Publications by authors named "Dan Xiao"

346 Publications

One-step fast and label-free imaging array for multiplexed detection of trace avian influenza viruses.

Anal Chim Acta 2021 Aug 17;1171:338645. Epub 2021 May 17.

College of Chemistry, Sichuan University, Chengdu, 610064, PR China. Electronic address:

Rapid and low-cost diagnosis of multiple infectious diseases is of great significance especially in densely populated or resource-constrained settings. Herein, we developed a one-step fast and label-free imaging array for multiplexed detection of trace avian influenza virus (AIV) DNA biomarkers. By designing a series of specific and efficient catalytic hairpin assembly (CHA) amplification reactions and utilizing thioflavin T, a specific G-quadruplex fluorescence probe, three subtypes of AIV DNA biomarkers (H1N1, H7N9 and H5N1) were simultaneously and quickly detected within only 20 min, which just needed a small reagent volume of 50 μL and a smartphone instead of a spectrometer. With the combination of fluorescence imaging output and grey-level analysis, the array sensor can be on-site with the limit of detection of 136 pM, 141 pM and 129 pM for H1N1, H7N9 and H5N1, respectively. The imaging array also displayed good mismatch discrimination, excellent anti-interference, and real sample application. In view of its advantages of fast detection, low cost and multiplexed analysis, the imaging array is expected to have potential applications for early infectious disease diagnosis in resource-constrained settings.
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http://dx.doi.org/10.1016/j.aca.2021.338645DOI Listing
August 2021

Impact of Chinese Respiratory Physicians Participating in Smoking Cessation and Mobile Health: A Randomized Feasibility Trial.

Clin Respir J 2021 Jun 4. Epub 2021 Jun 4.

Department of Gerontology and Geriatrics, The First Hospital of China Medical University, Shenyang, China.

Introduction: People who are eager to quit smoking often lack long-term, daily smoking cessation guidance. In addition, advances in mobile communication technology offer promising ways for providing tobacco dependence treatment. However, it is unclear whether the doctor-WeChat network can improve the smoking cessation rate of nicotine-dependent patients.

Methods: In this prospective single-blind cohort study, 250 smokers were enrolled from May 2018 to October 2018. They were randomly divided into two groups, with or without doctors' active smoking cessation service, and followed up for 6 months. The smoking cessation rate and characteristics of successful smoking cessation groups were compared. The reasons for relapse were also analyzed.

Results: After smoking cessation for 3 months, the success rate of the group involving active respiratory physicians was 65.0% (80/123); the success rate of the control group was 34.7% (34/98). After 6 months, the success rate of the group involving active respiratory physicians was 55.3% (68/123), while that of the control group was only 11.2% (11/98). There was no difference in the weight change of the participants between the two groups. Subgroup analysis showed that doctors' participation had a greater impact on the success of smoking cessation on men younger than 45 years or unemployed.

Conclusions: Doctors in mobile smoking cessation services played a very important role in improving quit rates. Our research provided methodological guidance for further clinical trials and a template for further real-world applications of smoking cessation services.
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http://dx.doi.org/10.1111/crj.13404DOI Listing
June 2021

Rapid synthesis of fluorescent bovine serum albumin-gold nanoclusters complex for glutathione determination.

Authors:
Ting Wang Dan Xiao

Mikrochim Acta 2021 May 19;188(6):193. Epub 2021 May 19.

College of Chemistry, Sichuan University, Chengdu, 610064, People's Republic of China.

A facile one-pot method for synthesis of bovine serum albumin (BSA)-gold nanoclusters (AuNCs) has been developed. The formation of BSA-AuNCs took only 30 s under mild conditions. BSA-AuNCs exhibited strong orange-yellow fluorescence, and the excitation and emission peaks were at 370 nm and 564 nm, respectively. In the process of forming BSA-AuNCs, the molecular chain of BSA has not been destroyed. Moreover, there were a large number of Au cations on the surface of BSA-AuNCs, which had strong oxidizing abilities. The reason for the ultrabright fluorescence of BSA-AuNCs was attributed to the Au(0)@Au(I)@Au(III)-ligand structure on the surface of BSA. In order to evaluate the fluorescence performance of BSA-AuNCs, BSA-AuNCs was used as a probe, realizing the sensitive and selective determination of glutathione (GSH) in a wide linear range of 0.01-0.48 μM and a detection limit of 3.3 nM. The proposed method not only offers a brand-new scheme for synthesizing BSA-AuNCs, but also provides a platform for studying the interaction between metal core and proteins. A facile one-pot method to synthesize ultrabright fluorescent BSA-AuNCs in tens of seconds has been introduced by mixing BSA suspension, KSCN, and HAuCl. The as-prepared BSA-AuNCs showed intensive orange-yellow fluorescence under a UV lamp (365 nm) and BSA still keeps the integral molecular chains during the whole synthesis process. Moreover, the as-prepared BSA-AuNCs have realized the sensitive and selective detection of glutathione (GSH) in a wide linear range of 0.01-0.48 μM and a detection limit of 3.3 nM.
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http://dx.doi.org/10.1007/s00604-021-04844-8DOI Listing
May 2021

Retraction notice to 'What we have learnt from the SARS epdemics in mainland China?' .

Glob Health J 2021 Mar 8;5(1):e1. Epub 2021 Apr 8.

Department of Infectious Disease Epidemiology, Institute of Microbiology and Epidemiology, Beijing 100850, China.

[This retracts the article DOI: 10.1016/j.glohj.2019.09.003.].
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http://dx.doi.org/10.1016/j.glohj.2020.12.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8112223PMC
March 2021

Lithium cobalt phosphate electrode for the simultaneous determination of ascorbic acid, dopamine, and serum uric acid by differential pulse voltammetry.

Mikrochim Acta 2021 May 15;188(6):190. Epub 2021 May 15.

Institute of Advanced Study, Chengdu University, No. 2025, Chengluo Avenue, Chengdu, People's Republic of China.

