Publications by authors named "Chi Yang"

403 Publications

Facile synthesis of axially chiral styrene-type carboxylic acids palladium-catalyzed asymmetric C-H activation.

Chem Sci 2021 Jan 20;12(10):3726-3732. Epub 2021 Jan 20.

Hefei National Laboratory for Physical Sciences at the Microscale, Department of Chemistry, Center for Excellence in Molecular Synthesis of CAS, University of Science and Technology of China Hefei Anhui 230026 China

A novel method by a one-step introduction of axial chirality and sterically hindered group has been developed for facile synthesis of axially chiral styrene-type carboxylic acids. With the palladium-catalyzed C-H arylation and olefination of readily available cinnamic acid established, this transformation demonstrated excellent yield, excellent stereocontrol (up to 99% yield and 99% ee), and broad substrate scope under mild conditions. The axially chiral styrene-type carboxylic acids produced have been successfully applied to Cp*Co-catalyzed asymmetric C-H activation reactions, indicating their potential as chiral ligands or catalysts in asymmetric synthesis.
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http://dx.doi.org/10.1039/d0sc06661cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179534PMC
January 2021

Dencichine prevents ovariectomy-induced bone loss and inhibits osteoclastogenesis by inhibiting RANKL-associated NF-κB and MAPK signaling pathways.

J Pharmacol Sci 2021 Aug 3;146(4):206-215. Epub 2021 May 3.

Department of Orthopaedics, Yancheng City No.1 People's Hospital, Yancheng, Jiangsu 224006, China. Electronic address:

Aims: To investigate the effect of dencichine on osteoclastogenesis in vivo and in vitro.

Methods: RANKL-induced osteoclastogenesis were treated with different concentrations of dencichine. Pit forming assays were applied to evaluate the degree of bone resorption. Osteoclastogenic markers were detected by real-time quantitative PCR (RT-qPCR) and Western blot. Micro CT was conducted to investigate the effects of dencichine on osteoclastogenesis in ovariectomized (OVX) mice.

Results: Dencichine suppressed osteoclastogenesis through the inhibition of phosphorylation of p65, p50 (NF-κB pathway), p38, ERK and JNK (MAPKs pathway) in vitro. Furthermore, dencichine inhibited the function of osteoclasts in a dose-dependent manner. In addition, the expression levels of the nuclear factor of activated T cells 1 (NFATc1) and osteoclastogenesis markers were decreased by dencichine, including MMP-9, Cathepsin K (CTSK), Tartrate-Resistant Acid Phosphatase (TRAP), C-FOS, dendritic cell specific transmembrane protein (DC-STAMP). In vivo data proved that dencichine alleviated ovariectomy-induced bone loss and osteoclastogenesis in mice.

Conclusion: Our results demonstrate that dencichine alleviates OVX-induced bone loss in mice and inhibits RANKL-mediated osteoclastogenesis via inhibition of NF-κB and MAPK pathways in vitro, suggesting that dencichine might serve as a promising candidate for treatment of bone loss diseases, including PMOP and rheumatoid arthritis.
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http://dx.doi.org/10.1016/j.jphs.2021.04.004DOI Listing
August 2021

Au-to-Ag coordinate-covalent bonding and other supramolecular interactions with covalent bonding strength.

Chem Sci 2020 Sep 11;11(41):11179-11188. Epub 2020 Sep 11.

Department of Chemistry, University of North Texas 1155 Union Circle #305070 Denton Texas 76203 USA

An efficient strategy for designing charge-transfer complexes using coinage metal cyclic trinuclear complexes (CTCs) is described herein. Due to opposite quadrupolar electrostatic contributions from metal ions and ligand substituents, [Au(μ-Pz-(i-CH))]·[Ag(μ-Tz-(-CF))] (Pz = pyrazolate, Tz = triazolate) has been obtained and its structure verified by single crystal X-ray diffraction - representing the 1 crystallographically-verified stacked adduct of monovalent coinage metal CTCs. Abundant supramolecular interactions with aggregate covalent bonding strength arise from a combination of M-M' (Au → Ag), metal-π, π-π interactions and hydrogen bonding in this charge-transfer complex, according to density functional theory analyses, yielding a computed binding energy of 66 kcal mol between the two trimer moieties - a large value for intermolecular interactions between adjacent d centres (nearly doubling the value for a recently-claimed Au(i) → Cu(i) polar-covalent bond: , 2017, , E5042) - which becomes 87 kcal mol with benzene stacking. Surprisingly, DFT analysis suggests that: (a) some other literature precedents should have attained a stacked product akin to the one herein, with similar or even higher binding energy; and (b) a high overall intertrimer bonding energy by inferior electrostatic assistance, underscoring genuine orbital overlap between M and M' frontier molecular orbitals in such polar-covalent M-M' bonds in this family of molecules. The Au → Ag bonding is reminiscent of classical Werner-type coordinate-covalent bonds such as HN: → Ag in [Ag(NH)], as demonstrated herein quantitatively. Solid-state and molecular modeling illustrate electron flow from the π-basic gold trimer to the π-acidic silver trimer with augmented contributions from ligand-to-ligand' (LL'CT) and metal-to-ligand (MLCT) charge transfer.
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http://dx.doi.org/10.1039/d0sc02520hDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162903PMC
September 2020

Salicylic acid-based nanomedicine with self-immunomodulatory activity facilitates microRNA therapy for metabolic skeletal disorders.

Acta Biomater 2021 Jun 3. Epub 2021 Jun 3.

National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Department of Oral Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China. Electronic address:

Metabolic skeletal disorders remain a major clinical challenge. The complexity of this disease requires a strategy to address the net effects of both inflammation and impaired bone formation. microRNA-based gene therapy provides several therapeutic advantages to tackle these issues. Herein, we describe a microRNA-21 (miR-21) delivery system with an additional therapeutic effect from that of the delivery carrier itself. Poly (salicylic acid) (PSA) is, for the first time, synthesized via polycondensation of salicylic acid (SA), a bioactive ingredient widely used for anti-inflammation in medicine. PSA can self-assemble into nanoparticles (PSA-NPs) and can effectively deliver genes both in vitro and in vivo. The carrier was then attached to repetitive sequences of aspartate, serine, serine (DSS) for delivering miRNAs specifically to bone-formation surfaces. In vitro studies showed that [email protected] could effectively realize the intracellular delivery of miR-21 with low toxicity, while in vivo results indicated that the [email protected] prolonged blood circulation time, enhanced bone accumulation, and significantly improved the efficacy of miR-21-based bone anabolic therapy in osteoporotic mice. The constructed delivery system ([email protected]) inherited the advantages of both SA and miR-21, which could ameliorate bone-inflamed niche and rescued the impaired bone formation ability. The synergy of anti-inflammatory and pro-osteogenic effects significantly improved trabecular bone microstructure in osteoporotic mice. STATEMENT OF SIGNIFICANCE: The complexity of metabolic skeletal disorders requires a strategy to address the net effects of both inflammation and impaired bone formation. microRNA-based gene therapy provides several therapeutic advantages to tackle these issues. We develop a novel microRNA-21 delivery system with additional therapeutic effect from that of the gene carrier itself. Poly (salicylic acid) (PSA) nanoparticles, for the first time, synthesized via polycondensation of salicylic acid and can effectively deliver genes both in vitro and in vivo. The constructed delivery system ([email protected]) inherited the advantages of both SA (commonly used anti-inflammation drug in medicine) and miR-21 (a pro-osteogenic molecule), which could ameliorate bone-inflamed niche, rescued impaired bone formation ability and significantly improved trabecular bone microstructure in osteoporotic mice.
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http://dx.doi.org/10.1016/j.actbio.2021.05.024DOI Listing
June 2021

