suburb suffer from secondary salinization with high salinity levels dominated by Ca, Mg and NO. In this work, a sandy loam soil (Calcaric Fluvisols) contaminated by the above ions was selected as research object, and the binding conditions and abilities of γ-polyglutamic acid (γ-PGA) to water-soluble Ca and Mg in the soil were examined, and then pot experiments were conducted to remove Ca, Mg and NO by two halophytes (Sedum aizoon L., Sesbania cannabina Pers.) integrated with γ-PGA application. The results showed that under the conditions of adding 1000 mg Lγ-PGA (pH 7) and 25 °C, the binding efficiencies of Ca and Mg were 51.59% and 68.03%, respectively. Compared with Sesbania cannabina Pers., Sedum aizoon L. displayed better remediation performance for the soil with γ-PGA application in pot experiments, and the removal efficiencies of Ca, Mg and NO reached 93.25%, 94.78% and 84.26% after applying 1000 mg Lγ-PGA for 56 d, respectively. Moreover, γ-PGA application could mitigate salt stress and promote plant growth, and activate antioxidant defense systems. Compared with the control, 1000 mg Lγ-PGA application significantly increased plant height and fresh weight of Sedum aizoon L., and the removal efficiencies of Ca, Mg and NO increased by 45.48%, 82.62% and 69.91%, respectively. In the future, more in-depth mechanism of joint effect and field-scale investigation need to be further studied.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131450DOI Listing
July 2021

Senescent cells in cancer therapy: why and how to remove them.

Cancer Lett 2021 Jul 5;520:68-79. Epub 2021 Jul 5.

Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei Province, People's Republic of China; Key Laboratory of Hubei Province for Digestive System Diseases, Wuhan, Hubei Province, People's Republic of China. Electronic address:

Cellular senescence is a stress response that imposes a growth arrest on cancer and nonmalignant cells during cancer therapy. By secreting a plethora of proinflammatory factors collectively termed the senescence-associated secretory phenotype (SASP), therapy-induced senescent cells can promote tumorigenesis. Moreover, the SASP from senescent cells is also able to drive therapy resistance and mediate many adverse effects of cancer therapy. Because senescent cell production often occurs during cancer therapy, it is important to carefully consider these potential detrimental effects. Senotherapy, which refers to selective removal of senescent cells, has been proposed as a promising adjuvant approach to eliminate the adverse effects of senescent cells. Thus, in this review we summarize in detail the mechanisms by which senescent cells contribute to tumorigenesis and therapeutic resistance. Also, we thoroughly discuss the potential strategies regarding how to effectively circumvent the undesirable effects of therapy-induced senescent cells.
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http://dx.doi.org/10.1016/j.canlet.2021.07.002DOI Listing
July 2021

Leading indicators of mental representation in construction hazard recognition.

Int J Occup Saf Ergon 2021 Jul 6:1-38. Epub 2021 Jul 6.

Associate Professor. Department of Construction Management, School of Civil Engineering, Tsinghua University, Beijing 100084, China. Email:

AbstracHazard recognition is mainly a visual search and cognitive process. Mental representations of hazards may impact mental states of hazard recognition. We assessed the effects of critical indicators of mental presentations of construction hazards on prefrontal cortex activation, a proxy for the mental states of hazard recognition. Students participated in a hazard inspection experiment, with near-infrared spectroscopy (NIRS) used to record prefrontal cortex activation. The effects of critical indicators of the hazards' mental representations on prefrontal activation were analyzed. Results demonstrated that site familiarity, risk tolerance, and safety knowledge have significant effects on medial prefrontal activation for hazards at low visual clutter level. High levels of site familiarity and risk tolerance reduced medial prefrontal activation and saved cognitive resources. Theoretically, the findings supplement the knowledge of safety hazards' mental representations; and practically, the findings guide provision of individual-specific guidance for improving workers' hazard inspection performance.
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http://dx.doi.org/10.1080/10803548.2021.1952005DOI Listing
July 2021

Down-regulation of S100P induces apoptosis in endometrial epithelial cell during GnRH antagonist protocol.

Reprod Biol Endocrinol 2021 Jul 2;19(1):99. Epub 2021 Jul 2.

Department of Obstetrics and Gynecology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai, 200025, China.