Lithium cobalt phosphate (LCP) was prepared and modified on the surface of glassy carbon electrode (GCE) to fabricate the electrochemical sensor (LCP/GCE) for the simultaneous determination of ascorbic acid (AA), dopamine (DA), and serum uric acid (UA). The homogenous incorporation of carbon improved the conductivity of LCP. Benefiting from the small particle size distribution, LCP/GCE has a large active surface and responds to AA, DA, and UA sensitively and rapidly. For the simultaneous detection with differential pulse voltammetry the anodic peaks of AA, DA, and UA were well-separated and appeared at ~0 V, ~0.19 V, and ~ 0.33 V (vs. Ag/AgCl), respectively. The linear responses toward AA, DA, and UA were in the range 10 μM-8.0 mM, 10 nM-10 μM, and 0.020 μM-25 μM; the detection limits were estimated to be 8.10 μM, 7.50 nM, and 22.7 nM (S/N = 3), respectively. The excellent selectivity and reproducibility of LCP/GCE enable serum UA to be detected without the interference of AA and DA. The recoveries of DA and AA in the serum sample were in the range 95 to 111%. The results indicate that LCP has the potential to be developed as the sensing devices to be applied to in vitro diagnosis. The lithium-ion battery cathodic material, LCP with the excellent adsorption and catalytic behavior, was utilized to fabricate the electrochemical sensor for the sensitive and simultaneous detection of AA, DA, and UA, which achieved the low detection limits and the wide concentration ranges. LCP/GCE can be used for the quantitative detection of serum UA without the interference of DA and AA. In addition, the recoveries of DA and AA in human serum were satisfactory, which illustrate the reliability of LCP/GCE to be applied to in vitro diagnosis.
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http://dx.doi.org/10.1007/s00604-021-04839-5DOI Listing
May 2021

A label-free fluorescent biosensor based on a catalyzed hairpin assembly for HIV DNA and lead detection.

Anal Methods 2021 Jun 11;13(21):2391-2395. Epub 2021 May 11.

College of Chemical Engineering, Sichuan University, Chengdu 610065, China.

Herein, a label-free fluorescent signal amplification system based on a catalyzed hairpin assembly (CHA) is reported. In this system, two hairpin probes, H1 and H2, were well-designed in which G-quadruplex sequences were integrated into H2. The CHA reaction was triggered by target/trigger DNA and G-quadruplex sequences were released, which can bind the fluorescent amyloid dye thioflavin T (ThT) to provide fluorescence signals. At the same time, target/trigger DNA was released from the product of the CHA reaction (H1-H2), which continued to initiate the next CHA cycle, and the signal was eventually amplified. This signal amplification approach has been successfully used to develop a label-free fluorescent sensing platform for sensitive detection of human immunodeficiency virus (HIV) DNA and Pb.
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http://dx.doi.org/10.1039/d1ay00410gDOI Listing
June 2021

Adherence and Efficacy of Smoking Cessation Treatment Among Patients with COPD in China.

Int J Chron Obstruct Pulmon Dis 2021 30;16:1203-1214. Epub 2021 Apr 30.

Tobacco Medicine and Tobacco Cessation Centre, Center of Respiratory Medicine, China-Japan Friendship Hospital, Beijing, People's Republic of China.

Background: Smoking cessation is a key intervention for all smokers with chronic obstructive pulmonary disease (COPD). Poor treatment adherence is a challenge in clinical practice that might contribute to the lower efficacy of medication (eg, oral drug). However, it is unclear what factors will influence adherence among smokers with COPD.

Methods: This study was based on an open-label randomized controlled trial (RCT) of varenicline and bupropion for smoking cessation among patients with COPD in China. The medication was given for 12 weeks, and visits and assessments were conducted at weeks 0, 1, 2, 4, 6, 9, 12, and 24. We assessed whether the adherence to smoking cessation treatment affects the smoking cessation efficacy and evaluated predictors of adherence.

Results: A total of 136 participants were recruited from February 2019 to June 2020, and analyzed using the intention-to-treat (ITT) method. In this study, 48.5% (66/136) of the total participants had good adherence to smoking cessation, and good adherence significantly improved the efficacy of smoking cessation (OR=9.60, 95% CI 4.02-22.96, P < 0.001). After adjusting for age, gender, nationality, education, and marital status, we found older age, higher education level, having more previous quitting attempts, stronger self-efficacy and preparation in quitting smoking, recognizing hazards of smoking, longer duration of COPD, and higher St. George's Respiratory Questionnaire (SGRQ) scores were relevant to good adherence (P < 0.05).

Conclusion: To our best knowledge, this is the first study to evaluate adherence to smoking cessation treatment among patients with COPD in China. Our study found that good adherence to smoking cessation treatment significantly improved the smoking cessation efficacy, and predictors of adherence were evaluated. We call on the medical community to pay attention to the adherence to smoking cessation among patients with COPD.
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http://dx.doi.org/10.2147/COPD.S301579DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096422PMC
April 2021

Trifunctional Electrolyte Additive Hexadecyltrioctylammonium Iodide for Lithium-Sulfur Batteries with Extended Cycle Life.

ACS Appl Mater Interfaces 2021 Apr 31;13(14):16545-16557. Epub 2021 Mar 31.

Institute of New Energy and Low-Carbon Technology (INELT), Sichuan University, Chengdu 610207, China.

Lithium-sulfur (Li-S) battery with a very high theoretical energy density (∼2500 Wh kg) is a very promising alternative to the commercial lithium-ion battery as the next-generation energy storage device. However, the Li-S battery suffers from shuttle effect and Li dendrites growth due to the solubility of polysulfides in the electrolyte system and the inhomogeneous deposition of Li, resulting in short cycling life span, which is the major obstacle in its practical application. Herein, we report an additive, hexadecyltrioctylammonium iodide (HTOA-I), in the conventional electrolyte system, which shows trifunctional effect on extending Li-S battery cycle life. It can not only help us to form a protective solid-electrolyte interface (SEI) on the surface of Li anode so as to reduce the contact of polysulfides with Li but also hinder the shuttling of polysulfides to the Li anode due to the strong combination of large-sized HTOA with polysulfide anions (S), which retard the migration of S and cause homogeneous Li deposition owing to the large size and stronger trend of HTOA to be absorbed on Li anode as well. A new method of phosphorescence analysis for direct observation of polysulfides shuttling has been put forward for the first time, which can be further developed in future studies. The cell with the HTOA-I-added electrolyte system shows high cycling stability, retaining 83.4% of the initial capacity after 200 cycles at 1 A g and achieving 689 mAh g even after 1000 cycles. This cost-effective and facile approach will not increase the complexity of the battery manufacturing process. Compared to other electrolyte additives, the additive in our work, HTOA-I, has better positive effects on extending cycle life. This trifunctional electrolyte additive will inspire the design of other new additives and further promote the development of Li-S batteries.
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http://dx.doi.org/10.1021/acsami.1c02580DOI Listing
April 2021

An integrated approach using ozone nanobubble and cyclodextrin inclusion complexation to enhance the removal of micropollutants.