Chromosome-scale assembly of the Sparassis latifolia genome obtained using long-read and Hi-C sequencing.

G3 (Bethesda) 2021 May 21. Epub 2021 May 21.

Institute of Edible Mushroom, Fujian Academy of Agricultural Sciences, Fuzhou 350014, China; National and Local Joint Engineering Research Center for Breeding & Cultivation of Featured Edible Mushroom, Fujian Academy of Agricultural Sciences, Fuzhou 350014, China.

Sparassis latifolia is a valuable edible mushroom cultivated in China. In 2018, our research group reported an incomplete and low-quality genome of S. latifolia obtained by Illumina HiSeq 2500 sequencing. These limitations in the available genome have constrained genetic and genomic studies in this mushroom resource. Herein, an updated draft genome sequence of S. latifolia was generated by Oxford Nanopore sequencing and the Hi-C technique. A total of 8.24 Gb of Oxford Nanopore long reads representing ∼198.08X coverage of the S. latifolia genome were generated. Subsequently, a high-quality genome of 41.41 Mb, with scaffold and contig N50 sizes of 3.31 Mb and 1.51 Mb, respectively, was assembled. Hi-C scaffolding of the genome resulted in 12 pseudochromosomes containing 93.56% of the bases in the assembled genome. Genome annotation further revealed that 17.47% of the genome was composed of repetitive sequences. In addition, 13,103 protein-coding genes were predicted, among which 98.72% were functionally annotated. BUSCO assay results further revealed that there were 92.07% complete BUSCOs. The improved chromosome-scale assembly and genome features described here will aid further molecular elucidation of various traits, breeding of S. latifolia, and evolutionary studies with related taxa.
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http://dx.doi.org/10.1093/g3journal/jkab173DOI Listing
May 2021

Hamstrings load bearing in different contraction types and intensities: A shear-wave and B-mode ultrasonographic study.

PLoS One 2021 19;16(5):e0251939. Epub 2021 May 19.

Faculty of Sport Sciences, Waseda University, Tokyo, Japan.

The main aim was to examine the load bearing of individual hamstring muscles in different contraction types and intensities, through local stiffness measurement by shear wave elastography (SWE). A secondary aim was to examine the relationship between the SWE stiffness measure and hamstrings morphology. Ten healthy males (age 22.1±4.1 years; height 173.7±5.9 cm; body mass 68.6±12.4 kg; mean ± SD) performed knee flexions on an isokinetic dynamometer at different intensities (20-70%MVC, random order) in three separate, randomized conditions: isometric (ISO), concentric (CON) and eccentric (ECC). SWE was used to measure muscle shear wave velocity (SWV) in biceps femoris long head (BFlh), semitendinosus (ST) and semimembranosus (SM) during contraction. Muscle anatomical cross-sectional area (ACSA) was measured with magnetic resonance imaging and muscle architecture with B-mode ultrasonography. Muscle SWV increased linearly with contraction intensity, but at a varying rate among muscles and contraction types. ST exhibited greater SWV than BFlh and SM in all contraction types, however, there was an upward shift in the SM SWV-torque relationship in ECC compared to ISO and CON. Strong negative correlations were found between peak ISO SWV and ST ACSA (r = -0.81, p = 0.005) and BFlh pennation angle (r = -0.75, p = 0.012). These results suggest that ST has a primary role in hamstrings load bearing in all contraction types, likely due to its morphology; however, there is evidence of increased contribution from SM in eccentric muscle actions.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0251939PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133428PMC
May 2021

Copper-catalyzed monochloromethylazidation to access transformable terminal alkyl chlorides using stoichiometric BrCHCl.

Chem Commun (Camb) 2021 Jun;57(46):5666-5669

Hefei National Laboratory for Physical Sciences at the Microscale and Department of Chemistry, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230026, China.

Efficient copper-catalyzed 1,2-difunctionalization of alkenes with commercially available BrCH2Cl as a chloromethylating source was carried out, in which mild conditions, high reactivity, excellent functional-group tolerance, and late-stage modification of a bioactive molecule are demonstrated. This strategy offers a solution for the diverse syntheses of nitrogen-containing terminal alkyl chlorides, a common synthetic handle that is promising for multiple derivatizations. Mechanistic studies indicate that a chloromethyl radical is involved in the catalytic cycle.
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http://dx.doi.org/10.1039/d1cc01751aDOI Listing
June 2021

Comparison of Osteogenic Differentiation Potential of Human Dental-Derived Stem Cells Isolated from Dental Pulp, Periodontal Ligament, Dental Follicle, and Alveolar Bone.

Stem Cells Int 2021 7;2021:6631905. Epub 2021 Apr 7.

Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, Liaoning, China.

Background: Mesenchymal stem cells (MSCs) have become promising candidates for regeneration medicine due to their multidifferentiation potential and immunomodulatory ability. Compared with classic MSCs derived from the bone marrow and fat, dental-derived MSCs show high plasticity, accessibility, and applicability. Therefore, they are considered alternative sources for regeneration medicine.

Methods: Four types of MSCs were isolated from the dental pulp, periodontal ligament, dental follicle, and alveolar bone of the same donor, and there were five different individuals. We analyzed their morphology, immunophenotype, proliferation rate, apoptosis, trilineage differentiation potential, and the gene expression during osteogenic differentiation.

Results: Our research demonstrated that DPSCs, PDLSCs, DFPCs and ABMMSCs exhibited similar morphology and immunophenotype. DFPCs showed a higher rate of proliferation and apoptosis. When cultured in the trilineage differentiation medium, all types of MSCs presented the differentiation potential of osteogenesis, adipogenesis, and chondrogenesis. Through staining and genetic analysis during osteogenic induction, ABMMSCs and PDLSCs showed the highest osteogenic ability, followed by DPSCs, and DFPCs were the lowest.