Background: The gonadotropin-releasing hormone (GnRH) antagonist protocol for in vitro fertilization (IVF) often leads to lower pregnancy rates compared to the GnRH agonist protocol. Decreased endometrial receptivity is one reason for the lower success rate, but the mechanisms underlying this phenomenon remain poorly understood. The S100 calcium protein P (S100P) is a biomarker for endometrial receptivity. Both GnRH antagonist and S100P are involved in mediating cell apoptosis. However, the involvement of S100P in reduced endometrial receptivity during the GnRH antagonist protocol remains unclear.

Methods: Endometrial tissue was collected at the time of implantation window from patients undergoing the GnRH agonist (GnRH-a) or GnRH antagonist (GnRH-ant) protocols, as well as from patients on their natural cycles. Endometrial cell apoptosis and expression levels of S100P, HOXA10, Bax, and Bcl-2 were assessed. Ishikawa cells were cultured to evaluate the effects that GnRH antagonist exposure or S100P up- or down- regulation had on apoptosis.

Results: Endometrial tissue from patients in the GnRH-ant group showed elevated apoptosis and decreased expression of the anti-apoptotic marker Bcl-2. In addition, endometrial expression of S100P was significantly reduced in the GnRH-ant group, and expression of HOXA10 was lower. Immunofluorescence colocalization analysis revealed that S100P was mainly distributed in the epithelium. In vitro experiments showed that knockdown of S100P in Ishikawa cells induced apoptosis, decreased expression of Bcl-2, while overexpression of S100P caused the opposite effects and decreased expression of Bax. Furthermore, endometrial epithelial cells exposed to GnRH antagonist expressed lower levels of S100P and Bcl-2, increased expression of Bax, and had higher rates of apoptosis. The increased apoptosis induced by GnRH antagonist treatment could be rescued by overexpression of S100P.

Conclusions: We found that GnRH antagonist treatment induced endometrial epithelial cell apoptosis by down-regulating S100P, which was detrimental to endometrial receptivity. These results further define a mechanistic role for S100P in contributing to endometrial apoptosis during GnRH antagonist treatment, and suggest that S100P is a potential clinical target to improve the success of IVF using the GnRH antagonist protocol.
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http://dx.doi.org/10.1186/s12958-021-00787-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8252288PMC
July 2021

Generation of an induced pluripotent stem cell line from a patient with Stargardt disease caused by biallelic c.[5461-10T>C;5603A>T];[6077T>C] mutations in the ABCA4 gene.

Stem Cell Res 2021 Jul 24;54:102439. Epub 2021 Jun 24.

Ocular Tissue Engineering Laboratory, Lions Eye Institute, Nedlands, Western Australia, Australia; Centre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Australian Inherited Retinal Disease Registry and DNA Bank, Department of Medical Technology and Physics, Sir Charles Gairdner Hospital, Nedlands, Western Australia, Australia; Department of Ophthalmology, Royal Perth Hospital, Perth, Western Australia, Australia; Department of Ophthalmology, Perth Children's Hospital, Nedlands, Western Australia, Australia.

Mutations in ABCA4 gene are causative for autosomal recessive Stargardt disease (STGD1), the most common inherited retinal dystrophy. Here, we report the generation of an induced pluripotent stem cell (iPSC) line from a STGD1 patient carrying biallelic c.[5461-10T>C;5603A>T];[6077T>C] mutations in the ABCA4 gene. Episomes carrying OCT4, SOX2, KLF4, L-MYC, LIN28 and mp53DD were employed for the reprogramming of patient-derived fibroblasts. This iPSC line expressed comparable pluripotency markers as in a commercially available human iPSC line, displayed normal karyotype and potential for trilineage differentiation, and were negative for both reprogramming episomes and mycoplasma test.
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http://dx.doi.org/10.1016/j.scr.2021.102439DOI Listing
July 2021

Clinical Evidence for the Importance of the Wild-Type Allele in the Phenotypic Expression of RP11.

Genes (Basel) 2021 Jun 14;12(6). Epub 2021 Jun 14.

Centre for Ophthalmology and Visual Science, The University of Western Australia, Perth, WA 6009, Australia.