Water Res 2021 May 11;196:117039. Epub 2021 Mar 11.

School of Environment, Northeast Normal University, 2555 Jingyue Street, Changchun 130117, China.

Ozone (O) has been widely used for the elimination of recalcitrant micropollutants in aqueous environments, due to its strong oxidation ability. However, the utilization efficiency of O is constrained by its low solubility and short half-life during the treatment process. Herein, an integrated approach, using nanobubble technology and micro-environmental chemistry within cyclodextrin inclusion cavities, was studied in order to enhance the reactivity of ozonisation. Compared with traditional macrobubble aeration with O in water, nanobubble aeration achieved 1.7 times higher solubility of O and increased the mass transfer coefficient 4.7 times. Moreover, the addition of hydroxypropyl-β-cyclodextrin (HPβCD) further increased the stability of O through formation of an inclusion complex in its molecule-specific cavity. At a HPβCD:O molar ratio of 10:1, the lifespan of O reached 18 times longer than in a HPβCD-free O solution. Such approach accelerated the removal efficiency of the model micropollutant, 4-chlorophenol by 6.9 times, compared with conventional macrobubble ozonation. Examination of the HPβCD inclusion complex by UV-visible spectroscopy and Nuclear Magnetic Resonance analyses revealed that both O and 4-chlorophenol entered the HPβCD cavity, and Benesi-Hildebrand plots indicated a 1:1 stoichiometry of the host and guest compounds. Additionally, molecular docking simulations were conducted in order to confirm the formation of a ternary complex of HPβCD:4-chlorophenol:O and to determine the optimal inclusion mode. With these results, our study highlights the viability of the proposed integrated approach to enhance the ozonation of organic micropollutants.
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http://dx.doi.org/10.1016/j.watres.2021.117039DOI Listing
May 2021

Knockdown of TMED3 inhibits cell viability and migration and increases apoptosis in human chordoma cells.

Int J Oncol 2021 05 24;58(5). Epub 2021 Mar 24.

Department of Spinal Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, P.R. China.

Chordoma is a rare low‑grade tumor of the axial skeleton. Over previous decades, a range of targeted drugs have been used for treating chordoma, with more specific and effective therapies under investigation. Transmembrane Emp24 protein transport domain containing 3 (TMED3) is a novel gene reported to be a regulator of oncogenesis, cancer development and metastasis; however, its role in chordoma remains unclear. In the present study, the expression of TMED3 was investigated in chordoma cells, and the effect of TMED3 knockdown on chordoma development was examined and , followed by exploration of differentially expressed proteins in TMED3‑silenced chordoma cells via an apoptosis antibody array. Reverse transcription‑quantitative PCR and western blot assays were performed to determine the expression levels. It was revealed that TMED3 was highly expressed in chordoma, and that knockdown of TMED3 inhibited cell viability and migration, and enhanced the apoptosis of chordoma cells. Additionally, knockdown of TMED3 inhibited the expression of Bcl‑2, heat shock protein 27, insulin‑like growth factor (IGF)‑I, IGF‑II, IGF binding protein‑2, Livin, Akt, CDK6 and cyclin D1 proteins, whereas MAPK9 was upregulated. Furthermore, a xenograft nude mice model demonstrated that TMED3 expression promoted tumor growth. Collectively, the present findings suggested that knockdown of TMED3 inhibited cell viability and migration, and enhanced apoptosis in chordoma cells, and that TMED3 may be a novel target for chordoma therapy.
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http://dx.doi.org/10.3892/ijo.2021.5195DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7949631PMC
May 2021

CRISPR-mediated rapid generation of neural cell-specific knockout mice facilitates research in neurophysiology and pathology.

Mol Ther Methods Clin Dev 2021 Mar 18;20:755-764. Epub 2021 Feb 18.

Department of Neurology, Thomas Jefferson University, Philadelphia, PA 19107, USA.

Inducible conditional knockout mice are important tools for studying gene function and disease therapy, but their generation is costly and time-consuming. We introduced clustered regularly interspaced short palindromic repeats (CRISPR) and into an LSL-Cas9 transgene-carrying mouse line by using adeno-associated virus (AAV)-PHP.eB to rapidly knockout gene(s) specifically in central nervous system (CNS) cells of adult mice. in neurons and in astrocytes were knocked out 2 weeks after an intravenous injection of vector, with an efficiency comparable to that of inducible Cre- conditional knockout. For functional testing, we generated astrocyte-specific knockout mice, which exhibited a phenotype similar to mice with Cre--mediated knockout, in an animal model of multiple sclerosis (MS), an autoimmune disorder of the CNS. With this novel technique, neural cell-specific knockout can be induced rapidly (few weeks) and cost-effectively. Our study provides a new approach to building inducible conditional knockout mice, which would greatly facilitate research on CNS biology and disease.
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http://dx.doi.org/10.1016/j.omtm.2021.02.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940702PMC
March 2021

Epidemiological analysis of asymptomatic SARS-CoV-2 transmission in the community: an individual-based model.

Sci Rep 2021 03 18;11(1):6251. Epub 2021 Mar 18.

China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, No. 119, South 4th Ring Road West, Fengtai District, Beijing, China.

We established an individual-based computer model to simulate the occurrence, infection, discovery, quarantine, and quarantine release (recovery) of asymptomatic SARS-CoV-2 infected individuals or patients within the community. The model was used to explore the effects of control measures, such as active tracing, laboratory testing, active treatment, and home quarantine on the epidemic. Considering the condition that R = 1.2, when a case of an imported asymptomatic infected individual (AII) was reported in the community, the implementation of control measures reduced the number of AIIs and patients by 62.2% and 62.4%, respectively. The number of undetected AIIs and patients peaked at 302 days of the epidemic, reaching 53 and 20 individuals, respectively. The implementation of sustained active tracing, laboratory testing, active treatment, and home quarantine can significantly reduce the probability of disease outbreaks and block the spread of the COVID-19 epidemic caused by AIIs in the community.
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http://dx.doi.org/10.1038/s41598-021-84893-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973571PMC
March 2021

Silicon nanoparticles-based ratiometric fluorescence platform: Real-time visual sensing to ciprofloxacin and Cu.