Conclusions: Overall, our results indicated that different dental-derived stem cells possessed different biological characteristics. For bone tissue engineering, ABMMSCs and PDLSCs can be used as optimal candidates of seed cells.
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http://dx.doi.org/10.1155/2021/6631905DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049831PMC
April 2021

Pathologies associated with the mandibular third molar impaction.

Sci Prog 2021 Apr-Jun;104(2):368504211013247

Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

To evaluate the associations of impaction patterns of mandibular third molars (M3Ms) with pathologies caused by them. In this study, 262 patients with 432 impacted M3Ms who referred in Shanghai Xuhui District Center were reviewed. The pathologies include pericoronitis, mandibular second molar (M2M) caries, and M2M distal periodontal pathology. The impaction patterns of M3Ms and the pathologies were examined, while the M2M outcomes after surgeries were evaluated. A χ test was used to analyze the data, with a value of <0.05 being considered statistically significant. Pericoronitis was the major symptom in all patients, whereas the propensities of M2M distal caries and periodontal pathologies increased in older patients. Soft tissue impacted and vertically angulated teeth were more associated with pericoronitis ( < 0.05); mesio-angular impacted teeth in less deep positions had greater risks of M2Ms distal caries ( < 0.05); mesio-angular and horizontal impacted teeth in relative deep positions were more likely to cause M2Ms distal periodontal pathologies ( < 0.05). Extractions of soft tissue impacted teeth in vertical angulations should be considered, while removals of mesially and horizontally angulated or bony impacted teeth could be delayed.
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http://dx.doi.org/10.1177/00368504211013247DOI Listing
April 2021

Direct Imaging of Integrated Circuits in CPU with 60 nm Super-Resolution Optical Microscope.

Nano Lett 2021 05 27;21(9):3887-3893. Epub 2021 Apr 27.

Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.

Far-field super-resolution optical microscopies have achieved incredible success in life science for visualization of vital nanostructures organized in single cells. However, such resolution power has been much less extended to material science for inspection of human-made ultrafine nanostructures, simply because the current super-resolution optical microscopies modalities are rarely applicable to nonfluorescent samples or unlabeled systems. Here, we report an antiphase demodulation pump-probe (DPP) super-resolution microscope for direct optical inspection of integrated circuits (ICs) with a lateral resolution down to 60 nm. Because of the strong pump-probe (PP) signal from copper, we performed label-free super-resolution imaging of multilayered copper interconnects on a small central processing unit (CPU) chip. The label-free super-resolution DPP optical microscopy opens possibilities for easy, fast, and large-scale electronic inspection in the whole pipeline chain for designing and manufacturing ICs.
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http://dx.doi.org/10.1021/acs.nanolett.1c00403DOI Listing
May 2021

The Effect of Condylar Regeneration After Different Disc Repositioning Surgeries in Adolescents with Skeletal Class II Malocclusion.

J Oral Maxillofac Surg 2021 Mar 22. Epub 2021 Mar 22.

Professor, Department of Oral Surgery, Ninth People's Hospital, Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology; National Clinical Research Center of Stomatology. Shanghai, China.

Purpose: To evaluate the effect of different temporomandibular joint (TMJ) disc repositioning surgeries for the treatment of anterior disc displacement (ADD) in juvenile patients with skeletal Class II malocclusion.

Patients And Methods: Patients (< 20 years, cervical vertebral maturation stage, IV-V) who had bilateral TMJ ADD with skeletal Class II malocclusion were treated by disc repositioning surgery (mini-screw anchor, [MsA]; or opening suturing, [OSu]). Magnetic resonance imaging (MRI) and lateral cephalometric films before and more than 12 months after surgery were collected from all patients. Changes of SNA, SNB, ANB, incisor overjet, pogonion position (pg'-G'), and condylar height were measured before and after surgery in different disc reposition surgeries and compared by statistical analysis.

Results: Eighty-four patients with an average age of 16.44 years and follow-up time of 14.60 months (12 to 33 months) were included in the study. Among them, 16 patients had a mean follow-up of 14.8 months without treatment before disc repositioning. Their condylar height was significantly decreased (P = .004) by MRI measurement. Six patients who had cephalometric films showed significantly decreased SNB (P = .042) and increased overjet (P = .037). After disc repositioning by either OSu (54 cases) or MsA (30 cases), condylar height, SNB were significantly increased and overjet, Pg'-G', ANB were decreased in both groups (P < 0.001). There was more new bone height in OSu than MsA (P = .004), but no significant differences in SNB, ANB, overjet and Pg'-G' between the 2 groups (P > .05). The new bone height was significantly correlated with the surgical method (P = .029), age (P = .015), SNB (P = .008), overjet (P = .048) and pg'-G' (P = .001).

Conclusions: Both types of disc repositioning method can effectively promote condylar regeneration and improve skeletal Class II malocclusion in adolescents with ADD. Disc repositioning by OSu obtained more new bone height than MsA.
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http://dx.doi.org/10.1016/j.joms.2021.03.011DOI Listing
March 2021

Periodontal Inflammation-Triggered by Periodontal Ligament Stem Cell Pyroptosis Exacerbates Periodontitis.

Front Cell Dev Biol 2021 1;9:663037. Epub 2021 Apr 1.

Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Research Unit of Oral and Maxillofacial Regenerative Medicine, Chinese Academy of Medical Sciences, Shanghai, China.

Periodontitis is an immune inflammatory disease that leads to progressive destruction of bone and connective tissue, accompanied by the dysfunction and even loss of periodontal ligament stem cells (PDLSCs). Pyroptosis mediated by gasdermin-D (GSDMD) participates in the pathogenesis of inflammatory diseases. However, whether pyroptosis mediates PDLSC loss, and inflammation triggered by pyroptosis is involved in the pathological progression of periodontitis remain unclear. Here, we found that PDLSCs suffered GSDMD-dependent pyroptosis to release interleukin-1β (IL-1β) during human periodontitis. Importantly, the increased IL-1β level in gingival crevicular fluid was significantly correlated with periodontitis severity. The caspase-4/GSDMD-mediated pyroptosis caused by periodontal bacteria and cytoplasmic lipopolysaccharide (LPS) dominantly contributed to PDLSC loss. By releasing IL-1β into the tissue microenvironment, pyroptotic PDLSCs inhibited osteoblastogenesis and promoted osteoclastogenesis, which exacerbated the pathological damage of periodontitis. Pharmacological inhibition of caspase-4 or IL-1β antibody blockade in a rat periodontitis model lead to the significantly reduced loss of alveolar bone and periodontal ligament damage. Furthermore, Gsdmd deficiency alleviated periodontal inflammation and bone loss in mouse experimental periodontitis. These findings indicate that GSDMD-driven PDLSC pyroptosis and loss plays a pivotal role in the pathogenesis of periodontitis by increasing IL-1β release, enhancing inflammation, and promoting osteoclastogenesis.
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http://dx.doi.org/10.3389/fcell.2021.663037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049442PMC
April 2021

Quantitative Segmentation Analysis of the Radiological Changes by Using ITK-SNAP: Risk Assessment of the Severity and Recurrence of Medication-related Osteonecrosis of the Jaw.