-associated retinopathy (RP11) is a common form of autosomal dominant retinitis pigmentosa (adRP) that exhibits wide variation in phenotype ranging from non-penetrance to early-onset RP. Herein, we report inter-familial and intra-familial variation in the natural history of RP11 using multimodal imaging and microperimetry. Patients were recruited prospectively. The age of symptom onset, best-corrected visual acuity, microperimetry mean sensitivity (MS), residual ellipsoid zone span and hyperautofluorescent ring area were recorded. Genotyping was performed using targeted next-generation and Sanger sequencing and copy number variant analysis. mutations were found in 14 individuals from seven unrelated families. Four disease patterns were observed: (A) childhood onset with rapid progression ( = 4), (B) adult-onset with rapid progression ( = 4), (C) adult-onset with slow progression ( = 4) and (D) non-penetrance ( = 2). Four different patterns were observed in a family harbouring c.267del; patterns B, C and D were observed in a family with c.772_773delins16 and patterns A, B and C were observed in 3 unrelated individuals with large deletions. Our findings suggest that the RP11 phenotype may be related to the wild-type allele rather than the type of mutation. Further studies that correlate in vitro wild-type allele expression level with the disease patterns are required to investigate this association.
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http://dx.doi.org/10.3390/genes12060915DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232116PMC
June 2021

Generation of two induced pluripotent stem cell lines from a patient with Stargardt disease caused by compound heterozygous mutations in the ABCA4 gene.

Stem Cell Res 2021 Jul 24;54:102448. Epub 2021 Jun 24.

Ocular Tissue Engineering Laboratory, Lions Eye Institute, Nedlands, Western Australia, Australia; Centre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia. Electronic address:

Stargardt disease (STGD1) is the most common inherited retinal dystrophy and ABCA4 c.546--10 T>C is the most commonly reported splice mutation. Here, we generated and characterized two induced pluripotent stem cell (iPSC) lines from a STGD1 patient with compound heterozygous mutations in ABCA4 (c.[5461-10 T > C;5603A > T];[4163 T > C;455G > A]). Episomal vectors containing OCT4, SOX2, KLF4, L-MYC, LIN28 and mp53DD were employed to conduct the reprogramming of patient-derived fibroblasts. Both lines had a normal karyotype, displayed iPSC morphology, expressed pluripotency markers and showed trilineage differentiation potential. These lines can provide a powerful platform for further investigating the pathophysiological consequences of mutations in ABCA4.
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http://dx.doi.org/10.1016/j.scr.2021.102448DOI Listing
July 2021

Generation of two induced pluripotent stem cell lines from a patient with Stargardt disease caused by compound heterozygous mutations in the ABCA4 gene.

Stem Cell Res 2021 Jul 24;54:102448. Epub 2021 Jun 24.

Ocular Tissue Engineering Laboratory, Lions Eye Institute, Nedlands, Western Australia, Australia; Centre for Ophthalmology and Visual Sciences, The University of Western Australia, Nedlands, Western Australia, Australia. Electronic address:

Stargardt disease (STGD1) is the most common inherited retinal dystrophy and ABCA4 c.546--10 T>C is the most commonly reported splice mutation. Here, we generated and characterized two induced pluripotent stem cell (iPSC) lines from a STGD1 patient with compound heterozygous mutations in ABCA4 (c.[5461-10 T > C;5603A > T];[4163 T > C;455G > A]). Episomal vectors containing OCT4, SOX2, KLF4, L-MYC, LIN28 and mp53DD were employed to conduct the reprogramming of patient-derived fibroblasts. Both lines had a normal karyotype, displayed iPSC morphology, expressed pluripotency markers and showed trilineage differentiation potential. These lines can provide a powerful platform for further investigating the pathophysiological consequences of mutations in ABCA4.
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http://dx.doi.org/10.1016/j.scr.2021.102448DOI Listing
July 2021

Effect of the traditional Chinese medicine Pinggan-Qianyang decoction on SIRT1-PTEN signaling in vascular aging in spontaneously hypertensive rats.

Hypertens Res 2021 Jun 29. Epub 2021 Jun 29.

International Medical Center, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, P.R. China.