Spectrochim Acta A Mol Biomol Spectrosc 2021 May 18;253:119599. Epub 2021 Feb 18.

College of Chemistry, Sichuan University, Chengdu 610064, China; Key Laboratory of Green Chemistry and Technology, Ministry of Education, Sichuan University, Chengdu 610064, China.

In this work, a silicon nanoparticles (Si NPs)-based ratiometric fluorescence sensing platform was conveniently fabricated by simply mixing fluorescent Si NPs as co-ligands and reference signal with lanthanide metal ion Eu as response signal. The introduction of ciprofloxacin (CIP) remarkably turned on the characteristic fluorescence of Eu at 590 nm and 619 nm through the "antenna effect". At the same time, the blue emission of Si NPs at 445 nm kept comparatively stable. A good linear relationship between the ratio fluorescence intensity and CIP concentration in the range of 0.211-132.4 μM with a limit of detection (LOD) of 89 nM was obtained. In the presence of Cu, the fluorescence emission of Eu was sharply turned off because of the stronger coordination ability of Cu with CIP, which guaranteed the sequential detection of Cu. Meanwhile, the distinct fluorescent color change from bright blue to red, then back to blue, enabled naked-eye visual detection of CIP and Cu in the solution phase and on paper-based test strip, and was successfully applied to determine the levels of CIP in complicated food samples with high sensitivity.
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http://dx.doi.org/10.1016/j.saa.2021.119599DOI Listing
May 2021

T analysis using artificial neural networks.

J Magn Reson 2021 Apr 9;325:106930. Epub 2021 Feb 9.

Department of Physics, University of Windsor, 401 Sunset Avenue, Windsor, Canada. Electronic address:

Quantitative analysis of magnetic resonance signal lifetimes could reveal molecular scale information. However, it is non-trivial to recover the relaxation times from MR experiments in the multi-component exponential decay analysis. Constraints are required for the ill-posed problem in conventional inversion methods, which could lead to biased solutions. Artificial neural networks (ANNs) are a series of densely connected information processing nodes which cumulatively map a set of inputs to a set of outputs. They have proven to be universal approximators and powerful tools for solving complex nonlinear problems. In this work, ANNs were trained to recover T relaxation times. Both the discrete T spectrum and continuous T distribution were considered. Increased accuracy was achieved compared to the traditional methods. The continuous spectrum peak widths, generally not reliable in the traditional approach, could be determined accurately with ANN when the signal-to-noise ratio permitted.
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http://dx.doi.org/10.1016/j.jmr.2021.106930DOI Listing
April 2021

A gap existed between physicians' perceptions and performance of pain, agitation-sedation and delirium assessments in Chinese intensive care units.

BMC Anesthesiol 2021 Feb 25;21(1):61. Epub 2021 Feb 25.

Department of Critical Care Medicine, Beijing Tiantan Hospital, Capital Medical University, No. 119 South Fourth Ring West Road, Fengtai District, Beijing, 100070, China.

Background: Pain, agitation-sedation and delirium management are crucial elements in the care of critically ill patients. In the present study, we aimed to present the current practice of pain, agitation-sedation and delirium assessments in Chinese intensive care units (ICUs) and investigate the gap between physicians' perception and actual clinical performance.

Methods: We sent invitations to the 33 members of the Neuro-Critical Care Committee affiliated with the Chinese Association of Critical Care Physicians. Finally, 24 ICUs (14 general-, 5 neuroscience-, 3 surgical-, and 2 emergency-ICUs) from 20 hospitals participated in this one-day point prevalence study combined with an on-site questionnaire survey. We enrolled adult ICU admitted patients with a length of stay ≥24 h, who were divided into the brain-injured group or non-brain-injured group. The hospital records and nursing records during the 24-h period prior to enrollment were reviewed. Actual evaluations of pain, agitation-sedation and delirium were documented. We invited physicians on-duty during the 24 h prior to the patients' enrollment to complete a survey questionnaire, which contained attitude for importance of pain, agitation-sedation and delirium assessments.

Results: We enrolled 387 patients including 261 (67.4%) brain-injured and 126 (32.6%) non-brain-injured patients. There were 19.9% (95% confidence interval [CI]: 15.9-23.9%) and 25.6% (95% CI: 21.2-29.9%) patients receiving the pain and agitation-sedation scale assessment, respectively. The rates of these two types of assessments were significantly lower in brain-injured patients than non-brain-injured patients (p = 0.003 and < 0.001). Delirium assessment was only performed in three patients (0.8, 95% CI: 0.1-1.7%). In questionnaires collected from 91 physicians, 70.3% (95% CI: 60.8-79.9%) and 82.4% (95% CI: 74.4-90.4%) reported routine use of pain and agitation-sedation scale assessments, respectively. More than half of the physicians (52.7, 95% CI: 42.3-63.2%) reported daily screening for delirium using an assessment scale.

Conclusions: The actual prevalence of pain, agitation-sedation and delirium assessment, especially delirium screening, was suboptimal in Chinese ICUs. There is a gap between physicians' perceptions and actual clinical practice in pain, agitation-sedation and delirium assessments. Our results will prompt further quality improvement projects to optimize the practice of pain, agitation-sedation and delirium management in China.

Trial Registration: ClinicalTrials.gov, identifier NCT03975751 . Retrospectively registered on 2 June 2019.
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http://dx.doi.org/10.1186/s12871-021-01286-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905610PMC
February 2021

MARCH5 restores endothelial cell function against ischaemic/hypoxia injury via Akt/eNOS pathway.

J Cell Mol Med 2021 Apr 21;25(7):3182-3193. Epub 2021 Feb 21.

Department of Cardiology, West China Hospital, Sichuan University, Chengdu, China.