Int J Med Sci 2021 24;18(10):2209-2216. Epub 2021 Mar 24.

Shanghai Ninth People's Hospital, Collage of Stomatology, Shanghai Jiao Tong University School of Medicine (200011); National Clinical Research Center of Oral Disease; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Department of Oral Surgery.

Medication-related osteonecrosis of the jaw (MRONJ) severely impairs patients' quality of life and is remarkably refractory to treatment. There are lots of studies about identification of the radiographic features of MRONJ, yet reports about quantitative radiographic analysis for the risk assessment of the severity and recurrence of MRONJ are rarely heard. The aim of this study was to investigate the volumes of osteolytic lesions and radiodensity values of osteosclerotic lesions in MRONJ patients by using ITK-SNAP for severity prediction and prognosis evaluation. Of 78 MRONJ patients (78 lesions) involved in this retrospective study, 53 were presented as osteolytic lesions and 25 were presented as osteosclerotic changes alone. Comprehensive CBCT images, demographics and clinical data of patients were investigated. The volumetric analysis and radiodensity measurement were performed by ITK-SNAP. SPSS 25.0 were used for statistical analysis. The osteolytic lesion volumes in MRONJ patients receiving intravenous bisphosphonates (P=0.004) and patients without osteoporosis (P=0.027) were significantly large. No significant correlation between the volumes and bisphosphonates duration was found (P=0.094). The radiodensity values of osteosclerotic lesions was significantly correlated with bisphosphonates duration (P=0.040). The surrounding area of post-surgical lesions in MRONJ patients with recurrence showed significantly great radiodensity values (P=0.025). No significant correlation between the radiodensity values and the transformation from osteosclerotic lesions to osteolytic lesions was observed (P=0.507). MRONJ patients receiving intravenous bisphosphonates develop into large volumes of osteolytic lesions more easily. Long-term bisphosphonates duration is possibly related with higher bone density of osteosclerotic lesions, while higher density is not associated with the transformation from osteosclerotic lesions to osteolytic lesions. A rise of bone mineral density nearby post-surgical lesions is probably a predictor for MRONJ recurrence.
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http://dx.doi.org/10.7150/ijms.56408DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040413PMC
March 2021

Determining the optimal magnetic resonance imaging sequences for the efficient diagnosis of temporomandibular joint disorders.

Quant Imaging Med Surg 2021 Apr;11(4):1343-1353

Department of Oral Surgery, Ninth People's Hospital, Shanghai Key Lab of Stomatology, Shanghai, China.

Background: To compare and analyze nine MRI sequences of the TMJ and determine the optimum sequence for the rapid diagnosis of TMDs so as to develop new clinical guidelines.

Methods: Twenty young volunteers (a total of 40 joints) aged 22-26 years were recruited. Three basic sequences, T-weighted imaging (TWI), T-weighted imaging (TWI), and proton density-weighted imaging (PDWI), together with three positions, oblique sagittal (OSag) with closed mouth, oblique coronal (OCor) with closed mouth, and OSag with opened mouth, were selected in combination for testing. In the OCor position, four regions of interest (ROIs), the condyle (C), the disc (D), the disc outside (DO), and fat (F), were analyzed. For the OSag with closed mouth position and the OSag with opened mouth position sequences, the four ROIs were the condyle (C), the disc (D), the disc ahead (DA), and the disc rear (DR). The signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR), and signal intensity ratio (SIR) were calculated and analyzed using independent sample -tests and one-way analysis of variance. Two senior radiologists scored the images of the nine MRI sequences subjectively and selected three optimal sequences. Using the three selected sequences, 1479 patients with anterior disc displacement with reduction (ADDwR) or anterior disk displacement without reduction (ADDwoR) were evaluated by comparing the preoperative TMJ MRI with the outcomes of the maxillofacial arthroscopy or open surgery.

Results: The TWI sequence showed the highest SNR while the TWI group had the lowest SNR. The ROIs of the TWI group had the highest CNR and SIR values in the OCor and OSag sequences. In the OCor sequence, the value for the SIR F/DO group was higher than the SIR C/D and SIR C/DO values. Using subjective analysis to evaluate the quality of the scans, the highest total scores were obtained for the OSag TWI with opened mouth and OSag PDWI with closed mouth sequences. From the objective and subjective analysis, the three optimal sequences selected were OSag PDWI, OCor TWI with closed mouth, and OSag TWI with opened mouth. In patients with anterior disc displacement, the comparisons of the surgery and the selected MRI sequences indicated that the total diagnostic accuracy of the MRI was 96.3% (1,425/1,479 cases). For patients with ADDwoR, the diagnostic accuracy was 98.5% (1,372/1,393 cases), and for those with ADDwR it was 61.6% (53/86 cases). There were significant differences between the ADDwoR and ADDwR groups (χ=312.92, P<0.01).

Conclusions: The three optimal MRI sequences for the rapid and efficient diagnosis of TMD were determined to be OSag PDWI, OCor TWI with closed mouth, and OSag TWI with opened mouth.
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http://dx.doi.org/10.21037/qims-20-67DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930691PMC
April 2021

Performance of Interferon-Gamma Release Assays in the Diagnosis of Nontuberculous Mycobacterial Diseases-A Retrospective Survey From 2011 to 2019.

Front Cell Infect Microbiol 2020 18;10:571230. Epub 2021 Feb 18.

Shanghai Clinical Research Center for Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.