Age-related functional decline is a physiological phenomenon that occurs in all organ systems. However, the acceleration and early occurrence of this process are observed in cardiovascular pathologies, including hypertension. This study aimed to investigate SIRT1-PTEN signaling in aortic tissue from spontaneously hypertensive rats (SHRs) and changes in SIRT1 and PTEN expression following treatment with Pinggan-Qianyang decoction (PGQYD) and explore the mechanism involved in the treatment of hypertensive vascular aging with traditional Chinese medicine. In this study, we used two rat models: spontaneously hypertensive rats (SHRs) at 14 and 64 weeks of age and WKY rats at 64 weeks of age. The degree of irritability and rotation tolerance time were evaluated to determine the effects of PGQYD on animal behavior. The morphology of the thoracic aorta was examined by hematoxylin-eosin (HE) and Masson staining and electron microscopy. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity and superoxide dismutase (SOD) and anti-superoxide anion content were detected. Senescence-associated β-galactosidase (SA-β-Gal) staining was used to observe the thoracic aorta during vascular aging. RT-qPCR, immunofluorescence, and Western blot analysis were performed to detect changes in the mRNA and protein expression of p53, p21, SIRT1, and PTEN in rat aortic tissues. Behavioral tests and histological and morphological analyses showed the remarkable amelioration of vascular aging after PGQYD treatment compared with that in the older SHRs. Moreover, PGQYD significantly decreased vascular aging in SHRs, as indicated by reduced SA-β-Gal staining, NADPH oxidase activity, and p53 and p21 expression, and increased anti-superoxide anion and SOD content. Furthermore, PGQYD increased SIRT1 and PTEN expression, but the downregulated expression of SIRT1 induced by a SIRT1 inhibitor abolished the PGQYD-induced antiaging effects on gene expression and antioxidant activity and enhanced PTEN expression. PGQYD could ameliorate vascular aging effects in SHRs, which may have been mediated via the regulation of SIRT1-PTEN signaling in aortic tissue.
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http://dx.doi.org/10.1038/s41440-021-00682-6DOI Listing
June 2021

Solvent-free microwave synthesis of ultra-small [email protected] with strong metal-support interaction for industrial hydrogen evolution.

Nat Commun 2021 Jun 29;12(1):4018. Epub 2021 Jun 29.

Key Laboratory of Eco-chemical Engineering, Key Laboratory of Optic-electric Sensing and Analytical Chemistry of Life Science, Taishan Scholar Advantage and Characteristic Discipline Team of Eco Chemical Process and Technology, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, P. R. China.

Exploring a simple, fast, solvent-free synthetic method for large-scale preparation of cheap, highly active electrocatalysts for industrial hydrogen evolution reaction is one of the most promising work today. In this work, a simple, fast and solvent-free microwave pyrolysis method is used to synthesize ultra-small (3.5 nm) [email protected] catalyst with heterogeneous structure and strong metal-support interaction in one step. The [email protected] catalyst only exhibits an overpotential of 15 mV at a current density of 10 mA cm, and exhibits a large turnover frequency value up to 21.9 s under an overpotential of 100 mV in 1.0 M KOH. In addition, this catalyst can reach high current densities of 500 mA cm and 1000 mA cm at low overpotentials of 56 mV and 78 mV respectively, and it displays high stability of 1000 h. This work provides a feasible way for the reasonable design of other large-scale production catalysts.
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http://dx.doi.org/10.1038/s41467-021-24322-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8242096PMC
June 2021

Snail enhances arginine synthesis by inhibiting ubiquitination-mediated degradation of ASS1.

EMBO Rep 2021 Jun 29:e51780. Epub 2021 Jun 29.

Tongren Hospital/Faculty of Basic Medicine, Hongqiao International Institute of Medicine, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Snail is a dedicated transcriptional repressor and acts as a master inducer of EMT and metastasis, yet the underlying signaling cascades triggered by Snail still remain elusive. Here, we report that Snail promotes colorectal cancer (CRC) migration by preventing non-coding RNA LOC113230-mediated degradation of argininosuccinate synthase 1 (ASS1). LOC113230 is a novel Snail target gene, and Snail binds to the functional E-boxes within its proximal promoter to repress its expression in response to TGF-β induction. Ectopic expression of LOC113230 potently suppresses CRC cell growth, migration, and lung metastasis in xenograft experiments. Mechanistically, LOC113230 acts as a scaffold to facilitate recruiting LRPPRC and the TRAF2 E3 ubiquitin ligase to ASS1, resulting in enhanced ubiquitination and degradation of ASS1 and decreased arginine synthesis. Moreover, elevated ASS1 expression is essential for CRC growth and migration. Collectively, these findings suggest that TGF-β and Snail promote arginine synthesis via inhibiting LOC113230-mediated LRPPRC/TRAF2/ASS1 complex assembly and this complex can serve as potential target for the development of new therapeutic approaches to treat CRC.
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http://dx.doi.org/10.15252/embr.202051780DOI Listing
June 2021

A dataset of daily ambulatory psychological and physiological recording for emotion research.

Sci Data 2021 06 28;8(1):161. Epub 2021 Jun 28.

Department of Psychology, School of Social Sciences, Tsinghua University, Beijing, China.