MARCH5 is a critical regulator of mitochondrial dynamics, apoptosis and mitophagy. However, its role in cardiovascular system remains poorly understood. This study aimed to investigate the role of MARCH5 in endothelial cell (ECs) injury and the involvement of the Akt/eNOS signalling pathway in this process. Rat models of myocardial infarction (MI) and human cardiac microvascular endothelial cells (HCMECs) exposed to hypoxia (1% O ) were used in this study. MARCH5 expression was significantly reduced in ECs of MI hearts and ECs exposed to hypoxia. Hypoxia inhibited the proliferation, migration and tube formation of ECs, and these effects were aggravated by knockdown of MARCH5 but antagonized by overexpressed MARCH5. Overexpression of MARCH5 increased nitric oxide (NO) content, p-eNOS and p-Akt, while MARCH5 knockdown exerted the opposite effects. The protective effects mediated by MARCH5 overexpression on ECs could be inhibited by eNOS inhibitor L-NAME and Akt inhibitor LY294002. In conclusion, these results indicated that MARCH5 acts as a protective factor in ischaemia/hypoxia-induced ECs injury partially through Akt/eNOS pathway.
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http://dx.doi.org/10.1111/jcmm.16386DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034466PMC
April 2021

Integration of Functionalized Polyelectrolytes onto Carbon Dots for Synergistically Improving the Tribological Properties of Polyethylene Glycol.

ACS Appl Mater Interfaces 2021 Feb 12;13(7):8794-8807. Epub 2021 Feb 12.

Institute for Advanced Study, Chengdu University, 2025 Chengluo Avenue, Chengdu 610106, P. R. China.

In this work, we carefully designed and synthesized a series of novel polyelectrolyte-functionalized carbon dots (CDs-PEI-X) by a facile and reversible phase transfer method based on the protonation reaction and anion exchange process executed on the surface of polyethylenimine-grafted CDs (CDs-PEI), where X denotes the anionic moieties of polyelectrolyte shells including hexafluorophosphate (PF), bis(trifluoromethane)sulfonimide (NTf), oleate (OL), and bis(salicylato)borate (BScB), respectively. Attributed to the favorable compatibility of these anions and polyethylene glycol (PEG) molecules, the hydrophobic CDs-PEI-X displayed excellent dispersibility and long-term stability in PEG200 base oil. Subsequently, the tribological behaviors of CDs-PEI-X as the lubricant additives of PEG200 were systematically investigated. It was proved that the anionic moieties of the polyelectrolyte shells of CDs-PEI-X played a crucial role in regulating their tribological behaviors. Particularly, CDs-PEI-OL was confirmed as an optimal additive, exhibiting the best lubricity, outstanding load-bearing capacity, long service life, and remarkable operational stability under boundary lubrication regime. Based on the tribological evaluations and worn surface analyses, the lubrication mechanism of CDs-PEI-OL was mainly attributed to the formation of the organic-inorganic hybrid adsorption film, the protective tribofilm, and its nanolubrication functions as scrollable "ball-bearing", i.e., the synergistic lubrication effects of surface polyelectrolyte shells and carbon cores. This study provides a feasible and versatile strategy to rapidly and effectively tailor the surface chemistry of CDs and discloses the essential contribution of carbon cores and surface groups on the lubrication process, which facilitates the development of advanced CDs-based nanolubricant additives.
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http://dx.doi.org/10.1021/acsami.0c22077DOI Listing
February 2021

A Trimetallic Cobalt/Iron/Nickel Phytate Catalyst for Overall Water Splitting: Fabrication by Magnetic-Field-Assisted Bipolar Electrodeposition.

Chempluschem 2021 Jan;86(1):184-190

College of Chemistry, Sichuan University, Chengdu, 610064, P. R. China.

Electrodeposition is an effective method to prepare various materials. We have established a bipolar electrodeposition system assisted by a constant magnetic field to fabricate a Co/Fe/Ni phytate catalyst with good electrocatalytic activity for overall water splitting. The effects of magnetic and electric fields on the catalytic properties of the material were studied. The catalyst prepared with an N-pole magnetic field (NPMF) exhibited good overall water splitting performance. Benefiting from the synergistic effect of the Co/Fe/Ni-phytate and the advantages of the N-pole magnetic field the NPMF electrode has a continuous 25 hours high-efficiency hydrogen evolution and oxygen evolution reaction at a current density of 100 mA cm in1.0 M KOH compared with commercial RuO and Pt/C. Bipolar electrodeposition with a constant magnetic field is thus an efficient means to fabricate electrocatalytic water splitting catalysts.
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http://dx.doi.org/10.1002/cplu.202000729DOI Listing
January 2021

Construction of the National Brain Tumor Registry of China for better management and more efficient use of data: a protocol.

BMJ Open 2021 01 17;11(1):e040055. Epub 2021 Jan 17.

Department of Neurosurgery, Beijing Tiantan Hospital, Beijing, China.

Introduction: Brain tumours encompass a complex group of intracranial tumours that mostly affect young adults and children, with a high incidence rate and poor prognosis. It remains impossible to systematically collect data on patients with brain tumours in China and difficult to perform in-depth analysis on the status of brain tumours, medical outcomes or other important medical issues through a multicentre clinical study. This study describes the first nation-wide data platform including the entire spectrum of brain tumour entities, which will allow better management and more efficient application of patient data in China.

Methods And Analysis: The National Brain Tumor Registry of China (NBTRC) is a registry of real-word clinical data on brain tumours. It is established and managed by the China National Clinical Research Center for Neurological Diseases and administered by its scientific and executive committees. The 54 participating hospitals of the NBTRC are located in 27 provinces/municipalities, performing more than 40 000 brain tumour surgeries per year. The data consist of in-hospital medical records, images and follow-up information after discharge. Data can be uploaded in three ways: the web portal, remote physical servers and offline software. The data quality control scheme is seven-dimensional. Each participating hospital could focus on a single pathology subtype and public subtypes of brain tumour for which they expect to conduct related multicentre clinical research. The standardised workflow to conduct clinical research is based on the benefit-sharing mechanism. Data collection will be conducted continuously from 1 February 2019 to 31 January 2024.

Ethics And Dissemination: Informed consent will be obtained from all participants. Consent for the adolescents' participation will be also obtained from their guardians via written consent. The results will be published in professional journals, in both Chinese and English.