There is an urgent need for precise diagnosis to distinguish nontuberculous mycobacterial (NTM) diseases from pulmonary tuberculosis (PTB) and other respiratory diseases. The aim of this study is to evaluate the diagnostic performance of Interferon-gamma (IFN-γ) release assays (IGRAs), including antigen-specific peripheral blood-based quantitative T cell assay (T-SPOT.TB) and QuantiFERON-TB-Gold-Test (QFT-G), in differentiating NTM infections ( = 1,407) from culture-confirmed PTB ( = 1,828) and other respiratory diseases ( = 2,652). At specie level, 2.56%, 10.73%, and 16.49% of NTM-infected patients were infected by , , and with - complex (MAC), respectively. Valid analyses of T-SPOT.TB (ESAT-6, CFP-10) and QFT-G were available for 37.03% and 85.79% in NTM-infected patients, including zero and 100% (36/36) of infection, 21.85% (33/151) and 92.05% (139/151) of infection, and 17.67% (41/232) and 91.24% (211/232) of MAC infection. Based on means comparisons and further ROC analysis, T-SPOT.TB and QFT-G performed moderate accuracy when discriminating NTM from PTB at modified cut-off values (ESAT-6 < 4 SFCs, CFP-10 < 3 SFCs, and QFT-G < 0.667 IU/ml), with corresponding AUC values of 0.7560, 0.7699, and 0.856. At species level of NTM, QFT-G effectively distinguished between MAC (AUC=0.8778), (AUC=0.8834) or (AUC=0.8783) than T-SPOT.TB. No significant differences in discriminatory power of these three IGRA tools were observed when differentiating NTM and Controls. Our results demonstrated that T-SPOT.TB and QFT-G were both efficient methods for differentiating NTM disease from PTB, and QFT-G possessed sufficient discriminatory power to distinguish infections by different NTM species.
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http://dx.doi.org/10.3389/fcimb.2020.571230DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930076PMC
June 2021

The magnetic resonance imaging evaluation of condylar new bone remodeling after Yang's TMJ arthroscopic surgery.

Sci Rep 2021 Mar 4;11(1):5219. Epub 2021 Mar 4.

Department of Oral Maxillofacial Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200011, China.

To evaluate the post-operative condylar bone remodeling after the treatment of Yang's arthroscopic surgery. Consecutive cases from Jan 2017 to May 2018 that received Yang's arthroscopic surgery were included in this study, the TMJ MRI examinations were performed preoperatively and postoperatively (follow up for 1 year or more), and condylar bone remodeling was estimated. A total of 229 patients (29 male and 200 female) were included in the study, 161 patients had new bone formation, and the average age was 17.5 ± 2.1a. There was no new bone formation in 68 patients with an average age of 24.5 ± 0.7a. The percentage of new bone formation patients in 10-15 years of age was 94.33% and decreases as the age increases. In the position of new bone formation, the posterior slope of condyle was the most (129 joints), the second was the top of condyle (54 joints), the third was around condyle (33 joints), only 25 joints had new bone on the anterior slope of condyle. After TMJ arthroscopic surgery, the condyle has the ability to form new bone, and the younger the age, the stronger the ability of new bone formation. The formation of new bone was most in posterior slope and least in anterior slope of condyle.
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http://dx.doi.org/10.1038/s41598-021-84591-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7933332PMC
March 2021

The investigation of surface composition of nitrogen-doped niobium for superconducting RF cavities.

Nanotechnology 2021 Mar 3. Epub 2021 Mar 3.

Sichuan University, Chengdu, Sichuan, CHINA.

Systematic analysis of the surface morphology, crystalline phase, chemical composition and elemental distribution along depth for nitrogen-doped niobium was carried out through different ways of characterization, including SEM, AFM, GIXRD, RBS and layer-by-layer XPS analysis. The results showed that, after nitrogen doping, the surface was covered by densely distributed trigonal precipitates with average crystallite size of 32 ± 8 nm, in line with the calculation result (29.9 nm) of nitrogen-enriched β-Nb2N from GIXRD, demonstrating the phase composion of trigonal precipitates. The depth analysis through RBS and XPS indicated that β-Nb2N was dominant in the topmost 9.7 nm and extended to depth of 575 nm with gradually decreased content. In addition, the successive change of naturally oxidized states of niobium after nitrogen doping along depth was revealed along depth. It was interesting to find that oxygen diffusion depth could be moderately enhanced by nitridation process. These results established the near-surface phase composition of nitrided niobium, which was of great significance for evaluating the effect of nitrogen doping and further understanding the Q improvement of the SRF cavities.
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http://dx.doi.org/10.1088/1361-6528/abeb99DOI Listing
March 2021

[Progress in enhancing electron transfer rate between exoelectrogenic microorganisms and electrode interface].

Sheng Wu Gong Cheng Xue Bao 2021 Feb;37(2):361-377

School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.

Exoelectrogenic microorganisms are the research basis of microbial electrochemical technologies such as microbial fuel cells, electrolytic cells and electrosynthesis. However, their applications are restricted in organic degradation, power generation, seawater desalination, bioremediation, and biosensors due to the weak ability of biofilm formation and the low extracellular electron transfer (EET) efficiency between exoelectrogenic microorganisms and electrode. Therefore, engineering optimization of interaction between exoelectrogenic microorganisms and electrode interface recently has been the research focus. In this article, we review the updated progress in strategies for enhancing microbe-electrode interactions based on microbial engineering modifications, with a focus on the applicability and limitations of these strategies. In addition, we also address research prospects of enhancing the interaction between electroactive cells and electrodes.
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http://dx.doi.org/10.13345/j.cjb.200281DOI Listing
February 2021

Injectable hydrogel with MSNs/microRNA-21-5p delivery enables both immunomodification and enhanced angiogenesis for myocardial infarction therapy in pigs.

Sci Adv 2021 Feb 24;7(9). Epub 2021 Feb 24.

National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, Department of Oral Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.

Current therapeutic strategies such as angiogenic therapy and anti-inflammatory therapy for treating myocardial infarction have limited success. An effective approach may benefit from resolution of excessive inflammation combined with enhancement of angiogenesis. Here, we developed a microRNA-21-5p delivery system using functionalized mesoporous silica nanoparticles (MSNs) with additional intrinsic therapeutic effects. These nanocarriers were encapsulated into an injectable hydrogel matrix ([email protected]/miR-21-5p) to enable controlled on-demand microRNA-21 delivery triggered by the local acidic microenvironment. In a porcine model of myocardial infarction, we demonstrated that the released MSN complexes notably inhibited the inflammatory response by inhibiting the polarization of M1 macrophage within the infarcted myocardium, while further microRNA-21-5p delivery by MSNs to endothelial cells markedly promoted local neovascularization and rescued at-risk cardiomyocytes. The synergy of anti-inflammatory and proangiogenic effects effectively reduced infarct size in a porcine model of myocardial infarction.
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http://dx.doi.org/10.1126/sciadv.abd6740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904259PMC
February 2021

Preliminary Study of Standard Artificial Temporomandibular Joint Replacement With Preservation of Muscle Attachment.

J Oral Maxillofac Surg 2021 May 17;79(5):1009-1018. Epub 2020 Dec 17.

Resident, Department of Oral Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology. National Clinical Research Center of Stomatology, Shanghai, China.

Purpose: To evaluate the effect of preserving the muscle attachments when performing standard artificial temporomandibular joint replacement (TJR).