To better understand the psychological and physiological basis of human emotion, increasing interest has been drawn towards ambulatory recordings of emotion-related data beyond the laboratories. By employing smartphones-based ambulatory assessment and wrist-worn physiological recording devices, the Daily Ambulatory Psychological and Physiological recording for Emotion Research (DAPPER) dataset provides momentary self-reports and physiological data of people's emotional experiences in their daily life. The dataset consists of ambulatory psychological recordings from 142 participants and physiological recordings from 88 of them over five days. Both the experience sampling method (ESM) and the day reconstruction method (DRM) were employed to have a comprehensive description of the participants' daily emotional experiences. Heart rate, galvanic skin response, and three-axis acceleration were recorded during the day time. By including multiple types of physiological and self-report data at a scale of five days with 100+ participants, the present dataset is expected to promote emotion researches in real-life, daily settings.
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http://dx.doi.org/10.1038/s41597-021-00945-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8239004PMC
June 2021

A new triarylindanone and a new isobenzofuranone derivative from .

Nat Prod Res 2021 Jun 28:1-6. Epub 2021 Jun 28.

Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan, PR China.

A new triarylindanone, namely selagindanone A (), and a new isobenzofuranone (), 3,4-bis(4-hydroxyphenyl)isobenzofuran-1(3)-one, were isolated from . Their structures were elucidated by comprehensive spectroscopic and mass spectrometric analyses, including 1 D-, 2 D-NMR and HR-ESI-MS. Compound possesses a unique structural feature of triaryl-substituted in the skeleton of 1-indanone. In addition, compound showed weak cytotoxicity against human hepatocellular carcinoma SMMC-7721 and HepG2 cell lines.
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http://dx.doi.org/10.1080/14786419.2021.1938042DOI Listing
June 2021

Oligo/Amenorrhea Is an Independent Risk Factor Associated With Low Ovarian Response.

Front Endocrinol (Lausanne) 2021 9;12:612042. Epub 2021 Jun 9.

Key Laboratory of Reproductive Genetics (Ministry of Education) and Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Capsule: Oligo/amenorrhea is an independent risk factor of low ovarian response but not high ovarian response, particularly in women with low AMH levels.

Objective: To investigate the association of menstrual cycle length (MCL) with anti-Müllerian hormone (AMH) and ovarian response.

Methods: This was a retrospective cohort study. A total of 7471 women who underwent ovarian stimulation and oocyte retrieval were enrolled. The main outcome was the number of oocytes retrieved.

Main Results: A total of 5734 patients were eligible for analysis. In women without polycystic ovary syndrome (PCOS), serum AMH levels and antral follicle count were significantly lower in women with short cycles and higher in women with oligo/amenorrhea than those with a normal menstrual cycle. In women with PCOS, compared to women with a normal menstrual cycle, women with short cycles and women with oligo/amenorrhea showed higher antral follicle count and higher serum AMH levels. Compared with the 0-25th range group of AMH levels, 75-100th percentile groups showed a significantly increased rate of oligo/amenorrhea in women with and without PCOS [adjusted odds ratio (OR) =1.9 (1.04, 3.46), 2.4 (1.70, 3.35)]. In women without PCOS, the low ovarian response was more common in women with short cycles and less common in women with oligo/amenorrhea compared to women with normal cycles [OR=3.0 (2.38, 3.78), 0.7 (0.55, 0.96), respectively]. When adjusted for AMH levels, both short cycles and oligo/amenorrhea were associated with an increased risk of low response [adjusted OR=1.3 (1.02, 1.75), 1.3 (0.93, 1.86), respectively]. In women without PCOS and with low AMH levels, the low ovarian response was more common in women with short cycles as well as in women with oligo/amenorrhea [OR=1.5 (1.08, 1.98), 1.7 (1.08, 2.69), adjusted OR=1.2 (0.86, 1.74), 2.2 (1.31, 3.82), respectively].

Conclusion: AMH levels are significantly associated with increased risk of oligo/amenorrhea in women with and without PCOS. AMH is an indispensable confounder in the association between MCL and ovarian response in women without PCOS. Oligo/amenorrhea is an independent risk factor associated with a low ovarian response in women without PCOS, particularly those with low AMH levels.
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http://dx.doi.org/10.3389/fendo.2021.612042DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220146PMC
June 2021

Value of preoperative gastroscopic carbon nanoparticles labeling in patients undergoing laparoscopic radical gastric cancer surgery.