Trial Registration Number: Chinese Clinical Trial Registry (ChiCTR1900021096).
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http://dx.doi.org/10.1136/bmjopen-2020-040055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813366PMC
January 2021

[Xiongcan Yishen Prescription upregulates the expressions of eNOS and cGMP in the penile tissue of ED rats with liver depression and kidney deficiency].

Zhonghua Nan Ke Xue 2020 Feb;26(2):167-173

Department of Andrology, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Human 410007, China.

Objective: To investigate the effect of Xiongcan Yishen Prescription (XYP) on the expressions of eNOS and cGMP in the penile tissue of ED rats with liver depression and kidney deficiency (LDKD).

Methods: The model of ED-LDKD was established in 30 eight-week-old SPF-class male SD rats by injecting hydrocortisone intramuscularly and binding the limbs for 14 days, and another 10 rats were taken as blank controls. Then, the model rats were randomized into six groups of equal number and treated intragastrically with distilled water (model control), tadalafil tablets at 0.52 mg/kg/d (tadalafil control), Shugan Yiyang Capsules 0.3125 g/kg/d (SYC control), and XYP at 10.4 g/kg/d (low-dose XYP), 20.8 g/kg/d (medium-dose XYP) and 41.6 g/kg/d (high-dose XYP), bid, for 28 successive days, respectively. Before and after modeling and after 28-day treatment, the animals were subjected to tail suspension and mating tests. The next day after medication, the penile tissues of the rats were harvested for determining the expression levels of eNOS and cGMP proteins by immunohistochemical analysis of the mean optical density.

Results: Compared with the model controls, the rats of the high-, medium- and low-dose XYP and SYC control groups all showed significant decreases in the tail suspension time ([3.17 ± 0.11] vs [2.58 ± 0.25], [2.52 ± 0.31], [2.51 ± 0.3] and [2.57 ± 0.29] min, P < 0.05) and mount latency (ML) ([9.23 ± 0.11] vs [1.21 ± 0.12], [2.17 ± 0.16], [2.26 ± 0.13], [1.23 ± 0.15] and [2.48 ± 0.18] min, P < 0.05) but increases in mount frequency (MF) ([0.48 ± 0.18] vs [3.29 ± 0.11], [3.18 ± 0.11], [3.05 ± 0.05], [3.23 ± 0.12] and [3.2 ± 0.28] times, P < 0.05) and intromission frequency (IF) ([0.8 ± 0.84] vs [11.8 ± 0.84], [11.2 ± 1.48], [9.4 ± 1.14], [11.4 ± 1.14] and [10 ± 1.22] times, P < 0.05). The eNOS and cGMP proteins were mainly expressed in the nucleus and cytoplasm of the arterial and venous endothelial cells and sinusoidal endothelial cells of the cavernous, as brownish yellow particles in a scattered and focal pattern. Both the expressions of eNOS and cGMP in the penile tissue were remarkably upregulated in the high-, medium- and low-dose XYP and SYC control groups as compared with those in the model control (P < 0.05) but exhibited no statistically significant difference between the tadalafil and model control groups (P > 0.05).

Conclusions: Xiongcan Yishen Prescription can relieve the depression symptoms, increase the mount frequency, activate the NO/cGMP pathway, and upregulate the expressions of eNOS and cGMP in the penile tissue of ED rats with liver depression and kidney deficiency.
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February 2020

Analysis and prediction of the coronavirus disease epidemic in China based on an individual-based model.

Sci Rep 2020 12 17;10(1):22123. Epub 2020 Dec 17.

China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, No. 119, South 4th Ring Road West, Fengtai District, Beijing, China.

We established a stochastic individual-based model and simulated the whole process of occurrence, development, and control of the coronavirus disease epidemic and the infectors and patients leaving Hubei Province before the traffic was closed in China. Additionally, the basic reproduction number (R) and number of infectors and patients who left Hubei were estimated using the coordinate descent algorithm. The median R at the initial stage of the epidemic was 4.97 (95% confidence interval [CI] 4.82-5.17). Before the traffic lockdown was implemented in Hubei, 2000 (95% CI 1982-2030) infectors and patients had left Hubei and traveled throughout the country. The model estimated that if the government had taken prevention and control measures 1 day later, the cumulative number of laboratory-confirmed patients in the whole country would have increased by 32.1%. If the lockdown of Hubei was imposed 1 day in advance, the cumulative number of laboratory-confirmed patients in other provinces would have decreased by 7.7%. The stochastic model could fit the officially issued data well and simulate the evolution process of the epidemic. The intervention measurements nationwide have effectively curbed the human-to-human transmission of severe acute respiratory syndrome coronavirus 2.
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http://dx.doi.org/10.1038/s41598-020-76969-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7747602PMC
December 2020

Loading MicroRNA-376c in Extracellular Vesicles Inhibits Properties of Non-Small Cell Lung Cancer Cells by Targeting YTHDF1.

Technol Cancer Res Treat 2020 Jan-Dec;19:1533033820977525

Department of Thoracic Oncology, Jiangxi Cancer Hospital, Nanchang, Jiangxi, People's Republic of China.

Objective: Extracellular vesicles (Evs) secreted from cells have been revealed to mediate signal transduction between cells. Nevertheless, the mechanisms through which molecules transported by EVs function remain to be elucidated. In the present study, the functional relevance of endothelial cells (ECs)-secreted Evs carrying microRNA-376c (miR-376c) in the biological activities of non-small cell lung cancer (NSCLC) cells was investigated, including the related mechanisms.

Methods: Two cell lines with the highest YTH N6-methyladenosine (m6A) RNA binding protein 1 (YTHDF1) expression were selected for subsequent experiments. Cellular proliferation, migration, invasion and apoptosis were measured by EdU, wound healing, Transwell assays and flow cytometry, respectively. The binding relationship between miR-376c and YTHDF1 was analyzed by dual-luciferase reporter assays. The miR-376c, YTHDF1 and β-catenin expression was evaluated by qPCR assays and western blot assays.