Patients And Methods: The clinical and radiological imaging data of patients who underwent standard artificial TJR with and without preservation of lateral pterygoid muscle (LPM) and masseter muscle attachments from January 2017 to December 2019 were collected. The maximum interincisal opening (MIO), lateral excursions and protrusion distances, visual analogue scale (VAS) scores of pain, diet, and quality of life (QoL) were recorded before the operation, and 1, 3, 6, and 12 months after the operation. The volumes of LPM and masseter muscles were measured and analyzed by computed tomography (CT) scans.

Results: Twenty-seven patients with 36 joints were included in the study. Among them, 11 joints had muscle attachment preserved, and 25 had no muscle attachment preserved. After surgery, the MIO, lateral excursions, and scores of diet, pain, and QoL in the preserved muscle attachment group were significantly better than those in the unpreserved group (P < .05). The measurement volumes of LPM and masseter muscles in the preserved group were significantly larger than that in the unpreserved group (P < .05). Changes of lateral excursion from the TJR side with and without LPM preservation were statistically correlated with the LPM volume (P < .05).

Conclusion: Preserving muscle attachment for the standard artificial TJR is beneficial to the recovery of postoperative mandibular function.
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http://dx.doi.org/10.1016/j.joms.2020.12.012DOI Listing
May 2021

Timing of force application on buccal tooth movement into bone-grafted alveolar defects: A pilot study in dogs.

Am J Orthod Dentofacial Orthop 2021 Feb 17;159(2):e123-e134. Epub 2020 Dec 17.

Department of Oral Surgery, Shanghai Ninth People's Hospital, Shanghai Key Laboratory of Stomatology, and Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University School of Medicine, National Clinical Research Center for Oral Diseases, and National Clinical Research Center of Stomatology, Shanghai, China. Electronic address:

Introduction: The aim of this pilot study was to evaluate the effect of the timing of postoperative orthodontic force application on bone remodeling during tooth movement into surgical alveolar defects with bone grafts in beagle dogs.

Methods: Six beagle dogs underwent surgery for buccal dehiscence-type defects (width, 5 mm; height, 6 mm) on the distal root of maxillary second premolars bilaterally for 12 defects. After 1-month healing, bone-augmentation procedures were undertaken at the dehiscence defects. The second premolars were protracted buccally for 6 weeks into the surgical sites immediately (F-0), at 4 weeks (F-4), or 8 weeks (F-8) after grafting. Orthodontic tooth movement was monitored using digital models. Remodeling of alveolar bone was evaluated by histology, histomorphometry, immunohistochemistry, microcomputed tomography, and fluorescence microscopy.

Results: Group F-0 showed significant expansion (mean, 2.42 mm) and tipping (mean, 9.03°) after completing orthodontic tooth treatment. The vertical bone defect was significantly lower in groups F-4 and F-8 than that in group F-0 (mean, 2.1, 2.7, and 4.5 mm, respectively). In group F-4, the formation of new bone and mineralization were significantly greater than those in groups F-0 and F-8 (P <0.05). Group F-4 showed a minimal amount of bone-material remnants. Immunohistochemistry showed the highest expression of collagen-1 and osteopontin in group F-4, followed by group F-8 and group F-0, which demonstrated high osteoblast activity and enhanced bone remodeling in group F-4.

Conclusions: Orthodontic force application at 4 weeks after an augmentation procedure provided the best functional stimulation for an alveolar bone graft. This strategy enhanced new-bone regeneration and degradation of bone substitutes and, eventually, promoted bone remodeling in the bone-grafted area.
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http://dx.doi.org/10.1016/j.ajodo.2020.09.010DOI Listing
February 2021

Clinical and radiological outcomes of Chinese customized three-dimensionally printed total temporomandibular joint prostheses: A prospective case series study.

J Plast Reconstr Aesthet Surg 2021 Jul 20;74(7):1582-1593. Epub 2020 Nov 20.

Department of Oral Surgery, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology. Shanghai, China. Electronic address:

Purpose: Temporomandibular joint (TMJ) diseases are highly prevalent in China. However, no commercialized custom-made prostheses are available now. This study introduces a three-dimensionally (3D) printed customized total TMJ prosthesis manufactured by a standardized workflow.

Materials And Methods: Consecutive patients with end-stage TMJ diseases were recruited from Jan 2018 to Sep 2018. The computed tomography (CT) data for patients were obtained and transformed into the Mimics 18.0 software preoperatively for designing of prostheses and digital templates. 3D printing, friction spot welding and computer-assisted manufacture (CAM) were used to fabricate different components of the prosthesis. The clinical and radiographic evaluations were performed postoperatively.

Results: A series of 9 patients were included. All the prostheses were placed smoothly and fixed stably during surgical procedure. Without severe postoperative complications, all patients exhibited significant improvements in maximum mouth opening, pain, diet, and mandibular function, with good facial symmetry. For the whole prosthesis, the average mean deviation was 0.432 mm (range: from 0.279 to 0.561 mm).

Conclusions: This study suggests that Chinese customized 3D-printed total TMJ prostheses produces excellent short-term clinical outcomes, with high accuracy in implantation.
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http://dx.doi.org/10.1016/j.bjps.2020.10.108DOI Listing
July 2021

Activation of mesenchymal stem cells promotes new bone formation within dentigerous cyst.

Stem Cell Res Ther 2020 11 10;11(1):476. Epub 2020 Nov 10.

Department of Oral Surgery, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Centre for Oral Diseases, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Dentigerous cyst (DC) is a bone destructive disease and remains a challenge for clinicians. Marsupialization enables the bone to regenerate with capsule maintaining, making it a preferred therapeutic means for DC adjacent to vital anatomical structures. Given that capsules of DC are derived from odontogenic epithelium remnants at the embryonic stage, we investigated whether there were mesenchymal stem cells (MSCs) located in DC capsules and the role that they played in the bone regeneration after marsupialization.

Methods: Samples obtained before and after marsupialization were used for histological detection and cell culture. The stemness of cells isolated from fresh tissues was analyzed by morphology, surface marker, and multi-differentiation assays. Comparison of proliferation ability between MSCs isolated from DC capsules before (Bm-DCSCs) and after (Am-DCSCs) marsupialization was evaluated by Cell Counting Kit-8 (CCK-8), fibroblast colony-forming units (CFU-F), and 5'-ethynyl-2'-deoxyuridine (EdU) assay. Their osteogenic capacity in vitro was detected by alkaline phosphatase (ALP) and Alizarin Red staining (ARS), combined with real-time polymerase chain reaction (RT-PCR) and immunofluorescence (IF) staining. Subcutaneous ectopic osteogenesis as well as cranial bone defect model in nude mice was performed to detect their bone regeneration and bone defect repairability.