Surg Oncol 2021 Jun 23;38:101628. Epub 2021 Jun 23.

Department of Gastroenterology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaan Xi, 710061, PR China. Electronic address:

Background And Objectives: Lymph node metastasis is crucial for gastric cancer. We aim to explore the value of preoperative gastroscopic carbon nanoparticles labeling in patients undergoing laparoscopic radical gastric cancer surgery.

Methods: 1199 cases undergoing laparoscopic radical gastric cancer surgery were retrospectively analyzed. 214 cases received preoperative gastroscopic carbon nanoparticles labeling. The number of total lymph nodes detected, positive lymph nodes, N staging, and operation time were analyzed.

Results: The patients received carbon nanoparticles labeling had more total lymph nodes detected (27.9 ± 6.5 vs 22.2 ± 4.0; P < 0.001) and shorter operation time (185.9 ± 27.8min vs 218.7 ± 69.2min; P < 0.001) compared with the control group. In addition, preoperative endoscopic carbon nanoparticles labeling improved the diagnosis rate of synchronous multiple gastric cancer (2.8% vs 0.4%; P < 0.001).

Conclusions: Preoperative endoscopic carbon nanoparticles tracer labeling is of great value in patients undergoing laparoscopic radical gastric cancer surgery. It shortens the operation time, increases the number of total lymph nodes detected for more accurate pathological TNM staging, and finds some cases of synchronous multiple gastric cancer.
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http://dx.doi.org/10.1016/j.suronc.2021.101628DOI Listing
June 2021

Assessment of the Diagnostic Ability of Four Detection Methods Using Three Sample Types of COVID-19 Patients.

Front Cell Infect Microbiol 2021 7;11:685640. Epub 2021 Jun 7.

Department of Laboratory Medicine, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: Viral nucleic acid detection is considered the gold standard for the diagnosis of coronavirus disease 2019 (COVID-19), which is caused by SARS-CoV-2 infection. However, unsuitable sample types and laboratory detection kits/methods lead to misdiagnosis, which delays the prevention and control of the pandemic.

Methods: We compared four nucleic acid detection methods [two kinds of reverse transcription polymerase chain reactions (RT-PCR A: ORF1ab and N testing; RT-PCRB: only ORF1ab testing), reverse transcription recombinase aided amplification (RT-RAA) and droplet digital RT-PCR (dd-RT-PCR)] using 404 samples of 72 hospitalized COVID-19 patients, including oropharyngeal swab (OPS), nasopharyngeal swabs (NPS) and saliva after deep cough, to evaluate the best sample type and method for SARS-CoV-2 detection.

Results: Among the four methods, dd-RT-PCR exhibited the highest positivity rate (93.0%), followed by RT-PCR B (91.2%) and RT-RAA (91.2%), while the positivity rate of RT-PCR A was only 71.9%. The viral load in OPS [24.90 copies/test (IQR 15.58-129.85)] was significantly lower than that in saliva [292.30 copies/test (IQR 20.20-8628.55)] and NPS [274.40 copies/test (IQR 33.10-2836.45)]. In addition, if OPS samples were tested alone by RT-PCR A, only 21.4% of the COVID-19 patients would be considered positive. The accuracy of all methods reached nearly 100% when saliva and NPS samples from the same patient were tested simultaneously.

Conclusions: SARS-CoV-2 nucleic acid detection methods should be fully evaluated before use. High-positivity rate methods such as RT-RAA and dd-RT-PCR should be considered when possible. Furthermore, saliva after deep cough and NPS can greatly improve the accuracy of the diagnosis, and testing OPS alone is not recommended.
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http://dx.doi.org/10.3389/fcimb.2021.685640DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8216554PMC
June 2021

Piperidine-Mediated [3 + 3] Cyclization of 2-Amino-4-chromen-4-ones and 2-Benzylidenemalononitriles: To Access 2-Aminochromeno[2,3-]pyridine Derivatives.

J Org Chem 2021 Jul 23;86(13):9218-9224. Epub 2021 Jun 23.

School of Chemistry and Chemical Engineering, Yangzhou University,180 Siwangting Street, Yangzhou 225002, P. R. China.