Results: The expression patterns of YTHDF1 were higher in NSCLC cells, whereas miR-376c was reduced versus the normal bronchial epithelial cells. Silencing of YTHDF1 repressed NSCLC cell proliferation, invasion and migration abilities, whereas enhanced apoptosis. miR-376c negatively modulated YTHDF1 expression. Under co-culture conditions, ECs transmitted miR-376c into NSCLC cells through Evs, and inhibited the intracellular YTHDF1 expression and the Wnt/β-catenin pathway activation. Rescue experiments revealed that YTHDF1 overexpression reversed the inhibitory role of miR-376c released by EC-Evs in NSCLC cells.

Conclusion: EC-delivered Evs inhibit YTHDF1 expression and the Wnt/β-catenin pathway induction via miR-376c overexpression, thus inhibiting the malignant phenotypes of NSCLC cells.
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http://dx.doi.org/10.1177/1533033820977525DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7724269PMC
December 2020

Ambient Pressure Ion Funnel: Concepts, Simulations, and Analytical Performance.

Anal Chem 2020 12 1;92(24):15811-15817. Epub 2020 Dec 1.

School of Chemistry, University of New South Wales, Sydney, NSW 2052 Australia.

In mass spectrometry (MS), a major loss of ions can readily occur during their transfer from atmospheric pressure to a lower pressure, which limits performance. Here, we report an ion funnel that can be used to effectively focus ions at ambient pressure (∼777 Torr) to significantly enhance performance in electrospray ionization (ESI) MS. For seven singly charged test ions (/ 124-1131), the ambient pressure ion funnel (APIF) is demonstrated to improve ion abundances, sensitivity, and detection limits by up to factors of ∼17, ∼16, and ∼3, respectively, compared to the operation of conventional ESI-MS. Simulated ion trajectories were used to rationalize the enhanced performance of the APIF, which is attributed primarily to using a relatively high RF field amplitude to radially confine ions, a high DC field, and a wide exit ring electrode. The effective focusing of ions at ambient pressures should be beneficial in the future for improving the performance of (i) additional methods that ionize molecules at atmospheric pressure, (ii) ambient pressure ion mobility-based instruments, and (iii) high flow rate liquid chromatography mass spectrometry platforms.
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http://dx.doi.org/10.1021/acs.analchem.0c02938DOI Listing
December 2020

Interferon-γ/Interleukin-27 Axis Induces Programmed Death Ligand 1 Expression in Monocyte-Derived Dendritic Cells and Restores Immune Tolerance in Central Nervous System Autoimmunity.

Front Immunol 2020 26;11:576752. Epub 2020 Oct 26.

Department of Neurology, Thomas Jefferson University, Philadelphia, PA, United States.

Antigen (Ag)-specific tolerance induction by intravenous (i. v.) injection of high-dose auto-Ags has been explored for therapy of autoimmune diseases, including multiple sclerosis (MS). It is thought that the advantage of such Ag-specific therapy over non-specific immunomodulatory treatments would be selective suppression of a pathogenic immune response without impairing systemic immunity, thus avoiding adverse effects of immunosuppression. Auto-Ag i.v. tolerance induction has been extensively studied in experimental autoimmune encephalomyelitis (EAE), an animal model of MS, and limited clinical trials demonstrated that it is safe and beneficial to a subset of MS patients. Nonetheless, the mechanisms of i.v. tolerance induction are incompletely understood, hampering the development of better approaches and their clinical application. Here, we describe a pathway whereby auto-Ag i.v. injected into mice with ongoing clinical EAE induces interferon-gamma (IFN-γ) secretion by auto-Ag-specific CD4 T cells, triggering interleukin (IL)-27 production by conventional dendritic cells type 1 (cDC1). IL-27 then, signal transducer and activator of transcription 3 activation, induces programmed death ligand 1 (PD-L1) expression by monocyte-derived dendritic cells (moDCs) in the central nervous system of mice with EAE. PD-L1 interaction with programmed cell death protein 1 on pathogenic CD4 T cells leads to their apoptosis/anergy, resulting in disease amelioration. These findings identify a key role of the IFN-γ/IL-27/PD-L1 axis, involving T cells/cDC1/moDCs in the induction of i.v. tolerance.
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http://dx.doi.org/10.3389/fimmu.2020.576752DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7649367PMC
October 2020

MicroRNA-103a Curtails the Stemness of Non-Small Cell Lung Cancer Cells by Binding OTUB1 via the Hippo Signaling Pathway.

Technol Cancer Res Treat 2020 Jan-Dec;19:1533033820971643

Department of Thoracic Oncology Ward 2, 146391Jiangxi Tumor Hospital, Nanchang, Jiangxi, People's Republic of China.

Objective: Although microRNA-103a (miR-103a) dysfunction has been implicated in various cancers, its relevance to non-small cell lung cancer (NSCLC) has not been clarified. This study was conducted to examine the molecular mechanism underlying the regulatory role of miR-103a in NSCLC.

Methods: Kaplan-Meier analysis was carried out to assess the relationship between overall survival of NSCLC patients and miR-103a expression. Reverse-transcription quantitative polymerase chain reaction and western blot analyses were applied to evaluate the expression of relevant genes in tissues and cells. Sphere formation, MTS, flow cytometry, and Transwell assays were performed to characterize stemness. Dual luciferase reporter gene assays were used to clarify the binding relationship between miR-103a and ovarian tumor domain-containing ubiquitin aldehyde binding protein 1 (OTUB1). Finally, western blot analysis was used to assess the involvement of the Hippo pathway in NSCLC.

Results: In NSCLC tissues and cells, miR-103a was expressed at low levels, whereas OTUB1 was expressed at high levels. Higher miR-103 expression levels were associated with a better prognosis for patients with NSCLC. When miR-103a was overexpressed, cell viability and stemness decreased, whereas apoptosis and cell cycle arrest were facilitated. The expression of phosphorylated YAP also decreased significantly. Opposite trends were observed after miR-103a silencing. OTUB1 expression and YAP phosphorylation decreased in the presence of miR-103a, and OTUB1 overexpression blocked the inhibitory effects of miR-103a on NSCLC cells.

Conclusion: The miR-103a/OTUB1/Hippo axis may play a role in modulating the malignant behavior and stemness of cancer stem cells and thus could be a potential therapeutic target for the management of NSCLC.
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http://dx.doi.org/10.1177/1533033820971643DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672774PMC
November 2020

Oligodendrocyte-derived extracellular vesicles as antigen-specific therapy for autoimmune neuroinflammation in mice.