Results: Bone tissue and strong ALP activity were detected in the capsule of DC after marsupialization. Two types of MSCs were isolated from fibrous capsules of DC both before (Bm-DCSCs) and after (Am-DCSCs) marsupialization. These fibroblast-like, colony-forming cells expressed MSC markers (CD44+, CD90+, CD31-, CD34-, CD45-), and they could differentiate into osteoblast-, adipocyte-, and chondrocyte-like cells under induction. Notably, Am-DCSCs performed better in cell proliferation and self-renewal. Moreover, Am-DCSCs showed a greater osteogenic capacity both in vitro and in vivo compared with Bm-DCSCs.

Conclusions: There are MSCs residing in capsules of DC, and the cell viability as well as the osteogenic capacity of them is largely enhanced after marsupialization. Our findings suggested that MSCs might play a crucial role in the healing process of DC after marsupialization, thus providing new insight into the treatment for DC by promoting the osteogenic differentiation of MSCs inside capsules.
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http://dx.doi.org/10.1186/s13287-020-01999-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7653780PMC
November 2020

Combined Administration of Bisphosphonates, Chemotherapeutic Agents, and/or Targeted Drugs Increases the Risk for Stage 3 Medication-Related Osteonecrosis of the Jaw: A 4-Year Retrospective Study.

Biomed Res Int 2020 15;2020:5847429. Epub 2020 Oct 15.

National Clinical Research Center for Oral Disease, Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, Department of Oral Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, 200011, China.

Objectives: Patients with stage 3 medication-related osteonecrosis of the jaw (MRONJ) suffer from severe complications. Chemotherapeutic agents and targeted drugs are considered to be associated with the development of MRONJ. However, little is known regarding the association of those agents with stage 3 MRONJ. The purpose of this study is to analyze the comprehensive medication history of patients with advanced-stage MRONJ (stage 2 and stage 3) and evaluate the possible risk factors for stage 3 MRONJ. . Sixty patients with advanced-stage MRONJ were involved in this retrospective study. Patients with developmental maxillofacial anomalies, previous radiation in the head and neck areas, and jaw bone tumors were excluded from the study. All patients were divided into two groups by their MRONJ stage (stage 2 or stage 3). Demographic and clinical characteristics, comprehensive medication data (bisphosphonates, chemotherapeutic agents, targeted drugs, and immunosuppressive agents), and results of serological biomarkers were recorded and compared between two groups. Univariate and multivariate logistic regressions were performed by SPSS 25.0 for evaluating risk factors of stage 3 MRONJ.

Results: Our results indicate that chemotherapy (adjusted OR = 3.43; 95% CI: 1.03 to 11.38), targeted drugs (adjusted OR = 3.69; 95% CI: 1.06 to 12.80), and maxillary lesions (adjusted OR = 4.26; 95% CI: 1.19 to 15.23) increase the risk of stage 3 MRONJ.

Conclusion: The outcome of this study justifies that chemotherapeutic agents and targeted drugs are probably risk factors for stage 3 MRONJ. In addition, the osteonecrosis in maxilla is more easily to develop into stage 3 MRONJ. Intense clinical observation is recommended in MRONJ patients with maxillary osteonecrosis and in those who concurrently administered bisphosphonates, chemotherapeutic agents, and/or targeted drugs. This trial is registered with ChiCTR2000032428.
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http://dx.doi.org/10.1155/2020/5847429DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584964PMC
May 2021

Biomechanical analysis of costochondral graft fracture in temporomandibular joint replacement.

Sci Rep 2020 10 20;10(1):17754. Epub 2020 Oct 20.

Department of Oral Surgery, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, and Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, 200011, China.

This study is the first attempt to explore the reason of costochondral graft fracture after lengthy mandible advancement and bilateral coronoidectomy by combining finite element analysis and mechanical test. Eleven groups of models were established to simulate costochondral graft reconstruction in different degrees of mandible advancement, ranging from 0 to 20 mm, in 2 mm increment. Force and stress distribution in the rib-cartilage area were analyzed by finite element analysis. Mechanical test was used to evaluate the resistance of the rib-cartilage complex. Results showed a sharp increase in horizontal force between 8 and 10 mm mandible advancement, from 26.7 to 196.7 N in the left side, and continue increased after 10 mm, which was beyond bone-cartilage junction resistance according to mechanical test. Therefore, we concluded that bilateral reconstruction with coronoidectomy for lengthy mandible advancement (≥ 10 mm) may lead to prominent increase in shear force and result in a costal-cartilage junction fracture, in this situation, alloplastic prosthesis could be a better choice. We also suggested that coronoidectomy should be carefully considered unless necessary.
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http://dx.doi.org/10.1038/s41598-020-74548-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7576606PMC
October 2020

The Correlation Between Severity of Postoperative Hypocalcemia and Perioperative Mortality in Chromosome 22q11.2 Microdeletion (22q11DS) Patient After Cardiac-Correction Surgery: A Retrospective Analysis.

Heart Surg Forum 2020 Aug 3;23(5):E549-E554. Epub 2020 Aug 3.

Department of Cardiothoracic Surgery, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.

Objectives: The aim of our study was to elucidate the association between severity of postoperative hypocalcemia and the prognosis of the patients with 22q11DS.

Methods: Data retrospectively were collected from 23 children with 22q11DS who underwent cardiac correction surgery. Area under the receiver operating characteristic curve (AUC) and diagnostic odds ratio were calculated to determine the tendency of perioperative mortality rate, according to the minimum levels of serum calcium and the duration of hypocalcemia. A novel risk assessment system for perioperative mortality was established according to these valid parameters.

Results: The death group had lower minimum levels of serum calcium and longer duration of hypocalcemia. The AUC of minimum levels of serum calcium was 0.912 (95% CI: 0.753-1; P = .003) and qualified its high accuracy for perioperative mortality. The AUC of duration of hypocalcemia was 0.804 (95% CI: 0.561-1; P = .03) and qualified its moderate accuracy. The tendency analyses also indicated the correlation between these two parameters and perioperative mortality. Based on the cut-off values from ROC analysis, a novel risk assessment system for perioperative mortality was established according to these two parameters. The patients with the lowest serum calcium level <0.885 mmol/L or duration of the hypocalcemia > 90.33 hours would be sorted into a high-risk group; others were divided into a low-risk group. The diagnostic odds ratio for this assessment system was 143(95% CI: 5.13-3982.52). No significant difference was found with regard to patient age, weight, preoperative serum total calcium, cardiopulmonary bypass (CPB) time, and aortic cross-clamp time between the high- and low-risk groups.

Conclusions: The minimum levels of serum calcium and duration of hypocalcemia were valid predictors for preoperative mortality of 22q11DS patients.
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http://dx.doi.org/10.1532/hsf.2957DOI Listing
August 2020

Effect of Attapulgite-Doped Electrospun Fibrous PLGA Scaffold on Pro-Osteogenesis and Barrier Function in the Application of Guided Bone Regeneration.

Int J Nanomedicine 2020 11;15:6761-6777. Epub 2020 Sep 11.