Piperidine-mediated [3 + 3] cyclization of 2-amino-4-chromen-4-ones and substituted 2-benzylidenemalononitriles was developed for the synthesis of 2-amino-4-aryl-5-chromeno[2,3-]pyridin-5-one derivatives. This novel transformation provides a highly efficient and facile route to functionalized 5-chromeno[2,3-]pyridines from readily available substrates under mild reaction conditions.
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http://dx.doi.org/10.1021/acs.joc.1c00797DOI Listing
July 2021

A Novel Nitrogen and Carbon Metabolism Regulatory Cascade Is Implicated in Entomopathogenicity of the Fungus Metarhizium robertsii.

mSystems 2021 Jun 22;6(3):e0049921. Epub 2021 Jun 22.

MOE Key Laboratory of Biosystems Homeostasis & Protection, Institute of Microbiology, College of Life Science, Zhejiang Universitygrid.13402.34, Hangzhou, China.

The entomopathogenic fungus Metarhizium robertsii can switch among parasitic, saprophytic, and symbiotic lifestyles in response to changing nutritional conditions, which is attributed to its extremely versatile metabolism. Here, we found that the Fus3-mitogen-activated protein kinase (MAPK) and the transcription factor regulator of nutrient selection 1 (RNS1) constitute a novel fungal cascade that regulates the degradation of insect cuticular lipids, proteins, and chitin to obtain nutrients for hyphal growth and enter the insect hemocoel for subsequent colonization. On the insect cuticle, Fus3-MAPK physically contacts and phosphorylates RNS1, which facilitates the entry of RNS1 into nuclei. The phosphorylated RNS1 binds to the DNA motif (ACCAGAC) in its own promoter to self-induce expression, which then activates the expression of genes for degrading cuticular proteins, chitin, and lipids. We further found that the Fus3-MAPK/RNS1 cascade also activates genes for utilizing complex and less-favored nitrogen and carbon sources (casein, colloid chitin, and hydrocarbons) that were not derived from insects, which is repressed by favored organic carbon and nitrogen nutrients, including glucose and glutamine. In conclusion, we discovered a novel regulatory cascade that controls fungal nitrogen and carbon metabolism and is implicated in the entomopathogenicity of . Penetration of the cuticle, the first physical barrier to pathogenic fungi, is the prerequisite for fungal infection of insects. In the entomopathogenic fungus Metarhizium robertsii, we found that the Fus3-mitogen-activated protein kinase (MAPK) and the transcription factor regulator of nutrient selection 1 (RNS1) constitute a novel cascade that controls cuticle penetration by regulating degradation of cuticular lipids, proteins, and chitin to obtain nutrients for hyphal growth and entry into the insect hemocoel. In addition, during saprophytic growth, the Fus3-MAPK/RNS1 cascade also activates utilization of complex and less-favored carbon and nitrogen sources that are not derived from insects. The homologs of Fus3-MAPK and RNS1 are widely found in ascomycete filamentous fungi, including saprophytes and pathogens with diverse hosts, suggesting that the regulation of utilization of nitrogen and carbon sources by the Fus3-MAPK/RNS1 cascade could be widespread. Our work provides significant insights into regulation of carbon and nitrogen metabolism in fungi and fungal pathogenesis in insects.
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http://dx.doi.org/10.1128/mSystems.00499-21DOI Listing
June 2021

New frontiers against sorafenib resistance in renal cell carcinoma: From molecular mechanisms to predictive biomarkers.

Pharmacol Res 2021 Jun 15;170:105732. Epub 2021 Jun 15.

State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu 610041, PR China. Electronic address:

Renal cell carcinoma (RCC) is a highly vascularized tumor and prone to distant metastasis. Sorafenib is the first targeted multikinase inhibitor and first-line chemical drug approved for RCC therapy. In fact, only a small number of RCC patients benefit significantly from sorafenib treatment, while the growing prevalence of sorafenib resistance has become a major obstacle for drug therapy effectivity of sorafenib. The molecular mechanisms of sorafenib resistance in RCC are not completely understood by now. Herein, we comprehensively summarize the underlying mechanisms of sorafenib resistance and molecular biomarkers for predicting sorafenib responsiveness. Moreover, we outline strategies suitable for overcoming sorafenib resistance and prospect potential approaches for identifying biomarkers associated with sorafenib resistance in RCC, which contributes to guide individualized and precision drug therapy.
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http://dx.doi.org/10.1016/j.phrs.2021.105732DOI Listing
June 2021

A new type of zinc ion hybrid supercapacitor based on 2D materials.

Nanoscale 2021 Jul;13(25):11004-11016

Shaanxi Province Key Laboratory of Catalytic Foundation and Application, School of Chemistry and Environment Science, Shaanxi University of Technology, Hanzhong 723001, China.