Sci Transl Med 2020 11;12(568)

Department of Neurology, Thomas Jefferson University, Philadelphia, PA 19107, USA.

Autoimmune diseases such as multiple sclerosis (MS) develop because of failed peripheral immune tolerance for a specific self-antigen (Ag). Numerous approaches for Ag-specific suppression of autoimmune neuroinflammation have been proven effective in experimental autoimmune encephalomyelitis (EAE), an animal model of MS. One such approach is intravenous tolerance induction by injecting a myelin Ag used for triggering EAE. However, the translation of this and similar experimental strategies into therapy for MS has been hampered by uncertainty regarding relevant myelin Ags in MS patients. To address this issue, we developed a therapeutic strategy that relies on oligodendrocyte (Ol)-derived extracellular vesicles (Ol-EVs), which naturally contain multiple myelin Ags. Intravenous Ol-EV injection reduced disease pathophysiology in a myelin Ag-dependent manner, both prophylactically and therapeutically, in several EAE models. The treatment was safe and restored immune tolerance by inducing immunosuppressive monocytes and apoptosis of autoreactive CD4 T cells. Furthermore, we showed that human Ols also released EVs containing most relevant myelin Ags, providing a basis for their use in MS therapy. These findings introduce an approach for suppressing central nervous system (CNS) autoimmunity in a myelin Ag-specific manner, without the need to identify the target Ag.
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http://dx.doi.org/10.1126/scitranslmed.aba0599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886371PMC
November 2020

A distinct GM-CSF T helper cell subset requires T-bet to adopt a T1 phenotype and promote neuroinflammation.

Sci Immunol 2020 10;5(52)

Department of Neurology, Thomas Jefferson University, Philadelphia, PA, USA.

Elevation of granulocyte-macrophage colony-stimulating factor (GM-CSF)-producing T helper (T) cells has been associated with several autoimmune diseases, suggesting a potential role in the pathogenesis of autoimmunity. However, the identity of GM-CSF-producing T cells has not been closely examined. Using single-cell RNA sequencing and high-dimensional single-cell mass cytometry, we identified eight populations of antigen-experienced CD45RACD4 T cells in blood of healthy individuals including a population of GM-CSF-producing cells, known as TGM, that lacked expression of signature transcription factors and cytokines of established T lineages. Using GM-CSF-reporter/fate reporter mice, we show that TGM cells are present in the periphery and central nervous system in a mouse model of experimental autoimmune encephalomyelitis. In addition to GM-CSF, human and mouse TGM cells also expressed IL-2, tumor necrosis factor (TNF), IL-3, and CCL20. TGM cells maintained their phenotype through several cycles of activation but up-regulated expression of T-bet and interferon-γ (IFN-γ) upon exposure to IL-12 in vitro and in the central nervous system of mice with autoimmune neuroinflammation. Although T-bet was not required for the development of TGM cells, it was essential for their encephalitogenicity. These findings demonstrate that TGM cells constitute a distinct population of T cells with lineage characteristics that are poised to adopt a T1 phenotype and promote neuroinflammation.
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http://dx.doi.org/10.1126/sciimmunol.aba9953DOI Listing
October 2020

miR-150 regulates glucose utilization through targeting GLUT4 in insulin-resistant cardiomyocytes.

Acta Biochim Biophys Sin (Shanghai) 2020 Oct;52(10):1111-1119

Department of Pharmacology, Harbin Medical University (The State-Province Key Laboratories of Biomedicine-Pharmaceutics of China), Harbin 150081, China.

MicroRNAs (miRNAs) play an important role in cardiac function and metabolism. However, whether they regulate insulin resistance (IR) of cardiomyocytes remains unclear. The aim of the present study was to shed light on this issue with a focus on miR-150. We found here that miR-150 level was elevated in myocardium of type 2 diabetes mellitus (T2DM) rat model and in insulin-resistant cardiomyocytes induced by high glucose (25 mM) and high insulin (1 μM). Deregulation of miR-150 downregulated the protein and mRNA levels of glucose transporter 4 (GLUT4) as assessed by western blot, real-time polymerase chain reaction (qPCR), and immunofluorescence assays. Overexpression of miR-150 inhibited glucose utilization in cardiomyocytes as detected by 2-deoxyglucose transport and glucose consumption assays. In contrast, knockdown of miR-150 significantly increased glucose uptake in cardiomyocytes. Moreover, GLUT4 translocation was increased after transfection of miR-150 inhibitor (AMO-150). Collectively, miR-150 reduced glucose utilization by directly decreasing the expression and translocation of GLUT4 in the cardiomyocytes with IR and therefore might be a new therapeutic target for metabolic diseases such as T2DM.
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http://dx.doi.org/10.1093/abbs/gmaa094DOI Listing
October 2020

Rapid and colorimetric detection of nucleic acids based on entropy-driven circuit and DNAzyme-mediated autocatalytic reactions.

Anal Methods 2020 06;12(21):2779-2784

College of Chemistry, Sichuan University, Chengdu 610064, China.

In this work, a novel, rapid and enzyme-free colorimetric biosensor for the detection of nucleic acids has been developed based on entropy-driven (EDC) circuit and DNAzyme-mediated autocatalytic reactions. On sensing the target DNA, the EDC reaction could be initiated and the intact Mg2+-dependent DNAzyme was formed in the reaction product; then, a "mimic target" DNA was generated during the cleavage process of DNAzyme, which in turn catalyzed the EDC reaction corresponding to an autocatalytic process. Meanwhile, numerous G-quadruplex structures were released and further interacted with hemin to form peroxidase-mimicking DNAzyme, inducing a remarkably amplified colorimetric signal. This autocatalytic EDC (AEDC) sensing system exhibited a linear relationship in the range from 20 pM to 10 nM with a detection limit of 10.2 pM. More importantly, benefitting from the Mg2+-dependent DNAzyme-mediated autocatalytic reaction, the detection time (20 min) was significantly reduced compared to that for the reported EDC strategies. In addition, this sensing system has been applied for the detection of target DNA in human serum samples, indicating that it is promising for the on-site and real-time detection of nucleic acids in biomedical research and disease diagnosis.
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http://dx.doi.org/10.1039/d0ay00341gDOI Listing
June 2020