Department of Oral Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiaotong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, People's Republic of China.

Purpose: Guided bone regeneration (GBR) therapy, which is a widely used technique in clinical practice and is effective in improving the repair of alveolar bone defects or bone mass deficiency regeneration, requires the use of membrane materials with good biocompatibility, barrier function, rigidity matching the space maintenance ability, economic benefits and excellent clinical applicability. The aim of this study was to develop an electrospun attapulgite (ATT)-doped poly (lactic-co-glycolic acid) (PLGA) scaffold (PLGA/ATT scaffold) as a novel material for GBR applications.

Methods And Results: Scanning electron microscopy (SEM) and X-ray diffraction (XRD) were used to determine the morphology and the crystalline structure of the PLGA/ATT scaffolds, respectively. Porosity and contact-angle measurements were also carried out to further characterize the physical properties of the PLGA/ATT scaffolds. The results of in vitro studies showed that bone marrow mesenchymal stem cells (BMSCs) attached more readily to and spread better over the PLGA/ATT scaffolds than the Bio-Gide membrane. Furthermore, in the in vitro osteoinductive experiments with BMSCs, the PLGA/ATT scaffolds were found to enhance the activity of alkaline phosphatase (ALP), promote the formation of mineralized bone nodules, and up-regulate the expression of several osteogenic markers-namely, runt-related transcription factor 2, alkaline phosphatase, osteopontin, and osteocalcin-which are similar to the effects of the Bio-Gide membrane. Further, in in vivo studies, the results of sequential fluorescent labeling, micro-computed tomography, and histological analysis suggest that using the PLGA/ATT scaffolds for repairing V-shaped buccal dehiscence on a dog's tooth root improved bone regeneration, which is not only similar to the result obtained using the Bio-Gide membrane but also much better than that obtained using PLGA scaffolds and the negative control.

Conclusion: To achieve satisfactory therapeutic results and to lower the cost of GBR treatment, this study provided a promising alternative material of bio-degradable membrane in clinical treatment.
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http://dx.doi.org/10.2147/IJN.S244533DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7494386PMC
November 2020

Isomerization and Degradation of Levoglucosan via the Photo-Fenton Process: Insights from Aqueous-Phase Experiments and Atmospheric Particulate Matter.

Environ Sci Technol 2020 10 19;54(19):11789-11797. Epub 2020 Sep 19.

School of Applied Meteorology, Nanjing University of Information Science and Technology, Nanjing 210044, China.

So far, studies on the conversion of stereochemistry under photo-Fenton conditions and their atmospheric implication are still rare. Here, we found that the biomass burning marker, the chiral compound levoglucosan (L), undergoes oxidative degradation under photo-Fenton conditions and can be isomerized into mannosan (M) and galactosan (G) simultaneously. Among the formic acid, acetic acid, and oxalic acid in the degradation products of levoglucosan, it was found that the yield of formation of formic acid in the photo-Fenton pathway can be as high as 86%. It is worth noting that both levoglucosan and its isomers are present in the atmosphere and their concentrations are strongly correlated. At the same time, the range of their concentration ratios, L/(G + M), measured in the photo-Fenton experiments in the laboratory was found to agree well with that measured in atmospheric PM samples. However, the sources of L, G, and M in the atmosphere are complex, and the photo-Fenton reaction may be an essential pathway for the distribution of L, G, and M in the atmosphere.
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http://dx.doi.org/10.1021/acs.est.0c02499DOI Listing
October 2020

Bioactivities and Molecular Characterizations of the Polysaccharide(s) from Ultrasonic-Circulating Extracts of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes).

Int J Med Mushrooms 2020 ;22(5):455-466

Fujian University of Traditional Chinese Medicine, Fuzhou, China.

Total polysaccharide (i.e., GLP-UCE) was effectively isolated from the crude polysaccharide extract powder of Ganoderma lucidum by ultrasonic-circulating extraction, which was separated into three fractions (i.e., GLP-U40, GLP-U60, and GLP-U80) by ethanol fractional precipitation. The detection of antioxidant enzymes (GSH-Px, SOD) and oxidation metabolites (MDA, LF); liver function test of ALT, AST, and LDH; and western blot for antioxidant proteins of GSH-Px and MMP-2 showed that the GLP-UCE displayed a favorable in vivo antioxidant and hepatoprotective activities for reducing the oxidative damage in CCl4-hepatopathy SD rats. The GLP-UCE and its fractions were analyzed and compared by Fourier transform infrared (FT-IR), high-performance liquid chromatography, high-performance gel permeation chromatography, and antioxidant activity in vitro assay. These studies revealed that the fraction GLP-U80 exhibited stronger antioxidant activities in vitro than that of GLP-UCE and other fractions (p
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http://dx.doi.org/10.1615/IntJMedMushrooms.2020034227DOI Listing
March 2021

The effect of arthroscopic disc repositioning on facial growth in juvenile patients with unilateral anterior disc displacement.

J Craniomaxillofac Surg 2020 Aug 17;48(8):765-771. Epub 2020 Jun 17.

Department of Oral Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Key Laboratory of Stomatology, Shanghai, 200011, China.

Objective: To investigate the effect of arthroscopic disc repositioning on condylar development and mandibular asymmetry in juvenile patients with unilateral temporomandibular joint (TMJ) anterior disc displacement (ADD).

Patients And Methods: A retrospective cohort study was conducted on unilateral juvenile anterior disc displacement (UJADD) patients of 10-20 years of age treated in Shanghai Ninth People's Hospital from 2010 to 2017. The primary predictor was TMJ arthroscopic disc repositioning surgery, according to which patients were divided into a surgery group and a control group. Initial and re-examined (with at least 6-month interval) magnetic resonance imaging (MRI) and posteroanterior cephalometric radiographs (PA) were collected. The condylar heights on both sides of the TMJ were measured by MRI and the difference between them was calculated. Menton (Me) deviation was measured based on PA. Changes in condylar height difference and Me deviation were compared between the two groups.

Results: A total of 108 UJADD patients were collected in this study, including 55 patients in the surgery group and 53 patients in the control group. There were no significant differences in age, sex and follow-up time between the two groups. The condylar height difference was (-0.61 ± 2.02) mm in surgery group and (1.68 ± 1.73) mm in control group (P < 0.0001); while the Me deviation was (-1.00 ± 1.15) mm in surgery group and (1.81 ± 1.96) mm in control group (P < 0.0001), indicating that TMJ arthroscopic disc repositioning surgery could significantly improve condylar height difference and Me deviation.

Conclusions: UJADD should be treated as early as possible, especially in juvenile patients, in order to improve condylar development and avoid jaw deformities.
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http://dx.doi.org/10.1016/j.jcms.2020.05.016DOI Listing
August 2020