Zinc ion hybrid supercapacitors (ZICs) are truly promising competitors in prospective extensive electrochemical energy storage fields due to their cost-effectiveness, environmentally friendly nature, inherent security, and satisfying gravimetric energy density. Thus, several investigation endeavors have been dedicated to the construction and exploitation of high-performance ZICs. However, the exploitation of ZICs is still in its preliminary stage and there are many problems that need to be overcome before their potential can be fully realized. Recently, 2D materials with a fascinating structure and intriguing features have attracted enormous attention for applications in ZICs with prominent improvement from charge storage capacity to reaction kinetics. In this article, the recent research progress in 2D materials and their composites in the pursuit of high-performance ZICs is systematically reviewed, focusing on the possible charge storage mechanism of ZICs. In addition, the influence of the structure of 2D materials and their composites on the electrochemical performance and the zinc ion storage mechanism is analyzed. Finally, the challenges and prospects of the application of 2D materials and their composites in high-performance ZICs are presented.
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http://dx.doi.org/10.1039/d1nr03215aDOI Listing
July 2021

A Clinical Analysis of the Treatment of Chronic Coronary Artery Occlusion With Antegrade Dissection Reentry.

Front Surg 2021 31;8:609403. Epub 2021 May 31.

Department of Cardiology, Binzhou People's Hospital Affiliated to Shandong First Medical University, Binzhou, China.

This study aimed to investigate the efficacy and safety of antegrade dissection re-entry (ADR) technique in the percutaneous coronary intervention (PCI) to open chronic total occlusion (CTO) lesions. The baseline, angiographic results, PCI success rate, and major adverse cardiac events (MACE) during the 12 months of follow-up were compared between 48 patients who did not use ADR in the treatment of CTO lesions (control group) and 50 patients who used ADR (treatment group). The control group comprised 48 patients who had 52 CTO lesions, and the treatment group comprised 50 patients who had 58 CTO lesions. The success rate of PCI in the treatment group (89.7 vs. 71.2%, = 0.047) was significantly higher than in the control group, where six patients had in-stent restenosis (ISR, ISR-CTO) that were all recanalized. The mean PCI time (71 ± 25 min vs. 95 ± 33 min, = 0.041), X-ray exposure time (42 ± 17 min vs. 71 ± 22 min, = 0.032), contrast agent dosage (98 ± 26 ml vs. 178 ± 63 ml, = 0.029), MACE incidence during the 12 months of follow-up (22.0 vs. 41.7%, = 0.046) and recurrent myocardial infarction incidence (10.0 vs. 27.1%, = 0.047) were significantly lower in the treatment group than in the control group. The differences were all statistically significant. It is safe and effective to use the ADR technique in PCI for coronary artery CTO lesions. The technique shortens the operation time, reduces the radiation dose of doctors and patients and the use dose of contrast agents, and improves patients' prognoses.
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http://dx.doi.org/10.3389/fsurg.2021.609403DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8200522PMC
May 2021

Transcriptome profiling reveals the spatial-temporal dynamics of gene expression essential for soybean seed development.

BMC Genomics 2021 Jun 16;22(1):453. Epub 2021 Jun 16.

Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Zhengzhou, 450002, China.

Background: Seeds are the economic basis of oilseed crops, especially soybeans, the most widely cultivated oilseed crop worldwide. Seed development is accompanied by a multitude of diverse cellular processes, and revealing the underlying regulatory activities is critical for seed improvement.

Results: In this study, we profiled the transcriptomes of developing seeds at 20, 25, 30, and 40 days after flowering (DAF), as these stages represent critical time points of seed development from early to full development. We identified a set of highly abundant genes and highlighted the importance of these genes in supporting nutrient accumulation and transcriptional regulation for seed development. We identified 8925 differentially expressed genes (DEGs) that exhibited temporal expression patterns over the course and expression specificities in distinct tissues, including seeds and nonseed tissues (roots, stems, and leaves). Genes specific to nonseed tissues might have tissue-associated roles, with relatively low transcript abundance in developing seeds, suggesting their spatially supportive roles in seed development. Coexpression network analysis identified several underexplored genes in soybeans that bridge tissue-specific gene modules.

Conclusions: Our study provides a global view of gene activities and biological processes critical for seed formation in soybeans and prioritizes a set of genes for further study. The results of this study help to elucidate the mechanism controlling seed development and storage reserves.
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http://dx.doi.org/10.1186/s12864-021-07783-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8207594PMC
June 2021
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